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development and use of an established cell line of the leafhopper circulifer tenellus to characterize spiroplasma citri-vector interactions.a continuous cell line of embryonic origin from the leafhopper circulifer tenellus, ct 1, was established using a protocol modified from thrips cell culture. the line was used to develop an in vitro model to examine the mode of entry of the plant pathogenic mollicute spiroplasma citri into insect host cells. confluent monolayers were achieved in 5-6 months using a simple medium developed for maintaining established leafhopper cell lines. the newly established ct 1 line, and that of another leafh ...19989709012
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