Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| heat shock protein 70 kda (hsp70) increase in sea bass (dicentrarchus labrax, l 1758) thymus after vaccination against listonella anguillarum. | heat shock proteins 70 kda (hsp70) and apoptosis were investigated in thymus of sea bass juveniles (dicentrarchus labrax) subsequently to a vaccination against listonella (syn. vibrio) anguillarum. hsp70 expression was measured by immunohistochemistry and immunoenzymatic methods, resulting in increase in hsp70 after bath immunization and persistent in fish exposed to an intraperitoneal (i.p.) booster. the hsp70 increase in thymus was suggested as induction in lymphocytic cells, to be related to ... | 2013 | 23053607 |
| intracellular cu/zn superoxide dismutase (cu/zn-sod) from hard clam meretrix meretrix: its cdna cloning, mrna expression and enzyme activity. | hard clam (meretrix meretrix) is an economically important bivalve in china. in the present study, a gene coding for an intracellular cu/zn-sod was cloned and characterized from hard clam. the full-length cdna of this cu/zn-sod (designated as mm-iccuzn-sod) consisted of 1,383 bp, with a 462-bp of open reading frame (orf) encoding 153 amino acids. several highly conserved motifs, including the cu/zn binding sites [h(46), h(48), h(63), and h(119) for cu binding; h(63), h(71), h(80), and d(83) for ... | 2012 | 23053968 |
| immune response of turbot (scophthalmus maximus l.) to a broad spectrum vaccine candidate, recombinant glyceraldehyde-3-phosphate dehydrogenase of edwardsiella tarda. | edwardsiella tarda is a gram-negative pathogen which causes systemic infection in turbot. the increasing frequency of edwardsiellosis in turbot farming has stressed the need to understand the immune responses of fish, for the further development of prevention and control strategies. as a broad spectrum protective antigen, a recombinant glyceraldehyde-3-phosphate dehydrogenase (gapdh) of e. tarda eib202 has been proven to present remarkable protection against e. tarda, aeromonas hydrophila, vibri ... | 2012 | 23084627 |
| luminal uptake of vibrio (listonella) anguillarum by shed enterocytes--a novel early defence strategy in larval fish. | as adhesion and translocation through fish gut enterocytes of the pathogen vibrio (listonella) anguillarum are not well investigated, the effective cause of disease and mortality outbreaks in larval sea bass, dicentrarchus labrax, suffering from vibriosis is unknown. we detected v. anguillarum within the gut of experimentally infected gnotobiotic sea bass larvae using transmission electron microscopy and immunogold labelling. intact bacteria were observed in close contact with the apical brush b ... | 2013 | 23121135 |
| toxicity of forest fire retardant chemicals to stream-type chinook salmon undergoing parr-smolt transformation. | long-term fire retardants are used to prevent the spread of wildland fires. these products are normally applied by aircraft and are intended specifically for terrestrial application, but fire retardants have entered aquatic habitats by misapplication and/or accidental spills and have resulted in fish mortalities. the authors examined the toxicity of two fire retardant products, phos-chek 259f and lc-95a, to salmon undergoing parr-smolt transformation. yearling stream-type chinook salmon at the s ... | 2013 | 23161484 |
| the molecular characterization of a catalase from chinese mitten crab eriocheir sinensis. | catalase (cat) is an antioxidant enzyme and plays a significant role in the protection against oxidative stress by reducing hydrogen peroxide. the cat cdna of eriocheir sinensis (escat) was cloned via race technique. the complete sequence of escat cdna consisted of a 5' untranslated regions (utr) of 224 bp, a 3' utr of 1287 bp with a poly (a) tail and an open reading frame (orf) of 1542 bp, which encoded a polypeptide of 513 amino acid residues with a calculated molecular mass of approximately 5 ... | 2013 | 23171400 |
| synthesis and antibacterial activity of conjugates between norfloxacin and analogues of the siderophore vanchrobactin. | from synthetic functionalized analogues of vanchrobactin, a siderophore produced by the fish pathogenic bacteria vibrio anguillarum serotype o2, several vanchrobactin analogues-norfloxacin conjugates were obtained and their antimicrobial activities against the wild-type and mutant strains of vibrio anguillarum serotype o2 have been determined. | 2013 | 23182214 |
| determining vaccination frequency in farmed rainbow trout using vibrio anguillarum o1 specific serum antibody measurements. | despite vaccination with a commercial vaccine with a documented protective effect against vibrio anguillarum o1 disease outbreaks caused by this bacterium have been registered among rainbow trout at danish fish farms. the present study examined specific serum antibody levels as a valid marker for assessing vaccination status in a fish population. for this purpose a highly sensitive enzyme-linked immunosorbent assay (elisa) was developed and used to evaluate sera from farmed rainbow trout vaccina ... | 2012 | 23185402 |
| nigribactin, a novel siderophore from vibrio nigripulchritudo, modulates staphylococcus aureus virulence gene expression. | staphylococcus aureus is a serious human pathogen that employs a number of virulence factors as part of its pathogenesis. the purpose of the present study was to explore marine bacteria as a source of compounds that modulate virulence gene expression in s. aureus. during the global marine galathea 3 expedition, a strain collection was established comprising bacteria that express antimicrobial activity against vibrio anguillarum and/or staphylococcus aureus. within this collection we searched col ... | 2012 | 23203279 |
| c312m: an attenuated vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine. | vibrio anguillarum, a gram-negative bacterial pathogen, is the causative agent of vibriosis that affects a wide range of aquatic animals. in this study, we obtained a mutant v. anguillarum, c312m, derived from the pathogenic v. anguillarum c312 by selection of rifampicin resistance. c312m was slower in growth than the wild type c312, particularly under conditions of iron depletion. compared to c312, c312m was altered in protein production profile and exhibited a dramatically increased median let ... | 2012 | 23209076 |
| fermentation preparation of recombinant vibrio anguillarum vaccine with heterogeneous antigen display. | in the design of recombinant bacterial vector vaccine, heterogeneous antigen is displayed on the outer membrane of the vector strain to evoke polyvalent immunological protection. thus, the expression of heterogeneous antigen in cells and its display on the outer membrane are of great concern for vaccine preparation. in our previous work, a multivalent bacterial vector vaccine mvav6203a-1 was constructed by displaying the protective antigen gapdh from aeromonas hydrophila on the surface of an att ... | 2013 | 23215656 |
| shrimp myd88 responsive to bacteria and white spot syndrome virus. | the myeloid differentiation factor 88 (myd88) is an important adapter protein which links members of the toll-like receptor (tlr) to the downstream components to activate related signaling pathways. in the present study, a myd88 homolog (fcmyd88) was cloned from penaeid shrimp fenneropenaeus chinensis. the orf of fcmyd88 consisted of 1434 bp encoding a polypeptide of 477 amino acids which contains a death domain (dd) and a typical tlr and interleukin-1 receptor (il-1r)-related (tir) domain. homo ... | 2013 | 23238389 |
| miiuy croaker (miichthys miiuy) peroxiredoxin2: molecular characterization, genomic structure and immune response against bacterial infection. | peroxiredoxin2 (prx2) protein is an important member in cellular antioxidant protein superfamily. prx2 exists widely in prokaryotes and eukaryotes, it not only plays a part in eliminate reactive oxygen, but also takes effect in many other metabolic activities, such as stimulate epithelial cell proliferation, participate in the signal transduction in cells and so on. after molecular cloning we got that the complete cdna sequence of prx2 consists 882 bp, including a 5'-utr of 46 bp, an open readin ... | 2013 | 23253493 |
| comparative study of four flagellins of vibrio anguillarum: vaccine potential and adjuvanticity. | vibrio anguillarum is the etiological agent of vibriosis, an aquaculture disease that affects a wide range of farmed fish. the genome of v. anguillarum contains five flagellin genes, i.e. flaa, flab, flac, flad, and flae. in this study, we analyzed the vaccine potential and adjuvanticity of flaa, flab, flad, and flae in a model of japanese flounder (paralichthys olivaceus). for this purpose, recombinant flaa, flab, flad, and flae were expressed in and purified from escherichia coli. in vivo immu ... | 2013 | 23253494 |
| diversification of the expanded teleost-specific toll-like receptor family in atlantic cod, gadus morhua. | toll-like receptors (tlrs) are major molecular pattern recognition receptors of the innate immune system. atlantic cod (gadus morhua) is the first vertebrate known to have lost most of the mammalian tlr orthologues, particularly all bacterial recognising and other cell surface tlrs. on the other hand, its genome encodes a unique repertoire of teleost-specific tlrs. the aim of this study was to investigate if these duplicate tlrs have been retained through adaptive evolution to compensate for the ... | 2012 | 23273344 |
| a four-crd c-type lectin from chlamys farreri mediating nonself-recognition with broader spectrum and opsonization. | c-type lectins are a superfamily of ca(2+)-dependent carbohydrate-recognition proteins consisting of at least one carbohydrate-recognition domain (crd), which participate in nonself-recognition and clearance of invaders. in invertebrate, some multidomain c-type lectins have been identified, but their relative functions and binding mechanism are still meager. in the present study, a c-type lectin (cflec-4) with four crds from chlamys farreri was selected to investigate its possible function in in ... | 2012 | 23276881 |
| innovative vaccination protocol against vibriosis in dicentrarchus labrax (l.) juveniles: improvement of immune parameters and protection to challenge. | the effect of vaccination on immune parameters of european sea bass, dicentrarchus labrax, is not fully established, as well as surveyed throughout rearing till the commercial size. furthermore, available information on the possible role of booster treatments is scarce. sea bass juveniles were vaccinated against listonella anguillarum using a commercial bivalent formulation administered by immersion (priming: 95 dph; booster: 165 dph) or by immersion (priming: 95 dph; booster: 165 dph) and subse ... | 2012 | 23277096 |
| the hydrocarbon-degrading marine bacterium cobetia sp. strain mm1ida2h-1 produces a biosurfactant that interferes with quorum sensing of fish pathogens by signal hijacking. | biosurfactants are produced by hydrocarbon-degrading marine bacteria in response to the presence of water-insoluble hydrocarbons. this is believed to facilitate the uptake of hydrocarbons by bacteria. however, these diffusible amphiphilic surface-active molecules are involved in several other biological functions such as microbial competition and intra- or inter-species communication. we report the isolation and characterization of a marine bacterial strain identified as cobetia sp. mm1ida2h-1, ... | 2013 | 23279885 |
| a novel c-type lectin from crab eriocheir sinensis functions as pattern recognition receptor enhancing cellular encapsulation. | c-type lectins are a large family of ca²⁺-dependent carbohydrate binding proteins which play crucial roles to recognize and eliminate pathogens in innate immunity. in the present study, a novel c-type lectin was identified from eriocheir sinensis (designated as esctl). the full-length cdna of esctl was of 789 bp with an open reading frame of 468 bp encoding a polypeptide of 156 amino acids with a signal sequence and single carbohydrate-recognition domain (crd). the potential tertiary structure o ... | 2013 | 23296116 |
| the anguibactin biosynthesis and transport genes are encoded in the chromosome of vibrio harveyi: a possible evolutionary origin for the pjm1 plasmid-encoded system of vibrio anguillarum? | many vibrio anguillarum serotype o1 strains carry 65-kb pjm1-type plasmids harboring genes involved in siderophore anguibactin biosynthesis and transport. the anguibactin system is an essential factor for v. anguillarum to survive under iron-limiting conditions, and as a consequence, it is a very important virulence factor of this bacterium. our comparative analysis of genomic data identified a cluster harboring homologs of anguibactin biosynthesis and transport genes in the chromosome of vibrio ... | 2013 | 23335587 |
| genome-wide analyses of amphioxus micrornas reveal an immune regulation via mir-92d targeting c3. | recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. however, little is known about how micrornas (mirnas) are involved in the immune defense in amphioxus. in this article, we present a systematic study of amphioxus mirnas in the acute-phase response to bacterial infection; mir-92d was found to regulate the complement pathway in this basal chordate. we identified all 155 possible mirnas present in the amphioxus branchiostoma belcheri genome by b ... | 2013 | 23335747 |
| two abc transporter systems participate in siderophore transport in the marine pathogen vibrio anguillarum 775 (pjm1). | orf40 (named fate) in the vibrio anguillarum pjm1 plasmid-encoding anguibactin iron transport systems is a homolog of atpase genes involved in ferric-siderophore transport. mutation of fate did not affect ferric-anguibactin transport, indicating that there must be other atpase gene(s) in addition to fate. by searching the genomic sequence of v. anguillarum 775(pjm1), we identified a homolog of fate named fvte on chromosome 2. it is of interest that in this locus, we also identified homologs of f ... | 2013 | 23350649 |
| bacterial pathogens and flora isolated from farm-cultured eels (anguilla japonica) and their environmental waters in korean eel farms. | the purpose of this study was to investigate bacterial pathogens and flora in both sick and clinically healthy eels, anguilla japonica, and the environmental rearing waters of korean eel farms. between 2003 and 2010, a total of 621 sick eels were submitted for diagnosis, while 216 healthy eels and 87 environmental water samples were collected during a survey of 26 eel farms in korea. seven different bacterial species were obtained from 183 isolates, which were recovered from the internal organs ... | 2012 | 23357049 |
| [cloning and expression analysis of cathepsin l cdna of exopalaemon carinicauda]. | based on the est sequence from a hemocyte cdna library, the cathepsin l cdna of exopalaemon carinicauda (eccatl) was cloned by rapid amplification of cdna ends (race). the eccatl cdna was 1136 bp in length, which contains an open reading frame (orf) of 960 bp, encoding a 319 amino-acid polypeptide. homology analysis revealed that the amino acid sequence of eccatl was highly conserved with its homologs in other crustaceans. the similarities of eccatl with the catl of palaemonetes varians and pand ... | 2013 | 23389977 |
| molecular characterization of an il-1β gene from ayu, plecoglossus altivelis. | il-1β plays a crucial role as a prototypical proinflammatory cytokine in immune responses and has been shown to affect macrophage functions. however, the effects of putative il-1β homologs on fish macrophages are still less known. here, we cloned the full-length cdna sequence of il-1β (ail-1β) gene from ayu, plecoglossus altivelis. phylogenetic analysis indicated that ail-1β was closest to that of atlantic salmon (salmo salar). real-time quantitative pcr (rt-qpcr) revealed that ail-1β transcript ... | 2013 | 23428516 |
| identification and characterization of four ferritin subunits involved in immune defense of the yesso scallop (patinopecten yessoensis). | as a primary iron storage protein, ferritin plays a vital role in iron homeostasis and innate immunity. in this study, four ferritin subunits (pyfer1, pyfer2, pyfer3, and pyfer4) were cloned from the yesso scallop, patinopecten yessoensis, by rapid amplification of cdna ends (race) following in silico transcriptome analysis. the full-length cdnas of the four ferritins are 895, 920, 891, and 1400 bp in length, respectively, and each contains a putative iron response element (ire) in its 5' utr. m ... | 2013 | 23428517 |
| the response of mrna expression upon secondary challenge with vibrio anguillarum suggests the involvement of c-lectins in the immune priming of scallop chlamys farreri. | the enhanced immunity against a second encounter with the particular pathogen has suggested the presence of "immune priming" in scallop. in the present study, the survival rate and expression patterns of five c-lectin isoforms from scallop chlamys farreri were explored after "vaccination" of heat-killed vibrio anguillarum or successively challenge with v. anguillarum and micrococcus luteus. when scallops were challenged with live bacteria, the survival rate increased significantly only in the gr ... | 2013 | 23434464 |
| overexpression of a c-type lectin enhances bacterial resistance in red swamp crayfish procambarus clarkii. | c-type lectins play important roles in the innate immune system of crustaceans. in this study, a novel c-type lectin gene, designated as pclec4, was obtained from the red swamp crayfish (procambarus clarkii). quantitative real-time polymerase chain reaction revealed that pclec4 is mainly expressed in the crayfish hepatopancreas and intestine, and the pclec4 mrna expression is upregulated after challenged with the bacteria vibrio anguillarum. pclec4 was recombinantly expressed in escherichia coli ... | 2013 | 23435178 |
| characterization of p2x7r and its function in the macrophages of ayu, plecoglossus altivelis. | p2x purinoceptor 7 (p2x7r), an atp-gated ion channel, plays an important role during the innate immune response in mammals. however, relatively little is known about the role of p2x7r in the fish immune system. here, we cloned a cdna sequence encoding ayu (plecoglossus altivelis) p2x7r (ap2x7r). the predicted protein was composed of 574 amino acid residues with a p2x family signature, two transmembrane domains, and a long c-terminal. ap2x7r transcripts were mainly distributed in ayu immune tissu ... | 2013 | 23437395 |
| the first toll receptor from the triangle-shell pearl mussel hyriopsis cumingii. | toll receptor was first discovered in drosophila and has an important function in the innate immunity of invertebrates. in this study, the toll receptor hctoll1 from hyriopsis cumingii with a full length of 3810 bp consisting of a 3687 bp orf that encodes a total of 1228 amino acids protein was selected for further study. the hctoll1 protein consisted of a signal peptide, 17 lrr domains, 2 lrrct domains, 1 lrrnt domain, 1 tm domain, and 1 tir domain. phylogenetic analysis results showed that hct ... | 2013 | 23454418 |
| proteomic characterization of the acute-phase response of yellow stingrays urobatis jamaicensis after injection with a vibrio anguillarum-ordalii bacterin. | systemic inflammatory responses of mammals and bony fish are primarily driven by coordinated up-regulation and down-regulation of plasma acute-phase proteins. although this general principle is believed to be universal among vertebrates, it remains relatively unexplored in elasmobranchs. the objective of this study was to characterize acute changes in the plasma proteome of three yellow stingrays urobatis jamaicensis following intraperitoneal injection with a commercial vibrio bacterin. changes ... | 2013 | 23470813 |
| environmental isolates p1sw and v3sw as a bivalent vaccine induce effective cross-protection against edwardsiella tarda and vibrio anguillarum . | edwardsiella tarda and vibrio anguillarum are severe fish pathogens. in this study, we aimed at selecting avirulent environmental isolates with application potential in the prevention of e. tarda- and v. anguillarum-associated diseases. for this purpose, we selected and analyzed 2 seawater isolates, p1sw and v3sw, belonging to the genera pseudomonas and vibrio, respectively. when administered to turbot scophthalmus maximus via immersion and oral feeding, p1sw and v3sw at a dose of 2 × 108 colony ... | 2013 | 23482384 |
| toll-like receptors and myd88 adaptors in mytilus: complete cds and gene expression levels. | tlr- and myd88-related sequences have been previously investigated in mytibase and then in new transcript reads obtained by illumina technology from the mussel, mytilus galloprovincialis. based on full cds and domain organizations of virtual translations, we identified 23 toll-like receptors (tlrs) and 3 myd88 adaptors. mgtlrs can be arranged in 4 clusters according to extra-cellular lrr domain content. mgtlr-b, -i and -k were the only ones containing a multiple cysteine cluster (mcctlr), a doma ... | 2013 | 23485525 |
| investigating a possible role for the bacterial signal molecules n-acylhomoserine lactones in balanus improvisus cyprid settlement. | increased settlement on bacterial biofilms has been demonstrated for a number of marine invertebrate larvae, but the nature of the cue(s) responsible is not well understood. we tested the hypothesis that the bay barnacle balanus improvisus utilizes the bacterial signal molecules n-acylhomoserine lactones (ahls) as a cue for the selection of sites for permanent attachment. single species biofilms of the ahl-producing bacteria vibrio anguillarum, aeromonas hydrophila and sulfitobacter sp. br1 were ... | 2013 | 23506419 |
| molecular characterization of two kazal-type serine proteinase inhibitor genes in the surf clam mesodesma donacium exposed to vibrio anguillarum. | this study reports two kazal-type serine protease inhibitors (kpi) identified in a cdna library from the surf clam mesodesma donacium, and characterized through rapid amplification of cdna ends (race). the kpis, denoted as mdspi-1 and mdspi-2, presented full sequences of 1139 bp and 781 bp respectively. mdspi-1 had a 5'untranslated region (utr) of 175 bp, a 3'utr of 283 bp and an open reading frame (orf) of 681 pb that encodes for 227 amino acids. mdspi-2 showed a 5'utr of 70 bp, a 3'utr of 279 ... | 2013 | 23528874 |
| a lysin motif (lysm)-containing protein functions in antibacterial responses of red swamp crayfish, procambarus clarkii. | lysin domain (lysm) is a widely spread domain in nature and could bind different peptidoglycans and chitin-like compounds in bacteria and eukaryotes. in plants, lysin motif containing proteins are one of the major classes of pattern recognition proteins which can recognize glcnac-containing glycans and have important functions in plant immunity. however, their functions in animal immunity are still unclear. in this study, a cdna encoding a lysm containing protein was identified from red swamp cr ... | 2013 | 23529009 |
| molecular cloning, characterization and expression analysis of a novel wap65-1 gene from plecoglossus altivelis. | warm temperature acclimation associated 65-kda protein 1 (wap65-1) is a specific fish plasma glycoprotein that is possibly involved in various physiological or pathological processes. in this study, we obtained the cdna and genomic dna sequences of the plecoglossus altivelis wap65-1 (pawap65-1) gene. multiple sequence alignment showed that pawap65-1 is similar in structure to wap65-1 in fish. phylogenetic analysis revealed that pawap65-1 is most closely related to that of a rainbow trout. pawap6 ... | 2013 | 23545468 |
| identification and characterization of a serine protease inhibitor esserpin from the chinese mitten crab eriocheir sinensis. | serine protease inhibitors (serpins) represent an expanding superfamily of endogenous inhibitors that regulate proteolytic events and involve in a variety of physiological processes. a serine protease inhibitor, namely esserpin, was identified from chinese mitten crab eriocheir sinensis based on expressed sequence tag (est) analysis. the full-length cdna of esserpin was of 2367 bp, including an open reading frame (orf) of 1371 bp encoding a polypeptide of 456 amino acids with estimated molecular ... | 2013 | 23567854 |
| molecular cloning, phylogenetic analysis and functional characterization of soluble toll-like receptor 5 in gilthead seabream, sparus aurata. | two forms of tlr5, one membrane-anchored and one soluble, have been described in some teleost fish species. however, the exact role of each form has been poorly studied. in the present study, we show that the mrna levels of soluble gilthead seabream tlr5 (sbtlr5s) are highly induced in head kidney, spleen, liver and blood after vibrio anguillarum infection, suggesting an important role for sbtlr5s in the innate immune response against bacteria. comparative genomic and phylogenetic analyses revea ... | 2013 | 23571319 |
| bax inhibitor-1 silencing suppresses white spot syndrome virus replication in red swamp crayfish, procambarus clarkii. | bax inhibitor-1 (bi-1) was originally described as an anti-apoptotic protein in both animal and plant cells. bi-1 overexpression suppresses er stress-induced apoptosis in animal cells. inhibition of bi-1 activity could induce the cell death in mammals and plants. however, the function of bi-1 in crustacean immunity was unclear. in this paper, the full-length cdna of a bi-1 protein in red swamp crayfish, procambarus clarkii (pcbi-1) was cloned and its expression profiles in normal and infected cr ... | 2013 | 23583724 |
| chemical shift assignments and secondary structure prediction of the phosphorelay protein vanu from vibrio anguillarum. | vibrio anguillarum is a biofilm forming gram-negative bacterium that survives prolonged periods in seawater and causes vibriosis in marine life. a quorum-sensing signal transduction pathway initiates biofilm formation in response to environmental stresses. the phosphotransferase protein vanu is the focal point of the quorum-sensing pathway and facilitates the regulation between independent phosphorelay systems that activate or repress biofilm formation. here we report the (1)h, (13)c, and (15)n ... | 2014 | 23604692 |
| identification of cathepsin b in the razor clam sinonovacula constricta and its role in innate immune responses. | cathepsin b, a lysosomal cysteine protease, has drawn much attention in vertebrates. however, very little is known about the functions of cathepsin b in bivalves. in this study, we identified the cathepsin b gene in the razor clam sinonovacula constricta. the protein has a typical cysteine protease structure, comprising a 15-residue putative signal peptide, a 75-residue propeptide and a 249-residue mature domain. in the mature domain, there is an occluding loop, an oxyanion hole (gln) and a cata ... | 2013 | 23632254 |
| a novel beta-defensin antimicrobial peptide in atlantic cod with stimulatory effect on phagocytic activity. | a novel defensin antimicrobial peptide gene was identified in atlantic cod, gadus morhua. this three exon/two intron defensin gene codes for a peptide precursor consisting of two domains: a signal peptide of 26 amino acids and a mature peptide of 40 residues. the mature cod defensin has six conserved cysteine residues that form 1-5, 2-4 and 3-6 disulphide bridges. this pattern is typical of beta-defensins and this gene was therefore named cod beta-defensin (defb). the tertiary structure of defb ... | 2013 | 23638029 |
| conserved structural complement component c3 in miiuy croaker miichthys miiuy and their involvement in pathogenic bacteria induced immunity. | complement component c3 is a key protein in the complement system whose activation is essential for all the important functions performed by this system. in this study, the complete c3 cdna sequence was isolated from the miiuy croaker (miichthys miiuy), which was high similarity to other complement c3. in this study, we report the primary sequence, the tissue expression profile, the polypeptide domain architecture and the phylogenetic analysis of miiuy croaker c3 gene. rapid amplification of the ... | 2013 | 23643875 |
| the response profiles of hspa12a and tctp from mytilus galloprovincialis to pathogen and cadmium challenge. | heat shock 70 kda protein 12a (hspa12a) is an atypical member of hsp70 family, and the translationally controlled tumor protein (tctp) is a novel hsp with chaperone-like activity. they are both involved in protecting organisms against various stressors. in the present study, the cdnas of hspa12a and tctp (called mghspa12a and mgtctp) were identified from mytilus galloprovincialis by race approaches. the full-length cdna of mghspa12a and mgtctp encoded a peptide of 491 and 171 amino acids, respec ... | 2013 | 23643947 |
| electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor. | an electrochemical disinfection system employing a honeycombed platinum coated titanium electrode was developed for the disinfection of seawater. cell suspensions (2 l, 10³ cells/ml) of the fish pathogens, vibrio alginolyticus, edwardsiella tarda, lactococcus garvieae and vibrio anguillarum were circulated in a reactor equipped with 10 sets of these electrodes at a flow rate of 200 ml/min with an applied potential of 1.0 v vs. ag/agcl reference electrode. the circulated cells were completely dis ... | 2013 | 23648105 |
| plasmid- and chromosome-encoded siderophore anguibactin systems found in marine vibrios: biosynthesis, transport and evolution. | vibrio anguillarum is a marine pathogen that causes vibriosis, a hemorrhagic septicemia in aquatic invertebrate as well as vertebrate animals. the siderophore anguibactin system is one of the most important virulence factors of this bacterium. most of the anguibactin biosynthesis and transport genes are located in the 65-kb pjm1 virulence plasmid although some of them are found in the chromosome of this fish pathogen. over 30 years of research unveiled the role numerous chromosomal and pjm1 gene ... | 2013 | 23660776 |
| a single whey acidic protein domain containing protein (swd) inhibits bacteria invasion and dissemination in shrimp marsupenaeus japonicus. | the single whey acidic protein (wap) domain containing proteins (swds) in crustacean belong to type iii crustins and have antiprotease activities and/or antimicrobial activities. their functions in vivo in crustacean immunity need to be clarify. in this study, a new single wap domain containing protein (swd) was obtained from marsupenaeus japonicus, designated as mjswd. the full-length cdna of mjswd was 522 bp.the open reading frame of mjswd encoded a protein of 79 amino acids, with a 24 amino a ... | 2013 | 23665548 |
| differentially expressed proteins in the skin mucus of atlantic cod (gadus morhua) upon natural infection with vibrio anguillarum. | vibriosis caused by v. anguillarum is a commonly encountered disease in atlantic cod farms and several studies indicate that the initiation of infection occurs after the attachment of the pathogen to the mucosal surfaces (gut, skin and gills) of fish. therefore it is necessary to investigate the role of different mucosal components in fish upon v. anguillarum infection. the present study has two parts; in the first part we analyzed the differential expression of skin mucus proteins from atlantic ... | 2013 | 23672475 |
| responses of mytilus galloprovincialis to bacterial challenges by metabolomics and proteomics. | pathogens can cause diseases and lead to massive mortalities of aquaculture animals and substantial economic loss. in this work, we studied the responses induced by micrococcus luteus and vibrio anguillarum in gill of mussel mytilus galloprovincialis at protein and metabolite levels. metabolic biomarkers (e.g., amino acids, betaine, atp) suggested that both m. luteus and v. anguillarum induced disturbances in energy metabolism and osmotic regulation. the unique and some more remarkably altered m ... | 2013 | 23711471 |
| cloning and identification of four mu-type glutathione s-transferases from the giant freshwater prawn macrobrachium rosenbergii. | glutathione s-transferases (gsts) are essential components of the cellular detoxification system because of their capability to protect organisms against the toxicity of reactive oxygen species (ross). four different gsts (mrmugst1-mrmugst4) showing similarities with mu-type gsts were cloned from the hepatopancreas of macrobrachium rosenbergii. these four gsts have 219, 216, 218 and 219 amino acids in length, respectively. mrmugst1-mrmugst4 proteins all have a g-site in the n-terminus and an h-s ... | 2013 | 23727284 |
| genetic analysis of vibriosis and viral nervous necrosis resistance in atlantic cod (gadus morhua l.) using a cure model. | the aim of this study was to investigate whether observed time-until-death of atlantic cod (gadus morhua l.) juveniles in separate challenge tests with vibrio anguillarum (causes vibriosis) and nodavirus [causes viral nervous necrosis (vnn)] are due to differences in susceptibility (whether at risk or not) or increased endurance (individual hazard, given that the animal is susceptible) using a cure mixture (cure) model with gibbs sampling. observed time-until-death, prepared as sequential binary ... | 2013 | 23736060 |
| molecular characterization and expression analysis of four cathepsin l genes in the razor clam, sinonovacula constricta. | cathepsin l (ctsl) is a lysosomal cysteine protease involved in immune responses in vertebrates. however, few studies exist regarding the role of cathepsin l in bivalves. in this study, we isolated and characterized four cathepsin l genes from the razor clam sinonovacula constricta, referred to as ctsl1, ctsl2, ctsl3 and ctsl4. these four genes contained typical papain-like cysteine protease structure and enzyme activity sites with erwnin-like and gnfd-like motifs in the proregion domain and an ... | 2013 | 23765116 |
| anguibactin- versus vanchrobactin-mediated iron uptake in vibrio anguillarum: evolution and ecology of a fish pathogen. | vibrio anguillarum is a marine bacterium that is present in many marine aquatic environments and that is the main cause of vibriosis in diverse wild and cultured fish species. two siderophore-mediated iron uptake systems have been described in v. anguillarum. one, mediated by the siderophore anguibactin, is encoded by the pjm1-type plasmids and is restricted to serotype o1 strains. the second one is mediated by the vanchrobactin siderophore and is widespread in many strains belonging to differen ... | 2010 | 23765994 |
| colonization of fish skin is vital for vibrio anguillarum to cause disease. | vibrio anguillarum causes a fatal haemorrhagic septicaemia in marine fish. during initial stages of infection, host surfaces are colonized; however, few virulence factors required for colonization of the host are identified. in this study, in vivo bioluminescent imaging was used to analyse directly the colonization of the whole rainbow trout animal by v. anguillarum. the wild type rapidly colonized both the skin and the intestines by 24 h; however, the bacterial numbers on the skin were signific ... | 2010 | 23766008 |
| immune responses evoked by infection with vibrio anguillarum in zebrafish bath-vaccinated with a live attenuated strain. | live attenuated vaccines are a promising application to control bacterial fish diseases. a live attenuated vibrio anguillarum vaccine candidate was established in our laboratory to protect fish against vibriosis. to elucidate the mechanism of immunoprotection, it is necessary to compare the different immune responses to infection between vaccinated and non-vaccinated fish. in this study, the expression levels of pathogen-specific antibodies and immune-related genes upon challenge at 28 days post ... | 2013 | 23768660 |
| expression profiles of selenium dependent glutathione peroxidase and glutathione s-transferase from exopalaemon carinicauda in response to vibrio anguillarum and wssv challenge. | a selenium-dependent glutathione peroxidase cdna was obtained from the ridgetail white prawn exopalaemon carinicauda (ecgpx) by rapid amplification of cdna ends (race) methods. the full-length cdna of ecgpx was 946 bp, which contains a 5'-untranslated region (utr) of 105 bp, 3'-utr of 280 bp with a poly (a) tail, an open reading frame (orf) of 561 bp, encoding a 186 amino-acid polypeptide with the predicted molecular weight of 21.35 kda and estimated isoelectric point of 7.65. it involves a puta ... | 2013 | 23770349 |
| two rab gtpases, esrab-1 and esrab-3, involved in anti-bacterial response of chinese mitten crab eriocheir sinensis. | rab gtpase is essential for the control of intracellular membrane trafficking in all eukaryotic cells and further affects the ability of phagocytic cells to scavenge pathogen. in the present study, the cdnas for two crab rab proteins (esrab-1 and esrab-3) were identified from the chinese mitten crab eriocheir sinensis by rapid amplification of cdna ends (race) approaches and expressed sequence tag (est) analysis. the full-length cdnas of esrab-1 and esrab-3 were of 892 bp and 965 bp with orfs of ... | 2013 | 23791860 |
| modulation of haemocyte phagocytic and antibacterial activity by alpha-adrenergic receptor in scallop chlamys farreri. | the adrenergic receptors are a class of g protein-coupled receptors, through which norepinephrine and epinephrine trigger the second messenger to modulate the immune response in immunocytes of vertebrate. in the present study, a gene coding the homologue of α-adrenergic receptor was identified from scallop chlamys farreri (designated cfαar). its deduced protein comprised 318 amino acids, and contained a conserved 7tm_1 domain. after cfαar protein was expressed in the hek293 cells, the stimulatio ... | 2013 | 23811406 |
| disruption of cell-to-cell signaling does not abolish the antagonism of phaeobacter gallaeciensis toward the fish pathogen vibrio anguillarum in algal systems. | quorum sensing (qs) regulates phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that qs is not important for antagonism in algal cultures. qs mutants reduced vibrio anguillarum to the same extent as the wild type. consequently, a combination of probiotic phaeobacter and qs inhibitors is a feasible strategy for aquaculture disease control. | 2013 | 23811510 |
| characterization of the ccr3 and ccr9 genes in miiuy croaker and different selection pressures imposed on different domains between mammals and teleosts. | the innate immune system can recognize non-self through pattern recognition receptors and provides a first line of antimicrobial host defense. thus innate immunity plays a very important role in resistance against major bacterial disease in vertebrates. in the innate immune responses, the chemokine receptors act as the main receptors of chemokines which are released at the sites of infection, inflammation and injury. in this study, the miichthys miiuy ccr3 and ccr9 genes were cloned and characte ... | 2013 | 23817141 |
| a novel c-type lysozyme from mytilus galloprovincialis: insight into innate immunity and molecular evolution of invertebrate c-type lysozymes. | a c-type lysozyme (named as mgclyz) gene was cloned from the mussel mytilus galloprovincialis. blast analysis indicated that mgclyz was a salivary c-type lysozyme which was mainly found in insects. the nucleotide sequence of mgclyz was predicted to encode a polypeptide of 154 amino acid residues with the signal peptide comprising the first 24 residues. the deduced mature peptide of mgclyz was of a calculated molecular weight of 14.4 kd and a theoretical isoelectric point (pi) of 8.08. evolution ... | 2013 | 23818979 |
| h-ns is a negative regulator of the two hemolysin/cytotoxin gene clusters in vibrio anguillarum. | hemolysins produced by vibrio anguillarum have been implicated in the development of hemorrhagic septicemia during vibriosis, a fatal fish disease. previously, two hemolysin gene clusters responsible for the hemolysis and cytotoxicity of v. anguillarum were identified: the vah1-plp gene cluster and the rtxachbde gene cluster. in this study, we identified the hns gene, which encodes the h-ns protein and acts as a negative regulator of both gene clusters. the v. anguillarum h-ns protein shares str ... | 2013 | 23836825 |
| concholepas concholepas ferritin h-like subunit (ccfer): molecular characterization and single nucleotide polymorphism associated to innate immune response. | ferritin has been identified as the principal protein of iron storage and iron detoxification, playing a pivotal role for the cellular homeostasis in living organisms. however, recent studies in marine invertebrates have suggested its association with innate immune system. in the present study, one ferritin subunit was identified from the gastropod concholepas concholepas (ccfer), which was fully characterized by rapid amplification of cdna ends technique. simultaneously, a challenge test was pe ... | 2013 | 23838046 |
| characterization of an immune deficiency homolog (imd) in shrimp (fenneropenaeus chinensis) and crayfish (procambarus clarkii). | the immune deficiency (imd) signal pathway mediates immunity against gram-negative bacteria in drosophila. recent studies show that the imd pathway also involves in antiviral innate immune responses. the functions of the pathway in crustacean immunity are largely unknown. in this paper, two imds (fcimd and pcimd), one of the key elements of the imd pathway, were identified from chinese white shrimp fenneropenaeus chinensis and red swamp crayfish procambarus clarkii. both proteins have a death do ... | 2013 | 23850721 |
| molecular characterization of hepcidin gene in common carp (cyprinus carpio l.) and its expression pattern responding to bacterial challenge. | hepcidin is a cysteine-rich cationic antimicrobial peptide (amp), which plays an important role in host innate immune system and iron regulation. a great many of hepcidin genes have been identified from vertebrates, including various fish species. here we report the cloning and identification of a hepcidin cdna from the liver of common carp (cyprinus carpio l.). the full-length cdna of the common carp hepcidin was 647 bp, which contained an orf of 276 bp encoding a prepropeptide of 91 amino acid ... | 2013 | 23851290 |
| functional analysis of domain of unknown function (duf) 1943, duf1944 and von willebrand factor type d domain (vwd) in vitellogenin2 in zebrafish. | vitellogenin (vg), the precursor of egg-yolk proteins in all oviparous organisms, shares a similar domain structure combination. in most cases, vg contains the vitellogenin_n domain, the domain of unknown function (duf) 1943, and the von willebrand factor type d domain (vwd), which are present in different forms of vg from both vertebrates and invertebrates. occasionally, a duf1944 domain is also present in between duf1943 and vwd in some vg proteins of vertebrates. recent studies have shown tha ... | 2013 | 23867754 |
| molecular characterization and expression analysis of toll-like receptor 21 cdna from paralichthys olivaceus. | toll-like receptor (tlr) is believed to play crucial role in host defense of pathogenic microbes in innate immune system. in the present study, the full-length cdna of paralichthys olivaceus toll-like receptor 21 (po-tlr21) was cloned by homology cloning and rapid amplification of cdna ends (race) technique. the po-tlr21 cdna sequence was 3687 bp, containing an open reading frame of 2922 bp encoding 973 amino acids. tmhmm and smart program analysis indicated that protein contained one transmembr ... | 2013 | 23880453 |
| differential response of two ferritin subunit genes (vpfer1 and vpfer2) from venerupis philippinarum following pathogen and heavy metals challenge. | as a principal extracellular iron storage molecule, ferritin plays an important role in the iron-withholding strategy of innate immunity and detoxification system. in this study, we cloned and characterized another ferritin from venerupis philippinarum (designated as vpfer2), in addition to one previously reported (vpfer1). vpfer2 possessed all the conserved features critical for the fundamental structure and function of ferritin h subunit. vpfer1 and vpfer2 mrna were both found to be most abund ... | 2013 | 23891591 |
| effectiveness of probiotic phaeobacter bacteria grown in biofilters against vibrio anguillarum infections in the rearing of turbot (psetta maxima) larvae. | the rearing environment of first-feeding turbot larvae, usually with high larvae densities and organic matter concentrations, may promote the growth of opportunistic pathogenic vibrionaceae bacteria, compromising the survival of the larvae. the aim of this study was to assess the effectiveness of the biofilm-forming probiotic phaeobacter 27-4 strain grown on a ceramic biofilter (probiofilter) in preventing vibrio anguillarum infections in turbot larvae. in seawater with added microalgae and main ... | 2013 | 23917459 |
| recombinant tnfα as oral vaccine adjuvant protects european sea bass against vibriosis: insights into the role of the ccl25/ccr9 axis. | vibrio anguillarum is the main causative agent of vibriosis in cultured sea bass. unfortunately, available vaccines against this disease do not achieve the desired protection. in this study, to accomplish uptake, processing, and presentation of luminal antigens, a commercial sea bass oral vaccine against v. anguillarum was improved with the addition of recombinant fish-self tumor necrosis factor α (rtnfα), as adjuvant. to explore mechanisms, systemic and local responses were analyzed through ser ... | 2013 | 23932985 |
| the involvement of pdgf/vegf related factor in regulation of immune and neuroendocrine in chinese mitten crab eriocheir sinensis. | members of the platelet-derived growth factor/vascular endothelial growth factor (pdgf/vegf) family have been implicated in cell proliferation, cell differentiation, and cell migration, vascular development, angiogenesis and neural development. in the present study, a novel pdgf/vegf related factor gene was cloned and identified in chinese mitten crab eriocheir sinensis (designated as espvf1). the full-length cdna of espvf1 was of 1173 bp, consisting a 5' untranslated region (utr) of 54 bp, a 3' ... | 2013 | 23933264 |
| molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (stat2) from turbot, scophthalmus maximus. | signal transducer and activator of transcription 2 (stat2) is an important molecule involved in the type i interferon signalling pathway. to date, little stat2 homologue is available in fish except atlantic salmon and goldfish. in this paper, stat2 was firstly cloned and characterized from turbot, a marine flatfish with high economic value. briefly, turbot stat2 cdna is 3206 bp in length encoding a predicted protein of 793 amino acids. the phylogenetic tree shows that turbot stat2 protein shared ... | 2013 | 23933433 |
| does virulence assessment of vibrio anguillarum using sea bass (dicentrarchus labrax) larvae correspond with genotypic and phenotypic characterization? | vibriosis is one of the most ubiquitous fish diseases caused by bacteria belonging to the genus vibrio such as vibrio (listonella) anguillarum. despite a lot of research efforts, the virulence factors and mechanism of v. anguillarum are still insufficiently known, in part because of the lack of standardized virulence assays. | 2013 | 23936439 |
| identification and functional studies of akirin, a potential positive nuclear factor of nf-κb signaling pathways in the pacific white shrimp, litopenaeus vannamei. | as conserved nuclear factors, akirins play critical roles in regulating antimicrobial peptides (amps) transcription downstream of nf-κb dependent signaling pathways in insects and mammals. however, no any functional studies was reported in penaeid shrimp. the identification and functional analysis of akirin in the pacific white shrimp, litopenaeus vannamei were made in this research. the 833 nucleotides cdna of litopenaeus vannamei akirin (lvakirin) was obtained with an open reading frame of 639 ... | 2013 | 23962743 |
| a c-type lectin could selectively facilitate bacteria clearance in red swamp crayfish, procambarus clarkii. | c-type lectins function as pattern recognition receptors and play important roles in the innate immune system of crustaceans. in this study, we reported a new ctl gene (designated as pclec5) from red swamp crayfish, procambarus clarkii. pclec5 was mainly distributed in hepatopancreas, gills and intestine, and the pclec5 transcripts were up-regulated in all the three tissues after challenge with bacteria vibrio anguillarum. for further functional analyses, pclec5 was recombinantly expressed in es ... | 2013 | 23989040 |
| molecular cloning and transcriptional regulation of an allograft inflammatory factor-1 (aif-1) in zhikong scallop chlamys farreri. | the allograft inflammatory factor-1 (aif-1) is one of the key factors associated with inflammatory response. in the present study, the full-length cdna of aif-1 was identified from zhikong scallop chlamys farreri (named as cfaif-1) by est (expressed sequence tag) analysis and race (rapid-amplification of cdna ends) approaches. the cdna of cfaif-1 consisted of a 5-terminal untranslated region (utr) of 58 bp, a 3-utr of 607 bp with a poly (a) tail, and an open reading frame (orf) of 468 bp encodin ... | 2013 | 24001778 |
| a c-type lectin (aictl-3) from bay scallop argopecten irradians with mannose/galactose binding ability to bind various bacteria. | c-type lectins are a family of ca(2+)-dependent carbohydrate-binding proteins playing crucial roles in innate immunity of vertebrates and invertebrates. in the present study, the cdna of a c-type lectin with one carbohydrate-recognition domain (crd) of 127 amino acids was cloned from bay scallop argopecten irradians (designated aictl-3) by rapid amplification of cdna end (race) techniques based on expressed sequence tag (est) analysis. the mrna transcripts of aictl-3 could be detected in all the ... | 2013 | 24008017 |
| clinical vibrio anguillarum infection in lumpsucker cyclopterus lumpus in scotland. | 2013 | 24008994 | |
| transcriptome profiling reveals th17-like immune responses induced in zebrafish bath-vaccinated with a live attenuated vibrio anguillarum. | a candidate vaccine, live attenuated vibrio anguillarum developed in our laboratory could prevent vibriosis of fish resulted from v. anguillarum and v. alginolyticus. to elucidate the molecular mechanisms underlying the vaccine protection, we used microarray technology to compare the spleen transcriptomes of bath-vaccinated and unvaccinated zebrafish at 28 days post vaccination. | 2013 | 24023910 |
| application of relative real-time pcr to detect differential expression of virulence genes in vibrio anguillarum under standard and stressed growth conditions. | in this study, we aimed to understand whether abiotic factors affect the expression of virulence genes in vibrio anguillarum. we observed the in vitro responses of two mediterranean strains of v. anguillarum to temperature, nacl and iron concentration changes. we monitored growth performance and gene transcription levels by comparing the results obtained under stressed conditions (temperatures of 5 °c, 15 °c and 37 °c; nacl concentrations of 3% and 5%; and iron depletion and excess) with those o ... | 2014 | 24033758 |
| vibrio anguillarum bacterin uptake via the gills of japanese flounder and subsequent immune responses. | the mucosal surfaces of fish allow for the introduction of foreign substances, including antigens, from the surrounding environment. in this study, uptake of vibrio anguillarum j-o-3 serotype bacterin by japanese flounder, and the subsequent immune responses were investigated. immunohistochemistry revealed that the bacterin was taken up through the epithelial cells of gills. the transcription levels of inflammatory cytokines such as interleukin (il)-1β, il-6 and tumor necrosis factor α were sign ... | 2013 | 24035752 |
| proteomic and metabolomic responses in hepatopancreas of mytilus galloprovincialis challenged by micrococcus luteus and vibrio anguillarum. | the outbreak of pathogens can induce diseases and lead to massive mortalities of aquaculture animals including fish, mollusk and shrimp. in this work, the responses induced by micrococcus luteus and vibrio anguillarum were investigated in hepatopancreas of mussel mytilus galloprovincialis using proteomics and metabolomics. metabolic biomarkers demonstrated that m. luteus and v. anguillarum injections could induce osmotic stress and disturbance in energy metabolism. and the uniquely and more mark ... | 2013 | 24056185 |
| differential metabolic responses of clam ruditapes philippinarum to vibrio anguillarum and vibrio splendidus challenges. | clam ruditapes philippinarum is one of the important marine aquaculture species in north china. however, pathogens can often cause diseases and lead to massive mortalities and economic losses of clam. in this work, we compared the metabolic responses induced by vibrio anguillarum and vibrio splendidus challenges towards hepatopancreas of clam using nmr-based metabolomics. metabolic responses suggested that both v. anguillarum and v. splendidus induced disturbances in energy metabolism and osmoti ... | 2013 | 24056279 |
| comparison of protein expression profiles of the hepatopancreas in fenneropenaeus chinensis challenged with heat-inactivated vibrio anguillarum and white spot syndrome virus. | fenneropenaeus chinensis (chinese shrimp) culture industry, like other penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. to better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. firstly, the soluble hepatopancreas protein samples in adult chinese shrimp among control, heat-inactivated vibrio-challenged and white spot syndrome virus-infected groups were separated ... | 2014 | 24057166 |
| complete genome sequence of vibrio anguillarum m3, a serotype o1 strain isolated from japanese flounder in china. | vibrio anguillarum is an important bacterial pathogen that causes vibriosis in marine fish. we present the complete genome sequence of v. anguillarum m3, a serotype o1 clinical strain isolated from japanese flounder (paralichthys olivaceus) in shandong, china. | 2013 | 24072867 |
| production of homozygous transgenic rainbow trout with enhanced disease resistance. | previous studies conducted in our laboratory showed that transgenic medaka expressing cecropin b transgenes exhibited resistant characteristic to fish bacterial pathogens, pseudomonas fluorescens and vibrio anguillarum. to confirm whether antimicrobial peptide gene will also exhibit anti-bacterial and anti-viral characteristics in aquaculture important fish species, we produced transgenic rainbow trout expressing cecropin p1 or a synthetic cecropin b analog, cf-17, transgene by sperm-mediated ge ... | 2013 | 24085608 |
| a fibrinogen-related protein (tffrep2) gene involving in the immune response of trachidermus fasciatus against vibrio anguillarum. | fibrinogen-related proteins play important roles in the immune responses. we have obtained a cdna encoding a novel fibrinogen-related protein from roughskin sculpin trachidermus fasciatus (t. fasciatus) and named it as tffrep2. the n and c terminus of tffrep2 contain a putative 21-amino acid signal peptide and a typical 217-amino acid fibrinogen-like domain, which is conserved in all fibrinogen-related proteins. tffrep2 has three glycosylation sites and two potential calcium-binding sites that a ... | 2013 | 24091062 |
| effect of multiple infections with white spot syndrome virus and vibrio anguillarum on pacific white shrimp litopenaeus vannamei (l.): mortality and viral replication. | multiple infections are commonly found in practical shrimp culture and may cause more serious consequences than infections by one pathogen only. therefore, this study was conducted to evaluate the effect of multiple infections with white spot syndrome virus (wssv) and vibrio anguillarum on pacific white shrimp litopenaeus vannamei (l.), mortality, wssv replication in vivo and host immune response. in the wssv single-infection group (wssv load, 2 × 10(2) copies μl(-1)), mean cumulative mortality ... | 2014 | 24127689 |
| antibacterial activity of the lipopetides produced by bacillus amyloliquefaciens m1 against multidrug-resistant vibrio spp. isolated from diseased marine animals. | in this work, the antibacterial activity of the lipopeptides produced by bacillus amyloliquefaciens m1 was examined against multidrug-resistant vibrio spp. and shewanella aquimarina isolated from diseased marine animals. a new and cheap medium which contained 1.0 % soybean powder, 1.5 % wheat flour, ph 7.0 was developed. a crude surfactant concentration of 0.28 mg/ml was obtained after 18 h of 10-l fermentation and diameter of the clear zone on the plate seeded with vibrio anguillarum was 34 mm. ... | 2014 | 24132666 |
| a farnesoic acid o-methyltransferase (famet) from exopalaemon carinicauda is responsive to vibrio anguillarum and wssv challenge. | methyl farnesoate (mf), an analogue of the insect juvenile hormone iii, is believed to play important roles in the regulation of the growth and reproductive development in crustaceans. farnesoic acid o-methyltransferase (famet) is the key enzyme in the juvenile hormone biosynthetic pathway, involved in the conversion of farnesoic acid (fa) to mf in the final step of mf synthesis. in this study, a famet cdna (named ecfamet) was cloned from the hemocytes of ridgetail white prawn exopalaemon carini ... | 2014 | 24136172 |
| oral administration of formalin killed vibrio anguillarum cells improves growth and protection against challenge with vibrio harveyi in banana shrimp. | larval rearing in hatcheries and highly intensive grow-out culture practices followed in shrimp production systems favour the growth of potential pathogenic bacterial loads. this study reports the efficacy of formalin-killed vibrio bacterin on growth, survival and protection to challenge with virulent vibrio harveyi and vibrio anguillarum in juveniles of banana shrimp fenneropenaeus merguiensis. postlarvae 15 (0·24 ± 0·01 g) were administered orally in different concentrations of bacterial prepa ... | 2014 | 24138117 |
| cdna cloning, characterization and expression analysis of peroxiredoxin 5 gene in the ridgetail white prawn exopalaemon carinicauda. | peroxiredoxin is a superfamily of antioxidative proteins that play important roles in protecting organisms against the toxicity of reactive oxygen species. in this study, a full-length of peroxiredoxin 5 (designated ecprx5) cdna was cloned from the ridgetail white prawn exopalaemon carinicauda by using rapid amplification of cdna ends (race) approaches. the full-length cdna of the ecprx5 was of 827 bp, containing a 5' untranslated region (utr) of 14 bp, a 3' utr of 228 bp with a poly (a) tail, a ... | 2013 | 24141991 |
| immune-related functions of the hivep gene family in east african cichlid fishes. | immune-related genes are often characterized by adaptive protein evolution. selection on immune genes can be particularly strong when hosts encounter novel parasites, for instance, after the colonization of a new habitat or upon the exploitation of vacant ecological niches in an adaptive radiation. we examined a set of new candidate immune genes in east african cichlid fishes. more specifically, we studied the signatures of selection in five paralogs of the human immunodeficiency virus type i en ... | 2013 | 24142922 |
| probiotics-pathogen interactions elicit differential regulation of cutaneous immune responses in epidermal cells of atlantic cod gadus morhua. | little is known on the cutaneous immune responses during probiotics-pathogen interactions in fish. thus, this study employed atlantic cod primary epidermal (ep) cell cultures as a model to understand this interaction. the probiotics-pathogen interactions in the ep cell cultures were elucidated using vibrio anguillarum 2133 (va) as the pathogen and two host-derived bacteria (gp21 and gp12) as the probiotics. there was a regional size difference on the ep cells; i.e., ep cells from the dorsal regi ... | 2014 | 24176817 |
| the detection of fish pathogenvibrio anguillarum in water and fish using a species-specific dna probe combined with membrane filtration. | the marine bacteriumvibrio anguillarum causes disease in fish worldwide and is particularly devastating in aquaculture. little is known about the ecology ofv. anguillarum in the environment and how this may relate to the pathogenicity of this organism. combining membrane filtration and a species-specific dna probe, culturablev. anguillarum cells were detected in water from three habitats and in chinook salmon (onchorynchus tshawytscha) tissue samples. results show that different marine habitats ... | 1994 | 24186556 |
| molecular responses of calreticulin gene to vibrio anguillarum and wssv challenge in the ridgetail white prawn exopalaemon carinicauda. | calreticulin (crt), as a highly conserved endoplasmic reticulum luminal resident protein, plays important roles in ca(2+) homeostasis, molecular chaperoning and response to viral infection. in this study, a full-length cdna of crt (designated eccrt) was cloned from hemocytes of the ridgetail white prawn exopalaemon carinicauda by using rapid amplification of cdna ends (race) approaches. the full-length cdna of eccrt was 1725 bp, which contains a 5'-untranslated region (utr) of 57 bp, 3'-utr of 4 ... | 2014 | 24188748 |
| involvement of pacific oyster cgpgrp-s1s in bacterial recognition, agglutination and granulocyte degranulation. | peptidoglycan recognition protein (pgrp) recognizes invading bacteria through their peptidoglycans (pgn), a component of the bacterial cell wall. insect pgrps contribute to effective immune systems as inducers of other host defense responses, while this function has not been reported from pgrp of bivalves. in this study, recombinant cgpgrp-s1s (rcgpgrp-s1s), produced in the mantle and the gill, was synthesized and used to elucidate the immunological function of cgpgrp-s1s. rcgpgrp-s1s bound spec ... | 2014 | 24201133 |
| ecvig, a novel grouper immune-gene associated with antiviral activity against nnv infection. | vhsv-induced genes (vigs) were first identified in rainbow trout (oncorhynchus mykiss) and subsequently isolated in a variety of fish. recent studies have shown that most vigs have immunological functions against pathogenic infections. however, most research has focused on vig1, such that our present understanding of these genes in other fish species remains limited. this study isolated a homologue of the uncharacterized o. mykiss vig-b319 (ecvig) from orange-spotted grouper (epinephelus coioide ... | 2014 | 24211340 |
| identification, characterization and functional analysis of a serine protease inhibitor (lvserpin) from the pacific white shrimp, litopenaeus vannamei. | as important arthropod immune responses, prophenoloxidase (propo) activation and toll pathway initiation are mediated by serine proteinase cascades and regulated by serpins. herein, a serine protease inhibitor (lvserpin), encoding for 415 amino acids with calculated molecular weight of 46,639 da and isoelectric point of 7.03 was characterized from the pacific white shrimp litopenaeus vannamei. multiple sequence alignment revealed that lvserpin shared the highest similarity with penaeus monodon s ... | 2014 | 24211341 |