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comparison of diagnostic methods to detect piroplasms in asymptomatic cattle.this study was carried out to compare different diagnostic techniques to reveal the presence of piroplasms in asymptomatic cattle kept at pasture. nineteen blood samples were collected from animals of two different areas of emilia romagna region of italy and processed for microscopic observation, pcr, serological test (ifat) for babesia bovis and babesia bigemina antibodies and in vitro cultivation. the cultures were performed on both bovine and ovine erythrocytes. seventeen blood smears (89%) w ...201221846583
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