Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| listeriosis associated with gorgonzola (italian blue-veined cheese). | we describe a case of listeriosis in italy associated with the consumption of cheese. opened samples of two brands of gorgonzola (italian blue-veined cheese; referred to as brands "b" and "c") were collected from the patient's refrigerator. unopened samples of the brand suspected to be the source of infection (brand b) were taken from the store where the cheese had been purchased, other local stores, and the production plant. listeria monocytogenes serotype 1/2b was isolated from the patient and ... | 2006 | 16761945 |
| characterization of a spontaneous, pressure-tolerant listeria monocytogenes scott a ctsr deletion mutant. | a spontaneous, pressure-tolerant mutant of listeria monocytogenes scott a, designated 2-1, was isolated after several rounds of pressure treatments at 500 mpa for 10 min. mutant 2-1 was almost 100,000-fold more resistant than the wild type to a pressure of 350 mpa, and about 100-fold more resistant to 450 mpa when pressurized in growth medium. approximately ten times more mutant cells than wild-type cells survived a 20-min exposure to 55 degrees c, and the mutant appears also to be more resistan ... | 2006 | 16761946 |
| simultaneous assessment of antigen-stimulated cytokine production and memory subset composition of memory cd8 t cells. | the functional identification of antigen-specific cd8 t cell populations is critical to understanding host responses to infection by intracellular pathogens. furthermore, assessing the properties of protective memory cd8 t cell populations generated by immunization is necessary in the rational design of vaccines. recently, a classification scheme was proposed in which memory cd8 t cells were divided into one of two distinct subsets, based on cd62l expression, that have different functional prope ... | 2006 | 16762359 |
| effect of the inoculum size on listeria monocytogenes growth in structured media. | obtaining quantitative data concerning the relative impact of various factors that may influence bacterial growth is of great importance for microbial risk assessment and predictive microbiology. the objective of this work was to investigate the effect of the initial listeria monocytogenes density on all the growth parameters of this pathogen (lag phase duration, growth rate and maximum population density attained) on a sterile solid model system mimicking smoked fishery products, and in real co ... | 2006 | 16762438 |
| low virulence but potentially fatal outcome-listeria ivanovii. | of the six known species of listeria, listeria monocytogenes is classified as the only human pathogen causing listeriosis, a highly fatal, opportunistic infection contracted via food. listeria ivanovii is the only other pathogenic species of the genus and is considered to be specific to ruminants, except for extremely rare cases of infection in humans. we report here on a 64-year-old male patient with l. ivanovii who responded favorably to treatment with intravenous ampicillin. | 2006 | 16762780 |
| cholestasis protects the liver from ischaemic injury and post-ischaemic inflammation in the mouse. | cholestasis is associated with high morbidity and mortality in patients undergoing major liver surgery, but the mechanisms responsible remain elusive. increased ischaemic liver injury and inflammation may contribute to the poor outcome. | 2007 | 16763052 |
| characterization of listeria monocytogenes protein lmo0327 with murein hydrolase activity. | listeria monocytogenes is an ubiquitous gram-positive, opportunistic food-borne human and animal pathogen. to date, five l. monocytogenes autolysins have been characterized: p60, p45, ami, mura and auto and the preliminary results of our studies show that flaa, a flagellar protein of l. monocytogenes, also has murein-degrading activity. in this study, a gene coding a 144 kda protein (lmo0327) with murein hydrolase activity was identified from a lambda zap expression library of l. monocytogenes e ... | 2006 | 16763838 |
| [antibiotic susceptibility of listeria monocytogenes strains isolated in the primor'e region]. | antibiotic susceptibility of the listeria monocytogenes isolates from biotic and abiotic objects of the environment in the primor'e region was estimated. 100% of the isolates proved to be susceptible to benzylpenicillin, ampicillin, carbenicillin, gentamicin, doxycycline, tetracycline, vancomycin, cefazolin and rifampicin. 96, 92 and 84% of the isolates were susceptible to roxithromycin, clarithromycin and ofloxacin respectively. no significant differences were detected in the susceptibility of ... | 2005 | 16768211 |
| short communication: effect of exopolysaccharide isolated from "viili" on the adhesion of probiotics and pathogens to intestinal mucus. | the strong ropy character of the scandinavian fermented milk viili is conferred by the exopolysaccharides (eps) produced by lactococcal strains. these biopolymers can be responsible for some health benefits. we have assessed the influence of the eps fraction isolated from commercial viili on the adhesion of some probiotics and pathogens to human intestinal mucus. concentrations of viili eps greater than 0.1 mg/ml promoted a decrease in adherence of bifidobacterium lactis bb12 and lactobacillus r ... | 2006 | 16772550 |
| a survey of foodborne pathogens in bulk tank milk and raw milk consumption among farm families in pennsylvania. | a 2-part study was conducted to determine the risk of exposure to human pathogens from raw milk. the first part of the study focused on determining raw milk consumption habits of dairy producers. a total of 248 dairy producers from 16 counties in pennsylvania were surveyed. overall, 105 (42.3%) of the 248 dairy producers consumed raw milk and 170 (68.5%) of the 248 dairy producers were aware of foodborne pathogens in raw milk. dairy producers who were not aware of foodborne pathogens in raw milk ... | 2006 | 16772561 |
| invasive listeria monocytogenes infections in the netherlands, 1995-2003. | in order to add to the limited data available about the incidence of invasive listeria monocytogenes infection in the netherlands, two studies were conducted. in the first study, data on hospital patients with listeriosis in the period 1995-2003 were obtained from the national medical registration (study 1). in the second study, hospital discharge letters for patients whose listeria isolates were received by the netherlands reference laboratory for bacterial meningitis (nrlbm) in the period 1999 ... | 2006 | 16773392 |
| listeriolysin o: a key protein of listeria monocytogenes with multiple functions. | cholesterol-dependent cytolysins (cdcs) are produced by a large number of pathogenic gram-positive bacteria. most of these single-chain proteins are secreted in the extracellular medium. among the species producing cdcs, only two species belonging to the genus listeria (listeria monocytogenes and listeria ivanovii) are able to multiply intracellularly and release their toxins in the phagosomal compartment of the infected host cell. this review provides an updated overview on the importance of li ... | 2006 | 16774585 |
| purification and characterization of a heat-stable serine protease inhibitor from the tubers of new potato variety "golden valley". | potide-g, a small (5578.9 da) antimicrobial peptide, was isolated from potato tubers (solanum tuberosum l. cv. golden valley) through extraction of the water-soluble fraction, dialysis, ultrafiltration and deae-cellulose and c18 reverse-phase high performance liquid chromatography. this antimicrobial peptide was heat-stable and almost completely suppressed the proteolytic activity of trypsin, chymotrypsin and papain, with no hemolytic activity. in addition, potide-g potently inhibited growth of ... | 2006 | 16777063 |
| biofilm formation in an ice cream plant. | the sites of biofilm formation in an ice cream plant were investigated by sampling both the production line and the environment. experiments were carried out twice within a 20-day period. first, stainless steel coupons were fixed to surfaces adjacent to food contact surfaces, the floor drains and the doormat. they were taken for the analysis of biofilm at three different production stages. then, biofilm forming bacteria were enumerated and also presence of listeria monocytogenes was monitored. b ... | 2006 | 16779628 |
| characterization of a new bacteriocin produced from a novel isolated strain of bacillus lentus ng121. | the new bacteriocin is produced from bacillus lentus ng121 isolated from khameera - a traditional fermented food from himachal pradesh, india which has been reported for the first time in the literature to produce bacteriocin and exhibited very high activity units of 20 x 10(5) au (arbitrary units)/ml. this bacteriocin was partially purified and was further characterized to assess its preservation characteristics. it showed strong antimicrobial activity against the most challenging and serious t ... | 2006 | 16779629 |
| [ruptured mesenteric aneurysm from listeria monocytogenes]. | infections due to listeria monocytogenes usually occur in pregnant women, in elderly or immunocompromised patients. we report the case of an immunosuppressed woman who had been hospitalized for a meningoencephalitis due to l. monocytogenes. she was operated on for an abdominal hemorrhagic shock due to a ruptured mycotic aneurysm of the inferior mesenteric artery. arterial aneurysms due to this germ are rare and occur on large arteries. only 17 cases have been previously described in the literatu ... | 2006 | 16780785 |
| adsorption on stainless steel surfaces of biosurfactants produced by gram-negative and gram-positive bacteria: consequence on the bioadhesive behavior of listeria monocytogenes. | the ability of adsorbed biosurfactants (pf and lb) obtained from gram-negative bacterium (pseudomonas fluorescens) or gram-positive bacterium (lactobacillus helveticus) to inhibit adhesion of four listerial strains to stainless steel was investigated. these metallic surfaces were characterized using the following complementary analytical techniques: contact-angle measurements (cam), atomic force microscopy (afm), polarization modulation-infrared reflection-adsorption spectroscopy (pm-irras) and ... | 2006 | 16781848 |
| ultrasound markers of fetal infection, part 2: bacterial, parasitic, and fungal infections. | up to 1% of all pregnancies have clinically overt intra-amniotic bacterial infections, and an even larger percentage of pregnant women may be affected by silent infections. although most pregnant women with overt intra-amniotic bacterial infection have experienced prolonged rupture of membranes (prom), symptomatic and most silent nonviral intra-amniotic infections may occur with intact membranes. the etiology of intra-amniotic infection after prom is almost always polymicrobial and consists of g ... | 2006 | 16783243 |
| acute symptomatic hydrocephalus in listeria monocytogenes meningitis. | listeria monocytogenes meningitis appears to have increased in incidence. although most reported cases of listeriosis involve the central nervous system, brain computed tomography is usually normal. hydrocephalus is a common complication of tuberculous meningitis, which has a high prevalence in taiwan. however, patients with l. monocytogenes meningitis rarely develop the complication of symptomatic hydrocephalus. we report a patient with l. monocytogenes meningitis who presented with persistent ... | 2006 | 16783458 |
| evidence for the opposing roles of different gamma delta t cell subsets in macrophage homeostasis. | to ensure invading pathogens are eliminated with minimal damage to host tissues it is essential that macrophage activation be tightly regulated. previously we demonstrated that a subset of gammadelta t cells (vgamma1(+)) contributes to resolving pathogen-induced immune responses by killing activated macrophages. however, the exaggerated macrophage response seen in infected vgamma1(+) t cell-deficient mice suggests that gammadelta t cells play a broader role in macrophage homeostasis and other su ... | 2006 | 16783854 |
| a stochastic analysis of a brownian ratchet model for actin-based motility. | in recent single-particle tracking (spt) measurements on listeria monocytogenes motility in cells [kuo and mcgrath (2000)], the actin-based stochastic dynamics of the bacterium movement has been analyzed statistically in terms of the mean-square displacement (msd) of the trajectory. we present a stochastic analysis of a simplified polymerization brownian ratchet (br) model in which motions are limited by the bacterium movement. analytical results are obtained and statistical data analyses are in ... | 2004 | 16783923 |
| recovery of heat-injured listeria innocua. | listeria innocua was subjected to thermal inactivation and the extent of heat-injured cells was quantified. cultures were heated in liquid medium for different times, using temperatures in the range of 52.5 to 65.0 degrees c, and plated on tryptic soy agar with 0.6% yeast extract (tsaye) used as non-selective medium and on tsaye plus 5% nacl (tsaye+nacl) and palcam agar with selective supplement (palcam agar) as selective media. the difference observed in counts in non-selective and in selective ... | 2006 | 16784792 |
| self-antigen maintains the innate antibacterial function of self-specific cd8 t cells in vivo. | self-specific cd8 t cells, which are selected by high-affinity interactions with self-ags, develop into a lineage distinct from conventional cd8 t cells. we have previously shown that these self-specific cells acquire phenotypic and functional similarities to cells of the innate immune system including the expression of functional receptors associated with nk cells. in this study, we show that these self-specific cells have the ability to produce large amounts of ifn-gamma in response to infecti ... | 2006 | 16785508 |
| division of labor and cooperation between dendritic cells. | 2006 | 16785884 | |
| a rapid molecular-based assay for direct quantification of viable bacteria in slaughterhouses. | a rapid test for microbial quantification in carcass and environmental swabs that does not require enrichment and provides results in less than 4 h is described here. steps in the assay include the rapid concentration of bacteria on sponge swabs by vacuum filtration followed by real-time pcr detection. the assay has been applied for the detection of coliforms, escherichia coli o157:h7, salmonella spp., and listeria monocytogenes on carcass swabs and environmental samples in a slaughterhouse-proc ... | 2006 | 16786844 |
| chlorine resistance of listeria monocytogenes biofilms and relationship to subtype, cell density, and planktonic cell chlorine resistance. | strains of listeria monocytogenes vary in their ability to produce biofilms. this research determined if cell density, planktonic chlorine resistance, or subtype are associated with the resistance of l. monocytogenes biofilms to chlorine. thirteen strains of l. monocytogenes were selected for this research based on biofilm accumulation on stainless steel and rep-pcr subtyping. these strains were challenged with chlorine to determine the resistance of individual strains of l. monocytogenes. plank ... | 2006 | 16786848 |
| characterization of listeria monocytogenes isolates from 50 small-scale austrian cheese factories. | one hundred eighty-one small-scale cheese factories (annual production < 100,000 kg) were tested for the presence of listeria monocytogenes in cheese and smear samples from 1997 to 2000. in total, 2615 samples were drawn. fifty (27.6%) of 181 enterprises yielded l. monocytogenes. from 14 of the cheese-making facilities, we obtained more than four l. monocytogenes isolates. a total of 182 mostly cheese- and smear-borne l. monocytogenes strains were characterized by serotyping and pulsed-field gel ... | 2006 | 16786849 |
| contamination of cooked peeled shrimp (pandalus borealis) by listeria monocytogenes during processing at two processing plants. | listeria spp. and listeria monocytogenes contamination was evaluated in cooked peeled shrimp (final or semifinal product, 82 samples) and the shrimp-processing environment (two plants, 613 samples) in eight surveys conducted from 1998 through 2001. listeria was detected in 12.5% (78) of the 695 samples (11.2% of the samples were positive for l. monocytogenes), but none of the samples of final product contained listeria. one hundred seventy-two l. monocytogenes isolates were characterized by puls ... | 2006 | 16786850 |
| determination of the growth limits and kinetic behavior of listeria monocytogenes in a sliced cooked cured meat product: validation of the predictive growth model under constant and dynamic temperature storage conditions. | to describe the growth limits of listeria monocytogenes nctc10527 in a sliced vacuum-packaged cooked cured meat product, the binary logistic regression model was used to develop an equation to determine the probability of growth or no growth of l. monocytogenes as a function of temperature (from 0 to 10 degrees c) and water activity (from 0.88 to 0.98). two inoculum concentrations were used (10 and 10(4) cfu g(-1)), and the growth limits for the two inocula were different. the kinetic behavior o ... | 2006 | 16786851 |
| inhibition of listeria innocua in hummus by a combination of nisin and citric acid. | the effect of nisin or citric acid or combinations of these two inhibitors on the inactivation of a cocktail of three listeria innocua strains was investigated in a model brain heart infusion (bhi) broth and hummus (chickpea dip). in bhi broth, citric acid had a limited ability to inhibit l. innocua growth. nisin initially reduced l. innocua concentrations by about 3 log cycles; however, l. innocua reached concentrations similar to those of the control after 5 days at 22 degrees c. in combinatio ... | 2006 | 16786852 |
| effect of cheese water activity and carbohydrate content on the barotolerance of listeria monocytogenes scott a. | high-pressure processing is an appropriate technique for improving the microbiological safety of packaged ready-to-eat foods. the effect of high-pressure treatment on listeria monocytogenes scott a inoculated into fresh hispánico-type cheese and ripe mahón cheese was investigated. a 3.8-log reduction in the counts of l. monocytogenes scott a in fresh cheese was recorded after 3 min at 400 mpa and 12 degrees c, whereas 18 min under the same conditions was required to obtain a 1-log reduction in r ... | 2006 | 16786853 |
| deoxynivalenol and satratoxin g potentiate proinflammatory cytokine and macrophage inhibitory protein 2 induction by listeria and salmonella in the macrophage. | health risks from microbial pathogens and toxins encountered in food and the environment continue to be of worldwide concern. the purpose of this research was to test the hypothesis that trichothecene mycotoxins amplify inflammatory responses to foodborne bacterial pathogens. we assessed the capacity of deoxynivalenol (don) and satratoxin g (sg) to potentiate chemokine and proinflammatory cytokine production in raw 264.7 murine macrophages induced by listeria monocytogenes and salmonella typhimu ... | 2006 | 16786854 |
| pulsed-field gel electrophoresis typing of listeria monocytogenes isolated in two finnish fish farms. | the aim of this study was to find sources of listeria monocytogenes contamination in fish products from a fish farm. the occurrence of l. monocytogenes also was compared in two freshwater fish farms with different types of fishponds. samples collected from chilled rainbow trout (oncorhynchus mykiss) and the slaughterhouse environment did not contain l. monocytogenes, but listeria innocua was found in two samples from the slaughterhouses. ten isolates of l. monocytogenes were discovered in sedime ... | 2006 | 16786871 |
| cold stress tolerance of listeria monocytogenes: a review of molecular adaptive mechanisms and food safety implications. | the foodborne pathogen listeria monocytogenes has many physiological adaptations that enable survival under a wide range of environmental conditions. the microbes overcome various types of stress, including the cold stress associated with low temperatures in food-production and storage environments. cold stress adaptation mechanisms are therefore an important attribute of l. monocytogenes, enabling these food pathogens to survive and proliferate to reach minimal infectious levels on refrigerated ... | 2006 | 16786878 |
| performance evaluation of a low conductive growth medium (lcgm) for growth of healthy and stressed listeria monocytogenes and other common bacterial species. | the performance of a low conductive growth medium (lcgm) (conductivity of <1300 micros) was evaluated for its ability to support growth of food borne bacterial pathogens including listeria monocytogenes and to determine the expression of the two key virulence proteins in l. monocytogenes for possible applications in an impedance-based microfluidic biochip detection platform. growth of listeria was monitored spectrophotometrically and the lag phase, generation time, growth rate and maximum popula ... | 2006 | 16790285 |
| comparison of different live vaccine strategies in vivo for delivery of protein antigen or antigen-encoding dna and mrna by virulence-attenuated listeria monocytogenes. | listeria monocytogenes can be used to deliver protein antigens or dna and mrna encoding such antigens directly into the cytosol of host cells because of its intracellular lifestyle. in this study, we compare the in vivo efficiencies of activation of antigen-specific cd8 and cd4 t cells when the antigen is secreted by l. monocytogenes or when antigen-encoding plasmid dna or mrna is released by self-destructing strains of l. monocytogenes. infection of mice with self-destructing l. monocytogenes c ... | 2006 | 16790768 |
| surface plasmon resonance biosensors for detection of pathogenic microorganisms: strategies to secure food and environmental safety. | this review describes the exploitation of exclusively optical surface plasmon resonance (spr) biosensors for the direct and indirect detection of pathogenic microorganisms in food chains and the environment. direct detection is, in most cases, facilitated by the use of defined monoclonal or polyclonal antibodies raised against (a part of) the target pathogenic microorganisms. the antibodies were immobilized to a solid phase of the sensor to capture the microbe from the sample. alternatively, ant ... | 2006 | 16792081 |
| biosensors for the analysis of food- and waterborne pathogens and their toxins. | biosensors are devices which combine a biochemical recognition element with a physical transducer. there are various types of biosensors, including electrochemical, acoustical, and optical sensors. biosensors are used for medical applications and for environmental testing. although biosensors are not commonly used for food microbial analysis, they have great potential for the detection of microbial pathogens and their toxins in food. they enable fast or real-time detection, portability, and mult ... | 2006 | 16792089 |
| recovery and characterization of a 30.7-kda protein from bacillus licheniformis associated with inhibitory activity against methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, and listeria monocytogenes. | of 131 bacterial isolates from seaweed, a culture of bacillus licheniformis produced a novel protein with antibacterial activity against methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, and listeria monocytogenes. the antibacterial activity was maximal in cultures prepared in columbia broth containing pieces of synthetic polyurethane sponge and shaken at 210 to 230 rpm. antibacterial activity was not found in cultures grown statically or with different speeds of rot ... | 2006 | 16794953 |
| bioassay-guided purification and identification of antimicrobial components in chinese green tea extract. | the chinese green tea extract was found to strongly inhibit the growth of major food-borne pathogens, escherichia coli o157:h7, salmonella typhimurium dt104, listeria monocytogenes, staphylococcus aureus, and a diarrhoea food-poisoning pathogen bacillus cereus, by 44-100% with the highest activity found against s. aureus and lowest against e. coli o157:h7. a bioassay-guided fractionation technique was used for identifying the principal active component. a simple and efficient reversed-phase high ... | 2006 | 16797571 |
| simultaneous detection by pcr of escherichia coli, listeria monocytogenes and salmonella typhimurium in artificially inoculated wheat grain. | a multiplex pcr procedure was established to detect escherichia coli, listeria monocytogenes and salmonella typhimurium in artificially inoculated wheat grain. the pcr protocol with an enrichment step successfully detected all three organisms inoculated together in non-autoclaved wheat grain. after a one day enrichment, e. coli, l. monocytogenes and s. typhimurium were detected at levels of 56, 1800 and <54 cfu/ml, respectively, in the initial sample. for l. monocytogenes, an improved detection ... | 2006 | 16797761 |
| identification of a cross-reactive hla-drb1*0301-restricted cd4 t cell response directed against cholesterol-binding cytolysins from two different pathogens. | cholesterol-binding cytolysins constitute an evolutionarily conserved family of pore-forming proteins expressed by different gram-positive pathogens. listeriolysin o, one well-characterized member of the cytolysin family, is also known to induce specific cd4 and cd8 t cell responses upon infection of mice with listeria monocytogenes. here we describe an hla-drb1*0301-restricted listeriolysin o-derived t cell epitope that is conserved among several members of the cytolysin family. an hla-drb1*030 ... | 2006 | 16798043 |
| outbreak of human listeriosis associated with tomme cheese in northwest switzerland, 2005. | during an eight week period in spring 2005, 10 cases of listeriosis were reported in a small area of northwest switzerland (150,000 inhabitants). eight cases were in older immunocompromised patients who became ill with bacteraemia (three deaths), and two cases were in pregnant women who had septic abortion. all cases were due to a serotype 1/2a isolate with one of two pulsovars found by pfge. patient interviews quickly revealed that a locally made and distributed soft cheese (known as 'tomme') w ... | 2006 | 16801693 |
| listeria outbreak associated with sandwich consumption from a hospital retail shop, united kingdom. | an outbreak of listeriosis occurred in the swindon area of the uk in autumn 2003. five cases were detected in pregnant women. four of these women were thought to have eaten prepacked sandwiches from a retail outlet in one particular hospital. sampling at the supplier detected listeria monocytogenes, which was indistinguishable on molecular testing from the patients isolates. recent changes in uk food legislation should help diminish the risk of further outbreaks/cases such as ours occurring. | 2006 | 16801694 |
| significant increase of listeriosis in germany--epidemiological patterns 2001-2005. | listeriosis has been a mandatorily notifiable disease in germany since january 2001. clinical cases with isolation of listeria monocytogenes from sterile specimens or neonates are reported to the robert koch-institut. listeriosis incidence significantly increased from 0.26 per 100 000 inhabitants (217 cases) in 2001 to 0.62 per 100 000 (519 cases) in 2005. the increase only occurred among non pregnancy-associated cases and was mainly due to a rise in cases in the age group > or =60 years. the hi ... | 2006 | 16801695 |
| surveillance of listeriosis in finland during 1995-2004. | we analysed the surveillance data from listeriosis cases notified to the finnish national infectious diseases register between 1995 and 2004 and describe our recent experience in investigating clusters of listeriosis cases. the number of annual cases varied between 18 and 53 but no trends in incidence were identified (average annual incidence was 7 cases per million inhabitants). only a few cases affected pregnant women or newborns. most of the patients were elderly people with non-malignant und ... | 2006 | 16801696 |
| surveillance of human listeriosis in france, 2001-2003. | mandatory notification of listeriosis began in france in 1999. enhanced public health surveillance, including routine molecular characterisation of listeria monocytogenes strains, epidemiologic follow up of cases, and collection of food samples, has improved the sensitivity of outbreak detection and response. the incidence of listeriosis declined from 4.5 cases/million in 1999-2000 to approximately 3.5 cases/million during the period 2001-2003. clinical, demographic and microbiological character ... | 2006 | 16801697 |
| a multifunctional micro-fluidic system for dielectrophoretic concentration coupled with immuno-capture of low numbers of listeria monocytogenes. | in this study, we demonstrated a micro-fluidic system with multiple functions, including concentration of bacteria using dielectrophoresis (dep) and selective capture using antibody recognition, resulting in a high capture efficiency of bacterial cells. the device consisted of an array of oxide covered interdigitated electrodes on a flat silicon substrate and a approximately 16 microm high and approximately 260 microm wide micro-channel within a pdms cover. for selective capture of listeria mono ... | 2006 | 16804594 |
| fluorescence microscopy of nacl-stressed, elongated salmonella and listeria cells reveals the presence of septa in filaments. | the cell morphology of salmonella enteritidis and listeria monocytogenes after the application of stress was examined. cells were stressed by plating the bacteria on tryptone soya agar (tsa) plates, with 5-10% nacl. the plates were subsequently incubated for 6 days at 25 degrees c. finally, the cells were harvested and subjected to different fluorescent probes in order to visualize the possible presence of septa in elongated cells. use of the stain 4',6-diamidino-2-phenylindole (dapi), which is ... | 2006 | 16806551 |
| first results of the active surveillance of listeria monocytogenes infections in the netherlands reveal higher than expected incidence. | 2006 | 16809829 | |
| structure-based synthetic mimicry of discontinuous protein binding sites: inhibitors of the interaction of mena evh1 domain with proline-rich ligands. | the mena evh1 domain, a protein-interaction module involved in actin-based cell motility, recognizes proline-rich ligand motifs, which are also present in the sequence of the surface protein acta of listeria monocytogenes. the interaction of acta with host mena evh1 enables the bacterium to actively recruit host actin in order to spread into neighboring cells. based on the crystal structure of mena evh1 in complex with a polyproline peptide ligand, we have generated a range of assembled peptides ... | 2006 | 16810654 |
| technological characterization of a bacteriocin-producing lactobacillus sakei and its use in fermented sausages production. | the aim of this paper was the technological characterization of a lactobacillus sakei strain, able to produce the bacteriocin sakacin p, that was originally isolated from naturally fermented sausages. experiments were conducted in situ, using mrs-based medium, and in situ, when the strain was inoculated as starter culture in real sausage fermentation. the results obtained underlined that the strain was able to grow in conditions that are commonly used in the production line, and only lactose and ... | 2006 | 16814893 |
| detection of listeria monocytogenes in food using a combined enrichment/real-time pcr method targeting the prfa gene. | a combined enrichment/real-time pcr method for the detection of listeria monocytogenes is presented. the method is based on a conventional pcr assay targeting the prfa gene, which has been validated and suggested as an international standard pcr method for identifying l. monocytogenes in food. this real-time pcr assay includes an internal amplification control. inclusivity and exclusivity were 100% each when testing 100 l. monocytogenes isolates, 30 listeria spp. isolates other than l. monocytog ... | 2006 | 16814987 |
| pathogenic role for virus-specific cd4 t cells in mice with coronavirus-induced acute encephalitis. | acute viral encephalitis is believed to result from direct virus destruction of infected cells and from virus-induced host immune response, but the relative contribution of each remains largely unknown. for example, c57bl/6 (b6) mice infected with mouse hepatitis virus (jhm strain, jhmv) develop severe encephalitis, with death occurring within 7 days. here, we show that the host response to a single jhmv-specific immunodominant cd4 t-cell epitope is critical for severe disease. we engineered a r ... | 2006 | 16816374 |
| comparison of the concentrations of phenolic compounds in olive oils and other plant oils: correlation with antimicrobial activity. | the antimicrobial activity of different edible vegetable oils was studied. in vitro results revealed that the oils from olive fruits had a strong bactericidal action against a broad spectrum of microorganisms, this effect being higher in general against gram-positive than gram-negative bacteria. thus, olive oils showed bactericidal activity not only against harmful bacteria of the intestinal microbiota (clostridium perfringens and escherichia coli) also against beneficial microorganisms such as ... | 2006 | 16819902 |
| differential expansion, activation and effector functions of conventional and plasmacytoid dendritic cells in mouse tissues transiently infected with listeria monocytogenes. | dendritic cells (dc) are crucial in generating immunity to infection. here we characterize changes in dc in terms of number, activation and effector functions, focusing on conventional dc (cdc) and plasmacytoid dc (pdc), in listeria-infected mice. kinetic studies showed a subset- and tissue-specific expansion of cdc and upregulation of cd80 and cd86 on splenic and mesenteric lymph node (mln) cdc after intragastric infection. expansion of pdc was more prolonged than cdc, and pdc upregulated cd86 ... | 2006 | 16819969 |
| isolation and characterization of listeria monocytogenes isolates from ready-to-eat foods in florida. | of 3,063 ready-to-eat food samples tested, 91 (2.97%) were positive for listeria monocytogenes, and lineage 1 strains outnumbered lineage 2 strains 57 to 34. seventy-one isolates (78%) exhibited multiple antibiotic resistance, and an l. monocytogenes-specific bacteriophage cocktail lysed 65 of 91 (71%) isolates. determining phage, acid, and antibiotic susceptibility phenotypes enabled us to identify differences among strains which were otherwise indistinguishable by conventional methods. | 2006 | 16820508 |
| feature extraction from light-scatter patterns of listeria colonies for identification and classification. | bacterial contamination by listeria monocytogenes not only puts the public at risk, but also is costly for the food-processing industry. traditional biochemical methods for pathogen identification require complicated sample preparation for reliable results. optical scattering technology has been used for identification of bacterial cells in suspension, but with only limited success. therefore, to improve the efficacy of the identification process using our novel imaging approach, we analyze bact ... | 2006 | 16822056 |
| inhibition of membrane bound atpases of escherichia coli and listeria monocytogenes by plant oil aromatics. | previous studies have reported that the mechanism of bactericidal action of the plant oil aromatics, eugenol, carvacrol and cinnamaldehyde involves inhibition of adenosine triphosphate generation and membrane disruption. in this study the capacity of the aromatics to inhibit the membrane bound atpase activity of escherichia coli and listeria monocytogenes was investigated by experiments on isolated membranes. inhibition of the atpase activity of e. coli membranes was observed with 5 mm or 10 mm ... | 2006 | 16828188 |
| phage for rapid detection and control of bacterial pathogens in food. | 2006 | 16829259 | |
| an updated review of listeria monocytogenes in the pork meat industry and its products. | pork meat and processed pork products have been the sources of outbreaks of listeriosis in france and in other european countries during the last decade. the aim of this review is to understand how contamination, survival and growth of listeria monocytogenes can occur in pork meat products. this study discusses the presence of l. monocytogenes in raw pork meat, in the processing environment and in finished products. the prevalence of l. monocytogenes generally increases from the farm to the manu ... | 2006 | 16834586 |
| comparative evaluation of adhesion, surface properties, and surface protein composition of listeria monocytogenes strains after cultivation at constant ph of 5 and 7. | to analyse the cellular mechanisms that influence listeria monocytogenes adhesion onto inert surfaces under acidic growth conditions. | 2006 | 16834591 |
| antimicrobial potential of four lactobacillus strains isolated from breast milk. | the antimicrobial potential of four lactobacilli (lactobacillus salivarius cect5713, lactobacillus gasseri cect5714, l. gasseri cect5715 and lactobacillus fermentum cect5716), isolated from fresh human breast milk, was evaluated in this study and compared with lactobacillus coryniformis cect5711, a reuterin-producing strain isolated from an artisan goat's cheese. | 2006 | 16834593 |
| the cadmium transport sites of cada, the cd2+-atpase from listeria monocytogenes. | cada, the cd(2+)-atpase from listeria monocytogenes, belongs to the zn(2+)/cd(2+)/pb(2+)-atpase bacterial subfamily of p(1b)-atpases that ensure detoxification of the bacteria. whereas it is the major determinant of listeria resistance to cd(2+), cada expressed in saccharomyces cerevisiae severely decreases yeast tolerance to cd(2+) (wu, c. c., bal, n., pérard, j., lowe, j., boscheron, c., mintz, e., and catty, p. (2004) biochem. biophys. res. commun. 324, 1034-1040). this phenotype, which refle ... | 2006 | 16835223 |
| characterization of a bacteriocin-like substance produced by bacillus amyloliquefaciens isolated from the brazilian atlantic forest. | a bacillus strain producing a bacteriocin-like substance was characterized by biochemical profiling and 16s rdna sequencing. phylogenetic analysis indicated that the strain has high sequence similarity with bacillus amyloliquefaciens. the antimicrobial substance was inhibitory to pathogenic and food-spoilage bacteria, such as listeria monocytogenes, bacillus cereus, serratia marcescens, and pasteurella haemolytica. it was stable over a wide temperature range, but lost activity when the temperatu ... | 2006 | 16835841 |
| crystal structure of phosphatidylglycerophosphatase (pgpase), a putative membrane-bound lipid phosphatase, reveals a novel binuclear metal binding site and two "proton wires". | phosphatidylglycerophosphatase (pgpase), an enzyme involved in lipid metabolism, catalyzes formation of phosphatidylglycerol from phosphatidylglycerophosphate. phosphatidylglycerol is a multifunctional phospholipid, found in the biological membranes of many organisms. here, we report the crystal structure of listeria monocytogenes pgpase at 1.8 a resolution. pgpase, an all-helical molecule, forms a homotetramer. each protomer contains an independent active site with two metal ions, ca(2+) and mg ... | 2006 | 16838328 |
| ecology of listeria spp. in a fish farm and molecular typing of listeria monocytogenes from fish farming and processing companies. | this study focused on the ecology of listeria monocytogenes in a fish farm by following the changes in its occurrence in different types of samples for a three year period. in addition, l. monocytogenes isolates from different seafood industry areas were compared with pulsed field gel electrophoresis (pfge) typing to discover possible associations between primary production, further processing and final products. weather conditions were found to have a strong influence on the probability of find ... | 2006 | 16842875 |
| serological and molecular ecology of listeria monocytogenes isolates collected from 13 french pork meat salting-curing plants and their products. | the purpose of this study was dual: 1. to evaluate the serotype distribution of 1028 listeria monocytogenes isolates collected in 13 french salting factories and their products and 2. to identify sources of l. monocytogenes contamination in these factories and trace the routes of spread by pfge (pulsed-field gel electrophoresis) typing. serotypes 1/2a, 1/2b, 1/2c, 4b and 4e occurred. pulsotype diversity was high among strains collected in plants and products. furthermore, strains showing similar ... | 2006 | 16843563 |
| isolation of bacteriocinogenic lactobacillus plantarum strains from ben saalga, a traditional fermented gruel from burkina faso. | a collection of lactic acid bacteria isolated from ben saalga, a traditional fermented gruel from burkina faso, was screened for bacteriocin production. seven isolates were selected for their broad antimicrobial spectra, which overall included strains of bacillus cereus, bacillus licheniformis, enterococcus faecalis, listeria innocua, listeria monocytogenes, staphylococcus aureus, escherichia coli and salmonella enterica. cluster analysis of rapd-pcr patterns revealed that six of the isolates re ... | 2006 | 16844251 |
| ph and solute concentration of suspension media affect the outcome of high hydrostatic pressure treatment of listeria monocytogenes. | the effect of ph and solute concentration of suspension media on high hydrostatic pressure (hhp) induced inactivation of listeria monocytogenes (approximate 10(8) cfu/ml) was investigated by the using treatment between 300 mpa and 600 mpa at 25 degrees c for 10 min. the suspension media used in this study represented different concentrations (0.1% to 10%) of buffered peptone water (bpw) with an adjusted ph of 4 to 7. an increase in the concentration of bpw resulted in a decreased hhp-induced ina ... | 2006 | 16844252 |
| listeria monocytogenes traffics from maternal organs to the placenta and back. | infection with listeria monocytogenes is a significant health problem during pregnancy. this study evaluates the role of trafficking between maternal organs and placenta in a pregnant guinea pig model of listeriosis. after intravenous inoculation of guinea pigs, the initial ratio of bacteria in maternal organs to placenta was 10(3)-10(4):1. rapid increase of bacteria in the placenta changed the ratio to 1:1 after 24 h. utilizing two wild-type strains, differentially marked by their susceptibilit ... | 2006 | 16846254 |
| disposable electrochemical genosensor for the simultaneous analysis of different bacterial food contaminants. | this paper deals with the use of an electrochemical genosensor array for the rapid and simultaneous detection of different food-contaminating pathogenic bacteria. the method includes pcr amplification followed by analysis of the amplicons by hybridisation with toxin-specific oligonucleotide probes. a screen-printed array of four gold electrodes, modified using thiol-tethered oligonucleotide probes, was used. unmodified pcr products were captured at the sensor interface via sandwich hybridisation ... | 2007 | 16846731 |
| delayed expansion and contraction of cd8+ t cell response during infection with virulent salmonella typhimurium. | ag presentation to cd8(+) t cells often commences immediately after infection, which facilitates their rapid expansion and control of infection. subsequently, the primed cells undergo rapid contraction. we report that this paradigm is not followed during infection with virulent salmonella enterica, serovar typhimurium (st), an intracellular bacterium that replicates within phagosomes of infected cells. although susceptible mice die rapidly (approximately 7 days), resistant mice (129 x 1svj) harb ... | 2006 | 16849458 |
| visualization of il-12/23p40 in vivo reveals immunostimulatory dendritic cell migrants that promote th1 differentiation. | il-12p40 is induced in macrophages and dendritic cells (dc) after activation by microbial tlr ligands and cytokines and constitutes a component of il-12 and il-23. in an effort to understand the location and kinetics of these cytokines during the course of an immune response, we generated knockin (gene-targeted) mice that express the p40 gene linked via a viral internal ribosome entry site element with fluorescent reporters, eyfp or egfp. macrophages and dc from these mice faithfully reported bi ... | 2006 | 16849470 |
| innate inflammatory signals induced by various pathogens differentially dictate the ifn-i dependence of cd8 t cells for clonal expansion and memory formation. | type-i ifns (ifn-i) provide direct survival signals to t cells during ag-driven proliferation. because ifn-i production differs depending on the pathogen, we assessed cd8 t cell requirement for direct ifn-i signals during responses to vaccinia virus (vv), vesicular stomatitis virus (vsv), lymphocytic choriomeningitis virus (lcmv), and listeria monocytogenes (lm) immunizations in vivo. ifn-i-receptor-deficient (ifn-ir(o)) cd8 t cells expanded 3- to 5-fold less and formed a diminished memory pool ... | 2006 | 16849484 |
| the adenosine system selectively inhibits tlr-mediated tnf-alpha production in the human newborn. | human newborns are susceptible to microbial infection and mount poor vaccine responses, yet the mechanisms underlying their susceptibility are incompletely defined. we have previously reported that despite normal basal expression of tlrs and associated signaling intermediates, human neonatal cord blood monocytes demonstrate severe impairment in tnf-alpha production in response to triacylated (tlr 2/1) and diacylated (tlr 2/6) bacterial lipopeptides (blps). we now demonstrate that in marked contr ... | 2006 | 16849509 |
| modelling the individual cell lag time distributions of listeria monocytogenes as a function of the physiological state and the growth conditions. | the individual cell lag time distributions of listeria monocytogenes were characterized for 54 combinations of 22 initial physiological states, 18 growth conditions, and 11 strains. the individual cell lag times were deduced from the times for cultures issued from individual cells to reach an optical density threshold. the extreme value type ii distribution with a shape parameter set to 5 was shown effective to describe the 54 observed distributions. the theoretical distributions of individual l ... | 2006 | 16857284 |
| enhancement of mice susceptibility to infection with listeria monocytogenes by the treatment of morphine. | the effect of morphine on the susceptibility of balb/c mice to diarrheagenic escherichia coli, shigella flexneri, listeria monocytogenes, salmonella enteritidis, yersinia enterocolitica, was examined via the intraperitoneal inoculation. morphine treatment increased the susceptibility to s. enteritidis and l. monocytogenes, resulting in bacteremia and central nervous system (cns) invasion (for l. monocytogenes), while the infection with other bacteria did not show the systemic dissemination in th ... | 2006 | 16858145 |
| regulated translation of listeriolysin o controls virulence of listeria monocytogenes. | listeria monocytogenes is an intracytosolic bacterial pathogen that escapes from the phagosome using a secreted cytolysin, listeriolysin o (llo). in the host cytosol, llo activity is minimized to prevent pore formation in the host plasma membrane; premature lysis of the infected host cell exposes the bacteria to extracellular immune defences of the host and is detrimental to infection. here we identified nucleotide substitutions in the coding sequence of the llo gene (hly) that did not alter the ... | 2006 | 16859495 |
| vaccination with irradiated listeria induces protective t cell immunity. | we evaluated gamma-irradiated listeria monocytogenes as a killed bacterial vaccine, testing the hypothesis that irradiation preserves antigenic and adjuvant structures destroyed by traditional heat or chemical inactivation. irradiated listeria monocytogenes (lm), unlike heat-killed lm, efficiently activated dendritic cells via toll-like receptors and induced protective t cell responses in mice. like live lm, irradiated lm induced toll-like-receptor-independent t cell priming. cross-presentation ... | 2006 | 16860763 |
| antibacterial activity of bioconverted eicosapentaenoic (epa) and docosahexaenoic acid (dha) against foodborne pathogenic bacteria. | the antimicrobial activity of bioconversion extracts of epa and dha against a range of foodborne pathogenic bacteria was investigated. the bioconverted epa and dha exhibited antibacterial activities against four gram-positive bacteria, bacillus subtilis, listeria monocytogenes, staphylococcus aureus (atcc 6538) and s. aureus (kctc 1916) and seven gram-negative bacteria, enterobacter aerogenes, escherichia coli, e. coli o157:h7, e. coli o157:h7 (human), pseudomonas aeruginosa, salmonella enteriti ... | 2007 | 16860896 |
| isolation of listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction. | listeria monocytogenes, a gram-positive, facultative intracellular pathogen was isolated from buffaloes with a history of reproductive disorders and polymerase chain reaction (pcr) analyses for the presence of virulence-associated genes were conducted. a total of 530 samples of faecal, nasal, vaginal swabs and blood samples from 135 buffaloes were screened. the prevalence of l. monocytogenes and other listeria spp. was found to be 4.4 and 7.4%, respectively. all isolates were subjected to pcr fo ... | 2006 | 16860946 |
| the role of macrophage migration inhibitory factor in lethal listeria monocytogenes infection in mice. | macrophage migration inhibitory factor (mif) has been characterized as a proinflammatory cytokine. previous studies have indicated that mif may play a beneficial role or a detrimental role in microbial infections, depending on pathogens. in this study, we investigated the role of mif in listeria monocytogenes infection. the mif titers increased 6h after lethal l. monocytogenes infection but not in the sublethal infection. the elimination of bacteria from the spleens and livers was not affected b ... | 2006 | 16863687 |
| prevalence of listeria monocytogenes and other listeria species in butter from united kingdom production, retail, and catering premises. | two recent listeriosis outbreaks involving butter prompted this first cross-sectional study on the prevalence, levels, and types of listeria species in 3229 samples of butter from production, retail, and catering premises in the united kingdom during may and june 2004. when the criteria of the microbiological guidelines were used, 99.4% of samples were found to be of satisfactory microbiological quality, 0.5% were of acceptable quality, and 0.1% were of unsatisfactory quality as a result of high ... | 2006 | 16865880 |
| attachment of listeria monocytogenes to an austenitic stainless steel after welding and accelerated corrosion treatments. | austenitic stainless steels, widely used in food processing, undergo microstructural changes during welding, resulting in three distinctive zones: weld metal, heat-affected zone, and base metal. this research was conducted to determine the attachment of listeria monocytogenes in these three zones before and after exposure to a corrosive environment. all experiments were done with tungsten inert gas welding of type 304 stainless steel. the four welding treatments were large or small beads with hi ... | 2006 | 16865881 |
| survival of listeria monocytogenes in uncooked italian dry sausage (salami). | this study was undertaken to supplement existing information on the survival of listeria monocytogenes in italian salami. the fact that italian salami is frequently consumed by a large number of people poses some serious health implications. some raw materials have been found to be microbiologically contaminated, for their production occurs without any thermic treatment, and these are in circulation throughout italy all year round. we selected the product for its microbiological, technological, ... | 2006 | 16865882 |
| a predictive model to describe the effects of temperature, sodium lactate, and sodium diacetate on the inactivation of a serotype 4b strain of listeria monocytogenes in a frankfurter slurry. | a modified gompertz equation was used to model the effects of temperature (55, 60, and 65 degrees c), sodium lactate (0, 2.4, and 4.8%), and sodium diacetate (0, 0.125, and 0.25%) on inactivation of listeria monocytogenes strain mfs 102 (serotype 4b) in frankfurter slurry. the effects of these factors were determined on the shouldering region (parameter a), maximum death rate (parameter b), and tailing region (parameter c) of microbial inactivation curves. increased temperature or sodium diaceta ... | 2006 | 16865885 |
| effect of x-ray irradiation on reducing the risk of listeriosis in ready-to-eat vacuum-packaged smoked mullet. | listeria monocytogenes can pose a serious threat in several areas of the nation's food supply including ready-to-eat seafood products. use of irradiation processing can potentially reduce the risk of listeriosis caused by consumption of ready-to-eat seafood products. this study measured the effect of x-ray irradiation on reducing the population of l. monocytogenes on ready-to-eat, vacuum-packaged smoked mullet. smoked mullet were inoculated with a five-strain mixture of l. monocytogenes (10(4) c ... | 2006 | 16865886 |
| efficacy of disinfectants to reduce listeria monocytogenes on precut iceberg lettuce. | the efficacy of water, chlorinated water (100 ppm), peracetic acid solution (0.05%), and commercial citric acid-based produce wash (0.25%) to reduce the population of listeria monocytogenes on precut lettuce was tested. samples were inoculated with a mixture of equal amounts of five l. monocytogenes strains at a level of 4.7 log cfu/g, and analyzed on the day of washing and after 3 and 6 days of storage at 6 degrees c. sanitizer reduced the number of l. monocytogenes at maximum 1.7 log cfu/g and ... | 2006 | 16865887 |
| adhesion properties and competitive pathogen exclusion ability of bifidobacteria with acquired acid resistance. | the adhesion properties of bifidobacterium longum and bifidobacterium catenulatum strains with an acquired resistance to acid and their ability to competitively exclude salmonella enterica serovar typhimurium, escherichia coli, listeria monocytogenes, enterobacter sakazakii, and clostridium difficile from adhering to human intestinal mucus were evaluated and compared with the results when the same experiments were run with the original acid-sensitive strains. in half of the four studied cases, t ... | 2006 | 16865903 |
| molecular epidemiology and cluster analysis of human listeriosis cases in three u.s. states. | to better understand the transmission and epidemiology of human listeriosis, 647 listeria monocytogenes isolates obtained from human listeriosis cases in four u.s. locations (michigan, ohio, new york state, and new york city) over 61 months (1998 to 2003) were characterized by automated ecori ribotyping. a total of 65 ribotypes were differentiated among the characterized isolates; 393, 227, and 24 isolates were classified into lineages i, ii, and iii, respectively, and 3 isolates were not classi ... | 2006 | 16865904 |
| antimicrobial activity of extracts of the lichen xanthoparmelia pokornyi and its gyrophoric and stenosporic acid constituents. | the antimicrobial activity of the diethyl ether, acetone, chloroform, petroleum ether, and ethanol extracts of the lichen xanthoparmelia pokornyi and its gyrophoric acid and stenosporic acid constituents has been screened against some foodborne bacteria and fungi. both the extracts and the acids showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, staphylococcus aureus, streptococcus faecalis, yersinia enterocol ... | 2006 | 16869486 |
| survival of listeria monocytogenes and enterococcus faecium in sludge evaluated by real-time pcr and culture methods. | this study evaluates the behaviour in spiked sludge of a pathogenic bacteria, listeria monocytogenes, by cultural and molecular techniques, and compares its survival with the one of a faecal indicator, enterococcus faecium. | 2006 | 16869894 |
| effects of acidified sodium chlorite, cetylpyridinium chloride and hot water on populations of listeria monocytogenes and staphylococcus aureus on beef. | the present study was designed to determine the individual and combined effects of acidified sodium chlorite (asc, 0.1%, 24 +/- 1 degrees c), cetylpyridinium chloride (cpc, 0.5%, 24 +/- 1 degrees c) and hot water (hw, 93 +/- 1 degrees c) treatments on the survival of listeria monocytogenes and staphylococcus aureus. | 2006 | 16869900 |
| contribution of penicillin-binding protein homologs to antibiotic resistance, cell morphology, and virulence of listeria monocytogenes egde. | seven open reading frames, annotated as potential penicillin-binding-protein-encoding genes (lmo0441, lmo0540, lmo1438, lmo1892, lmo2039, lmo2229, and lmo2754), were targeted for insertional mutagenesis in listeria monocytogenes egde. these genes were found to contribute in various degrees to beta-lactam resistance, cell morphology, or the virulence potential of this organism. | 2006 | 16870778 |
| safety of etanercept in psoriasis: a critical review. | conventional systemic treatments for patients with psoriasis are associated with multiple adverse effects that require continuous monitoring. the introduction of new biological agents such as etanercept, a fully human fusion protein, has permitted individualisation of patients' treatment according to disease stage. the drug is a competitive inhibitor of tumour necrosis factor-alpha (tnfalpha) that prevents interaction between this cytokine and its cell surface receptors. etanercept also modulate ... | 2006 | 16872241 |
| detection of antimicrobial activities and bacteriocin structural genes in faecal enterococci of wild animals. | the production of antimicrobial activities as well as the presence of bacteriocin structural genes (enta, entb, entp, entq, cyll, entas-48, bac31, and entl50a/b) were studied in 140 non-selected faecal enterococcal isolates recovered from wild animals. eight different indicator strains (including listeria monocytogenes, pediococcus pentosaceus, and different enterococcal species) were used for antimicrobial activity detection. twenty-five of the 140 enterococci (18%) showed antimicrobial activit ... | 2007 | 16872815 |
| comparison of listeria monocytogenes strain types in irish smoked salmon and other foods. | an investigation of listeria monocytogenes in irish retail smoked salmon products and other unrelated food products was undertaken. serotyping and genotyping methods were applied. twenty-six l. monocytogenes isolates cultured from ready-to-eat smoked salmon and an additional 20 l. monocytogenes isolates from various commercially available food products (other than smoked salmon) were compared. four serotypes, 12 ribotypes, 12 amplified fragment length polymorphism (aflp) types and 17 pulsed-fiel ... | 2006 | 16872895 |
| cytoplasmic bacteria and the autophagic pathway. | cytoplasmic bacteria may assist in our study of the autophagic pathway. this review highlights the use of listeria monocytogenes for examining the assembly of autophagic vacuoles in mammalian cells. inhibiting protein synthesis of cytoplasmic l. monocytogenes results in their being sequestered into the autophagic pathway. autophagic vacuoles form around the easily identified bacterial particles making the assembly process easy to study using morphological and biochemical methods. l. monocytogene ... | 2006 | 16874112 |
| survival of listeria monocytogenes scott a on metal surfaces: implications for cross-contamination. | listeria monocytogenes is an important re-emerging pathogen which is commonly found in the environment. many outbreaks have been associated with the contamination of food produce, often linked to cross-contamination from surfaces or equipment to prepared foodstuffs. in the present study a number of copper-base metal alloys have been used to assess the survival times of l. monocytogenes on different materials, in comparison with stainless steel. high concentrations (10(7)) of bacteria were placed ... | 2006 | 16876278 |