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brain acetylcholinesterase as a marine pesticide biomarker using brazilian fishes.brain acetylcholinesterase (ache) of some fishes from the coast of rio de janeiro state was studied as a possible pesticide biomarker in marine environmental monitoring. ache specific activity in brain varied from 145 to 530 u/g of proteins and the michaelis-menten constant (k(m)) for acetylthiocholine varied from 104 to 291 microm among the 20 species studied. the enzyme sensitivity to methyl paraoxon, evaluated by the inhibition kinetic constants, shows that some species (paralonchurus brasili ...200717118441
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