Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| brucellosis in the etiology of febrile neutropenia: case report. | brucellosis is one of the leading diseases in the differential diagnosis of fever of unknown origin in some parts of the world. it can lead to treatment failure because of slow growth in blood cultures and late appearance of signs and symptoms in patients with febrile neutropenia who were unresponsive to empirical antibiotic treatment. during the last year in our oncology unit adjuvant chemotherapy was given to 3 patients with breast (n=1) and stomach cancer (n=2) and febrile neutropenia was see ... | 2002 | 11892906 |
| splenic infarction associated with acute brucella mellitensis infection. | 2002 | 11913506 | |
| identification of the brucella melitensis vaccine strain rev.1 in animals and humans in israel by pcr analysis of the psti site polymorphism of its omp2 gene. | adverse effects of strain persistence and secretion in milk have been encountered with the brucella melitensis vaccine strain rev.1. field isolates obtained from vaccinated animals and from a human resembled the vaccine strain rev.1 by conventional bacteriological tests. the lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirmation of the homology of the rev.1-like field isolates to the vaccine strain. the composition of the omp ... | 2002 | 11923376 |
| medically treated splenic abscess due to brucella melitensis. | brucellosis may lead to complications that affect different organ systems, including the liver and spleen. in acute disease, hepatosplenic abscess is a rare complication. we report herein a woman with splenic abscess due to acute brucella melitensis infection who was successfully treated with antibiotics alone. | 2002 | 11928846 |
| chronic hepatosplenic abscesses in brucellosis. clinico-therapeutic features and molecular diagnostic approach. | in order to analyze the clinical and therapeutic features of chronic hepatosplenic abscesses, and to define the diagnostic yield of new molecular techniques, we describe seven cases, four hepatic and three splenic, of this uncommon complication of brucellosis. onset of symptoms in all cases was insidious and the diagnostic delay considerable. abdominal ct scan showed large, poorly defined lesions, with heterogeneous attenuation and thick central calcifications surrounded by hypointense areas. hi ... | 2002 | 11929686 |
| combined brucella melitensis and streptococcal viridans endocarditis. | infectious endocarditis is a potentially lethal inflammation of the hearts' inner lining invaded by microorganisms. the mortality from this illness increases as the number of infective organisms rises to 2, due to involvement of the left side of the heart. these microorganisms usually arise from the patient's own flora but can be acquired from the environment. fever and heart murmurs are the principal clinical manifestations followed by a plethora of peripheral signs due to dissemination of micr ... | 2002 | 11938375 |
| brucellosis as a cause of septic loosening of total hip arthroplasty. | infection after a total hip arthroplasty is a severe complication and is associated with a high incidence of morbidity. we describe a case of late infection, 5 years after the implantation of a cemented hip prosthesis. the infection was caused by gram-negative brucella melitensis and occurred in a 63-year-old man who owned cattle. as far as we know, such a complication has never been published before. | 2002 | 11938520 |
| [analysis of immune response of guinea pigs infected with brucella melitensis]. | the dynamics of the first antigen specific stage of immune response to brucella infection was experimentally studied with the method of binding adsorbed antigenic immunoreagents with lymphocytes. the study revealed that the content of antigen-binding lymphocytes (abl) reached its maximum as early as on day 7 after infection, gradually decreasing afterwards (but even on day 90 abl could be detected in the blood). the specificity of abl was proved by the fact that they were absent in noninfected a ... | 2002 | 11949255 |
| pulmonary infection due to brucella melitensis. | 2001 | 11953226 | |
| spinal brucellosis: case report in the united states. | case report. | 2002 | 11979184 |
| [diagnosis of human brucellosis. role of ph in the seroagglutination test and influence of ph on the agglutinating activity of igm, igg and iga antibodies]. | to evaluate the role of ph in the seroagglutination test (sat)and rose bengal (rb) test, and to determine the influence of ph on the agglutinating activity of igm, igg and iga antibodies. | 2002 | 11996698 |
| identification of a quorum-sensing signal molecule in the facultative intracellular pathogen brucella melitensis. | brucella melitensis is a gram-negative alpha2-proteobacterium responsible for abortion in goats and for malta fever in humans. this facultative intracellular pathogen invades and survives within both professional and nonprofessional phagocytes. a dichloromethane extract of spent culture supernatant from b. melitensis induces bioluminescence in an escherichia coli acyl-homoserine lactone (acyl-hsl) biosensor strain based upon the activity of the lasr protein of pseudomonas aeruginosa. hplc fracti ... | 2002 | 12010991 |
| expression cloning and characterization of the c28 acyltransferase of lipid a biosynthesis in rhizobium leguminosarum. | an unusual feature of lipid a from plant endosymbionts of the rhizobiaceae family is the presence of a 27-hydroxyoctacosanoic acid (c28) moiety. an enzyme that incorporates this acyl chain is present in extracts of rhizobium leguminosarum, rhizobium etli, and sinorhizobium meliloti but not escherichia coli. the enzyme transfers 27-hydroxyoctacosanate from a specialized acyl carrier protein (acpxl) to the precursor kdo2 ((3-deoxy-d-manno-octulosonic acid)2)-lipid iv(a). we now report the identifi ... | 2002 | 12019272 |
| brucella peritonitis in a patient on continuous ambulatory peritoneal dialysis with acute brucellosis. | peritonitis is an uncommon complication of brucellosis. brucella peritonitis in chronic ambulatory peritoneal dialysis (capd) patients has not been reported before. a male patient is presented with peritonitis caused by brucella melitensis who was on capd. the source of infection was thought to be unpasteurized, unsalted cheese eaten a month before the onset of symptoms. at the beginning, antibiotic therapy with doxycyline and rifampicin led to a rapid clinical improvement, with disappearance of ... | 2002 | 12021533 |
| in vitro activities of new quinolones against brucella melitensis isolated in a tertiary-care hospital in turkey. | we have evaluated the in vitro activities of seven fluoroquinolones against 69 strains of brucella melitensis. according to their minimum inhibitory concentration for 90% growth (mic(90)) values, the most active agent was found to be sparfloxacin (mic(90) 0.12 mg/l) followed by levofloxacin, ciprofloxacin, ofloxacin (mic(90) 0.50 mg/l) and grepafloxacin (mic(90) 1 mg/l), gemifloxacin (mic(90) 2 mg/l) and gatifloxacin (mic(90) 4 mg/l). | 2002 | 12047416 |
| clinical microbiological case: a 22-year-old-man with fever and maculopapular rash. | 2002 | 12047418 | |
| identification of brucella melitensis vaccine strain rev.1 by pcr-rflp based on a mutation in the rpsl gene. | the live attenuated strain b. melitensis rev.1 is considered the best vaccine available for the prophylaxis of brucellosis in sheep and goats. the rev.1 vaccine was obtained in the 1950s by a two-step selection involving firstly streptomycin resistance and dependence and secondly reversion of dependence but keeping streptomycin resistance. chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding the ribosomal protein s12, rpsl. nucleotide sequencing reve ... | 2002 | 12057611 |
| protection of mice against brucellosis by intranasal immunization with brucella melitensis lipopolysaccharide as a noncovalent complex with neisseria meningitidis group b outer membrane protein. | intranasal immunization of mice with purified brucella melitensis lipopolysaccharide (lps) as a noncovalent complex with neisseria meningitidis group b outer membrane protein (gbomp) elicited a high-titer anti-lps systemic antibody response and a significant mucosal antibody response. the anti-lps immunoglobulin g (igg) antibody was predominantly of the igg1 subtype, although there was some response of the igg2a, igg2b, and igg3 subtypes. the antibody titer remained high for 16 weeks postimmuniz ... | 2002 | 12065469 |
| pathogenicity and protective activity in pregnant goats of a brucella melitensis deltaomp25 deletion mutant. | the brucella melitensis mutant bm 25, which lacks the major 25 kda outer membrane protein omp25, has previously been found to be attenuated in the murine brucellosis model. in the present study, the capacity of the deltaomp25 mutant to colonise and cause abortions in the caprine host was evaluated. the vaccine potential of bm 25 was also investigated in goats. inoculation of nine pregnant goats in late gestation with the b. melitensis mutant resulted in 0/9 abortions, while the virulent parental ... | 2002 | 12076120 |
| bacteria-modified amperometric immunosensor for a brucella melitensis antibody assay. | a novel amperometric immunosensor setup is described which uses horseradish peroxidase (hrp) as a label in conjunction with a current-based brucella sensor. the bacteria modified immunosensor was constructed by using a biocomposite formed by dispersing graphite powder into a mixture of brucella melitensis and silicate polymer gel. the enzyme-labeled antibody can readily diffuse toward the encapsulated antigen (brucella melitensis), which retains its binding properties, and the association reacti ... | 2002 | 12083544 |
| branchial cyst empyema due to brucella melitensis infection as a form of focal brucellosis. | 2002 | 12099739 | |
| leukocyte count in the synovial fluid of children with culture-proven brucellar arthritis. | brucellosis is an important cause of paediatric septic arthritis in endemic areas. because the gram stain is frequently negative and culture results are unavailable at the time of the patient's admission, the diagnosis of brucellar arthritis is usually entertained on the bases of epidemiological considerations and cytological examination of the synovial fluid aspirate. the aim of this study was to assess the sensitivity of a synovial fluid leukocyte count >50 000 wbc/mm(3) for detecting culture- ... | 2002 | 12111621 |
| fever of unknown origin in a previously healthy child. | 2002 | 12118845 | |
| comparison between immune responses and resistance induced in balb/c mice vaccinated with rb51 and rev. 1 vaccines and challenged with brucella melitensis bv. 3. | balb/c mice were immunized with live rough brucella abortus rb51 or smooth brucella melitensis rev. 1 vaccines and challenged with a b. melitensis field strain. protection was assessed by a variety of serological tests and recovery of vaccinal and challenge strains by culture. mice vaccinated with rb51 gave negative results in the conventional serological tests prior to challenge, namely; standard tube agglutination test (sat), rose bengal plate test (rbpt), buffered acidified plate antigen test ... | 2002 | 12119140 |
| brucellar orchitis in innerwest anatolia region of turkey. a report of 12 cases. | brucellosis, which affects the genitourinary system in rate of 2-20%, is a multiorgan infectious disease. twelve patients were diagnosed as having brucellar orchitis serologically, clinically and ultrasonographically. in 2 patients, brucella melitensis was isolated in blood cultures. all the patients were working in cattle dealing. they were treated with 600 mg/day rifampicin plus 200 mg/day doxycycline for 6 weeks and followed up during 1 year. they recovered clinically within 3 weeks. although ... | 2002 | 12119436 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 22-2002. a 37-year-old man with unexplained fever after a long trip through south america. | 2002 | 12124410 | |
| brucella species lacking the major outer membrane protein omp25 are attenuated in mice and protect against brucella melitensis and brucella ovis. | to aid in the development of novel efficacious vaccines against brucellosis, omp25 was examined as a potential candidate. to determine the role of omp25 in virulence, mutants were created with brucella abortus (ba25), brucella melitensis (bm25), and brucella ovis (bo25) which contain disruptions in the omp25 gene (deltaomp25 mutants). western immunoblot analysis and pcr verified that the omp25 protein was not expressed and that the omp25 gene was disrupted in each strain. balb/c mice infected wi ... | 2002 | 12151196 |
| brucella sp. bind to sialic acid residues on human and animal red blood cells. | we report that brucella abortus and brucella melitensis agglutinate human (a+ and b+), hamster and rabbit erythrocytes, a heretofore undescribed feature in this genus. this activity was associated with a 29-kda surface protein (sp29) that bound selectively to these erythrocytes and this binding was inhibited by rabbit anti-sp29 antibodies. hemagglutination was inhibited by pretreatment of erythrocytes with neuraminidase and by preincubation of b. abortus with chondroitin sulfate, n-acetylneurami ... | 2002 | 12167541 |
| antigen-specific activation and proliferation of cd4+ and cd8+ t lymphocytes from brucellosis patients. | salt-extractable antigen from brucella melitensis 16m (rcm-bm) was used to evaluate the immune response from acute and chronic patients suffering from brucella infections (in mexico); their responses were compared with those of healthy controls. as a readout we used upregulation of cd69 (a well-established early activation marker for lymphocytes), lymphocyte proliferation by 3[h]thymidine or 5-bromo-2-deoxyuridine (brdu) incorporation measured by liquid scintillation or flow cytometry, respectiv ... | 2002 | 12174793 |
| the yersinia pseudotuberculosis yut protein, a new type of urea transporter homologous to eukaryotic channels and functionally interchangeable in vitro with the helicobacter pylori urei protein. | urea uptake in eukaryotes and prokaryotes occurs via diffusion or active transport across the cell membrane. facilitated diffusion of urea in both types of organisms requires a single-component channel. in bacteria, these transport systems allow rapid access of urease to its substrate, resulting in ammonia production, which is needed either for resistance to acidity or as a nitrogen source. in yersinia pseudotuberculosis, a ureolytic enteropathogenic bacterium, a gene of unknown function (yut) l ... | 2002 | 12180933 |
| functional characterization of brucella melitensis normi, an efflux pump belonging to the multidrug and toxic compound extrusion family. | two putative proteins (normi and normii) similar to the multidrug efflux protein norm of vibrio parahaemolyticus are encoded by the brucella melitensis 16 m genome. we show that a drug-hypersusceptible escherichia coli strain overexpressing normi displays increased resistance to norfloxacin, ciprofloxacin, gentamicin, tetraphenylphosphonium ion, acriflavine, and berberine. this elevated resistance was proven to be mediated by an energy-dependent efflux mechanism. normi belongs to the multidrug a ... | 2002 | 12183269 |
| comparative proteome analysis of brucella melitensis vaccine strain rev 1 and a virulent strain, 16m. | the genus brucella consists of bacterial pathogens that cause brucellosis, a major zoonotic disease characterized by undulant fever and neurological disorders in humans. among the different brucella species, brucella melitensis is considered the most virulent. despite successful use in animals, the vaccine strains remain infectious for humans. to understand the mechanism of virulence in b. melitensis, the proteome of vaccine strain rev 1 was analyzed by two-dimensional gel electrophoresis and co ... | 2002 | 12193611 |
| isolated cervical lymphadenopathy as unique manifestation of brucellosis. | we report the case of a 42-y-old male with an isolated cervical lymphadenopathy due to brucella melitensis. the diagnosis was established by isolation of b. melitensis in a lymphatic specimen obtained by fine-needle aspiration and confirmed by serological test results showing high levels of brucella agglutinins. mycobacteria-specific cultures were negative. treatment with streptomycin and doxycycline resulted in complete healing. exceptionally, and only if the epidemiologic context supports it, ... | 2002 | 12195883 |
| global analysis of the brucella melitensis proteome: identification of proteins expressed in laboratory-grown culture. | brucella melitensis is a facultative intracellular bacterial pathogen that causes brucellosis, a zoonotic disease primarily infecting sheep and goats, characterized by undulant fever, arthritic pain and other neurological disorders in humans. a comprehensive proteomic study of strain 16m was conducted to identify and characterize the proteins expressed in laboratory-grown culture. using overlapping narrow range immobilized ph gradient strips for two-dimensional gel electrophoresis, 883 protein s ... | 2002 | 12203900 |
| brucella spondylitis with paravertebral abscess due to brucella melitensis infection: a case report. | this report describes the case of a 45-year-old woman with a 5-month history of fever, generalized malaise, myalgia, lower back pain and difficulty in walking. serodiagnosis for brucella, carried out at the onset of symptoms 5 months previously, was negative. when the patient was admitted to our hospital there was contracture of the paraspinal muscles but no peripheral nerve damage. laboratory tests showed positive agglutination for brucella and an increase in the rate of dilution from 1/160 to ... | 2002 | 12224383 |
| effect of omp10 or omp19 deletion on brucella abortus outer membrane properties and virulence in mice. | the distinctive properties of brucella outer membrane have been considered to be critical for brucella sp. virulence. among the outer membrane molecules possibly related to these properties, omp10 and omp19 are immunoreactive outer membrane lipoproteins. moreover, these proteins of brucella could constitute a new family of outer membrane proteins specifically encountered in the family rhizobiaceae: we evaluated the impact of omp10 or omp19 deletion on brucella abortus outer membrane properties a ... | 2002 | 12228280 |
| an overview of the eradication of brucella melitensis from kwazulu-natal. | brucella melitensis is a gram-negative bacterium whose primary hosts are goats and sheep. like the other brucelia spp., with the exception of brucella ovis, it is not particularly host specific as it is pathogenic for a variety of other mammal species including humans. in humans the disease caused by it is rated as one of the most important zoonoses. three outbreaks have been recorded in goats and sheep in south africa; the first outbreak occurred in sheep in 1965 in the mpumalanga and northern ... | 2002 | 12233997 |
| the brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. | the 3.31-mb genome sequence of the intracellular pathogen and potential bioterrorism agent, brucella suis, was determined. comparison of b. suis with brucella melitensis has defined a finite set of differences that could be responsible for the differences in virulence and host preference between these organisms, and indicates that phage have played a significant role in their divergence. analysis of the b. suis genome reveals transport and metabolic capabilities akin to soil/plant-associated bac ... | 2002 | 12271122 |
| comparative genome analysis of the alpha -proteobacteria: relationships between plant and animal pathogens and host specificity. | 2002 | 12271145 | |
| a review of camel brucellosis. | we reviewed the literature on camel brucellosis. the seroprevalence of brucellosis in camels appears to follow two distinct patterns: a low (2-5%) prevalence in nomadic or extensively kept camels and a high (8-15%) prevalence in camels kept intensively or semi-intensively. the infection is caused by different biotypes of brucella abortus and brucella melitensis. many gaps exist in the literature on the epidemiology of camel brucellosis. there is no clear policy in any of the camel-keeping countr ... | 2002 | 12324206 |
| use of the isolator 1.5 microbial tube for detection of brucella melitensis in synovial fluid. | 2002 | 12354908 | |
| comparison of three different pcr methods for detection of brucella spp in human blood samples. | in the diagnosis of human brucellosis, pcr could be a more sensitive technique than blood cultures and more specific than conventional serological tests. we compared three different pcr methods for the detection of brucella spp. and we studied whether human genomic dna affect the sensitivity of three primer pairs for the detection of brucella dna in a peripheral-blood pcr assay. these three pairs of primers amplified three different fragments included in: (i). a gene encoding a 31-kda brucella a ... | 2002 | 12381466 |
| global analysis of brucella melitensis proteomes. | brucella melitensis is a facultative, intracellular, gram-negative cocco-bacillus that causes malta fever in humans and brucellosis in animals. there are at least six species in the genus, and the disease is classified as zoonotic because several species infect humans. using 2-d gel electrophoresis and mass spectrometry, we have initiated (i) a comprehensive mapping and identification of all the expressed proteins of b. melitensis virulent strain 16m, and (ii) a comparative study of its proteome ... | 2002 | 12381571 |
| combined s99/rb51 antigen for complement fixation test for serological diagnosis of brucellosis in cattle and sheep. | to assess the efficiency of a single antigen for the complement fixation (cf) test, prepared by combining brucella abortus smooth strain 99 (s99) with brucella abortus rough strain rb51(rb51), in detecting cattle and sheep infected or vaccinated with brucella spp. | 2002 | 12392535 |
| evolutionary implications of phylogenetic analyses of the gene transfer agent (gta) of rhodobacter capsulatus. | the gene transfer agent (gta) of the a-proteobacterium rhodobacter capsulatus is a cell-controlled genetic exchange vector. genes that encode the gta structure are clustered in a 15-kb region of the r. capsulatus chromosome, and some of these genes show sequence similarity to known bacteriophage head and tail genes. however, the production of gta is controlled at the level of transcription by a cellular two-component signal transduction system. this paper describes homologues of both the gta str ... | 2002 | 12399927 |
| brucellosis in central america. | brucellosis is a disease of domestic animals and humans in central america (ca). bovine and swine brucellosis caused by brucella abortus and brucella suis, respectively, have been identified in all ca countries, while ovine and caprine brucellosis caused by brucella melitensis has been detected in guatemala. the prevalence of bovine brucellosis is estimated between 4 and 8%, with higher prevalence in dairy herds, with losses calculated at 25 million us dollars per year. national control programs ... | 2002 | 12414131 |
| incidence and control of brucellosis in the near east region. | in countries of the near east region, brucellosis was reported in almost all domestic animals, particularly cattle, sheep and goats. brucellosis in camels has been reported in saudi arabia, kuwait, oman, iraq, iran, sudan, egypt, libya and somalia. it has been reported even in racing camels in the united arab emirates. in egypt, brucellosis has been reported also in buffaloes, equines and swine. brucella melitensis biovar 3 is the most commonly isolated species from animals in egypt, jordan, isr ... | 2002 | 12414137 |
| brucellosis in the european union and norway at the turn of the twenty-first century. | control and eradication programs of brucellosis in cattle, sheep, goats and pigs have been more or less successfully implemented within the member states (ms) of the european union (eu) and norway after word war ii. as a result, the epidemiological situation of animal brucellosis is extremely diverse among different ms or regions within a ms and among the different animal species. some ms, mainly north european countries, and norway are declared "officially bovine brucellosis free" and/or "offic ... | 2002 | 12414139 |
| an overview of the epidemiology and epizootology of brucellosis in selected countries of central and southeast europe. | the objective of this paper is to give an overview of the epidemiologic and epizootic status of brucellosis in selected countries of central and southeast europe (balkan region). based on dimension of the disease problem, there is a need to establish collaboration in the eradication and prevention of brucellosis between all countries in the region. although there were no readily accessible data concerning epidemiology and epizootology of brucellosis in these countries, the limited official and p ... | 2002 | 12414140 |
| an epidemiological and surveillance overview of brucellosis in romania. | this article reports epidemiological investigations on the occurrence of brucellosis in romania. like in other former communist countries, data concerning epidemiology of brucellosis and published articles are very few. the epidemiology and control of brucellosis in romania was analyzed using data made available by the office international des epizooties and veterinary service of romania. romania, like many other developed countries, has eradicated brucella abortus from cattle since 1969. brucel ... | 2002 | 12414141 |
| epidemiology and control of brucellosis in china. | the paper describes the history and evolvement of brucellosis in china. it presents the variation of epidemic situation, epidemiological characteristics, application of vaccines and control in brief. before 1980s, human and animal brucellosis was quite severe; during 1980s, the incidence of human and animal brucellosis was relatively low, and seemed to decrease during the decade. during 1990s, there were no obvious changes in the incidence of animal brucellosis, but the incidence of human brucel ... | 2002 | 12414142 |
| epidemiology, zoonotic aspects, vaccination and control/eradication of brucellosis in india. | in india, brucellosis was first recognised in 1942 and is now endemic throughout the country. the disease is reported in cattle, buffalo, sheep, goats, pigs, dogs and humans. b. abortus biotype-1 in cattle and buffaloes and b. melitensis biotype-1 in sheep, goats and man are the predominant infective biotypes. the long-term serological studies have indicated that 5% of cattle and 3% of buffaloes are infected with brucellosis. economic losses due to brucellosis in livestock are considerable in an ... | 2002 | 12414143 |
| brucella evolution and taxonomy. | the genus brucella contains alpha-proteobacteria adapted to intracellular life within cells of a variety of mammals. controversy has arisen concerning brucella internal taxonomy, and it has been proposed that the dna-dna hybridization-based genomospecies concept be applied to the genus. according to this view, only one species, brucella melitensis, should be recognized, and the classical species should be considered as biovars (b. melitensis biovar melitensis; b. melitensis biovar abortus; etc.) ... | 2002 | 12414145 |
| major outer membrane proteins of brucella spp.: past, present and future. | the major outer membrane proteins (omps) of brucella spp. were initially identified in the early 1980s and characterised as potential immunogenic and protective antigens. they were classified according to their apparent molecular mass as 36-38 kda omps or group 2 porin proteins and 31-34 and 25-27 kda omps which belong to the group 3 proteins. the genes encoding the group 2 porin proteins were identified in the late 1980s and consist of two genes, omp2a and omp2b, which are closely linked in the ... | 2002 | 12414146 |
| the myth of brucella l-forms and possible involvement of brucella penicillin binding proteins (pbps) in pathogenicity. | brucella spp. l-forms have been proposed to be stationary phase organisms in the evolution of new variants and enduring entities in the host in complicated cases of brucellosis and during latent brucellosis. in vitro formation of brucella l-forms has been achieved by treating the cells with sub-lethal doses of penicillin. interestingly, brucella spp. have classified during the evolution into two groups, penicillin susceptible or penicillin resistant, yet both types grow on 20 microg/ml of methic ... | 2002 | 12414148 |
| characterization of the caprine model for ruminant brucellosis. | the relationship between man, the goat, and brucellosis is historical. today brucella melitensis and brucella abortus pose a serious economic and public health threat in many countries throughout the world. infection of pregnant goats and sheep with b. melitensis results in abortion during the third trimester of pregnancy. although nearly eradicated in the us, bovine brucellosis is still a problem in many countries and the potential for re-infection of domestic stock from wildlife reservoirs in ... | 2002 | 12414161 |
| brucellosis vaccines: past, present and future. | the first effective brucella vaccine was based on live brucella abortus strain 19, a laboratory-derived strain attenuated by an unknown process during subculture. this induces reasonable protection against b. abortus, but at the expense of persistent serological responses. a similar problem occurs with the b. melitensis rev.1 strain that is still the most effective vaccine against caprine and ovine brucellosis. vaccines based on killed cells of virulent strains administered with adjuvant induced ... | 2002 | 12414166 |
| control of small ruminant brucellosis by use of brucella melitensis rev.1 vaccine: laboratory aspects and field observations. | brucellosis vaccines are essential elements in control programs. since first developed in the mid-1950s, the brucella melitensis vaccine strain rev.1 has been used worldwide and its significant value in protecting sheep and goats in endemic areas recognized. this review provides historical background on the development of the vaccine, its use and field complications arising in israel following changes in the strain's pathogenicity. the urgent need for resolving cases of vaccine strain excretion ... | 2002 | 12414167 |
| the genome of brucella melitensis. | the genome of brucella melitensis strain 16m was sequenced and contained 3,294,931 bp distributed over two circular chromosomes. chromosome i was composed of 2,117,144 bp and chromosome ii has 1,177,787 bp. a total of 3,198 orfs were predicted. the origins of replication of the chromosomes are similar to each other and to those of other alpha-proteobacteria. housekeeping genes such as those that encode for dna replication, protein synthesis, core metabolism, and cell-wall biosynthesis were found ... | 2002 | 12414174 |
| brucella proteomes--a review. | the proteomes of selected brucella spp. have been extensively analyzed by utilizing current proteomic technology involving 2-de and maldi-ms. in brucella melitensis, more than 500 proteins were identified. the rapid and large-scale identification of proteins in this organism was accomplished by using the annotated b. melitensis genome which is now available in the genbank. coupled with new and powerful tools for data analysis, differentially expressed proteins were identified and categorized int ... | 2002 | 12414175 |
| risk-based design of repeated surveys for the documentation of freedom from non-highly contagious diseases. | the documentation of freedom from disease requires reliable information on the actual disease status in a specific animal population. the implementation of active surveillance (surveys) is an effective method to gain this information. for economical reasons, the sample size should be as small as possible but large enough to achieve the required confidence level for a targeted threshold. when conducting surveys repeatedly, various information sources about the disease status of the population can ... | 2002 | 12441234 |
| detection of brucella melitensis by the bact/alert automated system and brucella broth culture. | this study was conducted to evaluate the ability of the bact/alert automated blood culture system to detect brucella spp. in comparison with traditional brucella broth culture. overall, 100 (50 bone marrow and 50 blood samples) paired cultures were obtained, and 59 were positive by at least one method. the brucella broth culture method detected all 59 positive cultures (100%), and the bact/alert system detected 30 (50.8%) (p < 0.05). the mean detection times for b. melitensis were 4.5 days in th ... | 2002 | 12445015 |
| increased pleural fluid adenosine deaminase in brucellosis is difficult to differentiate from tuberculosis. | pleural involvement in brucellosis is very rare. current knowledge on brucella pleuritis is limited to a few case studies, and pleural adenosine deaminase (ada) in brucellosis has not been studied previously. we report the pleural fluid characteristics, including ada, of two cases with brucella pleurisy. analysis of the pleural fluids revealed exudative effusions with increased ada level, decreased glucose concentration, and lymphocyte predominance. the similarity with tuberculous pleurisy was r ... | 2002 | 12457012 |
| evaluation of a new immunocapture test for the diagnosis of ovine brucellosis caused by brucella melitensis. | a new immunocapture technique has been applied to the diagnosis of ovine brucellosis under experimental conditions. the tests were made on a serum bank derived from both young and adult ewes vaccinated conjunctivally with the rev 1 strain at a dose of 10(8) to 10(9) colony-forming units. adult ewes were infected experimentally two-and-a-half years after they had been vaccinated and the results were compared with an unvaccinated control group. the condition of each animal in terms of infection wi ... | 2002 | 12479299 |
| molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans. | brucellosis caused by brucella spp. is a major zoonotic disease. control of brucellosis in agricultural animals is a prerequisite for the prevention of this disease in human beings. recently, brucella melitensis was declared by the centers for disease control and prevention to be one of three major bioterrorist agents due to the expense required for the treatment of human brucellosis patients. also, the economic agricultural loss due to bovine brucellosis emphasizes the financial impact of bruce ... | 2003 | 12525425 |
| late reactivation of calcified granuloma in a patient with chronic suppurative brucellosis. | 2002 | 12525929 | |
| prediction and overview of the rpon-regulon in closely related species of the rhizobiales. | in the rhizobia, a group of symbiotic gram-negative soil bacteria, rpon (sigma54, sigman, ntra) is best known as the sigma factor enabling transcription of the nitrogen fixation genes. recent reports, however, demonstrate the involvement of rpon in other symbiotic functions, although no large-scale effort has yet been undertaken to unravel the rpon-regulon in rhizobia. we screened two complete rhizobial genomes (mesorhizobium loti, sinorhizobium meliloti) and four symbiotic regions (rhizobium et ... | 2002 | 12537565 |
| brucella melitensis endocarditis of ventricular septal defect patch: successful surgical treatment without replacement. | 2003 | 12539006 | |
| nramp1 is not a major determinant in the control of brucella melitensis infection in mice. | brucella, the causative agent of brucellosis in animals and humans, can survive and proliferate within macrophages. macrophages mediate mouse resistance to various pathogens through the expression of the nramp1 gene. the role of this gene in the control of brucella infection was investigated. when balb/c mice (nramp1(s)) and c.cb congenic mice (nramp1(r)) were infected with brucella melitensis, the number of brucella organisms per spleen was significantly larger in the c.cb mice than in the balb ... | 2003 | 12540538 |
| biosynthesis of phosphatidylcholine in bacteria. | phosphatidylcholine (pc) is the major membrane-forming phospholipid in eukaryotes and can be synthesized by either of two pathways, the methylation pathway or the cdp-choline pathway. many prokaryotes lack pc, but it can be found in significant amounts in membranes of rather diverse bacteria and based on genomic data, we estimate that more than 10% of all bacteria possess pc. enzymatic methylation of phosphatidylethanolamine via the methylation pathway was thought to be the only biosynthetic pat ... | 2003 | 12547654 |
| optimum detection times for bacteria and yeast species with the bactec 9120 aerobic blood culture system: evaluation for a 5-year period in a turkish university hospital. | we tracked and documented the time of positivity of blood cultures by using the bactec 9120 (becton dickinson diagnostic instrument systems) blood culture system over a 5-year study period. a 7-day protocol of the incubation period was selected, and a total of 11156 blood cultures were evaluated. the clinically significant microorganisms (32.95%) were isolated in 3676 specimens. gram-positive and -negative bacterial isolation rates were found to be 41.07 and 44.88%, respectively. yeasts were fou ... | 2003 | 12574291 |
| serological diagnosis of brucellosis caused by brucella canis. | blood serum samples from 2,328 dogs were tested to detect antibodies against brucella canis with the agar gel immunodiffusion (agid) and 2-mercaptoethanol slide agglutination test (me-sat) using brucella ovis as the antigen. all blood serum samples were also evaluated for antibodies against brucella abortus and brucella melitensis using the rose bengal test. twentyfive (1.07%) of the sera evaluated were considered positive with agid test. only 4 (16%) of these blood serum samples were positive w ... | 2003 | 12578313 |
| epidemiological, clinical and laboratory features of brucellosis: a prospective evaluation of 120 adult patients. | this prospective study was carried out in the department of infectious diseases of gaziantep university between january 1997 and december 1999 to evaluate the epidemiological, clinical and laboratory features of brucellosis in south-eastern turkey. one hundred and twenty consecutive patients with active brucellosis were enrolled. the commonest way of transmission was ingestion of milk products from diseased animals. brucella melitensis was isolated in the specimens of 31 (45.5%) of 68 patients. ... | 2003 | 12587937 |
| comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. | pcr assay has been shown to be a promising option for the diagnosis of brucellosis. however, few studies have been performed with field samples in order to evaluate the assay as a diagnostic tool. in this study, routine use of a species-specific pcr assay previously developed for the identification of brucella cultures was assessed for the detection of brucella dna directly from the stomach contents of aborted sheep fetuses. the assay is based on the insertion sequence is711 in the brucella chro ... | 2003 | 12591206 |
| production of the type iv secretion system differs among brucella species as revealed with virb5- and virb8-specific antisera. | expression of the virb operon, encoding the type iv secretion system required for brucella suis virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic ph values. to analyze the production of virb proteins, polyclonal antisera against b. suis virb5 and virb8 were generated. western blot analysis revealed that virb5 and virb8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation. unl ... | 2003 | 12595417 |
| antibodies to the cp24 protein of brucella melitensis lack diagnostic usefulness in ovine brucellosis. | the potential diagnostic usefulness of antibodies to the ribosome recycling factor of brucella melitensis (cp24) was assessed in sheep by an indirect elisa with purified recombinant cp24. sera from uninfected animals from the uk (n=44) and from local flocks (n=42), from sheep naturally infected with b. melitensis (n=12) or b. ovis (n=12), and from lambs (n=7) or pregnant ewes (n=6) vaccinated with b. melitensis rev-1, were assayed. high specific optical densities (od(with antigen) - od(without a ... | 2003 | 12636998 |
| the recombinant omp31 from brucella melitensis alone or associated with rough lipopolysaccharide induces protection against brucella ovis infection in balb/c mice. | immunogenicity and protective activity against brucella ovis of detergent-extracted recombinant omp31 (romp31 extract) from brucella melitensis produced in escherichia coli, purified rough lipopolysaccharide from b. ovis (r-lps) and a mixture of romp31 extract and r-lps (romp31 extract + r-lps) were assessed in balb/c mice. the experimental vaccines were compared with a hot saline extract (hs extract) from b. ovis mainly composed of outer membrane proteins (omps) and r-lps, and known to be prote ... | 2003 | 12650766 |
| brucella prosthetic joint infection: a report of 3 cases and a review of the literature. | we report 3 cases of brucella melitensis infection of prosthetic hips and knees, and we summarize data about 4 cases reported in the literature. six of the 7 affected patients were men. the median duration from prosthesis implantation to the onset of symptoms was 38.7 months. five patients had only local symptoms. preoperative joint aspirates yielded negative culture results for 3 patients, and blood culture results were negative for 6 patients. excisional arthroplasty was the initial interventi ... | 2003 | 12652405 |
| [evaluation of the methods used for the serologic diagnosis of brucellosis]. | serological tests are the most widely used methods for the diagnosis of brucella infections, since bacterial isolation techniques are time consuming. the conventional serological methods are the tube and slide agglutination tests, however enzyme immunoassays (elisa) have gained importance recently as rapid diagnostic techniques. the aim of this study was to evaluate the performances of standard tube agglutination (sta, wright test), slide agglutination (rose-bengal, rb) and elisa-igg and -igm me ... | 2002 | 12652868 |
| therapeutic effect of spiramycin in brucellosis. | this study was undertaken to investigate the usefulness of spiramycin in treatment for brucellosis in an animal model. | 2003 | 12654065 |
| interactions between brucella melitensis and human phagocytes: bacterial surface o-polysaccharide inhibits phagocytosis, bacterial killing, and subsequent host cell apoptosis. | brucellae are gram-negative intracellular pathogens that survive and multiply within host phagocytic cells. smooth organisms present o-polysaccharides (ops) on their surface. the wboa gene, which codes for the enzyme glycosyl transferase, is essential for the assembly of o-chain in brucella. deletion of wboa in smooth, virulent b. melitensis 16m results in a rough mutant designated wrr51. unlike b. abortus, both smooth and rough strains of b. melitensis are resistant to complement-mediated killi ... | 2003 | 12654833 |
| brucella melitensis infection stimulates an immune response leading to kikuchi-fujimoto disease. | a 43-year-old greek cattler with a history of brucellosis three months previously for which he was treated with sulfonamides for three weeks, was admitted to the hospital complaining of fever, arthralgias, night sweats, painful cervical and axillary lymph nodes as well as a weight loss of 8 kg in the previous four months. since microbiological and serological studies did not give a specific diagnosis, an open cervical lymph node biopsy was performed. the histological examination revealed kikuchi ... | 2003 | 12655790 |
| [maculopapular eruption with fluctuating fever]. | 2003 | 12671590 | |
| [ciprofloxacin in the treatment of patients with brucellosis]. | with the aim to estimate the clinical and immunological efficiency of the ciprofloxacin (cifloxinal) 105 patients with acute (51), subacute (19) and chronic (35) brucellosis were studied. control group (17 patients with acute and 30 patients with chronic brucellosis) have been treated with combination of two antibiotics: doxycycline and rifampicin. ciprofloxacin in a dose 500 mg bid within 14 days in acute stage and 20 days in chronic stage of disease essentially reduced duration of local inflam ... | 2002 | 12674788 |
| brucella melitensis bacteremia in children: review of 62 cases. | brucella bacteremia is not uncommon in children living in endemic areas. reports on brucella bacteremia, however, are scarce. its clinical features and complications are unknown. this retrospective review describes the clinical and laboratory characteristics, the relapse rate, and response to different regimens of antimicrobials in children with brucella bacteremia over a 5-year period. antimicrobial susceptibility testing was performed on all isolates. data on 62 children with brucella bacterem ... | 2003 | 12678419 |
| [anti-lysozyme activity of bacteria of the genus brucella]. | the antilysozyme activity of 122 brucella strains has been studied. the antilysozyme sign has been found to be widely spread among different brucella species. the antilysozyme sign has proved to be most pronounced in b. melitensis. | 2000 | 12712527 |
| endemic brucellar epididymo-orchitis: a 10-year experience. | to present epidemiologic, clinical and laboratory features, treatment and outcome of patients suffering from brucella melitensis-induced epididymo-orchitis, in comparison with cases of nonspecific epididymo-orchitis. distinction between these two entities is essential, as treatment and outcome are entirely different. | 2002 | 12718826 |
| brucellosis in children: clinical observations in 115 cases. | brucellosis is endemic in saudi arabia. this report summarizes the epidemiology of brucellosis in children. | 2002 | 12718832 |
| [subphrenic abscess during the course of acute brucellosis: diagnosis and treatment with percutaneous sonographically-guided drainage]. | abscess formation during the course of acute brucellosis is a rare event. a case of subphrenic abscess, the first to our knowledge, is described. a 49 years-old male patient with fever and a mild increase in alt and gamma-gt was referred to our institution. routine blood exams tested negative and antibodies against brucella spp. were also negative. ct examination of abdomen was normal. after 8 days, us examination showed a liquid area under the right diaphragma and us-guided puncture revealed an ... | 2003 | 12719669 |
| misidentification of brucella melitensis as ochrobactrum anthropi by api 20ne. | 2003 | 12721322 | |
| [brucellosis]. | 2003 | 12722260 | |
| type iii secretion homologs are present in brucella melitensis, b. ovis, and b. suis biovars 1, 2, and 3. | protein sequences from characterized type iii secretion (tts) systems were used as probes in silico to identify several tts gene homologs in the genome sequence of brucella suis biovar 1 strain 1330. four of the genes, named flhb, flip, flir, and flif on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in pcr and hybridization assays to determine their distribution among other brucella nomen species and biovars. the results indicated that flhb, flip, flir and ... | 2003 | 12732970 |
| isolation and expression of recombinant antibody fragments to the biological warfare pathogen brucella melitensis. | brucella melitensis is a highly infectious animal pathogen able to cause a recurring debilitating disease in humans and is therefore high on the list of biological warfare agents. immunoglobulin genes from mice immunized with gamma-irradiated b. melitensis strain 16m were used to construct a library that was screened by phage display against similarly prepared bacteria. the selected phage particles afforded a strong enzyme-linked immunosorbent assay (elisa) signal against gamma-irradiated b. mel ... | 2003 | 12738372 |
| spontaneous bacterial peritonitis due to brucella melitensis. | peritonitis is an extremely rare complication of brucellosis. a case is reported of blood and ascitic culture-proven spontaneous bacterial peritonitis caused by brucella melitensis, in a patient who had also cirrhosis. | 2003 | 12751716 |
| acute abdomen due to brucella melitensis. | a case of acute abdomen caused by a brucella melitensis is reported. the patient presented with biliary involvement in the form of acute acalculous cholecystitis and developed acute appendicitis that resulted in his surgical treatment. | 2003 | 12751720 |
| [sarcoidosis and brucellosis: a strange and infrequent association]. | a cattlewoman, a 63-year-old patient with sarcoidosis associated to mellitensis brucellosis, was studied. clinical manifestations of both multisystemic diseases were compared, as well as diagnostic and therapeutic aspects; we concluded that coexistence of both entities is infrequent but may occur due to increased presentation of the diseases. | 2003 | 12754952 |
| predicting gene expression levels from codon biases in alpha-proteobacterial genomes. | predicted highly expressed (phx) genes in five currently available high g+c complete alpha-proteobacterial genomes are analyzed. these include: the nitrogen-fixing plant symbionts sinorhizobium meliloti (sinme) and mesorhizobium loti (meslo), the nonpathogenic aquatic bacterium caulobacter crescentus (caucr), the plant pathogen agrobacterium tumefaciens (agrtu), and the mammalian pathogen brucella melitensis (brume). three of these genomes, sinme, agrtu, and brume, contain multiple chromosomes o ... | 2003 | 12775761 |
| brucellar spinal epidural abscess. | 2003 | 12776958 | |
| effect of bacterial extracts on the immunologic profile in chronic relapsing brucellosis patients. | brucellosis is an intracellular bacterial disease of common incidence in greece. existing therapy is inadequate and a considerable proportion of patients become chronically ill and are immunocompromised. defects of the monocyte-macrophage system and t-lymphocytes have been described in chronic brucellosis and can be restored after immunopotentiation therapy. bacterial (klebsiella pneumoniae) extracts exert immunostimulating effects on the monocyte-macrophage system and have already been used suc ... | 1999 | 12783653 |
| evaluation of a modified rose bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of brucella melitensis infection in sheep. | a modified rose bengal test (mrb) and an indirect elisa (ielisa) with protein g as the conjugate, were evaluated for the diagnosis of brucella melitensis infection in unvaccinated sheep with a known bacteriological status, and their diagnostic efficacy was compared with that of the standard rose bengal (rb) and complement fixation (cf) tests used in the current eradication campaign in eu countries. all tests showed 100% specificity when testing the sera from 212 brucella-free sheep. when testing ... | 2003 | 12791239 |