Publications

a novel pestivirus associated with deaths in pyrenean chamois (rupicapra pyrenaica pyrenaica).	during investigations into recent population decreases in pyrenean chamois (rupicapra pyrenaica pyrenaica) 21 animals found dead or dying were necropsied. immunohistochemistry revealed the presence of a pestivirus in organs from two of the 21 chamois. from one of these animals a pestivirus was isolated from the spleen, skin and serum. the virus had better growth in ovine than in bovine cells and was neutralized most effectively by an anti-border disease virus (bdv) reference antiserum. using pan ...	2004	15557238
pestivirus exposure in free-living and captive deer in austria.	during the hunting season of 2001-02, blood and spleen samples from 59 red deer (cervus elaphus), 77 roe deer (capreolus capreolus), four fallow deer (dama dama), and five chamois (rupicapra rupicapra) were collected from nine hunting districts (n = 133) and one deer farm (n = 12) in southern austria. sera were tested for antibodies against bovine viral diarrhea virus (bvdv) with an enzyme-linked immunosorbent assay (elisa) and virus neutralization tests against three bvdvs and one border diseas ...	2004	15650102
molecular identification of a new pestivirus associated with increased mortality in the pyrenean chamois (rupicapra pyrenaica pyrenaica) in spain.	pestivirus infection was identified in 16 of 17 chamois during an outbreak of a previously unreported disease in pyrenean chamois (rupicapra pyrenaica pyrenaica) in northeastern spain in 2001-02. by analysis of the 5' noncoding regions of the virus, we assigned it to the border disease virus cluster with pairwise similarity values ranging from 82.1% to 88.1%. it will be important to investigate the association of this pestivirus with disease in pyrenean chamois.	2004	15650103
genetic typing and prevalence of border disease virus (bdv) in small ruminant flocks in spain.	between 2001 and 2002, samples from 1,413 animals in 21 spanish small ruminant flocks, most of them with animals showing clinical signs compatible with border disease (bd), were screened for the presence of pestivirus antigen and antibodies by an indirect peroxidase monolayer assay (ipma) and the virus neutralization test (vnt), respectively. although all flocks harboured seropositive animals, virus could only be isolated from animals in five of the flocks. between 4 and 11 months later all anim ...	2006	16857326
transmission of a pestivirus infection in a population of pyrenean chamois.	outbreaks of a previously unrecorded disease have recently affected pyrenean chamois (rupicapra pyrenaica pyrenaica) populations across the mountain range. a pestivirus was hypothesized to be the cause of this emerging disease and this type of virus can cross the species barrier and be transmitted to or from wildlife. using an epidemiological survey conducted from 1995 to 2004 at orlu, france, we characterized the virus and analyzed its transmission. a phylogenetic analysis of viral sequences an ...	2007	17092662
severe outbreak of disease in the southern chamois (rupicapra pyrenaica) associated with border disease virus infection.	an outbreak of a previously unreported disease affecting southern chamois (rupicapra pyrenaica) in the central pyrenees (ne spain) was recorded in 2001 and 2002. there was a marked temporal distribution, most animals being found between february and june. after the outbreak, the population was found to have decreased by about 42%, most probably due to the disease. we examined 20 affected chamois. clinical manifestations included depression, weakness and movement difficulties in all cases. three ...	2007	17101242
genetic and antigenic typing of border disease virus isolates in sheep from the iberian peninsula.	a selection of 10 pestiviruses isolated from sheep from the iberian peninsula from 2001 to 2004 was characterised at the molecular level. the 5' untranslated region (5'-utr) and n(pro)-coding gene were amplified by the reverse transcription-polymerase chain reaction (rt-pcr) and sequenced directly from purified products. all isolates were also typed antigenically with a panel of monoclonal antibodies (mabs) raised against representative isolates of the four recognised pestivirus species. the gen ...	2007	17126573
epidemiological study of border disease virus infection in southern chamois (rupicapra pyrenaica) after an outbreak of disease in the pyrenees (ne spain).	in 2001 and 2002, an outbreak of a previously unreported disease, associated with a border disease virus (bdv), caused high mortality in the southern chamois (rupicapra pyrenaica) population in the alt pallars-aran national hunting reserve in the catalan pyrenees (ne spain). between 2002 and 2006, sera and/or tissue samples taken from 116 healthy chamois shot during the hunting season, plus 42 from chamois affected by different diseases, were studied. a blocking enzyme-immunosorbent assay (elisa ...	2008	17881162
serologic and virologic investigations into pestivirus infection in wild and domestic ruminants in the pyrenees (ne spain).	an outbreak of disease associated to a border disease virus was described in the southern chamois (rupicapra pyrenaica) in spain in 2002. sera and/or spleen samples from 57 mouflon, 15 red deer, 21 roe deer, 3 fallow deer, 55 sheep, 32 cattle, and 68 goats sharing the chamois habitat were studied. an antibody elisa test yielded an inconclusive result in 2 mouflon and positive results in 5 goat sera. comparative virus neutralization tests were performed on the 2 inconclusive mouflons, 3 of the 5 ...	2009	19084245
border disease virus among chamois, spain.	approximately 3,000 pyrenean chamois (rupicapra pyrenaica pyrenaica) died in northeastern spain during 2005-2007. border disease virus infection was identified by reverse transcription-pcr and sequencing analysis. these results implicate this virus as the primary cause of death, similar to findings in the previous epizootic in 2001.	2009	19239761
experimental infection of pigs with border disease virus isolated from pyrenean chamois (rupicapra pyrenaica).	between 2001 and 2007, several outbreaks of disease associated with border disease virus (bdv) infection were reported in the central pyrenees (northeast spain) and were associated with a major reduction in chamois (rupicapra pyrenaica) populations. at the same time, wild boars (sus scrofa) from the same area were found to be seropositive to this pestivirus, without showing clinical signs. the present study examines the susceptibility of domestic swine and the course of the infection with a bdv ...	2010	20453208
complete genomic sequence of a border disease virus isolated from pyrenean chamois.	this report describes the full-length genome sequence of the pestivirus strain h2121 which was recently isolated from pyrenean chamois and typed as border disease virus (bdv) genotype 4. comparison with full-length genomic sequences of the approved pestivirus species bovine viral diarrhea virus-1 (bvdv-1), bvdv-2, bdv, and classical swine fever virus, the tentative species represented by strain giraffe-1, as well as the atypical pestivirus strain th/04_khonkaen confirmed that the chamois pestivi ...	2010	20638945
haematology and serum chemistry of pyrenean chamois (rupicapra pyrenaica) naturally infected with a border disease virus.	in 2005 and 2006 an outbreak of disease associated with border disease virus (bdv) infection caused high mortality in the pyrenean chamois (rupicapra pyrenaica) in the catalan pyrenees (ne spain). the aim of this study was to determine values for different haematological and serum biochemical analytes in 32 free-ranging pyrenean chamois affected by the disease and to compare them with those obtained from healthy chamois. in the affected chamois red blood cell counts, haemoglobin concentrations, ...	2011	20656309
epidemiology of pestivirus infection in wild ungulates of the french south alps.	inter-species transmission is often incriminated in the epidemiology of pestivirus diseases. the purpose of this study was to investigate the prevalence of pestivirus in some mountain wild ungulates and to determine their role in pestivirus transmission, as mountain pastures are a place where cohabitations between wild and domestic ungulates are particularly high. between 2003 and 2007, a longitudinal epidemiological study was carried out on hunted ungulates in the french hautes-alpes department ...	2011	20709472
border disease virus shedding and detection in naturally infected pyrenean chamois (rupicapra pyrenaica).	pyrenean chamois (rupicapra pyrenaica) populations of the central and eastern pyrenees have been affected by severe outbreaks associated with border disease virus (bdv) since 2001. eight pyrenean chamois (7 males and 1 female) from 1 to 8 years of age with clinical signs consistent with bdv infection were studied. at necropsy, whole blood, tissue samples (skin, brain, prescapular lymph node, thyroid gland, lung, liver, spleen, kidney, small intestine, bone marrow, and testicle), urine, and nasal ...	2010	20807933
retrospective study of pestivirus infection in pyrenean chamois (rupicapra pyrenaica) and other ungulates in the pyrenees (ne spain).	in 2001 a new pestivirus (family flaviviridae) was associated with an outbreak of a previously unreported disease in pyrenean chamois (rupicapra pyrenaica) in the pyrenees (ne spain). molecular characterization assigned this virus to the border disease virus (bdv) cluster, bdv-4 genotype. a retrospective study was performed in archived sera and spleen of 74 pyrenean chamois and in archived sera of 28 mouflon (ovis ammon), 56 red deer (cervus elaphus), 43 roe deer (capreolus capreolus) and 29 fal ...	2010	20961710
experimental infection of lambs with border disease virus isolated from a pyrenean chamois.		2010	21257444
experimental infection with chamois border disease virus causes long-lasting viraemia and disease in pyrenean chamois (rupicapra pyrenaica).	since 2001, severe outbreaks of disease associated with border disease virus (bdv) infection have been reported in the pyrenean chamois. the disease is characterized by variable degrees of cachexia, alopecia and neurological manifestations prior to death. the aim of this study was to investigate this disease under experimental conditions. to assess viral virulence, humoral immune response, dissemination and probable routes of transmission, seven chamois (five seronegative and two seropositive to ...	2011	21775580
polymorphisms at mhc class ii drb1 exon 2 locus in pyrenean chamois (rupicapra pyrenaica pyrenaica).	chamois (rupicapra spp.) are mountain ungulates from southern and central europe and the near east. a newly reported border disease virus (bdv) has affected the easternmost populations of pyrenean chamois, leading to a dramatic population decrease that may drive to genetic variability loss. the major histocompatibility complex (mhc) is a sensitive marker for genetic variation of populations: polymorphism on the mhc genes is affected both by pathogens and population dynamics and it is ecologicall ...	2012	22425496
surveillance of border disease in wild ungulates and an outbreak in pyrenean chamois (rupicapra pyrenaica pyrenaica) in andorra.	the principality of andorra is surrounded by areas in which pyrenean chamois (rupicapra pyrenaica pyrenaica) populations were severely affected by infection with border disease virus (bdv) which caused disease outbreaks between 2001 and 2009. nevertheless, the andorran chamois populations were not affected during this period. in light of the severe impact of bdv on several of the neighboring pyrenean chamois populations, we monitored local andorran populations in an effort to detect pestivirus a ...	2012	23060503
two different epidemiological scenarios of border disease in the populations of pyrenean chamois (rupicapra p. pyrenaica) after the first disease outbreaks.	since 2001 several outbreaks of a new disease associated with border disease virus (bdv) infection have caused important declines in pyrenean chamois (rupicapra pyrenaica) populations in the pyrenees. the goal of this study was to analyze the post-outbreak bdv epidemiology in the first two areas affected by disease with the aim to establish if the infection has become endemic. we also investigated if bdv infected wild and domestic ruminants sharing habitat with chamois. unexpectedly, we found di ...	2012	23251417
experimental infection of pregnant pyrenean chamois (rupicapra pyrenaica) with border disease virus subtype 4.	border disease virus (bdv) causes high mortality in pyrenean chamois (rupicapra pyrenaica) on the french and spanish sides of the pyrenees mountains. we investigated the pathology induced by bdv in pregnant chamois via experimental infection. three females were inoculated during the second third of pregnancy with a bdv-4 subgroup strain isolated from a wild pyrenean chamois during an acute epizootic. a fourth pregnant chamois and one nonpregnant ewe were kept as negative controls. animals were m ...	2013	23307372
the two sides of border disease in pyrenean chamois (rupicapra pyrenaica): silent persistence and population collapse.	in 2001, border disease virus (bdv) was identified as the cause of a previously unreported disease in pyrenean chamois (rupicapra pyrenaica) in spain. since then, the disease has caused a dramatic decrease, and in some cases collapse, of chamois populations and has expanded to nearly the entire distribution area in the pyrenees. chamois bdv was characterized as bdv-4 genotype and experimental studies confirmed that it was the primary agent of the disease. the infection has become endemic in the ...	2015	26050575
border disease virus: an exceptional driver of chamois populations among other threats.	though it is accepted that emerging infectious diseases are a threat to planet biodiversity, little information exists about their role as drivers of species extinction. populations are also affected by natural catastrophes and other pathogens, making it difficult to estimate the particular impact of emerging infectious diseases. border disease virus genogroup 4 (bdv-4) caused a previously unreported decrease in populations of pyrenean chamois (rupicapra pyrenaica pyrenaica) in spain. using a po ...	2015	26733943
pestivirus in alpine wild ruminants and sympatric livestock from the cantabrian mountains, spain.	bovine viral diarrhoea virus (bvdv) and border disease virus (bdv) were investigated at the wildlife-livestock interface in the distribution area of chamois in the cantabrian mountains, north-western spain. from 2010 to 2014, sera from sympatric wild (n=167) and domestic (n=272) ruminants were analysed for pestivirus antibodies by celisa, virus neutralisation test (vnt) and for the presence of pestiviral rna using a reverse transcription (rt)-pcr. results showed a higher seroprevalence in cattle ...	2016	27083871
the european hare (lepus europaeus) as a potential wild reservoir for ruminant pestiviruses.	ruminant pestiviruses cause important economic losses in livestock and the epidemiological role of free-ranging sympatric wildlife is of special interest for the implementation of pestivirus eradication plans. moreover, the emergence of high mortality outbreaks of pestivirus in pyrenean chamois (rupicapra pyrenaica pyrenaica) since 2001 in the border between spain and france has increased the value of knowing the hosts that role pestivirus infection. in the present study, pestivirus infection wa ...	2016	27544252
evidence of circulation of the novel border disease virus genotype 8 in chamois.	evidence of association between the novel putative border disease virus genotype 8 (bdv-8) and fatal disease in an alpine chamois (rupicapra rupicapra) is reported. diagnostically, we also demonstrated, as already previously reported, the failure of bdv-specific primers (pdb1 and pdb2) to detect bdv-8.	2017	27761745
spatial and temporal phylogeny of border disease virus in pyrenean chamois (rupicapra p. pyrenaica).	border disease virus (bdv) affects a wide range of ruminants worldwide, mainly domestic sheep and goat. since 2001 several outbreaks of disease associated to bdv infection have been described in pyrenean chamois (rupicapra pyrenaica pyrenaica) in spain, france and andorra. in order to reconstruct the most probable places of origin and pathways of dispersion of bdv among pyrenean chamois, a phylogenetic analysis of 95 bdv 5'untranslated sequences has been performed on chamois and domestic ungulat ...	2016	28033381