Publications

brazilian purpuric fever identified in a new region of brazil. the brazilian purpuric fever study group.	brazilian purpuric fever (bpf) is a life-threatening pediatric infection that is preceded by conjunctivitis and caused by haemophilus influenzae biogroup aegyptius. bpf was first recognized during 1984 in the state of sao paulo. bpf was not reported in brazil outside of sao paulo and the neighboring state of parana until december 1989, when children with bpf were identified in the state of mato grosso. by april 1990, 10 children with confirmed bpf were identified from six widely separated cities ...	1992	1588153
[epidemiological study of brazilian purpuric fever. epidemic in a locality of são paulo state (brazil), 1986].	a case control model was used in the study of an outbreak of brazilian purpuric fever bpf which occurred in serrana, s. paulo state, brazil, in 1986. three hypotheses were raised: 1--purulent conjunctivitis is associated with bpf; 2--a cluster effect occurs in bpf; 3--respiratory symptoms may be a variation of the clinical picture of the disease. numerical values were attributed to different findings, as follows: fever = 5; diarrhea and/or vomiting = 1; haemorrhagic findings = 3; thrombocytopeni ...	1991	1820627
brazilian purpuric fever--mato grosso, brazil.	brazilian purpuric fever (bpf) is a life-threatening pediatric infection that is preceded by conjunctivitis and caused by a specific strain of haemophilus influenzae biogroup aegyptius (bpf clone). bpf was recognized during 1984 in the state of sao paulo, brazil, when 10 children in a town of 20,000 persons died of an acute febrile illness associated with purpura and vascular collapse. until december 1989, no cases of bpf had been reported outside of sao paulo and the neighboring state of parana ...	1990	2123287
rrna gene restriction patterns of haemophilus influenzae biogroup aegyptius strains associated with brazilian purpuric fever.	the rrna gene restriction patterns of 92 isolates of haemophilus influenzae biogroup aegyptius, associated with conjunctivitis or brazilian purpuric fever in the state of são paulo, brazil, were studied with 16 + 23s rrna from escherichia coli as a probe. all strains were classified into 15 patterns. isolates from brazilian purpuric fever cases were seen only in patterns 3 (most frequently) and 4 (rarely), whereas isolates from conjunctivitis were found in all 15 patterns. the study demonstrated ...	1988	2459153
[meningitis. 6,957 cases confined at the "emilio ribas" hospital (são paulo) during the 1958-1967 decade. occurrence, etiology and mortality].		1970	4393708
biotypes and serotypes of haemophilus influenzae from patients with meningitis in the city of são paulo, brazil.	a total of 1,094 haemophilus influenzae isolates from cerebrospinal fluid were examined by biochemical and serological means. most of them belonged to biotype i (70.9%) and to serotype b (99.4%). the relationship of biotypes i and ii to the ages of the patients was shown to be significant (p < 0.001).	1993	8458978
antimicrobial resistance patterns of haemophilus influenzae isolated from patients with meningitis in são paulo, brazil.	from 1989 to 1995, a total of 391 haemophilus influenzae isolates were recovered from the cerebrospinal fluid (csf) of hospitalized patients in são paulo, brazil. the majority of strains were isolated from infants aged less than 5 years. strains belonging to biotype i (64.7%), biotype ii (34.5%) and biotype iv (0.76%) were detected. ninety-nine percent of these strains were serotype b. minimal inhibitory concentration (mic) was determined for ampicillin, chloramphenicol and ceftriaxone. the ss-l ...	2000	10719380
acute otitis media in brazilian children: analysis of microbiology and antimicrobial susceptibility.	between 1990 and 1995, a total of 300 children, ages 2 months to 5 years, received diagnoses of acute otitis media (aom) in a hospital emergency room in são paulo, brazil, and were recruited for this study. the investigation was undertaken, first, to identify microorganisms and antimicrobial susceptibilities of pathogens from aom in brazilian children; next, to ascertain, by comparison, whether the isolates of streptococcus pneumoniae have the same serotypes as those included in the new conjugat ...	2001	11465826
epidemiology of bacterial meningitis among children in brazil, 1997-1998.	to document the incidence and the descriptive epidemiology of bacterial meningitis among individuals under age 20 in a geographically defined region in brazil during the two-year period immediately preceding the introduction of haemophilus influenzae type b (hib) vaccines into the national immunization program of brazil.	2001	11486147
an epidemiological study of haemophilus influenzae at a brazilian day care center.	day care centers are a relatively new phenomenon in brazil that bring together large numbers of young children susceptible to contagious diseases. haemophilus influenzae (hi) is an important infection in the age range of those attending day care centers. in the present study, the carriage rate of haemophilus influenzae was identified in 38 day care attendees age 6 to 37 months, and 23 staff members, at a day care center in ribeirão preto-são paulo, in 1997. to identify the carriers, two nasophar ...	2001	11779452
antimicrobial resistance among invasive haemophilus influenzae strains: results of a brazilian study carried out from 1996 through 2000.	a total of 1712 strains of haemophilus influenzae isolated from patients with invasive diseases were obtained from ten brazilian states from 1996 to 2000. beta-lactamase production was assessed and the minimum inhibitory concentrations (mic) of ampicillin, chloramphenicol, ceftriaxone and rifampin were determined using a method for broth microdilution of haemophilus test medium. the prevalence of strains producing beta-lactamase ranged from 6.6 to 57.7%, with an overall prevalence of 18.4%. high ...	2002	12426628
antimicrobial resistance of streptococcus pneumoniae and haemophilus influenzae in sao paulo, brazil from 1996 to 2000.	this study was undertaken to assess the in vitro activity of several antimicrobial agents against brazilian isolates of streptococcus pneumoniae and haemophilus influenzae from 1996 to 2000. the antibiotics used were penicillin, amoxicillin/clavulanic acid (a/c), ampicillin, amoxicillin, cefaclor, cefdinir, cefixime, cefprozil, ceftriaxone, cefuroxime, azithromycin, clarithromycin, erythromycin, ciprofloxacin, levofloxacin, ofloxacin, chloramphenicol, clindamycin, doxycycline and trimethoprim/su ...	2004	15081084
oropharyngeal colonization by haemophilus influenzae in healthy children from taubaté (são paulo), prior to the haemophilus influenzae type b vaccination program in brazil.	haemophilus influenzae is one of the most important bacterial agents of otitis and sinusitis. h. influenzae type b (hib) is one of the main causes of meningitis, pneumonia, and septicemia in nonvaccinated children under 6 years of age. the aims of this study were to determine the prevalence of h. influenzae and hib oropharyngeal colonization prior to the onset of the hib vaccination program in brazil in previously healthy children and to assess the susceptibility profile of this microorganism to ...	2004	15543393
emergence and disappearance of a virulent clone of haemophilus influenzae biogroup aegyptius, cause of brazilian purpuric fever.	summary: in 1984, children presented to the emergency department of a hospital in the small town of promissão, são paulo state, brazil, with an acute febrile illness that rapidly progressed to death. local clinicians and public health officials recognized that these children had an unusual illness, which led to outbreak investigations conducted by brazilian health officials in collaboration with the u.s. centers for disease control and prevention. the studies that followed are an excellent examp ...	2008	18854482
[epidemiological profile of haemophilus influenzae b meningitis in regional health board of piracicaba--são paulo-brazil].	the objective of the present study was to characterize the population affected by hib meningitis from 1992 to 2001, in the regional health board of piracicaba (são paulo-brazil) in terms of variables regarding demographics and the health-disease process. data were collected from disease notification reports, and arranged using the sinan data program. it was observed there was a peak in the incidence of meningitis in 1994 and a peak of deaths in 1999, before the vaccine had been introduced. the m ...	2010	20964039
incorporation of real-time pcr into routine public health surveillance of culture negative bacterial meningitis in s+úo paulo, brazil.	real-time (rt)-pcr increases diagnostic yield for bacterial meningitis and is ideal for incorporation into routine surveillance in a developing country. we validated a multiplex rt-pcr assay for streptococcus pneumoniae, neisseria meningitidis, and haemophilus influenzae in brazil. risk factors for being culture-negative, rt-pcr positive were determined. the sensitivity of rt-pcr in cerebrospinal fluid (csf) was 100% (95% confidence limits, 96.0%-100%) for n. meningitidis, 97.8% (85.5%-99.9%) fo ...	2011	21731621
changes in serotype distribution of haemophilus influenzae meningitis isolates identified through laboratory-based surveillance following routine childhood vaccination against h. influenzae type b in brazil.	following routine childhood vaccination against haemophilus influenzae type b (hib) disease in brazil in 1999, passive laboratory surveillance reported increasing numbers of non-b serotypes and nontypeable h. influenzae (nthi) from meningitis cases. to characterize this increase, we analyzed data on 3910 h. influenzae isolated from cerebrospinal fluid or blood from meningitis cases that were sent to the national reference laboratory for serotyping from 1990 to 2008. hib accounted for 98% of h. i ...	2011	21945960
use of cerebrospinal fluid and serum samples impregnated on ftatm elute filter paper for the diagnosis of infections caused by neisseria meningitidis, streptococcus pneumoniae and haemophilus influenzae.	the lack of information regarding the burden of acute bacterial meningitis in latin america leads to a reduction in the estimated incidence rates of the disease, and impairs public health decisions on the use and follow-up of preventive interventions, particularly, the evaluation of existing vaccination policies. the use of the real-time pcr in diagnostic routine procedures has resulted in a substantial increase in confirmed bacterial meningitis cases. however, in resource-poor countries, these ...	2017	28235065