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detection of mycobacterium avium ssp. paratuberculosis in ileocaecal lymph nodes collected from elderly slaughter cows using a semi-nested is900 polymerase chain reaction.the aim of this study was to investigate the occurrence of subclinical mycobacterium avium spp. paratuberculosis (map) infections at slaughter by testing ileocaecal lymph nodes with a semi-nested is900 pcr. tissue samples were available within the framework of a parallel study investigating bse-susceptibility factors in members of bse-cohorts in the german federal state of lower saxony. ileocaecal lymph nodes were collected over a 2-year sampling period from 99 slaughter cattle of a mean age of ...201121775077
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