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clostridium perfringens toxin types from freshwater fishes in one water reservoir of shandong province of china, determined by pcr.four hundred and twenty intestinal content samples (not including intestinal tissues) of freshwater fishes (60 silver carps, 100 carps, 100 crucian carps, 60 catfishes and 100 zaieuws) caught from one water reservoir were examined bacteriologically for the occurrence of c. perfringens. isolates were examined by polymerase chain reaction (pcr) for genes encoding the four lethal toxins (alpha, beta, epsilon and iota) for classification into toxin types and for genes encoding enterotoxin and the no ...200818717056
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