Publications

expression, refolding, and activity of a recombinant nonhemorrhagic snake venom metalloprotease.	snake venoms are rich sources of proteases that strongly affect the vascular system, by promoting blood coagulation, hemorrhage, and fibrinolysis. hemorrhagic activity is mostly due to the enzymatic action of metalloproteases on capillary basement membrane components, such as collagen iv, laminin, and fibronectin. a few low-molecular-weight snake venom metalloproteases (svmp) have been described as being devoid of hemorrhagic activity, but they have strong direct-acting fibrinolytic activity tha ...	2000	10833388
expression of an active recombinant lysine 49 phospholipase a(2) myotoxin as a fusion protein in bacteria.	acl myotoxin (aclmt) is a k49 phospholipase a(2)-like protein isolated from the venom of the snake agkistrodon contortrix laticinctus (broad-banded copperhead) that induces necrosis of skeletal muscle. we have previously cloned and sequenced the cdna coding for aclmt from a venom gland cdna library. in order to perform structure and function studies, we have developed an expression system for production of aclmt as a fusion protein with maltose binding protein (mbp) from the periplasm of bacteri ...	2001	11478968