Publications

molecular characterization and immunogenicity of neutralization-sensitive babesia bigemina merozoite surface proteins.	monoclonal antibodies binding to the surface of live mexico isolate babesia bigemina merozoites have defined 4 parasite-encoded surface antigens (36, 45, 55, and 58 kda) that are potential targets for immune-mediated neutralization of merozoites. in this study, we have characterized the post-translational modification, antigenic polymorphism, and immunogenicity of these 4 proteins. monoclonal antibody immunoaffinity-purified 36- and 55-kda polypeptides were identical in gel electrophoresis to im ...	1991	1944418
development of a recombinant anaplasma marginale dna probe.	an anaplasma marginale dna probe has been developed by using an improved method for the isolation of genomic dna. purified genomic a. marginale dna from the st. croix isolate was partially digested with sau 3a1 into fragments (greater than or equal to 5.0 kb). the restriction fragments were cloned using standard techniques in the pbr322 vector and used to transform e. coli (dh5) host cells. the recombinant a. marginale dna library was screened by the colony lifting procedure. colonies containing ...	1990	2260276
monoclonal antibody to a conserved epitope on proteins encoded by babesia bigemina and present on the surface of intact infected erythrocytes.	to define babesia bigemina-specific antigens on the surface of infected erythrocytes, monoclonal antibodies (mabs) were identified by live-cell immunofluorescence. as determined by live-cell immunofluorescence, two mabs made to the mexico strain reacted with the mexico strain and three kenya strains, while three mabs made to the kenya-ngong strain reacted with the kenya strains but not the mexico strain. binding of mab 44.18 (made to the mexico strain) to a strain-common epitope was confirmed by ...	1995	7543884
identification of babesia bigemina infected erythrocyte surface antigens containing epitopes conserved among strains.	the presence of previously uncharacterized antigens (new antigens) on the surface of intact erythrocytes infected with three strains of babesia bigemina from kenya and one each from puerto rico, mexico, st. croix, and texcoco-mexico was demonstrated by indirect immunofluorescent antibody (ifa) reactions. these antigens were not strain specific because antibodies in bovine immune serum to either the mexico or kenya isolates reacted with all seven strains tested. homologous and heterologous immune ...	1994	8208585
babesia bigemina: identification of b cell epitopes associated with parasitized erythrocytes.	rhoptries are involved in host cell invasion and rhoptry polypeptides, including the babesia bigemina rhoptry-associated protein-1 (rap-1), are targets for protective immune responses. polyclonal antisera produced against isolated rhoptries is directed predominantly against rap-1 and reacts with both the merozoite and the membrane of parasitized erythrocytes. to determine whether these b cell epitopes associated with the parasitized erythrocyte are derived from rap-1 or, alternatively, from prev ...	1995	8542990
molecular basis for variable expression of merozoite surface antigen gp45 among american isolates of babesia bigemina.	immunization with the merozoite surface glycoprotein gp45 induces protection against challenge using the homologous babesia bigemina strain. however, gp45 b-cell epitopes are highly polymorphic among b. bigemina strains isolated from different geographical locations within north and south america. the molecular basis for this polymorphism was investigated using the jg-29 biological clone of a mexico strain of b. bigemina and comparison with the puerto rico, st. croix, and texcoco strains. the mo ...	2001	11349043