Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| potential nitrosamine formation and its prevention during biological denitrification of red beet juice. | high nitrate intake has been shown to result in an increased risk of endogenous formation of n-nitroso compounds. certain vegetables and vegetable juices contain high concentrations of nitrate. biological denitrification using strains of paracoccus denitrificans (p.d.) has been proposed as effective means to reduce nitrate contents in such vegetable juices. during this bacterial denitrification process, substantial nitrite concentrations are transiently formed. this study investigated whether n- ... | 1997 | 9146735 |
| arabinanase a from pseudomonas fluorescens subsp. cellulosa exhibits both an endo- and an exo- mode of action. | pseudomonas fluorescens subsp. cellulosa expressed arabinanase activity when grown on media supplemented with arabinan or arabinose. arabinanase activity was not induced by the inclusion of other plant structural polysaccharides, and was repressed by the addition of glucose. the majority of the pseudomonas arabinanase activity was extracellular. screening of a genomic library of p. fluorescens subsp. cellulosa dna constructed in lambda zapii, for recombinants that hydrolysed red-dyed arabinan, i ... | 1997 | 9163351 |
| urinary excretion of 3-methyladenine after consumption of fish containing high levels of dimethylamine. | the urinary excretion of the dna alkylation product, 3-methyladenine (3-meade), was measured in human volunteers who were on controlled diets and consumed fresh fish, or frozen-stored fish that contained 50-fold higher levels of dimethylamine (dma), with or without ingested nitrate. dma potentially could react with nitrosating agents in the diet or within the body, and produce the potent carcinogen n-nitrosodimethylamine (ndma), which can then react with dna to form several adducts including 3-m ... | 1997 | 9163693 |
| growth conditions of aspergillus sp. athum-3482 for polygalacturonase production. | a wild type of aspergillus sp. athum-3482 produced extracellular polygalacturonase when grown in liquid medium containing citrus pectin as sole carbon source. a number of factors affecting enzyme activity were investigated. polygalacturonase activities as high as 4.3 u ml-1 (reducing-group-releasing activity) and 17 u ml-1 (viscosity-diminishing activity) were obtained under optimum growth conditions. with sugar-beet as sole carbon source the respective activities were 6.5 u ml-1 and 40 u ml-1, ... | 1997 | 9163956 |
| the satellite rna of barley yellow dwarf virus-rpv is supported by beet western yellows virus in dicotyledonous protoplasts and plants. | the subgroup ii luteovirus barley yellow dwarf virus-rpv (bydv-rpv) acts as a helper virus for a satellite rna (satrpv rna). the subgroup ii luteovirus beet western yellows virus (bwyv) and the st9-associated rna (st9a rna), a bwyv-associated rna that encodes a polymerase similar to those of subgroup i luteoviruses, were assayed for their ability to support replication of satrpv rna. satrpv rna was replicated in tobacco protoplasts in the presence of bwyv rna or a mixture of bwyv plus the st9a r ... | 1997 | 9168880 |
| eukaryotic release factor 1 (erf1) abolishes readthrough and competes with suppressor trnas at all three termination codons in messenger rna. | it is known from experiments with bacteria and eukaryotic viruses that readthrough of termination codons located within the open reading frame (orf) of mrnas depends on the availability of suppressor trna(s) and the efficiency of termination in cells. consequently, the yield of readthrough products can be used as a measure of the activity of polypeptide chain release factor(s) (rf), key components of the translation termination machinery. readthrough of the uag codon located at the end of the or ... | 1997 | 9171074 |
| the impact of diets varying in carbohydrates resistant to endogenous enzymes and lignin on populations of ascaris suum and oesophagostomum dentatum in pigs. | the impact of diets varying in type and level of carbohydrates resistant to endogenous enzymes and lignin on the establishment and location of ascaris suum and oesophagostomum dentatum was investigated experimentally. fifty worm-free pigs, from a specific pathogen-free farm were used. the animals were assigned randomly to 5 diets and infected with 600 infective a. suum eggs and 6000 infective larvae of o. dentatum per pig. the diets consisted of a traditional ground barley plus protein feed (die ... | 1997 | 9172427 |
| influence of particle size on the effectiveness of beet pulp fiber. | sixteen holstein cows in midlactation were used in a design based on a replicated 4 x 4 latin square with the last period removed to determine the influence of particle size of beet pulp neutral detergent fiber (ndf) on its effectiveness as a replacement for alfalfa ndf. diets were a low forage, low fiber diet [12.1 g of alfalfa ndf/100 g of dry matter (dm)], a normal forage diet (low forage plus 7.8 g of additional alfalfa ndf/100 g of dm), and two low forage diets with 5.3 g of ndf/100 g of dm ... | 1997 | 9178130 |
| cloning and sequencing of a cdna encoding alpha-glucosidase from sugar beet. | a cdna encoding sugar beet alpha-glucosidase was cloned from a library constructed from mrna of suspension-cultured cells. the cdna, 3056 bp in length, had an open reading frame encoding a polypeptide of 913 amino acid residues with a molecular mass of 102,078 da, included only one of four regions which were conserved in the alpha-amylase family of enzymes. the deduced amino acid sequence from the analysis of the cdna contained the sequences of the proteolysis peptides and the active site region ... | 1997 | 9178565 |
| evidence for in vitro and in vivo autocatalytic processing of the primary translation product of beet necrotic yellow vein virus rna 1 by a papain-like proteinase. | beet necrotic yellow vein virus rna 1 contains a single long orf corresponding to the theoretical translation product of 237 kda which contains the information necessary for replication of the viral genome. this orf contains a putative papain-like proteinase domain which has been localized, on the basis of sequence alignments, between the helicase and polymerase domains. here we show that the rna 1 primary translation product can be cleaved autocatalytically in vitro into two species of 150 kda ... | 1997 | 9191870 |
| effect of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men. | this study is aimed at investigating the effect of feeding a soluble or partly soluble fibre rich-diet on the apparent absorption and balance of calcium, magnesium, iron and zinc in healthy young men, by using a chemical balance technique. | 1997 | 9192195 |
| a novel subviral agent associated with a geminivirus: the first report of a dna satellite. | numerous plant rna viruses have associated with them satellite (sat) rnas that have little or no nucleotide sequence similarity to either the viral or host genomes but are completely dependent on the helper virus for replication. we report here on the discovery of a 682-nt circular dna satellite associated with tomato leaf curl geminivirus (tlcv) infection in northern australia. this is the first demonstration that satellite molecules are not limited to rna viral systems. the dna satellite (tlcv ... | 1997 | 9192696 |
| accumulation and distribution of free folic acid content in red beet (beta vulgaris l.). | among vegetable plants, red beet contains a relatively high level of the b vitamin folic acid. although many leafy green vegetables contain high levels of folic acid, red beet is consumed primarily as a root vegetable. folic acid levels have been quantified in various vegetable plants, but little information exists regarding the accumulation and distribution of this vitamin in plant tissues. the objective of this study was to characterize free folic acid content (ffac) in shoot and root tissue d ... | 1997 | 9198109 |
| genetic characterization of populations of a de novo arisen sugar beet pest, aubeonymus mariaefranciscae (coleoptera, curculionidae), by rapd analysis. | the weevil aubeonymus mariaefranciscae is an important pest of sugar beet crops in southern spain that was first described as a new species in 1979. we have studied the genetic variability of this insect by rapd analysis of 122 individuals using eight primers. the high resolution provided by random amplified polymorphic dna (rapds), in combination with efficient genetic distance estimators, allowed a very detailed description of the ecology and evolution of the populations of this insect in the ... | 1997 | 9211731 |
| single-chain fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay. | a recombinant enzyme-linked immunosorbent assay (elisa) system, entirely based on antibody fragments, is described here as an attractive alternative to assays using polyclonal antisera or monoclonal antibodies. two expression vectors have been developed for cloning and production of single-chain fv (scfv) fusion proteins suitable as coating and detecting reagents, respectively, in a highly sensitive double antibody sandwich elisa. the coating reagent is produced from the vector pzip1, as a bival ... | 1997 | 9212874 |
| identification of a bacterial pectin acetyl esterase in erwinia chrysanthemi 3937. | erwinia chrysanthemi causes soft-rot diseases of various plants by enzymatic degradation of the pectin in plant cell walls. the structural complexity of pectin requires the combined action of several pectinases for its efficient breakdown. three types of pectinases have so far been identified in e. chrysanthemi: two pectin methyl esterases (pema, pemb), a polygalacturonase (pehx), and eight pectate lyases (pela, pelb, pelc, peld, pele, pell, pelz, pelx). we report in this paper the analysis of a ... | 1997 | 9218776 |
| a nitrogen-free hypocaloric diet and recombinant human growth hormone stimulate postoperative protein synthesis: fasted and fed leucine kinetics in the surgical patient. | twelve otherwise healthy patients undergoing elective surgery for resection of rectosigmoid adenocarcinoma were randomly allocated to two groups: one group receiving intravenous dextrose 5% 600 to 800 kcal.d-1 (dx, n = 6) and the other group receiving the same amount of dextrose intravenously plus recombinant human growth hormone (dx + rgh, n = 6). supplementation with rgh started on the day of surgery and continued postoperatively for 5 days. no nitrogen was provided in the diet. this regimen w ... | 1997 | 9225834 |
| changes in the level and activation state of the plasma membrane h(+)-atpase during aging of red beet slices. | the effect of aging on the plasma membrane (pm) h(+)-atpase of red beet (beta vulgaris l.) parenchyma discs was analyzed in pm purified by aqueous two-phase partitioning. aging increased both the activity in the amount of immunodetectable h(+)-atpase in the pm. the activity assayed at slightly alkaline ph values increased earlier and more strongly than that assayed at acidic ph values, so that the ph curve of the enzyme from aged beet discs was shifted toward more alkaline values. aging decrease ... | 1997 | 9232872 |
| detection of alien chromatin conferring resistance to the beet cyst nematode (heterodera schachtii schm.) in cultivated beet (beta vulgaris l.) using in situ hybridization. | chromatin originating from wild beets of the genus beta, section procumbentes, has been investigated in nematode-resistant hybrid-derived lines of sugar beet (beta vulgaris l.) by in situ hybridization using satellite, telomeric and ribosomal dna repeats, a yeast artificial chromosome (yac) and total genomic dna as probes. the allen chromosome was detected in three monosomic addition lines (2n = 18 + 1) by genomic in situ hybridization. fluorescence in situ hybridization with a genome-specific s ... | 1997 | 9246412 |
| in vivo recombination between two strains of the genus nucleopolyhedrovirus in its natural host, spodoptera exigua. | the dna restriction map for the enzymes bamhi, bglii, psti, and xbai of semnpv-us (se-us), the best-studied nucleopolyhedrovirus strain isolated from spodoptera exigua in california, was used as a reference to construct that of semnpv-sp2 (se-sp2), a closely related spanish strain of the same virus. after coinfection of s. exigua with both the se-us and se-sp2 strains per os, a recombinant virus (semnpv-sur1 [se-sur1]) was detected after one passage which quickly replaced the parental viruses. a ... | 1997 | 9251191 |
| detection and characterization of a distinct type of beet necrotic yellow vein virus rna 5 in a sugarbeet growing area in europe. | a fifth beet necrotic yellow vein virus (bnyvv) rna species has been detected in europe in sugarbeet infected with p-type bnyvv. very little sequence variation was found between two european sources of this rna 5*, but considerable differences were detected between these two european sources on the one hand and the four japanese sources recently analysed by kiguchi et al. on the other. the bnyvv rna 5-encoded 26 k proteins share a stretch of six amino acids (frgpgn) with the bnyvv rna 3-encoded ... | 1997 | 9267459 |
| suppression of potyvirus infection by coexpressed closterovirus protein. | a tobacco etch virus (tev)-based expression vector has been used for insertion of several orfs derived from the unrelated beet yellows virus (byv). hybrid tev variants expressing the byv capsid protein, 20-kda protein, or hsp70 homolog systemically infected nicotiana tabacum and stably retained byv sequences. in contrast, insertion of the orf encoding byv leader proteinase (l-pro) resulted in severely impaired systemic transport and accumulation of recombinant tev. progeny of this virus underwen ... | 1997 | 9268155 |
| the genome-linked protein of potato leafroll virus is located downstream of the putative protease domain of the orf1 product. | the sequence of the 32 n-terminal amino acids of the protein (vpg) which is covalently linked to the rna of potato leafroll virus has been determined. the obtained vpg sequence mapped to position 400 to 431 of the plrv orf1 product, downstream of the putative protease domain and in front of the rna-dependent rna polymerase. comparison with other viral sequences revealed significant similarities with the orf1 products of beet western yellows virus, cucurbit aphid-borne yellows virus, and beet mil ... | 1997 | 9268161 |
| cell wall analysis in aspergillus niger strains characterized by different tolerance to toxic compounds of beet molasses. | aspergillus niger strains, sensitive or resistant to toxic compounds of beet molasses, were the object of the present studies. between the studied strains differences existed in the cell wall dry mass and wall components content, chitin synthesis, activity of enzymes involved in cell wall synthesis, and in the wall ultrastructure. higher content of proteins and lipids but lower of glucan and chitin; less or lack of fibrillar components, thinner cell wall as well as lower level of glucanase and c ... | 1997 | 9271845 |
| resistance to both cyst and root-knot nematodes conferred by transgenic arabidopsis expressing a modified plant cystatin. | plant nematodes are major pests of agriculture. transgenic plant technology has been developed based on the use of proteinase inhibitors as nematode anti-feedants. the approach offers prospects for novel plant resistance and reduced use of environmentally damaging nematicides. a modified rice cystatin, oc-i delta d86, expressed as a transgene in arabidopsis thaliana, has a profound effect on the size and fecundity of females for both heterodera schachtii (beet-cyst nematode) and meloidogyne inco ... | 1997 | 9301094 |
| kinetic analysis of ca2+/k+ selectivity of an ion channel by single-binding-site models. | current-voltage relationships of a cation channel in the tonoplast of beta vulgaris, as recorded in solutions with different activities of ca2+ and k+ (from johannes & sanders 1995, j. membrane biol. 146:211-224), have been reevaluated for ca2+/k+ selectivity. since conversion of reversal voltages to permeability ratios by constant field equations is expected to fail because different ions do not move independently through a channel, the data have been analyzed with kinetic channel models instea ... | 1997 | 9307443 |
| the n-terminal region of the luteovirus readthrough domain determines virus binding to buchnera groel and is essential for virus persistence in the aphid. | luteoviruses and the luteovirus-like pea enation mosaic virus (pemv; genus enamovirus) are transmitted by aphids in a circulative, nonreplicative manner. acquired virus particles persist for several weeks in the aphid hemolymph, in which a groel homolog, produced by the primary endosymbiont of the aphid, is abundantly present. six subgroup ii luteoviruses and pemv displayed a specific but differential affinity for escherichia coli groel and groel homologs isolated from the endosymbiotic bacteria ... | 1997 | 9311800 |
| [investigations in central hessia on the occurrence of estrogenic activity content of cattle feed]. | in 21 farms which requested consultation for reasons of infertility problems feed samples (grass silage, n = 20; cornsilage, n = 19; hay, n = 9; cereal mixture, n = 3, brewers grains, n = 5, ensiled sugar beet tops, n = 1) were drawn for assay of free (aglycon) and glycosidic (glycosid) bound oestrogen activity. following sample preparation and extraction, the oestrogenic activity was determined using a radioreceptorassay and only those samples leading to a 50% displacement of the 3h-estradiol-1 ... | 1997 | 9324454 |
| fiber digestion and weight gain in guinea pigs fed diets containing different fiber sources. | the effects of different fiber sources on feed intake, weight gain and digestibility of fiber were examined in guinea-pigs fed pelleted diets containing alfalfa meal, oaten hay, beet pulp and commercial hay cubes mixed with a basal diet at ratios of 3:1 (75% in the diet), 1:1 (50%) and 1:3 (25%). the basal diet contained 50.0% corn, 4.1% wheat, 22.1% wheat flour and 17.7% corn gluten meal. food intake increased as the amount of fiber source was increased, but not in the case of beet pulp. the mo ... | 1997 | 9353638 |
| rumen fermentation and nutrient flows for cows fed grass and grass supplemented with molassed beet pulp pellets. | an experiment was carried out to determine the effect of a grass diet and a concentrate supplement on rumen fermentation and nutrient flows to the duodenum. perennial ryegrass was cut and fed indoors to eight rumen- and duodenum-cannulated friesian cows with or without 3 kg/d of molassed beet pulp in a randomized design experiment. the dry matter intake of grass was significantly lower for cows fed the concentrate supplement (13.6 vs. 11.5 kg of dry matter/d), but total dry matter and organic ma ... | 1997 | 9361218 |
| evidence for signaling between the phytopathogenic fungus pythium ultimum and pseudomonas fluorescens f113: p. ultimum represses the expression of genes in p. fluorescens f113, resulting in altered ecological fitness. | there is increasing evidence that communication between members of the same species, as well as members of different species, is important for the survival of microorganisms in diverse ecological niches, such as the rhizosphere. to investigate whether the phytopathogen pythium ultimum could alter gene expression in the biocontrol strain pseudomonas fluorescens f113, which protects the roots of sugar beet from the fungus, a screening system was developed to detect differential expression of bacte ... | 1997 | 9361412 |
| influence of a blend of fructo-oligosaccharides and sugar beet fiber on nutrient digestibility and plasma metabolite concentrations in healthy beagles. | to evaluate effects of a blend of fructo-oligosaccharides and sugar beet fiber (4:1) at 3 incorporation rates on nutrient digestibility and plasma glucose, insulin, alpha-aminonitrogen, urea, cholesterol, and triglycerides concentrations measured weekly in nonfed dogs and during a 360-minute period after a meal. | 1997 | 9361885 |
| conformation of the 3'-end of beet necrotic yellow vein benevirus rna 3 analysed by chemical and enzymatic probing and mutagenesis. | secondary structure-sensitive chemical and enzymatic probes have been used to produce a model for the folding of the last 68 residues of the 3'-non-coding region of beet necrotic yellow vein benevirus rna 3. the structure consists of two stem-loops separated by a single-stranded region. rna 3-derived transcripts were produced containing mutations which either disrupted base pairing in the helices or maintained the helices but with alterations in the base pairing scheme. other mutants contained s ... | 1997 | 9365250 |
| mercury-induced conformational changes and identification of conserved surface loops in plasma membrane aquaporins from higher plants. topology of pmip31 from beta vulgaris l. | aquaporins are integral membrane proteins occurring in mammals, plants, and microorganisms, which serve as channels that permit the bidirectional passage of water through cellular membranes. higher plants contain abundant levels of aquaporins in both the tonoplast and plasma membrane. aquaporins contain six transmembrane segments with three surface loops located at the apoplastic face of the membrane and two loops at the cytosolic side. in this study, we probed the topology of plasma membrane aq ... | 1997 | 9388202 |
| comparison of elisa and rt-pcr for the detection of beet yellows closterovirus in plants and aphids. | a reverse-transcription polymerase chain reaction (rt-pcr) was developed to detect beet yellows closterovirus (byv) in plants and single aphids using primers spanning the conserved regions of the published sequences of the coat protein gene and open reading frames 7 and 8. three total rna extraction procedures were examined and all were found to produce rna of sufficient quality for rt-pcr, although the rna extraction kit supplied by flowgen was found to be the most versatile for the extraction ... | 1997 | 9395136 |
| characterization of a putative spodoptera exigua multicapsid nucleopolyhedrovirus helicase gene. | putative baculovirus helicases have been implicated as playing an important role in viral dna replication and host specificity. the spodoptera exigua multicapsid nucleopolyhedrovirus (semnpv) helicase is therefore of interest since the virus only infects the beet army worm. sequence analysis of the semnpv lef5-p39 (mu 46.5-55.1) region, which is collinear with the 39k-lef5 area in autographa californica mnpv (acmnpv), revealed an open reading frame (orf) of 3666 bp potentially encoding a protein ... | 1997 | 9400958 |
| beet soil-borne virus rna 1: genetic analysis enabled by a starting sequence generated with primers to highly conserved helicase-encoding domains. | the complete sequence of the 5834 nucleotides of rna 1 of beet soil-borne furovirus (bsbv, ahlum isolate) was determined using a pcr product obtained with primers to highly conserved coding regions for helicase-like proteins in rna 1 of furo-, hordei- and tobraviruses as a starting sequence. unknown parts of the sequence upstream and downstream of this starting sequence were amplified by means of rt-pcr techniques using combinations of specific and random primers. bsbv rna 1 contains one large o ... | 1997 | 9400965 |
| the faea genes from aspergillus niger and aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides. | we report the cloning and characterization of a gene encoding a ferulic acid esterase, faea, from aspergillus niger and aspergillus tubingensis. the a. niger and a. tubingensis genes have a high degree of sequence identity and contain one conserved intron. the gene product, faea, was overexpressed in wild-type a. tubingensis and a protease-deficient a. niger mutant. overexpression of both genes in wild-type a. tubingensis and an a. niger protease-deficient mutant showed that the a. tubingensis g ... | 1997 | 9406381 |
| cutting, ageing and expression of plant membrane transporters. | the activity and the expression of sucrose, hexose and amino acid transporters were studied with fresh, cut or aged tissues and plasma membrane vesicles (pmv) of mature sugar beet (beta vulgaris l.) leaves. cutting and ageing both induced an increase of the transcripts coding for sucrose transporters and hexose transporters. no significant effect could be detected on the amino acid transporter transcripts with the probe used (aap1). a polyclonal serum directed against the arabidopsis thaliana su ... | 1997 | 9408174 |
| oxidative cross-linking of plasma membrane arabinogalactan proteins. | monoclonal antibodies which recognize carbohydrate in arabinogalactan proteins (agps) have revealed that certain carbohydrate epitopes at the outer plasma membrane surface are developmentally regulated. some epitopes are expressed according to cell position, and agps are thought to play a role in cell-cell interaction during development. this study demonstrates that sugar beet plasma membranes contain two subfamilies of agps, with apparent molecular masses of 82 and 97 kda, and that each subfami ... | 1997 | 9418056 |
| effect of spumol k on cell wall of aspergillus niger strains characterized by different tolerance to toxic compounds of beet molasses. | cell walls in the mycelium of aspergillus niger strains sensitive or resistant to toxic molasses compounds growing in the presence of spumol k were the object of the present studies. although the inhibitory effect of spumol k on the cell wall was noticed in studied mycelia the sensitive strains reacted more strongly. in sensitive strains in spumol k presence lower content of cell wall was accomplished by higher amount of proteins but lower of lipids as well as structural polymers i.e. of glucans ... | 1997 | 9429292 |
| highly efficient induction of sugar beet plant regeneration. | a method for high-frequency in vitro regeneration of adventitious shoots from petiole explants of a tetraploid breeding line 405 of beta vulgaris l. was established. plant regeneration was obtained by direct bud formation from petioles. explants were precultured in a medium containing ms inorganic salts supplemented with gamborg's vitamins, named msb. adventitious shoots were developed when petiole explants excised from 20-30-day-old seedlings grown on msb containing 0.3 mg/l naphthalene acetic ... | 1997 | 9429780 |
| purification and properties of arfi, an alpha-l-arabinofuranosidase from cytophaga xylanolytica. | an alpha-l-arabinofuranosidase (alpha-l-arabinofuranoside arabinofuranohydrolase [ec 3.2.1.55]; referred to below as arfi) from cytophaga xylanolytica xm3 was purified 85-fold by anion-exchange and hydrophobic interaction column chromatography. the native enzyme had a pi of 6.1 and an apparent molecular mass of 160 to 210 kda, and it appeared to be a trimer or tetramer consisting of 56-kda subunits. with p-nitrophenyl-alpha-l-arabinofuranoside as the substrate, the enzyme exhibited a k(m) of 0.5 ... | 1998 | 9435061 |
| regulatory sequences of arabidopsis drive reporter gene expression in nematode feeding structures. | in the quest for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures, we show that promoter tagging is a valuable tool. a large collection of transgenic arabidopsis plants was generated. they were transformed with a beta-glucuronidase gene functioning as a promoter tag. three t-dna constructs, pgv1047, p delta gusbin19, and pmog553, were used. early responses to nematode invasion were of primary interest. six lines exhibiting beta-glucu ... | 1997 | 9437858 |
| suppression of the number of aberrant crypt foci of rat colorectum by ingestion of sugar beet fiber regardless of administration of anti-asialo gm1. | anti-asialo gm1 serum (agm1) reduces natural killer (nk) activity in vitro and in vivo. we investigated the effect of ingestion of sugar beet fiber (sbf) on 1,2-dimethylhydrazine (dmh)-induced aberrant crypt foci (acf) and whether the effect was maintained under nk-reducing conditions by agm1 injection. the ingestion of sbf decreased the number of acf in the colorectum at 4 weeks after treatment with dmh. dietary sbf had a suppressive effect on the formation of acf regardless of the administrati ... | 1997 | 9459172 |
| agelastatins c and d, new pentacyclic bromopyrroles from the sponge cymbastela sp., and potent arthropod toxicity of (-)-agelastatin a. | two new agelastatins, c (3) and d (4), along with the known compound (-)-agelastatin a (1), were isolated from the indian ocean sponge cymbastela sp. the structures were determined by analysis of spectroscopic data and chemical correlation of 4 with a common derivative 5, also prepared from 1. agelastatin a (1) exhibited potent activity against brine shrimp (lc50 1.7 ppm) in addition to insecticidal activity against larvae of beet army worm, spodoptera exigua, and corn rootworm, diabrotica undec ... | 1998 | 9461668 |
| vascular movement of beet necrotic yellow vein virus in beta macrocarpa is probably dependent on an rna 3 sequence domain rather than a gene product. | rnas 1 and 2 of beet necrotic yellow vein virus (bnyvv) carry the functions enabling viral rna replication, cell-to-cell movement, virus assembly and vascular movement of the virus in the systemic host spinacea oleracea. in beta macrocarpa, on the other hand, bnyvv rna 3 is required for vascular movement. replication-competent rna 3 transcripts carrying various point mutations and deletions were coinnoculated with rnas 1 and 2 to young leaves of b. macrocarpa and the ability of the virus to mult ... | 1998 | 9472625 |
| ecbi and ecbr: homologs of luxi and luxr affecting antibiotic and exoenzyme production by erwinia carotovora subsp. betavasculorum. | erwinia carotovora subsp. betavasculorum ecb168 causes vascular necrosis and root rot of sugar beet and produces an antibiotic(s) that is antagonistic against other erwinia spp. ecbi- mutants of ecb168, each containing a single transposon insertion in the ecbi gene (for erwinia carotovora subsp. betavasculorum inducer), do not produce detectable levels of extracellular protease or antibiotic(s), and express less pectate lyase activity and virulence than the wild-type strain. a plasmid containing ... | 1997 | 9476353 |
| osmotic stress induces expression of choline monooxygenase in sugar beet and amaranth. | choline monooxygenase (cmo) catalyzes the committing step in the synthesis of glycine betaine, an osmoprotectant accumulated by many plants in response to salinity and drought. to investigate how these stresses affect cmo expression, a spinach (spinacia oleracea l., chenopodiaceae) probe was used to isolate cmo cdnas from sugar beet (beta vulgaris l., chenopodiaceae), a salt- and drought-tolerant crop. the deduced beet cmo amino acid sequence comprised a transit peptide and a 381-residue mature ... | 1998 | 9489025 |
| investigation with chitosan-oxalate oxidase-catalase conjugate for degrading oxalate from hyperoxaluric rat chyme. | enteric hyperoxaluria manifests due to hyperabsorption of dietary oxalate, secondary to a variety of chronic gastrointestinal disorders. the potential use of chitosan immobilized oxalate oxidase-catalase conjugate to deplete the oxalate content of food materials, while they are in the digestive tract has been evaluated by treating rat stomach chyme with such an enzyme preparation. oxalate oxidase, obtained from beet stem, was adsorbed on chitosan along with catalase and then cross linked with gl ... | 1997 | 9491647 |
| sugar beet fiber in formula diet reduces postprandial blood glucose, serum insulin and serum hydroxyproline. | the object of the study was to investigate the effects of sugar beet fiber in formula diet on blood glucose, serum insulin and serum hydroxyproline in humans. | 1998 | 9505164 |
| effect of inclusion of sugar beet pulp, pelleting and season on laying hen performance. | 1. the effect of three metabolisable energy (me) concentrations 12.2, 11.3 and 10.5 mj me/kg and two forms of diet (mash and pellet) on egg production in summer and winter were studied. the different me values were obtained by diluting the 12.2 mj me/kg diet with sugar beet pulp of 2.88 mj/kg dm. in a 3rd summer experiment the water intake of hens on these diets was examined. data were analysed by factorial analysis of variance, and the relationship between performance and dietary energy concent ... | 1997 | 9510998 |
| the genomic organization of non-ltr retrotransposons (lines) from three beta species and five other angiosperms. | we have isolated and characterized conserved regions of the reverse transcriptase gene from non-ltr retrotransposons, also called long interspersed nuclear elements (lines), from beta vulgaris, b. lomatogona and b. nana. the novel elements show strong homology to other non-ltr retrotransposons from plants, man and animals. lines are present in all species of the genus beta tested, but there was variation in copy number. analysis by southern hybridization and fluorescent in situ hybridization rev ... | 1998 | 9520275 |
| fermentation products of sugar-beet fiber by cecal bacteria lower plasma cholesterol concentration in rats. | plasma cholesterol concentration is reduced by feeding some dietary fibers but the mechanism is not fully understood. we examined whether cecal fermentation products are involved in lowering plasma cholesterol by feeding rats a highly fermentable sugar-beet fiber (sbf) in four separate experiments. these were designed to investigate the effects on plasma cholesterol of oral ingestion of fermentation products on plasma cholesterol, the effects of the products in comparison with that of a short-ch ... | 1998 | 9521629 |
| the plasma-membrane h(+)-atpase from beet root is inhibited by a calcium-dependent phosphorylation. | several plasma-membrane proteins from beet root (beta vulgaris l.) have been functionally incorporated into reconstituted proteoliposomes. these showed h(+)-atpase activity, measured both as atp hydrolysis and h+ transport. the proton-transport specific activity was 10 times higher than in plasma membranes, and was greatly stimulated by potassium and valinomycin. these proteoliposomes also showed calcium-regulated protein kinase activity. this kinase activity is probably due to a calmodulin-like ... | 1998 | 9530879 |
| regulation of a plant plasma membrane sucrose transporter by phosphorylation. | the protein phosphatase inhibitor okadaic acid (oa) either provided directly to sugar beet (beta vulgaris l.) leaf discs or infiltrated in the leaf blade rapidly inhibited sucrose uptake. methyl okadaic acid, a biologically inactive analogue of oa, had only a marginal effect on uptake. oa inhibited proton-motive force-driven uptake of sucrose into plasma membrane vesicles, without affecting their proton permeability. oa did not significantly affect the amount of sucrose transporters present in t ... | 1998 | 9539143 |
| transformation of acinetobacter sp. strain bd413 by transgenic sugar beet dna. | the ability of acinetobacter sp. strain bd413(pfg4 delta nptii) to take up and integrate transgenic plant dna based on homologous recombination was studied under optimized laboratory conditions. restoration of nptii, resulting in kanamycin-resistant transformants, was observed with plasmid dna, plant dna, and homogenates carrying the gene nptii. molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional dna. | 1998 | 9546192 |
| characterization of monoclonal antibodies against cell wall epitopes of the insect pathogenic fungus, nomuraea rileyi: differential binding to fungal surfaces and cross-reactivity with host hemocytes and basement membrane components. | monoclonal antibodies (mabs) were generated against epitopes on yeast-like hyphal bodies and hyphae of the entomopathogenic hyphomycete, nomuraea rileyi. two mabs (4c10, 2h4) bind to epitopes common to both hyphal bodies and hyphae, whereas mab 4e9 binds only to hyphal surfaces. 4c10 and 2h4 appear to be directed towards carbohydrate portions of cell surface mannoproteins, as evidenced by similarities in staining patterns between these mabs and concanavalin a on western blots of n. rileyi cell w ... | 1998 | 9548369 |
| characterization of galactosidases from aspergillus niger: purification of a novel alpha-galactosidase activity. | an enzyme with beta-galactosidase activity and three proteins exhibiting alpha-galactosidase activity were purified from a culture filtrate of aspergillus niger grown on arabinoxylan. beta-galactosidase, optimally active at ph 4 and 60-65 degrees c, was active against p-nitrophenyl-beta-d-galactopyranoside, lactose, and pectic galactan. it was not able to release galactose from sugar beet pectin or lemon pectin. its action on pectic galactan was increased by the presence of beta-galactanase. the ... | 1998 | 9549105 |
| beet yellows closterovirus hsp70-like protein mediates the cell-to-cell movement of a potexvirus transport-deficient mutant and a hordeivirus-based chimeric virus. | it has been suggested that the beet yellows closterovirus (byv)-encoded p65 protein, a homologue of hsp70 cell chaperones, plays a role as a virus movement protein (mp). to test this hypothesis, we used two types of complementation experiments with plant viruses containing the triple gene block (tgb) of mp genes. in one, the byv p65 gene was cloned into a 35s promoter plasmid and introduced into nicotiana benthamiana plants by microprojectile bombardment along with the 35s promoter-driven gus ge ... | 1998 | 9568985 |
| isolation and structural characterisation of rhamnogalacturonan oligomers generated by controlled acid hydrolysis of sugar-beet pulp. | controlled acid hydrolysis was applied to a deesterified beet pulp and the resulting soluble fraction was fractionated on a biorad ag 1x8 column eluted by ammonium acetate ph 6 from 0.05 to 2 m. eight retained fractions were obtained, containing almost exclusively gala and rha. three types of oligomers could be identified: homogalacturonans, of which mono-,di- and tri-gala were isolated as individual components, and two series of rhamnogalacturonan (rg) oligomers. one rg oligomer, isolated after ... | 1997 | 9581279 |
| influence of dietary factors on the ph and ammonia emission of slurry from growing-finishing pigs. | we investigated the effects of dietary factors on the ph and the ammonia emission from slurry of growing-finishing pigs. sixteen male hybrid pigs (80 to 90 kg bw) were allotted to one of four diets based on barley-wheat, tapioca, barley-tapioca, and sugar beet pulp. diets were formulated to have similar ne and cp contents and a similar lysine:ne ratio. diets differed in nonstarch polysaccharide content (nsp) and dietary electrolyte balance (deb). urine and feces were daily collected quantitative ... | 1998 | 9581936 |
| anti-nociceptive and anti-inflammatory effects of some jordanian medicinal plant extracts. | the anti-nociceptive effect of ethanolic extract of 11 traditionally used jordanian plants was studied by using the acetic acid-induced writhing and hot-plate test in mice. the anti-inflammatory effect of these plants was determined by xylene-induced ear oedema in mice and cotton pellet granuloma test in rats. mentha piperita, cinnamomum zeylanicum, apium graveolens, eucalyptus camaldulentis, and ruta graveolens possess an anti-nociceptive effect against both acetic acid-induced writhing and hot ... | 1998 | 9582001 |
| [alternative foods: a critical analysis of a proposal for nutritional intervention] | the nationwide use of a 'multimixture' a formula based on alternative foods such as rice and/or wheat bran, sesame and squash seeds, cassava, beet and carrot leaves, several indigenous leafy vegetables, and ground egg shells has been proposed by the national institute of food and nutrition (inan) as an official solution to fight hunger among poor brazilians. the fragile nutritional state of the target population may make technical or ethical questions appear purely academic, yet nutritional, tox ... | 1998 | 9592227 |
| glutamine synthetase isoenzymes, oligomers and subunits from hairy roots of beta vulgaris l. var. lutea. | a cytosolic and a plastidic isoenzyme of glutamine synthetase (gs; ec 6.3.1.2) were separated from hairy roots of beta vulgaris l. var. lutea. the predominant activity was that of cytosolic gs 1; the relative proportion of plastidic gs 2 activity changed, however, depending on the growth conditions. maximum activity of both isoenzymes was measured after growth with no3- as the major n-source. growth with nh4+ as the sole n-source or growth in constant darkness resulted in a significant decrease ... | 1998 | 9599808 |
| nucleotide sequence and genome organization of grapevine leafroll-associated virus-2 are similar to beet yellows virus, the closterovirus type member. | the entire genome of grapevine leafroll-associated closterovirus-2 (glrav-2), except the exact 5' terminus, was cloned and sequenced. the sequence encompasses nine open reading frames (orfs) which include, in the 5' to 3' direction, an incomplete orf1a encoding a putative viral polyprotein and eight orfs that encode proteins of 52 kda (orf1b), 6 kda (orf2), 65 kda (orf3), 63 kda (orf4), 25 kda (orf5), 22 kda (orf6), 19 kda (orf7) and 24 kda (orf8) respectively, and 216 nucleotides of the 3' untr ... | 1998 | 9603345 |
| nucleotide sequence of the 3'-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus. | the rna genome of grapevine leafroll-associated closterovirus-3 (glrav-3) was cloned as a cdna generated from glrav-3-specific dsrna, and a partial genome sequence of 13154 nucleotides (nt) including the 3' terminus was determined. the sequenced portion contained 13 open reading frames (orfs) potentially encoding, in the 5'-3' direction, proteins of > 77 kda (orf1a; helicase, hel), 61 kda (orf1b; rna-dependent rna polymerase, rdrp), 6 kda (orf2), 5 kda (orf3, small transmembrane protein), 59 kda ... | 1998 | 9603346 |
| detection of nptii (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation. | we have developed a novel system for the sensitive detection of nptii genes (kanamycin resistance determinants) including those present in transgenic plant genomes. the assay is based on the recombinational repair of an nptii gene with an internal 10-bp deletion located on a plasmid downstream of a bacterial promoter. uptake of an nptii gene by transformation restores kanamycin resistance. in escherichia coli, promoterless nptii genes provided by electroporation were rescued with high efficiency ... | 1998 | 9604883 |
| effect of adding sugar beet fibre and wheat bran to a starch diet on the absorption kinetics of glucose, amino-nitrogen and volatile fatty acids in the pig. | the purpose of this study with the pig was to analyse the influence of the type of dietary fibre on quantitative kinetics of the absorption of nutrients deriving from enzymatic digestion in the small intestine and that of volatile fatty acids (vfa) deriving from microbial digestion in the hindgut influenced by the length of adaptation to the diet. two groups of four pigs were fitted with a device for measuring absorption by simultaneous analysis of the differences in the porto-arterial concentra ... | 1998 | 9606749 |
| nucleic acid and protein elimination during the sugar manufacturing process of conventional and transgenic sugar beets. | the fate of cellular dna during the standard purification steps of the sugar manufacturing process from conventional and transgenic sugar beets was determined. indigenous nucleases of sugar beet cells were found to be active during the first extraction step (raw juice production) which was carried out at 70 degrees c. this and the consecutive steps of the manufacturing process were validated in terms of dna degradation by competitive pcr of added external dna. each step of the process proved to ... | 1998 | 9608751 |
| analysis of plastid dna-like sequences within the nuclear genomes of higher plants. | a wide-ranging examination of plastid (pt)dna sequence homologies within higher plant nuclear genomes (promiscuous dna) was undertaken. digestion with methylation-sensitive restriction enzymes and southern analysis was used to distinguish plastid and nuclear dna in order to assess the extent of variability of promiscuous sequences within and between plant species. some species, such as gossypium hirsutum (cotton), nicotiana tabacum (tobacco), and chenopodium quinoa, showed homogenity of these se ... | 1998 | 9615455 |
| genes required for replication of the 15.5-kilobase rna genome of a plant closterovirus. | a full-length cdna clone of beet yellows closterovirus (byv) was engineered and used to map functions involved in the replication of the viral rna genome and subgenomic rna formation. among 10 open reading frames (orfs) present in byv, orfs 1a and 1b suffice for rna replication and transcription. the proteins encoded in these orfs harbor putative methyltransferase, rna helicase, and rna polymerase domains common to sindbis virus-like viruses and a large interdomain region that is unique to clost ... | 1998 | 9621048 |
| the influence of sugar-beet fibre, guar gum and inulin on nutrient digestibility, water consumption and plasma metabolites in healthy beagle dogs. | the aim of the present study was to evaluate the effects of three fibres (sugar-beet fibre, guar gum and inulin) incorporated in the basal diet of healthy dogs at 7 per cent of dry matter (dm). parameters examined included stool output, water consumption, nutrient digestibility and fasting and postprandial plasma metabolites. all fibres increased wet faecal output; an increase in faecal dm output being observed with sugar-beet fibre only. sugar-beet fibre and inulin increased daily water consump ... | 1998 | 9625462 |
| a high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells. | an optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (beta vulgaris l.). a polyethylene glycol-mediated dna transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. the majority of plants were obtained within ... | 1996 | 9631066 |
| genetic relationships within the genus beta determined using both pcr-based marker and dna sequencing techniques. | the sequences of its1 of the internal transcribed spacer regions of nuclear ribosomal dna from 11 species or subspecies in four sections of the genus beta were compared. phylogeny of these wild beet taxa was inferred from the sequence data using phenetic and phylogenetic analyses. multiple accessions from the same 11 taxa were subjected to random amplified polymorphic dna (rapd) analysis, and the data were analysed phenetically. with both molecular techniques and each analysis, three distinctive ... | 1998 | 9650280 |
| cell-to-cell movement of beet necrotic yellow vein virus: i. heterologous complementation experiments provide evidence for specific interactions among the triple gene block proteins. | cell-to-cell movement of beet necrotic yellow vein virus (bnyvv) requires three proteins encoded by a triple gene block (tgb) on viral rna 2. a bnyvv rna 3-derived replicon was used to express movement proteins to functionally substitute for the bnyvv tgb proteins was tested by coinoculation of tgb-defective bnyvv with the various replicons to chenopodium quinoa. trans-heterocomplementation was successful with the movement protein (p30) of tobacco mosaic virus but not with the tubule-forming mov ... | 1998 | 9650295 |
| cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial dna analysis. | mitochondrial (mt) dna restriction fragment length polymorphisms are convenient markers for identifying cytoplasmic variation among plant cultivars. in an attempt to detect new cytoplasmic genotypes useful for sugarbeet breeding, we have compared the hybridization patterns of mtdna from three groups of cultivated beets, viz. leaf beet, garden beet, and fodder beet. utilized as probes were the two sugarbeet mtdna clones that were capable of distinguishing normal fertile and different sources of m ... | 1998 | 9652232 |
| transfer rna mimicry in a new group of positive-strand rna plant viruses, the furoviruses: differential aminoacylation between the rna components of one genome. | recent sequencing of the genomes of several furoviruses--fungus-transmitted rod-shaped positive-strand plant viruses--has suggested the presence of trna-like structures (tlss) at the 3' ends of the genomic rnas. we show here that the genomic rnas of soil-borne wheat mosaic virus (sbwmv), beet soil-borne virus (bsbv), potato mop-top virus (pmtv), peanut clump virus (pcv), and indian peanut clump virus (ipcv) all possess functional tlss that are capable of high-efficiency valylation. while the sbw ... | 1998 | 9657004 |
| nicotiana benthamiana plants expressing beet necrotic yellow vein virus (bnyvv) coat protein-specific scfv are partially protected against the establishment of the virus in the early stages of infection and its pathogenic effects in the late stages of infection. | transgenic plants of nicotiana benthamiana expressing single chain antibody fragments (scfv) specific for the coat protein of beet necrotic yellow vein virus (bnyvv) and non-expressing control plants were inoculated with bnyvv mechanically and by means of the vector polymyxa betae. the scfv were presumably expressed in the endoplasmic reticulum (er). the average time needed for infections to become detectable was longer in the scfv-expressing plants than in the non-expressing control plants. in ... | 1997 | 9672643 |
| generation of monoclonal antibody specific to (1-->5)-alpha-l-arabinan. | a neoglycoprotein (a heptasaccharide of (1-->5)-alpha-l-linked-arabinosyl residues linked to bovine serum albumin) has been used to generate a rat monoclonal antibody specific to a linear chain of (1-->5)-alpha-l-arabinan which is a structural feature of the side chains of pectins. the antibody, designated lm6, detected 100 ng of debranched sugar beet arabinan in an immunodot binding assay and 1 microgram of commercial citrus pectin in a similar assay. hapten inhibition studies indicated that th ... | 1998 | 9675359 |
| a pathogenicity gene isolated from the ppath plasmid of erwinia herbicola pv. gypsophilae determines host specificity. | the host range of the gall-forming bacterium erwinia herbicola pv. gypsophilae (ehg) is restricted to the gypsophila plant whereas e. herbicola pv. betae (ehb) incites galls on beet as well as gypsophila. the pathogenicity of ehg and ehb was previously shown to be dependent on a plasmid (ppath). transposon mutagenesis was used to generate mutants on the cosmid pla150 of the ppath from ehg824-1. a cluster of nonpathogenic mutations flanked by two is1327 elements was identified on a 3.2-kb ndei dn ... | 1998 | 9675891 |
| the synthesis of hsps in sugar beet suspension culture cells under hyperthermia exhibits differential sensitivity to calcium. | experiments aimed at testing the effect of ca2+ on heat shock-induced changes in protein synthesis of cultured sugar beet cells were performed. heat shock inhibits the synthesis of non-heat shock proteins (non-hsps) and promotes the synthesis of a set of hsps. extracellular ca2+ appeared to be strictly required for the synthesis of non-hsps. calcium was found to differentially exert its effect on the hsp synthesis: calcium induced (96 and 76 kda), stimulated (94, 67, 58, 52, 32, 30, 26 and 22 kd ... | 1998 | 9678248 |
| determination of copper and arsenic in refined beet sugar by stripping voltammetry without sample pretreatment. | copper and arsenic have been analysed in refined beet sugar at the microgram kg-1 level by anodic stripping voltammetry (copper) and cathodic stripping voltammetry (arsenic) in the differential-pulse mode (dpasv and dpcsv) at a hanging mercury drop electrode (hmde). dpcsv measurements of arsenic are based on its accumulation onto the hmde as an intermetallic cu-as compound followed by the reduction of as0 to arsine in hydrochloric acid medium. measurements were directly carried out on untreated ... | 1998 | 9684407 |
| dietary carbohydrates alter the fecal composition and ph and the ammonia emission from slurry of growing pigs. | we investigated the effects of dietary carbohydrates on the composition and ph of fecal material and on the ammonia emission from the slurry of growing pigs. thirty-four barrows (bw approximately 40 kg) were randomly allotted to 1 of 10 diets. a basal diet was formulated to meet all requirements for protein, amino acids, minerals, and vitamins. the control diet was composed of the basal diet plus heat-treated cornstarch. in the other diets, the cornstarch in the control diet was replaced with th ... | 1998 | 9690645 |
| analysis of genetic structure and dispersal patterns in a population of sea beet. | a model of the migration pattern in a metapopulation of sea beet (beta vulgaris l. ssp. maritima), based on the continuous distributions of seed and pollen movements, is fitted to gene frequency data at 12 isozyme and rflp loci by maximum likelihood by using an approximation of the simultaneous equilibrium distribution of the gene frequencies generated by the underlying multivariate stochastic process of genetic drift in the population. several alternative restrictions of the general model are f ... | 1998 | 9691051 |
| genome properties of beet virus q, a new furo-like virus from sugarbeet, determined from unpurified virus. | based solely on the information that beet virus q (bvq) contains tubular particles, the entire nucleotide sequence of its tripartite genome was determined from unpurified virus in ca. 40 ml crude sap from locally infected chenopodium quinoa. a starting sequence for rna 1 was generated using primers corresponding to highly conserved helicase domains in the respective rnas of furo-, pomo-, peclu-, hordei- and tobraviruses, and was extended by a walking random-primed cdna approach. the similarity o ... | 1998 | 9714254 |
| the genome organization of the broad bean necrosis virus (bbnv). | the genome of the broad bean necrosis virus oita-isolate (bbnv-o) [rna1 (6.0 kb), rna2 (2.8 kb) and rna3 (2.4 kb)] was cloned and sequenced. computer analysis indicates that methyltransferase, helicase and rna-dependent rna polymerase (rdrp) motifs are present in rna1. the viral capsid protein (cp) cistron is located at the 5' terminal end of rna2 and the mr of cp (20 k) is close to that determined by sds-page analysis. an ochre codon (uaa) in the cp cistron is thought to be partially suppressed ... | 1998 | 9722878 |
| serological characterization of the 3'-proximal encoded proteins of beet yellows closterovirus. | the 3'-proximal open reading frames (orfs) of beet yellows closterovirus, california isolate (byv-ca), were sequenced and the expression of the corresponding proteins analyzed. the nucleotide sequence of orf 5 (coding for p24) was most conserved compared with orf 7 (coding for p20) and orf 8 (coding for p21) among the isolates analyzed. polyclonal antisera were produced to gst fusion proteins of p24, p20, and p21. accumulation of p24, cp, p20 and p21 was studied in infected tetragonia expansa pl ... | 1998 | 9722879 |
| distinct biochemical and topological properties of the 31- and 27-kilodalton plasma membrane intrinsic protein subgroups from red beet. | plasma membrane vesicles from red beet (beta vulgaris l.) storage tissue contain two prominent major intrinsic protein species of 31 and 27 kd (x. qi, c.y tai, b.p. wasserman [1995] plant physiol 108: 387-392). in this study affinity-purified antibodies were used to investigate their localization and biochemical properties. both plasma membrane intrinsic protein (pmip) subgroups partitioned identically in sucrose gradients; however, each exhibited distinct properties when probed for multimer for ... | 1998 | 9733551 |
| [studies for measuring nitrates and nitrites in food served to patients in hospitals from the province of białystok]. | the aim of this study was determination of daily level intake of nitrates and nitrites in the individual meals in the daily food rations of patients from the hospitals from the province of białystok. the studied material were meals and total diets, with regard to selected food products, which were taken to preparing the dishes. the investigation materials were taken from february to june 1996. analysis of nitrates and nitrites contents was realized by the commonly method based on the reaction of ... | 1998 | 9734230 |
| protective effects of fruits and vegetables against in vivo clastogenicity of cyclosphosphamide or benzo[a]pyrene in mice. | seven fruits and 10 vegetables commonly consumed in germany were investigated for their anticlastogenic potencies against cyclophosphamide (cp) and benzo[a]pyrene (bap) in the in vivo mouse bone marrow micronucleus assay. we detected protective effects in 76.5% and 70.6% of the samples, respectively, and more or less distinct quantitative differences between the various plant materials and the two clastogens investigated. with respect to cp, moderate activities were exerted by sweet cherries, st ... | 1998 | 9734714 |
| characterization of a red beet protein homologous to the essential 36-kilodalton subunit of the yeast v-type atpase. | v-type proton-translocating atpases (v-atpases) (ec 3.6.1.3) are electrogenic proton pumps involved in acidification of endomembrane compartments in all eukaryotic cells. v-atpases from various species consist of 8 to 12 polypeptide subunits arranged into an integral membrane proton pore sector (vo) and a peripherally associated catalytic sector (v1). several v-atpase subunits are functionally and structurally conserved among all species examined. in yeast, a 36-kd peripheral subunit encoded by ... | 1998 | 9742042 |
| a convert to fructans in sugar beet. | 1998 | 9743111 | |
| high level fructan accumulation in a transgenic sugar beet. | we have transformed sugar beet into a crop that produces fructans. the gene encoding 1-sucrose:sucrose fructosyl transferase (1-sst), which was isolated from helianthus tuberosus, was introduced into sugar beet. in h. tuberosus, 1-sst mediates the first steps in fructan synthesis through the conversion of sucrose (gf) into low molecular weight fructans gf2, gf3, and gf4. in the taproot of sugar beet transformed with the 1-sst gene, the stored sucrose is almost totally converted into low molecula ... | 1998 | 9743117 |
| isolation, identification, and biological activity of isopersin, a new compound from avocado idioblast oil cells | a new compound, (12z,15z)-1-hydroxy-4-oxo-heneicosa-12,15-dien-2-yl acetate, isopersin (2), has been isolated from avocado idioblast oil cells. in artificial diet bioassays, 2 showed no effects on either larval survivorship or growth of early-instar beet armyworm spodoptera exigua. in contrast, the isomeric persin (1), (12z, 15z)-1-acetoxy-2-hydroxy-4-oxo-heneicosa-12,15-diene, reduces larval growth at equivalent concentrations (500 &mgr;g g-1). compound 2 is not very stable and isomerizes readi ... | 1998 | 9748393 |
| secondary metabolite- and endochitinase-dependent antagonism toward plant-pathogenic microfungi of pseudomonas fluorescens isolates from sugar beet rhizosphere | forty-seven isolates representing all biovars of pseudomonas fluorescens (biovars i to vi) were collected from the rhizosphere of field-grown sugar beet plants to select candidate strains for biological control of preemergence damping-off disease. the isolates were tested for in vitro antagonism toward the plant-pathogenic microfungi pythium ultimum and rhizoctonia solani in three different plate test media. mechanisms of fungal inhibition were elucidated by tracing secondary-metabolite producti ... | 1998 | 9758768 |
| a ca2+- and voltage-dependent cation channel in the nuclear envelope of red beet. | the patch-clamp technique was applied to study ion conductances in various configurations of the nuclear envelope of non-enzyme-treated red beet (beta vulgaris l.) nuclei. with excised patches a non-selective cation channel was observed, that was activated by micromolar concentrations of ca2+ on the nucleoplasmic side of the envelope. the channel activity was also voltage-dependent and the voltage threshold of channel activation changed with the nucleoplasmic ca2+ concentration. the most promine ... | 1998 | 9767144 |
| effect of carbon source on production of alpha-l-arabinofuranosidase by aureobasidium pullulans | a color-variant strain of aureobasidium pullulans (nrrl y-12974) produced alpha-l-arabinofuranosidase (alpha-l-afase) when grown in liquid culture on sugar beet arabinan, wheat arabinoxylan, l-arabinose, l-arabitol, xylose, xylitol, oat spelt xylan, corn fiber, or arabinogalactan. l-arabinose was most effective for production of both whole-broth and extracellular alpha-l-afase activity, followed by l-arabitol. oat spelt xylan, sugar beet arabinan, xylose, xylitol, and wheat arabinoxylan were int ... | 1998 | 9767714 |
| the effect of chard (beta vulgaris l. var. cicla) on the skin of streptozotocin induced diabetic rats. | chard (beta vulgaris l. var. cicla) is one of the plants used as hypoglycaemic agent by diabetics in turkey and it has been reported to reduce blood glucose. the purpose of this study was to investigate the effect of feeding chard on diabetes induced impairments in rat skins. uncontrolled induced diabetes caused significant increases in nonenzymatic glycosylation of skin proteins, lipid peroxidation and blood glucose. administration of chard extract inhibited these effects except the increase in ... | 1998 | 9770212 |
| specific inclusion bodies are associated with replication of lettuce infectious yellows virus rnas in nicotiana benthamiana protoplasts. | nicotiana benthamiana mesophyll protoplasts, either mock-inoculated or inoculated using in vitro transcripts derived from lettuce infectious yellows virus (liyv) rna 1- and/or rna 2-cloned cdnas were analysed by transmission electron microscopy (tem) and, in some cases, also by immunogold labelling. tem revealed the main cytopathological effects of liyv infections in n. benthamiana protoplasts infected with rnas 1 and 2: (a) typical closterovirus-induced (beet yellows virus-type) accumulations o ... | 1998 | 9780036 |