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identification of intestinal bacterial flora in rhipicephalus microplus ticks by conventional methods and pcr-dgge analysis.in this study, we have analyzed the intestinal microbial flora associated with rhipicephalus microplus ticks using both culture-dependent and independent methods based on pcr and denaturing gradient gel electrophoresis (pcr-dgge). the r. microplus ticks were collected from cattle and goats in jiangxi, hunan and guizhou provinces of china. three distinct strains of bacteria were isolated using culture-dependent methods: staphylococcus simulans, bacillus subtilis and bacillus flexus strain. ninete ...201525784070
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