Publications

Mainstreams of horizontal gene exchange in enterobacteria: consideration of the outbreak of enterohemorrhagic E. coli O104:H4 in Germany in 2011.	Escherichia coli O104:H4 caused a severe outbreak in Europe in 2011. The strain TY-2482 sequenced from this outbreak allowed the discovery of its closest relatives but failed to resolve ways in which it originated and evolved. On account of the previous statement, may we expect similar upcoming outbreaks to occur recurrently or spontaneously in the future? The inability to answer these questions shows limitations of the current comparative and evolutionary genomics methods.	2011	22022434
Arcanolysin is a cholesterol-dependent cytolysin of the human pathogen Arcanobacterium haemolyticum.	Arcanobacterium haemolyticum is an emerging human pathogen that causes pharyngitis, wound infections, and a variety of occasional invasive diseases. Since its initial discovery in 1946, this Gram positive organism has been known to have hemolytic activity, yet no hemolysin has been previously reported. A. haemolyticum also displays variable hemolytic activity on laboratory blood agar that is dependent upon which species the blood is derived.	2011	22029628
is escherichia coli urinary tract infection a zoonosis? proof of direct link with production animals and meat.	recently, it has been suggested that the escherichia coli causing urinary tract infection (uti) may come from meat and animals. the purpose was to investigate if a clonal link existed between e. coli from animals, meat and uti patients. twenty-two geographically and temporally matched b2 e. coli from uti patients, community-dwelling humans, broiler chicken meat, pork, and broiler chicken, previously identified to exhibit eight virulence genotypes by microarray-detection of approximately 300 gene ...	2011	22033854
Large-scale preparation and characterization of non-pegylated and pegylated superactive ovine leptin antagonist.	Superactive ovine leptin antagonist (SOLA) was prepared by rational mutagenesis of the ovine leptin antagonist L39A/D40A/F41A mutant prepared previously in our lab by mutating wild type leptin to D23L/L39A/D40A/F41A. SOLA was expressed in Escherichia coli as insoluble inclusion bodies, refolded and purified to homogeneity (as evidenced by SDS-PAGE and analytical gel filtration) by ion-exchange chromatography. The purified protein was mono-pegylated at its N terminus by 20-kDa linear pegylation r ...	2012	22040607
low concentrations of honey reduce biofilm formation, quorum sensing, and virulence in escherichia coli o157:h7.	bacterial biofilms are associated with persistent infections due to their high resistance to antimicrobial agents. hence, controlling pathogenic biofilm formation is important in bacteria-related diseases. honey, at a low concentration of 0.5% (v/v), significantly reduced biofilm formation in enterohemorrhagic escherichia coli o157:h7 without inhibiting the growth of planktonic cells. conversely, this concentration did not inhibit commensal e. coli k-12 biofilm formation. transcriptome analyses ...	2011	22047137
DDX1 Is an RNA-Dependent ATPase Involved in HIV-1 Rev Function and Virus Replication.	The human immunodeficiency virus type 1 (HIV-1) Rev protein is essential for the virus because it promotes nuclear export of alternatively processed mRNAs, and Rev is also linked to translation of viral mRNAs and genome encapsidation. Previously, the human DEAD-box helicase DDX1 was suggested to be involved in Rev functions, but this relationship is not well understood. Biochemical studies of DDX1 and its interactions with Rev and model RNA oligonucleotides were carried out to investigate the mo ...	2012	22051512
dna, protein binding, cytotoxicity, cellular uptake and antibacterial activities of new palladium(ii) complexes of thiosemicarbazone ligands: effects of substitution on biological activity.	the coordination propensities of 4(n,n')-diethylaminosalicylaldehyde-4(n)-substituted thiosemicarbazones (h(2)l(1-4)) were investigated by reacting with an equimolar amount of [pdcl(2)(pph(3))(2)]. the new complexes were characterized by various spectroscopic techniques. the structure determination of the complexes [pd(deasal-tsc)(pph(3))] (1), [pd(deasal-mtsc)(pph(3))] (2) and [pd(deasal-etsc)(pph(3))] (3) by x-ray crystallography showed that ligands are coordinated in a dibasic tridentate ons ...	2011	22051854
interventions to reduce/eliminate escherichia coli o157:h7 in ground beef.	the escherichia coli o157:h7 (e. coli o157:h7) outbreak in the northwestern united states ushered in an era that has dramatically changed the way beef processors in the united states convert live cattle into meat. unprecedented cooperation among the beef processors and massive investment in research by the us government and the beef industry have resulted in an acceptable level of control of e. coli o157:h7 in ground beef. the evidence to support the progress in control of e. coli o157:h7 is the ...	2007	22061399
in vivo comparison of cxa-101 (fr264205) with and without tazobactam versus piperacillin-tazobactam using human simulated exposures against phenotypically diverse gram-negative organisms.	cxa-101 is a novel antipseudomonal cephalosporin with enhanced activity against gram-negative organisms displaying various resistance mechanisms. this study evaluates the efficacy of exposures approximating human percent free time above the mic (%ft > mic) of cxa-101 with or without tazobactam and piperacillin-tazobactam (tzp) against target gram-negative organisms, including those expressing extended-spectrum β-lactamases (esbls). sixteen clinical gram-negative isolates (6 pseudomonas aeruginos ...	2012	22064538
proanthocyanidin-rich extracts from cranberry fruit ( vaccinium macrocarpon ait.) selectively inhibit the growth of human pathogenic fungi candida spp. and cryptococcus neoformans.	cranberry ( vaccinium macrocarpon ) has been shown in clinical studies to reduce infections caused by escherichia coli and other bacteria, and proanthocyanidins are believed to play a role. the ability of cranberry to inhibit the growth of opportunistic human fungal pathogens that cause oral, skin, respiratory, and systemic infections has not been well-studied. fractions from whole cranberry fruit were screened for inhibition of five candida species and cryptococcus neoformans , a causative ...	2011	22066866
split-protein systems: beyond binary protein-protein interactions.	it has been estimated that 650,000 protein-protein interactions exist in the human interactome (stumpf et al., 2008), a subset of all possible macromolecular partnerships that dictate life. thus there is a continued need for the development of sensitive and user-friendly methods for cataloguing biomacromolecules in complex environments and for detecting their interactions, modifications, and cellular location. such methods also allow for establishing differences in the interactome between a norm ...	2011	22070901
Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole.	Tetracycline antibiotics are widely used in human and veterinary medicine; however, residual amounts of these antibiotics in the environment are of concern since they could contribute to selection of resistant bacteria. In this study, tetracycline (TC), chlortetracycline (CTC), doxycycline (DC) and oxytetracycline (OTC) were treated with laccase from the white rot fungus Trametes versicolor in the presence of the redox mediator 1-hydroxybenzotriazole (HBT). High performance liquid chromatography ...	2012	22071243
a novel cold-adapted lipase from sorangium cellulosum strain so0157-2: gene cloning, expression, and enzymatic characterization.	genome sequencing of cellulolytic myxobacterium sorangium cellulosum reveals many open-reading frames (orfs) encoding various degradation enzymes with low sequence similarity to those reported, but none of them has been characterized. in this paper, a predicted lipase gene (lipa) was cloned from s. cellulosum strain so0157-2 and characterized. lipa is 981-bp in size, encoding a polypeptide of 326 amino acids that contains the pentapeptide (ghsmg) and catalytic triad residues (ser114, asp250 and ...	2011	22072918
mapping nanomechanical properties of live cells using multi-harmonic atomic force microscopy.	the nanomechanical properties of living cells, such as their surface elastic response and adhesion, have important roles in cellular processes such as morphogenesis, mechano-transduction, focal adhesion, motility, metastasis and drug delivery. techniques based on quasi-static atomic force microscopy techniques can map these properties, but they lack the spatial and temporal resolution that is needed to observe many of the relevant details. here, we present a dynamic atomic force microscopy metho ...	2011	22081213
A new recombinant human apolipoprotein E mimetic peptide with high-density lipoprotein binding and function enhancing activity.	We generated a novel human apolipoprotein E (apoE)-mimetic peptide, designated EpK. EpK contains an N-terminal cysteine residue, a low-density lipoprotein receptor-binding fragment, a 6 × lysine linker and a lipid-binding fragment. The recombinant peptide was expressed in Escherichia coli, and purified with a chitin bead column followed by a Heparin Sepharose CL-6B column to yield pure peptide. EpK displayed high solubility in aqueous solution at neutral pH and adopted a low content of a-helical ...	2011	22087021
[neuroprotection of a neotype transactivating-brain-derived neurotrophic factor fusion protein with penetrating activity of blood-brain barrier].	to identify the dural activities of neuroprotection and penetrating blood-brain barrier (bbb) for tat-bdnf (transactivating-brain-derived neurotrophic factor) fusion protein to explore an alternative treatment for the injury of central nerve system (cns).	2011	22093740
High diversity and differential persistence of fecal Bacteroidales population spiked into freshwater microcosm.	Bacteroidales markers are promising indicators of fecal pollution and are now widely used in microbial source tracking (MST) studies. However, a thorough understanding of the persistence of Bacteroidales population after being released into environmental waters is lacking. We investigated the persistence of two host specific markers (HF183 and CF193) and temporal change of Bacteroidales population over 14 days in freshwater microcosms seeded with human or bovine feces. The concentrations of HF18 ...	2012	22100053
production, secretion, and cell surface display of recombinant sporosarcina ureae s-layer fusion proteins in bacillus megaterium.	monomolecular crystalline bacterial cell surface layers (s-layers) have broad application potential in nanobiotechnology due to their ability to generate functional supramolecular structures. here, we report that bacillus megaterium is an excellent host organism for the heterologous expression and efficient secretion of hemagglutinin (ha) epitope-tagged versions of the s-layer protein ssla from sporosarcina ureae atcc 13881. three chimeric proteins were constructed, comprising the precursor, c-t ...	2012	22101038
role of incp-1β plasmids pwdl7::rfp and pnb8c in chloroaniline catabolism as determined by genomic and functional analyses.	broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. to gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an incp-1 chloroaniline degradation plasmid, pwdl7::rfp and its close relative pnb8c, as well as the expression pattern, function, and bioaugmentation potential of the putative 3-chloroaniline (3-ca) oxidation genes. based on phylogenetic analysis of backbon ...	2011	22101050
crystallization and preliminary x-ray crystallographic study of the human mst2 sarah domain.	the sarah domain at the c-terminus of human mst2 (residues 436-484) was overproduced and purified using an escherichia coli expression system. the purified domain was crystallized using the hanging-drop vapour-diffusion technique. two crystal forms were obtained. the crystals belonged to space group p2, with unit-cell parameters a = 62.0, b = 119.2, c = 62.0 å, α = 90.0, β = 90.5, γ = 90.0°, or to space group p6(1)22, with unit-cell parameters a = 54.5, b = 54.5, c = 303.1 å. these crystals diff ...	2011	22102242
Profile of Shiga toxin-producing Escherichia coli strains isolated from dogs and cats and genetic relationships with isolates from cattle, meat and humans.	Pets can be reservoirs of Shiga toxin-producing Escherichia coli (STEC) strains. The aim of this study was to examine nine strains belonging to several serotypes (O91:H21, O91:H16, O178:H19, O8:H19, O22:H8, O22:HNT, ONT:H8), previously recovered from cats or dogs. To this end, we assessed a set of additional virulence genes (stx(2) subtype, subAB, ehxA, eae and saa), cytotoxic activity, and genetic relationships with strains isolated from cattle, meat and humans using pulsed-field gel electropho ...	2011	22119188
a potential test system for detecting contaminations by bacterial lipoproteins.	biological specimens are often contaminated with bacteria-derived products such as lps or lipoproteins (lp), which trigger unwanted inflammatory responses in hosts. whereas a contamination by lps can be determined by various test systems, a contamination by lp can as yet not be determined. tlr4 and tlr2 are key components of the lps and the lp receptor complex, respectively. it was tested in this study whether hek293 cell stably transfected with bovine tlr2 have the ability to react to low conce ...	2011	22133281
functional reconstitution of human eukaryotic translation initiation factor 3 (eif3).	protein fate in higher eukaryotes is controlled by three complexes that share conserved architectural elements: the proteasome, cop9 signalosome, and eukaryotic translation initiation factor 3 (eif3). here we reconstitute the 13-subunit human eif3 in escherichia coli, revealing its structural core to be the eight subunits with conserved orthologues in the proteasome lid complex and cop9 signalosome. this structural core in eif3 binds to the small (40s) ribosomal subunit, to translation initiatio ...	2011	22135459
genomic anatomy of escherichia coli o157:h7 outbreaks.	the rapid emergence of escherichia coli o157:h7 from an unknown strain in 1982 to the dominant hemorrhagic e. coli serotype in the united states and the cause of widespread outbreaks of human food-borne illness highlights a need to evaluate critically the extent to which genomic plasticity of this important enteric pathogen contributes to its pathogenic potential and its evolution as well as its adaptation in different ecological niches. aimed at a better understanding of the evolution of the e. ...	2011	22135463
3-cyano-8-methyl-2-oxo-1,4-disubstituted-1,2,5,6,7,8-hexahydroquinolines. synthesis and biological evaluation as antimicrobial and cytotoxic agents.	the synthesis, in vitro antimicrobial and cytotoxic activities of some novel hexahydroquinolines supported with various pharmacophores are described. the results revealed that 18 compounds displayed pronounced activity against staphylococcus aureus and escherichia coli bacteria beside a moderate antifungal activity. compound 25 is the most active candidate with equipotency to ampicillin against s. aureus, e. coli and pseudomonas aeruginosa, together with an obvious antifungal activity. additiona ...	2011	22136527
Sublingual Immunization with M2-Based Vaccine Induces Broad Protective Immunity against Influenza.	The ectodomain of matrix protein 2 (M2e) of influenza A virus is a rationale target antigen candidate for the development of a universal vaccine against influenza as M2e undergoes little sequence variation amongst human influenza A strains. Vaccine-induced M2e-specific antibodies (Abs) have been shown to display significant cross-protective activity in animal models. M2e-based vaccine constructs have been shown to be more protective when administered by the intranasal (i.n.) route than after par ...	2011	22140491
Pre-Clinical Assessment of Novel Multivalent MSP3 Malaria Vaccine Constructs.	MSP3 has been shown to induce protection against malaria in African children. The characterization of a family of Plasmodium falciparum merozoite surface protein 3 (MSP3) antigens sharing a similar structural organization, simultaneously expressed on the merozoite surface and targeted by a cross-reactive network of protective antibodies, is intriguing and offers new perspectives for the development of subunit vaccines against malaria.	2011	22145028
function and structure studies of gh family 31 and 97 α-glycosidases.	a huge number of glycoside hydrolases are classified into the glycoside hydrolase family (gh family) based on their amino-acid sequence similarity. the glycoside hydrolases acting on α-glucosidic linkage are in gh family 4, 13, 15, 31, 63, 97, and 122. this review deals mainly with findings on gh family 31 and 97 enzymes. research on two gh family 31 enzymes is described: clarification of the substrate recognition of escherichia coli α-xylosidase, and glycosynthase derived from schizosaccharomyc ...	2011	22146713
interaction of endothelial progenitor cells expressing cytosine deaminase in tumor tissues and 5-fluorocytosine administration suppresses growth of 5-fluorouracil-sensitive liver cancer in mice.	the drug delivery system to tumors is a critical factor in upregulating the effect of anticancer drugs and reducing adverse events. recent studies indicated selective migration of bone marrow-derived endothelial progenitor cells (epc) into tumor tissues. cytosine deaminase (cd) transforms nontoxic 5-fluorocytosine (5-fc) into the highly toxic 5-fluorouracil (5-fu). we investigated the antitumor effect of a new cd/5-fc system with cd cdna transfected epc for hepatocellular carcinoma (hcc) in mice ...	2011	22151662
Chlorine disinfection of Pseudomonas aeruginosa, total coliforms, Escherichia coli and Enterococcus faecalis: revisiting reclaimed water regulations.	Pathogenic organisms can be transmitted orally through drinking water or through skin and mucosae by both direct and indirect contact, and their presence in water thus has a negative impact on public health. In wastewater treatment plants (WWTP), water is disinfected to inactivate pathogens. The quantification of several microbial indicators in aquatic systems is required to estimate the biological quality of such systems. So far, coliform bacteria have been used as traditional indicators world- ...	2011	22156117
polyclonal antibody against conserved sequences of mce1a protein blocks mtb infection in macrophages.	the pathogenesis of mycobacterium tuberculosis is largely due to its ability to enter and survive within human macrophages. it is suggested that a specific protein namely mammalian cell entry protein is involved in the pathogenesis and the specific gene for this protein mce1a has been identified in several pathogenic organisms such as rickettsia, shigella, escherichia coli, helicobacter, streptomyces, klebsiella, vibrio, neisseria, rhodococcus, nocardioides, saccharopolyspora erthyrae, and pseud ...	2011	22159737
Expression of Multisubunit Proteins in Leishmania tarentolae.	Heterologous gene expression in mammalian cells is the first choice for the production of recombinant proteins when posttranslational modifications affect the biological activity of target proteins. However, the expression efficiency of mammalian cells is relatively low compared to other expression systems, such as Escherichia coli or yeast. Recently, a novel protein expression system based on Leishmania tarentolae, a protozoan parasite of gecko, was developed. This system allows not only easy h ...	2012	22160906
a rapid and highly sensitive protocol for the detection of escherichia coli o157:h7 based on immunochromatography assay combined with the enrichment technique of immunomagnetic nanoparticles.	escherichia coli o157:h7 (e. coli o157:h7) is an important pathogenic bacterium that threatens human health. a rapid, simple, highly sensitive, and specific method for the detection of e. coli o157:h7 is necessary.	2011	22163159
Molecular expression and characterization of a novel protein phosphatase 2A gene from Clonorchis sinensis.	Reversible phosphorylation of proteins is a critical mechanism involved in physiological function of organisms, including Clonorchis sinensis. In the present study, One cDNA clone encoding protein phosphatase 2A (CsPP2A) was isolated from a C. sinensis adult cDNA plasmid library. The open reading frame of the novel gene contains 924 bp and encoded a putative protein of 307 amino acids. A similarity analysis showed high homology with Schistosoma japonicum (76.3%) and Homo sapiens (84.4%), respect ...	2011	22167368
Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: An ethnomedicinal plant.	This study was carried out with an objective to investigate the antibacterial and antifungal potentials of leaves of Cassia fistula Linn. The aim of the study is to assess the antimicrobial activity and to determine the zone of inhibition of extracts on some bacterial and fungal strains. In the present study, the microbial activity of hydroalcohol extracts of leaves of Cassia fistula Linn. (an ethnomedicinal plant) was evaluated for potential antimicrobial activity against medically important ba ...	2011	22171301
Chimeras of Escherichia coli and Mycobacterium tuberculosis Single-Stranded DNA Binding Proteins: Characterization and Function in Escherichia coli.	Single stranded DNA binding proteins (SSBs) are vital for the survival of organisms. Studies on SSBs from the prototype, Escherichia coli (EcoSSB) and, an important human pathogen, Mycobacterium tuberculosis (MtuSSB) had shown that despite significant variations in their quaternary structures, the DNA binding and oligomerization properties of the two are similar. Here, we used the X-ray crystal structure data of the two SSBs to design a series of chimeric proteins (mß1, mß1'ß2, mß1-ß5, mß1-ß6 an ...	2011	22174737
ubiquitin-intein and sumo2-intein fusion systems for enhanced protein production and purification.	although most commonly used for protein production, expression of soluble and functional recombinant protein in escherichia coli is still a major challenge. the development and application of fusion tags that can facilitate protein expression and solubility partly solve this problem, however, under most circumstance, the fusion tags have to be removed by proteases in order to use the proteins. because the tag removal using proteases increases cost and introduces extra purification steps, it rema ...	2011	22178731
lps from neisseria meningitidis is crucial for inducing monocyte- and microparticle-associated tissue factor activity but not for tissue factor expression.	neisseria meningitidis causes sepsis with coagulopathy. the present study evaluated the tissue factor (tf)-inducing capacity of bacterial lps in different presentation forms, i.e. membrane-bound lps versus purified lps, and of non-lps components of n. meningitidis. by using a wild-type n. meningitidis, a mutant n. meningitidis lacking lps (lps-deficient n. meningitidis), purified lps from n. meningitidis and escherichia coli, we measured tf-expression and tf-activity on human monocytes and micro ...	2011	22180561
molecular and physiological characterization of fluoroquinolone resistance in relation to uropathogenicity among escherichia coli isolates isolated from wenyu river, china.	increasing antibacterial resistance and pathogenicity in the environment is of growing concern due to its potential human risk. in the present study, 236 escherichia coli isolates were collected from wenyu river in china on drugless (48 isolates) and quinolone-containing plates (189 isolates). their minimum inhibitory concentrations (mics) were determined ranging from 0.125μgml(-1) to 128μgml(-1). mutation points related to fluoroquinolone resistance were observed at s83 to l and d87 to n or y i ...	2011	22182707
Innate Transcriptional Networks Activated in Bladder in Response to Uropathogenic Escherichia coli Drive Diverse Biological Pathways and Rapid Synthesis of IL-10 for Defense against Bacterial Urinary Tract Infection.	Early transcriptional activation events that occur in bladder immediately following bacterial urinary tract infection (UTI) are not well defined. In this study, we describe the whole bladder transcriptome of uropathogenic Escherichia coli (UPEC) cystitis in mice using genome-wide expression profiling to define the transcriptome of innate immune activation stemming from UPEC colonization of the bladder. Bladder RNA from female C57BL/6 mice, analyzed using 1.0 ST-Affymetrix microarrays, revealed e ...	2011	22184725
iberian wolf as a reservoir of extended-spectrum β-lactamase-producing escherichia coli of the tem, shv, and ctx-m groups.	the intensive use of antibiotics in human and veterinary medicine, associated with mechanisms of bacterial genetic transfer, caused a selective pressure that contributed to the dissemination of antimicrobial resistance in different bacteria groups and throughout different ecosystems. iberian wolf, due to his predatory and wild nature, may serve as an important indicator of environmental contamination with antimicrobial resistant bacteria. the aim of this study was to characterize the diversity o ...	2011	22185366
bactericidal activity of musca domestica cecropin (mdc) on multidrug-resistant clinical isolate of escherichia coli.	the housefly (musca domestica) larvae have been used clinically to cure osteomyelitis, decubital necrosis, lip boil, ecthyma and malnutritional stagnation ever since the ming/qing dynasty (1368 anno domini) till now, in china. in prior research, we have cloned and characterized a new gene of antimicrobial peptide cecropin from m. domestica larvae. this peptide was potently active against gram-positive and gram-negative bacteria standard strain. in the present study, we evaluated the possibility ...	2011	22202966
escherichia coli-induced epithelial hyporesponsiveness to secretagogues is associated with altered cftr localization.	both pathogenic and commensal strains of escherichia coli colonize the human intestinal tract. pathogenic strains differ only in the expression of virulence factors, many of which comprise a type iii secretion system (ttss). little is known regarding the effect of e. coli on the intestinal epithelial response to the secretagogues that drive ion secretion, despite its importance in causing clinically significant diarrhea. using ussing chambers to measure electrogenic ion transport of t84 intestin ...	2011	22212348
[preparation and identification of recombinant human neutrophil elastase].	to prepare a purified recombinant human neutrophil elastase (hne) using genetic engineering technology, and pave the way for the preparation of the antibody to hne and establishment of semen hne detection methods.	2011	22235674
incidence and virulence determinants of verocytotoxin-producing escherichia coli infections in brussels-capital region, belgium, 2008-2010.	the incidence of verocytotoxin-producing escherichia coli (vtec) was investigated by pcr in all human stools from universitair ziekenhuis brussel (uzb) and selected stools from six other hospital laboratories in brussels-capital region (belgium) collected between april 2008 and october 2010. selected stools to be included in this study were those from patients with hemolytic uremic syndrome (hus), patients with a history of bloody diarrhea, patients linked to clusters of diarrhea, children up to ...	2012	22238434
posttransplant lymphoproliferative disorder presenting as multiple cystic lesions in a renal transplant recipient.	we report a case of a 67-year-old man who experienced allograft dysfunction following a renal transplantation from a donation after cardiac death. the postoperative course was initially complicated by episodes of e. coli urinary sepsis causing pyrexia and a raised creatinine level. ultrasound scanning 5 weeks posttransplant revealed mild hydronephrosis with several parenchymal cystic areas measuring up to 2 cm with appearances suggestive of fungal balls. aspirated fluid again grew escherichia co ...	2012	22244123
safety evaluation of a natural eggshell membrane-derived product.	natural eggshell membrane (nem®) is a novel dietary ingredient that contains naturally occurring glycosaminoglycans and proteins essential for maintaining healthy joint and connective tissues. nem® was evaluated for safety via in vitro and in vivo toxicological studies. this included testing for cytotoxicity, genotoxicity, acute oral toxicity, and 90-day repeated-dose oral toxicity. nem® did not exhibit any cytotoxic effects at a dose of 100μg in an in vitro human cell viability assay after incu ...	2012	22245377
overexpression of potassium channel ether à go-go in human osteosarcoma.	human ether à go-go (heag) potassium channels are primarily expressed in brain but also frequently overexpressed in solid tumors, which could indicate their potential value for cancer diagnosis and therapy. heag1, one member of the heag subfamily, has been shown to play a role in neoplastic process. here we report the expression of heag1 in human osteosarcoma detected by a new polyclonal antibody. the full-length heag1 cdna was cloned from human osteosarcoma cell line mg63 by rt-pcr and expresse ...	2012	22248279