Publications

Prevalence of porcine circovirus-like agent P1 in Jiangsu, China.	ABSTRACT: Recently, we identified a novel porcine circovirus type 2-like agent P1 isolate from swine. The present study represents the first survey of P1 prevalence in swine herds from Jiangsu, China, by using PCR targeting the complete genome of P1. Prevalences of 50% and 19% were found among 6 herds and 248 animals, respectively. The results indicate a high prevalence of P1 in China pig populations.	2011	22171842
a novel porcine circovirus-like agent p1 is associated with wasting syndromes in pigs.	a novel porcine pathogen tentatively named p1, which was obtained from the sera of the pigs exhibiting clinical signs of postweaning multisystemic wasting syndrome (pmws) experimentally caused the classical clinic signs and pathologic lesions of the disease in pigs by direct in vivo injection with p1 dna plasmids. twenty colostrum-fed (cf) pigs that were free of pcv2 and p1 at 1 month of age were randomly designated equally to two groups. group 1 pigs were each injected with 400 µg of the cloned ...	2012	22936978
transcriptional analysis of porcine circovirus-like virus p1.	recently identified porcine circovirus-like virus p1 has the smallest dna viral genome. in this study, we identified the viral genes and their corresponding mrna transcripts.	2014	25440084
development of real-time pcr assay for detection of porcine circovirus-like virus p1 in domestic pigs in china.	the porcine circovirus-like agent p1 is a newly discovered dna virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. p1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. this study aims to develop a rapid, sensitive and specific method to detect p1.	2015	26404908
function analysis of proteins encoded by orfs 1 to 8 of porcine circovirus-like virus p1 by microarray assay.	porcine circovirus-like agent p1 is a newly discovered virus containing a single-strand circular genome. the genome of p1 is a dna molecule of 648 nucleotides which contains eight open reading frames (orfs) that probably encode potential proteins or polypeptides. thus it is very important to clarify these proteins' function. here we provide the methods and analysis of microarray data in detail to characterize the transcriptome profile of p1 with and without the orf. the relevant microarray data ...	2015	26697373
development and application of an indirect enzyme-linked immunosorbent assay using recombinant truncated cap protein for the diagnosis of porcine circovirus-like virus p1.	porcine circovirus-like virus p1 is a newly discovered virus. to date, there has been no specific serological assay for use in the diagnosis of p1 infection.	2016	26786046
genome sequence of a porcine circovirus-like virus p1 mutant in china.	a new porcine circovirus-like virus p1 mutant was isolated and sequenced in china in 2015. this p1 mutant has a mutation in its orf1-encoded capsid protein that results in its elongation by eight amino acids.	2017	27744634
molecular characterization of porcine circovirus-like virus p1 in eastern china.	a large-scale epidemiological study of porcine circovirus-like virus p1 was carried out from 2007 to 2015 in china, which revealed a high level of p1 infection. thirty-nine sequences obtained from pigs in eastern china showed remarkable genetic diversity, with the p1 predominant sequences and the mutant p1 with orf1 or orf2 containing an additional amino-acid extension at the c-terminus. this information may be useful for understanding the evolution of p1 circovirus-like virus.	2017	27986553
porcine circovirus-like virus p1 in cattle, goats and rabbits in china.	porcine circovirus-like virus p1, a novel single-stranded circular dna virus, is a potential causative agent of post-weaning multisystemic wasting syndrome in swine. in this study, we detected p1 strains in serum samples from cattle, goats and rabbits suffering different clinical symptoms, in jiangsu province, china. we sequenced the whole genomes of six p1 strains, which showed 99.4%-100% nucleotide sequence similarity and were closely related to other porcine-origin p1 isolates. the sequences ...	2018	28941198
evidence of porcine circovirus-like virus p1 in piglets with an unusual congenital tremor.	outbreaks of trembling and shaking were reported among pigs at two pig farms in jiangsu province, china. serum and tissue samples tested positive for porcine circovirus-like virus p1 and negative for classical swine fever virus, porcine circovirus type 2, astrovirus and porcine pestivirus using pcr/rt-pcr and immunohistochemical techniques. high p1 viral genome loads were identified in sera, brain and lymph node tissue samples by qpcr. in addition, one of the most notable pathological changes wa ...	2017	29178610
protective efficacy of a dna vaccine encoding capsid protein of porcine circovirus-like virus p1 against porcine circovirus 2 in mice.	the capsid protein is the major immunogenic protein of porcine circovirus 2 (pcv2). the nucleotide sequence of porcine circovirus-like virus p1 shares high homology with open reading frame (orf) 2 of pcv2, and orf1 of p1 encodes its structural protein. mice were vaccinated twice intramuscularly with a plasmid expressing the p1 orf1 protein (pcdna3.1(+)-orf1) at 2-week intervals. all animals vaccinated with pcdna3.1(+)-orf1 developed higher specific anti-p1 antibody levels, and had less pcv2 vire ...	2018	29315776