Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| neutrophil interactions stimulate evasive hyphal branching by aspergillus fumigatus. | invasive aspergillosis (ia), primarily caused by aspergillus fumigatus, is an opportunistic fungal infection predominantly affecting immunocompromised and neutropenic patients that is difficult to treat and results in high mortality. investigations of neutrophil-hypha interaction in vitro and in animal models of ia are limited by lack of temporal and spatial control over interactions. this study presents a new approach for studying neutrophil-hypha interaction at single cell resolution over time ... | 2017 | 28076396 |
| synergistic effects of tetrandrine with posaconazole against aspergillus fumigatus. | in our earlier in vitro and in vivo studies, synergistic effects were observed when itraconazole or voriconazole were combined with tetrandrine (tet) against aspergillus fumigatus, and the synergistic mechanism was related to inhibition of the drug efflux pump. posaconazole (pcz) is a broad-spectrum triazole antifungal agent used for the treatment of diverse fungal infections, including aspergillosis and candidiasis. herein, the antifungal effects of tet are further investigated in vitro and in ... | 2017 | 28080217 |
| aerobic microflora and endoparasites of freshly shot wild agouti (dasyprocta leporina) in trinidad, west indies. | the agouti ( dasyprocta leporina ) is a new world wild rodent hunted for its meat in trinidad and other latin american countries. studies on agouti under captive conditions have yielded some data on health-related aspects, but relatively very little is known about their wild counterparts. the environment of the agouti can influence the microflora and parasites harbored by the animals, which may contain zoonotic pathogens. here, the microflora found on the nasal mucosa and sections of the intesti ... | 2016 | 28080916 |
| clinical implications of globally emerging azole resistance in aspergillus fumigatus. | aspergillus fungi are the cause of an array of diseases affecting humans, animals and plants. the triazole antifungal agents itraconazole, voriconazole, isavuconazole and posaconazole are treatment options against diseases caused by aspergillus however, resistance to azoles has recently emerged as a new therapeutic challenge in six continents. although de novo azole resistance occurs occasionally in patients during azole therapy, the main burden is the aquisition of resistance through the enviro ... | 2016 | 28080986 |
| secondary metabolite arsenal of an opportunistic pathogenic fungus. | aspergillus fumigatus is a versatile fungus able to successfully exploit diverse environments from mammalian lungs to agricultural waste products. among its many fitness attributes are dozens of genetic loci containing biosynthetic gene clusters (bgcs) producing bioactive small molecules (often referred to as secondary metabolites or natural products) that provide growth advantages to the fungus dependent on environment. here we summarize the current knowledge of these bgcs-18 of which can be na ... | 2016 | 28080993 |
| first itraconazole resistant aspergillus fumigatus clinical isolate harbouring a g54e substitution in cyp51ap in south america. | a 27-year-old male rural worker was admitted with a fungal keratitis due to an injury involving plant detritus. | 2017 | 28087293 |
| isolation and identification of phyllospheric bacteria possessing antimicrobial activity from astragalus obtusifolius, prosopis juliflora, xanthium strumarium and hippocrepis unisiliqousa. | the widespread utilization of antimicrobial compounds has caused emergence of resistant microorganisms in the world. hence, the research to probe the products with antimicrobial features has led to finding natural habitats and discovering new pharmaceutical products. | 2017 | 28090278 |
| differential effects of vitamins a and d on the transcriptional landscape of human monocytes during infection. | vitamin a and vitamin d are essential nutrients with a wide range of pleiotropic effects in humans. beyond their well-documented roles in cellular differentiation, embryogenesis, tissue maintenance and bone/calcium homeostasis, both vitamins have attracted considerable attention due to their association with-immunological traits. nevertheless, our knowledge of their immunomodulatory potential during infection is restricted to single gene-centric studies, which do not reflect the complexity of im ... | 2017 | 28094291 |
| central role of il-23 and il-17 producing eosinophils as immunomodulatory effector cells in acute pulmonary aspergillosis and allergic asthma. | aspergillus fumigatus causes invasive pulmonary disease in immunocompromised hosts and allergic asthma in atopic individuals. we studied the contribution of lung eosinophils to these fungal diseases. by in vivo intracellular cytokine staining and confocal microscopy, we observed that eosinophils act as local sources of il-23 and il-17. remarkably, mice lacking eosinophils had a >95% reduction in the percentage of lung il-23p19+ cells as well as markedly reduced il-23 heterodimer in lung lavage f ... | 2017 | 28095479 |
| concentrations and diversity of microbes from four local bioaerosol emission sources in finland. | microbial particles can readily be released into the air from different types of man-made sources such as waste operations. microbiological emissions from different biological sources and their dispersion may be an issue of concern for area planning and for nearby residents. this study was designed to determine the concentrations and diversity of microbiological emissions from four different man-made source environments: waste center with composting windrows, sewage treatment plant, farming envi ... | 2011 | 28097955 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid identification of fungal rhinosinusitis pathogens. | filamentous fungi are among the most important pathogens, causing fungal rhinosinusitis (frs). current laboratory diagnosis of frs pathogens mainly relies on phenotypic identification by culture and microscopic examination, which is time consuming and expertise dependent. although matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms has been employed to identify various fungi, its efficacy in the identification of frs fungi is less clear. | 2017 | 28100314 |
| etv5 regulates il-10 production in th cells. | il-10 is an immunoregulatory cytokine that has broad effects across the immune system. in th cell subsets, th2 cells produce considerable amounts of il-10. the transcription factors that regulate il-10 production in th2 cells are still incompletely described. in this study, we demonstrate that the ets family transcription factor ets variant (etv)5 regulates il-10 production in th2 cells. t cell-specific etv5-deficient and littermate control mice demonstrated that il-10 production and gene expres ... | 2017 | 28100679 |
| sputum inflammatory mediators are increased in aspergillus fumigatus culture-positive asthmatics. | aspergillus fumigatus sensitization and culture in asthma are associated with disease severity and lung function impairment, but their relationship with airway inflammation is poorly understood. we investigated the profile of 24 sputum inflammatory mediators in a. fumigatus culture-positive or-negative moderate-to-severe asthmatics. fifty-two subjects were recruited from a single center. a. fumigatus was cultured from 19 asthmatics. asthma control, symptom score, lung function, and sputum cell c ... | 2017 | 28102063 |
| rna interference mediated knockdown of brugia malayi udp-galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae. | galactofuranose is an essential cell surface component present in bacteria, fungi and several nematodes such as caenorhabditis spp., brugia spp., onchocerca spp. and strongyloides spp. this sugar maintains the integrity of parasite surface and is essential for virulence. udp-galactopyranose mutase (bmugm) plays a key role in galf biosynthesis by catalyzing conversion of udp-galactopyranose into udp-galactofuranose and knockout studies of the gene in leishmania major, mycobacterium and aspergillu ... | 2017 | 28103957 |
| renal allograft aspergillus infection presenting with obstructive uropathy: a case report. | isolated renal allograft aspergillosis is rare and usually presents with fever and decreased glomerular filtration rate. presentation with obstructive uropathy caused by aspergillus fungal balls is much less common. we report a young male patient who presented with obstructive uropathy secondary to isolated renal allograft aspergillus infection 6 weeks after transplant. he was treated with nephrectomy and antifungal medications. | 2017 | 28104135 |
| first clinical isolation report of azole-resistant aspergillus fumigatus with tr34/l98h-type mutation in japan. | recently, azole-resistant aspergillus fumigatus containing a 34-bp or 46-bp tandem repeat in the promoter region of cyp51a combined with amino acid substitution(s) has appeared in the environment worldwide, including several asian countries. in this study, we isolated the 34-bp tandem repeat-containing azole-resistant a. fumigatus strain okh50 from a patient in japan in may 2016. the patient had not been treated with medical azoles before the strain isolation, suggesting that the resistant prope ... | 2017 | 28109740 |
| biomedical and catalytic applications of gold and silver-gold alloy nanoparticles biosynthesized using cell-free extract of bacillus safensis lau 13: antifungal, dye degradation, anti-coagulant and thrombolytic activities. | this study investigated the green biosynthesis of gold (au) and silver-gold alloy (ag-au) nanoparticles using cell-free extract of bacillus safensis lau 13 strain (genbank accession no: kj461434). the biosynthesized aunps and ag-aunps were characterized using uv-vis spectroscopy, fourier-transform infrared spectroscopy, and transmission electron microscopy. evaluation of the antifungal activities, degradation of malachite green, anti-coagulation of blood and thrombolysis of human blood clot by t ... | 2016 | 28113560 |
| cipc is important for aspergillus fumigatus virulence. | aspergillus fumigatus is the main causative agent of invasive aspergillosis, a disease that affects immunocompromised patients and has a high mortality rate. we previously observed that the transcription of a cipc-like gene was increased when a. fumigatus encountered an increased co2 concentration, as occurs during the infection process. cipc is a protein of unknown function that might be associated with fungal pathogenicity. in this study, the cipc gene was disrupted in a. fumigatus to evaluate ... | 2017 | 28120495 |
| [accurate diagnosis of pseudomonas luteola in routine microbiology laboratory: on the occasion of two isolates]. | pseudomonas luteola which was previously known as chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. it is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. it has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. thus, early and accurate ide ... | 2016 | 28124968 |
| release of type 2 cytokines by epithelial cells of nasal polyps. | background. t2 inflammation of chronic rhinosinusitis with nasal polyps (crswnp) may be influenced by epithelial cytokines release (tslp, il-25, and il-33). we investigated the release of tslp, il-25, and il-33 by epithelial crswnp cells compared to epithelial sinus mucosa cells of patients with chronic rhinosinusitis without nasal polyps (crssnp). methods. il-25, il-33, and tslp were measured by elisa in the supernatant of cell cultures derived by crswnp (9 patients, 6 atopic) and crssnp (7 pat ... | 2016 | 28127565 |
| conserved cysteine residues determine substrate specificity in a novel as(iii) s-adenosylmethionine methyltransferase from aspergillus fumigatus. | methylation of inorganic arsenic is a central process in the organoarsenical biogeochemical cycle. members of every kingdom have arsm as(iii) s-adenosylmethionine (sam) methyltransferases that methylates inorganic as(iii) into mono- (mas(iii)), di- (dmas(iii)) and tri- (tmas(iii)) methylarsenicals. every characterized arsm to date has four conserved cysteine residues. all four cysteines are required for methylation of as(iii) to mas(iii), but methylation of mas(iii) to dmas(iii) requires only th ... | 2017 | 28127843 |
| household proximity to water and nontuberculous mycobacteria in children with cystic fibrosis. | nontuberculous mycobacteria (ntm) have a particular affinity for patients with cystic fibrosis (cf). recent studies suggest a possible relationship between acquiring ntm and the level of environmental water in a given area. we sought to determine if there is an association between household proximity to water and ntm in children with cf. | 2017 | 28135043 |
| the role of mincle in innate immune to fungal keratitis. | this study aimed to detect the early expression of macrophage-inducible c-type lectin (mincle) and investigate its role in the innate immune response of fungal keratitis (fk). | 2017 | 28141595 |
| broad spectrum anti-infective properties of benzisothiazolones and the parallels in their anti-bacterial and anti-fungal effects. | various mono- and bis-benzisothiazolone derivatives were synthesized and screened against different strains of bacteria and fungi in order to understand the effect of multiple electrophilic sulfur atoms and substitution pattern in the immediate vicinity of reactive sulfur. staphyllococcus aureus-atcc 7000699, mrsa and s. aureus-atcc 29213 (quality control strain) were more susceptible to this class of compounds, and the most potent derivative 1.15 had mic50 of 0.4μg/ml (cf. gentamicin=0.78μg/ml) ... | 2017 | 28159413 |
| ram1 gene, encoding a subunit of farnesyltransferase, contributes to growth, antifungal susceptibility to amphotericin b of aspergillus fumigatus. | 2017 | 28159997 | |
| structure of the sliding clamp from the fungal pathogen aspergillus fumigatus (afumpcna) and interactions with human p21. | the fungal pathogen aspergillus fumigatus has been implicated in a drastic increase in life-threatening infections over the past decade. however, compared to other microbial pathogens, little is known about the essential molecular processes of this organism. one such fundamental process is dna replication. the protein responsible for ensuring processive dna replication is pcna (proliferating cell nuclear antigen, also known as the sliding clamp), which clamps the replicative polymerase to dna. h ... | 2017 | 28165677 |
| global population genetic analysis of aspergillus fumigatus. | aspergillus fumigatus is a ubiquitous opportunistic fungal pathogen capable of causing invasive aspergillosis, a globally distributed disease with a mortality rate of up to 90% in high-risk populations. effective control and prevention of this disease require a thorough understanding of its epidemiology. however, despite significant efforts, the global molecular epidemiology of a. fumigatus remains poorly understood. in this study, we analyzed 2,026 a. fumigatus isolates from 13 countries in fou ... | 2017 | 28168221 |
| a novel phosphoregulatory switch controls the activity and function of the major catalytic subunit of protein kinase a in aspergillus fumigatus. | invasive aspergillosis (ia), caused by the filamentous fungal pathogen aspergillus fumigatus, is a major cause of death among immunocompromised patients. the cyclic amp/protein kinase a (pka) signaling pathway is essential for hyphal growth and virulence of a. fumigatus, but the mechanism of regulation of pka remains largely unknown. here, we discovered a novel mechanism for the regulation of pka activity in a. fumigatus via phosphorylation of key residues within the major catalytic subunit, pka ... | 2017 | 28174315 |
| [pulmonary co-infection due to nocardia cyriacigeorgica and aspergillus fumigatus]. | pulmonary nocardiosis is an uncommon pulmonary infection caused by aerobic gram-positive bacteria of the genus nocardia. nocardia sp. are environmental organisms spread worldwide. approximately 50 nocardia species have been described to date, about 30 of which are known to cause human disease. nocardia cyriacigeorgica was first reported in 2001. | 2017 | 28176520 |
| prospective multicenter german study on pulmonary colonization with scedosporium /lomentospora species in cystic fibrosis: epidemiology and new association factors. | an increasing rate of respiratory colonization and infection in cystic fibrosis (cf) is caused by fungi of the scedosporium apiospermum species complex or lomentospora prolificans (sac-lp). these fungi rank second among the filamentous fungi colonizing the cf airways, after aspergillus fumigatus. however, the epidemiology, clinical relevance and risk of pulmonary colonization with sac-lp are rarely understood in cf. the objective of the present prospective multicenter study was to study pathogen ... | 2017 | 28178337 |
| synthetic cystic fibrosis sputum medium diminishes <i>burkholderia cenocepacia</i> antifungal activity against <i>aspergillus fumigatus</i> independently of phenylacetic acid production. | phenylacetic acid (paa), an intermediate of phenylalanine degradation, is emerging as a signal molecule in microbial interactions with the host. in this work, we explore the presence of phenylalanine and paa catabolism in three microbial pathogens of the cystic fibrosis (cf) lung microbiome: <i>pseudomonas aeruginosa</i>, <i>burkholderia cenocepacia</i> and <i>aspergillus fumigatus</i>. while <i>in silico</i> analysis of <i>b. cenocepacia</i> j2315 and <i>a. fumigatus</i> af293 genome sequences ... | 2017 | 28178425 |
| structural, mechanistic and functional insight into gliotoxin bis-thiomethylation in aspergillus fumigatus. | gliotoxin is an epipolythiodioxopiperazine (etp) class toxin, contains a disulfide bridge that mediates its toxic effects via redox cycling and is produced by the opportunistic fungal pathogen aspergillus fumigatus self-resistance against gliotoxin is effected by the gliotoxin oxidase glit, and attenuation of gliotoxin biosynthesis is catalysed by gliotoxin s-methyltransferase gtma. here we describe the x-ray crystal structures of gtma-apo (1.66 å), gtma complexed to s-adenosylhomocysteine (1.33 ... | 2017 | 28179499 |
| terbinafine-loaded wound dressing for chronic superficial fungal infections. | in spite of developing new drugs and modern formulations, the treatments of chronic fungal infections are still challenging. fibrous wound dressings are new suggestions for the treatment of chronic superficial infections. in the present study, we formulated an antifungal agent, terbinafine hydrochloride (tfh), which is a hydrophobic drug, in wound dressings prepared by electrospun polycaprolactone, polycaprolactone/gelatin (50:50 w/w) and gelatin. to obtain more water-stable meshes, the preparat ... | 2017 | 28183589 |
| fatal disseminated rasamsonia infection in cystic fibrosis post-lung transplantation. | disseminated fungal infections are a known serious complication in individuals with cystic fibrosis (cf) following orthotopic lung transplantation. aspergillus fumigatus and scedosporium species are among the more common causes of invasive fungal infection in this population. however, it is also important for clinicians to be aware of other emerging fungal species which may require markedly different antifungal therapies. | 2017 | 28185887 |
| role of n-linked glycosylation in the enzymatic properties of a thermophilic gh 10 xylanase from aspergillus fumigatus expressed in pichia pastoris. | n-glycosylation is a posttranslational modification commonly occurred in fungi and plays roles in a variety of enzyme functions. in this study, a xylanase (af-xyna) of glycoside hydrolase (gh) family 10 from aspergillus fumigatus harboring three potential n-glycosylation sites (n87, n124 and n335) was heterologously produced in pichia pastoris. the n-glycosylated af-xyna (wt) exhibited favorable temperature and ph optima (75°c and ph 5.0) and good thermostability (maintaining stable at 60°c). to ... | 2017 | 28187141 |
| [prevalence of sensitization to inhalant allergens in the immunology department from hospital de especialidades pediátricas maría, honduras, 2016]. | allergic diseases are a public health problem; estimates indicate that between 30% and 40% of the world population is affected by some allergy. knowing the prevalence of allergen sensitization allows for adequate diagnoses and treatments to be offered. in honduras there are no studies available in pediatric patients. | 2017 | 28188710 |
| chemical characteristics, antimicrobial and cytotoxic activities of the essential oil of egyptian cinnamomum glanduliferum bark. | the essential oil isolated from the bark of cinnamomum glanduliferum (wall) meissn that grown in egypt was screened for the first time. the chemical composition was analyzed by gc and gc/mass. the antimicrobial activity of the oil was assessed using agar-well diffusion method toward representatives for each of gram-positive bacteria, gram-negative bacteria, and fungi. the cytotoxic activity was checked using three human cancer cell lines. twenty seven compounds were identified, representing 99.0 ... | 2017 | 28190283 |
| maternal house dust mite exposure during pregnancy enhances severity of house dust mite-induced asthma in murine offspring. | atopic status of the mother and maternal exposure to environmental factors are associated with increased asthma risk. moreover, animal models demonstrate that exposure to allergens in strongly sensitized mothers influences offspring asthma development, suggesting that in utero exposures can influence offspring asthma. however, it is unclear whether maternal exposure to common human allergens such as house dust mite (hdm), in the absence of additional adjuvants, influences offspring asthma develo ... | 2017 | 28192144 |
| first isolation of the pan-azole-resistant aspergillus fumigatus cyp51a tr46/y121f/t289a mutant in a uk patient. | 2017 | 28193463 | |
| mitochondrial camkii inhibition in airway epithelium protects against allergic asthma. | excessive ros promote allergic asthma, a condition characterized by airway inflammation, eosinophilic inflammation, and increased airway hyperreactivity (ahr). the mechanisms by which airway ros are increased and the relationship between increased airway ros and disease phenotypes are incompletely defined. mitochondria are an important source of cellular ros production, and our group discovered that ca(2+)/calmodulin-dependent protein kinase ii (camkii) is present in mitochondria and activated b ... | 2017 | 28194433 |
| sybr safe(tm) efficiently replaces ethidium bromide in aspergillus fumigatus gene disruption. | invasive aspergillosis is a disease responsible for high mortality rates, caused mainly by aspergillus fumigatus. the available drugs are limited and this disease continues to occur at an unacceptable frequency. gene disruption is essential in the search for new drug targets. an efficient protocol for a. fumigatus gene disruption was described but it requires ethidium bromide, a genotoxic agent, for dna staining. therefore, the present study tested sybr safe(tm), a non-genotoxic dna stain, in a. ... | 2017 | 28198513 |
| regulation of sterol biosynthesis in the human fungal pathogen aspergillus fumigatus: opportunities for therapeutic development. | sterols are a major component of eukaryotic cell membranes. for human fungal infections caused by the filamentous fungus aspergillus fumigatus, antifungal drugs that target sterol biosynthesis and/or function remain the standard of care. yet, an understanding of a. fumigatus sterol biosynthesis regulatory mechanisms remains an under developed therapeutic target. the critical role of sterol biosynthesis regulation and its interactions with clinically relevant azole drugs is highlighted by the bas ... | 2017 | 28203225 |
| epigenetic modifier induced enhancement of fumiquinazoline c production in aspergillus fumigatus (ga-l7): an endophytic fungus from grewia asiatica l. | present study relates to the effect of valproic acid, an epigenetic modifier on the metabolic profile of aspergillus fumigatus (ga-l7), an endophytic fungus isolated from grewia asiatica l. seven secondary metabolites were isolated from a. fumigatus (ga-l7) which were identified as: pseurotin a, pseurotin d, pseurotin f2, fumagillin, tryprostatin c, gliotoxin and bis(methylthio)gliotoxin. addition of valproic acid in the growth medium resulted in the alteration of secondary metabolic profile wit ... | 2017 | 28213885 |
| transcription factor afmac1 controls copper import machinery in aspergillus fumigatus. | copper (cu) is an essential metal for all living organisms, although it is toxic in excess. filamentous fungus must acquire copper from its environment for growth. despite its essentiality for growth, the mechanisms that maintain copper homeostasis are not fully understood in filamentous fungus. to gain insights into copper homeostasis, we investigated the roles of a copper transcription factor afmac1 in the life-threatening fungus aspergillus fumigatus, a homolog of the yeast mac1. we observed ... | 2017 | 28215034 |
| iron acquisition in fungal pathogens of humans. | the devastating infections that fungal pathogens cause in humans are underappreciated relative to viral, bacterial and parasitic diseases. in recent years, the contributions to virulence of reductive iron uptake, siderophore-mediated uptake and heme acquisition have been identified in the best studied and most life-threatening fungal pathogens: candida albicans, cryptococcus neoformans and aspergillus fumigatus. in particular, exciting new work illustrates the importance of iron acquisition from ... | 2017 | 28217776 |
| thermophilic fungi to dominate aflatoxigenic/mycotoxigenic fungi on food under global warming. | certain filamentous fungi produce mycotoxins that contaminate food. mycotoxin contamination of crops is highly influenced by environmental conditions and is already affected by global warming, where there is a succession of mycotoxigenic fungi towards those that have higher optimal growth temperatures. aflatoxigenic fungi are at the highest limit of temperature although predicted increases in temperature are beyond that constraint. the present paper discusses what will succeed these fungi and re ... | 2017 | 28218685 |
| bactericidal and fungicidal activity of n-chlorotaurine is enhanced in cystic fibrosis sputum medium. | lung infections with multiresistant pathogens are a major problem of patients suffering from cystic fibrosis (cf). n-chlorotaurine (nct), a microbicidal active chlorine compound with no resistance development, is well tolerated upon inhalation. the aim of this study was to investigate nct on its bactericidal and fungicidal activity in vitro in artificial sputum medium (asm) mimicking the composition of cystic fibrosis mucus.the medium was inoculated with bacteria (staphylococcus aureus including ... | 2017 | 28223376 |
| in vitro and in vivo antifungal profile of a novel and long acting inhaled azole, pc945, on aspergillus fumigatus infection. | the profile of pc945, a novel triazole antifungal, designed for administration via inhalation, has been assessed in a range of in vitro and in vivo studies. pc945 was characterized as a potent, tight-binding inhibitor of aspergillus fumigatus sterol 14α-demethylase (cyp51a and cyp51b) activity (ic50, 0.23 μm and 0.22 μm, respectively), with characteristic type ii azole binding spectra. against 96 clinically isolated a. fumigatus strains, the mic values of pc945 ranged from 0.032∼>8 μg/ml, whilst ... | 2017 | 28223388 |
| heterogeneity confounds establishment of "a" model microbial strain. | aspergillus fumigatus is a ubiquitous environmental mold and the leading cause of diverse human diseases ranging from allergenic bronchopulmonary aspergillosis (abpa) to invasive pulmonary aspergillosis (ipa). experimental investigations of the biology and virulence of this opportunistic pathogen have historically used a few type strains; however, it is increasingly observed with this fungus that heterogeneity among isolates potentially confounds the use of these reference isolates. illustrating ... | 2017 | 28223452 |
| α-bisabolol inhibits aspergillus fumigatus af239 growth via affecting microsomal ∆(24)-sterol methyltransferase as a crucial enzyme in ergosterol biosynthesis pathway. | finding new compounds with antifungal properties is an important task due to the side effects of common antifungal drugs and emerging antifungal resistance in fungal strains. ∆(24)-sterol methyltransferase (24-smt) is a crucial enzyme that plays important roles in fungal ergosterol biosynthesis pathway and is not found in humans. in the present study, the effects of α-bisabolol on aspergillus fumigatus af239 growth and ergosterol synthesis on the base of 24-smt enzyme activity were studied; in a ... | 2017 | 28224386 |
| invasive pulmonary aspergillosis in an immunocompromised patient with severe ulcerative colitis. | inflammatory bowel disease patients at risk of opportunistic infections are those treated with immunomodulators. toruner and colleagues found that corticosteroid use was more commonly associated with fungal infections. we present a case of invasive pulmonary aspergillosis due to corticosteroid therapy in the context of a severe ulcerative colitis flare. we want to highlight the relationship between aspergillus fumigatus and horses; and the use of leucocitapheresis combined with mesalazine as an ... | 2017 | 28229616 |
| innate lung defense during invasive aspergillosis: new mechanisms. | invasive aspergillosis (ia) is one of the most difficult to treat and, consequently, one of the most lethal fungal infections known to man. continued use of immunosuppressive agents during chemotherapy and organ transplantation often leads to the development of neutropenia, the primary risk factor for ia. however, ia is also becoming more appreciated in chronic diseases associated with corticosteroid therapy. the innate immune response to aspergillus fumigatus, the primary agent in ia, plays a p ... | 2017 | 28231567 |
| [infections in cystic fibrosis: up-to-date]. | this review focused on the news in cf airways infection. international guidelines were provided for the care of non tuberculous mycobacteria, and recent studies stressed on the benefit effect of azithromycin or combined antibiotics. the identification of multiresistant environmental bacteria in airways made to account for little-known consequences. early diagnosis and eradication of pseudomonas aeruginosa and staphylococcus aureus methi-r were still a concern, and reports were proposed. however, ... | 2016 | 28231891 |
| subacute invasive pulmonary aspergillosis as a rare cause of pneumothorax in immunocompetent patient: brief report. | subacute invasive pulmonary aspergillosis (ipa) represents a form of chronic pulmonary aspergillosis which affects immunocompetent individuals or mildly immunocompromised persons with underlying pulmonary disease. pneumothorax can be a rare complication of subacute ipa due to a leakage of air from an air-filled lung cavitation into the pleural space. herein, we report rare and unusual case of pneumothorax in a patient with pulmonary cavity infection. a 40-year-old woman was admitted to thoracic ... | 2017 | 28233110 |
| triazole resistance in aspergillus species: an emerging problem. | aspergillus species are ubiquitous fungal saprophytes found in diverse ecological niches worldwide. among them, aspergillus fumigatus is the most prevalent and is largely responsible for the increased incidence of invasive aspergillosis with high mortality rates in some immunocompromised hosts. azoles are the first-line drugs in treating diseases caused by aspergillus spp. however, increasing reports in a. fumigatus azole resistance, both in the clinical setting and in the environment, are threa ... | 2017 | 28236169 |
| aryl-alkyl-lysines: membrane-active fungicides that act against biofilms of candida albicans. | mortality due to pathogenic fungi has been exacerbated by the rapid development of resistance to frontline antifungal drugs. fungicidal compounds with novel mechanisms of action are urgently needed. aryl-alkyl-lysines, which are membrane-active small molecules, were earlier shown to be broad-spectrum antibacterial agents with potency in vitro and in vivo. herein, we report the antifungal properties of aryl-alkyl-lysines. after identifying the most active compound (nck-10), we tested its activity ... | 2017 | 28238268 |
| caspofungin exposure alters the core septin aspb interactome of aspergillus fumigatus. | aspergillus fumigatus, the main etiological agent of invasive aspergillosis, is a leading cause of death in immunocompromised patients. septins, a conserved family of gtp-binding proteins, serve as scaffolding proteins to recruit enzymes and key regulators to different cellular compartments. deletion of the a. fumigatus septin aspb increases susceptibility to the echinocandin antifungal caspofungin. however, how aspb mediates this response to caspofungin is unknown. here, we characterized the as ... | 2017 | 28238781 |
| infections in severe alcoholic hepatitis. | severe alcoholic hepatitis (sah), defined by a modified discriminant function ≥32, is the most severe form of alcohol-induced liver disease and is associated with a 1-month mortality rate of around 30%. corticosteroid treatment remains the only therapeutic option that improves short-term survival. infectious complications, occurring in approximately 50% of patients, are the main causes of death, even in patients who benefit from corticosteroids. liver failure, recent alcohol consumption and immu ... | 2017 | 28243035 |
| challenges and strategies for proteome analysis of the interaction of human pathogenic fungi with host immune cells. | opportunistic human pathogenic fungi including the saprotrophic mold aspergillus fumigatus and the human commensal candida albicans can cause severe fungal infections in immunocompromised or critically ill patients. the first line of defense against opportunistic fungal pathogens is the innate immune system. phagocytes such as macrophages, neutrophils and dendritic cells are an important pillar of the innate immune response and have evolved versatile defense strategies against microbial pathogen ... | 2015 | 28248281 |
| resistance of aspergillus fumigatus to micafungin is increased by exogenous β-glucan. | aspergillus species are ubiquitous in the environment and aspergillus fumigatus can cause life-threatening infections in immunocompromised patients. β-1,3-/1,6-glucan is a major fungal cell wall polysaccharide that has various biological effects on the infected host, but little is known about the influence of β-glucan on the fungus itself. in a previous study, we demonstrated that the cell wall β-glucan content could be increased in aspergillus spp. by addition of β-glucan to the culture medium. ... | 2017 | 28250363 |
| insights into the unprecedented epoxidation mechanism of fumitremorgin b endoperoxidase (ftmox1) from aspergillus fumigatus by qm/mm calculations. | fumitremorgin b endoperoxidase (ftmox1) from aspergillus fumigatus is the first reported α-ketoglutarate-dependent mononuclear non-haem iron enzyme that catalyzes the endoperoxide formation reaction, converting fumitremorgin b to verruculogen. experiments reveal that the molecular oxygen (o2) is incorporated into verruculogen without o-o bond scission, which differs from the currently known non-haem iron enzymes, but the mechanistic details are still unclear. in this paper, on the basis of the c ... | 2017 | 28256663 |
| culture-positive pulmonary aspergillosis infection: clinical and laboratory features of solid-organ transplant recipients. | aspergillosis is a common fungal infection among solid-organ transplant recipients. even after awareness of this infection occurs, there are still gaps in nonculture diagnostic tests, which can delay treatment initiation. here, we aimed to define the common traits of pulmonary aspergillosis infection among solid-organ transplant recipients, thus shedding light on prevention and early diagnosis. | 2017 | 28260471 |
| post-influenza aspergillosis, do not underestimate influenza b. | our objective is to highlight and focus on post-influenza aspergillosis, triggered by influenza b virus. this relatively new clinical entity is often associated with a fulminant course of respiratory decline and high mortality. a 51-year immunocompetent woman, without any medical history or risk factors for developing a complicated influenza infection, was admitted to the intensive care unit. during admission, she presented with an afebrile flu-like syndrome, myocarditis, rhabdomyolysis, multipl ... | 2017 | 28260935 |
| diversity, phylogeny, anticancer and antimicrobial potential of fungal endophytes associated with monarda citriodora l. | present study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of the monarda citriodora cerv. ex lag. (lamiaceae/labiatae). anticancer and antimicrobial potential of isolated endophytes have also been investigated. | 2017 | 28264654 |
| contributions of both atp-binding cassette transporter and cyp51a proteins are essential for azole resistance in aspergillus fumigatus. | while azole drugs targeting the biosynthesis of ergosterol are effective antifungal agents, their extensive use has led to the development of resistant organisms. infections involving azole resistant forms of the filamentous fungus aspergillus fumigatus are often associated with genetic changes in the cyp51a gene encoding the lanosterol α14 demethylase target enzyme. both a sequence duplication in the cyp51a promoter (tr34) as well as a substitution mutation in the coding sequence (l98h) are req ... | 2017 | 28264842 |
| specific and novel micrornas are regulated as response to fungal infection in human dendritic cells. | within the last two decades, the incidence of invasive fungal infections has been significantly increased. they are characterized by high mortality rates and are often caused by candida albicans and aspergillus fumigatus. the increasing number of infections underlines the necessity for additional anti-fungal therapies, which require extended knowledge of gene regulations during fungal infection. micrornas are regulators of important cellular processes, including the immune response. by analyzing ... | 2017 | 28280489 |
| lah is a transmembrane protein and requires spa10 for stable positioning of woronin bodies at the septal pore of aspergillus fumigatus. | woronin bodies are specialized, fungal-specific organelles that enable an immediate closure of septal pores after injury to protect hyphae from excessive cytoplasmic bleeding. in most ascomycetes, woronin bodies are tethered at the septal pore by so-called lah proteins. using the pathogenic mold aspergillus fumigatus as a model organism, we show that the c-terminal 288 amino acids of lah (lahc288) bind to the rim of the septal pore. lahc288 essentially consists of a membrane spanning region and ... | 2017 | 28281662 |
| establishment of crispr/cas9 in alternaria alternata. | the filamentous fungus alternaria alternata is a potent producer of many secondary metabolites, some of which like alternariol or alternariol-methyl ether are toxic and/or cancerogenic. many alternaria species do not only cause post-harvest losses of food and feed, but are aggressive plant pathogens. despite the great economic importance and the large number of research groups working with the fungus, the molecular toolbox is rather underdeveloped. gene deletions often result in heterokaryotic s ... | 2017 | 28286319 |
| two new sesquiterpene lactone glycosides from cnicus benedictus. | two new sesquiterpene lactone glycosides, namely melitensin 15-o-β-d-glucoside (1) and 11β,13-dihydrosalonitenolide 15-o-β-d-glucoside (2), along with eight known compounds (3-10) were isolated from the aerial part of cnicus benedictus l. their structures were elucidated from analyses of extensive spectroscopic data. compounds 1-6 all possessed an α-methyl-γ-lactone moiety. moreover, compound 5 exhibited moderate activity against the growth of aspergillus fumigatus, with ic50 values of 17.67 μg ... | 2017 | 28287272 |
| epidemiology of multidrug-resistant gram-negative pathogens isolated from ventilator-associated pneumonia in icu patients. | antibiotic resistance among gram-negative pathogens isolated from ventilator-associated pneumonia (vap) poses a grave threat in intensive care unit (icu) patients. the aim of this study was to assess the prevalence of pathogens in icu patients and their drug resistance profile. the prevalence of extended-spectrum β-lactamases (esbls), ampc β-lactamases and metallo-β-lactamases (mbls) was also assessed. | 2017 | 28288860 |
| [the regulative effects of aspergillus fumigatus on expression of glucocorticoid receptor in asthmatic rats]. | objective: to study the regulative effects of aspergillus fumigatus (a.fumigatus) on expression of glucocorticoid receptor (gcr) in asthmatic rats. methods: wistar rats were randomly divided into 4 groups: a normal control group (uc), a normal control with a. fumigatus group (uc+ af), an ova group (ova), and an ova with a. fumigatus group (ova+ af). ova and ova+ af groups were sensitized and challenged with ova to establish asthmatic models. uc and uc+ af groups were given normal saline as contr ... | 2017 | 28297812 |
| central role of the trehalose biosynthesis pathway in the pathogenesis of human fungal infections: opportunities and challenges for therapeutic development. | invasive fungal infections cause significant morbidity and mortality in part due to a limited antifungal drug arsenal. one therapeutic challenge faced by clinicians is the significant host toxicity associated with antifungal drugs. another challenge is the fungistatic mechanism of action of some drugs. consequently, the identification of fungus-specific drug targets essential for fitness in vivo remains a significant goal of medical mycology research. the trehalose biosynthetic pathway is found ... | 2017 | 28298477 |
| challenges in microbiological diagnosis of invasive aspergillus infections. | invasive aspergillosis (ia) has been increasingly reported in populations other than the historical hematology patients and there are new questions about the performance of microbiological tools. microscopy and culture have been completed by biomarkers, either antigens or dna, and in blood or respiratory specimens or both. first studied in hematology, the antigen galactomannan performance in serum is low in other patient populations where the pathophysiology of the infection can be different and ... | 2017 | 28299183 |
| aspergillosis, a natural infection in poultry: mycological and molecular characterization and determination of gliotoxin in aspergillus fumigatus isolates. | aspergillosis affects all types of birds; it causes the loss of specimens with high ecologic value and also leads to significant economic losses within the poultry industry. the main etiologic agent is aspergillus fumigatus , a filamentary fungus with multiple virulence factors, such as gliotoxin (gt), which is an immunosuppressive epipolythiodioxopiperazine molecule. necropsy was performed on 73 poultry from different provenances, all of which presented with a respiratory semiology compatible w ... | 2017 | 28301237 |
| triazole resistance in aspergillus fumigatus clinical isolates obtained in nanjing, china. | during the past decades, the incidence of invasive aspergillosis (ia) caused by aspergillus fumigatus has increased dramatically. the aims of this study were to investigate the susceptibility of clinical isolates of a. fumigatus to triazole and the underlying cyp51a mutations in triazole-resistant a. fumigatus. | 2017 | 28303848 |
| aspergillus pericarditis with tamponade in a renal transplant patient. | aspergillus pericarditis is a rare and life-threatening infection in immunosuppressed patients. it has nonspecific clinical manifestations that often mimic other disease entities especially in patients who have extensive comorbidities. diagnosis is oftentimes delayed and rarely done antemortem. a high degree of suspicion in immunocompromised patients is necessary for evaluation and timely diagnosis. this is a case of aspergillus pericarditis with cardiac tamponade in a renal transplant patient w ... | 2017 | 28316844 |
| characterization of a pa14 domain-containing galactofuranose-specific β-d-galactofuranosidase from streptomyces sp. | as a constituent of polysaccharides and glycoconjugates, β-d-galactofuranose (galf) exists in several pathogenic microorganisms. although we recently identified a β-d-galactofuranosidase (galf-ase) gene, orf1110, in the streptomyces strain jha19, very little is known about the galf-ase gene. here, we characterized a strain, named jha26, in the culture supernatant of which exhibited galf-ase activity for 4-nitrophenyl β-d-galactofuranoside (pnp-β-d-galf) as a substrate. draft genome sequencing of ... | 2017 | 28317475 |
| synthesis, characterization and antimicrobial activity of biguanidinylated chitosan-g-poly[(r)-3-hydroxybutyrate]. | chitosan biguanidine hydrochloride (chg) and low molecular weight poly[(r)-3-hydroxybutyrate] (phb) were successfully prepared to overcome the solubility problem of chitosan and phb and also to enhance antimicrobial activity of chitosan. the graft copolymers based on chg and phb (chg-grafted phb) were then prepared via condensation reaction of the carboxylic groups of phb with the amino groups of chg. these graft copolymers swell in water. the prepared graft copolymers were characterized by ftir ... | 2017 | 28322958 |
| evaluation of a real-time impedance analysis platform on fungal infection. | end-point assays of in vitro cell proliferation and death have been employed to study the mechanisms of fungal pathogenesis and have shown the responses of host cells at individual time points. a new cell analysis technology has been developed that allows for the continuous measurement and quantification of cell activities, thus enabling the dynamic assessment of electrical impedance when various pathogens are cultured in vitro. in this study, this system was evaluated to determine the response ... | 2017 | 28323067 |
| enzymatic production of glucosamine and chitooligosaccharides using newly isolated exo-β-d-glucosaminidase having transglycosylation activity. | exochitosanase secreting fungus (a. fumigatus iit-004) was isolated from fish waste using 1 % (w/v) chitosan as sole carbon source after multistage screening. chitosan-dependent exochitosanase enzyme production (6 iu ml(-1)) in log phase of growth (chitosan utilization rate 0.11 g g(-1) cell h(-1)) was observed for aspergillus fumigatus in chitosan minimal salt medium and there was no enzyme production in glucose medium. enzyme production was found to be extracellular and subjected to purificati ... | 2016 | 28330083 |
| paper wasp nest-mediated biosynthesis of silver nanoparticles for antimicrobial, catalytic, anticoagulant, and thrombolytic applications. | biosynthesis of silver nanoparticles (agnps) using nest extract of paper wasp (polistes sp) was investigated in this work. the agnps were characterized by uv-vis spectroscopy, fourier transform infrared spectroscopy (ftir), and transmission electron microscopy (tem), and evaluated for antibacterial, antifungal, dye degradation, blood anticoagulation, and blood clot dissolution (thrombolytic) activities. the crystalline polydispersed agnps with size range of 12.5-95.55 nm absorbed maximally at 42 ... | 2016 | 28330212 |
| purification and characterization of polygalacturonase from aspergillus fumigatus mtcc 2584 and elucidating its application in retting of crotalaria juncea fiber. | polygalacturonases represents an important member of pectinases group of enzymes with diverse industrial applications and is widely distributed among fungi, bacteria, yeasts, plants and some plant parasitic nematodes. an endo-polygalacturonase from a new fungal source aspergillus fumigatus mtcc 2584 was produced under solid-state fermentation conditions and was purified simply by acetone precipitation and gel-filtration chromatography technique. the approximate molecular weight of the purified p ... | 2016 | 28330273 |
| comparative evaluation of sensititre yeastone and clsi m38-a2 reference method for antifungal susceptibility testing of aspergillus spp. against echinocandins. | sensititre yeastone (yo) panels were assessed for in vitro susceptibility testing of echinocandins against 39 isolates of aspergillus fumigatus, a. flavus, and a. terreus, including two echinocandin-resistant a. fumigatus strains, using different inocula (10(3), 10(4), and 10(5) cfu/ml), incubation times (16 to 48 h), and endpoints (first blue or purple well) and compared to clsi m38-a2. the best agreement was found with an inoculum of 10(4) cfu/ml, incubation times of 20 h for a. flavus and of ... | 2017 | 28330883 |
| synthesis and biological evaluation of a new series of benzimidazole derivatives as antimicrobial, antiquorum-sensing and antitumor agents. | new benzimidazole derivatives were synthesized and assessed for antimicrobial efficacy toward escherichia coli, bacillus cereus, staphylococcus aureus, candida albicans and aspergillus fumigatus 293. results indicated that compounds 3c and 3n have promising activity toward s. aureus, whereas 3i exhibited remarkable efficacy toward b. cereus. moreover, compound 3c was proved to be the most active antifungal analog toward c. albicans. on the other hand, 3n displayed the highest activity against a. ... | 2017 | 28334654 |
| dirhamnolipids secreted from pseudomonas aeruginosa modify anjpegungal susceptibility of aspergillus fumigatus by inhibiting β1,3 glucan synthase activity. | pseudomonas aeruginosa and aspergillus fumigatus are the two microorganisms responsible for most of the chronic infections in cystic fibrosis patients. p. aeruginosa is known to produce quorum-sensing controlled rhamnolipids during chronic infections. here we show that the dirhamnolipids secreted from p. aeruginosa (i) induce a. fumigatus to produce an extracellular matrix, rich in galactosaminogalactan, 1,8-dihydroxynaphthalene (dhn)- and pyo-melanin, surrounding their hyphae, which facilitates ... | 2017 | 28338676 |
| detection of polish clinical aspergillus fumigatus isolates resistant to triazoles. | we studied the presence of triazole resistance of 121 aspergillus fumigatus clinical isolates collected in two polish cities, warsaw and wrocław, to determine if resistance is emerging in our country. we identified five itraconazole resistant isolates (4.13%) carrying the tr34/l98h alteration in cyp51a gene, four of which were cross-resistant to posaconazole and one to voriconazole. one isolate was intermediate susceptible to itraconazole and harbored no cyp51a alterations. the study confirms th ... | 2017 | 28340159 |
| funresdb-a web resource for genotypic susceptibility testing of aspergillus fumigatus. | therapy of invasive aspergillosis is becoming more difficult due to the emergence of azole resistance in aspergillus fumigatus. a majority of resistant strains carries mutations in the cyp51a gene. due to a lack of sensitivity of culture-based methods, molecular detection of a. fumigatus has become an important diagnostic tool. we set up the database funresdb (www.nrz-myk.de/funresdb) to gather all available information about cyp51a-dependent azole resistance from published literature. in summar ... | 2017 | 28340175 |
| synthesis and antimicrobial properties of 4-acylaminobenzenethiosulfoacid s-esters. | a series of esters of 4-acetyl, 4-trifluoroacetyl- and 4-(3-chloropropionyl)aminobenzenethiosulfoacids (twenty-four compounds) were synthesized and characterized by elemental analysis, (1)h nmr and ir spectroscopy. the antibacterial activity of the novel candidates has been screened using the agar diffusion or serial dilution methods against representative gram-positive (staphylococcus aureus, bacillus subtilis, bacillus mesentericus, mycobacterium sp., mycobacterium luteum), gram-negative (aero ... | 2017 | 28344478 |
| differential roles of caspase-1 and caspase-11 in infection and inflammation. | caspase-1, also known as interleukin-1β (il-1β)-converting enzyme (ice), regulates antimicrobial host defense, tissue repair, tumorigenesis, metabolism and membrane biogenesis. on activation within an inflammasome complex, caspase-1 induces pyroptosis and converts pro-il-1β and pro-il-18 into their biologically active forms. "ice(-/-)" or "casp1(-/-)" mice generated using 129 embryonic stem cells carry a 129-associated inactivating passenger mutation on the caspase-11 locus, essentially making t ... | 2017 | 28345580 |
| aspergillus fumigatus native valve infective endocarditis in an otherwise healthy adult. | fungal endocarditis is a rare cause of infective endocarditis, and aspergillus spp. account for up to 30 % of all cases. risk factors include intravenous drug use, immunosuppression, malignancy and the presence of prosthetic valves. | 2016 | 28348749 |
| high cell density cultivation of six fungal strains efficient in azo dye bioremediation. | this work aims at optimizing the high cell density fungal cultivation for producing large quantities of fungal biomass to be used in azo dye residues bioremediation. in our previous studies the efficacy of using certain fungal strains to decolorize a range of commercial textile dyes of different structures (azo, disazo) were investigated. several promising fungal strains belonging to aspergillus tubigenesis, aspergillus niger, aspergillus terreus, and aspergillus fumigates demonstrated high capa ... | 2016 | 28352548 |
| changes in the epidemiological landscape of invasive mould infections and disease. | although a wide variety of pathogens are associated with invasive mould diseases, aspergillus spp. have historically been one of the most common causative organisms. most invasive mould infections are caused by members of the aspergillus fumigatus species complex and an emerging issue is the occurrence of azole resistance in a. fumigatus, with resistance to amphotericin b documented in other aspergillus spp. the epidemiology of invasive fungal disease has shifted in recent years as non-a. fumiga ... | 2017 | 28355462 |
| towards understanding the gliotoxin detoxification mechanism: in vivo thiomethylation protects yeast from gliotoxin cytotoxicity. | gliotoxin (gt) is a mycotoxin produced by some species of ascomycete fungi including the opportunistic human pathogen aspergillus fumigatus. in order to produce gt the host organism needs to have evolved a self-protection mechanism. gt contains a redox-cycling disulfide bridge that is important in mediating toxicity. recently is has been demonstrated that a. fumigatus possesses a novel thiomethyltransferase protein called gtma that has the ability to thiomethylate gt in vivo, which aids the orga ... | 2016 | 28357342 |
| effect of anaerobiasis or hypoxia on pseudomonas aeruginosa inhibition of aspergillus fumigatus biofilm. | pseudomonas aeruginosa (pa) and aspergillus fumigatus (af) are the major bacterial and fungal pathogens in the airways of cystic fibrosis (cf) patients. this is likely related to their ability to form biofilms. both microbes have been associated with cf disease progression. the interplay between these two pathogens has been studied under aerobic conditions, though accumulating data indicates that much of the cf airway is hypoxic or anaerobic. we studied the microbial interaction in these latter ... | 2017 | 28357473 |
| azole-resistant aspergillus fumigatus harboring tr34/l98h, tr46/y121f/t289a and tr53 mutations related to flower fields in colombia. | resistance to triazoles in aspergillus fumigatus has been reported in azole-naive patients in europe, asia, australia and north america. this resistance has been linked to fungicide-driven mutations in the cyp51a gene and its promoter region. we investigated the presence of environmental azole-resistant a. fumigatus strains related to the use of azole fungicides in colombia. soil samples were collected from flower beds, flower fields and public gardens from the outskirts, suburbs and city centre ... | 2017 | 28358115 |
| apoptotic effect of demethoxyfumitremorgin c from marine fungus aspergillus fumigatus on pc3 human prostate cancer cells. | demethoxyfumitremorgin c, a secondary metabolite of the marine fungus, aspergillus fumigatus, had been reported to demonstrate cytotoxic effect on mouse tsft210 cells. however, no information is available regarding its functional mechanism and the chemo-sensitization effects on different kinds of human cancer cells. we found that treatment of demethoxyfumitremorgin c inhibited the cell viability of pc3 human advanced prostate cancer cells, induced apoptosis as determined by annexin v/propidium i ... | 2017 | 28359723 |
| biocontrol potential of halotolerant bacterial chitinase from high yielding novel bacillus pumilus mcb-7 autochthonous to mangrove ecosystem. | the multifaceted role of chitinase in medicine, agriculture, environmental remediation and various other industries greatly demands the isolation of high yielding chitinase producing microorganisms with improved properties. the current study aimed to investigate the isolation, characterization and biocontrol prospective of chitinase producing bacterial strains autochthonous to the extreme conditions of mangrove ecosystems. among the 51 bacterial isolates screened, bacillus pumilus mcb-7 with hig ... | 2017 | 28364802 |
| a proteomic and ultrastructural characterization of aspergillus fumigatus' conidia adaptation at different culture ages. | the airborne fungus aspergillus fumigatus is one of the most common agents of human fungal infections with a remarkable impact on public health. however, a. fumigatus conidia atmospheric resistance and longevity mechanisms are still unknown. therefore, in this work, the processes underlying conidial adaptation were studied by a time course evaluation of the proteomics and ultrastructural changes of a. fumigatus' conidia at three time-points selected according to relevant changes previously estab ... | 2017 | 28365406 |
| functional investigation of iron-responsive microsomal proteins, including mirc, in aspergillus fumigatus. | the functionality of many microsome-associated proteins which exhibit altered abundance in response to iron limitation in aspergillus fumigatus is unknown. here, we generate and characterize eight gene deletion strains, and of most significance reveal that mirc (afua_2g05730) contributes to the maintenance of intracellular siderophore [ferricrocin (fc)] levels, augments conidiation, confers protection against oxidative stress, exhibits an intracellular localization and contributes to fungal viru ... | 2017 | 28367141 |
| inter-country transfer of triazole-resistant aspergillus fumigatus on plant bulbs. | we investigated whether plants imported to ireland from the netherlands might harbor triazole-resistant aspergillus fumigatus. samples of plant bulbs were positive for triazole-resistant a. fumigatus with cyp51a mutations. we hypothesize that this represents a route for inter-country transfer of an emerging resistance mechanism in a major opportunistic mold pathogen. | 2017 | 28369271 |