Publications

a new insect-specific flavivirus from northern australia suppresses replication of west nile virus and murray valley encephalitis virus in co-infected mosquito cells.	recent reports of a novel group of flaviviruses that replicate only in mosquitoes and appear to spread through insect populations via vertical transmission have emerged from around the globe. to date, there is no information on the presence or prevalence of these insect-specific flaviviruses (isfs) in australian mosquito species. to assess whether such viruses occur locally, we used reverse transcription-polymerase chain reaction (rt-pcr) and flavivirus universal primers that are specific to the ...	2013	23460804
detection of quang binh virus from mosquitoes in china.	flaviviruses present a wide range of genetic diversity and exhibit diverse host relationships. mosquito-borne flaviviruses have recently been isolated and characterized worldwide. yunnan province of china is one of the richest areas of species diversity and is the center of multi-species evolution in mainland asia, which supports the circulation of numerous arthropod-borne viruses (arboviruses). in a screening program of arboviruses, mosquitoes were collected during the mosquito activity season ...	2014	24342141
phylogenetic characterization of a novel insect-specific flavivirus detected in a culex pool, collected from assam, india.	we report the phylogenetic characterization of a unique flavivirus sequence detected in a wild culex tritaeniorhynchus mosquito pool, collected from the northeast indian state of assam.	2015	25999094
a novel insect-specific flavivirus replicates only in aedes-derived cells and persists at high prevalence in wild aedes vigilax populations in sydney, australia.	to date, insect-specific flaviviruses (isfs) have only been isolated from mosquitoes and increasing evidence suggests that isfs may affect the transmission of pathogenic flaviviruses. to investigate the diversity and prevalence of isfs in australian mosquitoes, samples from various regions were screened for flaviviruses by elisa and rt-pcr. thirty-eight pools of aedes vigilax from sydney in 2007 yielded isolates of a novel flavivirus, named parramatta river virus (parv). sequencing of the viral ...	2015	26519596
the insect-specific palm creek virus modulates west nile virus infection in and transmission by australian mosquitoes.	insect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. these viruses may naturally regulate the transmission of pathogenic vertebrate-infecting arboviruses in co-infected mosquitoes. following the isolation of the first australian insect-specific flavivirus (isf), palm creek virus (pcv), we investigated routes of infection and transmission of this virus in key australian arbovirus vectors and its impact on replication ...	2016	27457250
infectious dnas derived from insect-specific flavivirus genomes enable identification of pre- and post-entry host restrictions in vertebrate cells.	flaviviruses such as west nile virus (wnv), dengue virus and zika virus are mosquito-borne pathogens that cause significant human diseases. a novel group of insect-specific flaviviruses (isfs), which only replicate in mosquitoes, have also been identified. however, little is known about the mechanisms of isf host restriction. we report the generation of infectious cdna from two australian isfs, parramatta river virus (parv) and palm creek virus (pcv). using circular polymerase extension cloning ...	2017	28592864
understanding the role of micrornas in the interaction of aedes aegypti mosquitoes with an insect-specific flavivirus.	the flavivirus genus contains some of the most prevalent vector-borne viruses, such as the dengue, zika and yellow fever viruses that cause devastating diseases in humans. however, the insect-specific clade of flaviviruses is restricted to mosquito hosts, albeit they have retained the general features of the genus, such as genome structure and replication. the interactions between insect-specific flaviviruses (isfs) and their mosquito hosts are largely unknown. pathogenic flaviviruses are known ...	2017	28699859
chimeric viruses of the insect-specific flavivirus palm creek with structural proteins of vertebrate-infecting flaviviruses identify barriers to replication of insect-specific flaviviruses in vertebrate cells.	here we report the generation of novel chimeric flaviviruses, which express the prm and e proteins of either dengue or zika viruses on the genomic backbone of palm creek virus (pcv), an insect-specific flavivirus. the chimeric virus particles were antigenically indistinguishable from their parental prm-e donors, but were unable to infect vertebrate cells. an additional chimera (pcv structural genes in the backbone of west nile virus - wnv/pcv-prme) was also unable to infect vertebrate cells, but ...	2019	31524580