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molecular identification of anisakis simplex sensu stricto and anisakis pegreffii (nematoda: anisakidae) from fish and cetacean in japanese waters.parasites morphologically consistent with anisakis simplex sensu lato collected from the coast of japan and western north pacific ocean were examined by pcr-rflp of the its region (its1, 5.8 subunit rrna gene and its2) and mtdna cox1. the rflp patterns of rdna generated by hinfi and hhai showed that 100% of the larvae collected from hokkaido and 94% of adults collected from western north pacific ocean were identified as a. simplex sensu stricto. on the other hand, 97% of the larvae collected fro ...200616942906
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