Publications

development of sandwich elisa for the detection of fowl adenovirus 4 associated with hydropericardium syndrome in experimentally infected chicken.	a sandwich elisa was standardized to detect fowl adenovirus (fav) group i antigen in various tissues, namely liver, spleen, bursa, thymus and kidneys of chicks experimentally infected with fowl adenovirus 4 (fav-4) isolated from cases of inclusion body hepatitis-hydropericardium syndrome (ibh-hps). the assay was found to be more sensitive and more specific in comparison to an agar gel immunodiffusion (agid) test, as it could detect fav antigen below the titer of 20,000 tcid50/ml and below 1.14 m ...	2001	11719988
characterization of fowl adenovirus serotype-4 associated with hydropericardium syndrome in chicken.	the polypeptides of three fowl adenovirus-4 (fav-4) field isolates of hydropericardium syndrome from various geographical areas of the country and the standard fav-1 (celo virus) were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and analysed by protein immunoblotting with polyclonal antibodies to fav-4 and fav-1. protein profile analysis of fav-4 isolates revealed similarity of all the eight polypeptides with molecular weight ranging from 20 to 107 kda but differed from ...	2002	12053912
nucleotide sequence analysis of the l1 loop variable region of hexon gene of fowl adenovirus 4 isolates from india.	three fowl adenovirus 4 (fav4) isolates from chicken and one from quail, all from tamil nadu, india were analyzed. the l1 loop variable region of hexon gene of these isolates was amplified by pcr and sequenced. the nucleotide sequences (442 bp) and deduced amino acid sequences of the four isolates were compared with those of other isolates of fav4. the nucleotide sequences of the four isolates had a 98% homology with other indian isolates and a 96% homology with belgian and russian isolates. the ...	2005	15929401
economically important non-oncogenic immunosuppressive viral diseases of chicken--current status.	immunosuppressive viral diseases threaten the poultry industry by causing heavy mortality and economic loss of production, often as a result of the chickens' increased susceptibility to secondary infections and sub-optimal response to vaccinations. this paper aimed to present an up-to-date review of three specific economically important non-oncogenic immunosuppressive viral diseases of chickens, viz. chicken infectious anaemia (cia), infectious bursal disease (ibd) and hydropericardium syndrome ...	2006	16883664
isolation and identification of a fowl adenovirus from wild black kites (milvus migrans).	a fowl adenovirus was isolated from wild black kites (milvus migrans) that died around kashipur, uttarakhand, india. this virus isolate produced cytopathic effects in chicken embryo liver (cel) cells and reacted with fowl adenovirus 4 (fadv-4) antiserum in agar gel immunodiffusion and immunofluorescence tests. the virus isolate was neutralized by fadv-4 antiserum with a neutralization titer of 800. electron microscopy of infected cel cells showed the presence of hexagonal virion particles measur ...	2010	20090043
recombinant hexon antigen based single serum dilution elisa for rapid serological profiling against fowl adenovirus-4 causing hydropericardium syndrome in chickens.	a recombinant hexon antigen based single serum dilution enzyme linked immunosorbent assay (elisa) was developed to measure the specific antibody in sera of chickens against fowl adenovirus-4 (fadv) causing hydropericardium syndrome (hps). an immunodominant partial hexon gene of 737bp was cloned into prset vector and expressed in escherichia coli strain bl21 de3 plys s. expression was tested by western blot test. the purified recombinant protein antigen was used in coating elisa plate for fadv-4 ...	2014	24983486
molecular cloning, expression and characterization of 100k gene of fowl adenovirus-4 for prevention and control of hydropericardium syndrome.	fowl adenovirus-4 is an infectious agent causing hydropericardium syndrome in chickens. adenovirus are non-enveloped virions having linear, double stranded dna. viral genome codes for few structural and non structural proteins. 100k is an important non-structural viral protein. open reading frame for coding sequence of 100k protein was cloned with oligo histidine tag and expressed in escherichia coli as a fusion protein. nucleotide sequence of the gene revealed that 100k gene of fadv-4 has high ...	2016	26558992
development of dot enzyme linked immuno sorbent assay using recombinant hexon protein for detecting antibodies to hydropericardium syndrome disease virus.	dot enzyme linked immuno sorbent assay (elisa) using recombinant hexon protein of fowl adenovirus 4 (fadv 4) was standardized to detect antibody levels against hydropericardium syndrome disease in chickens. results were compared with the dot-elisa using purified virus protein antigen of fadv 4. in an experimental vaccination of broiler chicken, the antibody levels by dot elisa with recombinant protein and purified virus protein antigen ranged from 2.240±0.09 log10 to 2.0±0.04 during 5th to 8th w ...	2015	26954190
genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in china.	since may 2015, severe outbreaks of hepatitis-hydropericardium syndrome (hhs) associated with infections of fowl aviadenovirus (fadv) have emerged in broiler chickens in several chinese provinces. to identify the genotype and gain a better understanding of the genetic properties of the fadv strains responsible for the recent hhs outbreaks in china, the complete genome sequences of five isolates from outbreaks of hhs in broiler chickens in five provinces were determined. the results demonstrated ...	2016	27876783
characterization of a hypervirulent fowl adenovirus 4 with the novel genotype newly prevalent in china and establishment of reproduction infection model of hydropericardium syndrome in chickens.	severe hydropericardium syndrome (hps) has been present in layers in the northeast of china since june 2015, with mortality rates varying from 30 to 90%. dead layers had severe hydropericardium with pericardial volumes of 5 to 20 ml, as well as inclusion body hepatitis. laboratory investigations led to the isolation of a fowl adenovirus strain, hljfad15, from the liver tissue of dead layers. natural deletions of orf19 and orf27 were found in this clinical strain by complete genome sequencing, wh ...	2017	28339951
the first whole genome sequence and pathogenicity characterization of a fowl adenovirus 4 isolated from ducks associated with inclusion body hepatitis and hydropericardium syndrome.	in june 2015, an infectious disease with high prevalence causing severe hydropericardium syndrome (hps) first appeared in duck farms of northeast china. the disease showed high morbidity of 35% and mortality of 15% in a commercial duck farm with 200,000 45-day-old ducks. one strain of hypervirulent fowl adenovirus serotype 4 was identified and designated as hljdad15. the whole genome of the duck isolate was sequenced and found to contain the same large deletions as a genotype that has become pre ...	2017	28622015
overexpression and characterization of the 100k protein of fowl adenovirus-4 as an antiviral target.	100k is an important scaffolding protein of adenoviruses including fowl adenovirus serotype 4 (fadv-4) that causes inclusion body hepatitis-hydropericardium syndrome (ibh-hps) in poultry. 100k carries out the trimerization of the major capsid hexon protein of the virus for the generation of new virions inside the target host cells. despite its critical role for fadv-4, no structural study, in particular, has been conducted so far. here, the overexpression of soluble 100k protein was successfully ...	2017	28666898
an inactivated novel genotype fowl adenovirus 4 protects chickens against the hydropericardium syndrome that recently emerged in china.	since 2015, china has experienced outbreaks of severe hydropericardium syndrome (hps), associated with a novel genotype and hypervirulent fowl adenovirus serotype 4 (fadv-4) infection, with a prevalence in various provinces of the country. this has resulted in huge economic losses in the poultry industry. the novel fadv-4 showed new genome characters, such as the natural deletion of open reading frame (orf) 19 and orf 27 (1966 bp), and high pathogenicity toward chickens. these are coupled with s ...	2017	28786949