Publications

detection of circulant tumor necrosis factor-alpha, soluble tumor necrosis factor p75 and interferon-gamma in brazilian patients with dengue fever and dengue hemorrhagic fever.	pro-inflammatory cytokines are believed to play an important role in the pathogenesis of dengue infection. this study reports cytokine levels in a total of 54 patients examined in recife, state of pernambuco, brazil. five out of eight patients who had hemorrhagic manifestations presented tumor necrosis factor-alpha (tnf-alpha) levels in sera which were statistically higher than those recorded for controls. in contrast, only one out of 16 patients with mild manifestations had elevated tnf-alpha l ...	2001	11285501
[clinical and epidemiological aspects of the dengue epidemic in recife, pe, 2002].	this paper shows data regarding dengue and hemorrhagic fever of the dengue epidemic in recife in 2002 and the clinical, laboratorial and necropsy results from the 14 patients who died that year. the serotype den-3 was detected in 76.3% of cases. the majority of deaths occurred among men, over 20 years old, on the 11th day of disease, attended in the private hospitals. the average values of the hematocrit and platelets were 40.7% and 56,313 p/mm3, respectively. hepatitis, with high levels of tran ...	2006	16501759
characterization of a dengue patient cohort in recife, brazil.	from 2004 to 2006, 658 patients with suspected dengue virus infections were enrolled in a clinical dengue cohort established in recife, pernambuco, located at the northeastern region of brazil. a total of 2,364 blood samples were collected, and serum, plasma, and cells were cryopreserved. among the suspected cases, 354 (54%) were confirmed as acute denv-3 infection based on reverse transcription-polymerase chain reaction, virus isolation, and elisa-igm. according to who criteria, 29.4% of the po ...	2007	18165535
dengue and dengue hemorrhagic fever in the state of pernambuco, 1995-2006.	in pernambuco, the first dengue cases occurred in 1987. after a seven-year interval without autochthonous cases, a new epidemic occurred in 1995. important aspects of the dengue epidemics during the period 1995-2006 have been analyzed here, using epidemiological, clinical and laboratory data. a total of 378,374 cases were notified, with 612 confirmed cases of dengue hemorrhagic fever and 33 deaths. the mortality rate was 5.4%. the incidence rate increased from 134 to 1,438/100,000 inhabitants, c ...	2013	18200409
[frequency of measles, rubella, dengue and erythema infectiosum among suspected cases of measles and rubella in the state of pernambuco between 2001 and 2004].	this study had the aim of investigating the frequency of measles, rubella, dengue and erythema infectiosum among suspected cases of measles and/or rubella in the state of pernambuco, brazil. a total of 1,161 serum samples collected between 2001 and 2004 were tested for these four viral diseases, using enzyme immunoassays to detect igm antibodies. out of this total, 276 (23.8%) samples were positive for one of the four viral diseases analyzed. there were 196 positive cases (16.9%) for dengue, 38 ...	2008	18853004
seroprevalence and risk factors for dengue infection in socio-economically distinct areas of recife, brazil.	brazil currently accounts for the majority of dengue cases reported in the americas, with co-circulation of denv 1-3. striking variation in the epidemiological pattern of infection within cities has been observed. therefore, investigation of dengue transmission in small areas is important to formulate control strategies. a population-based household survey was performed in three diverse socio-economic and environmental areas of recife, a large urban center of brazil, between 2005 and 2006. dengu ...	2010	19896921
gene expression profiling during early acute febrile stage of dengue infection can predict the disease outcome.	we report the detailed development of biomarkers to predict the clinical outcome under dengue infection. transcriptional signatures from purified peripheral blood mononuclear cells were derived from whole-genome gene-expression microarray data, validated by quantitative pcr and tested in independent samples.	2009	19936257
patient-based dengue virus surveillance in aedes aegypti from recife, brazil.	dengue is currently one of the most important arthropod-borne diseases and may be caused by four different dengue virus serotypes (denv-1 to denv-4), transmitted mainly by aedes aegypti (diptera: culicidae) mosquitoes. with the lack of a dengue vaccine, vector control strategies constitute a crucial mode to prevent or reduce disease transmission. in this context, denv detection in natural ae. aegypti populations may serve as a potential additional tool for early prediction systems of dengue outb ...	2010	20539043
from re-emergence to hyperendemicity: the natural history of the dengue epidemic in brazil.	dengue virus (denv) was reintroduced into brazil in 1986 and by 1995 it had spread throughout the country. in 2007 the number of dengue hemorrhagic fever (dhf) cases more than doubled and a shift in the age distribution was reported. while previously the majority of dhf cases occurred among adults, in 2007 53% of cases occurred in children under 15 years old. the reasons for this shift have not been determined.	2011	21245922
[knowledge, attitude and practice on dengue, the vector and control in an urban community of the northeast region, brazil].	this article presents the results of the study on knowledge, attitude and practice (kap) on dengue in an urban community in northeast of brazil, and the risk situations involved in its transmission. the study was observational and used a semi-structured survey composed of questions about the disease and vector control measures, which was answered by residents of selected households (ic 95%) of the community of santa rosa, in the city of cabo de santo agostinho, pernambuco state. the variables of ...	2011	21503482
description of a prospective 17dd yellow fever vaccine cohort in recife, brazil.	from september 2005 to march 2007, 238 individuals being vaccinated for the first time with the yellow fever (yf) -17dd vaccine were enrolled in a cohort established in recife, brazil. a prospective study indicated that, after immunization, anti-yf immunoglobulin m (igm) and anti-yf igg were present in 70.6% (igm) and 98.3% (igg) of the vaccinated subjects. all vaccinees developed protective immunity, which was detected by the plaque reduction neutralization test (prnt) with a geometric mean tit ...	2011	21976581
construction of the discourse on dengue fever in the media.	the aim of this paper was to understand how the media in the state of pernambuco portrays dengue fever, a disease that is affecting more and more brazilians. taking the explosive epidemic of 2002 as the starting point, we tried to understand the effects of meaning produced by the press in a comparative discourse strategy analysis used in 2002, 2004, 2006 and 2008. we selected 291 articles and notes published in the jornal do commercio (recife-brazil), on the situation of this viral disease in th ...	2012	22450493
designing a vaccination strategy against dengue.	in this work we propose a mathematical approach to estimate the dengue force of infection, the average age of dengue first infection, the optimum age to vaccinate children against dengue in a routine fashion and the optimum age interval to introduce the dengue vaccine in a mass vaccination campaign. the model is based on previously published models for vaccination against other childhood infections, which resulted in actual vaccination programmes in brazil. the model was applied for three areas ...	2012	23011455
sustained reduction of the dengue vector population resulting from an integrated control strategy applied in two brazilian cities.	aedes aegypti has developed evolution-driven adaptations for surviving in the domestic human habitat. several trap models have been designed considering these strategies and tested for monitoring this efficient vector of dengue. here, we report a real-scale evaluation of a system for monitoring and controlling mosquito populations based on egg sampling coupled with geographic information systems technology. the smcp-aedes, a system based on open technology and open data standards, was set up fro ...	2013	23844059
twenty years of hantavirus pulmonary syndrome in brazil: a review of epidemiological and clinical aspects.	hantavirus infection is transmitted to humans by wild rodents and the most common clinical form in brazil is the hantavirus pulmonary syndrome (hps). the first serological evidence of the disease was identified in 1990, in recife, pernambuco state, and later in 1993 in juquitiba, state of são paulo. since then there has been a progressive increase in case notification in all regions of the country. the clinical aspects of the disease in brazil are characterized by a prodromal phase, with nonspec ...	2014	24518622
prospective birth cohort in a hyperendemic dengue area in northeast brazil: methods and preliminary results.	dengue cases have increased in younger age groups in brazil. maternal anti-dengue antibodies can have a protective effect in the first months of life, but their decline can increase the risk of severe dengue. a prospective birth cohort was established in 2011-2012 in the city of recife, pernambuco state, brazil, to determine the incidence of serotype-specific dengue infection and the kinetics of transferred maternal anti-dengue antibodies in the first years of life. this article describes the de ...	2016	26886185
investigation into an outbreak of dengue-like illness in pernambuco, brazil, revealed a cocirculation of zika, chikungunya, and dengue virus type 1.	in april 2015, an outbreak of dengue-like illness occurred in tuparetama, a small city in the northeast region of brazil; this outbreak was characterized by its fast expansion. an investigation was initiated to identify the viral etiologies and advise the health authorities on implementing control measures to contain the outbreak. this is the first report of this outbreak in the northeast, even though a few cases were documented earlier in a neighboring city.plasma samples were obtained from 77 ...	0	27015222
zika in pernambuco: rewriting the first outbreak.	a zika virus epidemic was registered in 2015 in northeast brazil. in the state of pernambuco, thousands of classical cases transpired, and in the following months, neurological disturbances in adults and microcephaly in newborns emerged as complications. after the peak of the epidemic, the official system reported only four cases of zika virus but over 100,000 cases of dengue virus. the vigilance system was unable to retrospectively estimate cases or to issue an alert to officially notified case ...	2016	27812648