Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| neuroendocrine cell hyperplasia of infancy: an unusual cause of hypoxemia in children. | childhood interstitial lung disease (child) is a heterogeneous group of rare disorders characterized by abnormal imaging findings, impaired gas exchange; and is associated with substantial morbidity and mortality. neuroendocrine cell hyperplasia (nehi) is a unique sub-group, which is more prevalent in infants and children younger than 2 years of age, and typically manifests with chronic tachypnea, retractions, hypoxemia and failure to thrive. nehi insidiously appears in the first year of life, s ... | 2016 | 27629751 |
| epidemiology of pertussis in denmark, 1995 to 2013. | we describe incidence and age distribution of laboratory-confirmed pertussis in denmark from 1995 to 2013. notification has been mandatory since 2007. since 1997, an acellular monocomponent vaccine has been used. the latest epidemic occurred in 2002 with an incidence of 36 per 100,000; since 1995, only six infant deaths have been recorded. the inter-epidemic incidence lies below 10 per 100,000. in 1995, the mean age of confirmed cases was 9.2 years (95% confidence interval (ci): 7.9-10.5; median ... | 2016 | 27632433 |
| a novel high-throughput assay to quantify the vaccine-induced inhibition of bordetella pertussis adhesion to airway epithelia. | pertussis or whooping cough is an acute respiratory illness caused by the gram-negative pathogen bordetella pertussis. despite high vaccination coverage whooping cough is currently re-emerging in many developed countries. although the causes of pertussis resurgence are matter of debate, emerging evidences suggest that acellular vaccines efficiently protect against the hallmark symptoms of pertussis disease but fail to prevent colonization. this presumably impacts on increased risk of bacterial t ... | 2016 | 27633511 |
| complete genome sequence of bordetella pertussis strain va-190 isolated from a vaccinated 10-year-old patient with whooping cough. | the number of cases of pertussis has increased in the united states despite vaccination. we present the genome of an isolate of bordetella pertussis from a vaccinated patient from virginia. the genome was sequenced by long-read methodology and compared to that of a clinical isolate used for laboratory studies, d420. | 2016 | 27634997 |
| complete genome sequences of bordetella pertussis vaccine reference strains 134 and 10536. | vaccine formulations and vaccination programs against whooping cough (pertussis) vary worldwide. here, we report the complete genome sequences of two divergent bordetella pertussis reference strains used in the production of pertussis vaccines. | 2016 | 27635001 |
| lncrnas bcyrn1 promoted the proliferation and migration of rat airway smooth muscle cells in asthma via upregulating the expression of transient receptor potential 1. | long noncoding rnas (lncrnas) played important roles in several biological processes through regulating the expression of protein. however, the function of lncrna bcyrn1 in airway smooth muscle cells (asmcs) has not been reported. | 2016 | 27648131 |
| etiology of influenza-like illnesses from sentinel network practitioners in réunion island, 2011-2012. | in réunion island, despite an influenza surveillance established since 1996 by the sentinel general practitioner's network, little is known about the etiology of influenza like-illness (ili) that differs from influenza viruses in a tropical area. we set up a retrospective study using nasal swabs collected by sentinel gps from ili patients in 2011 and 2012. a total of 250 swabs were randomly selected and analyzed by multiplex reverse transcriptase polymerase chain reaction (rt-pcr) including rese ... | 2016 | 27654509 |
| a new socioeconomic status measure for vaccine research in children using individual housing data: a population-based case-control study. | we recently developed houses, an individual housing-based socioeconomic status (ses) measurement for health disparities research. we assessed whether houses was associated with risk of pertussis and pertussis vaccine up-to-date status in children. | 2016 | 27655468 |
| highlights of the 11th international bordetella symposium: from basic biology to vaccine development. | pertussis is a severe respiratory disease caused by infection with the bacterial pathogen bordetella pertussis the disease affects individuals of all ages but is particularly severe and sometimes fatal in unvaccinated young infants. other bordetella species cause diseases in humans, animals, and birds. scientific, clinical, public health, vaccine company, and regulatory agency experts on these pathogens and diseases gathered in buenos aires, argentina from 5 to 8 april 2016 for the 11th internat ... | 2016 | 27655886 |
| prior exposure to bordetella species as an exclusion criterion in the baboon model of pertussis. | the baboon model of bordetella pertussis infection is the newest and most clinically accurate model of the human disease to date. however, among the 15 experimentally infected baboons in this study, a subset of baboons did not exhibit the expected high bacterial colonization levels or increase in white blood cell count. moreover, cultures of nasopharyngeal wash samples from several baboons suggested b. bronchiseptica coinfection. analysis of serum antibodies recognizing filamentous hemagglutinin ... | 2017 | 27666464 |
| biofilm forming potential and antimicrobial susceptibility of newly emerged western australian bordetella pertussis clinical isolates. | whooping cough caused by bordetella pertussis is increasing in several countries despite high vaccine coverage. one potential reason for the resurgence is the emergence of genetic variants of the bacterium. biofilm formation has recently been associated with the pathogenesis of b. pertussis. biofilm formation of 21 western australian b. pertussis clinical isolates was investigated. all isolates formed thicker biofilms than the reference vaccine strain tohama i while retaining susceptibility to a ... | 2016 | 27669900 |
| effectiveness of pertussis vaccination and duration of immunity. | a resurgence of pertussis cases among both vaccinated and unvaccinated people raises questions about vaccine effectiveness over time. our objective was to study the effectiveness of the pertussis vaccine and characterize the effect of waning immunity and whole-cell vaccine priming. | 2016 | 27672225 |
| study on toxicity reduction and potency induction in whole-cell pertussis vaccine by developing a new optimal inactivation condition processed on bordetella pertussis. | whooping cough is caused by bordetella pertussis, and it remains a public health concern. whole-cell pertussis vaccines have been commonly employed for expanded immunization. there is no doubt of the efficacy of whole cell pertussis vaccine, but it is necessary to improve the vaccine to decrease its toxicity. | 2016 | 27679704 |
| the impact of healthcare visit timing on reported pertussis cough duration: selection bias and disease pattern from reported cases in michigan, usa, 2000-2010. | pertussis is a potentially serious respiratory illness characterized by cough of exceptionally long duration of up to approximately100 days. while macrolide antibiotics are an effective treatment, there is an ongoing debate whether they also shorten the length of cough symptoms. we investigated whether public health surveillance data for pertussis, in which cases are identified at diagnosis, are potentially affected by selection bias and the possible consequences for reported cough duration. | 2016 | 27682251 |
| randomized clinical trial of the safety and immunogenicity of the tdap vaccine in pregnant mexican women. | immunization with the tetanus, diphtheria, and pertussis (tdap) vaccine raises controversies on immunogenicity and possible antibody interference. we performed an experimental, double-blind, parallel group controlled clinical trial to evaluate the safety and immunogenicity of the tdap vaccine in 204 pregnant women and their children and to determine its interference in antibody production. pregnant women 18 to 38 y of age with 12 to 24 weeks gestation, a low obstetric risk, and without serious d ... | 2017 | 27686182 |
| the regulator of g-protein signaling rgs16 promotes insulin secretion and β-cell proliferation in rodent and human islets. | g protein-coupled receptor (gpcr) signaling regulates insulin secretion and pancreatic β cell-proliferation. while much knowledge has been gained regarding how gpcrs are activated in β cells, less is known about the mechanisms controlling their deactivation. in many cell types, termination of gpcr signaling is controlled by the family of regulators of g-protein signaling (rgs). rgs proteins are expressed in most eukaryotic cells and ensure a timely return to the gpcr inactive state upon removal ... | 2016 | 27689011 |
| the pertussis hypothesis: bordetella pertussis colonization in the pathogenesis of alzheimer's disease. | while a number of endogenous risk factors including age and genetics are established for alzheimer's disease (ad), identification of acquired, potentially preventable or treatable causes, remains limited. in this paper, we review three epidemiologic case studies and present extensive biologic, immunologic and anatomic evidence to support a novel hypothesis that bordetella pertussis (bp), the bacterium better known to cause whooping cough, is an important potential cause of ad. cross-cultural doc ... | 2017 | 27692981 |
| structure and specificity of the type vi secretion system clpv-tssc interaction in enteroaggregative escherichia coli. | the type vi secretion system (t6ss) is a versatile machine that delivers toxins into either eukaryotic or bacterial cells. it thus represents a key player in bacterial pathogenesis and inter-bacterial competition. schematically, the t6ss can be viewed as a contractile tail structure anchored to the cell envelope. the contraction of the tail sheath propels the inner tube loaded with effectors towards the target cell. the components of the contracted tail sheath are then recycled by the clpv aaa(+ ... | 2016 | 27698444 |
| effects of sphingosine-1-phosphate receptor 1 phosphorylation in response to fty720 during neuroinflammation. | fingolimod (fty720, gilenya), a sphingosine-1-phosphate receptor (s1pr) modulator, is one of the first-line immunomodulatory therapies for treatment of relapsing-remitting multiple sclerosis (ms). human s1pr1 variants have been reported to have functional heterogeneity in vitro, suggesting that s1pr1 function may influence fty720 efficacy. in this study, we examined the influence of s1pr1 phosphorylation on response to fty720 in neuroinflammation. we found that mice carrying a phosphorylation-de ... | 2016 | 27699272 |
| b cell-derived transforming growth factor-β1 expression limits the induction phase of autoimmune neuroinflammation. | studies in experimental autoimmune encephalomyelitis (eae), a murine model of multiple sclerosis (ms), have shown that regulatory b cells modulate the course of the disease via the production of suppressive cytokines. while data indicate a role for transforming growth factor (tgf)-β1 expression in regulatory b cell functions, this mechanism has not yet been tested in autoimmune neuroinflammation. transgenic mice deficient for tgf-β1 expression in b cells (b-tgf-β1(-/-)) were tested in eae induce ... | 2016 | 27708418 |
| immunological signatures after bordetella pertussis infection demonstrate importance of pulmonary innate immune cells. | effective immunity against bordetella pertussis is currently under discussion following the stacking evidence of pertussis resurgence in the vaccinated population. natural immunity is more effective than vaccine-induced immunity indicating that knowledge on infection-induced responses may contribute to improve vaccination strategies. we applied a systems biology approach comprising microarray, flow cytometry and multiplex immunoassays to unravel the molecular and cellular signatures in unprotect ... | 2016 | 27711188 |
| apparent life-threatening event in infancy. | an apparent life-threatening event (alte) is defined as the combination of clinical presentations such as apnea, marked change in skin and muscle tone, gagging, or choking. it is a frightening event, and it predominantly occurs during infancy at a mean age of 1-3 months. the causes of alte are categorized into problems that are: gastrointestinal (50%), neurological (30%), respiratory (20%), cardiovascular (5%), metabolic and endocrine (2%-5%), or others such as child abuse. up to 50% of altes ar ... | 2016 | 27721838 |
| application of omics technologies for evaluation of antibacterial mechanisms of action of plant-derived products. | in the face of increasing bacterial resistance to antibiotics currently in use, the search for new antimicrobial agents has received a boost in recent years, with natural products playing an important role in this field. in fact, several methods have been proposed to investigate the antibacterial activities of natural products. however, given that the ultimate aim is future therapeutic use as novel drugs, it is extremely necessary to elucidate their modes of action, stating the molecular effects ... | 2016 | 27729901 |
| old disease and new challenges: major obstacles of current strategies in the prevention of pertussis. | universal immunization against bordetella pertussis has partially controlled the burden of the disease and its transmission. however, according to recent data, the epidemiology of this vaccine-preventable disease has changed. now, younger infants, adolescents, and adults are at greater risk of infection. this article has studied the interaction between the various factors involved in the changing epidemiology of pertussis and the major obstacles faced by the current strategies in its prevention. | 2016 | 27729960 |
| bordetella adenylate cyclase toxin interacts with filamentous haemagglutinin to inhibit biofilm formation in vitro. | bordetella pertussis, the causative agent of whooping cough, secretes and releases adenylate cyclase toxin (act), which is a protein bacterial toxin that targets host cells and disarms immune defenses. act binds filamentous haemagglutinin (fha), a surface-displayed adhesin, and until now, the consequences of this interaction were unknown. a b. bronchiseptica mutant lacking act produced more biofilm than the parental strain; leading irie et al. to propose the act-fha interaction could be responsi ... | 2017 | 27731909 |
| the mosaic type iv secretion systems. | escherichia coli and other gram-negative and -positive bacteria employ type iv secretion systems (t4sss) to translocate dna and protein substrates, generally by contact-dependent mechanisms, to other cells. the t4sss functionally encompass two major subfamilies, the conjugation systems and the effector translocators. the conjugation systems are responsible for interbacterial transfer of antibiotic resistance genes, virulence determinants, and genes encoding other traits of potential benefit to t ... | 2016 | 27735785 |
| non-specific immunological effects of selected routine childhood immunisations: systematic review. | to identify and characterise non-specific immunological effects after routine childhood vaccines against bcg, measles, diphtheria, pertussis, and tetanus. | 2016 | 27737830 |
| separation and detection of phosphorylated and nonphosphorylated bvga, a bordetella pertussis response regulator, in vivo and in vitro. | protein phosphorylation plays a central role in signal transduction in bacteria. however, separation and detection of the phosphorylated protein from its nonphosphorylated form remain challenging. here we describe a method to detect phosphorylation of the bordetella pertussis response regulator bvga, which is phosphorylated at an aspartate residue (boulanger et al., 2013). this method is based on the proprietary adduct, phos-tag™, a dinuclear metal complex, which together with zn(2+) or mn(2+), ... | 2013 | 27747260 |
| specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone. | there are various autoimmunogenic antigens (ais) in testicular germ cells (tgcs) recognized as foreign by the body's immune system. however, there is little information of tgc-specific ais being available. the aim of this study is to identify tgc-specific ais. we have previously established that immunization using viable syngeneic tgc can also induce murine experimental autoimmune orchitis (eao) without using any adjuvant. this study is to identify tgc-specific ais by tgc liquid chromatography-t ... | 2016 | 27752123 |
| pertussis toxin exploits host cell signaling pathways induced by meningitis-causing e. coli k1-rs218 and enhances adherence of monocytic thp-1 cells to human cerebral endothelial cells. | pertussis toxin (ptx), the major virulence factor of the whooping cough-causing bacterial pathogen bordetella pertussis, permeabilizes the blood-brain barrier (bbb) in vitro and in vivo. breaking barriers might promote translocation of meningitis-causing bacteria across the bbb, thereby facilitating infection. ptx activates several host cell signaling pathways exploited by the neonatal meningitis-causing escherichia coli k1-rs218 for invasion and translocation across the bbb. here, we investigat ... | 2016 | 27754355 |
| pertussis outbreak among patients and healthcare workers in a provincial dialysis facility in japan. | sixteen pertussis cases in haemodialysis patients and healthcare workers were detected in a 25-bed outpatient haemodialysis facility in japan between october 2013 and april 2014. | 2016 | 27760708 |
| use of a toxin neutralization assay to characterize the serologic response to adenylate cyclase toxin after infection with bordetella pertussis. | adenylate cyclase toxin (act) is an essential virulence factor of bordetella pertussis, and antibodies to act protect against b. pertussis infection in mice. the toxin is therefore a strong candidate antigen for addition to future acellular pertussis vaccines. in order to characterize the functionality of the immunologic response to act after infection, we developed an assay for testing the ability of serum samples from subjects infected with b. pertussis to neutralize act-induced cytotoxicity i ... | 2017 | 27760780 |
| immunogenicity and protective efficacy of recombinant iron superoxide dismutase protein from bordetella pertussis in mice models. | whooping cough (pertussis) is a highly contagious respiratory infection caused by bordetella pertussis. although availability of effective pertussis vaccines reportedly decreases the incidence of the disease, b. pertussis circulation in populations has not been eliminated. thus, it is necessary to find new protein candidates with greater immune protective capacities than the currently available acellular pertussis vaccines. in this study, iron superoxide dismutase (fesod) gene (sodb) was cloned, ... | 2016 | 27761933 |
| noninvasive recognition and biomarkers of early allergic asthma in cats using multivariate statistical analysis of nmr spectra of exhaled breath condensate. | asthma is prevalent in children and cats, and needs means of noninvasive diagnosis. we sought to distinguish noninvasively the differences in 53 cats before and soon after induction of allergic asthma, using nmr spectra of exhaled breath condensate (ebc). statistical pattern recognition was improved considerably by preprocessing the spectra with probabilistic quotient normalization and glog transformation. classification of the 106 preprocessed spectra by principal component analysis and partial ... | 2016 | 27764146 |
| induction of gut regulatory cd39(+) t cells by teriflunomide protects against eae. | to determine whether as an orally delivered treatment, teriflunomide, an inhibitor of the mitochondrial enzyme dihydroorotate dehydrogenase approved to treat relapsing forms of multiple sclerosis, could affect gut-associated lymphoid tissue (galt) immune responses functionally. | 2016 | 27766282 |
| design and synthesis of fluorescent acyclic nucleoside phosphonates as potent inhibitors of bacterial adenylate cyclases. | bordetella pertussis adenylate cyclase toxin (act) and bacillus anthracis edema factor (ef) are key virulence factors with adenylate cyclase (ac) activity that substantially contribute to the pathogenesis of whooping cough and anthrax, respectively. there is an urgent need to develop potent and selective inhibitors of bacterial acs with prospects for the development of potential antibacterial therapeutics and to study their molecular interactions with the target enzymes. novel fluorescent 5-chlo ... | 2016 | 27775243 |
| respiratory microbes present in the nasopharynx of children hospitalised with suspected pulmonary tuberculosis in cape town, south africa. | lower respiratory tract infection in children is increasingly thought to be polymicrobial in origin. children with symptoms suggestive of pulmonary tuberculosis (ptb) may have tuberculosis, other respiratory tract infections or co-infection with mycobacterium tuberculosis and other pathogens. we aimed to identify the presence of potential respiratory pathogens in nasopharyngeal (np) samples from children with suspected ptb. | 2016 | 27776489 |
| predicting the presence of bacterial pathogens in the airways of primary care patients with acute cough. | bacterial testing of all patients who present with acute cough is not feasible in primary care. furthermore, the extent to which easily obtainable clinical information predicts bacterial infection is unknown. we evaluated the diagnostic value of clinical examination and testing for c-reactive protein and procalcitonin for bacterial lower respiratory tract infection. | 2017 | 27777252 |
| neisserial heparin binding antigen (nhba) contributes to the adhesion of neisseria meningitidis to human epithelial cells. | neisserial heparin binding antigen (nhba) is a surface-exposed lipoprotein ubiquitously expressed by neisseria meningitidis strains and an antigen of the bexsero® vaccine. nhba binds heparin through a conserved arg-rich region that is the target of two proteases, the meningococcal nalp and human lactoferrin (hlf). in this work, in vitro studies showed that recombinant nhba protein was able to bind epithelial cells and mutations of the arg-rich tract abrogated this binding. all n-terminal and c-t ... | 2016 | 27780200 |
| essential role of bordetella nadc in a quinolinate salvage pathway for nad biosynthesis. | nicotinamide adenine dinucleotide (nad) is produced via de novo biosynthesis pathways and by salvage or recycling routes. the classical bordetella bacterial species are known to be auxotrophic for nicotinamide or nicotinic acid. this study confirmed that bordetella bronchiseptica, bordetella pertussis and bordetella parapertussis have the recycling/salvage pathway genes pnca and pncb, for use of nicotinamide or nicotinic acid, respectively, for nad synthesis. although these bordetellae lack the ... | 2017 | 27783449 |
| moringin activates wnt canonical pathway by inhibiting gsk3β in a mouse model of experimental autoimmune encephalomyelitis. | aberrant canonical wnt-β-catenin signaling has been reported in multiple sclerosis (ms), although the results are controversial. the present study aimed to examine the role of the wnt-β-catenin pathway in experimental ms and also to test moringin (4-[α-l-rhamnopyranosyloxy]-benzyl isothiocyanate), resulting from exogenous myrosinase hydrolysis of the natural phytochemical glucomoringin 4(α-l-rhamnosyloxy)-benzyl glucosinolate as a modulator of neuroinflammation via the β-catenin-pparγ axis. expe ... | 2016 | 27784989 |
| microorganisms causing community-acquired acute bronchitis: the role of bacterial infection. | although acute bronchitis is quite common, there is relatively limited information regarding the microorganisms that are involved in this illness. | 2016 | 27788254 |
| non-culture methods for detecting infection. | 2016 | 27789929 | |
| identification of active constraints in dynamic flux balance analysis. | this study deals with the calibration of dynamic metabolic flux models that are formulated as the maximization of an objective subject to constraints. two approaches were applied for identifying the constraints from data. in the first approach a minimal active number of limiting constraints is found based on data that are assumed to be bounded within sets whereas, in the second approach, the limiting constraints are found based on parametric sensitivity analysis. the ability of these approaches ... | 2017 | 27790866 |
| complete genome sequences of four different bordetella sp. isolates causing human respiratory infections. | species of the genus bordetella associate with various animal hosts, frequently causing respiratory disease. bordetella pertussis is the primary agent of whooping cough and other bordetella species can cause similar cough illness. here, we report four complete genome sequences from isolates of different bordetella species recovered from human respiratory infections. | 2016 | 27795250 |
| prevalence and seasonal distribution of respiratory viruses during the 2014 - 2015 season in istanbul. | acute respiratory tract infection (arti) is one of the most common infections worldwide, causing significant morbidity and mortality. | 2016 | 27800148 |
| modeling the effects of priming with the whole-cell bordetella pertussis vaccine. | 2016 | 27802457 | |
| modeling the effects of priming with the whole-cell bordetella pertussis vaccine-reply. | 2016 | 27802485 | |
| chronic cough in korean adults: a literature review on common comorbidity. | chronic cough is a significant medical condition with high prevalence and a strong negative impact on the quality of life. cough hypersensitivity is thought to underlie chronic cough, with several environmental and host factors interacting to cause neuronal sensitization and chronicity. comorbid conditions affecting cough reflex pathways, such as upper airway diseases, asthma, and gastroesophageal reflux, play important roles in chronic cough. however, their prevalence may vary in patients livin ... | 2016 | 27803879 |
| identification of a two-component class iib bacteriocin in streptococcus pyogenes by recombinase-based in vivo expression technology. | streptococcus pyogenes is a globally prominent bacterial pathogen that exhibits strict tropism for the human host, yet bacterial factors responsible for the ability of s. pyogenes to compete within this limited biological niche are not well understood. using an engineered recombinase-based in vivo expression technology (rivet) system, we identified an in vivo-induced promoter region upstream of a predicted class iib bacteriocin system in the m18 serotype s. pyogenes strain mgas8232. this promote ... | 2016 | 27808235 |
| perspectives for therapeutic hpv vaccine development. | human papillomavirus (hpv) infections and associated diseases remain a serious burden worldwide. it is now clear that hpv serves as the etiological factor and biologic carcinogen for hpv-associated lesions and cancers. although preventative hpv vaccines are available, these vaccines do not induce strong therapeutic effects against established hpv infections and lesions. these concerns create a critical need for the development of therapeutic strategies, such as vaccines, to treat these existing ... | 2016 | 27809842 |
| draft genome sequence of a novel luteimonas sp. strain from coral mucus, hawai'i. | luteimonas sp. strain jm171 was cultivated from mucus collected around the coral porites lobata the jm171 draft genome of 2,992,353 bp contains 2,672 protein-coding open reading frames, 45 trna coding regions, and encodes a putative globin-coupled diguanylate cyclase, jmgreg. | 2016 | 27811107 |
| identifying children at risk of malignant bordetella pertussis infection. | to identify factors associated with malignant pertussis. | 2017 | 27811532 |
| reduction of pertussis inflammatory pathology by therapeutic treatment with sphingosine-1-phosphate receptor ligands by a pertussis toxin-insensitive mechanism. | recent data have demonstrated the potential of sphingosine 1-phosphate (s1p) receptor (s1pr) agonism in the treatment of infectious diseases. a previous study used a murine model of bordetella pertussis infection to demonstrate that treatment with the s1pr agonist aal-r reduces pulmonary inflammation during infection. in the current study, we showed that this effect is mediated via the s1pr1 on lysm+ (myeloid) cells. signaling via this receptor results in reduced lung inflammation and cellular r ... | 2017 | 27815382 |
| multifunctional and redundant roles of borrelia burgdorferi outer surface proteins in tissue adhesion, colonization, and complement evasion. | borrelia burgdorferi is the causative agent of lyme disease in the u.s., with at least 25,000 cases reported to the cdc each year. b. burgdorferi is thought to enter and exit the bloodstream to achieve rapid dissemination to distal tissue sites during infection. travel through the bloodstream requires evasion of immune surveillance and pathogen clearance in the host, a process at which b. burgdorferi is adept. b. burgdorferi encodes greater than 19 adhesive outer surface proteins many of which h ... | 2016 | 27818662 |
| crystal structure of bacterial haem importer complex in the inward-facing conformation. | pathogenic bacteria remove iron from the haem of host tissues and use it as a catalytic center of many enzymes. haem uptake by pathogenic bacteria is facilitated by the membrane-integrated haem importer, which belongs to the type ii atp-binding cassette (abc) transporter. here we present crystal structures of burkholderia cenocepacia haem importer bhuuv complexed with the periplasmic haem-binding protein bhut and in the absence of bhut. the transmembrane helices of these structures show an inwar ... | 2016 | 27830695 |
| gm-csf-producing th cells in rats sensitive and resistant to experimental autoimmune encephalomyelitis. | given that granulocyte macrophage colony-stimulating factor (gm-csf) is identified as the key factor to endow auto-reactive th cells with the potential to induce neuroinflammation in experimental autoimmune encephalomyelitis (eae) models, the frequency and phenotype of gm-csf-producing (gm-csf+) th cells in draining lymph nodes (dlns) and spinal cord (sc) of albino oxford (ao) and dark agouti (da) rats immunized for eae were examined. the generation of neuroantigen-specific gm-csf+ th lymphocyte ... | 2016 | 27832210 |
| lack of association between viral load and severity of acute bronchiolitis in infants. | to investigate the correlation between respiratory syncytial viral load and length of hospitalization in infants with acute wheezing episodes. | 2016 | 27832233 |
| identification of ybey-protein interactions involved in 16s rrna maturation and stress regulation in escherichia coli. | ybey is part of a core set of rnases in escherichia coli and other bacteria. this highly conserved endoribonuclease has been implicated in several important processes such as 16s rrna 3' end maturation, 70s ribosome quality control, and regulation of mrnas and small noncoding rnas, thereby affecting cellular viability, stress tolerance, and pathogenic and symbiotic behavior of bacteria. thus, ybey likely interacts with numerous protein or rna partners that are involved in various aspects of cell ... | 2016 | 27834201 |
| the importance of persistence. | 2016 | 27838662 | |
| pertussis in young infants throughout the world. | in typical pertussis in young infants, the child will appear deceptively well; he or she will have coryza, sneezing, and a mild cough. there is no fever. this progresses to gagging, gasping, eye bulging, bradycardia, cyanosis, and vomiting. there is leukocytosis with lymphocytosis and apneic episodes. deaths relate to leukocytosis, pulmonary hypertension, and pneumonia. the source of pertussis in young infants is most often a family member with cough illness that is not recognized as pertussis. ... | 2016 | 27838663 |
| assessing the evidence for maternal pertussis immunization: a report from the bill & melinda gates foundation symposium on pertussis infant disease burden in low- and lower-middle-income countries. | implementation of effective interventions has halved maternal and child mortality over the past 2 decades, but less progress has been made in reducing neonatal mortality. almost 45% of under-5 global mortality now occurs in infants <1 month of age, with approximately 86% of neonatal deaths occurring in low- and lower-middle-income countries (lmics). as an estimated 23% of neonatal deaths globally are due to infectious causes, maternal immunization (mi) is one intervention that may reduce mortali ... | 2016 | 27838664 |
| global childhood deaths from pertussis: a historical review. | impact of pertussis vaccines on mortality is a key world health organization indicator, and trends in mortality rates and age distribution can inform maternal immunization strategies. we systematically reviewed studies reporting pertussis mortality rates (pmrs) per million population, identifying 19 eligible studies. during a prevaccine observation period of ≥50 years in high-income countries (hics), pmrs reduced in both infants and 1- to 4-year-olds by >80%, along with improvements in living co ... | 2016 | 27838665 |
| morbidity and mortality due to bordetella pertussis: a significant pathogen in west africa? | in the absence of specific surveillance platforms for pertussis and availability of suitable diagnostics at the hospital level, reliable data that describe morbidity and mortality from pertussis are difficult to obtain in any setting, as is the case in west africa. here, we summarize the available evidence of the burden of pertussis in the region, given historical data, and describe recent and ongoing epidemiological studies that offer opportunities for additional data collection. the available ... | 2016 | 27838666 |
| epidemiology of pertussis among young pakistani infants: a community-based prospective surveillance study. | pertussis remains a cause of morbidity and mortality among young infants. there are limited data on the pertussis disease burden in this age group from low- and lower-middle-income countries, including in south asia. | 2016 | 27838667 |
| incidence of severe and nonsevere pertussis among hiv-exposed and -unexposed zambian infants through 14 weeks of age: results from the southern africa mother infant pertussis study (samips), a longitudinal birth cohort study. | maternal vaccination with tetanus, reduced-dose diphtheria, and acellular pertussis vaccine (tdap) could be an effective way of mitigating the high residual burden of infant morbidity and mortality caused by bordetella pertussis to better inform such interventions, we conducted a burden-of-disease study to determine the incidence of severe and nonsevere pertussis among a population of zambian infants. | 2016 | 27838668 |
| the burden of pertussis hospitalization in hiv-exposed and hiv-unexposed south african infants. | there are limited data on pertussis in african children, including among human immunodeficiency virus (hiv)-exposed infants. we conducted population-based hospital surveillance to determine the incidence and clinical presentation of bordetella pertussis-associated hospitalization in perinatal hiv-exposed and -unexposed infants. | 2016 | 27838669 |
| bordetella pertussis infection in south african hiv-infected and hiv-uninfected mother-infant dyads: a longitudinal cohort study. | there is a paucity of data regarding the burden of bordetella pertussis in african women and young infants, and particularly the impact of maternal human immunodeficiency virus (hiv) infection thereon. we performed a retrospective analysis of respiratory illness samples from longitudinal cohorts of hiv-uninfected and hiv-infected women and their infants to evaluate the burden of pertussis illness in a black-african community. | 2016 | 27838670 |
| comparing the yield of nasopharyngeal swabs, nasal aspirates, and induced sputum for detection of bordetella pertussis in hospitalized infants. | advances in molecular laboratory techniques are changing the landscape of bordetella pertussis illness diagnosis. polymerase chain reaction (pcr) assays have greatly improved the sensitivity detection and the turnaround time to diagnosis compared to culture. moreover, different respiratory specimens, such as flocked nasopharyngeal swabs (npss), nasopharyngeal aspirates (npas), and induced sputum, have been used for b. pertussis detection, although there is limited head-to-head comparison to eval ... | 2016 | 27838671 |
| pertussis-associated pneumonia in infants and children from low- and middle-income countries participating in the perch study. | few data exist describing pertussis epidemiology among infants and children in low- and middle-income countries to guide preventive strategies. | 2016 | 27838672 |
| forecasting epidemiological consequences of maternal immunization. | the increase in the incidence of whooping cough (pertussis) in many countries with high vaccination coverage is alarming. maternal pertussis immunization has been proposed as an effective means of protecting newborns during the interval between birth and the first routine dose. however, there are concerns regarding potential interference between maternal antibodies and the immune response elicited by the routine schedule, with possible long-term population-level effects. | 2016 | 27838674 |
| influence of population demography and immunization history on the impact of an antenatal pertussis program. | antenatal pertussis vaccination is being considered as a means to reduce the burden of infant pertussis in low- and middle-income countries (lmics), but its likely impact in such settings is yet to be quantified. | 2016 | 27838675 |
| modulating endotoxin activity by combinatorial bioengineering of meningococcal lipopolysaccharide. | neisseria meningitidis contains a very potent hexa-acylated lps that is too toxic for therapeutic applications. we used systematic molecular bioengineering of meningococcal lps through deletion of biosynthetic enzymes in combination with induction of lps modifying enzymes to yield a variety of novel lps mutants with changes in both lipid a acylation and phosphorylation. mass spectrometry was used for detailed compositional determination of the lps molecular species, and stimulation of immune cel ... | 2016 | 27841285 |
| modulation of pertussis and adenylate cyclase toxins by sigma factor rpoe in bordetella pertussis. | bordetella pertussis is a human pathogen that can infect the respiratory tract and cause the disease known as whooping cough. b. pertussis uses pertussis toxin (pt) and adenylate cyclase toxin (act) to kill and modulate host cells to allow the pathogen to survive and persist. b. pertussis encodes many uncharacterized transcription factors, and very little is known about their functions. rpoe is a sigma factor which, in other bacteria, responds to oxidative, heat, and other environmental stresses ... | 2017 | 27849178 |
| concentrations of immunoglobulin g antibodies against pertussis toxin does not decrease over a long period of time in japan. | objective adult patients with pertussis rarely show typical symptoms, such as paroxysmal coughing, inspiratory "whoop", or post-tussive vomiting. while a culture is regarded as the gold standard for diagnosis, the sensitivity is very low. therefore, the diagnosis of pertussis in adults in clinical practice is mostly based on single-sample serology using an enzyme-linked immunosorbent assay (elisa) with the pertussis toxin antigen. various cut-off values for the anti-pertussis toxin igg (pt-igg) ... | 2016 | 27853066 |
| evasion of neutrophil extracellular traps by respiratory pathogens. | the release of neutrophil extracellular traps (nets) is a major immune mechanism intended to capture pathogens. these histone- and protease-coated dna structures are released by neutrophils in response to a variety of stimuli, including respiratory pathogens, and have been identified in the airways of patients with respiratory infection, cystic fibrosis, acute lung injury, primary graft dysfunction, and chronic obstructive pulmonary disease. net production has been demonstrated in the lungs of m ... | 2017 | 27854516 |
| hospital costs of bordetella pertussis in new zealand children. | to estimate hospitalisation costs for children with pertussis in new zealand. | 2016 | 27857241 |
| il-17-producing innate and pathogen-specific tissue resident memory γδ t cells expand in the lungs of bordetella pertussis-infected mice. | γδ t cells play a role in protective immunity to infection at mucosal surface, but also mediate pathology in certain autoimmune diseases through innate il-17 production. recent reports have suggested that γδ t cells can have memory analogous to conventional αβ t cells. in this study we have examined the role of γδ t cells in immunity to the respiratory pathogen bordetella pertussis γδ t cells, predominantly vγ4(-)γ1(-) cells, produced il-17 in the lungs as early as 2 h after infection. the bacte ... | 2017 | 27864475 |
| [trends in pertussis mortality and morbidity in colombia, 2002-2012]. | whooping cough is a public health problem that mainly affects children under one year of age with highly lethal outcomes. it is a re-emerging disease, which is preventable by immunization. objective: to analyze mortality and morbidity trends of whooping cough in colombia between 2002 and 2012. materials and methods: we conducted a quantitative descriptive study of deaths by pertussis between 2002 and 2012 using data from death certificates registered by the departamento administrativo nacional d ... | 2016 | 27869387 |
| does breastfeeding protect young infants from pertussis? case-control study and immunologic evaluation. | pertussis infection can be severe in unvaccinated infants. a case-control study was conducted to investigate the potential role of breastfeeding in protecting young, unvaccinated infants from pertussis. | 2017 | 27870812 |
| an anti-tnf-α antibody mimetic to treat ocular inflammation. | infliximab is an antibody that neutralizes tnf-α and is used principally by systemic administration to treat many inflammatory disorders. we prepared the antibody mimetic fab-peg-fab (fpfinfliximab) for direct intravitreal injection to assess whether such formulations have biological activity and potential utility for ocular use. fpfinfliximab was designed to address side effects caused by antibody degradation and the presence of the fc region. surface plasmon resonance analysis indicated that i ... | 2016 | 27874029 |
| epidemiology of pertussis in two ibero-american countries with different vaccination policies: lessons derived from different surveillance systems. | pertussis is a re-emerging disease worldwide despite its high vaccination coverage. european and latin-american countries have used different surveillance and vaccination policies against pertussis. we compared the epidemiology of this disease in two ibero-american countries with different vaccination and surveillance policies. | 2016 | 27876021 |
| a parasite-derived 68-mer peptide ameliorates autoimmune disease in murine models of type 1 diabetes and multiple sclerosis. | helminth parasites secrete molecules that potently modulate the immune responses of their hosts and, therefore, have potential for the treatment of immune-mediated human diseases. fhhdm-1, a 68-mer peptide secreted by the helminth parasite fasciola hepatica, ameliorated disease in two different murine models of autoimmunity, type 1 diabetes and relapsing-remitting immune-mediated demyelination. unexpectedly, fhhdm-1 treatment did not affect the proliferation of auto-antigen specific t cells or t ... | 2016 | 27883079 |
| pertussis in north-central and northwestern regions of algeria. | pertussis outbreaks continue to occur in many countries despite high vaccination coverage. under-diagnosed cases in adolescents and adults may result in increased transmission to infants, who are at risk of severe pertussis. additional measures to protect both groups should be considered. | 2016 | 27886031 |
| genome-wide discovery of putative srnas in paracoccus denitrificans expressed under nitrous oxide emitting conditions. | nitrous oxide (n2o) is a stable, ozone depleting greenhouse gas. emissions of n2o into the atmosphere continue to rise, primarily due to the use of nitrogen-containing fertilizers by soil denitrifying microbes. it is clear more effective mitigation strategies are required to reduce emissions. one way to help develop future mitigation strategies is to address the currently poor understanding of transcriptional regulation of the enzymes used to produce and consume n2o. with this ultimate aim in mi ... | 2016 | 27895629 |
| a t4ss effector targets host cell alpha-enolase contributing to brucella abortus intracellular lifestyle. | brucella abortus, the causative agent of bovine brucellosis, invades and replicates within cells inside a membrane-bound compartment known as the brucella containing vacuole (bcv). after trafficking along the endocytic and secretory pathways, bcvs mature into endoplasmic reticulum-derived compartments permissive for bacterial replication. brucella type iv secretion system (virb) is a major virulence factor essential for the biogenesis of the replicative organelle. upon infection, brucella uses t ... | 2016 | 27900285 |
| co-regulation of iron metabolism and virulence associated functions by iron and xibr, a novel iron binding transcription factor, in the plant pathogen xanthomonas. | abilities of bacterial pathogens to adapt to the iron limitation present in hosts is critical to their virulence. bacterial pathogens have evolved diverse strategies to coordinately regulate iron metabolism and virulence associated functions to maintain iron homeostasis in response to changing iron availability in the environment. in many bacteria the ferric uptake regulator (fur) functions as transcription factor that utilize ferrous form of iron as cofactor to regulate transcription of iron me ... | 2016 | 27902780 |
| bordetella pertussis outer membrane vesicle vaccine confers equal efficacy in mice with milder inflammatory responses compared to a whole-cell vaccine. | the demand for improved pertussis vaccines is urgent due to the resurgence of whooping cough. a deeper understanding of the mode of action of pertussis vaccines is required to achieve this improvement. the vaccine-induced effects of a candidate outer membrane vesicle vaccine (omvpv) and a classical protective but reactogenic whole cell vaccine (wpv) were comprehensively compared in mice. the comparison revealed essential qualitative and quantitative differences with respect to immunogenicity and ... | 2016 | 27905535 |
| seroprevalence of bordetella pertussis specific immunoglobulin g antibody levels among asymptomatic individuals aged 4 to 24 years: a descriptive cross sectional study from sri lanka. | in sri lanka pertussis continues to circulate in the community and cases among adolescents and adults have been reported despite 95% coverage of the four dose pertussis vaccination during early childhood. waning of immunity following natural infection or immunization may contribute to the persistent circulation. an adolescent booster dose is not included in the national immunization schedule of sri lanka, although this is routine practice in many countries. therefore information on immunity to p ... | 2016 | 27905894 |
| crystal structure of chitinase chiw from paenibacillus sp. str. fpu-7 reveals a novel type of bacterial cell-surface-expressed multi-modular enzyme machinery. | the gram-positive bacterium paenibacillus sp. str. fpu-7 effectively hydrolyzes chitin by using a number of chitinases. a unique chitinase with two catalytic domains, chiw, is expressed on the cell surface of this bacterium and has high activity towards various chitins, even crystalline chitin. here, the crystal structure of chiw at 2.1 å resolution is presented and describes how the enzyme degrades chitin on the bacterial cell surface. the crystal structure revealed a unique multi-modular archi ... | 2016 | 27907169 |
| whole-genome sequencing and comparative genomic analysis of bordetella pertussis isolates from the 2007-2008 epidemic in israel. | 2017 | 27914992 | |
| otopathogenic pseudomonas aeruginosa enters and survives inside macrophages. | otitis media (om) is a broad term describing a group of infectious and inflammatory disorders of the middle ear. despite antibiotic therapy, acute om can progress to chronic suppurative otitis media (csom) characterized by ear drum perforation and purulent discharge. pseudomonas aeruginosa is the most common pathogen associated with csom. although, macrophages play an important role in innate immune responses but their role in the pathogenesis of p. aeruginosa-induced csom is not known. the obje ... | 2016 | 27917157 |
| actin activates pseudomonas aeruginosa exoy nucleotidyl cyclase toxin and exoy-like effector domains from martx toxins. | the nucleotidyl cyclase toxin exoy is one of the virulence factors injected by the pseudomonas aeruginosa type iii secretion system into host cells. inside cells, it is activated by an unknown eukaryotic cofactor to synthesize various cyclic nucleotide monophosphates. exoy-like adenylate cyclases are also found in multifunctional-autoprocessing repeats-in-toxin (martx) toxins produced by various gram-negative pathogens. here we demonstrate that filamentous actin (f-actin) is the hitherto unknown ... | 2016 | 27917880 |
| factors influencing the spread of pertussis in households: a prospective study, catalonia and navarre, spain, 2012 to 2013. | we aimed to investigate transmission rates of pertussis in household contacts of cases and factors associated with transmission. a prospective epidemiological study was conducted in 2012 and 2013 to determine the incidence of pertussis among household contacts of reported cases in catalonia and navarre, spain. an epidemiological survey was completed for each case and contact, who were followed for 28 days to determine the source of infection (primary case) and detect the occurrence of secondary ... | 2016 | 27918260 |
| drivers of airborne human-to-human pathogen transmission. | airborne pathogens - either transmitted via aerosol or droplets - include a wide variety of highly infectious and dangerous microbes such as variola virus, measles virus, influenza a viruses, mycobacterium tuberculosis, streptococcus pneumoniae, and bordetella pertussis. emerging zoonotic pathogens, for example, mers coronavirus, avian influenza viruses, coxiella, and francisella, would have pandemic potential were they to acquire efficient human-to-human transmissibility. here, we synthesize in ... | 2017 | 27918958 |
| fulminant ecchymosis as the initial manifestation of antiphospholipid syndrome (aps) triggered by respiratory syncytial virus (rsv) infection: a case report and review of the literature. | we present a unique and informative instance of respiratory syncytial virus (rsv) infection associated with antiphospholipid syndrome (aps), and discuss this case in the context of the literature addressing the immunopathogenesis of aps associated with diverse infections. we describe the case of a 43-year-old man with no significant past medical history who presented with the acute onset of fever, hemoptysis, and extensive bullous, ecchymotic lesions in both lower extremities. punch biopsy of th ... | 2016 | 27920986 |
| fulminant ecchymosis as the initial manifestation of antiphospholipid syndrome (aps) triggered by respiratory syncytial virus (rsv) infection: a case report and review of the literature. | we present a unique and informative instance of respiratory syncytial virus (rsv) infection associated with antiphospholipid syndrome (aps), and discuss this case in the context of the literature addressing the immunopathogenesis of aps associated with diverse infections. we describe the case of a 43-year-old man with no significant past medical history who presented with the acute onset of fever, hemoptysis, and extensive bullous, ecchymotic lesions in both lower extremities. punch biopsy of th ... | 2016 | 27920986 |
| oxygen and bis(3',5')-cyclic dimeric guanosine monophosphate binding control oligomerization state equilibria of diguanylate cyclase-containing globin coupled sensors. | bacteria sense their environment to alter phenotypes, including biofilm formation, to survive changing conditions. heme proteins play important roles in sensing the bacterial gaseous environment and controlling the switch between motile and sessile (biofilm) states. globin coupled sensors (gcs), a family of heme proteins consisting of a globin domain linked by a central domain to an output domain, are often found with diguanylate cyclase output domains that synthesize c-di-gmp, a major regulator ... | 2016 | 27933792 |
| tolerance checkpoint bypass permits emergence of pathogenic t cells to neuromyelitis optica autoantigen aquaporin-4. | aquaporin-4 (aqp4)-specific t cells are expanded in neuromyelitis optica (nmo) patients and exhibit th17 polarization. however, their pathogenic role in cns autoimmune inflammatory disease is unclear. although multiple aqp4 t-cell epitopes have been identified in wt c57bl/6 mice, we observed that neither immunization with those determinants nor transfer of donor t cells targeting them caused cns autoimmune disease in recipient mice. in contrast, robust proliferation was observed following immuni ... | 2016 | 27940915 |
| consensus recommendation for india and bangladesh for the use of pneumococcal vaccine in mass gatherings with special reference to hajj pilgrims. | respiratory tract infections are prevalent among hajj pilgrims with pneumonia being a leading cause of hospitalization. streptococcus pneumoniae is a common pathogen isolated from patients with pneumonia and respiratory tract infections during hajj. there is a significant burden of pneumococcal disease in india, which can be prevented. guidelines for preventive measures and adult immunization have been published in india, but the implementation of the guidelines is low. data from bangladesh are ... | 2016 | 27942192 |