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development of a polymerase chain reaction procedure for detection of chicken and turkey parvoviruses.comparative sequence analysis of six independent chicken and turkey parvovirus nonstructural (ns) genes revealed specific genomic regions with 100% nucleotide sequence identity. a polymerase chain reaction (pcr) assay with primers targeting these conserved genome sequences proved to be highly specific and sensitive to detecting parvoviruses in experimentally infected chickens. in a nationwide survey, a total of 138 field enteric samples from poultry flocks were tested by pcr for parvovirus prese ...200919432008
naturally occurring parvoviral infection in hungarian broiler flocks.the major enteric disease (ed) complex in broiler chickens is runting-stunting syndrome and in turkey broilers is poult enteritis mortality syndrome. viruses from numerous families have been identified in the intestinal tracts of poultry with ed, such as astroviridae, coronaviridae, reoviridae, rotaviridae, and parvoviridae. the objective of the present study was to directly demonstrate the presence of the scarcely known chicken parvovirus (chpv) and turkey parvovirus (tupv) in hungarian flocks ...201121500039
high prevalence of turkey parvovirus in turkey flocks from hungary experiencing enteric disease syndromes.samples collected in 2008 and 2009, from 49 turkey flocks of 6 to 43 days in age and presenting clinical signs of enteric disease and high mortality, were tested by polymerase chain reaction and reverse transcription-polymerase chain reaction for the presence of viruses currently associated with enteric disease (ed) syndromes: astrovirus, reovirus, rotavirus, coronavirus, adenovirus, and parvovirus. turkey astroviruses were found in 83.67% of the cases and turkey astrovirus 2 (tast-2) in 26.53%. ...201122017049
identification and phylogenetic diversity of parvovirus circulating in commercial chicken and turkey flocks in croatia.phylogenetic diversity of parvovirus detected in commercial chicken and turkey flocks is described. nine chicken and six turkey flocks from croatian farms were tested for parvovirus presence. intestinal samples from one turkey and seven chicken flocks were found positive, and were sequenced. natural parvovirus infection was more frequently detected in chickens than in turkeys examined in this study. sequence analysis of 400 nucleotide fragments of the nonstructural gene (ns) showed that our sequ ...201122312994
genetic characterization of parvoviruses circulating in turkey and chicken flocks in poland.between 2008 and 2011, commercial turkey and chicken flocks in poland were examined for the presence of turkey parvovirus (tupv) and chicken parvovirus (chpv). clinical samples (10 individual faecal swabs/flock) from 197 turkey flocks (turkeys aged 1 to 19 weeks) and 45 chicken flocks (chickens aged 3 to 17 weeks) were collected in different regions of the country and tested using a pcr assay that targeted the ns1 gene (3'orf). the prevalence of tupv was 29.4 % in the flocks tested, while chpv i ...201223011307
prevalence of parvoviruses in commercial turkey flocks.turkey parvovirus belongs to the family parvoviridae, subfamily parvovirinae, genus parvovirus. since the initial report on turkey parvovirus in the united states appeared in 1983, there had been no further reports of parvovirus in turkeys until 2008. the aims of our study were to determine the prevalence of parvovirus in commercial turkey flocks using pcr; to determine their genetic relationship to previous strains identified in north america and europe; and to test samples for enteric viruses ...201223397849
discovery of a novel parvovirinae virus, porcine parvovirus 7, by metagenomic sequencing of porcine rectal swabs.parvoviruses are a diverse group of viruses containing some of the smallest known species that are capable of infecting a wide range of animals. metagenomic sequencing of pooled rectal swabs from adult pigs identified a 4103-bp contig consisting of two major open reading frames encoding proteins of 672 and 469 amino acids (aa) in length. blastp analysis of the 672-aa protein found 42.4 % identity to fruit bat (eidolon helvum) parvovirus 2 (ehpv2) and 37.9 % to turkey parvovirus (tupv) tp1-2012/h ...201626995221
a novel rodent chapparvovirus in feces of wild rats.chapparvovirus, a recently determined new genus in the family parvoviridae, can infect many species of animals including bats, chickens, and pigs. here, using viral metagenomics method, we identified a novel chapparvovirus from feces of wild rats and designated it as rat parvovirus 2 (rpv2). the nearly complete genome of rpv2 is 4222-nt long and includes two orfs encoding a 654-aa nonstructural protein 1 (ns1) and a 472-aa capsid protein (vp), respectively. phylogenetic analysis over the amino a ...201627473724
prevalence of parvovirus in minnesota turkeys.poult enteritis syndrome (pes) is characterized by enteritis and decreased body weight gain in growing turkey poults between one d and 7 wk of age. another syndrome called light turkey syndrome (lts) causes a decrease in body weight of adult tom turkeys in minnesota leading to huge economic losses. reovirus, rotavirus, and astrovirus have been found in lts and pes flocks in minnesota. we tested 80 fecal pools collected from four lts flocks and 35 fecal pools from non-lts flocks for the presence ...201727591277
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