Publications

genetic diversity, reassortment, and recombination in alfalfa mosaic virus population in spain.	the variability and genetic structure of alfalfa mosaic virus (amv) in spain was evaluated through the molecular characterization of 60 isolates collected from different hosts and different geographic areas. analysis of nucleotide sequences in four coding regions--p1, p2, movement protein (mp), and coat protein (cp)--revealed a low genetic diversity and different restrictions to variation operating on each coding region. phylogenetic analysis of spanish isolates along with previously reported am ...	2014	24779352
comparative mapping of the wild perennial glycine latifolia and soybean (g. max) reveals extensive chromosome rearrangements in the genus glycine.	soybean (glycine max l. mer.), like many cultivated crops, has a relatively narrow genetic base and lacks diversity for some economically important traits. glycine latifolia (benth.) newell & hymowitz, one of the 26 perennial wild glycine species related to soybean in the subgenus glycine willd., shows high levels of resistance to multiple soybean pathogens and pests including alfalfa mosaic virus, heterodera glycines ichinohe and sclerotinia sclerotiorum (lib.) de bary. however, limited informa ...	2014	24937645
the photosystem ii oxygen-evolving complex protein psbp interacts with the coat protein of alfalfa mosaic virus and inhibits virus replication.	alfalfa mosaic virus (amv) coat protein (cp) is essential for many steps in virus replication from early infection to encapsidation. however, the identity and functional relevance of cellular factors that interact with cp remain unknown. in an unbiased yeast two-hybrid screen for cp-interacting arabidopsis proteins, we identified several novel protein interactions that could potentially modulate amv replication. in this report, we focus on one of the novel cp-binding partners, the arabidopsis ps ...	2014	24940990
viral and chloroplastic signals essential for initiation and efficiency of translation in agrobacterium tumefaciens.	the construction of high-level protein expression vectors using the camv 35s promoter in concert with highly efficient translation initiation signals for agrobacterium tumefaciens is a relatively less explored field compared to that of escherichia coli. in the current study, we experimentally investigated the capacity of the camv 35s promoter to direct gfp gene expression in a. tumefaciens in the context of different viral and chloroplastic translation initiation signals. gfp expression and conc ...	2014	25117444
resistance breaking tomato spotted wilt virus isolates on resistant pepper varieties in italy.	in spring 2012, resistance breaking (rb) isolates of tomato spotted wilt virus (tswv) that overcome the resistance conferred by the tsw gene in different pepper hybrids have been recovered in different locations in southern italy (campania and apulia regions) in protected cultivation, about one month after transplant. the percentage of symptomatic plants was 5-10% and, only in particular cases of advanced stage of cultivation, it reached 30-50% at the end of cycle. all tswv isolates induced simi ...	2013	25151838
biological and molecular variability of alfalfa mosaic virus affecting alfalfa crop in riyadh region.	in 2011-2012, sixty nine samples were collected from alfalfa plants showing viral infection symptoms in riyadh region. mechanical inoculation with sap prepared from two collected samples out of twenty five possitive for alfalfa mosaic virus (amv) by elisa were produced systemic mosaic on vigna unguiculata and nicotiana tabacum, local lesion on chenopodium amaranticolor and c. quinoa. vicia faba indicator plants that induce mosaic and mottle with amv-sagir isolate and no infection with amv-wadi a ...	2013	25288969
control of virus diseases in soybeans.	soybean, one of the world's most important sources of animal feed and vegetable oil, can be infected by numerous viruses. however, only a small number of the viruses that can potentially infect soybean are considered as major economic problems to soybean production. therefore, we consider management options available to control diseases caused by eight viruses that cause, or have the potential to cause, significant economic loss to producers. we summarize management tactics in use and suggest di ...	2014	25410106
engineering and expression of a rhoa peptide against respiratory syncytial virus infection in plants.	main conclusion : a rhoa-derived peptide fused to carrier molecules from plants showed enhanced biological activity of in vitro assays against respiratory syncytial virus compared to the rhoa peptide alone or the synthetic rhoa peptide. a rhoa-derived peptide has been reported for over a decade as a potential inhibitor of respiratory syncytial virus (rsv) infection both in vitro and in vivo and is anticipated to be a promising alternative to monoclonal antibody-based therapy against rsv infectio ...	2016	26474991
structural studies on tobacco streak virus coat protein: insights into the pleomorphic nature of ilarviruses.	tobacco streak virus (tsv), the type member of ilarvirus genus, is a major plant pathogen. tsv purified from infected plants consists of a ss-rna genome encapsidated in spheroidal particles with diameters of 27, 30 and 33nm constructed from multiple copies of a single species of coat protein (cp) subunits. apart from protecting the viral genome, cps of ilarviruses play several key roles in the life cycle of these viruses. unlike the related bromo and cucumoviruses, ilarvirus particles are labile ...	2016	26706030
the coat protein of alfalfa mosaic virus interacts and interferes with the transcriptional activity of the bhlh transcription factor ilr3 promoting salicylic acid-dependent defence signalling response.	during virus infection, specific viral component-host factor interaction elicits the transcriptional reprogramming of diverse cellular pathways. alfalfa mosaic virus (amv) can establish a compatible interaction in tobacco and arabidopsis hosts. we show that the coat protein (cp) of amv interacts directly with transcription factor (tf) ilr3 of both species. ilr3 is a basic helix-loop-helix (bhlh) family member of tfs, previously proposed to participate in diverse metabolic pathways. ilr3 has been ...	2017	26929142
the functional analysis of distinct tospovirus movement proteins (nsm) reveals different capabilities in tubule formation, cell-to-cell and systemic virus movement among the tospovirus species.	the lack of infectious tospovirus clones to address reverse genetic experiments has compromised the functional analysis of viral proteins. in the present study we have performed a functional analysis of the movement proteins (nsm) of four tospovirus species bean necrotic mosaic virus (benmv), chrysanthemum stem necrosis virus (csnv), tomato chlorotic spot virus (tcsv) and tomato spotted wilt virus (tswv), which differ biologically and molecularly, by using the alfalfa mosaic virus (amv) model sy ...	2017	27697453
pathogen phytosensing: plants to report plant pathogens.	real-time systems that provide evidence of pathogen contamination in crops can be an important new line of early defense in agricultural centers. plants possess defense mechanisms to protect against pathogen attack. inducible plant defense is controlled by signal transduction pathways, inducible promoters and cis-regulatory elements corresponding to key genes involved in defense, and pathogen-specific responses. identified inducible promoters and cis-acting elements could be utilized in plant se ...	2008	27879840
seed tolerance to deterioration in arabidopsis is affected by virus infection.	seed longevity is the period during which the plant seed is able to germinate. this property is strongly influenced by environment conditions experienced by seeds during their formation and storage. in the present study we have analyzed how the biotic stress derived from the infection of cauliflower mosaic virus (camv), turnip mosaic virus (tumv), cucumber mosaic virus (cmv) and alfalfa mosaic virus (amv) affects seed tolerance to deterioration measuring germination rates after an accelerated ag ...	2017	28477474
within-host evolution of segments ratio for the tripartite genome of alfalfa mosaic virus.	the existence of multipartite viruses is an intriguing mystery in evolutionary virology. several hypotheses suggest benefits that should outweigh the costs of a reduced transmission efficiency and of segregation of coadapted genes associated with encapsidating each segment into a different particle. advantages range from increasing genome size despite high mutation rates, faster replication, more efficient selection resulting from reassortment during mixed infections, better regulation of gene e ...	2017	28694514
detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in saudi arabia.	a total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in saudi arabia. das-elisa test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in saudi arabia. eighty percent of the alfalfa sampl ...	2017	28855829