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isolation, molecular characterization and evaluation of the pathogenicity of a porcine rotavirus isolated from jiangsu province, china.in this study, a porcine rotavirus was isolated from a fecal sample from a diarrheic piglet in jiangsu province, china. rotavirus-specific cytopathic effects were observed after 12 blind passages on ma-104 cells, yielding a virus titer of 10(6.125) tcid50/ml. by applying an 80 % nucleotide cutoff value and the rotac(2.0) automated genotyping tool, the vp4 genotype of the new isolate was identified as p[7]. the vp7 genotype was identified as g[9], lineage vi, and sublineage c. experimentally infe ...201525742930
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