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accumulation of maize chlorotic dwarf virus proteins in its plant host and leafhopper vector.the genome of maize chlorotic dwarf virus (mcdv; genus waikavirus; family sequiviridae) consists of a monopartite positive-sense rna genome encoding a single large polyprotein. antibodies were produced to his-fusions of three undefined regions of the mcdv polyprotein: the n-terminus of the polyprotein (r78), a region between coat proteins (cps) and the nucleotide-binding site (nbs) (r37), and a region between the nbs and a 3c-like protease (r69). the r78 antibodies react with proteins of 50 kda ...200415246276
effect of environmental conditions and leafhopper gender on maize chlorotic dwarf virus transmission by graminella nigrifrons (homoptera: cicadellidae).to determine the most economical and efficient means to maintain cultures of maize chlorotic dwarf virus (mcdv) and to screen for host plant resistance to mcdv, we evaluated the effects of temperature, light intensity, daylength, atmospheric pressure, and leafhopper gender on the frequency of transmission of mcdv by grarminella nigrifrons forbes (homoptera: cicadellidae). female leafhoppers transmitted at higher frequencies than males under most conditions. in temperature studies, transmission r ...200415279251
identification of quantitative trait loci controlling resistance to maize chlorotic dwarf virus.ineffective screening methods and low levels of disease resistance have hampered genetic analysis of maize (zea mays l.) resistance to disease caused by maize chlorotic dwarf virus (mcdv). progeny from a cross between the highly resistant maize inbred line oh1vi and the susceptible inbred line va35 were evaluated for mcdv symptoms after multiple virus inoculations, using the viral vector graminella nigrifrons. symptom severity scores from three rating dates were used to calculate area under the ...200415551042
virus-independent and common transcriptome responses of leafhopper vectors feeding on maize infected with semi-persistently and persistent propagatively transmitted viruses.insects are the most important epidemiological factors for plant virus disease spread, with >75% of viruses being dependent on insects for transmission to new hosts. the black-faced leafhopper (graminella nigrifrons forbes) transmits two viruses that use different strategies for transmission: maize chlorotic dwarf virus (mcdv) which is semi-persistently transmitted and maize fine streak virus (mfsv) which is persistently and propagatively transmitted. to date, little is known regarding the molec ...201424524215
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