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[studies on immunity to babesia gibsoni in dogs immunostimulation by bordetella bronchiseptica].four species of bacteria, corynebacterium anaerobium 578, actinobacillus pleuropneumoniae g-4, mycobacterium bovis bcg, and bordetella bronchiseptica a-2, were injected intravenously into mice (5 weeks old, icr-spf). the clearance of carbon from the blood stream and the weights of the spleen and liver were determined as indicators of res stimulation. mouse footpad reaction was assessed as an indicator of delayed-type hypersensitivity to each species of bacteria. the immuno-stimulative activity o ...19902232161
babesia canis canis, babesia canis vogeli, babesia canis rossi: differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal rna genes.the parasites babesia canis and babesia gibsoni (phylum apicomplexa) are responsible for canine babesiosis throughout the world. babesia canis was previously described as a group of three biologically different subspecies, namely b. canis canis, b. canis vogeli, and b. canis rossi. we report partial sequences of small subunit ribosomal rna gene (ssu-rdna) of each subspecies amplified in vitro with primers derived from a semi-conserved region of the ssu-rdna genes in other babesia species. the po ...199910377990
a shared antigen among babesia species: ribosomal phosphoprotein p0 as a universal babesial vaccine candidate.babesia gibsoni ribosomal phosphoprotein p0 (bgp0) was previously identified as a cross-protective antigen against babesia microti infection in mice. interestingly, the same protein showed considerable antigenicity when tested with serum samples collected from babesia-infected animals. moreover, the polyclonal antibody raised against the recombinant bgp0 (rbgp0) recognized the p0 homologues from other babesia species either by immunoblotting or by immunoscreening. the p0 genes from babesia cabal ...200717823817
in vitro evaluation of the growth inhibitory activities of 15 drugs against babesia gibsoni (aomori strain).the in vitro growth inhibitory activities of 15 drugs against babesia gibsoni were evaluated following establishment of a continuous culture isolate (aomori isolate). the culture was successfully continued in an rpmi-1640 medium supplemented with 20% normal canine serum or fetal bovine serum in a humidified atmosphere containing 5% co2 and 5% o2 at 37 degrees c. we used this isolate to evaluate the growth inhibitory effect of naphthoquinone (atovaquone), aromatic diamidine (diminazene and pentam ...200818771856
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