Publications

genomic characterization of wild-type measles viruses that circulated in different states in brazil during the 1997 measles epidemic.	despite the marked reduction in the incidence of measles in brazil, a measles epidemic occurred in this country in 1997. the measles cases observed during this epidemic began to reappear in large numbers in são paulo, and spread to rio de janeiro and other brazilian states. in the present study molecular biology techniques were used for the detection and genomic characterization of measles viruses from clinical samples such as urine and nasopharyngeal secretions collected in the states of rio de ...	2001	11241461
molecular strategies for identifying human papillomavirus infection in routinely processed samples: focus on paraffin sections.	to optimize the protocols of human papillomavirus (hpv) detection in clinical samples, we used polymerase chain reaction (pcr)-based techniques in paraffin-embedded tissue sections and compared the results with those obtained with pcr and hybrid capture ii (hc2) performed in liquid-based cytology (lbc) preservation medium.	2005	16205192
surveillance of eight respiratory viruses in clinical samples of pediatric patients in southeast brazil.	detection of the eight most common respiratory viruses: human respiratory syncytial virus (hrsv), influenza virus a and b (ia and ib), parainfluenza viruses 1, 2 and 3 (hpiv1, 2 and 3), adenovirus (ad) and human metapneumovirus (hmpv), in order to establish the etiology of acute respiratory infections (aris) and the epidemiology of these viruses in young children seen at hospital universitário, universidade de são paulo, in são paulo, brazil, during 2003.	2007	17940688
variant isolates of human metapneumovirus subgroup b genotype 1 in campinas, brazil.	human metapneumovirus (hmpv) is a paramyxovirus associated with respiratory illness. the genotypes of hmpv isolates in brazil have not been well characterized.	2008	18178521
comparison of genotypes between environmental and clinical isolates of cryptococcus neoformans var. grubii based on microsatellite patterns.	we applied multilocus microsatellite typing (mlmt) method to investigate the genetic relation between cryptococcus neoformans var. grubii clinical and environmental isolates in são paulo, brazil. this mlmt method includes three functional gene sequences of c. neoformans var. grubii, which are dispersed on three chromosomes. in all, 89 strains (36 clinical and 53 environmental isolates) were analyzed. of 36 clinical strains, 20 belonged to a major type of mlmt-13 (55.6%). they were mainly isolate ...	2010	19680781
the detection of in vivo and in vitro hiv type 1 b/f profiles in brazil using a real-time pcr assay for five hiv type 1 genomic regions.	we sought to determine the frequency and profile of hiv-1 bf recombinants in vitro and in vivo. laboratory hiv-1 strains from subtypes b and f were cocultured and evaluated. clinical samples from the city of santos, brazil, where the first hiv-1 b/f circulating recombinant forms (crf) were described, were also assessed. five real-time pcr assays were developed to equally amplify subtypes b and f, and subtype-specific probes were developed and optimized. to validate the pcr systems, clinical samp ...	2010	20707640