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identification and purification of immunogenic proteins from nonliving promastigote polyvalent leishmania vaccine (leishvacin ).immunogenic proteins from nonliving promastigote polyvalent leishmania vaccine against american tegumentary leishmaniasis (leishvacin ), produced by biobr s (biochemistry of brazil ), montes claros, state of minas gerais, brazil, were identified and purified by polyacrylamide electrophoresis gel and electroelution. c57bl/10 mice were vaccinated with proteins with estimated molecular weights of 42, 46, 63, 66, 73, 87, 97, and 160kda in three doses of 30 g of each protein at 15-day intervals combi ...200312806454
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