Publications

virulence of vibrio parahaemolyticus isolated from cultured small abalone, haliotis diversicolor supertexta, with withering syndrome.	outbreaks of mass mortality among cultured small abalone haliotis diversicolor supertexta with withering syndrome occurred in may and september 1998 in kao-hsiung, taiwan. bacterial strains ch-1 and b4 were isolated from the haemolymph of the moribund small abalone using tryptic soy agar supplemented with 3% nacl and/or thiosulphate citrate bile salt sucrose agar. these two strains were characterized and identified as vibrio parahaemolyticus on the basis of various biochemical tests. the b4 stra ...	2000	11123551
semen cryopreservation of small abalone (haliotis diversicolor supertexa).	methods for cryopreserving spermatozoa and maximizing fertilization rate in taiwan small abalone, haliotis diversicolor supertexa, were developed. the gametes (spermatozoa and eggs) of small abalone were viable 3 h post-spawning, with fertilization, and development rate decreasing with time. a minimum of 10(2) cell/ml sperm concentration and a contact time of 2 min between gametes is recommended for artificial insemination of small abalone eggs. eight cryoprotectants, dimethyl sulfoxide (dmso), ...	2002	12374126
shell of abalone haliotis diversicolor supertexta can eliminate waterborne zinc biokinetically.	dietary and nondietary accumulation of waterborne zinc (zn) by shell of abalone haliotis diversicolor supertexta was studied to determine if abalone shell can accumulate and eliminate zn biokinetically. shell of h. diversicolor supertexta rapidly accumulated zn at microgram per gram concentrations during a 7-d uptake period for dietary and nondietary exposure to 1 microgml(-1) zn seawater. depuration half-lives were 7.22 and 15.40 d for dietary and nondietary exposure, respectively. the uptake r ...	2004	14592739
the immune response of taiwan abalone haliotis diversicolor supertexta and its susceptibility to vibrio parahaemolyticus at different salinity levels.	addition of nacl at 2.5% to 3.5% to tryptic soy broth (tsb) significantly increased the growth of vibrio parahaemolyticus. taiwan abalone haliotis diversicolor supertexta held in 30 per thousand seawater were injected with v. parahaemolyticus grown in tsb containing nacl at 0.5, 1.5, 2.5, 3.5 and 4.5% at a dose of 1.6 x 10(5)colony-forming units (cfu) abalone(-1). after 48 h, the cumulative mortality was significantly higher for the abalone challenged with v. parahaemolyticus grown in 2.5% than ...	2004	15123299
effect of ammonia on the immune response of taiwan abalone haliotis diversicolor supertexta and its susceptibility to vibrio parahaemolyticus.	taiwan abalone haliotis diversicolor supertexta held in 30 parts/per thousand seawater and 26 degrees c were injected with tsb-grown vibrio parahaemolyticus (1.6 x 10(5)cfu abalone(-1)), and then placed in water containing different concentrations of ammonia-n (un-ionized plus ionized ammonia) at 0.01 mg l(-1) (control), 1.12, 3.22, 5.24 and 10.18 mg l(-1). mortality of abalone increased directly with ambient ammonia-n concentration. after 12 h, the mortality of v. parahaemolyticus-injected abal ...	2004	15276599
change in water temperature on the immune response of taiwan abalone haliotis diversicolor supertexta and its susceptibility to vibrio parahaemolyticus.	taiwan abalones, haliotis diversicolor supertexta, held in 30 parts/per thousand seawater at 28 degrees c, were injected with tsb-grown vibrio parahaemolyticus (1.6x10(5) cfu abalone(-1)) and then transferred to 20, 24, 28 and 32 degrees c. all abalones transferred to 32 degrees c died by 72 h. the mortality of v. parahaemolyticus-injected abalone held at 20 and 24 degrees c was significantly lower over 24-96 h, compared to animals held at 28 and 32 degrees c. in a separate experiment designed t ...	2004	15276603
effect of nitrite on immune response of taiwan abalone haliotis diversicolor supertexta and its susceptibility to vibrio parahaemolyticus.	taiwan abalones haliotis diversicolor supertexta held in 30% per thousand seawater and 26 degrees c were injected with tryptic soy broth (tsb)-grown vibrioparahaemolyticus (1.6 x 10(5) cfu [colony-forming units] abalone(-1)), and then placed in water containing different concentrations of nitrite-n (nitrite as nitrogen): 0.01 mg l(-1) (control), 1.05, 3.04, 5.10 and 10.06 mg l(-1). mortality of the abalones increased in direct parallel to ambient nitrite-n concentration. over 12 to 48 h, the mor ...	2004	15460860
herpes-like virus infection causing mortality of cultured abalone haliotis diversicolor supertexta in taiwan.	a herpes-like virus is demonstrated for the first time to be associated with high mortality rates in maricultured abalone haliotis diversicolor supertexta in taiwan. histopathology of moribund abalone indicated that the nerve system was the primary target tissue. the lesions were characterised by tissue necrosis accompanied with infiltration of haemocytes. electron microscopic examination demonstrated viral particles within the degenerated cerebral ganglion cells. the viruses were hexagonal, app ...	2005	16042040