Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| role of branched-chain fatty acids in ph stress tolerance in listeria monocytogenes. | in alkaline conditions, listeria monocytogenes cells develop higher proportions of branched-chain fatty acids (fas), including more anteiso forms. in acid conditions, the opposite occurs. reduced growth of ph-sensitive mutants at adverse ph (5.0/9.0) was alleviated by the addition of 2-methylbutyrate (an anteiso-fa precursor), suggesting that anteiso-fas are important in adaptation to adverse ph. the balance between anteiso- and iso-fas may be more important than changes in the amounts and/or de ... | 2007 | 17114323 |
| cutting edge: il-12 inversely regulates t-bet and eomesodermin expression during pathogen-induced cd8+ t cell differentiation. | cytokines are critical determinants for specification of lineage-defining transcription factors of cd4+ t cell subsets. little is known, however, about how cytokines regulate expression of t-bet and eomesodermin (eomes) in effector and memory cd8+ t cells. we now report that il-12, a signature of cell-mediated immunity, represses eomes while positively regulating t-bet in effector cd8+ t cells during infection with listeria monocytogenes. after resolution of infection and abatement of il-12 sign ... | 2006 | 17114419 |
| ifn-beta-mediated up-regulation of cd1d in bacteria-infected apcs. | the expression of cd1d molecules is essential for the selection and activation of a unique subset of t cells, invariant nkt cells, which express limited tcr diversity and have been demonstrated to function in both regulatory and antimicrobial immune responses. although it has been reported that the levels of cd1d expression can be modulated during infection, the mechanisms that mediate this effect are poorly defined. in this study, we show that infection of dendritic cells and macrophages both i ... | 2006 | 17114455 |
| enteropathogenic escherichia coli, shigella flexneri, and listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals. | enteropathogenic escherichia coli, shigella flexneri, and listeria monocytogenes induce localized actin polymerization at the cytoplasmic face of the plasma membrane or within the host cytoplasm, creating unique actin-rich structures termed pedestals or actin tails. the process is known to be mediated by the actin-related protein 2 and 3 (arp2/3) complex, which in these cases acts downstream of neural wiskott-aldrich syndrome protein (n-wasp) or of a listerial functional homolog of wasp family p ... | 2007 | 17118974 |
| acta is required for crossing of the fetoplacental barrier by listeria monocytogenes. | the facultative intracellular bacterial pathogen listeria monocytogenes induces severe fetal infection during pregnancy. little is known about the molecular mechanisms allowing the maternofetal transmission of bacteria. in this work, we studied fetoplacental invasion by infecting mice with various mutants lacking virulence factors involved in the intracellular life cycle of l. monocytogenes. we found that the placenta was highly susceptible to bacteria, including avirulent bacteria, such as an l ... | 2007 | 17118980 |
| the role of the activated macrophage in clearing listeria monocytogenes infection. | macrophage activation often contributes to the strong immune response elicited upon infection. the ability of macrophages to become activated was discovered when sub-lethal primary infections of mice with the bacterium listeria monocytogenes provided protection against secondary infections through non-humoral immunity. l. monocytogenes infect and propagate in macrophages by escaping the phagosome into the cytosol, where they avoid humoral immune mediators. activated macrophages kill l. monocytog ... | 2007 | 17127272 |
| characterization of an hmg-coa reductase from listeria monocytogenes that exhibits dual coenzyme specificity. | hmg-coa reductase (hmgr) is an enzyme critical for cellular cholesterol synthesis in mammals and isoprenoid synthesis in certain eubacteria, catalyzing the nad(p)h-dependent reduction of hmg-coa to mevalonate. we have isolated the gene encoding hmg-coa reductase from listeria monocytogenes and expressed the recombinant 6x-his-tagged form in escherichia coli. using nad(p)(h), the enzyme catalyzes hmg-coa reduction approximately 200-fold more efficiently than mevalonate oxidation in vitro. the pur ... | 2006 | 17128979 |
| listeria monocytogenes meningitis and multiple brain abscesses in an immunocompetent host. favorable response to combination linezolid-meropenem treatment. | 2006 | 17129848 | |
| in the fvb/n her-2/neu transgenic mouse both peripheral and central tolerance limit the immune response targeting her-2/neu induced by listeria monocytogenes-based vaccines. | listeria monocytogenes-based vaccines for her-2/neu are capable of breaking tolerance in fvb/n rat her-2/neu transgenic mice. the growth of implanted nt-2 tumors, derived from a spontaneously occurring tumor in the fvb/n her-2/neu transgenic mouse, was significantly slower in these mice following vaccination with a series of l. monocytogenes-based vaccines for her-2/neu. mechanisms of t cell tolerance that exist in these transgenic mice include the absence of functional high avidity anti-her-2/n ... | 2007 | 17131121 |
| microflora of minimally processed frozen vegetables sold in gaborone, botswana. | two hundred samples of minimally processed, frozen, and prepacked potato chips, peas, corn, and a variety of combined vegetables from supermarkets in gaborone, botswana, were examined microbiologically. determination of aerobic mesophilic plate count, aerobic psychrotrophic plate count, lactic acid bacteria, yeasts and molds, coliforms, listeria spp., and staphylococcus aureus were done. chips had the lowest mean log values for all of the microorganisms enumerated except yeasts and molds. the me ... | 2006 | 17133799 |
| growth and stress resistance variation in culture broth among listeria monocytogenes strains of various serotypes and origins. | twenty-five listeria monocytogenes strains of various serotypes and sources, including clinical and food isolates associated with the same outbreaks, were characterized and compared based on growth rates and heat and acid death rates. growth was monitored in tryptic soy broth supplemented with 0.6% yeast extract (tsbye) at 4 and 30 degrees c for 32 days and 20 h, respectively. heat and acid stress responses in tsbye heated to 55 degrees c or acidified to ph 3.0 with lactic acid were evaluated fo ... | 2006 | 17133807 |
| quantifying nonthermal inactivation of listeria monocytogenes in european fermented sausages using bacteriocinogenic lactic acid bacteria or their bacteriocins: a case study for risk assessment. | listeria monocytogenes nctc10527 was examined with respect to its nonthermal inactivation kinetics in fermented sausages from four european countries: serbia-montenegro, hungary, croatia, and bosnia-herzegovina. the goal was to quantify the effect of fermentation and ripening conditions on l. monocytogenes with the simultaneous presence or absence of bacteriocin-producing lactic acid bacteria (i.e., lactobacillus sakei). different models were used to fit the experimental data and to calculate th ... | 2006 | 17133808 |
| intragastric inoculation with a cocktail of listeria monocytogenes strains does not potentiate the severity of infection in a/j mice compared to inoculation with the individual strains comprising the cocktail. | although multistrain cocktails of listeria monocytogenes are used in food inoculation experiments, no studies, to our knowledge, have been reported that use these cocktails in an intragastric mouse model. in this study, we used a five-strain l. monocytogenes cocktail consisting of strains scott a, mfs108, 101m, v7, and 310 and a four-strain l. monocytogenes cocktail containing strains scott a, egd, h7738, and f2365. here, we report that intragastric inoculation of anesthetized mice with approxim ... | 2006 | 17133809 |
| effects of microbial inhibitors and modified atmosphere packaging on growth of listeria monocytogenes and salmonella enterica typhimurium and on quality attributes of injected pork chops and sliced cured ham. | this study was designed to determine the inhibitory effects of modified atmosphere packaging (map), both alone and in combination with potassium lactate and sodium diacetate (plsd), on selected pathogens common to pork products. effects of the treatments on product quality also were assessed. the hypothesis was that high-co2 map would increase the effectiveness of plsd for inhibition of pork pathogens. fresh chops from untreated pork loins and loins that were injected with plsd were inoculated w ... | 2006 | 17133810 |
| assessment of environmental factors on listeria monocytogenes scott a inla gene expression by relative quantitative taqman real-time reverse transcriptase pcr. | several virulence factors are involved in listeria monocytogenes pathogenicity. l. monocytogenes internalins, particularly internalin a, are required for bacterial adhesion to and invasion of human intestinal epithelial cells. the expression of internalins is thus related to virulence. identification of conditions involved in regulating the expression of l. monocytogenes virulence factors is essential for developing targeted strategies to control listeriosis incidence and improving therapeutic a ... | 2006 | 17133822 |
| inactivation of the crp/fnr family of regulatory genes in listeria monocytogenes strain f2365 does not alter its heat resistance at 60 degrees c. | a surprising facet of the listeria monocytogenes genome is the presence of 15 genes that code for regulators in the crp/ fnr family and include the virulence regulator prfa. the genes under the transcriptional control of these regulators are currently undetermined, with the exception of some genes controlled by the major virulence regulator prfa. using 12 strains of l. monocytogenes, each with an inserted gene cassette that interrupts and renders nonfunctional a different l. monocytogenes strain ... | 2006 | 17133823 |
| stage of primary infection with lymphocytic choriomeningitis virus determines predisposition or resistance of mice to secondary bacterial infections. | we investigated the effect of a primary non-lethal infection with lymphocytic choriomeningitis virus (lcmv) on the course and outcome of a secondary infection with the gram-negative salmonella enterica serovar typhimurium or the gram-positive listeria monocytogenes in mice. we found that at each stage of the viral infection the susceptibility of mice to bacterial super-infections changes dramatically and depends also on whether the secondary infection is a gram-positive or gram-negative one. the ... | 2007 | 17136407 |
| a fret analysis to unravel the role of cholesterol in rac1 and pi 3-kinase activation in the inlb/met signalling pathway. | the signalling pathway for the hepatocyte growth factor receptor, met/hgf-r, is hijacked by the bacterial surface protein inlb to induce listeria monocytogenes entry into non-phagocytic cells. we previously showed that listeria invades host cells by interacting with specialized microdomains of the host plasma membrane called lipid rafts. in this study, we analysed in living cells signalling events that are crucial for listeria entry using a fluorescence resonance energy transfer-based microscopi ... | 2007 | 17140407 |
| modeling the growth of listeria monocytogenes based on a time to detect model in culture media and frankfurters. | in the current study, temperature dependent growth of listeria monocytogenes strain h7776, with an initial inoculum level of approximately 0.1 cfu/ml or g, was modeled based on "time to detect" (ttd) using the arrhenius equation. the activation energies (e(a)) obtained from specific growth rate and lag phase duration in tryptic soy broth (tsb) and frankfurters were compared. at 4 degrees c, the ttd for l. monocytogenes was 217 h in tsb and 48 h in portions of frankfurters. the ttd decreased as t ... | 2007 | 17140686 |
| management of microbiological safety of ready-to-eat meat products by mathematical modelling: listeria monocytogenes as an example. | the recent commission regulation (ec) no 2073/2005 establishes microbiological criteria in foods. for the pathogen listeria monocytogenes in the category ready-to-eat foods able to support its growth, other than those intended for infants and for special medical purposes, two different microbiological criteria are proposed: (i) l. monocytogenes levels should be <100 cfu/g throughout the shelf-life of the product, (ii) absence in 25 g of the product at the stage before the food has left the immed ... | 2007 | 17140689 |
| a case of listeria meningitis associated with increased adenosine deaminase in cerebrospinal fluid. | we present, to the best of our knowledge, the first case of listeria meningitis with increased adenosine deaminase (ada) in cerebrospinal fluid. although some cases of other etiologies were also reported, this report informs those who manage patients with meningitis that not only mycobacterium and brucella spp. but also other facultative agents can increase ada levels in cerebrospinal fluid, possibly through monocyte-macrophage activation. | 2007 | 17141462 |
| differentiation of listeria monocytogenes serovars by using artificial neural network analysis of fourier-transformed infrared spectra. | a classification system based on fourier transform infrared (ftir) spectroscopy combined with artificial neural network analysis was designed to differentiate 12 serovars of listeria monocytogenes using a reference database of 106 well-defined strains. external validation was performed using a test set of another 166 l. monocytogenes strains. the o antigens (serogroup) of 164 strains (98.8%) could be identified correctly, and h antigens were correctly determined in 152 (91.6%) of the test strain ... | 2007 | 17142376 |
| persistent antigen presentation after acute vesicular stomatitis virus infection. | long-term antigen expression is believed to play an important role in modulation of t-cell responses to chronic virus infections. however, recent studies suggest that immune responses may occur late after apparently acute infections. we have now analyzed the cd8 t-cell response to vesicular stomatitis virus (vsv), which is thought to cause to an infection characterized by rapid virus clearance by innate and adaptive immune system components. unexpectedly, virus-encoded antigen was detectable mor ... | 2007 | 17151119 |
| interval mapping of quantitative trait loci for time-to-event data with the proportional hazards mixture cure model. | interval mapping using normal mixture models has been an important tool for analyzing quantitative traits in experimental organisms. when the primary phenotype is time-to-event, it is natural to use survival models such as cox's proportional hazards model instead of normal mixtures to model the phenotype distribution. an extra challenge for modeling time-to-event data is that the underlying population may consist of susceptible and nonsusceptible subjects. in this article, we propose a semiparam ... | 2006 | 17156279 |
| a bifunctional o-glcnac transferase governs flagellar motility through anti-repression. | flagellar motility is an essential mechanism by which bacteria adapt to and survive in diverse environments. although flagella confer an advantage to many bacterial pathogens for colonization during infection, bacterial flagellins also stimulate host innate immune responses. consequently, many bacterial pathogens down-regulate flagella production following initial infection. listeria monocytogenes is a facultative intracellular pathogen that represses transcription of flagellar motility genes at ... | 2006 | 17158746 |
| improved antibacterial host defense and altered peripheral granulocyte homeostasis in mice lacking the adhesion class g protein receptor cd97. | cd97 is a member of the adhesion family of g protein-coupled receptors. alternatively spliced forms of cd97 bind integrins alpha5beta1 and alphavbeta3, decay accelerating factor, or dermatan sulfate. cd97 is expressed on myeloid cells at high levels and a variety of other cell types at lower levels. little is known about the physiological function of cd97. to begin dissecting the function of cd97, we evaluated the immune response of cd97 null mice to systemic infection by listeria monocytogenes. ... | 2007 | 17158902 |
| characterization of early gamma interferon (ifn-gamma) expression during murine listeriosis: identification of nk1.1+ cd11c+ cells as the primary ifn-gamma-expressing cells. | though it is well established that gamma interferon (ifn-gamma) is crucial to the early innate defense of murine listeriosis, its sources remain controversial. in this study, intracellular cytokine staining of ifn-gamma-expressing splenocytes early after listeria monocytogenes infection revealed that nk1.1(+), cd11c(+), cd8(+) t, and cd4(+) t cells expressed ifn-gamma 24 h after infection. contrary to the previous report, most ifn-gamma(+) dendritic cells (dc) were cd8alpha(-) dc. unexpectedly, ... | 2007 | 17158904 |
| listeria monocytogenes in a young patient with non hodgkins lymphoma: case report. | listeria monocytogenes is an intracellular food-borne pathogen, widely distributed in the environment, which rarely causes clinical infection in healthy people, but may cause severe disease in immunocompromised patients. a case of listeriosis is certified in an immunocompromised patient, thus confirming this microorganism to be an opportunistic human pathogen. | 2006 | 17166414 |
| [spontaneous bacterial peritonitis caused by listeria in a patient with cirrhosis: case report]. | infections caused by listeria monocytogenes are usually found in cattle and occasionally appear in humans, particularly pregnant women and immunocompromised individuals. peritonitis by listeria monocytogenes is a rare but dangerous condition that must be recognized early, since it requires a specific treatment. we report a 31 year-old male with alcoholic cirrhosis that developed ascites with abdominal pain and fever. the peritoneal fluid culture yielded listeria monocytogenes. the patients was i ... | 2006 | 17171220 |
| identification of ispc, an 86-kilodalton protein target of humoral immune response to infection with listeria monocytogenes serotype 4b, as a novel surface autolysin. | we identified and biochemically characterized a novel surface-localized autolysin from listeria monocytogenes serotype 4b, an 86-kda protein consisting of 774 amino acids and known from our previous studies as the target (designated ispc) of the humoral immune response to listerial infection. recombinant ispc, expressed in escherichia coli, was purified and used to raise specific rabbit polyclonal antibodies for protein characterization. the native ispc was detected in all growth phases at a rel ... | 2007 | 17172332 |
| sensitive listeria spp. immunoassay based on europium(iii) nanoparticulate labels using time-resolved fluorescence. | listeria spp. are gram-positive rod shaped bacteria found universally in the environment. pathogenic listeria monocytogenes is seldom harmful to healthy adults, but can cause serious disease, listeriosis, especially to pregnant women, neonates, and elderly or immunocompromised people. conventional methods for screening listeria in food samples are time consuming and laborious, involving the use of a range of liquid media and plate cultures. in the current study, the total analysis time was short ... | 2007 | 17173997 |
| the effects of growth temperature and growth phase on the inactivation of listeria monocytogenes in whole milk subject to high pressure processing. | the aim of this study was to explore the effect of a wide range of growth temperatures, growth phases and plating media on the inactivation of listeria monocytogenes by high pressure processing (hpp). in part one, l. monocytogenes was grown to mid-stationary phase at 4, 15, 25, 35 or 43 degrees c, inoculated into whole uht milk at approximately 10(7) cfu/ml and high pressure processed at 400 mpa at room temperature (20-25 degrees c). afterward, the hpp milk was plated on tryptic soy yeast extrac ... | 2007 | 17173999 |
| development and application of multiple-locus variable number of tandem repeat analysis (mlva) to subtype a collection of listeria monocytogenes. | in this study we report the development and application of a multiple-locus variable number of tandem repeat analysis (mlva) strategy for subtyping listeria monocytogenes. genome profiles of a collection of forty-five food-borne l. monocytogenes isolates were compared using mlva. these isolates were obtained as part of an active surveillance programme of foods in the south-east region of ireland. mlva successfully discriminated amongst the isolates. the method was easy to perform, relatively fas ... | 2007 | 17174430 |
| factors associated with listeria monocytogenes contamination of cold-smoked pork products produced in latvia and lithuania. | a total of 312 samples of sliced, vacuum packaged, cold-smoked pork from 15 meat processing plants in latvia and lithuania, obtained over a 15-month period from 2003 until 2004, were analyzed for the presence of listeria monocytogenes at the end of their shelf-life. overall, 120 samples (38%) tested positive for l. monocytogenes. despite the long storing period, the levels of l. monocytogenes in cold-smoked pork products were low. manufacturing processes were studied at seven meat processing pla ... | 2007 | 17174432 |
| [molecular typing of listeria monocytogenes isolated from clinical and food samples]. | listeria monocytogenes is an emergent foodborne pathogen acquired by the ingestion of contaminated food. this bacterium causes a disease called listeriosis, whose mortality rate world wide is around 20% to 30%, reaching up to 80% in cases of neonatal infections. the random amplified polymorphic dna technique allows different isolates to be distinguished and characterized at the molecular level, which can provide useful information about the diversity of this pathogen in colombia. | 2006 | 17176008 |
| six-color segmentation of multicolor images in the infection studies of listeria monocytogenes. | multiple immunofluorescent staining is a powerful strategy for visualizing the spatial and temporal relationship between antigens, cell populations, and tissue components in histological sections. to segment different cell populations from the multicolor image generated by immunostaining based on color addition theory, a systems approach is proposed for automatic segmentation of six colors. after image acquisition and processing, images are automatically segmented with the proposed approach and ... | 2007 | 17177276 |
| hyponatraemia in adults with community-acquired bacterial meningitis. | hyponatraemia in adults with bacterial meningitis has been described as a common complication, but its true prevalence and clinical importance are unknown. | 2007 | 17178734 |
| microbial composition in bioaerosols of a high-throughput chicken-slaughtering facility. | the microbial composition of the air in various areas of a high-throughput chicken-slaughtering facility was investigated. over a 4-mo period, 6 processing areas were sampled, and the influence of environmental factors was monitored. the highest counts of microorganisms were recorded in the initial stages of processing, comprising the receiving-killing and defeathering areas, whereas counts decreased toward the evisceration, air-chilling, packaging, and dispatch areas. maximum microbial counts w ... | 2007 | 17179429 |
| marination of turkey breast fillets to control the growth of listeria monocytogenes and improve meat quality in deli loaves. | turkey deli loaves were evaluated using organic marinades in the raw product to control the growth of listeria monocytogenes (lm) and improve meat quality in the cooked product. treatments included sodium tripolyphosphate (stp; 0.45%, control), sodium lactate (3%), sodium diacetate (0.25%), sodium citrate (0.75%), and sodium lactate (3%)/sodium diacetate (0.25%) combination, all containing 1.5% salt. data collected in the 2 trials included ph; lightness, redness, and yellowness; bind ability; co ... | 2007 | 17179430 |
| genotypic characterization of listeria spp. isolated from fresh water fish. | a total of 200 samples (muscles and viscera, 100 of each) of fresh water fish, walking catfish (clarias batrachus) were screened for listeria spp. all the samples were subjected to a two-step enrichment followed by plating on selective media. confirmation of the isolates was on the basis of biochemical characters, haemolysis on blood agar and christie, atkins, munch petersen test. a total of 39 isolates of listeria spp. were recovered. of these 26 (67%), 8 (21%), 3 (8%) and 2 (5%) were listeria ... | 2007 | 17182144 |
| activation of type b t cells after protein immunization reveals novel pathways of in vivo presentation of peptides. | type b t cells recognize a peptide-mhc conformer generated in recycling endosomes and eliminated by h2-dm in late endosomes; as a result, they recognize exogenous peptide, but fail to respond to the identical epitope generated from the native protein. to investigate the behavior of these cells in vivo, we generated mice transgenic for a type b tcr recognizing the 48-62 epitope of hen egg white lysozyme (hel) presented by i-a(k). type b t cells responded only to peptide ex vivo, but responded in ... | 2007 | 17182547 |
| the microbiological quality of hot water-washed broccoli florets and cut green beans. | to determine the effect of hot water washing on the microbiological quality of cut broccoli florets and trimmed green beans. | 2007 | 17184318 |
| partial characterization of bacteriocins produced by human lactococcus lactis and pediococccus acidilactici isolates. | the aim of this study was to isolate bacteriocin-producing lactic acid bacteria (lab) from human intestine. | 2007 | 17184344 |
| influence of ph, water activity and acetic acid concentration on listeria monocytogenes at 7 degrees c: data collection for the development of a growth/no growth model. | growth/no growth models can be used to determine the chance that microorganisms will grow in specific environmental conditions. as a consequence, these models are of interest in the assessment of the safety of foods which can be contaminated with food pathogens. in this paper, growth/no growth data for listeria monocytogenes (in a monoculture and in a mixed strain culture) are presented. the data were gathered at 7 degrees c in nutrient broth with different combinations of environmental factors ... | 2007 | 17184866 |
| the mutations lys 114 --> gln and asp 126 --> asn disrupt an intersubunit salt bridge and convert listeria innocua dps into its natural mutant listeria monocytogenes dps. effects on protein stability at low ph. | the stability of the dodecameric listeria monocytogenes dps has been compared with that of the listeria innocua protein. the two proteins differ only in two amino acid residues that form an intersubunit salt-bridge in l. innocua dps. this salt-bridge is replaced by a hydrogen bonding network in l. monocytogenes dps as revealed by the x-ray crystal structure. the resistance to low ph and high temperature was assayed for both dps proteins under equilibrium conditions and kinetically. despite the i ... | 2007 | 17186524 |
| microbiological baseline study of broiler chickens at swedish slaughterhouses. | this 1-year study was conducted to estimate the prevalence and concentrations of pathogenic and indicator bacteria on swedish broiler chickens. a total of 636 chilled carcasses were collected from 10 slaughterhouses and sent to the national food administration for analyses of carcass rinses. no carcasses were positive for salmonella. campylobacter, predominantly campylobacter jejuni, were detected on 15% (by enrichment) or 14% (by direct plating) of the carcasses. with one exception, all samples ... | 2006 | 17186653 |
| international life sciences institute north america listeria monocytogenes strain collection: development of standard listeria monocytogenes strain sets for research and validation studies. | research and development efforts on bacterial foodborne pathogens, including the development of novel detection and subtyping methods, as well as validation studies for intervention strategies can greatly be enhanced through the availability and use of standardized strain collections. these types of strain collections are available for some foodborne pathogens, such as salmonella and escherichia coli. we have developed a standard listeria monocytogenes strain collection that has not been previou ... | 2006 | 17186661 |
| transfer of listeria monocytogenes during slicing of turkey breast, bologna, and salami with simulated kitchen knives. | in response to continued concerns regarding listeria cross-contamination during the slicing of deli meats, a series of specially prepared grade 304 and 316 stainless steel kitchen knife blades was inoculated with a six-strain listeria monocytogenes cocktail (10(8), 10(5), and 10(3) cfu per blade) composed of two weak, two medium, and two strong biofilm-forming strains. the blades were then attached to an instron 5565 electromechanical compression analyzer and used to slice whole chubs of delicat ... | 2006 | 17186662 |
| inhibition and inactivation of listeria monocytogenes and escherichia coli o157:h7 colony biofilms by micellar-encapsulated eugenol and carvacrol. | the antimicrobial efficacy of carvacrol and eugenol, two essential oil compounds, encapsulated in a micellar nonionic surfactant solution on four strains of listeria monocytogenes (scott a, 101, 108, and 310) and four strains of escherichia coli o157:h7 (h1730, e0019, f4546, and 932) growing as colony biofilms was investigated. carvacrol and eugenol were encapsulated in surfynol 485w at concentrations ranging from 0.3 to 0.9% (wt/wt) at a surfactant concentration of 5% (wt/wt). colony biofilms w ... | 2006 | 17186663 |
| irradiation inactivation of listeria monocytogenes in low-fat ground pork at freezing and refrigeration temperatures. | gamma radiation effectively controls listeria monocytogenes in uncooked and in ready-to-eat foods. this study was conducted to determine if gamma radiation could be used to control l. monocytogenes in ground pork. ground pork was contaminated with l. monocytogenes, kept at refrigeration (4 degrees c), chilling (0 degrees c), and freezing (-18 degrees c) temperatures overnight, exposed to gamma radiation and stored at 4 degrees c for 7 days, and at 0 and -18 degrees c for 60 days. following irrad ... | 2006 | 17186664 |
| effect of gamma radiation and oregano essential oil on murein and atp concentration of listeria monocytogenes. | the effects of gamma radiation and of oregano essential oil alone or in combination with radiation on murein composition of listeria monocytogenes and on the intracellular and extracellular concentration of atp were evaluated. the bacterial strain was treated with two radiation doses, 1.2 kgy to induce cell damage and 3.5 kgy to cause cell death. oregano essential oil was used at 0.020 and 0.025% (wt/vol), which is the mic. all treatments had a significant effect (p < or = 0.05) on the murein co ... | 2006 | 17186665 |
| heat inactivation of listeria monocytogenes and salmonella enterica serovar typhi in a typical bologna matrix during an industrial cooking-cooling cycle. | the heat resistance of salmonella enterica serovar typhi pf-724 and listeria monocytogenes 2812 was determined in a commercial bologna batter. the heat inactivation of the two bacterial species was also studied in a semiautomatic pilot smokehouse under cooking conditions that reproduced an industrial bologna process. s. enterica serovar typhi pf-724 was less heat resistant than l. monocytogenes 2812. the d-values (times required to reduce the population by 1 logarithmic cycle) for s. enterica se ... | 2006 | 17186675 |
| role of uvra in the growth and survival of listeria monocytogenes under uv radiation and acid and bile stress. | listeria monocytogenes encounters numerous stresses both in the food environment and during infection of the host. the ability to survive and tolerate bile and low ph conditions, which are two major stresses, is of particular importance for survival within the host. the uvra gene in other bacteria is involved in the repair of acid-induced dna damage and in adaptation to low ph. thus, a uvra in-frame deletion mutant was constructed to identify the role of uvra in the growth and survival of l. mon ... | 2006 | 17186676 |
| survival and growth of foodborne pathogens during cooking and storage of oriental-style rice cakes. | fresh cooked rice cakes for retail sale are typically held at room temperature because refrigeration dramatically reduces their quality. room temperature, high water activity, and a ph of > 4.6 provided an environment conducive to pathogen growth. to date, no studies have been published regarding survival and growth of foodborne pathogens in fresh cooked rice cakes. this study was undertaken to investigate the effect of steam cooking on foodborne pathogens and their subsequent growth in five var ... | 2006 | 17186677 |
| the adaptor protein card9 is required for innate immune responses to intracellular pathogens. | the caspase-recruitment domain-containing adaptor protein card9 regulates the innate signaling responses to fungal infection. here we show that card9 is required for innate immune responses against intracellular pathogens. we generated card9(-/-) mice and found that card9-deficient macrophages had defects in activation of the kinases p38 and jnk but not of transcription factor nf-kappab after bacterial and viral infection. card9-deficient mice failed to clear infection and showed altered cytokin ... | 2007 | 17187069 |
| use of linear, weibull, and log-logistic functions to model pressure inactivation of seven foodborne pathogens in milk. | survival curves of six foodborne pathogens suspended in ultra high-temperature (uht) whole milk and exposed to high hydrostatic pressure at 21.5 degrees c were obtained. vibrio parahaemolyticus was treated at 300 mpa and other pathogens, listeria monocytogenes, escherichia coli o157:h7, salmonella enterica serovar enteritidis, salmonella enterica serovar typhimurium, and staphylococcus aureus were treated at 600 mpa. all the survival curves showed a rapid initial drop in bacterial counts followe ... | 2007 | 17188197 |
| growth of listeria monocytogenes in egg salad and pasta salad formulated with mayonnaise of various ph and stored at refrigerated and abuse temperatures. | this study investigated and modeled the behavior of listeria monocytogenes in egg salad and pasta salad as affected by mayonnaise ph (3.8, 4.2, 4.6, and 5.0) and storage temperature (4, 8, and 12 degrees c). at each storage temperature, l. monocytogenes was able to grow in both salads regardless of the mayonnaise ph. the lag-phase durations (lpd) of l. monocytogenes in egg salad ranged from 33 to 85, 15 to 50, and 0 to 19 h, and the growth rates (gr) ranged from 0.0187 to 0.0318, 0.0387 to 0.051 ... | 2007 | 17188199 |
| phenotypic and technological diversity of lactic acid bacteria and staphylococci isolated from traditionally fermented sausages in southern greece. | the physicochemical and microbiological characteristics of spontaneously fermented sausages made by two medium-sized enterprises (mse) located in southern greece have been studied. a total of 300 lactic acid bacteria and 300 staphylococcal strains have been isolated and identified by their physiological characteristics. lactobacillus plantarum strains were found to dominate the lactic acid bacteria microbiota in most of the cases with l. sakei strains prevailing in some of them and l. rhamnosus ... | 2007 | 17188204 |
| effect of industrial and natural biocides on spoilage, pathogenic and technological strains grown in biofilm. | this study aimed at investigating bactericide solutions effective on spoilage and pathogenic bacteria while preserving technological bacteria. two compounds of essential oil (thymol and eugenol), one essential oil of satureja thymbra and two industrial biocides (pe 270-30, brillo) were tested on technological strains (staphylococcus equorum, staphylococcus succinus and lactobacillus sakei) grown in monoculture biofilm and on a mixed biofilm of pathogenic bacteria (staphylococcus aureus, listeria ... | 2007 | 17188206 |
| antimicrobial and cytotoxic knotwood extracts and related pure compounds and their effects on food-associated microorganisms. | knotwood or bark extracts prepared from 30 species of hard and soft wood trees as well as selected pure compounds (lignans, stilbenes and flavonoids) were assayed for their antimicrobial activity against a battery of both gram positive and negative bacteria, yeasts, and filamentous fungi (bacillus cereus, staphylococcus aureus, listeria monocytogenes, lactobacillus plantarum, escherichia coli, salmonella infantis, pseudomonas fluorescens, candida albicans, saccharomyces cerevisiae, aspergillus f ... | 2007 | 17188387 |
| genetic diversity of listeria monocytogenes recovered from infected persons and pork, seafood and dairy products on retail sale in france during 2000 and 2001. | growth of the food-borne human pathogen listeria monocytogenes to large numbers in ready-to-eat food products greatly increases the risk of disease for susceptible consumers. a better knowledge of the population structure of l. monocytogenes present in retailed food could allow better prevention strategies to be developed. we present the analysis of 450 l. monocytogenes isolates, 179 responsible for sporadic human cases of listeriosis and 271 isolated from foods collected from retailers. all iso ... | 2007 | 17188773 |
| effect of preinoculation growth media and fat levels on thermal inactivation of a serotype 4b strain of listeria monocytogenes in frankfurter slurries. | preinoculation growth conditions and fat levels were evaluated for effects on the heat resistance of listeria monocytogenes strain mfs 102 in formulated frankfurter slurries and on frankfurter surfaces. comparison of linear inactivation rates (d-values) for cells heated in frankfurter slurry showed that growth conditions were significant (p<0.05) factors affecting subsequent thermal resistance. the average d(60 degrees c)-values for the five preinoculation growth media tested from most resistant ... | 2007 | 17189761 |
| modelling thermal inactivation of listeria monocytogenes in sucrose solutions of various water activities. | the heat resistance of listeria monocytogenes was determined in sucrose solutions with water activity (a(w)) ranging from 0.99 to 0.90. at all temperatures investigated shape of the survival curves depended on the a(w) of the treatment medium. the survival curves for a(w)=0.99 appeared to be linear, for a(w)=0.96 were slightly upwardly concaved and for a(w)=0.93 and 0.90 were markedly concave upward. a mathematical model based on the weibull distribution provided a good fit for all the survival ... | 2007 | 17189763 |
| assessing biofilm formation by listeria monocytogenes strains. | when a microtitre plate assay was used to quantify biofilm production by listeria monocytogenes strains following growth in tryptone soy broth (tsb) for 48 h at 20 degrees c, 127 of 138 strains (92.0%) were classified as weak, 9 of 138 strains (6.5%) as moderate and only 2 of 138 strains (1.5%) as strong biofilm formers. the strains included environmental, animal, food (persistent and sporadic strains) and clinical isolates previously typed using esterase electrophoresis (ese) and multi-locus en ... | 2007 | 17189764 |
| effect of co2 on the resuscitation of listeria monocytogenes injured by mild inactivation techniques. | 2006 | 17191465 | |
| microbial safety of mayonnaise based salads: a growth/no growth model for listeria monocytogenes as a predictive tool. | 2006 | 17191531 | |
| water activity of bacterial suspension media unable to account for the baroprotective effect of solute concentration on the inactivation of listeria monocytogenes by high hydrostatic pressure. | inactivation of listeria monocytogenes (10(8) cfu/ml) by high hydrostatic pressure (hhp) from 400 to 600 mpa at 25 degrees c for 10 min was investigated with various concentrations of sodium chloride, sucrose, and sodium phosphate buffer solutions. sodium chloride significantly inhibited hhp-induced inactivation of l. monocytogenes at concentrations higher than 2.6 m. a low concentration of sodium chloride within 1.7 m had no effect on hhp-induced inactivation. almost complete inactivation at re ... | 2007 | 17196694 |
| listeria monocytogenes in multiple habitats and host populations: review of available data for mathematical modeling. | listeria monocytogenes has the ability to survive and multiply in diverse habitats and to cause infection in a variety of animal species and humans. we evaluated the literature on survival and multiplication within and transmission among multiple host populations and habitats, including man, sewage, general environment (soil, water, and vegetation), silage (fermented plant material), animals (including wild and domestic animals), and food processing plants. the available knowledge on l. monocyto ... | 2006 | 17199514 |
| evaluation of effects of primary and secondary enrichment for the detection of listeria monocytogenes by real-time pcr in retail ground chicken meat. | a sybr green i based real-time pcr assay with inla-specific oligonucleotide primers was developed for easy and rapid detection of listeria monocytogenes in a model food that usually has a high incidence of contamination with this pathogen. results with pure cultures and artificially contaminated chicken meat samples indicate that the pcr assay was highly specific and sensitive. the melting point analysis of the 160 bp amplified dna fragment was different for l. monocytogenes isolates of the two ... | 2006 | 17199516 |
| pulsed-field gel electrophoresis (pfge) analysis of temporally matched listeria monocytogenes isolates from human clinical cases, foods, ruminant farms, and urban and natural environments reveals source-associated as well as widely distributed pfge types. | a total of 495 temporally and geographically matched listeria monocytogenes isolates from human clinical cases, foods, ruminant farms, and urban and natural environments were used to investigate l. monocytogenes pulsed-field gel electrophoresis (pfge) type diversity. two-enzyme (asci and apai) pfge discriminated 310 pfge types and exhibited higher overall discriminatory power (simpson's index of discrimination [d] = 0.995) than either ecori ribotyping (d = 0.950) or asci or apai single-enzyme pf ... | 2007 | 17202278 |
| autophagy limits listeria monocytogenes intracellular growth in the early phase of primary infection. | autophagy has been recently proposed to be a component of the innate cellular immune response against several types of intracellular microorganisms. however, other intracellular bacteria including listeria monocytogenes have been thought to evade the autophagic cellular surveillance. here, we show that cellular infection by l. monocytogenes induces an autophagic response, which inhibits the growth of both the wild-type and a deltaacta mutant strain, impaired in cell-to-cell spreading. the onset ... | 2007 | 17204850 |
| antimicrobial resistance of listeria spp. recovered from processed bison. | the current study examined the antimicrobial susceptibility of 86 listeria spp. isolated from processed bison carcasses. materials and methods: susceptibility to 25 antimicrobial agents was determined using e-test and national antimicrobial resistance monitoring system (narms) panels. most listeria isolates (88-98%) exhibited resistance to bacitracin, oxacillin, cefotaxime, and fosfomycin. resistance to tetracycline (18.6%) was also common. of the 16 tetracycline-resistant listeria isolates, 15 ... | 2007 | 17209820 |
| endocytic delivery of vancomycin mediated by a synthetic cell surface receptor: rescue of bacterially infected mammalian cells and tissue targeting in vivo. | 2007 | 17212394 | |
| the spectrum of antimicrobial activity of the bacteriocin subtilosin a. | bacterocins are antimicrobial peptides produced by bacteria with a relatively narrow range of activity against closely related organisms. subtilosin a is a bacteriocin produced by bacillus subtilis that has activity against listeria monocytogenes, which might indicate antimicrobial activity unusual for bacteriocins. | 2007 | 17213266 |
| listeria monocytogenes mutants carrying newcastle disease virus f gene fused to its acta and plcb: in vitro expression and immunogenicity in chickens. | recombinant listeria monocytogenes mutants carrying newcastle disease virus (ndv) fusion protein gene f were constructed by homologous recombination. ndv f or its truncated fragment fa was used as the model heterologous gene to be integrated into acta or plcb downstream of their signal sequences. correct orientation of the inserted genes was verified by polymerase chain reaction amplification of f or fa. the inserted f and fa were expressed in the two recombinants lm-deltaacta-f and lm-deltaplcb ... | 2007 | 17213959 |
| multi-virulence-locus sequence typing identifies single nucleotide polymorphisms which differentiate epidemic clones and outbreak strains of listeria monocytogenes. | a recently developed multi-virulence-locus sequence typing (mvlst) method showed improved discriminatory power for subtyping genetically diverse listeria monocytogenes isolates and identified epidemic clone ii isolates associated with two recent u.s. multistate listeriosis outbreaks. to evaluate the ability of mvlst to distinguish other epidemic clones and outbreak strains of l. monocytogenes, 58 outbreak-related isolates from 14 outbreaks and 49 unrelated isolates were analyzed. results showed ... | 2007 | 17215339 |
| a critical role for peptidoglycan n-deacetylation in listeria evasion from the host innate immune system. | listeria monocytogenes is a human intracellular pathogen that is able to survive in the gastrointestinal environment and replicate in macrophages, thus bypassing the early innate immune defenses. peptidoglycan (pg) is an essential component of the bacterial cell wall readily exposed to the host and, thus, an important target for the innate immune system. characterization of the pg from l. monocytogenes demonstrated deacetylation of n-acetylglucosamine residues. we identified a pg n-deacetylase g ... | 2007 | 17215377 |
| use of microbial antagonism to reduce pathogen levels on produce and meat products: a review. | lactic acid bacteria (lab) are often utilized to control food-borne pathogens on produce and on cooked, fermented, or refrigerated meats. most research to date has focused on the inhibition of listeria monocytogenes, escherichia coli o157:h7, salmonella, clostridium botulinum, and spoilage microorganisms. lab are excellent candidates for reducing pathogen levels on foods because they inhibit the growth of these microorganisms through various mechanisms without causing unacceptable sensory change ... | 2006 | 17215892 |
| bacteriocin activity of enterococci from rabbits. | the antimicrobial spectrum of bacteriocin-producing enterococcal isolates from rabbits faeces, the biochemical characterization of bacteriocins, and their molecular mass and the presence of structural genes for bacteriocin production were investigated. among enterococci selected from rabbit faeces, six strains of enterococcus faecium (ef2019, ef1819, ef2119, ef1839, ef529, ef24/10) showed inhibitory activity against the indicators e. avium ea5, listeria innocua lmg13568 and listeria monocytogene ... | 2007 | 17216320 |
| [a fatal case of listeria monocytogenes sepsis and meningitis in a patient with wegener's granulomatosis]. | the bacillus listeria monocytogenes is widely distributed in the environment. listeria monocytogenes most often causes infection in the neonates, pregnant women, elderly and immunosuppressed persons. we report on a case of fatal sepsis and meningitis in a 59-year-old woman receiving cyclophosphamide and glucocorticoid therapy for wegener's granulomatosis over a 10-year period. listeriosis should be suspected in case of sepsis and/or meningitis in patients who receive immunosuppressive agents. si ... | 2006 | 17217109 |
| a new device for rapid evaluation of biofilm formation potential by bacteria. | this work describes the implementation of a new assay named the biofilm ring test to evaluate the ability of bacteria to form biofilms. this assay is based on the immobilisation (or not) of magnetic beads embedded by bacterial aggregates or mats (patented concept). it is realised on modified polystyrene 96-wells microtiter plates with individual 8-wells slides. the kinetic of biofilm formation of four bacterial species, listeria monocytogenes, escherichia coli, staphylococcus carnosus and staphy ... | 2007 | 17218029 |
| the presence of the internalin gene in natural atypically hemolytic listeria innocua strains suggests descent from l. monocytogenes. | the atypical hemolytic listeria innocua strains prl/nw 15b95 and j1-023 were previously shown to contain gene clusters analogous to the pathogenicity island (lipi-1) present in the related foodborne gram-positive facultative intracellular pathogen listeria monocytogenes, which causes listeriosis. lipi-1 includes the hemolysin gene, thus explaining the hemolytic activity of the atypical l. innocua strains. no other l. monocytogenes-specific virulence genes were found to be present. in order to in ... | 2007 | 17220266 |
| daptomycin susceptibility of unusual gram-positive bacteria: comparison of results obtained by the etest and the broth microdilution method. | mics of daptomycin, linezolid, and vancomycin against 212 isolates, including listeria monocytogenes and pediococcus, leuconostoc, rhodococcus, and nocardia spp., were determined by the broth microdilution method; daptomycin mics were also determined by the etest. except with those for leuconostoc spp., daptomycin etest mics showed >90% agreement with mics obtained by the broth microdilution method. | 2007 | 17220420 |
| differential function of listeria monocytogenes listeriolysin o and phospholipases c in vacuolar dissolution following cell-to-cell spread. | we investigated the role of listeriolysin o (llo) and the bacterial phospholipases pi-plc and pc-plc in cell-to-cell spread of listeria monocytogenes. we showed that llo is essential for cell-to-cell spread in primary murine macrophages. electron micrographs revealed that in the absence of continued llo expression, bacteria remain trapped in secondary spreading vacuoles having either a double or single membrane. in bacteria lacking pi-plc and pc-plc, cessation of llo expression after initiation ... | 2007 | 17222191 |
| immunohistochemical study of the reticular and vascular network of mouse lymph node using vibratome sections. | the function of lymph nodes is greatly influenced by their unique microanatomy, in which distinct subpopulations of cells are compartmentalized by a meshwork of reticular cells and fibres, specialized blood and lymphatic vessels and nerves. using antibodies against extracellular matrix (ecm) proteins (fibronectin, collagen iv and laminin), proteoglycan (perlecan), and a fibroblastic marker (ertr-7), the distribution and molecular organization of the system of reticular fibres was investigated by ... | 2007 | 17224179 |
| quantitative assessment of hard surface disinfectant activity against the foodborne pathogen listeria monocytogenes. | listeria monocytogenes is an important foodborne pathogen that must be controlled to ensure food safety. for the years 2003 and 2004, l. monocytogenes caused 20 deaths per 100 listeriosis cases and was responsible for most food recalls for pathogen contamination. the objective of this work was to develop a quantitative method to assess disinfectant activity against l. monocytogenes. standard procedures for testing disinfectants against 3 bacteria are described in the aoac official methods of ana ... | 2006 | 17225610 |
| effect of heat and high-pressure treatments on microbiological quality and immunoglobulin g stability of caprine colostrum. | caprine colostrums (6 batches) were subjected to heat (56 degrees c for 60 min and 63 degrees c for 30 min) and high-pressure (400 and 500 mpa for 10 min at 20 degrees c) treatments at laboratory scale, and analyses of the main microbial groups and the extent of igg denaturation (determined by immunodiffusion) were performed. overall mean microbial values in raw colostrums were: total count, 5.55 log cfu/ml; enterobacteriaceae, 2.64 log cfu/ml; lactococci, 5.41 log cfu/ml; lactobacilli, 2.34 log ... | 2007 | 17235160 |
| expression and function of synaptotagmin vii in ctls. | the ca(2+) sensor synaptotagmin (syt) vii regulates the exocytosis of conventional lysosomes in several cell types. in ctls, the ca(2+)-regulated exocytosis of lytic granules/secretory lysosomes is responsible for the perforin/granzyme-mediated lysis of target cells. to investigate the role of syt vii in ctl effector function, the expression and function of syt vii were examined in wild-type and syt vii-deficient mice. in comparison with syt vii(+/+) controls, syt vii(-/-) animals were impaired ... | 2007 | 17237398 |
| suppression of the bacterial antigen-specific t cell response and the dendritic cell migration to the lymph nodes by osteopontin. | osteopontin (opn) has been reported to enhance the interferon (ifn)-gamma-producing th1-type t cell response through the induction of interleukin (il)-12 and the suppression of il-10. we therefore investigated whether opn could enhance th1 induction by vaccination against bacterial antigen in vivo. unexpectedly, the co-inoculation of opn suppressed the induction of ifn-gamma-producing cd4(+) t cells and t cell proliferative response after the subcutaneous heat-killed listeria monocytogenes(hklm) ... | 2007 | 17237609 |
| a novel surface autolysin of listeria monocytogenes serotype 4b, ispc, contains a 23-residue n-terminal signal peptide being processed in e. coli. | the 86-kda protein ispc of 774 amino acids in listeria monocytogenes serotype 4b has been recently identified as the target of humoral immune response to listerial infection and as a novel surface autolysin. a signal peptide is predicted at the n-terminal end of ispc, but no biochemical data has been shown to confirm the presence of the cleavage site of a signal peptidase. to address this and prepare sufficient amount of the protein for biochemical and structural characterization, we present a s ... | 2007 | 17239349 |
| fcgammariii-dependent inhibition of interferon-gamma responses mediates suppressive effects of intravenous immune globulin. | intravenous immune globulin (ivig) suppresses autoantibody-mediated inflammation by inducing and activating the inhibitory fc receptor fcgammariib and downstream negative signaling pathways. we investigated the effects of ivig on cellular responses to interferon-gamma (ifn-gamma), a potent macrophage activator that exacerbates inflammation. our study showed that ivig blocked ifn-gamma signaling and ifn-gamma-induced gene expression and suppressed ifn-gamma function in vivo during immune response ... | 2007 | 17239631 |
| exploring the performance of logistic regression model types on growth/no growth data of listeria monocytogenes. | several model types have already been developed to describe the boundary between growth and no growth conditions. in this article two types were thoroughly studied and compared, namely (i) the ordinary (linear) logistic regression model, i.e., with a polynomial on the right-hand side of the model equation (type i) and (ii) the (nonlinear) logistic regression model derived from a square root-type kinetic model (type ii). the examination was carried out on the basis of the data described in vermeu ... | 2007 | 17239980 |
| upregulation of vascular endothelial growth factor by heat-killed listeria monocytogenes in macrophages. | we investigated the effect of heat-killed listeria monocytogenes (hklm) on the expression of vascular endothelial growth factor (vegf) in raw264.7 macrophage-like cells. the expression of vegf was induced in raw264.7 cells treated with hklm. pretreatment of cells with cycloheximide, a protein synthesis inhibitor, inhibited the induction of vegf mrna by hklm. induction of vegf by hklm was partially inhibited by treatment of cells with sb203580, a p38 mitogen-activated protein kinase (mapk) inhibi ... | 2007 | 17240354 |
| genotypic and phenotypic diversity of lactococcus lactis isolates from batzos, a greek pdo raw goat milk cheese. | the genotypic and phenotypic variability of 40 lactococcus lactis isolates obtained from three cheese-making trials of batzos cheese made one in each, winter, spring and summer was investigated. rapd-pcr, plasmid profiling and pfge were used to study the genetic variability and distinguish closely related isolates. results showed a high degree of heterogeneity among strains. according to pfge data, all strains except one were clustered together (at a similarity level of approximately 50%) with t ... | 2007 | 17241681 |
| a pair of 9s: it's in the cards. | new work shows that card9, the recently identified member of the caspase recruitment domain-containing family, directly interacts with the cytoplasmic protein nod2 in the detection of intracellular bacteria. | 2007 | 17242682 |
| reduction in the incidence of invasive listeriosis in foodborne diseases active surveillance network sites, 1996-2003. | listeriosis is a leading cause of death among patients with foodborne diseases in the united states. monitoring disease incidence is an important element of listeriosis surveillance and control. | 2007 | 17243053 |
| listeria monocytogenes infection from foods prepared in a commercial establishment: a case-control study of potential sources of sporadic illness in the united states. | listeria monocytogenes has been estimated to cause >2500 illnesses and 500 deaths annually in the united states. efforts to reduce foodborne listeriosis have focused on foods frequently implicated in outbreaks. potential sources for l. monocytogenes infection not associated with outbreaks remain poorly understood. | 2007 | 17243054 |
| what can we do to prevent listeriosis in 2006? | 2007 | 17243055 | |
| cytosolic expression of seca2 is a prerequisite for long-term protective immunity. | induction of efficient adaptive t cell-mediated immunity against the intracellular bacterium listeria monocytogenes requires its successful invasion of host cell cytosol. however, it is not clear whether its cytosolic escape and growth are sufficient to induce t cell-mediated clearance and protection upon secondary infection. to investigate this issue, we have searched for mutants that do not induce long-term protective immunity yet invade the cytosol of infected cells. we found that mice immuni ... | 2007 | 17244189 |
| differential susceptibility of bone marrow-derived dendritic cells and macrophages to productive infection with listeria monocytogenes. | dendritic cells (dc) are required for the immune response against listeria monocytogenes and are permissive for infection in vivo and in vitro. however, it is unclear if dc provide a desirable intracellular niche for bacterial growth. to address this issue, we have compared the behaviour of l. monocytogenes in murine bone marrow-derived dc and macrophages (bmm). similar to bmm, bacteria escaped to the cytosol in dc, replicated, and spread to adjacent cells. however, dc infection was less robust ... | 2007 | 17250592 |