Publications

[spotted fever in the municipality of pedreira, ps, brazil. serologic survey].	cases of brasilian spotted fever (bsf) have been occurred since 1985, in the rural area of pedreira town, situated 160 km away from the city of são paulo (longitude 46 degrees 54'07", latitude 22 degrees 44'21"). aiming at evaluating the prevalence of brasilian spotted fever in this endemic area, single-serum samples were collected from 473 healthy persons, amongst city-dwellers and the local china industry workers. the obtained samples were tested by indirect immunofluorescence (ifa), in order ...	1996	9026831
primary isolation of spotted fever group rickettsiae from amblyomma cooperi collected from hydrochaeris hydrochaeris in brazil.	this paper reports the first isolation of a spotted fever group rickettsia from an amblyomma cooperi ixodid collected from a capybara (hydrochaeris hydrochaeris) in an endemic area of spotted fever in the county of pedreira, state of são paulo, brazil. isolation was performed in vero cell culture and submitted to immunofluorescence, using antibody from rickettsia rickettsii-positive human serum.	1996	9040845
spotted fever in brazil: a seroepidemiological study and description of clinical cases in an endemic area in the state of são paulo.	during 1985-1995, illnesses clinically and epidemiologically compatible with brazilian spotted fever were identified in 17 patients in the county of pedreira, in the state of são paulo, brazil. spotted-fever group rickettsial infection was confirmed by serology and/or immunostaining of tissues in 10 of these patients. immunostaining confirmed infection in a 37-year-old pregnant patient, although rickettsial antigens were not demonstrable in the tissues of the fetus. a serosurvey was conducted in ...	2001	11693878
rickettsia species infecting amblyomma cooperi ticks from an area in the state of são paulo, brazil, where brazilian spotted fever is endemic.	owing to the potential role of the tick amblyomma cooperi in the enzootic cycle of rickettsia rickettsii, the etiologic agent of brazilian spotted fever (bsf), this study evaluated infection by rickettsia species in a. cooperi ticks collected from an area in brazil where bsf is endemic. among a total of 40 a. cooperi adult ticks collected in an area of bsf endemicity in the state of são paulo, pcr analysis detected dna of rickettsia bellii in 16 ticks (40%), and 3 other ticks (7.5%) were positiv ...	2004	14715737
prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a brazilian spotted fever-endemic area in the state of são paulo, brazil: serologic evidence for infection by rickettsia rickettsii and another spotted fever group rickettsia.	in serum samples obtained from all the healthy humans, horses, dogs, and donkeys present on three farms in the pedreira municipality, an endemic area for brazilian spotted fever, an indirect immunofluorescence assay (ifa) detected antibodies against rickettsia rickettsii in 17 (77.3%) horses, 5 (31.3%) dogs (titers ranging from 64 to 4,048), and none of 4 donkeys or 50 humans. five canine and eight equine sera with high antibody titers to r. rickettsii were also tested by ifa against r. bellii, ...	2004	15238696
rickettsial infection in animals and brazilian spotted fever endemicity.	we compared the rickettsial infection status of amblyomma cajennense ticks, humans, dogs, and horses in both brazilian spotted fever (bsf)-endemic and -nonendemic areas in the state of sao paulo, brazil. most of the horses and few dogs from bsf-endemic areas had serologic titers against rickettsia rickettsii antigens. in contrast, no dogs or horses from bsf-nonendemic areas had serologic titers against r. rickettsii antigens, although they were continually exposed to a. cajennense ticks. all hum ...	2005	15752445
detection of brazilian spotted fever infection by polymerase chain reaction in a patient from the state of são paulo.	brazilian spotted fever (bsf) cases have been increasing in the state of são paulo but no genomic information about local rickettsia isolated from humans has been well documented. we recovered spotted-fever group rickettsiae from a sample of patient blood cultured in vero cells using the shell vial technique. rickettsial dna fragments (glta, ompa, and, ompb genes) were detected, and analysis of the ompb gene base sequences showed identity with the rickettsia rickettsii ompb sequence available in ...	2005	16113868
detection of rickettsia rickettsii and rickettsia sp. in blood clots in 24 patients from different municipalities of the state of sao paulo, brazil.	the authors detected rickettsia genus organisms using shell vial and polymerase chain reaction (pcr)/sequencing analysis in blood clots in patients suspected of having brazilian spotted fever (bsf). dna was detected using pcr with three sets of primers to access the glta, ompa, and ompb genes. sequence analysis was carried out using an automatic sequencer with bioedit software. seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. to date ...	2006	17114719
isolation of rickettsia rickettsii and rickettsia bellii in cell culture from the tick amblyomma aureolatum in brazil.	brazilian spotted fever (bsf) is a highly lethal disease caused by rickettsia rickettsii. in the present study, rickettsial infection was evaluated in 669 amblyomma aureolatum adult ticks collected from naturally infested dogs in taiaçupeba, a bsf-endemic area in the state of são paulo. ten (1.49%) ticks were infected with rickettsia bellii, and 6 (0.89%) ticks were infected with r. rickettsii. both rickettsia species were isolated and established in vero cell cultures. the rickettsia isolates w ...	2006	17114770
surveillance for zoonotic vector-borne infections using sick dogs from southeastern brazil.	for many vector-borne organisms, dogs can be used as sentinels to estimate the risk of human infection. the objective of this study was to use dogs as sentinels for multiple vector-borne organisms in order to evaluate the potential for human infection with these agents in southeastern brazil. blood from 198 sick dogs with clinicopathological abnormalities consistent with tick-borne infections were selected at the são paulo state university veterinary teaching hospital in botucatu and tested for ...	2007	18021025
rickettsial infection in capybaras (hydrochoerus hydrochaeris) from são paulo, brazil: serological evidence for infection by rickettsia bellii and rickettsia parkeri.	in brazil, capybaras (hydrochoerus hydrochaeris) are important hosts for amblyomma ticks, which in turn can transmit rickettsiae to humans and animals. therefore, capybaras are potential sentinels for rickettsial infection.	2007	18320102
brazilian spotted fever in the paediatric age-segment in the state of são paulo, southeastern brazil, 2003-2006.		2009	19374639
clusters of brazilian spotted fever in são paulo state, southeastern brazil. a review of official reports and the scientific literature.		2009	19456810
rickettsia monteiroi sp. nov., infecting the tick amblyomma incisum in brazil.	free-living adult amblyomma incisum ticks were collected in an atlantic rainforest area at intervales state park, state of s+úo paulo, brazil. from an a. incisum specimen, rickettsiae were successfully isolated in vero cell culture by the shell vial technique. rickettsial isolation was confirmed by optical microscopy, transmission electron microscopy, and pcrs targeting portions of the rickettsial genes glta, htra, rrs, and sca1 on infected cells. fragments of 1,089, 457, 1,362, and 443 nucleoti ...	2011	21685169
etiologies of rash and fever illnesses in campinas, brazil.	few population-based studies of infectious etiologies of fever-rash illnesses have been conducted. this study reports on enhanced febrile-rash illness surveillance in campinas, brazil, a setting of low measles and rubella virus transmission.	2011	21954258