Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
functional characterization of alpha-synuclein protein with antimicrobial activity.alpha-synuclein (α-syn), a small (14 kda) protein associated with parkinson's disease, is abundant in human neural tissues. α-syn plays an important role in maintaining a supply of synaptic vesicles in presynaptic terminals; however, the mechanism by which it performs this function are not well understood. in addition, there is a correlation between α-syn over-expression and upregulation of an innate immune response. given the growing body of literature surrounding antimicrobial peptides (amps) ...201627520375
role of aspergillus fumigatus-specific igg in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis.few studies have evaluated the utility of aspergillus fumigatus-specific igg in allergic bronchopulmonary aspergillosis (abpa). herein, we evaluate the role of specific igg in diagnosis and monitoring treatment response in abpa. forty-eight control subjects with a. fumigatus-associated asthma underwent a. fumigatus-specific igg measurements at baseline, while specific igg was assayed in 102 treatment-naïve subjects of abpa at baseline, after eight weeks of glucocorticoid therapy, and during exac ...201727523578
preparation, characterization and antimicrobial applications of zn-fe ldh against mrsa.facile and simple processes to get zn-fe layered double hydroxide (ldh) with nitrate as the interlayer anion are reported. the method of co-precipitation produced high crystallinity ldh that is marked by xrd, sem, tem and ft-ir. results showed that 99.8% of cd(+2) removals were at ph11 and 4h. to get the adsorption isotherms, the concentration of metal ions extending from 6 to 18mg/l was utilized. results supported the langmuir adsorption model. in contrary, the adsorption process followed the p ...201627524011
direct molecular diagnosis of aspergillosis and cyp51a profiling from respiratory samples of french patients.microbiological diagnosis of aspergillosis and triazole resistance is limited by poor culture yield. to better estimate this shortcoming, we compared culture and molecular detection of a. fumigatus in respiratory samples from french patients at risk for aspergillosis.201627524978
pcr-based detection of aspergillus fumigatus cyp51a mutations on bronchoalveolar lavage: a multicentre validation of the aspergenius assay® in 201 patients with haematological disease suspected for invasive aspergillosis.in patients with invasive aspergillosis (ia), fungal cultures are mostly negative. consequently, azole resistance often remains undetected. the aspergenius(®) multiplex real-time pcr assay identifies clinically relevant aspergillus species and four resistance-associated mutations (rams; tr34/l98h/t289a/y121f) in the cyp51a gene. this multicentre study evaluated the diagnostic performance of this assay on bronchoalveolar lavage (bal) fluid and correlated the presence of rams with azole treatment ...201627530755
the aspergillus fumigatus scha(sch9) kinase modulates saka(hog1) map kinase activity and it is essential for virulence.the serine-threonine kinase tor, the target of rapamycin, is an important regulator of nutrient, energy and stress signaling in eukaryotes. sch9, a ser/thr kinase of agc family (the camp-dependent pka, cgmp- dependent protein kinase g and phospholipid-dependent protein kinase c family), is a substrate of tor. here, we characterized the fungal opportunistic pathogen aspergillus fumigatus sch9 homologue (scha). the scha null mutant was sensitive to rapamycin, high concentrations of calcium, hypero ...201627538790
der f 34, a novel major house dust mite allergen belonging to a highly conserved rid/yjgf/yer057c/uk114 family of imine deaminases.the high prevalence of house dust mite (hdm) allergy is a growing health problem worldwide, and the characterization of clinically important hdm allergens is a prerequisite for the development of diagnostic and therapeutic strategies. here, we report a novel hdm allergen that belongs structurally to the highly conserved rid/yjgf/yer057c/uk114 family (rid family) with imine deaminase activity. isolated hdm cdna, named der f 34, encodes 128 amino acids homologous to rid-like proteins. this new pro ...201627539850
emerging aspergillosis by azole-resistant aspergillus fumigatus at an intensive care unit in the netherlands, 2010 to 2013.the prevalence of invasive aspergillosis (ia) at the intensive care unit (icu) is unknown and difficult to assess since ia also develops in patients lacking specific host factors. in the netherlands, increasing azole-resistance in aspergillus fumigatus complicates treatment of patients with ia. the aim of this study was to determine the prevalence of ia by azole-resistant a. fumigatus at the icu among patients receiving antifungal treatment and to follow their clinical outcome and prognosis. a r ...201627541498
aspergillus fumigatus components distinguish ige but not igg4 profiles between fungal sensitization and allergic broncho-pulmonary aspergillosis.aspergillus fumigatus is the causative agent of allergic broncho-pulmonary aspergillosis. prompt and accurate diagnosis may be difficult to achieve with current clinical and laboratory scores, which do not include immune responses to recombinant a. fumigatus allergens. we measured specific immunoglobulin e and g4 directed to recombinant a. fumigatus allergens in 55 cystic fibrosis patients without allergic broncho-pulmonary aspergillosis but sensitized to a. fumigatus and in nine patients with a ...201627542151
phytochemical and cytogenetic characterization of centaurea solstitialis l. (asteraceae) from croatia.the cytogenetic characterization of centaurea solstitialis l. (asteraceae) showed a chromosome number of 2n = 16. karyotype is composed by four pairs of metacentric, two pairs of submetacentric and two pairs of subtelocentric chromosomes. physical mapping of two rdna probes revealed two loci of 35s and one locus of 5s rrna genes. chromomycin fluorochrome banding revealed that all rdna loci were gc rich. the genome size (2c-value) of 1.95 pg classes this species in the group of very small genomes ...201727552682
high frequency of pathogenic aspergillus species among nonsporulating moulds from respiratory tract samples.nonsporulating moulds (nsm) represent an identification challenge for clinical laboratories. data on the prevalence of pathogenic species among nsm are lacking. we prospectively investigated consecutive thermotolerant (36°c) clinical nsm isolates from respiratory tract samples. a total of 123 isolates were identified by dna sequencing and phenotypically characterized. of those, 13 (11%) were pathogenic species (aspergillus fumigatus, n = 10; a. flavus, n = 1; a. hiratsukae, n = 1; schizophyllum ...201727555561
how neutrophils kill fungi.neutrophils play a critical role in the prevention of invasive fungal infections. whereas mouse studies have demonstrated the role of various neutrophil pathogen recognition receptors (prrs), signal transduction pathways, and cytotoxicity in the murine antifungal immune response, much less is known about the killing of fungi by human neutrophils. recently, novel primary immunodeficiencies have been identified in patients with a susceptibility to fungal infections. these human 'knock-out' neutrop ...201627558342
[cross-reactivity in allergic fungal sinusitis. case report].the presence of allergic mucin in allergic fungal sinusitis (afs) is a manifestation that identifies it as a hypersensitivity process. afs has a phenomenon of cross-reactivity to ige-bound proteins having at least two shared epitopes.201627560920
acorus calamus linn.: phytoconstituents and bactericidal property.acorus calamus linn. of the family araceae (acoraceae), commonly known as sweet flag and vacha. the rhizome of this plant has medicinal properties against bugs, moths, lice and emetic stomach in dyspepsia. chemical composition of the hydro-distilled essential oil obtained from the rhizomes of a. calamus was analyzed by gas chromatography equipped with flame ionization detector and gas chromatography coupled with mass spectrometry. the essential oil of a. calamus and its major compound β-asarone ...201627562598
potent activities of novel imidazoles lanoconazole and luliconazole against a collection of azole-resistant and -susceptible aspergillus fumigatus strains.a collection of azole-susceptible (n = 141) and azole-resistant (n = 27) aspergillus fumigatus isolates was tested against seven antifungal drugs, including the new imidazoles lanoconazole and luliconazole. the luliconazole and lanoconazole mic90 values for the azole-susceptible strains were 0.001 μg/ml and 0.008 μg/ml, and those for the azole-resistant strains were 0.016 μg/ml and 0.032 μg/ml.201627572389
discovery of a new antifungal agent asp2397 using a silkworm model of aspergillus fumigatus infection.natural products are the major source of currently available drugs. however, screening natural product presents several challenges, including the time-consuming and labor-intensive steps required for the isolation of a drug from crude extracts as well as the differences between the activities of compounds in vitro and in vivo. to address these challenges, we used silkworm larvae infected with aspergillus fumigatus to screen a natural products library for potent drugs to treat invasive aspergillo ...201727577982
the current status of drug-resistant aspergillus.aspergillus spp. are important fungi because of the high mortality associated with aspergillosis. however, we have only three classes of anti-aspergillus drugs available; and only two drugs, itraconazole (itcz) and voriconazole (vrcz), are available as oral agents in japan. if azole-resistant aspergillus causes chronic aspergillosis, we cannot treat such patients with oral azoles on an outpatient basis. as is the case with foreign countries, we found the existence of azole-resistant aspergillus ...201627581778
characterization of gprk encoding a putative hybrid g-protein-coupled receptor in aspergillus fumigatus.the g-protein-coupled receptor (gpcr) family represents the largest and most varied collection of membrane embedded proteins that are sensitized by ligand binding and interact with heterotrimeric g proteins. despite their presumed critical roles in fungal biology, the functions of the gpcr family members in the opportunistic human pathogen aspergillus fumigatus are largely unknown, as only two (gprc and gprd) of the 15 predicted gpcrs have been studied. here, we characterize the gprk gene, which ...201627584150
allergic bronchopulmonary aspergillosis: alla radiologist needs to know.allergic bronchopulmonary aspergillosis (abpa) is an immunological lung disorder occurring due to hypersensitivity to fungus aspergillus fumigatus. early diagnosis of abpa is desirable as the disease can cause irreversible damage and end stage lung disease. diagnosis of abpa is based upon a set of clinical, serological and radiological criteria. radiology plays an important role in the diagnosis of abpa. however a radiologist should also be familiar with various clinical and serological criteria ...201627585455
inhibition of the flavin-dependent monooxygenase siderophore a (sida) blocks siderophore biosynthesis and aspergillus fumigatus growth.aspergillus fumigatus is an opportunistic fungal pathogen and the most common causative agent of fatal invasive mycoses. the flavin-dependent monooxygenase siderophore a (sida) catalyzes the oxygen and nadph dependent hydroxylation of l-ornithine (l-orn) to n(5)-l-hydroxyornithine in the biosynthetic pathway of hydroxamate-containing siderophores in a. fumigatus. deletion of the gene that codes for sida has shown that it is essential in establishing infection in mice models. here, a fluorescence ...201627588426
aspergillus fumigatus carrying tr34/l98h resistance allele causing complicated suppurative otitis media in tanzania: call for improved diagnosis of fungi in sub-saharan africa.suppurative otitis media (som) is a major public health concern worldwide and is associated with increased morbidity. cases of fungal suppurative otitis media were studied to establish the effect of fungi in otitis media.201627589956
demethylase inhibitor fungicide resistance in pyrenophora teres f. sp. teres associated with target site modification and inducible overexpression of cyp51.pyrenophora teres f. sp. teres is the cause of net form of net blotch (nfnb), an economically important foliar disease in barley (hordeum vulgare). net and spot forms of net blotch are widely controlled using site-specific systemic fungicides. although resistance to succinate dehydrogenase inhibitors and quinone outside inhibitors has been addressed before in net blotches, mechanisms controlling demethylation inhibitor resistance have not yet been reported at the molecular level. here we report ...201627594852
allergic bronchopulmonary aspergillosis in asthma. 201727599560
are allergic fungal rhinosinusitis and allergic bronchopulmonary aspergillosis lifelong conditions?aspergillus fumigatus can cause several allergic disorders including aspergillus-sensitized asthma, allergic bronchopulmonary aspergillosis (abpa), and allergic fungal rhinosinusitis (afrs). abpa is an immunological pulmonary disorder caused by allergic reactions mounted against antigens of a. fumigatus colonizing the airways of patients with asthma (and cystic fibrosis). allergic bronchopulmonary mycosis is an allergic fungal airway disease caused by thermotolerant fungi other than a. fumigatus ...201727601608
new phytochemical constituent and bioactivities of horwoodia dicksoniae and rumex cyprius.many plants growing in saudi arabia are used in folk medicine for treatment of several diseases.201727601844
biosynthesis of cell wall mannan in the conidium and the mycelium of aspergillus fumigatus.the galactomannan is a major cell wall molecule of aspergillus fumigatus. this molecule is composed of a linear mannan with a repeating unit composed of four α1,6 and α1,2 linked mannose with side chains of galactofuran. to obtain a better understanding of the mannan biosynthesis in a. fumigatus, it was decided to undertake the successive deletion of the 11 genes which are putative orthologs of the mannosyltransferases responsible for establishing α1,6 and α1,2 mannose linkages in yeast. these d ...201627603677
discrimination of aspergillosis, mucormycosis, fusariosis, and scedosporiosis in formalin-fixed paraffin-embedded tissue specimens by use of multiple real-time quantitative pcr assays.in a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (ffpe) tissue specimens with histopathology results were tested. two 4- to 5-μm ffpe tissue sections from each specimen were digested with proteinase k, followed by automated nucleic acid extraction. multiple real-time quantitative pcr (qpcr) assays targeting the internal transcribed spacer 2 (its2) region of ribosomal dna, using fluorescently labeled primers, was performed to identify clinically important genera and spec ...201627605714
30 years of battling the cell wall.in aspergillus fumigatus, like in other pathogenic fungi, the cell wall is essential for fungal growth as well as for resisting environmental stresses such as phagocytic killing. most of the chemical analyses undertaken on the cell wall of a. fumigatus are focused on the mycelial cell wall because it is the vegetative stage of the fungus. however, the cell walls of the mycelium and conidium (which is the infective propagule) are different especially at the level of the surface layer, which plays ...201727609562
aspergillus arthritis: analysis of clinical manifestations, diagnosis, and treatment of 31 reported cases.aspergillus arthritis is a debilitating form of invasive aspergillosis. little is known about its epidemiology, clinical manifestations, laboratory features, treatment, and prognosis. cases of aspergillus arthritis were reviewed in the english literature from 1967 through 2015 for variables of arthritis with aspergillus spp. recovered from joint and/or adjacent bone, underlying conditions, symptoms, signs, inflammatory biomarkers, diagnostic imaging, management, and outcome. among 31 evaluable c ...201627609563
functional reconstitution of a fungal natural product gene cluster by advanced genome editing.filamentous fungi produce varieties of natural products even in a strain dependent manner. however, the genetic basis of chemical speciation between strains is still widely unknown. one example is trypacidin, a natural product of the opportunistic human pathogen aspergillus fumigatus, which is not produced among different isolates. combining computational analysis with targeted gene editing, we could link a single nucleotide insertion in the polyketide synthase of the trypacidin biosynthetic pat ...201727611015
ligand based pharmacophoric modelling and docking of bioactive pyrazolium 3-nitrophthalate (p3np) on bacillus subtilis, aspergillus fumigatus and aspergillus niger - computational and hirshfeld surface analysis.biologically active lewis acid-base compound, pyrazolium 3-nitro phthalate (p3np) has been synthesized and crystallized by slow evaporation - solution method at 30°c. spectral and single crystal x-ray diffraction (xrd) were used to characterize the compound. the stability of the p3np was confirmed by uv-visible spectral analysis. p3np crystallizes in monoclinic p21/c space group with cell parameters, a=13.009 (3) å, b=12.584 (3) å, c=7.529 (18) å and β=93.052 (4)(o) with z=4. crystal packing was ...201627614246
insight into enzymatic degradation of corn, wheat, and soybean cell wall cellulose using quantitative secretome analysis of aspergillus fumigatus.lignocelluloses contained in animal forage cannot be digested by pigs or poultry with 100% efficiency. on contrary, aspergillus fumigatus, a saprophytic filamentous fungus, is known to harbor 263 glycoside hydrolase encoding genes, suggesting that a. fumigatus is an efficient lignocellulose degrader. hence the present study uses corn, wheat, or soybean as a sole carbon source to culture a. fumigatus under animal physiological condition to understand how cellulolytic enzymes work together to achi ...201627618962
dataset of differentially regulated proteins in huvecs challenged with wild type and ugm1 mutant aspergillus fumigatus strains.invasive aspergillosis is the primary opportunistic invasive fungal infection described in neutropenic hematologic patients, caused by the angioinvasive pathogen aspergillus fumigatus. the molecular mechanisms associated with a. fumigatus infection in the vascular endothelium are poorly understood. in this context, we used a high-throughput proteomic approach to unveil the proteins modulated in huvecs after interaction with a wild type strain and the ugm1 mutant (δugm1) of a. fumigatus. the prot ...201627622208
the crystal structure of peroxiredoxin asp f3 provides mechanistic insight into oxidative stress resistance and virulence of aspergillus fumigatus.invasive aspergillosis and other fungal infections occur in immunocompromised individuals, including patients who received blood-building stem cell transplants, patients with chronic granulomatous disease (cgd), and others. production of reactive oxygen species (ros) by immune cells, which incidentally is defective in cgd patients, is considered to be a fundamental process in inflammation and antifungal immune response. here we show that the peroxiredoxin asp f3 of aspergillus fumigatus inactiva ...201627624005
aspergillus pseudomembranous tracheobronchitis in an immunocompetent individual: a diagnostic conundrum with therapeutic challenge.aspergillus tracheobronchitis is an extremely uncommon manifestation of aspergillus infection. most of the cases described in the literature are in the immunosuppressed individuals and is almost uniformly fatal. immunocompetent individuals do manifest the disease, but the disease if diagnosed early can be appropriately treated and thus can be life-saving. here, we describe a similar case which was diagnosed only at autopsy.201727625454
a high throughput method for rapid screening of chitosanase-producing fungal strain under acidic conditions.a novel high-throughput method was established for rapid screening of a large numbers of aspergillus fumigatus (a. fumigatus) mutants with high chitosanase production under acidic culture condition by exploiting the fact that iodine can be used as the indicator to stain chitosan but is ineffective for chitooligosaccharides. the mutant population was generated by irradiating a. fumigatus cicc 2434 with co(60)-γ rays. mutants were cultured on acidic plates containing colloidal chitosan and prelimi ...201627628334
in-host adaptation and acquired triazole resistance in aspergillus fumigatus: a dilemma for clinical management.aspergillus fumigatus causes a range of diseases in human beings, some of which are characterised by fungal persistence. a fumigatus can persist by adapting to the human lung environment through physiological and genomic changes. the physiological changes are based on the large biochemical versatility of the fungus, and the genomic changes are based on the capacity of the fungus to generate genetic diversity by spontaneous mutations or recombination and subsequent selection of the genotypes that ...201627638360
modified release itraconazole amorphous solid dispersion to treat aspergillus fumigatus: importance of the animal model selection.previously, modified release itraconazole in the form of a melt-extruded amorphous solid dispersion based on a ph dependent enteric polymer combined with hydrophilic additives (hme-itz), exhibited improved in vitro dissolution properties. these properties agreed with pharmacokinetic results in rats showing high and sustained itraconazole (itz) systemic levels. the objective of the present study was to better understand the best choice of rodent model for evaluating the pharmacokinetic and effica ...201727645428
diagnosis of invasive pulmonary aspergillosis.culturing strains from clinical samples is the main method to diagnose invasive pulmonary aspergillosis. detecting the galactomannan antigen in serum samples is an auxiliary examination. the goal of this study was to determine the frequency with which aspergillus fumigatus was cultured in clinical samples taken from patients hospitalized in the the infant jesus teaching hospital in warsaw, poland, in the period of 2013-2014. specimens from the respiratory tract and blood were cultured for mycolo ...201627645542
scf ubiquitin ligase f-box protein fbx15 controls nuclear co-repressor localization, stress response and virulence of the human pathogen aspergillus fumigatus.f-box proteins share the f-box domain to connect substrates of e3 scf ubiquitin ring ligases through the adaptor skp1/a to cul1/a scaffolds. f-box protein fbx15 is part of the general stress response of the human pathogenic mold aspergillus fumigatus. oxidative stress induces a transient peak of fbx15 expression, resulting in 3x elevated fbx15 protein levels. during non-stress conditions fbx15 is phosphorylated and f-box mediated interaction with skpa preferentially happens in smaller subpopulat ...201627649508
aspergillus fumigatus photobiology illuminates the marked heterogeneity between isolates.the given strain of aspergillus fumigatus under study varies across laboratories, ranging from a few widely used "standards," e.g., af293 or cea10, to locally acquired isolates that may be unique to one investigator. since experiments concerning physiology or gene function are seldom replicated by others, i.e., in a different a. fumigatus background, the extent to which behavioral heterogeneity exists within the species is poorly understood. as a proxy for assessing such intraspecies variability ...201627651362
heterogeneity among isolates reveals that fitness in low oxygen correlates with aspergillus fumigatus virulence.previous work has shown that environmental and clinical isolates of aspergillus fumigatus represent a diverse population that occupies a variety of niches, has extensive genetic diversity, and exhibits virulence heterogeneity in a number of animal models of invasive pulmonary aspergillosis (ipa). however, mechanisms explaining differences in virulence among a. fumigatus isolates remain enigmatic. here, we report a significant difference in virulence of two common lab strains, cea10 and af293, in ...201627651366
the putative flavin carrier family flca-c is important for aspergillus fumigatus virulence.aspergillus fumigatus is an opportunistic fungal pathogen and the most important species causing pulmonary fungal infections. the signaling by calcium is very important for a. fumigatus pathogenicity and it is regulated by the transcription factor crza. we have previously used used chip-seq (chromatin immunoprecipitation dna sequencing) aiming to identify gene targets regulated by crza. we have identified among several genes regulated by calcium stress, the putative flavin transporter, flca. thi ...201627652896
determination of antifungal susceptibility patterns among the environmental isolates of aspergillus fumigatus in iran.in recent years, triazole-resistant environmental isolates of aspergillus fumigatus have emerged in europe and asia. azole resistance has been reported in patients who are treated with long-term azole therapy or exposure of the fungus spores to the azole fungicides used in agriculture. to date, a wide range of mutations in a. fumigatus have been described conferring azole-resistance, which commonly involves modifications in the cyp51a gene. we investigated antifungal susceptibility pattern of en ...201627656605
antifungal activity of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides: a novel type of compound afforded by azide-enolate (3+2) cycloaddition.the first report of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides (7a and 7b) is described herein. azide-enolate (3+2) cycloaddition afforded the synthesis of this novel type of compound. antifungal activity was evaluated in vitro against four filamentous fungi (aspergillus fumigatus, trichosporon cutaneum, rhizopus oryzae and mucor hiemalis) as well as nine species of candida spp. as yeast specimens. these pre-clinical studies suggest that compounds 7a and 7b are promising candidates for co ...201627656774
a rare case of peripheral vascular graft infection by aspergillus fumigatus and review of the literature.we present an unusual case of a 74-year-old woman who presented with a pseudoaneurysm at the site of a previous prosthetic femoro-popliteal bypass graft and underwent an urgent repair with pseudoaneurysm excision and extra-anatomic placement of an autologous venous graft. microbiology examination revealed aspergillus fumigatus infection of the graft, which is a very rare condition. long-term antifungal medication has been administrated with positive outcome. only a few cases have been reported i ...201727659379
aspergillus fumigatus dbm 4057 biofilm formation is inhibited by chitosan, in contrast to baicalein and rhamnolipid.the biofilms of filamentous-forming fungi are a novel and still insufficiently understood research topic. we have studied aspergillus fumigatus, an ubiquitous opportunistic pathogenic fungus, as a representative model for a study of biofilm formation by filamentous fungi and for assessing the potential anti-biofilm activity of natural substances. the activity of antibiotic amphotericin b and selected natural substances: baicalein, chitosan and rhamnolipid was studied. the minimum suspension inhi ...201627660214
exposure to positively- and negatively-charged plasma cluster ions impairs ige-binding capacity of indoor cat and fungal allergens.environmental control to reduce the amount of allergens in a living place is thought to be important to avoid sensitization to airborne allergens. however, efficacy of environmental control on inactivation of airborne allergens is not fully investigated. we have previously reported that positively- and negatively-charged plasma cluster ions (pc-ions) reduce the ige-binding capacity of crude allergens from japanese cedar pollen as important seasonal airborne allergens. cat (felis domesticus) and ...201627660668
genotypic discrimination of aspergillus fumigatus strain from related species within section fumigati.the aim was to make an exact diagnosis of 20 strains using molecular biological methods which were isolated from the atmosphere of the inpatient rooms in the oncology and other departments of the ege university medical faculty hospital, and identified as aspergillus fumigatus through phenotypic tests, and to determine their antibiotic susceptibility patterns.201627660866
synthesis, characterization, and biological activity of cross-linked chitosan biguanidine loaded with silver nanoparticles.chitosan biguanidine hydrochloride (chg) and glutaraldehyde cross-linked chitosan biguanidine (cchg) were synthesized and characterized by fourier transform infrared spectroscopy, (1)h nmr and (13)c nmr, x-ray diffraction, scanning electron microscopy (sem) and thermal analyses (tga and dta). the results showed that chg and cchg had a more amorphous structure than that of chitosan, and their thermal stability were slightly lower than that of chitosan. colloidal silver nanoparticles (agnps) were ...201627662448
performance evaluation of multiplex pcr including aspergillus-not so simple!multiplex pcrs have been designed for including species other than aspergillus fumigatus for the diagnosis of invasive aspergillosis, such as microarrays, liquid-phase array, and electrospray-ionization mass spectrometry (pcr/esi ms). these methods are based on the selection of multiple primers to amplify different species with the specificity checked by hybridization to a probe or by base composition of the amplicon for the pcr/esi ms. when testing complex samples such as respiratory specimens, ...201727664168
phytogenic synthesis of silver nanoparticles, optimization and evaluation of in vitro antifungal activity against human and plant pathogens.an attempt was made to synthesis of biocompatible silver nanoparticles from ten different cassia spp. among them, cassia roxburghii aqueous leaf extract supported the synthesis of highly efficient and stable agnps. the synthesis of agnps was optimized at different physico-chemical condition and highly stable agnps were synthesized with 1.0ml of c. roxburghii leaf extract, ph 7.0, 1.0mm agno3 and at 37°c. the synthesized agnps were characterized by xps, dls and zeta potential. dls and zeta potent ...201627664723
transcript levels of the aspergillus fumigatus cdc42 module, polarisome, and septin genes show little change from dormancy to polarity establishment.aspergillus fumigatus is the most common airborne pathogen causing fatal mycoses in immunocompromised patients. during the first 8 hours of development a. fumigatus conidia break dormancy, expand isotopically, establish an axis of polarity, and begin to extend germ tubes in a polar manner. the transition from isotropic to polar growth is critical for tissue invasion and pathogenesis. in the current work, we used two-color microarrays to examine the a. fumigatus transcriptome during early develop ...201627664992
prevalence of azole-resistant aspergillus fumigatus in the environment of thailand.occurrence of azole-resistant aspergillus fumigatus (araf) in the environment is an emerging problem worldwide, likely impacting on patient treatment. several resistance mutations are thought to have initially arisen through triazole-based fungicide use in agriculture and subsequently being propagated in a similar manner. here we investigated the prevalence of araf in the environment of thailand and characterized their susceptibility profiles toward clinically used azole compounds along with und ...201627664994
could we predict airborne aspergillus contamination during construction work?aspergillus fumigatus is a major opportunistic pathogen causing nosocomial infection. hospital outbreaks of invasive aspergillosis have been associated with demolition and building construction. this study was designed to examine the impact of meteorologic factors and different periods of work on outdoor fungal airborne concentrations.201727665035
the role of lox-1 on innate immunity against aspergillus keratitis in mice.to explore the effects of lectin-like ox-ldl receptor (lox-1) on innate immunity against aspergillus fumigatus (a. fumigatus ) in mice cornea.201627672585
unveiling the synergistic interaction between liposomal amphotericin b and colistin.patients with multiple comorbidities are often administered simultaneously or sequentially antifungals and antibacterial agents, without full knowledge of the consequences of drug interactions. considering the clinical relevance of liposomal amphotericin b (l-amb), the association between l-amb and six antibacterial agents was evaluated against four clinical isolates and one type strain of candida spp. and two clinical isolates and one type strain of aspergillus fumigatus. in order to evaluate s ...201627679618
home environment as a source of life-threatening azole-resistant aspergillus fumigatus in immunocompromised patients.a case of fatal aspergillosis due to a tr46/y121f/t289a azole-resistant aspergillus fumigatus is reported. environmental investigations at the patient's residence led to the recovery of tr46/y121f/t289a isolates, genotypically indistinguishable from the clinical isolate, supporting for the first time the direct role of household as potential source of azole-resistant invasive aspergillosis.201727682064
aspergillosis and stem cell transplantation: an overview of experimental pathogenesis studies.invasive aspergillosis is a life-threatening infection caused by the opportunistic filamentous fungus aspergillus fumigatus. patients undergoing haematopoietic stem cell transplant (hsct) for the treatment of hematological malignancy are at particularly high risk of developing this fatal infection. the susceptibility of hsct patients to infection with a. fumigatus is a consequence of a complex interplay of both fungal and host factors. here we review our understanding of the host-pathogen intera ...201627687755
bioaerosols in the barcelona subway system.subway systems worldwide transport more than 100 million people daily; therefore, air quality on station platforms and inside trains is an important urban air pollution issue. we examined the microbiological composition and abundance in space and time of bioaerosols collected in the barcelona subway system during a cold period. quantitative pcr was used to quantify total bacteria, aspergillus fumigatus, influenza a and b, and rhinoviruses. multitag 454 pyrosequencing of the 16s rrna gene was use ...201627687789
lox-1 and tlr4 affect each other and regulate the generation of ros in a. fumigatus keratitis.to explore the relationship between lox-1 and tlr4 in aspergillus fumigatus (a. fumigatus) keratitis. to determine lox-1 and tlr4 can affect each other and regulate inflammation through regulation of the generation of reactive oxygen species (ros) in a. fumigatus keratitis.201627694040
regulation of secondary metabolism by the velvet complex is temperature-responsive in aspergillus.sensing and responding to environmental cues is critical to the lifestyle of filamentous fungi. how environmental variation influences fungi to produce a wide diversity of ecologically important secondary metabolites (sms) is not well understood. to address this question, we first examined changes in global gene expression of the opportunistic human pathogen, aspergillus fumigatus, after exposure to different temperature conditions. we found that 11 of the 37 sm gene clusters in a. fumigatus wer ...201627694115
resistance in human pathogenic yeasts and filamentous fungi: prevalence, underlying molecular mechanisms and link to the use of antifungals in humans and the environment.antifungal drug resistance is a multifaceted clinical challenge, and when present, a primary cause of treatment failure in patients with severe fungal infections. changing epidemiology, increasing resistance rates and a narrow antifungal armamentarium may further underline the required attention on resistance particularly within the most prevalent invasive fungal infections caused by candida yeasts and aspergillus moulds. in denmark, the resistance epidemiology remains to be fully elucidated. th ...201627697142
coinfection by cetacean morbillivirus and aspergillus fumigatus in a juvenile bottlenose dolphin (tursiops truncatus) in the gulf of mexico.a recently deceased juvenile male bottlenose dolphin (tursiops truncatus) was found floating in the gulf of mexico, off sand key in clearwater, florida. at autopsy, we identified pneumonia and a focus of malacia in the right cerebrum. cytologic evaluation of tissue imprints from the right cerebrum revealed fungal hyphae. fungal cultures of the lung and brain yielded aspergillus fumigatus, which was confirmed by amplification of a portion of the fungal nuclear ribosomal internal transcribed space ...201627698174
a new aspergillus fumigatus typing method based on hypervariable tandem repeats located within exons of surface protein coding genes (tresp).aspergillus fumigatus is a saprotrophic mold fungus ubiquitously found in the environment and is the most common species causing invasive aspergillosis in immunocompromised individuals. for a. fumigatus genotyping, the short tandem repeat method (straf) is widely accepted as the first choice. however, difficulties associated with pcr product size and required technology have encouraged the development of novel typing techniques. in this study, a new genotyping method based on hypervariable tande ...201627701437
prevention of polymicrobial biofilms composed of pseudomonas aeruginosa and pathogenic fungi by essential oils from selected citrus species.mixed microbial infections caused by pseudomonas aeruginosa and pathogenic fungi are commonly found in patients with chronic infections and constitute a significant health care burden. the aim of this study was to address the potential polymicrobial antibiofilm activity of pompia and grapefruit essential oils (eos). the mechanism of antimicrobial activity of eos was analysed. eos of pompia and grapefruit inhibited fungal growth with mic concentrations between 50 and 250 mg l(-1), whereas no effe ...201627702795
experimental induction of mycotic plaques in the guttural pouches of horses.guttural pouch mycosis (gpm) is a rare but potentially life-threatening condition in horses. gpm is caused by a fungal invasion into the mucosal lining of the guttural pouches and, frequently, the associated neurovascular structures. although several species of fungi have been associated with this disease, aspergillus spp. appear to be the most common isolated from the guttural pouches. however, it remains unclear which are the predisposing factors leading to the development of the infection. th ...201627703018
aspergillus fumigatus in the cystic fibrosis lung: pros and cons of azole therapy.aspergillus fumigatus is the main fungus cultured in the airways of patients with cystic fibrosis (cf). allergic bronchopulmonary aspergillosis occurs in ~10% of cf patients and is clearly associated with airway damage and lung function decline. the effects of a. fumigatus colonization in the absence of allergic bronchopulmonary aspergillosis are less well established. retrospective clinical studies found associations of a. fumigatus-positive cultures with computed tomography scan abnormalities, ...201627703383
antifungal long-chain alkenyl sulphates isolated from culture broths of the fungus chaetopsina sp.during a high-throughput screening program focused on the discovery and characterization of new antifungal compounds, a total of 8320 extracts from fundacion medina's collection were screened against a panel of 6 fungal parasitic strains, namely candida glabrata, candida krusei, candida parapsilosis, candida tropicalis, candida albicans, and aspergillus fumigatus. a total of 127 extracts displayed antifungal properties and, after lc/ms dereplication, 10 were selected for further fractionation. b ...201627706529
genome-wide transcriptome analysis of aspergillus fumigatus exposed to osmotic stress reveals regulators of osmotic and cell wall stresses that are saka(hog1) and mpkc dependent.invasive aspergillosis is predominantly caused by aspergillus fumigatus, and adaptations to stresses experienced within the human host are a prerequisite for the survival and virulence strategies of the pathogen. the central signal transduction pathway operating during hyperosmotic stress is the high osmolarity glycerol mitogen-activated protein kinase cascade. a. fumigatus mpkc and saka, orthologues of the saccharomyces cerevisiae hog1p, constitute the primary regulator of the hyperosmotic stre ...201727706915
epidemiological and genomic landscape of azole resistance mechanisms in aspergillus fungi.invasive aspergillosis is a life-threatening mycosis caused by the pathogenic fungus aspergillus. the predominant causal species is aspergillus fumigatus, and azole drugs are the treatment of choice. azole drugs approved for clinical use include itraconazole, voriconazole, posaconazole, and the recently added isavuconazole. however, epidemiological research has indicated that the prevalence of azole-resistant a. fumigatus isolates has increased significantly over the last decade. what is worse i ...201627708619
genetic susceptibility to allergic bronchopulmonary aspergillosis in asthma: a genetic association study.in patients with asthma, the fungus aspergillus fumigatus can cause allergic bronchopulmonary aspergillosis (abpa). familial abpa is reported, and some genetic factors have been associated with the disease, however, these are small studies (n ≤ 38) and do not explain all cases of abpa.201627708669
agents that activate the high osmolarity glycerol pathway as a means to combat pathogenic molds.treatment of invasive fungal infections often fails due to the limited number of therapeutic options. in this study, we have analyzed the impact of agents activating the high osmolarity glycerol (hog) pathway on molds that cause infections in humans and livestock. we found that agents like fludioxonil and iprodione, have a clear anti-fungal activity against pathogenic aspergillus, lichtheimia, rhizopus and scedosporium species. only a. terreus turned out to be resistant to fludioxonil, even thou ...201627713026
case series of omalizumab for allergic bronchopulmonary aspergillosis in cystic fibrosis patients.allergic bronchopulmonary aspergillosis (abpa) affects up to 15% of patients with cystic fibrosis (cf). corticosteroids are used as first-line therapy, but relapse and adverse effects commonly occur. case reports have suggested the efficacy of the anti-ige recombinant humanized monoclonal antibody omalizumab. a retrospective multicenter observational french study retrieved 32 cf patients (11 children and 21 adults) who have received omalizumab for more than 3 months in the context of abpa. clini ...201727717223
hyperspectral imaging using intracellular spies: quantitative real-time measurement of intracellular parameters in vivo during interaction of the pathogenic fungus aspergillus fumigatus with human monocytes.hyperspectral imaging (hsi) is a technique based on the combination of classical spectroscopy and conventional digital image processing. it is also well suited for the biological assays and quantitative real-time analysis since it provides spectral and spatial data of samples. the method grants detailed information about a sample by recording the entire spectrum in each pixel of the whole image. we applied hsi to quantify the constituent ph variation in a single infected apoptotic monocyte as a ...201627727286
short interspersed nuclear element (sine) sequences in the genome of the human pathogenic fungus aspergillus fumigatus af293.novel families of short interspersed nuclear element (sine) sequences in the human pathogenic fungus aspergillus fumigatus, clinical isolate af293, were identified and categorised into trna-related and 5s rrna-related sines. eight predicted trna-related sine families originating from different trnas, and nominated as afusine2 sequences, contained target site duplications of short direct repeat sequences (4-14 bp) flanking the elements, an extended trna-unrelated region and typical features of rn ...201627736869
aspergillus fumigatus responds to natural killer (nk) cells with upregulation of stress related genes and inhibits the immunoregulatory function of nk cells.natural killer (nk) cells are active against aspergillus fumigatus, which in turn is able to impair the host defense. unfortunately, little is known on the mutual interaction of nk cells and a. fumigatus. we coincubated human nk cells with a. fumigatus hyphae and assessed the gene expression and protein concentration of selected molecules. we found that a. fumigatus up-regulates the gene expression of pro-inflammatory molecules in nk cells, but inhibited the release of these molecules resulting ...201627738337
predicting aspergillus fumigatus exposure from composting facilities using a dispersion model: a conditional calibration and validation.bioaerosols are released in elevated quantities from composting facilities and are associated with negative health effects, although dose-response relationships are unclear. exposure levels are difficult to quantify as established sampling methods are costly, time-consuming and current data provide limited temporal and spatial information. confidence in dispersion model outputs in this context would be advantageous to provide a more detailed exposure assessment. we present the calibration and va ...201727745825
ergothioneine biosynthesis and functionality in the opportunistic fungal pathogen, aspergillus fumigatus.ergothioneine (egt; 2-mercaptohistidine trimethylbetaine) is a trimethylated and sulphurised histidine derivative which exhibits antioxidant properties. here we report that deletion of aspergillus fumigatus egta (afua_2g15650), which encodes a trimodular enzyme, abrogated egt biosynthesis in this opportunistic pathogen. egt biosynthetic deficiency in a. fumigatus significantly reduced resistance to elevated h2o2 and menadione, respectively, impaired gliotoxin production and resulted in attenuate ...201627748436
1,25(oh)2d3 and vdr signaling pathways regulate the inhibition of dectin-1 caused by cyclosporine a in response to aspergillus fumigatus in human corneal epithelial cells.the objective of this study is to observe whether cyclosporine a (csa) inhibits the expression of dectin-1 in human corneal epithelial cells infected with aspergillus fumigatus (a. fumigatus) and to investigate the molecular mechanisms of the inhibition.201627755569
analysis and description of the stages of aspergillus fumigatus biofilm formation using scanning electron microscopy.biofilms are a highly structured consortia of microorganisms that adhere to a substrate and are encased within an extracellular matrix (ecm) that is produced by the organisms themselves. aspergillus fumigatus is a biotechnological fungus that has a medical and phytopathogenic significance, and its biofilm occurs in both natural and artificial environments; therefore, studies on the stages observed in biofilm formation are of great significance due to the limited knowledge that exists on this spe ...201627756222
the use of pyranoside-into-furanoside rearrangement and controlled o(5) → o(6) benzoyl migration as the basis of a synthetic strategy to assemble (1→5)- and (1→6)-linked galactofuranosyl chains.a new pyranoside-into-furanoside (pif) rearrangement of selectively protected galactopyranosides, followed by controlled o(5) → o(6) benzoate migration, gives either 5-oh or 6-oh products. it has been applied for the synthesis of four oligosaccharides related to the galactomannan from aspergillus fumigatus. the assembly of target oligosaccharides containing both (1→5) and (1→6) linkages between galactofuranosyl residues was performed by applying terminal mannoside and digalactofuranoside blocks, ...201627759393
synergistic effect of anidulafungin combined with posaconazole in experimental aspergillosis.clinical and experimental data have shown discrepancies on the efficacy of combinations between triazoles and echinocandins. in this study, anidulafungin plus posaconazole have shown efficacy against a murine systemic infection by three strains of aspergillus fumigatus the combination increased mice survival and reduced burden in the kidneys over the corresponding monotherapies and voriconazole. clearance of kidneys was observed in 62% to 100% of animals (strain dependant). we observed good in v ...201627760829
fungal sex: the ascomycota.this article provides an overview of sexual reproduction in the ascomycetes, a phylum of fungi that is named after the specialized sacs or "asci" that hold the sexual spores. they have therefore also been referred to as the sac fungi due to these characteristic structures that typically contain four to eight ascospores. ascomycetes are morphologically diverse and include single-celled yeasts, filamentous fungi, and more complex cup fungi. the sexual cycles of many species, including those of the ...201627763253
antifungal drugs: the current armamentarium and development of new agents.invasive fungal infections are becoming an increasingly important cause of human mortality and morbidity, particularly for immunocompromised populations. the fungal pathogens candida albicans, cryptococcus neoformans, and aspergillus fumigatus collectively contribute to over 1 million human deaths annually. hence, the importance of safe and effective antifungal therapeutics for the practice of modern medicine has never been greater. given that fungi are eukaryotes like their human host, the numb ...201627763259
mdn-0170, a new napyradiomycin from streptomyces sp. strain ca-271078.a new napyradiomycin, mdn-0170 (1), was isolated from the culture broth of the marine-derived actinomycete strain ca-271078, together with three known related compounds identified as 4-dehydro-4a-dechloronapyradiomycin a1 (2), napyradiomycin a1 (3) and 3-chloro-6,8-dihydroxy-8-α-lapachone (4). the structure of the new compound was determined using a combination of spectroscopic techniques, including 1d and 2d nmr and electrospray-time of flight mass spectrometry (esi-tof ms). the relative config ...201627763545
regulatory t cell specificity directs tolerance versus allergy against aeroantigens in humans.foxp3+ regulatory t cells (tregs) maintain tolerance against self-antigens and innocuous environmental antigens. however, it is still unknown whether treg-mediated tolerance is antigen specific and how treg specificity contributes to the selective loss of tolerance, as observed in human immunopathologies such as allergies. here, we used antigen-reactive t cell enrichment to identify antigen-specific human tregs. we demonstrate dominant treg-mediated tolerance against particulate aeroallergens, s ...201627773482
extrapulmonary aspergillus infection in patients with card9 deficiency.invasive pulmonary aspergillosis is a life-threatening mycosis that only affects patients with immunosuppression, chemotherapy-induced neutropenia, transplantation, or congenital immunodeficiency. we studied the clinical, genetic, histological, and immunological features of 2 unrelated patients without known immunodeficiency who developed extrapulmonary invasive aspergillosis at the ages of 8 and 18. one patient died at age 12 with progressive intra-abdominal aspergillosis. the other patient had ...201627777981
differential regulation of myeloid-derived suppressor cells by candida species.myeloid-derived suppressor cells (mdscs) are innate immune cells characterized by their ability to suppress t-cell responses. recently, we demonstrated that the human-pathogenic fungi candida albicans and aspergillus fumigatus induced a distinct subset of neutrophilic mdscs. to dissect candida-mediated mdsc induction in more depth, we studied the relative efficacy of different pathogenic non-albicans candida species to induce and functionally modulate neutrophilic mdscs, including c. glabrata, c ...201627790210
efficacy of aqueous and methanolic extracts of rheum spiciformis against pathogenic bacterial and fungal strains.rheum spiciformis is a newly identified edible medicinal plant of genus rheum. the plant is used to treat various diseases on traditional levels in kashmir valley, india.201627790424
f901318 represents a novel class of antifungal drug that inhibits dihydroorotate dehydrogenase.there is an important medical need for new antifungal agents with novel mechanisms of action to treat the increasing number of patients with life-threatening systemic fungal disease and to overcome the growing problem of resistance to current therapies. f901318, the leading representative of a novel class of drug, the orotomides, is an antifungal drug in clinical development that demonstrates excellent potency against a broad range of dimorphic and filamentous fungi. in vitro susceptibility test ...201627791100
spectrophotometric reading of eucast antifungal susceptibility testing of aspergillus fumigatus.given the increasing number of antifungal drugs and the emergence of resistant aspergillus isolates, objective, automated and high-throughput antifungal susceptibility testing is important. the eucast e.def 9.3 reference method for mic determination of aspergillus species relies on visual reading. spectrophotometric reading was not adopted because of concern that non-uniform filamentous growth might lead to unreliable and non-reproducible results. we therefore evaluated spectrophotometric readin ...201727793736
airway epithelial kif3a regulates th2 responses to aeroallergens.kif3a, the gene encoding kinesin family member 3a, is a susceptibility gene locus associated with asthma; however, mechanisms by which kif3a might influence the pathogenesis of the disorder are unknown. in this study, we deleted the mouse kif3a gene in airway epithelial cells. both homozygous and heterozygous kif3a gene-deleted mice were highly susceptible to aeroallergens from aspergillus fumigatus and the house dust mite, resulting in an asthma-like pathology characterized by increased goblet ...201627794000
prospective biomarker screening for diagnosis of invasive aspergillosis in high-risk pediatric patients.combined biomarker screening is increasingly used to diagnose invasive aspergillosis (ia) in high-risk patients. in adults, the combination of galactomannan (gm) and fungal dna detection has proven to be beneficial in the diagnosis of ia. data in purely pediatric cohorts are scarce. here, we monitored 39 children shortly before and after allogeneic stem cell transplantation twice weekly by use of a commercial gm enzyme-linked immunosorbent assay (elisa) and a pcr assay based on amplification of ...201727795339
genotyping of aspergillus fumigatus reveals compartmentalization of genotypes in disseminated disease after invasive pulmonary aspergillosis. 201727795351
multicenter study of method-dependent epidemiological cutoff values for detection of resistance in candida spp. and aspergillus spp. to amphotericin b and echinocandins for the etest agar diffusion method.method-dependent etest epidemiological cutoff values (ecvs) are not available for susceptibility testing of either candida or aspergillus species with amphotericin b or echinocandins. in addition, reference caspofungin mics for candida spp. are unreliable. candida and aspergillus species wild-type (wt) etest mic distributions (microorganisms in a species-drug combination with no detectable phenotypic resistance) were established for 4,341 candida albicans, 113 c. dubliniensis, 1,683 c. glabrata ...201727799206
screening and characterization of a non-cyp51a mutation in an aspergillus fumigatus cox10 strain conferring azole resistance.the rapid and global emergence of azole resistance in the human pathogen aspergillus fumigatus has drawn attention. thus, a thorough understanding of its mechanisms of drug resistance requires extensive exploration. in this study, we found that the loss of the putative calcium-dependent protein-encoding gene alga causes an increased frequency of azole-resistant a. fumigatus isolates. in contrast to previously identified azole-resistant isolates related to cyp51a mutations, only one isolate carri ...201727799210
evaluation of mic strip isavuconazole test for susceptibility testing of wild-type and non-wild-type aspergillus fumigatus isolates.we evaluated the mic strip isavuconazole test against eucast e.def 9.3 by using 40 wild-type and 39 cyp51a mutant aspergillus fumigatus strains. the strip full inhibition endpoint (fie) and 80% growth inhibition endpoint were determined by two independent readers, reader 1 (r1) and r2. the essential (within ±0, ±1, and ±2 twofold dilutions) and categorical agreements were best with the fie (for r1/r2, 42%/41%, 75%/73%, and 90%/89% for essential agreement, and 91.1%/92.4% categorical agreement, w ...201727799223
posaconazole-loaded leukocytes as a novel treatment strategy targeting invasive pulmonary aspergillosis.impaired delivery of antifungals to hyphae within necrotic lesions is thought to contribute to therapeutic failure in invasive pulmonary aspergillosis (ipa). we hypothesized that transfusion of leukocytes loaded ex vivo with the lipophilic antifungal posaconazole could improve delivery of antifungals to the sites of established infection and improve outcome in experimental ipa.201627799353
deletion of afpab1 causes increased sensitivity to oxidative stress and hypovirulence in aspergillus fumigatus.aspergillus fumigatus afpab1 is the ortholog of the aspergillus oryzae cytoplasmic messenger ribonucleoprotein granules aopab1 that function to depress the initiation of translation during stress. a. fumigatus can regulate its cellular physiology in response to environmental stresses, but there has been no research on pab1 in a. fumigatus. the associated gene afpab1 was replaced with a hygromycin-selectable marker to generate the strain δafpab1. phenotypic analysis showed that the δafpab1 grew m ...201627801871
Refresh
firstprev
...919293949596
nextlast
Displaying items 9101 - 9200 of 9580