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infection and co-infection rates of anaplasma phagocytophilum variants, babesia spp., borrelia burgdorferi, and the rickettsial endosymbiont in ixodes scapularis (acari: ixodidae) from sites in indiana, maine, pennsylvania, and wisconsin.in total, 394 questing adult blacklegged ticks, ixodes scapularis say (acari: ixodidae), collected at four sites were analyzed by polymerase chain reaction (pcr) for five microbial species: anaplasma phagocytophilum, babesia microti, babesia odocoilei, borrelia burgdorferi, and the rickettsial i. scapularis endosymbiont. identities of genetic variants of a. phagocytophilum were determined by sequencing a portion of the 16s dna. in 55% of infected ticks (193/351), a single agent was detected. in ...200818402145
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