Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a comparative analysis of the avirulence and translational transactivator functions of gene vi of cauliflower mosaic virus. | the primary function associated at present with the gene vi product of cauliflower mosaic virus (camv) is that of a translational transactivator (tav). in this capacity, it alters the host translational machinery to allow reinitiation of translation of other camv genes on the polycistronic 35s rna of camv. in addition, the gene vi protein can elicit a specific type of plant defense response called the hypersensitive response (hr) in nicotiana edwardsonii. in this study, we have adapted the agroi ... | 2002 | 11886242 |
| delayed abscission and shorter internodes correlate with a reduction in the ethylene receptor leetr1 transcript in transgenic tomato. | stable transformation of tomato (lycopersicon esculentum cv ailsa craig) plants with a construct containing the antisense sequence for the receiver domain and 3'-untranslated portion of the tomato ethylene receptor (leetr1) under the control of an enhanced cauliflower mosaic virus 35s promoter resulted in some expected and unexpected phenotypes. in addition to reduced leetr1 transcript levels, the two most consistently observed phenotypes in the transgenic lines were delayed abscission and reduc ... | 2002 | 11891253 |
| observations concerning the discontinuous dnas of cauliflower mosaic virus. | the double-stranded circular dna encapsidated within cauliflower mosaic virus (camv) particles contains three single-stranded discontinuities, two in one strand and one in the other, so that, upon denaturation, three linear single-stranded dnas are produced. here we show that a fourth much smaller single-stranded dna, termed alpha1, is also present in denatured camv dna preparations. the 5' extremity of alpha1 is identical to that of the alpha-strand, the strand of dna possessing only one interr ... | 1983 | 11894938 |
| growth and differentiation of transgenic callus regulated by phytohormones and antibiotics in transformation of loblolly pine. | mature zygotic embryos of loblolly pine (pinus taeda l.) were transformed by agrobacterium tumefaciens strain lba 4404 harbouring the plasmid pbi121 which carried the selectable marker gene, neomycin phosphotransferase ii (npt ii) controlled by the promoter of the nopaline synthase gene, and the uida reporter gene, encoding beta-glucuronidase (gus) driven by the cauliflower mosaic virus 35s promoter. organogenic transgenic calli and transgenic regenerated plantlets were produced on selection med ... | 2002 | 11902001 |
| activation of the promoters of arabidopsis genes for the branched-chain alpha-keto acid dehydrogenase complex in transgenic tobacco by-2 cells under sugar starvation. | sugar starvation exerted by sub-10 mm levels of sucrose on arabidopsis t87 suspension-cultured cells triggered marked accumulation of the transcripts of genes for e1beta and e2 subunit of the branched-chain alpha-keto acid dehydrogenase complex. similar levels of sugar starvation increased the luciferase activity in transgenic tobacco by-2 lines expressing the arabidopsis e1beta- or e2-promoter-luciferase fusion gene. these results indicate that sugar levels tightly regulate the e1beta and e2 pr ... | 2002 | 11917081 |
| mycorrhizal colonization of transgenic aspen in a field trial. | mycorrhizal colonization of genetically modified hybrid aspen (populus tremula x p. tremuloides michx.) was investigated over 15 months in a field experiment. the aspen carried the rolc gene from agrobacterium rhizogenes under control of either the constitutive cauliflower mosaic virus 35s promoter or the light-inducible rbcs promoter. arbuscular mycorrhizas (ams) were rare in all root samples, while fully developed ectomycorrhizas (ems) were found in all samples. no significant differences in t ... | 2002 | 11925050 |
| biodiversity (communications arising): maize transgene results in mexico are artefacts. | quist and chapela's conclusion that the transgenes they claim to have detected in native maize in oaxaca, mexico, are predominantly reassorted and inserted into a "diversity of genomic contexts" seems to be based on an artefact arising from the inverse polymerase chain reaction (i-pcr) they used to amplify sequences flanking 35s transgenes from cauliflower mosaic virus (camv). | 2002 | 11935145 |
| camv 35s promoter directs beta-glucuronidase expression in ganoderma lucidum and pleurotus citrinopileatus. | the cauliflower mosaic virus (camv) 35s promoter has been most commonly used in plant transformation studies, but its activity in mushrooms has not been reported. p301-b is a binary vector containing a bialaphos resistance gene driven by the promoter of lentinus edodes glyceraldehyde-3-phosphate dehydrogenase (gpd) gene. camv 35s-gus was inserted into p301-b, and the resulting construct p301-bg was transformed to protoplasts of ganoderma lucidum and basidiospores of pleurotus citrinopileatus. gu ... | 2002 | 11936254 |
| over-expression of cinnamate 4-hydroxylase leads to increased accumulation of acetosyringone in elicited tobacco cell-suspension cultures. | cell-suspension cultures were produced from transgenic tobacco (nicotiana tabacum l.) plants harboring a constitutively expressed alfalfa cinnamate 4-hydroxylase (c4h) transgene. increased levels of c4h enzyme activity in the transgenic cultures were observed only following exposure of the cells to yeast elicitor, although alfalfa c4h transcripts were expressed at a high level from the cauliflower mosaic virus 35s promoter in the absence of elicitation. increased expression of c4h in elicited ce ... | 2002 | 11941467 |
| the sambucus nigra type-2 ribosome-inactivating protein sna-i' exhibits in planta antiviral activity in transgenic tobacco. | transgenic tobacco (samsun nn) plants transformed with a cdna clone encoding sna-i' from sambucus nigra synthesize, and correctly process and assemble, a fully active type-2 ribosome-inactivating protein. expression of sna-i' under the control of the 35s cauliflower mosaic virus promoter enhances the plant's resistance against infection with tobacco mosaic virus. in contrast to type-1 ribosome-inactivating proteins, the expression of sna-i' does not affect the growth and fertility of the transge ... | 2002 | 11959096 |
| overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. | polyphenol oxidases (ppos; ec 1.10.3.2 or ec 1.14.18.1) catalyzing the oxygen-dependent oxidation of phenols to quinones are ubiquitous among angiosperms and assumed to be involved in plant defense against pests and pathogens. in order to investigate the role of ppo in plant disease resistance, we made transgenic tomato ( lycopersicon esculentum mill. cv. money maker) plants that overexpressed a potato ( solanum tuberosum l.) ppo cdna under control of the cauliflower mosaic virus 35s promoter. t ... | 2002 | 12029473 |
| expression of recombinant trichosanthin, a ribosome-inactivating protein, in transgenic tobacco. | trichosanthin (tcs) is an antiviral plant defense protein, classified as a type-i ribosome-inactivating protein, found in the root tuber and leaves of the medicinal plant trichosanthes kirilowii. it is processed from a larger precursor protein, containing a 23 amino acid amino (n)-terminal sequence (pre sequence) and a 19 amino acid carboxy (c)-terminal extension (pro sequence). various constructs of the tcs gene were expressed in transgenic tobacco plants to determine the effects of the amino- ... | 2002 | 12052684 |
| potentiation of developmentally regulated plant defense response by atwrky18, a pathogen-induced arabidopsis transcription factor. | atwrky18 is a pathogen- and salicylic acid-induced arabidopsis transcription factor containing the plant-specific wrky zinc finger dna-binding motif. in the present study, we have transformed arabidopsis plants with atwrky18 under control of the cauliflower mosaic virus 35s promoter. surprisingly, transgenic plants expressing high levels of atwrky18 were stunted in growth. when expressed at moderate levels, atwrky18 potentiated developmentally regulated defense responses in transgenic plants wit ... | 2002 | 12068113 |
| evidence for a ustilago maydis steroid 5alpha-reductase by functional expression in arabidopsis det2-1 mutants. | we have identified a gene (udh1) in the basidiomycete ustilago maydis that is induced during the parasitic interaction with its host plant maize (zea mays). udh1 encodes a protein with high similarity to mammalian and plant 5alpha-steroid reductases. udh1 differs from those of known 5alpha-steroid reductases by six additional domains, partially predicted to be membrane-spanning. a fusion protein of udh1 and the green fluorescent protein provided evidence for endoplasmic reticulum localization in ... | 2002 | 12068114 |
| a complex fruit-specific type-2 ribosome-inactivating protein from elderberry (sambucus nigra) is correctly processed and assembled in transgenic tobacco plants. | fruits of elderberry (sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the neuac(alpha2,6)gal/galnac disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the b-chains of two adjacent protomers. a cdna clone encoding this so-called sambucus nigra fruit specific agglutinin i (sna-if) was isolated and expressed in tobacco (samsun nn) under the control of the 35s cauliflower mosa ... | 2002 | 12071953 |
| regulated nuclear targeting of cauliflower mosaic virus. | the mature cauliflower mosaic virus (camv) capsid protein (cp), if expressed in the absence of other viral proteins, is transported into the plant cell nucleus by the action of a nuclear localization signal (nls) close to the n terminus. in contrast, virus particles do not enter the nucleus, but dock at the nuclear membrane, a process inhibited by anti-nls antibodies or by gtp gamma s, and apparently mediated by interaction of cp with host importin alpha. the very acidic n-terminal extension of ... | 2002 | 12075100 |
| isolate-specific synergy in disease symptoms between cauliflower mosaic and turnip vein-clearing viruses. | simultaneous infection of a plant by two viruses can cause more severe disease than is caused by infection with either virus alone. such synergy may be due to effects on the replication of one virus by the second virus or to other causes. the tobamovirus turnip vein-clearing virus (tvcv), itself causing almost imperceptible symptoms in infected turnips, exacerbated symptoms of infection of turnip by the cabbage s isolate of the caulimovirus cauliflower mosaic virus (camv). the synergy in symptom ... | 2002 | 12111413 |
| production of immunogenic vp6 protein of bovine group a rotavirus in transgenic potato plants. | we report here the production of transgenic potato plants expressing the major capsid protein vp6 of bovine group a rotavirus (gar). transgenic plants under the control of a cauliflower mosaic virus 35s promoter, or a modified promoter linked to the tobacco mosaic virus 5'-untranslated sequence were positive for gar antigens by elisa. the expressed protein was consistent in size with vp6 of gar by western blot assay. the presence of the vp6 gene and its transcript was detected by pcr and rt-pcr. ... | 2002 | 12111435 |
| heterologous expression of a bacterial homospermidine synthase gene in transgenic tobacco: effects on the polyamine pathway. | homospermidine synthase (hss) is a branch-point enzyme that links the secondary pathway (pyrrolizidine alkaloids) to primary metabolism (polyamines). since the diamine putrescine is a precursor of homospermidine and nicotine in tobacco, we performed heterologous expression of a bacterial homospermidine synthase gene (hss)in nicotiana tabacum and determined the effect on free and conjugated polyamine levels. the hss gene from rhodopseudomonas viridis was placed under the control of the cauliflowe ... | 2002 | 12112034 |
| plant-specific promoter sequences carry elements that are recognised by the eubacterial transcription machinery. | during evolution the promoter elements from prokaryotes and eukaryotes have developed differently with regard to their sequence and structure, implying that in general a transfer of eukaryotic promoter sequences into prokaryotes will not cause an efficient gene expression. however, there have been reports on the functionality of the 35s promoter from cauliflower mosaic virus (camv) in bacteria. we therefore decided to experimentally investigate the capability of plant promoter sequences to direc ... | 2002 | 12113461 |
| effect of yeast cta1 gene expression on response of tobacco plants to tobacco mosaic virus infection. | the response of tobacco (nicotiana tabacum l. cv xanthi-nc) plants with elevated catalase activity was studied after infection by tobacco mosaic virus (tmv). these plants contain the yeast (saccharomyces cerevisiae) peroxisomal catalase gene cta1 under the control of the cauliflower mosaic virus 35s promoter. the transgenic lines exhibited 2- to 4-fold higher total in vitro catalase activity than untransformed control plants under normal growth conditions. cellular localization of the cta1 prote ... | 2002 | 12114558 |
| heterology expression of the arabidopsis c-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato. | in an attempt to improve stress tolerance of tomato (lycopersicon esculentum) plants, an expression vector containing an arabidopsis c-repeat/dehydration responsive element binding factor 1 (cbf1) cdna driven by a cauliflower mosaic virus 35s promoter was transferred into tomato plants. transgenic expression of cbf1 was proved by northern- and western-blot analyses. the degree of chilling tolerance of transgenic t(1) and t(2) plants was found to be significantly greater than that of wild-type to ... | 2002 | 12114563 |
| potato gene y-1 is an n gene homolog that confers cell death upon infection with potato virus y. | adg2 is a dna sequence mapped to a resistance (r) gene-rich region at the distal end of chromosome xi in potato (solanum tuberosum subsp. andigena). the gene, in which adg2 represents the predicted nucleotide-binding domain (nbs), was cloned and characterized. the coding region of the gene (designated as y-1) is 6,187 bp long and structurally similar to gene n that confers hypersensitive resistance to tobacco mosaic virus in nicotiana spp. both belong to the tir-nbs-lrr class of genes and show 5 ... | 2002 | 12118888 |
| characterization of a stamen-specific cdna encoding a novel plant defensin in chinese cabbage. | we isolated a stamen-specific cdna, bsd1 (brassica stamen specific plant defensin 1) that encodes a novel plant defensin peptide in chinese cabbage (brassica campestris l. ssp. pekinensis). plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. in bsd1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. bsd ... | 2002 | 12139009 |
| molecular breeding of transgenic rice plants expressing a bacterial chlorocatechol dioxygenase gene. | the cbna gene encoding the chlorocatechol dioxygenase gene from ralstonia eutropha nh9 was introduced into rice plants. the cbna gene was expressed in transgenic rice plants under the control of a modified cauliflower mosaic virus 35s promoter. western blot analysis using anti-cbna protein indicated that the cbna gene was expressed in leaf tissue, roots, culms, and seeds. transgenic rice calluses expressing the cbna gene converted 3-chlorocatechol to 2-chloromucote efficiently. growth and morpho ... | 2002 | 12147507 |
| down-regulation of tomato beta-galactosidase 4 results in decreased fruit softening. | transcript abundance of the gene encoding beta-galactosidase ii, a beta-galactosidase/exo-galactanase (ec 3.2.1.23) present during tomato (lycopersicon esculentum) fruit ripening, was suppressed by expression of an antisense tomato beta-galactosidase 4 (tbg4) cdna driven by the cauliflower mosaic virus 35s promoter. rna gel-blot analysis was used to evaluate tbg4 mrna levels in transgenic fruit. all of the antisense lines had attenuated tbg4 mrna levels in turning stage fruit; however, tbg4 mrna ... | 2002 | 12177488 |
| the cauliflower mosaic virus virion-associated protein is dispensable for viral replication in single cells. | cauliflower mosaic virus (camv) open reading frame iii (orf iii) codes for a virion-associated protein (vap), which is one of two viral proteins essential for aphid transmission. however, unlike the aphid transmission factor encoded by camv orf ii, vap is also essential for systemic infection, suggesting that it is a multifunctional protein. to elucidate the additional function or functions of vap, we tested the replication of noninfectious orf iii-defective mutants in transfected turnip protopl ... | 2002 | 12186927 |
| expression of mangrove allene oxide cyclase enhances salt tolerance in escherichia coli, yeast, and tobacco cells. | to analyze the mechanisms of salt tolerance in the mangrove plant, bruguiera sexangula, functional screening for cdnas encoding proteins essential for salt tolerance was performed using escherichia coli as the host organism. a transformant expressing a protein homologous to lycopersicon (tomato) allene oxide cyclase (aoc) displayed enhanced salt tolerance. however, this unusual trait is not conferred by lycopersicon aoc or its arabidopsis homolog. analysis of the functional region revealed a seq ... | 2002 | 12198193 |
| third position codon composition suggests two classes of genes within the cauliflower mosaic virus genome. | the translation of viral mrnas by host ribosomes is essential for infection. hence, codon usage of virus genes may influence efficiency of infection. in addition, composition of nucleotides in the third position within codons of genes can reflect evolutionary relationships. in this study, third position codon composition was examined for the seven genes of eight cauliflower mosaic virus isolates. genes iv-vii had similar codon composition values and were termed class 1 genes. genes i-iii possess ... | 2002 | 12202113 |
| reduction of uroporphyrinogen decarboxylase by antisense rna expression affects activities of other enzymes involved in tetrapyrrole biosynthesis and leads to light-dependent necrosis. | we introduced a full-length cdna sequence encoding tobacco (nicotiana tabacum) uroporphyrinogen iii decarboxylase (urod; ec 4.1.1.37) in reverse orientation under the control of a cauliflower mosaic virus 35s promoter derivative into the tobacco genome to study the effects of deregulated urod expression on tetrapyrrole biosynthesis. transformants with reduced urod activity were characterized by stunted plant growth and necrotic leaf lesions. antisense rna expression caused reduced urod protein l ... | 1997 | 12223662 |
| nadp-malate dehydrogenase in the c4 plant flaveria bidentis (cosense suppression of activity in mesophyll and bundle-sheath cells and consequences for photosynthesis). | flaveria bidentis, a c4 dicot, was transformed with sorghum (a monocot) cdna clones encoding nadp-malate dehydrogenase (nadp-mdh; ec 1.1.1.82) driven by the cauliflower mosaic virus 35s promoter. although these constructs were designed for over-expression, many transformants contained between 5 and 50% of normal nadp-mdh activity, presumably by cosense suppression of the native gene. the activities of a range of other photosynthetic enzymes were unaffected. rates of photosynthesis in plants with ... | 1997 | 12223666 |
| insect control and dosage effects in transgenic canola containing a synthetic bacillus thuringiensis cryiac gene. | zygotic hypocotyls of canola (brassica napus l.) cv oscar, cv westar, and the breeding line uga188-20b were transformed with a truncated synthetic bacillus thuringiensis insecticidal crystal protein gene (bt cryiac) under the control of the cauliflower mosaic virus 35s promoter using agrobacterium tumefaciens-mediated transformation. fifty-seven independently transformed lines were produced, containing 1 to 12 copies of the transgenes. a range of cry expressors was produced from 0 to 0.4% cry as ... | 1996 | 12226379 |
| expression of the agrobacterium rhizogenes rolc gene in a deciduous forest tree alters growth and development and leads to stem fasciation. | we have altered the growth and development of a deciduous forest tree by transforming hybrid aspen (populus tremula x populus tremuloides) with the agrobacterium rhizogenes rolc gene expressed under the strong cauliflower mosaic virus 35s promoter. we demonstrate that the genetically manipulated perennial plants, after a period of dormancy, maintain the induced phenotypical changes during the second growing period. furthermore, mass-spectrometrical quantifications of the free and conjugated form ... | 1996 | 12226405 |
| the tomato e8 gene influences ethylene biosynthesis in fruit but not in flowers. | we investigated the function of the tomato (lycopersicon esculentum) e8 gene. previous experiments in which antisense suppression of e8 was used suggested that the e8 protein has a negative effect on ethylene evolution in fruit. e8 is expressed in flowers as well as in fruit, and its expression is high in anthers. we introduced a cauliflower mosaic virus 35s-e8 gene into tomato plants and obtained plants with overexpression of e8 and plants in which e8 expression was suppressed due to co-suppres ... | 1996 | 12226407 |
| accumulation of 15-kilodalton zein in novel protein bodies in transgenic tobacco. | zeins, the seed storage proteins of maize, are a group of alcohol-soluble polypeptides of different molecular masses that share a similar amino acid composition but vary in their sulfur amino acid composition. they are synthesized on the rough endoplasmic reticulum (er) in the endosperm and are stored in er-derived protein bodies. our goal is to balance the amino acid composition of the methionine-deficient forage legumes by expressing the sulfur amino acid-rich 15-kd zeins in their leaves. howe ... | 1995 | 12228338 |
| interspecific gene transfer (implications for broadening temperature characteristics of plant metabolic processes). | we report here an approach to metabolic engineering to alter the temperature characteristics of an enzyme pool based on the concept of thermal kinetics windows (tkws), a useful indicator of enzyme performance. a chimeric cucumber nadh-hydroxypyruvate reductase (hpr) gene under the control of a cauliflower mosaic virus 35s promoter was constructed and introduced into the genome of tobacco (tobacum tobacum). the root system of the r1 generation of the resultant transgenic plants expresses only the ... | 1995 | 12228369 |
| repression of acetolactate synthase activity through antisense inhibition (molecular and biochemical analysis of transgenic potato (solanum tuberosum l. cv desiree) plants). | acetolactate synthase (als), the first enzyme in the biosynthetic pathway of leucine, valine, and isoleucine, is the biochemical target of different herbicides. to investigate the effects of repression of als activity through antisense gene expression we cloned an als gene from potato (solanum tuberosum l. cv desiree), constructed a chimeric antisense gene under control of the cauliflower mosaic virus 35s promoter, and created transgenic potato plants through agrobacterium tumefaciens-mediated g ... | 1995 | 12228373 |
| impaired wound induction of 3-deoxy-d-arabino-heptulosonate-7-phosphate (dahp) synthase and altered stem development in transgenic potato plants expressing a dahp synthase antisense construct. | potato (solanum tuberosum l.) cells were transformed with an antisense dna construct encoding part of 3-deoxy-d-arabino-heptulosonate-7-phosphate (dahp) synthase (ec 4.1.2.15), the first enzyme of the shikimate pathway, to examine the role(s) of this protein in plant growth and development. chimeric dna constructs contained the transcript start site, the first exon, and part of the first intron of the shka gene in antisense or sense orientations under the control of the cauliflower mosaic virus ... | 1995 | 12228551 |
| increased putrescine biosynthesis through transfer of mouse ornithine decarboxylase cdna in carrot promotes somatic embryogenesis. | carrot (daucus carota l.) cells were transformed with agrobacterium tumefaciens strains containing 3[prime]-truncated mouse ornithine decarboxylase (odc) cdna under the control of a cauliflower mosaic virus 35s promoter. a neomycin phosphotransferase gene linked with a nopaline synthase promoter was used to select transformed cell lines on kanamycin. although the nontransformed cells contained no odc, high amounts of mouse-specific odc activity were observed in the transformed cells. transgenic ... | 1995 | 12228581 |
| transformation and regeneration of two cultivars of pea (pisum sativum l.). | a reproducible transformation system was developed for pea (pisum sativum l.) using as explants sections from the embryonic axis of immature seeds. a construct containing two chimeric genes, nopaline synthase-phosphinothricin acetyl transferase (bar) and cauliflower mosaic virus 35s-neomycin phosphotransferase (nptii), was introduced into two pea cultivars using agrobacterium tumefaciens-mediated transformation procedures. regeneration was via organogenesis, and transformed plants were selected ... | 1993 | 12231726 |
| hormonal characterization of transgenic tobacco plants expressing the rolc gene of agrobacterium rhizogenes tl-dna. | transgenic tobacco (nicotiana tabacum l. cv wisconsin 38) plants expressing the agrobacterium rhizogenes rolc gene under the control of the cauliflower mosaic virus 35s rna promoter were constructed. these plants displayed several morphological alterations reminiscent of changes in indole-3-acetic acid (iaa), cytokinin, and gibberellin (ga) content. however, investigations showed that neither the iaa pool size nor its rate of turnover were altered significantly in the rolc plants. the biggest di ... | 1993 | 12231827 |
| ectopic overexpression of asparagine synthetase in transgenic tobacco. | here, we monitor the effects of ectopic overexpression of genes for pea asparagine synthetase (as1) in transgenic tobacco (nicotiana tabacum). the as genes of pea and tobacco are normally expressed only during the dark phase of the diurnal growth cycle and specifically in phloem cells. a hybrid gene was constructed in which a pea as1 cdna was fused to the cauliflower mosaic virus 35s promoter. the 35s-as1 gene was therefore ectopically expressed in all cell types in transgenic tobacco and consti ... | 1993 | 12232020 |
| the rolb gene of agrobacterium rhizogenes does not increase the auxin sensitivity of tobacco protoplasts by modifying the intracellular auxin concentration. | phenotypical alterations observed in rolb-transformed plants have been proposed to result from a rise in intracellular free auxin due to a rolb-catalyzed hydrolysis of auxin conjugates(j.j. estruch, j. schell, a. spena [1991] embo j 10: 3125-3128).we have investigated this hypothesis in detail using tobacco (nicotiana tabacum) mesophyll protoplasts isolated from plants transformed with the rolb gene under the control of its own promoter (bbgus 6 clone) or the cauliflower mosaic virus 35s promote ... | 1994 | 12232224 |
| metabolic control of anaerobic glycolysis (overexpression of lactate dehydrogenase in transgenic tomato roots supports the davies-roberts hypothesis and points to a critical role for lactate secretion. | roots of all plants examined so far have the potential for both ethanol and lactate fermentation. a short burst of lactate fermentation usually occurs when plant tissues are transferred from normoxic to anoxic conditions. according to the davies-roberts hypothesis, the consequent ph drop both initiates ethanol fermentation and blocks further production of lactate by inhibiting lactate dehydrogenase (ldh). however, the role of ldh in this ph control mechanism is still a matter of debate. to pertu ... | 1994 | 12232401 |
| the frequency and degree of cosuppression by sense chalcone synthase transgenes are dependent on transgene promoter strength and are reduced by premature nonsense codons in the transgene coding sequence. | by comparing the effects of strong and weak promoters that drive sense chalcone synthase (chs) transgenes in large populations of independently transformed plants, we show here that a strong transgene promoter is required for high-frequency cosuppression of chs genes and for production of the full range of cosuppression phenotypes. in addition, sense chs transgenes driven by a cauliflower mosaic virus 35s promoter possessing a single copy of the upstream activator region (uar) were found to prod ... | 1997 | 12237385 |
| a transcriptionally active state is required for post-transcriptional silencing (cosuppression) of nitrate reductase host genes and transgenes. | using tobacco nitrate reductase cosuppression as a model system of post-transcriptional gene silencing, we analyzed the influence of dna and rna dosages both together and independently. for this purpose, zero, one, two, or four active or transcriptionally silenced copies of a cauliflower mosaic virus 35s-nia2 transgene were combined by transformation and subsequent crosses with zero, one, two, three, or four active, disrupted, or transcriptionally repressed copies of the wild-type host nia genes ... | 1997 | 12237392 |
| approaching the lower limits of transgene variability. | the inclusion of chicken lysozyme matrix-associated regions (mars) in t-dna has been demonstrated to reduce the variation in [beta]-glucuronidase (gus) gene expression among first-generation transformed plants. the residual variation observed between transgenic plant lines with mars at the t-dna borders was investigated. by definition, any phenotypic variance between or within genetically identical plants is caused by random or environmental variation. this variation therefore sets a lower limit ... | 1996 | 12239419 |
| expression of a flax allene oxide synthase cdna leads to increased endogenous jasmonic acid (ja) levels in transgenic potato plants but not to a corresponding activation of ja-responding genes. | both jasmonic acid (ja) and its methyl ester, methyl jasmonate (meja), are thought to be significant components of the signaling pathway regulating the expression of plant defense genes in response to various stresses. ja and meja are plant lipid derivatives synthesized from [alpha]-linolenic acid by a lipoxygenase-mediated oxygenation leading to 13-hydroperoxylinolenic acid, which is subsequently transformed by the action of allene oxide synthase (aos) and additional modification steps. aos con ... | 1995 | 12242357 |
| the mar-mediated reduction in position effect can be uncoupled from copy number-dependent expression in transgenic plants. | to study the role of matrix-associated regions (mars) in establishing independent chromatin domains in plants, two transgenes were cloned between chicken lysozyme a elements. these transgenes were the neomycin phosphotransferase (nptii) gene under control of the nopaline synthase (nos) promoter and the [beta]-glucuronidase (gus) gene controlled by the double cauliflower mosaic virus (dcamv) 35s promoter. the a elements are supposed to establish an artificial chromatin domain upon integration int ... | 1995 | 12242378 |
| fructan as a new carbohydrate sink in transgenic potato plants. | fructans are polyfructose molecules that function as nonstructural storage carbohydrates in several plant species that are important crops. we have been studying plants for their ability to synthesize and degrade fructans to determine if this ability is advantageous. we have also been analyzing the ability to synthesize fructan in relation to other nonstructural carbohydrate storage forms like starch. to study this, we induced fructan accumulation in normally non-fructan-storing plants and analy ... | 1994 | 12244248 |
| tissue-specific expression of as-1 in transgenic tobacco. | when integrated as a transgene in one or a few copies, the -90 35s promoter of cauliflower mosaic virus confers expression in roots with little or no expression in cotyledons and leaves. the responsible cis element, activation sequence-1 (as-1), can bind to the nuclear factor asf-1 as well as to the transcription factor tga1a. here, we show that microinjection of 104 molecules of tga1a per cotyledon cell activated transgenes containing as-1-linked promoters. transgenes with promoters linked to t ... | 1994 | 12244260 |
| agroinfection as a rapid method for propagating cowpea mosaic virus-based constructs. | to increase the efficiency of infections with cowpea mosaic virus (cpmv)-based constructs, clones suitable for agroinfection were constructed. full-length copies of rna-1 and rna-2 were inserted between the sequence of a cauliflower mosaic virus (camv) 35s promoter and a nos terminator and were introduced into the agrobacterium tumefaciens plasmid, pbinplus. infiltration of leaves of either nicotiana benthamiana or cowpea (vigna unguiculata) with a bacterial suspension containing a mixture of th ... | 2002 | 12270666 |
| expansion of viral host range through complementation and recombination in transgenic plants. | we have shown previously that gene vi of cauliflower mosaic virus (camv) strain d4 governs systemic infection of nicotiana bigelovii and that transgenic n. bigelovii expressing the d4 gene vi product can complement at least one camv isolate for long-distance transport. we have now found that dna of two other isolates of camv recombine with the gene vi coding sequence present in the transgenic plants. the formation of recombinant viruses occurs as a consequence of camv replication, involving two ... | 1993 | 12271051 |
| cauliflower mosaic virus gene vi controls translation from dicistronic expression units in transgenic arabidopsis plants. | transformed arabidopsis plants were used to study the effect of the cauliflower mosaic virus (camv) inclusion body protein on translation of dicistronic rna. reporter plants contain a dicistronic transcription unit with camv open reading frame vii (orf vii) as the first and the [beta]-glucuronidase (gus) reporter orf as the second cistron. "transactivator plants" contain camv orf vi under the control of the strong camv 35s promoter. the transactivator plants were difficult to regenerate and show ... | 1992 | 12297640 |
| expression of a self-incompatibility glycoprotein (s2-ribonuclease) from nicotiana alata in transgenic nicotiana tabacum. | in nicotiana alata, self-incompatibility is controlled by a single locus, designated the s-locus, with multiple alleles. stylar products of these alleles are ribonucleases that are secreted mainly in the transmitting tract tissues. n. tabacum plants were transformed with constructs containing the s2-cdna and genomic s2-sequences from n. alata that were linked to the cauliflower mosaic virus 35s promoter. unlike other genes controlled by this promoter, the genes were expressed most highly in matu ... | 1992 | 12297668 |
| overexpression of phytochrome b induces a short hypocotyl phenotype in transgenic arabidopsis. | the photoreceptor phytochrome is encoded by a small multigene family in higher plants. phya encodes the well-characterized etiolated-tissue phytochrome. the product of the phyb gene, which has properties resembling those of "green tissue" phytochrome, is as yet poorly characterized. we have developed a phytochrome b overexpression system for analysis of the structure and function of this protein. using newly generated polyclonal and monoclonal antibodies that are selective for phytochrome b, we ... | 1991 | 12324591 |
| peroxidase-induced wilting in transgenic tobacco plants. | peroxidases are a family of isoenzymes found in all higher plants. however, little is known concerning their role in growth, development, or response to stress. plant peroxidases are heme-containing monomeric glycoproteins that utilize either h2o2 or o2 to oxidize a wide variety of molecules. to obtain more information on possible in planta functions of peroxidases, we have used a cdna clone for the primary isoenzyme form of peroxidase to synthesize high levels of this enzyme in transgenic plant ... | 1990 | 12354942 |
| localization of elements important for the wound-inducible expression of a chimeric potato proteinase inhibitor ii-cat gene in transgenic tobacco plants. | the effect of progressive 5[prime] deletions within a potato proteinase inhibitor ii promoter on wound-inducible expression of the chloramphenicol acetyltransferase (cat) gene in leaves of transgenic tobacco plants was analyzed. after deletion of a region ranging from position -1300 to -700 with respect to the transcription start site, promoter activity was markedly reduced but still wound-inducible. further deletion of approximately 200 base pairs resulted in a promoter activity that was below ... | 1990 | 12354945 |
| cis-acting elements for light regulation of pea ferredoxin i gene expression are located within transcribed sequences. | an intact pea gene encoding ferredoxin i (fed-1) and several chimeric constructs containing portions of fed-1 were introduced into tobacco plants by agrobacterium-mediated transformation. the intact gene was correctly transcribed and translated to produce a protein that was imported into the chloroplast and processed to its mature size. fed-1 mrna accumulation in these plants was strongly light-dependent, as it is in pea leaves. in chimeric constructs, the fed-1 promoter was active but no light ... | 1989 | 12359905 |
| oat phytochrome is biologically active in transgenic tomatoes. | to determine the functional homology between phytochromes from evolutionarily divergent species, we used the cauliflower mosaic virus 35s promoter to express a monocot (oat) phytochrome cdna in a dicot plant (tomato). immunoblot analysis shows that more than 50% of the transgenic tomato plants synthesize the full-length oat phytochrome polypeptide. moreover, leaves of light-grown transgenic plants contain appreciably less oat phytochrome than leaves from dark-adapted plants, and etiolated r1 tra ... | 1989 | 12359910 |
| rice phytochrome is biologically active in transgenic tobacco. | to investigate the mechanisms of phytochrome action in vivo, we have overexpressed rice phytochrome in transgenic tobacco plants. a full-length rice phytochrome cdna was fused to the cauliflower mosaic virus 35s promoter and transferred to tobacco. the progeny of some of the transgenic plants contain large amounts of rice phytochrome mrna in green leaves. extracts prepared from overexpressing plants contain twofold to fivefold more spectrophotometrically detectable phytochrome than extracts from ... | 1989 | 12359911 |
| over-expression of a seed specific hevein-like antimicrobial peptide from pharbitis nil enhances resistance to a fungal pathogen in transgenic tobacco plants. | two hevein-like peptides from the seed of pharbitis nil, designated pharbitis nil antimicrobial peptide 1 (pn-amp1) and pn-amp2, had been purified previously. both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. we now report the isolation of two cdna clones, designated pnamp-h1 and pnamp-h2, and the corresponding genomic clones encoding these proteins from mature seeds of p. nil. comparison of the deduced amino acid sequence to that of the mature p ... | 2002 | 12369620 |
| powerful effect of an atypical bifactorial endosperm box from wheat hmwg-dx5 promoter in maize endosperm. | the proximal region of the high-molecular-weight glutenin promoter of the dx5 gene (prhmwg-dx5) carries an atypical bifactorial endosperm box containing two cis-acting elements, namely a g-box like motif followed by a prolamin-box motif (pb1). transient expression assays in maize endosperm indicate that a promoter fragment containing at least the g-box like element is necessary and sufficient for maximal expression of the hmwg-dx5 promoter. in transformed maize, we have shown that a 89 bp sequen ... | 2002 | 12374301 |
| novel reference molecules for quantitation of genetically modified maize and soybean. | new quantitation methods based on a real-time polymerase chain reaction (pcr) technique were developed for 5 lines of genetically modified (gm) maize, including mon810, event176, bt11, t25, and ga21, and a gm soy, roundup ready. oligonucleotide dna, including specific primers and fluorescent dye-labeled probes, were designed for pcrs. two plasmids were constructed as reference molecules (rms) for the detection of gm maize and gm soy. the molecules contain the dna sequences of a specific region f ... | 2015 | 12374407 |
| tomato plants ectopically expressing arabidopsis cbf1 show enhanced resistance to water deficit stress. | a dna cassette containing an arabidopsis c repeat/dehydration-responsive element binding factor 1 (cbf1) cdna and a nos terminator, driven by a cauliflower mosaic virus 35s promoter, was transformed into the tomato (lycopersicon esculentum) genome. these transgenic tomato plants were more resistant to water deficit stress than the wild-type plants. the transgenic plants exhibited growth retardation by showing dwarf phenotype, and the fruit and seed numbers and fresh weight of the transgenic toma ... | 2002 | 12376629 |
| mutation of capsid protein phosphorylation sites abolishes cauliflower mosaic virus infectivity. | the cauliflower mosaic virus (camv) capsid protein is derived by bidirectional processing of the precapsid protein (cp56). we expressed several derivatives of cp56 in escherichia coli and used them as substrates for virus-associated kinase and casein kinase ii purified from plant cells. three serine residues located at the n terminus of the mature viral protein cp44 were identified as phosphorylation targets. a mutation of one of them in the viral context had little or no effect on viral infecti ... | 2002 | 12388736 |
| boosting heterologous protein production in transgenic dicotyledonous seeds using phaseolus vulgaris regulatory sequences. | over the past decade, several high value proteins have been produced in different transgenic plant tissues such as leaves, tubers, and seeds. despite recent advances, many heterologous proteins accumulate to low concentrations, and the optimization of expression cassettes to make in planta production and purification economically feasible remains critical. here, the regulatory sequences of the seed storage protein gene arcelin 5-i (arc5-i) of common bean (phaseolus vulgaris) were evaluated for p ... | 2002 | 12415287 |
| generation of sirnas by t-dna sequences does not require active transcription or homology to sequences in the plant. | delivery into plants of t-dnas containing promoter, terminator, or coding sequences generated small interfering rnas (sirnas) specific to each type of sequence. when both promoter and transcribed sequences were simultaneously present in the t-dna, accumulation of sirnas to transcribed sequences was favored over accumulation of sirnas to the nontranscribed upstream promoter sequences. the generation of specific sirna sequences occurred even in the absence of t-dna homology to sequences in the pla ... | 2002 | 12423019 |
| the systemin precursor gene regulates both defensive and developmental genes in solanum tuberosum. | transformation of solanum tuberosum, cv. desiree, with the tomato prosystemin gene, regulated by the 35s cauliflower mosaic virus promoter, resulted in constitutive increase in defensive proteins in potato leaves, similar to its effects in tomato plants, but also resulted in a dramatic increase in storage protein levels in potato tubers. tubers from selected transformed lines contained 4- to 5-fold increases in proteinase inhibitor i and ii proteins, >50% more soluble and dry weight protein, and ... | 2002 | 12426402 |
| functional analysis of regulatory elements in the gene promoter for an abscission-specific cellulase from bean and isolation, expression, and binding affinity of three tga-type basic leucine zipper transcription factors. | site-directed mutagenesis was used to identify cis-acting elements that control hormonal and abscission-specific expression of the bean (phaseolus vulgaris) abscission cellulase (bac) promoter. auxin inhibition of bac promoter expression is at least in part controlled by a negatively regulated element and ethylene induction by a positively regulated element. one of a series of 15 different 10-bp mutations created in a 2.9-kb bac promoter reduced reporter gene expression by 60%. the native sequen ... | 2002 | 12428013 |
| an amphibian antimicrobial peptide variant expressed in nicotiana tabacum confers resistance to phytopathogens. | esculentin-1 is a 46-residue antimicrobial peptide present in skin secretions of rana esculenta. it is effective against a wide variety of micro-organisms, including plant pathogens with negligible effects on eukaryotic cells. as a possible approach to enhance plant resistance, a dna coding for esculentin-1, with the substitution met-28leu, was fused at the c-terminal end of the leader sequence of endopolygalacturonase-inhibiting protein, under the control of the cauliflower mosaic virus 35s pro ... | 2003 | 12435273 |
| multiple domains within the cauliflower mosaic virus gene vi product interact with the full-length protein. | the cauliflower mosaic virus (camv) gene vi product (p6) is a multifunctional protein essential for viral propagation. it is likely that at least some of these functions require p6 self-association. the work described here was performed to confirm that p6 self-associates and to identify domains involved in this interaction. yeast two-hybrid analyses indicated that full-length p6 self-associates and that this interaction is specific. additional analyses indicated that at least four independent do ... | 2002 | 12437303 |
| analysis of cis-sequence of subgenomic transcript promoter from the figwort mosaic virus and comparison of promoter activity with the cauliflower mosaic virus promoters in monocot and dicot cells. | a sub-genomic transcript (sgt) promoter was isolated from the figwort mosaic virus (fmv) genomic clone. the fmv sgt promoter was linked to heterologous coding sequences to form a chimeric gene construct. the 5'-3'-boundaries required for maximal activity and involvement of cis-sequences for optimal expression in plants were defined by 5'-, 3'-end deletion and internal deletion analysis of fmv sgt promoter fragments coupled with a beta-glucuronidase reporter gene in both transient protoplast expr ... | 2002 | 12457962 |
| differential tissue-specific response to sulfate and methionine of a soybean seed storage protein promoter region in transgenic arabidopsis. | expression of the gene encoding the beta subunit of beta-conglycinin, a major soybean seed storage protein, is upregulated by sulfur deficiency and downregulated by methionine (met). the tissue-specificity of these regulatory mechanisms was studied using a sulfate-responsive region (beta(sr)) from the beta subunit gene promoter. transgenic arabidopsis thaliana lines were generated carrying a green fluorescent protein (gfp) reporter gene under control of the cauliflower mosaic virus 35s rna promo ... | 2002 | 12461126 |
| delta 12-oleate desaturase-related enzymes associated with formation of conjugated trans-delta 11, cis-delta 13 double bonds. | conjugated linolenic acids are present as major seed oils in several plant species. punicic acid (or trichosanic acid) is a conjugated linolenic acid isomer containing cis-delta9, trans-delta11, cis-delta13 double bonds in the c(18) carbon chain. here we report cdnas, tkfac and pgfac, isolated from trichosanthes kirilowii and punica granatum, that encode a class of conjugases associated with the formation of trans-delta11, cis-delta13 double bonds. expression of tkfac and pgfac in arabidopsis se ... | 2003 | 12464604 |
| cauliflower mosaic virus is preferentially acquired from the phloem by its aphid vectors. | cauliflower mosaic virus (camv) is transmitted in a non-circulative manner by aphids following the helper strategy. helper proteins p2 and p3 act as a bridge between virions and the aphid cuticle. electronic monitoring of aphid stylet activities (epg technique), transmission tests and electron microscopy showed that camv is preferentially acquired from the phloem by its most common aphid vectors, brevycorine brassicae and myzus persicae. we also found that camv is semipersistently transmitted an ... | 2002 | 12466494 |
| differential regulation of tga transcription factors by post-transcriptional control. | transcription factors often belong to multigene families and their individual contribution in a particular regulatory network remains difficult to assess. we show here that specific members from a family of conserved arabidopsis bzip transcription factors, the tga proteins, are regulated in their protein stability by developmental stage-specific proteolysis. using gfp fusions of three different arabidopsis tga factors that represent members of distinct subclasses of the tga factor family, we dem ... | 2002 | 12472682 |
| t-dna insertional mutagenesis for activation tagging in rice. | we have developed a new t-dna vector, pga2715, which can be used for promoter trapping and activation tagging of rice (oryza sativa) genes. the binary vector contains the promoterless beta-glucuronidase (gus) reporter gene next to the right border. in addition, the multimerized transcriptional enhancers from the cauliflower mosaic virus 35s promoter are located next to the left border. a total of 13,450 t-dna insertional lines have been generated using pga2715. histochemical gus assays have reve ... | 2002 | 12481047 |
| geminivirus vectors for high-level expression of foreign proteins in plant cells. | bean yellow dwarf virus (beydv) is a monopartite geminivirus that can infect dicotyledonous plants. we have developed a high-level expression system that utilizes elements of the replication machinery of this single-stranded dna virus. the replication initiator protein (rep) mediates release and replication of a replicon from a dna construct ("lsl vector") that contains an expression cassette for a gene of interest flanked by cis-acting elements of the virus. we used tobacco nt1 cells and biolis ... | 2003 | 12491528 |
| molecular characterization of two plant bi-1 homologues which suppress bax-induced apoptosis in human 293 cells. | to date, few homologues of animal programmed cell death (pcd) regulators have been identified in plants. among these is the plant bax inhibitor-1 (bi-1) protein, which possesses, like its human counterpart, the ability to suppress bax-induced lethality in yeast cells. as the role of bi-1 in the regulation of plant pcd remains to be elucidated, we cloned bnbi-1 and ntbi-1 from cdna libraries of oilseed rape ( brassica napus l.) and tobacco ( nicotiana tabacum l.). the analysis of the deduced amin ... | 2003 | 12520328 |
| [expression of otsa gene in tobacco and improvement stress tolerance]. | the escherichia coli trehalose-6-phosphate synthase gene(otsa) was engineered under the control of cauliflower mosaic virus regulatory sequences (camv35s) for expression in plants. otsa gene was incorporated into the chromosome dna by agrobacterium-mediated transfer and expressed in nicotiana tabacum. the otsa gene transgenic plant exhibited multiple phenotypic alteration: improved stunted growth and drought tolerance. the detached leaves from the transgenic plant, their water loss slower than t ... | 2001 | 12552907 |
| transgenic loblolly pine (pinus taeda l.) plants expressing a modified delta-endotoxin gene of bacillus thuringiensis with enhanced resistance to dendrolimus punctatus walker and crypyothelea formosicola staud. | a synthetic version of the cry1ac gene of bacillus thuringiensis has been used for the transformation of loblolly pine (pinus taeda l.) using particle bombardment. mature zygotic embryos were used to be bombarded and to generate organogenic callus and transgenic regenerated plants. expression vector pb48.215 dna contained a synthetic bacillus thuringiensis (b.t.) cry1ac coding sequence flanked by the double cauliflower mosaic virus (camv) 35s promoter and nopaline synthase (nos) terminator seque ... | 2003 | 12554726 |
| differential induction of symptoms in arabidopsis by p6 of cauliflower mosaic virus. | the gene vi protein (p6) of cauliflower mosaic virus (camv) functions as a virulence factor in crucifers by eliciting chlorotic symptoms in infected plants. the ability to induce chlorosis has been associated previously with p6 through gene-swapping experiments between strains and through the development of transgenic plants that express p6. the primary role that has been identified for p6 in the camv infection cycle is to modify the host translation machinery to facilitate the translation of th ... | 2003 | 12580280 |
| wvd2 and wdl1 modulate helical organ growth and anisotropic cell expansion in arabidopsis. | wild-type arabidopsis roots develop a wavy pattern of growth on tilted agar surfaces. for many arabidopsis ecotypes, roots also grow askew on such surfaces, typically slanting to the right of the gravity vector. we identified a mutant, wvd2-1, that displays suppressed root waving and leftward root slanting under these conditions. these phenotypes arise from transcriptional activation of the novel wave-dampened2 (wvd2) gene by the cauliflower mosaic virus 35s promoter in mutant plants. seedlings ... | 2003 | 12586874 |
| disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. | mitogen-activated protein kinase (mapk) cascades play an important role in mediating stress responses in eukaryotic organisms. however, little is known about the role of mapks in modulating the interaction of defense pathways activated by biotic and abiotic factors. in this study, we have isolated and functionally characterized a stress-responsive mapk gene (osmapk5) from rice. osmapk5 is a single-copy gene but can generate at least two differentially spliced transcripts. the osmapk5 gene, its p ... | 2003 | 12615946 |
| activation of three pathogen-inducible promoters of tobacco in transgenic pear (pyrus communis l.) after abiotic and biotic elicitation. | in order to improve pear resistance against fire blight caused by erwinia amylovora, a search for promoters driving high-level expression of transgenes specifically in response to this bacterial pathogen has been undertaken. we have examined the ability of hsr203j, str246c and sgd24 tobacco (nicotiana tabacum l.) promoters to drive expression of the uida reporter gene in pear. transgenic pear clones were obtained by agrobacterium tumefaciens-mediated transformation. beta-glucuronidase activity w ... | 2003 | 12624768 |
| the promoter of a rice glycine-rich protein gene, osgrp-2, confers vascular-specific expression in transgenic plants. | the genomic sequence of a rice (oryza sativa l.) glycine-rich protein (grp) gene, designated osgrp-2, has been previously determined (genbank u40708). primer extension analysis indicated that transcription starts 47 bp upstream of the translation start codon. to gain an insight into the transcriptional regulation of this gene, the 2,401-bp promoter sequence and a series of its 5' deletions were transcriptionally fused to the beta-glucuronidase (gus) gene. gus activity was subsequently assayed in ... | 2003 | 12624770 |
| [analysis of transgenic tobacco plants carrying the gene for the surface antigen of the hepatitis b virus]. | the plasmids carrying the gene encoding the hepatitis b surface antigen (hbsag) under the control of 35s rna single or dual promoters of the cauliflower mosaic virus camv 35s were constructed. these constructions were used for obtaining transgenic tobacco plants that synthesize the hbs antigen. the presence of hbsag in tobacco plant extracts was confirmed by the enzyme-linked immunoassay using antibodies against the native hbs antigen. the antigen amount in plants carrying the hbsag gene under a ... | 2003 | 12624933 |
| pto mutants differentially activate prf-dependent, avrpto-independent resistance and gene-for-gene resistance. | pto confers disease resistance to pseudomonas syringae pv tomato carrying the cognate avrpto gene. overexpression of pto under the cauliflower mosaic virus 35s promoter activates spontaneous lesions and confers disease resistance in tomato (lycopersicon esculentum) plants in the absence of avrpto. here, we show that these avrpto-independent defenses require a functional prf gene. several pto-interacting (pti) proteins are thought to play a role in pto-mediated defense pathways. to test if intera ... | 2003 | 12644674 |
| csagp1, a gibberellin-responsive gene from cucumber hypocotyls, encodes a classical arabinogalactan protein and is involved in stem elongation. | fluorescence differential display was used to isolate the gibberellin (ga)-responsive gene, csagp1, from cucumber (cucumis sativus) hypocotyls. a sequence analysis of csagp1 indicated that the gene putatively encodes a "classical" arabinogalactan protein (agp) in cucumber. transgenic tobacco (nicotiana tabacum) plants overexpressing csagp1 under the control of the cauliflower mosaic virus 35s promoter produced a y(betaglc)(3)-reactive proteoglycan in addition to agps present in wild-type tobacco ... | 2003 | 12644694 |
| type-1 ribosome-inactivating protein from iris bulbs: a useful agronomic tool to engineer virus resistance? | to study the in planta antiviral activity of a type-1 ribosome-inactivating protein from iris bulbs, called irip, nicotiana tabacum cv. samsun nn was transformed with the irip sequence expressed under the control of the 35s cauliflower mosaic virus promoter. molecular analysis of the transgenic plants and characterization of the purified protein revealed that the recombinant irip from tobacco leaves has the same molecular structure and rna n-glycosidase activity as the native protein from iris b ... | 2003 | 12650622 |
| ozone-induced gene expression occurs via ethylene-dependent and -independent signalling. | recent studies suggest that ethylene is involved in signalling ozone-induced gene expression. we show here that application of ozone increased glucuronidase (gus) expression of chimeric reporter genes regulated by the promoters of the tobacco class i beta-1,3-glucanases (glb and gln2) and the grapevine resveratrol synthase (vst1) genes in transgenic tobacco leaves. 5'-deletion analysis of the class i beta-1,3-glucanase promoter revealed that ozone-induced gene regulation is mainly mediated by th ... | 2003 | 12650625 |
| matrix attachment regions enhance transcription of a downstream transgene and the accessibility of its promoter region to micrococcal nuclease. | nuclear matrix attachment regions (mars) are thought to influence the expression of flanking genes. in this study, we investigated the activation of genes by tobacco mars that had previously been identified in the 5' region of the basic class i chitinase gene, chns0. in transgenic tobacco cells, a construct consisted of the 35s promoter of cauliflower mosaic virus (camv) fused to a beta-glucuronidase gene (uida) with 5' mar elements was expressed at a 10-fold higher level than a similar construc ... | 2003 | 12678555 |
| molecular breeding of transgenic rice expressing a xylanase domain of the xyna gene from clostridium thermocellum. | the gene encoding the catalytic domain of thermostable xylanase from clostridium thermocellum f1 was expressed in rice plants under the control of a constitutive promoter. the gene encoding xylanase a was modified to encode the catalytic domain of family 11 xylanase without the signal sequence (xyna1), and was introduced into rice plants and expressed under the control of a modified cauliflower mosaic virus 35s promoter. zymogram analysis indicated that the recombinant xylanase was produced in r ... | 2003 | 12684848 |
| the promoter-terminator of chrysanthemum rbcs1 directs very high expression levels in plants. | transgenic plants are increasingly used as production platforms for various proteins, yet protein expression levels in the range of the most abundant plant protein, ribulose-1,5-bisphosphate carboxylase have not yet been achieved by nuclear transformation. suitable gene regulatory 5' and 3' elements are crucial to obtain adequate expression. in this study an abundantly transcribed member (rbcs1) of the ribulose-1,5-bisphosphate carboxylase small-subunit gene family of chrysanthemum (chrysanthemu ... | 2003 | 12687368 |
| sweet pepper ferredoxin-like protein ( pflp) gene as a novel selection marker for orchid transformation. | a novel method for selection of transgenic plants utilizing the sweet pepper ( capsicum annuum l.) ferredoxin-like protein ( pflp) gene as selection marker and erwinia carotovora as the selection agent has been developed. an expression vector containing a pflp cdna driven by a cauliflower mosaic virus 35s promoter was successfully transformed into protocorm-like bodies of oncidium orchid by agrobacterium tumefaciens and particle bombardment, respectively. erwinia carotovora was used as a selecti ... | 2003 | 12721849 |
| overexpression of the sucrose transporter sosut1 in potato results in alterations in leaf carbon partitioning and in tuber metabolism but has little impact on tuber morphology. | the aim of this work was to examine the consequences of the heterologous expression of a spinach ( spinacia oleracea l.) sucrose transporter ( sosut1) in potato ( solanum tuberosum l.). many studies have indicated that reduction of the expression of this class of sucrose transporter has deleterious effects on plant growth and development; however, until now the possibility of improving plant performance by enhancing the expression of this sucrose transporter has not been reported. with this inte ... | 2003 | 12721860 |
| the promoter of the potato chitinase c gene directs expression to epidermal cells. | chitinases are ubiquitous proteins that occur in all plants in multiple isoforms. we have isolated the chtc2 gene encoding an unusual, basic (class i) chitinase from potato ( solanum tuberosum l.). in contrast to other chitinase genes, chtc2 is not activated by infection, but rather constitutively expressed in leaves and stems where it is restricted to epidermal cells. sequence analysis revealed a number of potential regulatory elements in the promoter, but most striking was the presence of a 31 ... | 2003 | 12733075 |
| regioselectivity of glucosylation of caffeic acid by a udp-glucose:glucosyltransferase is maintained in planta. | caffeic acid is a phenylpropanoid playing an important role in the pathways leading to lignin synthesis and the production of a wide variety of secondary metabolites. the compound is also an antioxidant and has potential utility as a general protectant against free radicals. three glucosylated forms of caffeic acid are known to exist: the 3- o - and 4- o -glucosides and the glucose ester. this study describes for the first time a glucosyltransferase [udp-glucose:glucosyltransferase (ugt)] that i ... | 2003 | 12741958 |
| shunting and controlled reinitiation: the encounter of cauliflower mosaic virus with the translational machinery. | 2001 | 12762028 |