Publications

the haemagglutinin-neuraminidase glycoprotein of the porcine paramyxovirus lpmv: comparison with other paramyxoviruses revealed the closest relationship to simian virus 5 and mumps virus.	the complete nucleotide sequence of the haemagglutinin-neuraminidase (hn) gene of the porcine paramyxovirus lpmv, was determined from cdna derived from viral genomic rna. the gene was 1906 nucleotides long including a putative gene end and poly a signal. one long open reading frame was found encoding a protein of 576 amino acids with a calculated molecular weight of 63,324. the protein contains four potential n-glycosylation sites and a major hydrophobic region near the n-terminal, suggesting a ...	1992	1731697
seromonitoring in small laboratory animal colonies. a five year survey: 1984-1988.	from 1984 to 1988 one thousand serologic investigations of laboratory animal colonies originating from 10 different european countries were performed. the most prevalent infections in mouse stocks were caused by mouse hepatitis virus (mhv), minute virus of mice (mvm), theiler's encephalomyelitis virus (temv), reovirus type 3 (reo3), sendaivirus, and pneumonia virus of mice (pvm). in mice no infections with lymphocytic choriomeningitis virus (lcm), polyomavirus, mouse adenovirus, and k-virus were ...	1990	2321435
lack of cross-reaction between myelin basic proteins and putative demyelinating virus envelope proteins.	no cross-reaction could be detected between purified myelin basic proteins (mbp) from mouse, rat or human origins and envelope proteins of viruses suspected of inducing demyelinating processes. in the experimental model using theiler's murine encephalomyelitis virus, competition radioimmunoassay failed to detect any cross-reaction between mbp and vp1, vp2 and vp3 envelope antigens. in the human situation, antibodies against sv5 and measles viruses, both etiologically linked with multiple scleros ...	1989	2476671
rescue of a cytopathic paramyxovirus from peripheral blood leukocytes in subacute sclerosing panencephalitis.	an infectious, cytopathic paramyxovirus was rescued from the peripheral blood leukocytes of a patient with subacute sclerosing panencephalitis (sspe) by cocultivation procedures. cells infected with the virus (1) demonstrated polykaryocytosis, (2) contained cytoplasmic inclusion bodies of paramyxoviral nucleocapsids, (3) hemadsorbed monkey erythrocytes, and (4) reacted with fluorescein-conjugated antisera to measles virus. on the basis of these observations, the virus was initially believed to b ...	1981	7229439
np:p and np:v interactions of the paramyxovirus simian virus 5 examined using a novel protein:protein capture assay.	using recombinant proteins extracted from mammalian cells, in a novel protein:protein binding assay, direct interaction of the nucleoprotein (np) of simian virus 5 with the phosphoprotein (p) and v protein (v) was demonstrated. the amount of np bound by v was found to be significantly less than that bound by p. furthermore, preabsorption of np with p removed the fraction of np that could be bound by v, but preabsorption of np with v did not remove all the np that could be bound by p. these resul ...	1996	8862406
analysis of the fusion protein gene of the porcine rubulavirus lpmv: comparative analysis of paramyxovirus f proteins.	complementary dna clones representing the fusion (f) protein gene of the porcine rubulavirus lpmv were isolated and sequenced. the f gene was found to be 1,845 nucleotides long containing one long open reading frame capable of encoding a protein of 541 amino acids. the cleavage motif for f0 into f1 and f2 is his-arg-lys-lys-arg. a sequence comparison and a phylogenetic analysis was performed in order to identify possible functional domains of paramyxovirus fusion proteins and also to classify th ...	1997	9208455
isolation of a cytopathogenic virus from a case of porcine reproductive and respiratory syndrome (prrs) and its characterization as parainfluenza virus type 2.	from a lung of a fetus of a breeding sow showing prrs-like symptoms a viral agent could be isolated. it was characterized as an enveloped, hemagglutinating rna virus. ultrastructural examination of purified virus revealed paramyxovirus-like pleomorphic virions of approx. 200 nm in diameter. the helical nucleocapsids were about 18 nm in diameter. the virus was found to be antigenically related to simian virus 5 (sv5) a prototype strain of parainfluenza virus type 2, but not to bovine respiratory ...	1998	9856104
regulation of fusion activity by the cytoplasmic domain of a paramyxovirus f protein.	ser virus is a member of the family paramyxoviridae, genus rubulavirus, which has been isolated from pigs. it is very closely related to sv5 virus serologically, in protein profile, and in nucleotide sequence. however, unlike sv5, ser induces minimal syncytium formation in infected cv-1 or bhk cells. fluorescence transfer experiments between labeled erythrocytes and infected mdbk cells revealed that ser also induces hemifusion and pore formation with reduced efficiency. the virion polypeptide pr ...	2002	12359434
activation of fusion by the ser virus f protein: a low-ph-dependent paramyxovirus entry process.	ser virus, a paramyxovirus closely related to simian virus 5, induces no syncytium formation. the ser virus f protein has a long cytoplasmic tail (ct), and truncation or mutations of the ct result in enhanced syncytium formation (s. seth, a. vincent, and r. w. compans, j. virol. 77:167-178, 2003; s. tong, m. li, a. vincent, r. w. compans, e. fritsch, r. beier, c. klenk, m. ohuchi, and h.-d. klenk, virology 301:322-333, 2002). we hypothesized that the presence of the long ct serves to stabilize t ...	2003	12743308
mutations in multiple domains activate paramyxovirus f protein-induced fusion.	ser virus, a paramyxovirus that is closely related to simian virus 5 (sv5), is unusual in that it fails to induce syncytium formation. the ser virus f protein has an unusually long cytoplasmic tail (ct), and it was previously observed that truncations or specific mutations of this domain result in enhanced syncytium formation. in addition to the long ct, the ser f protein has nine amino acid differences from the f protein of sv5. we previously observed only a partial suppression of fusion in a c ...	2004	15280460
relationships and host range of human, canine, simian and porcine isolates of simian virus 5 (parainfluenza virus 5).	sequence comparison of the v/p and f genes of 13 human, canine, porcine and simian isolates of simian virus 5 (sv5) revealed a surprising lack of sequence variation at both the nucleotide and amino acid levels (0-3%), even though the viruses were isolated over 30 years and originated from countries around the world. furthermore, there were no clear distinguishing amino acid or nucleotide differences among the isolates that correlated completely with the species from which they were isolated. in ...	2004	15448364
analysis of the ph requirement for membrane fusion of different isolates of the paramyxovirus parainfluenza virus 5.	paramyxoviruses enter cells by fusing their envelopes with the plasma membrane, a process that occurs at neutral ph. recently, it has been found that there is an exception to this dogma in that a porcine isolate of the paramyxovirus parainfluenza virus 5 (piv5), known as ser, requires a low-ph step for fusion (s. seth, a. vincent, and r. w. compans, j. virol. 77: 6520-6527, 2003). as a low-ph activation mechanism for fusion would greatly facilitate biophysical studies of paramyxovirus-mediated m ...	2006	16501116
a quantitative method for analyzing establishing-efficiency of persistent viral infection.	a quantitative method for analyzing establishing-efficiency of persistent infection was devised. the efficiency of hpiv2 ca and sv5 t1 strains was found to be high, that is, 0.1 approximately 0.3 (an efficiency of 1.0 indicates that 100% of the virus-infected cells became persistently infected). the efficiency of the sv5 wr strain was also high, approximately 0.1, though the virus had no ability to immediately establish a steady state of persistent infection in whole cell-culture systems. at abo ...	2009	19457166
comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (w3a), parainfluenza virus type 3, and respiratory syncytial virus.	parainfluenza virus 5 (piv5) infects a wide range of animals including dogs, pigs, cats, and humans; however, its association with disease in humans remains controversial. in contrast to parainfluenza virus 3 (piv3) or respiratory syncytial virus (rsv), piv5 is remarkably non-cytopathic in monolayer cultures of immortalized epithelial cells. to compare the cytopathology produced by these viruses in a relevant human tissue, we infected an in vitro model of human ciliated airway epithelium and mea ...	2011	21986028