Publications

development of a recombinant anaplasma marginale dna probe.	an anaplasma marginale dna probe has been developed by using an improved method for the isolation of genomic dna. purified genomic a. marginale dna from the st. croix isolate was partially digested with sau 3a1 into fragments (greater than or equal to 5.0 kb). the restriction fragments were cloned using standard techniques in the pbr322 vector and used to transform e. coli (dh5) host cells. the recombinant a. marginale dna library was screened by the colony lifting procedure. colonies containing ...	1990	2260276
babesia bigemina: identification of b cell epitopes associated with parasitized erythrocytes.	rhoptries are involved in host cell invasion and rhoptry polypeptides, including the babesia bigemina rhoptry-associated protein-1 (rap-1), are targets for protective immune responses. polyclonal antisera produced against isolated rhoptries is directed predominantly against rap-1 and reacts with both the merozoite and the membrane of parasitized erythrocytes. to determine whether these b cell epitopes associated with the parasitized erythrocyte are derived from rap-1 or, alternatively, from prev ...	1995	8542990