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mosquitoes of the anopheles maculipennis group (diptera: culicidae) in romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence.mosquitoes of the anopheles maculipennis group were collected in five districts of romania (constant,a, giurgiu, ilfov, mehedint,i and suceava) between march 2000 and june 2003. two hundred and ninety-seven specimens were identified by molecular methods. nuclear rdna its2 sequences of 178 specimens were compared with genbank sequences for nine known palaearctic species of the group, and 119 specimens were identified using an its2 pcr-rflp assay developed during the study. five genetically distin ...200415541192
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