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isolation and characterization of a new insect flavivirus from aedes albopictus and aedes flavopictus mosquitoes in japan.we isolated a new flavivirus from aedes albopictus mosquito and a related species in japan. the virus, designated aedes flavivirus (aefv), only replicated in a mosquito cell line and produced a mild cytopathic effect. the aefv genome was positive-sense, single-stranded rna, 11,064 nucleotides in length and contained a single open reading frame encoding a polyprotein of 3341 amino acids with 5' and 3' untranslated regions (utrs) of 96 and 945 nucleotides, respectively. genetic and phylogenetic an ...200919580982
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