Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| floral-dip transformation of arabidopsis thaliana to examine ptso2::beta-glucuronidase reporter gene expression. | the ability to introduce foreign genes into an organism is the foundation for modern biology and biotechnology. in the model flowering plant arabidopsis thaliana, the floral-dip transformation method has replaced all previous methods because of its simplicity, efficiency, and low cost. specifically, shoots of young flowering arabidopsis plants are dipped in a solution of agrobacterium tumefaciens carrying specific plasmid constructs. after dipping, the plants are returned to normal growth and yi ... | 2010 | 20548285 |
| stable integration and expression of wasabi defensin gene in "egusi" melon (colocynthis citrullus l.) confers resistance to fusarium wilt and alternaria leaf spot. | production of "egusi" melon (colocynthis citrullus l.) in west africa is limited by fungal diseases, such as alternaria leaf spot and fusarium wilt. in order to engineer "egusi" resistant to these diseases, cotyledonary explants of two "egusi" genotypes, 'ejagham' and nhc1-130, were transformed with agrobacterium tumefaciens strain eha101 harbouring wasabi defensin gene (isolated from wasabia japonica l.) in a binary vector pekh1. after co-cultivation for 3 days, infected explants were transferr ... | 2010 | 20552202 |
| plant-expressed recombinant mountain cedar allergen jun a 1 is allergenic and has limited pectate lyase activity. | mountain cedar (juniperus ashei) pollen commonly causes a winter time allergic rhinitis in the central usa. jun a 1 is the dominant allergenic protein, but biologically active recombinant jun a 1 has not been successfully expressed, despite numerous attempts with several expression systems. | 2010 | 20559000 |
| agrobacterium-mediated t-dna transfer and integration into the chromosome of streptomyces lividans. | summary agrobacterium tumefaciens is the prototype of a prokaryotic organism transmitting dna across natural kingdom barriers into higher cells. in nature, a specific segment (t-dna) of the resident ti plasmid is transferred from this bacterium into plant cells and integrated into the plant cell genome. expression of the integrated oncogenes contained in the t-dna results in the tumour disease known as crown gall. besides plants, the range of transformable recipients is broad and includes fungi ... | 2002 | 20569318 |
| characterization of oncogene-silenced transgenic plants: implications for agrobacterium biology and post-transcriptional gene silencing. | summary agrobacterium tumefaciens tumorigenesis is initiated by the horizontal transfer of a suite of oncogenes that alter hormone synthesis and sensitivity in infected plant cells. transgenic plants silenced for the iaam and ipt oncogenes are highly recalcitrant to tumorigenesis, and present a unique resource to elucidate fundamental questions related to agrobacterium biology and post-transcriptional gene silencing (ptgs). the oncogene-silenced transgenic tomato line 01/6 was used to characteri ... | 2003 | 20569363 |
| agrobacterium tumefaciens mediated transformation of the oomycete plant pathogen phytophthora infestans. | summary agrobacterium tumefaciens is widely used for plant dna transformation and, more recently, has also been used to transform yeast and filamentous fungi. here we present a protocol for agrobacterium-mediated dna transformation of the oomycete phytophthora infestans, the causal agent of potato late blight. binary t-dna vectors containing neomycin phosphotransferase (npt) and beta-glucuronidase (gus) fused to oomycete transcriptional regulatory sequences were constructed. seven days of co-cul ... | 2003 | 20569405 |
| backbone-free transformation of barrel medic (medicago truncatula) with a medicago-derived transfer dna. | in the present work, agrobacterium tumefaciens-mediated genetic transformation of the model legume medicago truncatula gaertn. (barrel medic) was carried out using the psim843 vector that contains a medicago-derived transfer dna, delineated by a 25-bp sequence homologous to bacterial t-dna borders. the transfer dna contains an expression cassette for the nptii (neomycin phosphotransferase) gene and is flanked by an expression cassette for the backbone integration marker gene ipt (isopentenyl tra ... | 2010 | 20571798 |
| from host recognition to t-dna integration: the function of bacterial and plant genes in the agrobacterium-plant cell interaction. | abstract agrobacterium tumefaciens and its related species, a. rhizogenes and a. vitis, are the only known bacterial pathogens which 'genetically invade' host plants and stably integrate part of their genetic material into the host cell genome. thus, a. tumefaciens has evolved as a major tool for plant genetic engineering. furthermore, this unique process of interkingdom dna transfer has been utilized as a model system for studies of its underlying biological events, such as intercellular signal ... | 2000 | 20572967 |
| nematodes as vectors to introduce agrobacterium into plant roots. | summary a fast plant promoter test was developed by means of a nematode to transfer agrobacterium tumefaciens into plant roots. two-week-old arabidopsis thaliana (l.) heynh. plants were transferred to infection medium. meloidogyne incognita or heterodera schachtii juveniles were mixed with the agrobacterium strain that harboured the binary vector, and this mixture was used for plant inoculation. during migration of the nematode and establishment of the feeding site inside the roots, the t-dna wa ... | 2000 | 20572986 |
| type iii secretion and in planta recognition of the xanthomonas avirulence proteins avrbs1 and avrbst. | summary the hrp gene cluster of the plant pathogen xanthomonas campestris pv. vesicatoria (xcv) encodes a type iii secretion system required for the delivery of virulence and avirulence proteins into the plant. some of these effector proteins, e.g. avrbs1 and avrbst, are recognized by pepper plants carrying corresponding resistance genes, triggering the hypersensitive reaction (hr). in this study, epitope tagged avrbs1 and avrbst proteins were detected in culture supernatants only in the presenc ... | 2001 | 20573017 |
| agrobacterium tumefaciens exor represses succinoglycan biosynthesis and is required for biofilm formation and motility. | the ubiquitous plant pathogen agrobacterium tumefaciens attaches efficiently to plant tissues and abiotic surfaces and can form complex biofilms. a genetic screen for mutants unable to form biofilms on pvc identified disruptions in a homologue of the exor gene. exor is a predicted periplasmic protein, originally identified in sinorhizobium meliloti, but widely conserved among alphaproteobacteria. disruptions in the a. tumefaciens exor gene result in severely compromised attachment to abiotic sur ... | 2010 | 20576688 |
| gene transfer into solanum tuberosum via rhizobium spp. | agrobacterium tumefaciens-mediated transformation (atmt) is the preferred technique for gene transfer into crops. a major disadvantage of the technology remains the complexity of the patent landscape that surrounds atmt which restricts its use for commercial applications. an alternative system has been described (broothaerts et al. in nature 433:629-633, 2005) detailing the propensity of three rhizobia to transform the model crop arabidopsis thaliana, the non-food crop nicotiana tabacum and, at ... | 2011 | 20582626 |
| rhizobium rosettiformans sp. nov., isolated from a hexachlorocyclohexane dump site, and reclassification of blastobacter aggregatus hirsch and muller 1986 as rhizobium aggregatum comb. nov. | a gram-negative, rod-shaped, motile, aerobic bacterial strain, w3(t), was isolated from hexachlorocyclohexane (hch)-contaminated groundwater from lucknow, india, and its taxonomic position was determined using a polyphasic approach. strain w3(t) shared highest 16s rrna gene sequence similarity of 97.8 % with rhizobium selenitireducens b1(t), followed by rhizobium daejeonense l61(t) (97.7 %), rhizobium radiobacter atcc 19358(t) (97.5 %) and blastobacter aggregatus ifam 1003(t) (97.2 %). strain w3 ... | 2011 | 20584817 |
| a fine control of quorum-sensing communication in agrobacterium tumefaciens. | the bacterial pathogen agrobacterium tumefaciens produces the quorum-sensing (qs) signal 3-oxo-octanoylhomoserine lactone (oc8hsl) for controlling horizontal transfer of its tumor inducing (ti) plasmid that carries both the t-dna and the virulence genes. over-accumulation of oc8hsl also increases severity of plant symptoms (number of emerging tumors at infection site) by an unknown mechanism. a. tumefaciens strain c58 expresses two lactonases, attm (blcc) and aiib, that cleave oc8hsl and are pot ... | 2010 | 20585496 |
| pharmacokinetics of opda, an organophosphorus hydrolase, in the african green monkey. | organophosphorus (op) pesticides are a broad class of acetylcholinesterase inhibitors that are responsible for tremendous morbidity and mortality worldwide, contributing to an estimated 300,000 deaths annually. current pharmacotherapy for acute op poisoning includes the use of atropine, an oxime, and benzodiazepines. however, even with such therapy, the mortality from these agents are as high as 40%. enzymatic hydrolysis of ops is an attractive new potential therapy for acute op poisoning. a num ... | 2010 | 20599794 |
| activation of an arabidopsis resistance protein is specified by the in planta association of its leucine-rich repeat domain with the cognate oomycete effector. | activation of plant immunity relies on recognition of pathogen effectors by several classes of plant resistance proteins. to discover the underlying molecular mechanisms of effector recognition by the arabidopsis thaliana recognition of peronospora parasitica1 (rpp1) resistance protein, we adopted an agrobacterium tumefaciens-mediated transient protein expression system in tobacco (nicotiana tabacum), which allowed us to perform coimmunoprecipitation experiments and mutational analyses. herein, ... | 2010 | 20601497 |
| analysis of core genes supports the reclassification of strains agrobacterium radiobacter k84 and agrobacterium tumefaciens ake10 into the species rhizobium rhizogenes. | some strains of the former genus agrobacterium have high biotechnological interest and are currently misclassified. consequently, in this study, the taxonomic status of the non-pathogenic strain agrobacterium radiobacter k84, used in biological control, and the tumourigenic strain agrobacterium tumefaciens ake10, able to regenerate tobacco transgenic plants, was revised. the phylogenetic analysis of the chromosomal genes rrs, atpd and reca showed that they should be reclassified into rhizobium r ... | 2010 | 20627641 |
| a conserved mechanism of gaba binding and antagonism is revealed by structure-function analysis of the periplasmic binding protein atu2422 in agrobacterium tumefaciens. | bacterial periplasmic binding proteins (pbps) and eukaryotic pbp-like domains (also called as venus flytrap modules) of g-protein-coupled receptors are involved in extracellular gaba perception. we investigated the structural and functional basis of ligand specificity of the pbp atu2422, which is implicated in virulence and transport of gaba in the plant pathogen agrobacterium tumefaciens. five high-resolution x-ray structures of atu2422 liganded to gaba, pro, ala, and val and of point mutant at ... | 2010 | 20630861 |
| effect of codon optimization and subcellular targeting on toxoplasma gondii antigen sag1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice. | codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the sag178-322 antigen of toxoplasma gondii in tobacco leaves. the expression of the tobacco-optimized and native versions of the sag1 gene was explored by transient expression from the agrobacterium tumefaciens binary expression vector, which allows targeting the recombinant protein to the endoplasmic reticulum (er) and the apoplast. finally, mice were subcutaneously and orally immunized ... | 2010 | 20633272 |
| acc deaminase increases the agrobacterium tumefaciens-mediated transformation frequency of commercial canola cultivars. | the plant hormone ethylene has been reported to inhibit the agrobacterium tumefaciens-mediated transformation efficiency of many plants. in this study, an acds gene that encodes 1-aminocyclopropane-1-carboxylic acid (acc) deaminase, an enzyme that breaks down acc, the direct precursor of ethylene biosynthesis in all higher plants, was introduced into a. tumefaciens gv3101::pmp90. it was found that the presence of active acc deaminase in a. tumefaciens reduced ethylene levels produced by plant ti ... | 2010 | 20636976 |
| o-methyltransferase(s)-suppressed plants produce lower amounts of phenolic vir inducers and are less susceptible to agrobacterium tumefaciens infection. | the first step of agrobacterium tumefaciens/plant interaction corresponds to the activation of a transduction pathway of the bacterium by plant exudate. phenolic compounds rapidly secreted by wounded plant cells induce the expression of bacterial virulence (vir) genes; however, little is known about their biosynthesis in plant. here we show that inoculation of an agrobacterium tumefaciens virulent strain on orthodiphenol-o-methyltransferases-suppressed tobacco plants leads to significantly small ... | 2010 | 20652308 |
| phosphatidylcholine biosynthesis and its significance in bacteria interacting with eukaryotic cells. | phosphatidylcholine (pc), a typical eukaryotic membrane phospholipid, is present in only about 10% of all bacterial species, in particular in bacteria interacting with eukaryotes. a number of studies revealed that pc plays a fundamental role in symbiotic and pathogenic microbe-host interactions. agrobacterium tumefaciens mutants lacking pc are unable to elicit plant tumors. the human pathogens brucella abortus and legionella pneumophila require pc for full virulence. the plant symbionts bradyrhi ... | 2010 | 20656373 |
| homologs of aminoacyl-trna synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis. | aminoacyl-trna synthetases (aarss) are ancient and evolutionary conserved enzymes catalyzing the formation of aminoacyl-trnas, that are used as substrates for ribosomal protein biosynthesis. in addition to full length aars genes, genomes of many organisms are sprinkled with truncated genes encoding single-domain aars-like proteins, which often have relinquished their canonical role in genetic code translation. we have identified the genes for putative seryl-trna synthetase homologs widespread in ... | 2010 | 20663952 |
| bathy phytochromes in rhizobial soil bacteria. | phytochromes are biliprotein photoreceptors that are found in plants, bacteria, and fungi. prototypical phytochromes have a pr ground state that absorbs in the red spectral range and is converted by light into the pfr form, which absorbs longer-wavelength, far-red light. recently, some bacterial phytochromes have been described that undergo dark conversion of pr to pfr and thus have a pfr ground state. we show here that such so-called bathy phytochromes are widely distributed among bacteria that ... | 2010 | 20675484 |
| urate is a ligand for the transcriptional regulator pecs. | pecs is a member of the marr (multiple antibiotic resistance regulator) family, which has been shown in erwinia to regulate the expression of virulence genes. marr homologs typically bind a small molecule ligand, resulting in attenuated dna binding. for pecs, the natural ligand has not been identified. we have previously shown that urate is a ligand for the deinococcus radiodurans-encoded marr homolog hucr (hypothetical uricase regulator) and identified residues responsible for ligand binding. w ... | 2010 | 20678501 |
| [transformation of enhanced green fluorescent protein gene in paecilomyces lilacinus mediated by agrobacterium tumefaciens]. | the main aim of this study was to transform the enhanced green fluorescent protein gene (egfp) into biocontrol fungus paecilomyces lilacinus strain 9410. we constructed the expression vector pupngt of the fusion gene nptii-egfp using pcdna3.1(-) as a helper plasmid. the egfp gene was then transformed into p. lilacinus strain 9410 via agrobacterium tumefaciens-mediated transformation. pcr and southern blotting analysis showed that the egfp gene was integrated into the genomes of the tested transf ... | 2010 | 20684307 |
| cholera toxin b subunit-domain iii of dengue virus envelope glycoprotein e fusion protein production in transgenic plants. | envelope glycoprotein e of the dengue virus, which plays a crucial role in its entry into host cells, has an immunogenic domain iii (eiii, amino acids 297-394), which is capable of inducing neutralizing antibodies. however, mice immunized with eiii protein without adjuvant elicited low immune responses. to improve low immune responses, a dna fragment, consisting of cholera toxin b subunit and eiii gene (ctb-eiii), was constructed and introduced into tobacco plant cells (nicotiana tabacum l. cv. ... | 2010 | 20691270 |
| agrobacterium-produced and exogenous cytokinin-modulated agrobacterium-mediated plant transformation. | agrobacterium tumefaciens is a plant pathogenic bacterium that causes neoplastic growths, called 'crown gall', via the transfer and integration of transferred dna (t-dna) from the bacterium into the plant genome. we characterized an acetosyringone (as)-induced tumour-inducing (ti) plasmid gene, tzs (trans-zeatin synthesizing), that is responsible for the synthesis of the plant hormone cytokinin in nopaline-type a. tumefaciens strains. the loss of tzs protein expression and trans-zeatin secretion ... | 2010 | 20696005 |
| new capabilities for mycosphaerella graminicola research. | mycosphaerella graminicola is a major pathogen of wheat worldwide, causing septoria leaf blotch disease. targeted gene disruption in m. graminicola, by agrobacterium tumefaciens-mediated transformation, has become an established functional genomics tool for m. graminicola research in recent years. however, in order to advance research into this economically important pathogen, further functional genomics tools need to be developed. here, we report three new capabilities for m. graminicola resear ... | 2010 | 20696006 |
| development of efficient plant regeneration and transformation system for impatiens using agrobacterium tumefaciens and multiple bud cultures as explants. | impatiens (impatiens walleriana) is a top selling floriculture crop. the potential for genetic transformation of impatiens to introduce novel flower colors or virus resistance has been limited by its general recalcitrance to tissue culture and transformation manipulations. we have established a regeneration and transformation system for impatiens that provides new alternatives to genetic improvement of this crop. | 2010 | 20696066 |
| accumulation of copper in trichoderma reesei transformants, constructed with the modified agrobacterium tumefaciens-mediated transformation technique. | a modified agrobacterium tumefaciens-mediated transformation method was established for the construction of mutants with improved copper tolerance and accumulation capability in trichoderma reesei. one transformant, at01, exhibited the highest copper accumulation capability. with copper at 0.7 mm, at01 removed 13 mg copper/g biomass (removal rate of 96%), whereas the wild-type strain removed only 6 mg copper/g biomass (removal rate of 50%). optimal conditions were a ph of 5.0 at 28°c. the pigmen ... | 2010 | 20697931 |
| mass spectrometric analysis reveals remnants of host-pathogen molecular interactions at the starch granule surface in wheat endosperm. | the starch granules of wheat seed are solar energy-driven deposits of fixed carbon and, as such, present themselves as targets of pathogen attack. the seed's array of antimicrobial proteins, peptides, and small molecules comprises a molecular defense against penetrating pathogens. in turn, pathogens exhibit an arsenal of enzymes to facilitate the degradation of the host's endosperm. in this context, the starch granule surface is a relatively unexplored domain in which unique molecular barriers m ... | 2010 | 20701481 |
| promotion of monacolin k production by agrobacterium tumefaciens-mediated transformation in monascus albidus 9901. | the binary vector pcambia3300-gpda-hph-trpc with hygromycin b phosphotransferase (hph) was constructed and transformed into monascus albidus 9901 by agrobacterium tumefaciens-mediated transformation, with gene hph as the selective marker. in order to improve the efficiency of a. tumefaciens-mediated transformation in m. albidus 9901, we optimized various factors including concentration of m. albidus 9901 spores, cell density of a. tumefaciens, co-cultivation time, temperature, and acetosyringone ... | 2011 | 20717674 |
| component identification of electron transport chains in curdlan-producing agrobacterium sp. atcc 31749 and its genome-specific prediction using comparative genome and phylogenetic trees analysis. | agrobacterium sp. atcc 31749 (formerly named alcaligenes faecalis var. myxogenes) is a non-pathogenic aerobic soil bacterium used in large scale biotechnological production of curdlan. however, little is known about its genomic information. dna partial sequence of electron transport chains (etcs) protein genes were obtained in order to understand the components of etc and genomic-specificity in agrobacterium sp. atcc 31749. degenerate primers were designed according to etc conserved sequences in ... | 2010 | 20730594 |
| [cloning of keratinocyte growth factor 2 gene (kgf2) and its transformation to brassica napus l]. | recently, more research about the plant bioreactor expressing genes encoding human proteins was reported. in the present study, the cdna of the human gene keratinocyte growth factor 2 (kgf2) was replaced with plant preferred codons by pcr, and the modified full-length cdna was cloned into the plant expression vector pcambia-yo containing the oil-body promoter. the fusion construct pcambia-yo-kgf2 was transformed into brassica napus by agrobacterium tumefacien-mediated cotyledon transformation me ... | 2010 | 20815256 |
| [transformation of didymella bryoniae mediated by agrobacterium tumefaciens]. | gummy stem blight, a plant disease caused by didymella bryoniae, is one of the major diseases in melon. the disease can seriously reduce melon yield and quality. however, little information is available on the genetics and functional genomics of the fungal pathogen. in this study, we developed an agrobacterium-mediated transformation system for d. bryoniae by using a universal pathogenic isolate db11 and the agrobacterium tumefaciens strain c58c1 carrying plasmid pbig2rhph2 harboring the hygromy ... | 2010 | 20815261 |
| asymptomatic catheter related rhizobium radiobacter infection in a haploidentical hemapoetic stem cell recipient. | catheter related infections are reported as one of the most common source of nosocomial infections. rhizobium radibacter infections are generally manifested by fever and leukocytosis. here, a 14 months-old girl diagnosed as t (-) b (-) nk (+) severe combined immunodeficiency (scid) is presented. she had received repeated (x3) unconditioned haploidentical hematopoetic stem cell transplantations. during the follow-up, she has been arised an asymptomatic infection with r. radiobacter, which was iso ... | 2010 | 20818107 |
| [establishment of transformation system in mulberry and biosynthesis of quercetin]. | to establish the transformation system of mulberry, and test its ability of quercetin biosynthesis. | 2010 | 20822005 |
| necessary and sufficient conditions for the asymmetric synthesis of chiral amines using ω-aminotransferases. | the half reactions of ω-aminotransferase (ω-at) from vibrio fluvialis js17 (ω-atvf) were carried out using purified pyridoxal 5'-phosphate-enzyme (plp-enz) and pyridoxamine 5'-phosphate-enzyme (pmp-enz) complexes to investigate the relative activities of substrates. in the reaction generating pmp-enz from plp-enz using l-alanine as an amine donor, l-alanine showed about 70% of the initial reaction rate of (s)-α-methylbenzylamine ((s)-α-mba). however, in the subsequent half reaction recycling plp ... | 2011 | 20824676 |
| genetic transformation of lignin degrading fungi facilitated by agrobacterium tumefaciens. | white-rot fungi are primarily the major degraders of lignin, a major obstacle for commercial exploitation of plant byproducts to produce bioethanol and other industrially important products. however, to improve their efficacy for lignin degradation, it has become necessary to genetically modify these organisms using appropriate vectors. agrobacterium tumefaciens, a soil phytopathogenic bacterium, generally transforms plants by delivering a portion of the resident ti- plasmid, the t-dna (transfer ... | 2010 | 20836896 |
| characterization of liposomes formed by lipopolysaccharides from burkholderia cenocepacia, burkholderia multivorans and agrobacterium tumefaciens: from the molecular structure to the aggregate architecture. | the microstructure of liposomes formed by the lipopolysaccharides (lps) derived from burkholderia cenocepacia et-12 type strain lmg 16656, burkholderia multivorans strain c1576 and agrobacterium tumefaciens strain tt111 has been investigated by a combined experimental strategy, including dynamic light scattering (dls), small-angle neutron scattering (sans) and electron paramagnetic resonance (epr). the results highlight that the lps molecular structure determines, through a complex interplay of ... | 2010 | 20852798 |
| development of an efficient gene targeting system in colletotrichum higginsianum using a non-homologous end-joining mutant and agrobacterium tumefaciens-mediated gene transfer. | the hemibiotrophic ascomycete colletotrichum higginsianum is the casual agent of anthracnose disease of cruciferous plants. high efficiency transformation by agrobacterium tumefaciens-mediated gene transfer has been established for this fungus. however, targeted gene mutagenesis through homologous recombination rarely occurs in c. higginsianum. we have identified and disrupted the c. higginsianum homologue of the human ku70 gene, chku70, which encodes a protein that plays a role in non-homologou ... | 2010 | 20853009 |
| identification of cis- and trans-acting elements in phw126, a representative of a novel group of rolling circle plasmids. | phw126, pigrk, pigms31 and prao1 are the only known members of a novel and as yet uncharacterised family of rolling circle plasmids. phw126 contains only two open reading frames, of which one shows homology to pmv158-family mobilisation proteins. here we provide evidence that the second open reading frame encodes a replication protein (rep). mutation or deletion of this gene resulted in replication deficient constructs, but providing functional rep from a compatible vector rescued these construc ... | 2011 | 20854841 |
| over-expression of pr-10a leads to increased salt and osmotic tolerance in potato cell cultures. | the pr-10a protein (formerly sth-2) is known to be induced by biotic stress in potato. the present study demonstrates that transgenic suspension cells of the potato cultivar desiree over-expressing the pr-10a protein exhibit significantly increased salt and osmotic tolerance compared to the respective wild type cells. a comparison of the proteome pattern of solanum tuberosum suspension cultures cv. desiree before and after the treatment with nacl or sorbitol under equiosmolar conditions (740mos/ ... | 2010 | 20854851 |
| evidence of transfer by conjugation of type iv secretion system genes between bartonella species and rhizobium radiobacter in amoeba. | bartonella species cospeciate with mammals and live within erythrocytes. even in these specific niches, it has been recently suggested by bioinformatic analysis of full genome sequences that lateral gene transfer (lgt) may occur but this has never been demonstrated biologically. here we describe the sequence of the b. rattaustraliani (aust/nh4(t)) circular plasmid (pnh4) that encodes the tra cluster of the type iv secretion system (t4ss) and we eventually provide evidence that bartonella species ... | 2010 | 20856925 |
| [transformation of antimicrobial peptide fusion gene of cecropin b and rabbit np-1 to houttuynia cordata]. | to transform the antimicrobial peptide fusion gene of cecropin b and rabbit np-1(cn) into houttuynia cordata to improve its antimicrobic capability. | 2010 | 20862951 |
| the organophosphate-degrading enzyme from agrobacterium radiobacter displays mechanistic flexibility for catalysis. | the op (organophosphate)-degrading enzyme from agrobacterium radiobacter (opda) is a binuclear metallohydrolase able to degrade highly toxic op pesticides and nerve agents into less or non-toxic compounds. in the present study, the effect of metal ion substitutions and site-directed mutations on the catalytic properties of opda are investigated. the study shows the importance of both the metal ion composition and a hydrogen-bond network that connects the metal ion centre with the substrate-bindi ... | 2010 | 20868365 |
| agrobacterium tumefaciens mediated fused egfp-hph gene expression under the control of gpd promoter in pleurotus ostreatus. | a transformation system for the basidiomycete pleurotus ostreatus was established using agrobacterium-mediated infection. following p. ostreatus glyceraldehyde-3-phosphate dehydrogenase gene analysis, its promoter region including two introns was used as cis-regulatory element to drive expression of enhanced green fluorescent protein (egfp). as a selection marker, the hygromycin phosphotransferase (hph) gene cassette was used in the binary vector ppeh. mycelia without pretreatment were found to ... | 2010 | 20869218 |
| genetic basis for the hierarchical interaction between tobamovirus spp. and l resistance gene alleles from different pepper species. | the pepper l gene conditions the plant's resistance to tobamovirus spp. alleles l(1), l(2), l(3), and l(4) confer a broadening spectra of resistance to different virus pathotypes. in this study, we report the genetic basis for the hierarchical interaction between l genes and tobamovirus pathotypes. we cloned l(3) using map-based methods, and l(1), l(1a), l(1c), l(2), l(2b), and l(4) using a homology-based method. l gene alleles encode coiled-coil, nucleotide-binding, leucine-rich repeat (lrr)-ty ... | 2011 | 20879841 |
| enhanced v-atpase activity contributes to the improved salt tolerance of transgenic tobacco plants overexpressing vacuolar na(+)/h (+) antiporter atnhx1. | atnhx1, a vacuolar na(+)/h(+) antiporter gene from arabidopsis thaliana, was introduced into tobacco genome via agrobacterium tumefaciens-mediated transformation to evaluate the role of vacuolar energy providers in plants salt stress response. compared to the wild-type plants, over-expression of atnhx1 increased salt tolerance in the transgenic tobacco plants, allowing higher germination rates of seeds and successful seedling establishment in the presence of toxic concentrations of nacl. more im ... | 2010 | 20882319 |
| functional characterization of replication and stability factors of an incompatibility group p-1 plasmid from xylella fastidiosa. | xylella fastidiosa strain riv11 harbors a 25-kbp plasmid (pxf-riv11) belonging to the incp-1 incompatibility group. replication and stability factors of pxf-riv11 were identified and used to construct plasmids able to replicate in x. fastidiosa and escherichia coli. replication in x. fastidiosa required a 1.4-kbp region from pxf-riv11 containing a replication initiation gene (trfa) and the adjacent origin of dna replication (oriv). constructs containing trfa and oriv from pveis01, a related incp ... | 2010 | 20935126 |
| agrobacterium tumefaciens mediated transformation of chiv gene to trichoderma harzianum. | as a soil-borne filamentous fungus, trichoderma harzianum exhibits biological control properties because it parasitizes a large variety of phytopathogenic fungi. in this study, the vectors pbi121 and pcambia1301 and cloning vector puc18 were used to successfully construct expression vector pca-gchiv for filamentous fungi transformation mediated by agrobacterium tumefaciens.the chiv gene was successfully transferred into the biocontrol fungus t. harzianum with an efficiency of 90-110 transformant ... | 2010 | 20936373 |
| introduction of large dna inserts into the barley pathogenic fungus, ustilago hordei, via recombined binary bac vectors and agrobacterium-mediated transformation. | genetic transformation of organisms with large genome fragments containing complete genes, with regulatory elements or clusters of genes, can contribute to the functional analysis of such genes. however, large inserts, such as those found on bacterial artificial chromosome (bac) clones, are often not easy to transfer. we exploited an existing technique to convert bac clones, containing genomic dna fragments from the barley-covered smut fungus ustilago hordei to binary bacs (bibacs) to make them ... | 2010 | 20936474 |
| the structure of jann_2411 (duf1470) from jannaschia sp. at 1.45 å resolution reveals a new fold (the abate domain) and suggests its possible role as a transcription regulator. | the crystal structure of jann_2411 from jannaschia sp. strain ccs1, a member of the pfam pf07336 family classified as a domain of unknown function (duf1470), was solved to a resolution of 1.45 å by multiple-wavelength anomalous dispersion (mad). this protein is the first structural representative of the duf1470 pfam family. structural analysis revealed a two-domain organization, with the n-terminal domain presenting a new fold called the abate domain that may bind an as yet unknown ligand. the c ... | 2010 | 20944211 |
| tsukamurella tyrosinosolvens and rhizobium radiobacter sepsis presenting with septic pulmonary emboli. | clin microbiol infect abstract: septic pulmonary embolism (spe) is an uncommon, but life-threatening event that is usually associated with extrapulmonary infections. we report the first case of bilateral spe secondary to a central venous catheter-related bloodstream infection involving pathogens commonly considered environmental contaminants: tsukamurella tyrosinosolvens and rhizobium radiobacter. empirical levofloxacin treatment was confirmed by in vitro susceptibility data and produced prompt ... | 2010 | 20946410 |
| genetic reprogramming of host cells by bacterial pathogens. | during the course of infection, pathogens often induce changes in gene expression in host cells and these changes can be long lasting and global or transient and of limited amplitude. defining how, when, and why bacterial pathogens reprogram host cells represents an exciting challenge that opens up the opportunity to grasp the essence of pathogenesis and its molecular details. | 2009 | 20948607 |
| rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. | most plasmids replicate only within a particular genus or family. | 2010 | 20949038 |
| production of functional recombinant bovine trypsin in transgenic rice cell suspension cultures. | a synthetic bovine trypsinogen (sbtrypsinogen) was synthesized on the basis of rice-optimized codon usage via an overlap pcr strategy, prior to being expressed under the control of the sucrose starvation-inducible rice α-amylase 3d (ramy3d) promoter. secretion of trypsin into the culture medium was achieved by using the existing signal peptide. the plant expression vector was introduced into rice calli (oryza sativa l. cv. dongjin), mediated by agrobacterium tumefaciens. the integration of the s ... | 2010 | 20951807 |
| agrobacterium tumefaciens-mediated transformation for investigation of somatic recombination in the fungal pathogen armillaria mellea. | armillaria root disease is one of the most damaging timber and fruit tree diseases in the world. despite its economic importance, many basic questions about the biology of the causal fungi, armillaria spp., are unanswered. for example, armillaria undergoes matings between diploid and haploid mycelia, which can result in a recombinant diploid without meiosis. evidence of such somatic recombination in natural populations suggests that this reproductive mode may affect the pathogen's ecology. inves ... | 2010 | 20952653 |
| pol ii-directed short rnas suppress the nuclear export of mrna. | the synthesis and subsequent nuclear export of non-coding rna (ncrna) directed by rna polymerase (pol) ii is very sensitive to abiotic and biotic external stimuli including pathogen challenges. to assess whether stress-induced ncrnas may suppress the nuclear export of mrna, we exploited the ability of agrobacterium tumefaciens to co-deliver pol i, ii and iii promoter-based vectors for the transcription of short (s) ncrnas, gfp mrna or genomic rna of plant viruses (tobacco mosaic virus, tmv; or p ... | 2010 | 20953971 |
| upregulation of pyrophosphate: fructose 6-phosphate 1-phosphotransferase (pfp) activity in strawberry. | pyrophosphate: fructose 6-phosphate 1-phosphotransferase (pfp) is a cytosolic enzyme catalyzing the first committed step in glycolysis by reversibly phosphorylating fructose-6-phosphate to fructose-1,6-bisphosphate. the position of pfp in glycolytic and gluconeogenic metabolism, as well as activity patterns in ripening strawberry, suggest that the enzyme may influence carbohydrate allocation to sugars and organic acids. fructose-2,6-bisphosphate activates and tightly regulates pfp activity in pl ... | 2010 | 20960058 |
| diverse rhizobia associated with sophora alopecuroides grown in different regions of loess plateau in china. | a total of seventy-five symbiotic bacterial strains isolated from root nodules of wild sophora alopecuroides grown in different regions of china's loess plateau were characterized. based on the combined rflp patterns, thirty-five genotypes were defined among the rhizobia and they were classified into nine genomic species, including mesorhizobium alhagi and m. gobiense as the main groups, as well as agrobacterium tumefaciens, m. amorphae, phyllobacterium trifolii, rhizobium giardinii, r. indigofe ... | 2010 | 20965680 |
| regeneration of transgenic plants by agrobacterium-mediated transformation of somatic embryos of juvenile and mature quercus robur. | a protocol was developed for genetic transformation of somatic embryos derived from juvenile and mature quercus robur trees. optimal transformation conditions were evaluated on the basis of the results of transient gus expression assays with five oak embryogenic lines and a strain of agrobacterium tumefaciens (eha105) harbouring a p35sgusint plasmid containing a nptii and a uida (gus) genes. for stable transformation, embryo clumps at globular/torpedo stages (4-10 mg) were inoculated with eha105 ... | 2010 | 20972795 |
| bioassays of quorum sensing compounds using agrobacterium tumefaciens and chromobacterium violaceum. | in most bacteria, a global level of regulation exists involving intercellular communication via the production and response to cell density-dependent signal molecules. this cell density-dependent regulation has been termed quorum sensing (qs). qs is a global regulator, which has been associated with a number of important features in bacteria including virulence regulation and biofilm formation. consequently, there is considerable interest in understanding, detecting, and inhibiting qs. acyl homo ... | 2011 | 21031300 |
| presence of opportunistic bacteria (rhizobium spp.) with potential for molecular misdiagnosis among canine and feline clinical samples. | rhizobium radiobacter was detected in 12 of 187 dogs and 2 of 100 cats using a polymerase chain reaction (pcr) assay formerly designed for the rickettsia genus. although pcr primers used for pathogenic infectious agents are specifically assessed to avoid cross-amplification, this retrospective study highlights the importance of sequencing to avoid molecular misdiagnosis. | 2010 | 21037894 |
| a yeast ste11 homologue comekk1 is essential for pathogenesis-related morphogenesis in colletotrichum orbiculare. | several signal transduction pathways, including mitogen-activated protein kinase (mapk) pathways, are involved in appressorium development in colletotrichum orbiculare, the causal agent of cucumber anthracnose disease. in this study, comekk1, a yeast mapk kinases (mapkk) kinase ste11 homolog, was identified as a disrupted gene in an agrobacterium tumefaciens-mediated transformation mutant. the phenotype of comekk1 disruptant was similar to that of cmk1, a saccharomyces cerevisiae fus3/kss1 mapk ... | 2010 | 21039273 |
| enhanced production of single copy backbone-free transgenic plants in multiple crop species using binary vectors with a pri replication origin in agrobacterium tumefaciens. | single transgene copy, vector backbone-free transgenic crop plants are highly desired for functional genomics and many biotechnological applications. we demonstrate that binary vectors that use a replication origin derived from the ri plasmid of agrobacterium rhizogenes (oriri) increase the frequency of single copy, backbone-free transgenic plants in agrobacterium tumefaciens mediated transformation of soybean, canola, and corn, compared to rk2-derived binary vectors (rk2 oriv). in large scale s ... | 2010 | 21042934 |
| transgenic brassica chinensis plants expressing a bacterial coda gene exhibit enhanced tolerance to extreme temperature and high salinity. | transgenic brassica compestris l. spp. chinensis plants expressing a choline oxidase (coda) gene from arthrobacter globiformis were obtained through agrobacterium tumefaciens-mediated transformation. in the transgenic plants, coda gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (elisa) examination, immunogold localization, and (1)h-nuclear magnetic resonance ((1)h-nmr). stress tolerance was evaluated in the t(3) plants under e ... | 2010 | 21043054 |
| the impact of environmental contamination with antibiotics on levels of resistance in soil bacteria. | this study examined the effects of tetracycline and streptomycin on microorganisms in three different soil habitats: forest soil, agricultural soil, and compost. these antibiotics are commonly used in both medical and veterinary therapy as well as in the production of plant biomass and until quite recently, the production of animal biomass. microcosms were used as model systems in which the number of microorganisms in environments containing different amounts of antibiotics was analyzed. the min ... | 2010 | 21043273 |
| metal inducible activity of the oil palm metallothionein-like gene promoter (mt3-a) in prokaryotes. | reporter gene activity under the regulation of the oil palm metallothionein-like gene, mt3-a promoter was assessed in prokaryotes. vector constructs containing mt3-a promoter with (w1mt3-a) and without (w2mt3-a) five prime untranslated region (5'-utr) fused to ß-glucuronidase (gus) gene in pcambia 1304 vector were produced. 5'-rapid amplification of cdna ends (race) using mrna isolated from escherichia coli and agrobacterium tumefaciens harboring w1mt3-a confirmed that fusion transcripts of mt3- ... | 2010 | 21044862 |
| isolation of heat shock-induced nicotiana tabacum transcription promoters and their potential as a tool for plant research and biotechnology. | transcription promoters of heat shock protein (hsp) genes have been used to control the expression of heterologous proteins in plants and plant cells. to obtain a strong hsp promoter that is functionally active in nicotiana tabacum by-2 cells, we set out to identify a promoter of an endogenous gene showing high activation of expression by heat. an n. tabacum by-2 cell culture was treated for 8 h at 37°c and the cell protein extract analyzed by two-dimensional electrophoresis. a major spot was id ... | 2010 | 21052831 |
| investigating agrobacterium-mediated transformation of verticillium albo-atrum on plant surfaces. | agrobacterium tumefaciens has long been known to transform plant tissue in nature as part of its infection process. this natural mechanism has been utilised over the last few decades in laboratories world wide to genetically manipulate many species of plants. more recently this technology has been successfully applied to non-plant organisms in the laboratory, including fungi, where the plant wound hormone acetosyringone, an inducer of transformation, is supplied exogenously. in the natural envir ... | 2010 | 21060684 |
| structural characterization of a group ii 2/2 hemoglobin from the plant pathogen agrobacterium tumefaciens. | within the 2/2 hemoglobin sub-family, no group ii 2/2hbs from proteobacteria have been so far studied. here we present the first structural characterization of a group ii 2/2hb from the soil and phytopathogenic bacterium agrobacterium tumefaciens (at-2/2hbo). the crystal structure of ferric at-2/2hbo (reported at 2.1å resolution) shows the location of specific/unique heme distal site residues (e.g., his(42)cd1, a residue distinctive of proteobacteria group ii 2/2hbs) that surround a heme-ligande ... | 2010 | 21070893 |
| fluorescence of phytochrome adducts with synthetic locked chromophores. | we performed steady state fluorescence measurements with phytochromes agp1 and agp2 of agrobacterium tumefaciens and three mutants in which photoconversion is inhibited. these proteins were assembled with the natural chromophore biliverdin (bv), with phycoerythrobilin (peb), which lacks a double bond in the ring c-d-connecting methine bridge, and with synthetic bilin derivatives in which the ring c-d-connecting methine bridge is locked. all peb and locked chromophore adducts are photoinactive. a ... | 2010 | 21071442 |
| agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of the cultivated mushroom flammulina velutipes. | agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of flammulina velutipes using a hygromycin b resistance gene as selectable marker. the gene coding for urogen iii methyltransferase (cob) was introduced into f. velutipes dikaryotic cells. the resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in f. velutipes. | 2010 | 21071861 |
| argc orthologs from rhizobiales show diverse profiles of transcriptional efficiency and functionality in sinorhizobium meliloti. | several factors can influence ortholog replacement between closely related species. we evaluated the transcriptional expression and metabolic performance of ortholog substitution complementing a sinorhizobium meliloti argc mutant with argc from rhizobiales (agrobacterium tumefaciens, rhizobium etli, and mesorhizobium loti). the argc gene is necessary for the synthesis of arginine, an amino acid that is central to protein and cellular metabolism. strains were obtained carrying plasmids with argc ... | 2010 | 21075924 |
| qualitative and quantitative detection of agricultural microorganisms expressing iturin and mop cyclase in soils. | the environmental release of genetically engineered microorganisms (gems) to improve agriculture or remediate environmental hazards has raised concern over the fate of the organisms and their engineered genes. to detect the microorganisms released into the environment at the molecular level, bacillus subtilis kb producing iturin and pseudomonas fluorescens mx1 carrying the moc (mannityl opine catabolism) region from the agrobacterium tumefaciens were employed as model microorganisms. using speci ... | 2010 | 21077680 |
| broad-range antagonistic rhizobacteria pseudomonas fluorescens and serratia plymuthica suppress agrobacterium crown gall tumours on tomato plants. | to examine the biocontrol activity of broad-range antagonists serratia plymuthica ic1270, pseudomonas fluorescens q8r1-96 and p. fluorescens b-4117 against tumourigenic strains of agrobacterium tumefaciens and a. vitis. | 2010 | 21091861 |
| identification of virulence genes in the corn pathogen colletotrichum graminicola by agrobacterium tumefaciens-mediated transformation. | a previously developed agrobacterium tumefaciens-mediated transformation (atmt) protocol for the plant pathogenic fungus colletotrichum graminicola led to high rates of tandem integration of the whole ti-plasmid, and was therefore considered to be unsuitable for the identification of pathogenicity and virulence genes by insertional mutagenesis in this pathogen. we used a modified atmt protocol with acetosyringone present only during the co-cultivation of c. graminicola and a. tumefaciens. analys ... | 2011 | 21118348 |
| potassium chloride and rare earth elements improve plant growth and increase the frequency of the agrobacterium tumefaciens-mediated plant transformation. | plant transformation efficiency depends on the ability of the transgene to successfully interact with plant host factors. our previous work and the work of others showed that manipulation of the activity of host factors allows for increased frequency of transformation. recently we reported that exposure of tobacco plants to increased concentrations of ammonium nitrate increases the frequency of both homologous recombination and plant transgenesis. here we tested the influence of kcl and salts of ... | 2010 | 21132499 |
| [culture of hairy roots of panax japonicus and ginsenoside re synthesis]. | to establish the induction method and culture system of hairy roots of panax japonicus, and determine ginsenoside re contents. | 2010 | 21141482 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| rhizobium pusense sp. nov., isolated from rhizosphere of chickpea (cicer arietinum l). | a bacterial strain nrcpb10t was isolated from rhizosphere soil of chickpea (cicer arietinum l), in pusa, new delhi, india.16s rrna gene sequence of the strain nrcpb10t showed the highest similarity 98.9% with rhizobium radiobacter icmp 5785t followed by rhizobium larrymoorei icmp 15237t (97.7%) and rhizobium rubi lmg 17935t (97.4%). phylogenetic analysis of strain nrcpb10t using housekeeping genes reca and atpd revealed their distinct position differing from other recognized rhizobium species. d ... | 2010 | 21148676 |
| a glutamic acid-rich protein identified in verticillium dahliae from an insertional mutagenesis affects microsclerotial formation and pathogenicity. | verticillium dahliae kleb. is a phytopathogenic fungus that causes wilt disease in a wide range of crops, including cotton. the life cycle of v. dahliae includes three vegetative phases: parasitic, saprophytic and dormant. the dormant microsclerotia are the primary infectious propagules, which germinate when they are stimulated by root exudates. in this study, we report the first application of agrobacterium tumefaciens-mediated transformation (atmt) for construction of insertional mutants from ... | 2010 | 21151869 |
| development of a transgenic hairy root system in jute (corchorus capsularis l.) with gusa reporter gene through agrobacterium rhizogenes mediated co-transformation. | transgenic hairy root system is important in several recalcitrant plants, where agrobacterium tumefaciens-mediated plant transformation and generation of transgenic plants are problematic. jute (corchorus spp.), the major fibre crop in indian subcontinent, is one of those recalcitrant plants where in vitro tissue culture has provided a little success, and hence, agrobacterium-mediated genetic transformation remains to be a challenging proposition in this crop. in the present work, a system of tr ... | 2010 | 21153028 |
| molecular insights into plant cell proliferation disturbance by agrobacterium protein 6b. | the agrobacterium ti plasmid (t-dna) 6b proteins interact with many different host proteins implicated in plant cell proliferation. here, we show that arabidopsis plants overexpressing 6b display microrna (mirna) deficiency by directly targeting serrate and ago1 via a specific loop fragment (residues 40-55). in addition, we report the crystal structures of agrobacterium tumefaciens ak6b at 2.1 å, agrobacterium vitis ab6b at 1.65 å, and arabidopsis adp ribosylation factor (arf) at 1.8 å. the 6b s ... | 2010 | 21156810 |
| an efficient foxtail mosaic virus vector system with reduced environmental risk. | plant viral vectors offer high-yield expression of pharmaceutical and commercially important proteins with a minimum of cost and preparation time. the use of agrobacterium tumefaciens has been introduced to deliver the viral vector as a transgene to each plant cell via a simple, nonsterile infiltration technique called "agroinoculation". with agroinoculation, a full length, systemically moving virus is no longer necessary for excellent protein yield, since the viral transgene is transcribed and ... | 2010 | 21162736 |
| reactivation of meristem activity and sprout growth in potato tubers require both cytokinin and gibberellin. | reactivation of dormant meristems is of central importance for plant fitness and survival. due to their large meristem size, potato (solanum tuberosum) tubers serve as a model system to study the underlying molecular processes. the phytohormones cytokinins (ck) and gibberellins (ga) play important roles in releasing potato tuber dormancy and promoting sprouting, but their mode of action in these processes is still obscure. here, we established an in vitro assay using excised tuber buds to study ... | 2010 | 21163959 |
| gene encoding a c-type cyclin in mycosphaerella graminicola is involved in aerial mycelium formation, filamentous growth, hyphal swelling, melanin biosynthesis, stress response, and pathogenicity. | mycosphaerella graminicola is an important wheat pathogen causing septoria tritici blotch. to date, an efficient strategy to control m. graminicola has not been developed. more significantly, we have a limited understanding of the molecular mechanisms of m. graminicola pathogenicity. in this study, we attempted to characterize an mcc1-encoding c-type cyclin, a gene homologous to fcc1 in fusarium verticillioides. four independent mcc1 knock-out mutants were generated via agrobacterium tumefaciens ... | 2011 | 21171890 |
| development of antibiotic marker-free creeping bentgrass resistance against herbicides. | herbicide-resistant creeping bentgrass plants (agrostis stolonifera l.) without antibiotic-resistant markers were produced by agrobacterium-mediated transformation. embryogenic callus tissues were infected with agrobacterium tumefaciens eha105, harboring the bar and the cp4-epsps genes for bialaphos and glyphosate resistance. phosphinothricin-resistant calli and plants were selected. soil-grown plants were obtained at 14-16 weeks after transformation. genetic transformation of the selected, rege ... | 2011 | 21173055 |
| agrobacterium-mediated silencing of caffeine synthesis through root transformation in camellia sinensis l. | tea [camellia sinensis (l.) o. kuntze] is a perennial and most popular non-alcoholic caffeine-containing beverage crop. tea has several constraints for its genetic improvement such as its high polyphenolic content and woody perennial nature. the development of transgenic tea is very difficult, laborious, and time taking process. in tea, regeneration requires minimum 8-12 months. in view of this, attempt has been made in this article to develop a rapid, efficient, and quite economical agrobacteri ... | 2010 | 21181507 |
| production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker. | the use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. mat (multi-auto-transformation) vector system has been one of the different strategies to excise the selection marker gene and produce marker-free transgenic plants. in the present study, ipt (isopentenyl transferase) gene was used as a selection marker gene. ... | 2010 | 21184230 |
| control of lipopolysaccharide biosynthesis by ftsh-mediated proteolysis of lpxc is conserved in enterobacteria but not in all gram-negative bacteria. | despite the essential function of lipopolysaccharides (lps) in gram-negative bacteria, it is largely unknown how the exact amount of this molecule in the outer membrane is controlled. the first committed step in lps biosynthesis is catalyzed by the lpxc enzyme. in escherichia coli, the cellular concentration of lpxc is adjusted by the only essential protease in this organism, the membrane-anchored metalloprotease ftsh. turnover of e. coli lpxc requires a length- and sequence-specific c-terminal ... | 2010 | 21193611 |
| biochemical characteristics of the novel haloalkane dehalogenase data, isolated from the plant pathogen agrobacterium tumefaciens c58. | we report the biochemical characterization of a novel haloalkane dehalogenase, data, isolated from the plant pathogen agrobacterium tumefaciens c58. data possesses a peculiar pair of halide-stabilizing residues, asn-tyr, which have not been reported to play this role in other known haloalkane dehalogenases. data has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chi ... | 2010 | 21193677 |
| agrobacterium tumefaciens-mediated transgenic plant production via direct shoot bud organogenesis from pre-plasmolyzed leaf explants of catharanthus roseus. | production of agrobacterium tumefaciens-mediated transgenic plants, via direct shoot bud organogenesis from leaves of catharanthus roseus, is reported. a. tumefaciens harbouring the plasmid pbi121 with gus gene uida and kanamycin resistance gene nptii was used. highest transformation efficiency of 1.4 transgenic shoots/responded explant was obtained when pre-plasmolysed leaves, pre-incubated on shoot bud induction medium for 10 days, were subjected to sonication for 30 s prior to transformation. ... | 2011 | 21207108 |
| biolistic-mediated transformation protocols for maize and pearl millet using pre-cultured immature zygotic embryos and embryogenic tissue. | maize (zea mays l.) is the most important cereal food crop in sub-saharan africa and latin america, and a key feed crop in asia, whereas pearl millet (pennisetum glaucum (l.) r. br.) is a staple food that supplies a major proportion of calories and protein to large segments of the populations living in the semi-arid tropical regions of africa and asia. the limitations of biological gene transfer with agrobacterium tumefaciens specifically related to recalcitrant cereal crops, led to the developm ... | 2011 | 21207279 |
| agrobacterium tumefaciens-mediated genetic transformation of cereals using immature embryos. | a critical step in the development of a robust agrobacterium tumefaciens-mediated transformation -system for cereal crop plants is the establishment of optimal conditions for efficient t-dna delivery into target tissue, from which plants can be regenerated. although, agrobacterium-mediated transformation of cereals is an important method that has been widely used by many laboratories around the world, routine protocols have been established only in specific cultivars within a species and with sp ... | 2011 | 21207280 |
| highly efficient agrobacterium-mediated transformation of banana cv. rasthali (aab) via sonication and vacuum infiltration. | a reproducible and efficient transformation method was developed for the banana cv. rasthali (aab) via agrobacterium-mediated genetic transformation of suckers. three-month-old banana suckers were used as explant and three agrobacterium tumefaciens strains (eha105, eha101, and lba4404) harboring the binary vector pcambia1301 were used in the co-cultivation. the banana suckers were sonicated and vacuum infiltered with each of the three a. tumefaciens strains and co-cultivated in the medium contai ... | 2011 | 21212957 |
| the integrity of the periplasmic domain of the vira sensor kinase is critical for optimal coordination of the virulence signal response in agrobacterium tumefaciens. | the plant pathogen agrobacterium tumefaciens responds to three main signals at the plant-bacterium interface: phenolics, such as acetosyringone (as), monosaccharides, and acidic ph (∼5.5). these signals are transduced via the chromosomally encoded sugar binding protein chve and the ti plasmid-encoded vira/virg two-component regulatory system, resulting in the transcriptional activation of the ti plasmid virulence genes. here, we present genetic and physical evidence that the periplasmic domain o ... | 2011 | 21216996 |
| agrobacterium tumefaciens-mediated transformation of a taxol-producing endophytic fungus, cladosporium cladosporioides md2. | in this study, an agrobacteriurn tumefaciens-mediated transformation (atmt) protocol was successfully developed for the genetic transformation of a taxol-producing fungus, cladosporium cladosporioides md2, and the co-cultivation conditions affecting the transformation efficiency were optimized. the optimal transformation conditions were that 1 ml of c. cladosporioides md2 spore suspension (10(8) spores/ml) was mixed with an equal volume of a. tumefaciens cultures, which contained 400 μl of a. tu ... | 2011 | 21221971 |