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meloidogyne cruciani n. sp. a root-knot nematode from st. croix (u.s. virgin islands) with observations on morphology of this and two other species of the genus.meloidogyne cruciani n. sp. infecting tomato (lycopersicon esculentum mill.) in the u.s. virgin islands is described and illustrated. m. cruciani is distinguished from other species of the genus by having punctations around the anus of the female and by the second-stage juveniles possessing tri-lobed esophageal glands which are longer than most other species, with their posterier end at about 46.4% of the body length. the esophageal glands of the immature and adult females are contained in five ...198219295712
description of the blueberry root-knot nematode, meloidogyne carolinensis n. sp.meloidogyne carolinensis n. sp. is described from cultivated highbush blueberry (cultivars derived from hybrids of vaccinium corymbosum l. and v. lamarckii camp) in north carolina. the perineal pattern of the female has a large cuticular ridge that surrounds the perivulval area, and the excretory pore is near the level of the base of the stylet. the stylet is 15.9 mum long and the knobs gradually merge with the shaft. the head shape and stylet morphology of the male are quite variable. the typic ...198219295713
post-infection development and morphology of meloidogyne cruciani.the development and life stages of meloidogyne cruciani on tomato was studied at 28 c. roots of 2-wk-old 'rutgers' tomato seedlings were exposed to inoculum for 24 h, rinsed, and the seedlings repotted. no major changes in juvenile development were observed prior to 8 days after inoculation. at 11 days the second-stage juvenile had enlarged considerably. the genital primordium had not yet asumed the v-shape characteristic of developing females, but the presence of rectal glands identified the ju ...198219295716
influence of soil temperature on meloidogyne incognita resistant and susceptible cotton, gossypium hirsutum.the degree of resistance by a cotton plant to meloidogyne incognita is affected by soil temperature, particularly in moderately resistant cultivars, the total number of nematodes in the resistant and moderately resistant rools at 35 c was equal to, or greater than, the number in susceptible roots at 20, 25, or 30 c. a shift in numbers to developing and egg-bearing forms of nematodes in the susceptible cultivar as tentperature increased indicates development was affected by temperature rather tha ...198219295718
incidence and distinguishing characteristics of meloidogyne chitwoodi and m. hapla in potato from the northwestern united states.from september 1980 to june 1981, a survey was conducted in the major potato growing regions of northern california, idaho, nevada, oregon. and washington to determine the distribution of meloidogyne chitwoodi and other meloidogyne spp. meloidogyne chitwoodi and m. hapla were the only root-knot nematode species detected parasitizing potato in all the states surveyed. meloidogyne chitwoodi occurred alone in 83% of the samples and m. hapla in 11%, with 6% of all samples containing both species. th ...198219295719
influence of initial population densities of meloidogyne incognita on three chile cultivars.the effects of meloidogyne incognita on the big jim, jalapeno, and new mexico no. 6 chile (capsicum annuum) cultivars were investigated in microplots for two growing seasons. all three cultivars were susceptible to m. incognita and reacted similarly to different initial populations of this nematode. severe stunting and yield suppressions occurred at all initial m. incognita densities tested ranging from 385 to 4,230 eggs and larvae/500 cm(3) soil. regression analysis of the microplot data from a ...198219295720
lectin binding to meloidogyne javanica eggs. 198219295730
influence of potassium and nitrogen fertilization on parasitism by the root-knot nematode meloidogyne javanica.the influence of various c oncentrations of k, nitrogen sources, and inoculation with root-knot nematode meloidogyne javanica were evaluated in tomato plants. increased potassium concentration increased top and root fresh weights of intact plants and fresh weights of excised roots. nitrate-fertilized plants weighed more than plants receiving ammonium independent of the k level in the medium. nematode counts on roots were not affected by nutritional differences in intact or excised roots. in inta ...198219295748
optimum initial inoculum levels for evaluation of resistance in tomato to meloidogyne spp. at two different soil temperatures.the effects of meloidogyne incognita or m. javanica at five initial inoculum levels of 20, 100, 200, 1,000, and 2,000 eggs and infective juveniles per seedling on 'floradade,' 'nemarex,' 'patriot,' and 'pi 129149-2(sib)-5' tomatoes maintained at 25 or 32.5 c were studied. the number of egg masses on roots of the susceptible cultivar floradade was similar for both species of root-knot nematodes at either 2.5 or 32.5 c soil temperatures. at 25 c, very low numbers of egg masses were produced by bot ...198219295749
effect of time of application on the action of foliar sprays of oxamyl on meloidogyne hapla in tomato.foliar sprays containing 3,000 or 4,000 ppm oxamyl applied before inoculation with meloidogyne hapla completely protected tomato plants from intection for up to 36 days but sprays containing 1,000 or 2,000 ppm provided only partial protection. postinoculation sprays were less effective than preinoculation sprays but they decreased the numbers of females and their rate of development and increased the numbers of males. similar amounts of oxamyl applied to the soil as a drench or as granules contr ...198319295771
development of meloidogyne incognita inhibited by digtaria decumbens cv. pangola.population densities of the root-knot nematode, meloidogyne incognita, were lower after 90 days in soil planted to digitgrass (digitaria decumbens cv. pangola) than in soil left fallow or planted to tomato. roots of tomato seedlings interplanted with pangola digitgrass were less galled than were roots of tomato seedlings planted alone. fewer second stage larvae invaded roots of pangola digitgrass than tomato and those that entered the grass roots failed to develop beyond the late second stage.198319295772
effect of soil water potential on survival of meloidogyne javanica in fallow soil.a natural infestation of meloidogyne javanica in an aggregated oxisol declined at an exponential rate when aliquots of the soil were stored for 72 days in polyethylene bags at various soil water potentials (psi). time periods required for reduction in soil infestations by 50% were 2.7, 4.9, 110, 10, and 2.6 days at psi of -0.16, -0.30, -1.1, -15, and -92 bars, respectively. in the wetter soils, at psi of -0.16, -0.30, and -1.1 bars, the predominant stage recovered was the second-stage larva. in ...198319295774
effects of temperature and root leachates on embryogenic development and hatching of meloidogyne chitwoodi and m. hapla.at 20 c the duration of the embryogenic development of meloiclogyne chitwoodi and m. hapla was about 20 days. at 10 c the embryogenic development was 82-84 days for m. chitwoodi and 95-97 days for m. hapla. the effect of distilled water and root leachates of potato cv. russet burbank, tomato cv. columbian, and wheat cv. hyslop on the hatching of eggs of the two root-knot nematode species was investigated at 4, 7, 10, 15, 20, and 25 c (+/- 1 c). cumulative egg taatch was no greater in root leacha ...198319295777
ultrastructural changes caused by fusarium oxysporum f. sp. lycopersici in meloidogyne javanica induced giant cells in fusarium resistant and susceptible tomato cultivars.tomato (lycopersicon esculentum mill.) seedlings, susceptible (cv. pearson a-i improved) and resistant (cv. pearson improved) to race 1 fusarium oxysporum f. sp. lycopersici (sacc.) snyd &hans., were inoculated with meloidogyne javanica (trueb) chitwood second-stage juveniles and 3 weeks later with race 1 f. oxysporum f. sp. lycopersici spores. one week after fungal inoculation, no fungus was visible in root tissue of the tomato cultivars and the giant cells were normal. two weeks after fungal i ...198319295778
effects of concomitant development on reproduction of meloidogyne incognita and rotylenchulus reniformis on sweet potato.the influence of various factors on reproduction of concomitant meloidogyne incognita (mi) and rotylenchulus reniformis (rr) on sweet potato were studied in the greenhouse. reproduction of rr was reduced by mi at all inoculum levels and experiment durations used, while mi reproduction was not inhibited. both species failed to affect each other when inoculated simultaneously onto root systems developed in separate pots from different nodes of the same plant. reproduction of each species was not s ...198319295794
validation of a model for prediction of host damage by two nematode species.plant roots were mechanically injured or subjected to nematode parasitism to test the model of host damage by two nematode species: y = m' + (l - m')c'z(p)z(p) for y </= 1.0 and y = 1.0 for y > 1.0, where m' = m + (m - m) (1 - y)/[(1 - y) + (l - y)] and c' = (z(-t) + z(-t))/2. damage functions for greenhouse-grown radish plants (cv. cherry belle) mechanically injured with small or large steel needles were used to predict growth of plants injured by both needles. growth predictions accounted for ...198319295796
development of the false root-knot nematode, nacobbus aberrans, on sugarbeet.the duration of the embryogenic development of nacobbus aberrans (= n. batatiformis) took 9-10 days at 25 c and 51 days at 15 c. the j molted in the egg; hence the je emerged from the egg. the effect of distilled water attd root leachates of kochia and sugarbeet was investigated at 5, 10, 15, 20, and 25 c. root leachates did not significantly affect the percent of cumulative hatch of eggs, but temperature did significantly affect emergence of juveniles (p = 0.05). less than 1, 5, and 20% of eggs ...198319295804
parasitic variability of meloidogyne incognita populations on susceptible and resistant cotton.root gall induction and egg production by the four recognized host races and two cytological races of meloidogyne incognita were compared on cotton gossypium hirsutum cvs. deltapine 16 (root-knot susceptible) and auburn 634 (highly resistant). the 12 nematode populations included in the study were from various parts of the world. no population increases occurred on the highly resistant cultivar. after 45 days, populations of host races 1 and 2 induced slight root galling on both cuhivars with on ...198319295806
meloidogyne aquatilis n. sp (nematoda:meloidogynidae) from spartina pectinata with a key to the canadian species of meloidogyne.a new root-knot nematode, meloidogyne aquatilis n. sp., attacking the roots of spartina pectinata link growing in the ottawa river is described and illustrated. meloidogyne aquatilis is distinguished from m. graminis by the light brown body color and by the absence of perineal lateral fields in the female. the male differs by the shorter stylet and by the hemizonid being separated by 7-9 annules from the excretory pore. the second-stage juveniles are also recognized by the 7-9-annule gap between ...198319295816
meloidogyne enterolobii n. sp. (meloidogynidae), a root-knot nematode parasitizing pacara earpod tree in china.meloidogyne enterolobii n. sp. is described and illustrated from roots of pacara earpod tree, enterolobium contortisiliquum (vell.) morong, on hainan island in china. the perineal pattern of the female is usually oval shaped, the striae are fine to coarse, the dorsal arch is moderately high to high and usually rounded, and the phasmids are large. the stylet knobs in females are divided longitudinally by a groove so that each knob appears as two. the mean distance of the excretory pore to the ant ...198319295822
influence of maize rotations on the yield of soybean grown in meloidogyne incognita infested soil.a replicated field study was conducted from 1972 to 1980 involving soybeans grown in 2-, 3-, and 4-year rotations with maize in soil infested with meloidogyne incognita. monocultured soybeans were maintained as controls. cropping regimes involved root-knot nematode susceptible and resistant soybean cultivars and soybeans treated and not treated with nematicides. yields of susceptible cultivars declined with reduced length of rotation. nematicide treatment significantly increased yields of suscep ...198319295824
interaction of vesicular-arbuscular mycorrhizal fungi and phosphorus with meloidogyne incognita on tomato.the influence of two vesicular-arbuscular mycorrhizal fungi and phosphorus (p) nutrition on penetration, development, and reproduction by meloidogyne incognita on walter tomato was studied in the greenhouse. inoculation with either gigaspora margarita or glomus mosseae 2 wk prior to nematode inoculation did not alter infection by m. incognita compared with nonmycorrhizal plants, regardless of soil p level (either 3 mug [low p] or 30 mug [high p] available p/g soil). at a given soil p level, nema ...198319295826
effects of dosage sequence on the efficacy of nonfumigant nematicides, plantain yields, and nematode seasonal fluctuations as influenced by rainfall.four nonfumigant nematicides applied three times during the wet season were used to study dosage sequence and nematicide effectiveness. control of helicotylenchus multicinctus (cobb) thorne and meloidogyne javanica (treub) chitwood increased plantain (musa aab) yields. the nematicide (aldicarb, carbofuran, oxamyl, and miral) performance and yield response varied with dosage sequences. applications of 2, 3, and 2 g ai/tree in march, july, and october (sequence i), respectively, gave greater contr ...198319295837
effect of aldicarb, ethoprop, and carbofuran on control of coffee root-knot nematode, meloidogyne exigua.egg hatch of meloidogyne exigua was significantly inhibited in 14 days pretreatment with aldicarb, ethoprop, or carbofnran at concentrations higher than 0.1 mug/ml; these eggs were found to delay hatch in 19 days posttreatment in ethoprop. aldicarb and carbofuran solutions at concentrations greater than 0.1 mug/ml significantly decreased the motility and the life span of the second-stage juveniles; aldicarb was more toxic than carbofuran to the nematode. in a field test, aldicarb (temik 10g), et ...198319295839
studies on lasioseius scapulatus, a mesostigmatid mite predaceous on nematodes.the life history and feeding habits of lasioseius scapulatus, an ascid predator and potential biocontrol agent of nematodes, was examined. reproduction was asexual, and the life cycle was 8-10 days at room temperature. life history consisted of the egg, protonymph, deutonymph, and adult. both nymphal stages and the adult captured and consumed nematodes. two fungal genera and eight genera of nematodes were suitable food sources. second-stage root-knot nematode juveniles were eaten, but eggs and a ...198319295841
interrelationships between ethylene production, gall formation, and root-knot nematode development in tomato plants infected with meloidogyne javanica.ethylene production was determined in excised tomato (lycopersicon esculentum) root cultures of meloidogyne javanica susceptible and resistant cultivars infected with m. javanica. uninfected cultivars produced very low amounts of ethylene. relatively high amounts of ethylene were produced by the infected susceptible cultivars. peak production of 1.6 n moles * g root(1) * h(1) occurred between 9 and 16 days after inoculation (dai). the period of high ethylene production coincided with that of rap ...198319295844
influence of six vegetable cultivars on reproduction of meloidogyne javanica.replicated field and greenhouse experiments were used to evaluate the effect of tomato, cabbage, cucumber, carrot, amaranthus hybridus, and pepper on growth and fecundity of meloidogyne spp., particularly m. javanica. in the field tests, tomato, cucumber, and carrot favored population increases of meloidogyne spp., while amaranthus, pepper, and cabbage limited them. some cropping sequences that included crops from the latter group had a suppressive effect on population growth. thus, of the 36 cr ...198319295847
reproduction of two races of meloidogyne incognita in tomato plants grown at high temperature. 198319295858
inhibitory effect of watermelon mosaic virus on meloidogyne javanica (treub) chitwood infecting cucurbita pepo l. 198419295884
influence of urea, hydroxyurea, and thiourea on meloidogyne javanica and infected excised tomato roots in culture.urea (u), hydroxyurea (hu), and thiourea (tu), in various concentrations, were added to chemically defined plant tissue culture medium on which meloidogyne javanica was reared on excised tomato roots. concentrations as low as 3 ppm hu or 12 ppm tu inhibited nematode maturation by 70-90% 4 weeks after inoculation, and the coenocytes in the parasitized tissue were poorly developed. gall weight was also inhibited by 50% in cultures treated with 3 and 6 ppm hu. however, exposing juveniles of m. java ...198419295888
relationship between heterodera schachtii, meloidogyne hapla, and nacobbus aberrans on sugarbeet.heterodera schachtii, meloidogyne hapla, and nacobbus aberrans either alone, or in various combinations with each other, can, when inoculated at a concentration of 12 second-stage juveniles/ cm(3) of soil, cause a significant (p = 0.01) suppression of growth of sugarbeet (cv. tasco ah14) seedlings. m. hapla and h. schachtii decreased growth of sugarbeet more than n. aberrans over a 60-day period. the adverse effect of n. aberrans on the final population/initial population (pf/pi) ratio for eithe ...198419295890
nematicides and nonconventional soil amendments in the management of root-knot nematode on cotton.granular and liquid commercial humates, with micronutrients, and a microbial fermentation product were compared in several combinations with nematicides for their effects on cotton lint yield and root-knot nematode suppression. fumigant nematicides effectively reduced cotton root galling caused by root-knot nematodes, and cotton lint yields increased. organophosphates and carbamates were not effective. occasionally, cotton lint yields were increased or maintained with combination treatments o f ...198419295893
influence of meloidogyne chitwoodi and m. hapla on wheat growth.meloidogyne chitwoodi reduced the growth of winter wheat 'nugaines' directly in relation to nematode density in the greenhouse, the relationship between top dry weight and initial nematode density suggests a tolerance limit of nugaines wheat to m. chitwoodi of between 0.03 and 0.18 eggs/cm(3) of soil; the value for relative minimum plant top weight was 0.45 g and 0.75 g, respectively. growth of wheat in field microplots containing four population densities (0.003, 0.05, 0.75 and 9 eggs/cm(3) soi ...198419295895
meloidogyne microcephala n. sp. (meloidogynidae), a root-knot nematode from thailand.meloidogyne microcephala n. sp. is described and illustrated from specimens obtained from tobacco (nicotiana tabacum l.) in thailand. the female perineal pattern usually has a low dorsal arch, coarse striae, and a series of small cuticular flaps around the tail terminus. the stylet of the female is 14.4 mum long, with large, square to rectangular stylet knobs, the distinctive male head region is narrow, small, and truncate with a low, flattened head cap. the stylet length is 20.6 mum, and the kn ...198419295899
a new pathotype of root-knot nematode on grape rootstocks. 198419295902
histochemical localization and nematoxicity of terpenoid aldehydes in cotton.in healthy cotton, except for random occasional occurrence in cortical cells, terpenoid aldehydes (ta) are localized in the epidermis and, even there, are absent from the tip 2-4 cm of the root. since constitutive ta do not occur in the endodermis and stele of the root, they cannot be effective agents against the development of the sedentary stage of the root-knot nematode, meloidogyne incognita. within 4 days after inoculation with the root-knot nematode, infection-induced ta accumulated in the ...197919300641
nonfumigant nematicides for control of root-knot nematode to protect carrot root growth in organic soils.greenhouse tests were conducted to determine the effects of two kinds of meloidogyne hapla inoculum on the growth and quality of carrot roots, and the protection afforded in each case by nonfumigant nematicides in organic soils. for all treatments the percentage of carrots damaged was greater with larvae alone as inoculum than with larvae and eggs, indicating that most of the damage occurs early during formation of the taproot. fosthietan, aldicarb, and oxamyl at 4 and 6 kg ai/ha protected the r ...197919300652
partial characterization of the cuticle surface of meloidogyne javanica females.negative charges on the outer cuticular surface of meloidogyne javanica females were visualized with electron microscope labelling techniques. evidence is presented that the electronegative charge is not borne on neuraminic acid. ruthenium red staining indicated acid mucopolysaccharides on the outer surface. a surface coat, or glycocalyx, external to the outer cuticle membrane was demonstrated.197919300656
interrelations between meloidogyne javanica, rotylenchulus reniformis, and rhizobium sp. on vigna sinensis.the interactions of meloidogyne javanica, rotylenchulus reniformis, and rhizobium sp. on cowpea seedlings were investigated. upon simultaneous inoculation with the two nematode species, m. javanica invaded first but did not affect root invasion by r. reniformis. m. javanica populations increased less in competition with r. reniformis than when present alone. preinvasion by r. renilormis significantly suppressed the number of m. javanica in the roots. inoculation of m. javanica and/or r. reniform ...198019300672
effect of phenamiphos on heterodera schachtii and meloidogyne javanica.aqueous solutions of technical-grade phenamiphos [ethyl 3-methyl-4-(methylthio) phenyl (1-methylethyl) phosphoratnidale] were used in hatching chambers to test, under laboratory tory conditions, the effect of phenamiphos on the hatching and movement of meloiclogyne javanica and heterodera schachtii. hatch of m. javanica and h. schachtii eggs was depressed 70 and 88% by nematicide at 0.48 and 4.80 mug/ml, respectively. the infectivity of second-stage larvae of both species was affected by concent ...198019300678
inhibition of syncytia formation and root-knot nematode development on cultures of excised tomato roots.two different defined growth media were used to culture aseptically the root-knot nematode, meloidogyne incognita, on excised roots of tomato, lycopersicon esculentum cv 'marglobe.' one of these media, stw, was a formulation by skoog, tsui, and white and the other, ms, a formulation by murashige and skoog. from 1 through 4 weeks, inoculated tissues were fractured to observe root infection, giant-cell formation, and nematode development with the scanning electron microscope (sem). four weeks afte ...198019300696
interrelationship of meloidogyne hapla and ditylenchus dipsaci on resistant and susceptible alfalfa.simultaneous inoculations of alfalfa with meloidogyne hapla larvae and ditylenchus dipsaci at 16, 20, 24, and 28 c did not depress penetration of either nematode in 'nev syn xx' -a selection resistant to m. hapla and d. dipsaci, 'vernal 298' -a selection resistant to m. hapla and susceptible to d. dipsaci, 'lahontan' -a cultivar resistant to d. dipsaci and susceptible to m. hapla, and 'ranger' -a cultivar susceptible to both m. hapla and d, dipsaci. infection with d. dipsaci depressed growth of ...198019300705
effects of one and two applications of nematicides on nematode populations and soybean yields.yields of 'mcnair 800' soybeans, glycine max (l.) merr., were significantly increased with ethylene dibromide + chloropicrin, dbcp, phenamiphos, and aldicarb applied at-planting and with phenamiphos, aldicarb, and dbcp applied postplant to soil infested with meloidogyne incognita (kofoid and white) chitwood. yields of 'gasoy 17' were significantly increased with ethylene dibromide + chloropicrin, dbcp, phenamiphos, and aldicarb applied, preplant and with dbcp, carbofuran, phenamiphos, aldicarb, ...198019300706
description and sem observations of meloidogyne chitwoodi n. sp. (meloidogynidae), a root-knot nematode on potato in the pacific northwest.meloidogyne chitwoodi n. sp. is described and illustrated from potato (solanum tuberosum) originally collected from quincy, washington, usa. this new species resembles m. hapla, but its perineal pattern is basically round to oval with distinctive and broken, curled, or twisted striae around and above the anal area. the vulva is in a sunken area devoid of striae. vesicles or vesicle-like structures are present in the median bulb of females. the larva tail, being short and blunt with a hyaline tai ...198019300709
dynamics of concomitant populations of hoplolaimus columbus, scutellonema brachyurum, and meloidogyne incognita on cotton.cotton seedlings grown in a greenhouse and a growth chamber were inoculated with scutellonema brachyurum, hoplolaimus columbus, and meloidogyne incognita, singly and in all possible combinations, at two initial population (pi) levels (100 and 300/100 cm(3)). s. brachyurum alone was not pathogenic to cotton at these population levels. it fed primarily as an ectoparasite but matured and reproduced within the root when it penetrated. populations of s. brachyurum increased in the presence of h. colu ...198119300720
interaction between meloidogyne arenaria and glomus fascicuqlatus in grape.root zones of grape (fitis vinifera cv thompson seedless) cuttings were infested with chlamydospores of glomus fasciculatus or eggs of meloidogyne arenaria or both. growth of grapevines was greatest in mycorrhizal (g. fasciculatus) plants. mycorrhizal development and growth of mycorrhizal and nonmycorrhizal plants were reduced in the presence of m. arenaria. at low initial nematode inoculum (pi) levels (approx. 200 eggs/plant), the presence of mycorrhizae enhanced plant growth during 1 yr, but n ...198119300722
oogenesis and the chromosomes of the parthenogenic root-knot nematode meloidogyne incognita.220 populations of meloidogyne incognita and related forms from 46 countries reproduced by mitotic parthenogenesis (apomixis). determination of somatic chromosome numbers from oogonia and oocytes revealed the existence of a predominant, possibly triploid race a with 3n = 40 to 46 and a rare, diploid race b with 2n = 32 to 36 chromosomes. there is no correlation between cytological races and the four recognized host races of this species. the characteristic behavior of prophase i chromosomes of m ...198119300730
diacyl, alkylacyl, and alkenylacyl phospholipids of meloidogyne javanica females.the phospholipid composition and acyl, alkyl, and alkenyl group compositions of diacyl, alkylacyl, and alkenylacyl phosphoglycerides of m. javanica were investigated. phospholipid was comprised of 61.7% choline phosphoglyceride, 22.0% ethanolamine phosphoglyceride, and smaller quantities of six other lipids. phospholipid fatty acid was more unsaturated than neutral lipid fatty acid and contained 61.3% octadecenoic (18:1) acid. fatty acid at the 1-position of diacyl phospholipids was shorter and ...198119300731
comparative studies on root invasion, root galling, and fecundity of three meloidogyne spp. on a susceptible tobacco cultivar.root invasion, root galling, and fecundity of meloidogyne javanica, m. arenaria, and m. incognita on tobacco was compared in greenhouse and controlled environment experiments. significantly more m. javanica than m. arenaria or m. incognita larvae were found in tobacco roots at 2, 4, and 6 d after inoculation. eight days after inoculation there were significantly more m. arenaria and m. javanica than m. incognita larvae. ten days after inoculation no significant differences were found among the t ...198119300745
scanning electron microscope study of the root-knot nematode (meloidogyne incognita) on tomato root.this study examines the types of structural information that can be gained by utilizing the scanning electron microscope (sem) and a cryofracture technique to examine the host-parasite interaction. roots of tomato, lycopersicon esculentum cv. marglobe, were cultured aseptically and inoculated with the root-knot nematode, meloidogyne incognita. twenty-four hours to four weeks after inoculation, developing galls were removed from the cultures and processed for sem observation. the cryofracture tec ...198119300776
influence of volcanic ash on infectivity and reproduction of two species of meloidogyne.mount st. helens volcanic ash was incorporated into a loamy sand greenhouse soil mix to produce concentrations of 0, 0.5, 1.0, 2.0, 4.0, 8.0, 25, 50 and 100% ash. chemical and physical properties of the various mixtures were determined. three experiments were conducted in a greenhouse to determine if volcanic ash had any influence on root-knot nematode survival and infectivity. tomato, lycoperscion esculentum, seedlings cv. columbia, susceptible to meloidogyne hapla and m. chitwoodi were planted ...198119300784
three new species of heteroderoidea (nematoda) from the aleutian islands.three new species of heteroderoidea are described from adak and amchitka islands in the aleutian chain. second-stage juveniles of thecavermiculatus crassicrustata, n. sp., differ from those of t. gracililancea robbins by having longer stylets (40-45 mum vs 19-22 mum). the female of t. crassicrustata has a longer neck, a more posterior excretory pore, and lacks a posterior protuberance. meloidodera eurytyla, n. sp., differs from other meloidodera spp. in that second-stage juveniles have longer st ...198119300796
pathogenicity of the columbia root-knot nematode (meloidogyne chitwoodi) on wheat, corn, oat, and barley. 198119300804
control of meloidogyne javanica and m. arenaria on kenaf and roselle with genetic resistance and nematicides.kenaf (hibiscus cannabinus) and roselle (h. sabdarifla) were evaluated in nematicide-treated and untreated field soil naturally infested with either meloidogyne javanica or m. arenaria. root-knot indices indicated that the kenaf breeding line j-l-113 had moderate resistance to m. javanica and low resistance to m. arenaria. kenaf cv everglades 71 was highly susceptible to both m. javanica and m. arenaria, and roselle breeding line a59-56 was highly resistant. both nematode species reproduced on a ...197919305526
meloidogyne megatyla n. sp. a root-knot nematode from loblolly pine.meloidogyne megatyla n. sp. is described from pinus taeda in north carolina. stylet knobs are distinctively high in proportion to width, giving an especially massive appearance to the knobs of larvae and males. mean larval length is 416 mum and stylet length is 14.6 mum. the perineal pattern is composed of smooth striae, with a high arch, and is often somewhat rectangular. the relationship of m. megatyla to other meloidogyne species is unclear, although a comparison is made with meloidogyne inco ...197919305528
effect of oryzalin and 1,1-dimethylpiperidinium chloride on cotton and tomato roots infected with the root-knot nematode, meloidogyne incognita.oryzalin (3,5-dinitro-n4,n4-dipropyl-sulfanilamide) and bas 083 (l,l-dimethylpiperdinium chloride) reduced root-knot infection in tomato roots when respectively applied as a soil drench at 20 ppm and 10,000 ppm. oryzalin reduced knot counts with various intervals between treatment and inoculation. bas 083 reduced knot counts only when applied before inoculation. oryzalin was shown not to be a contact nematicide, and bas 083 was only a weak one. neither compound reduced penetration by infective l ...197919305533
the histopathological reactions of vigna sinensis to separate and concomitant parasitism by meloidogyne javanica and rotylenchulus reniformis.cellular alterations in cowpea roots and nodules induced by single and concomitant meloidogyne javanica and rotylenchulus reniformis were investigated. m. javanica induced giant cells inside the vascular bundles of roots and nodules, and syncytia in cortical tissue of the nodules. in contrast, r. reniformis stimulated hypertrophy of pericycle and endodermal cells of the roots and nodules. syncytia induced in the roots involved a sheet of pericycle cells and an endodermal cell. cortical ceils of ...197919305543
effects of nematicide placement on nematode populations and soybean yields.four methods of placement of dbcp (l,2-dibromo-3-chloropropane) and a single method of application of ethoprop (0-ethyl s,s-dipropyl phosphorodithioate) wexe compared in each of two areas for control of nematodes on soybeans. one area was a marlboro sand infested with hoplolaimus columbus. the other area was a fuquay loamy sand infested with meloidogne incognita. soybean yields were increased and numbers of h. columbus in the row 0-20 cm deep were decreased similarly by all methods of dbcp appli ...197919305549
quantitative aspects of the development of meloidogyne arenaria larvae in grapevine varieties and rootstocks.the development and productivity of parasitic stages of meloidogyne arenaria were quantitatively defined in 14 varieties or rootstocks of grapevine. mean development to maturity was related linearly to the number of degree-hours above 10 c temperature experienced from the time of penetration in all cultivars in which nematode adulthood was achieved. averaged across varieties, 13,142 heat units were required for development of the mean individual to maturity. the standard deviation of the develop ...197919305553
descriptions of meloidogyne camelliae n.sp. and m. querciana n.sp (nematoda:meloidogynidae), with sem and host-range observations.meloidogyne camelliae n.sp. on camellia (camellia japonica) from japan and m. querciana n.sp. on pin oak (quercus palustris) from virginia, usa, are described and illustrated. m. camelliae n.sp. is distingnishable from other species of the genus especially by its striking perineal pattern having heavy ropelike striae forming a squarish to rectangular outline with shoulders or projections, appearing sometimes ahnost starlike. m. querciana differs from other species by its characteristic perineal ...197919305554
infectivity of bacillus penetrans in plant-parasitic nematodes.larvae of meloidogyne spp. were readily infected with the endoparasite bacillus penetrans by exposure to an aqueous suspension of spores from infected root-knot nematode females, or by passage of larvae through a shallow layer (50 cm(3)) of spore-infested soil. infection severely reduced motility of second-stage larvae through soil. bacillus penetrans exhibited a distinct host specificity in that only 5 of 16 nematode species tested became infected with the population used. meloidogyne javanica, ...197719305569
penetration and development of meloidogyne hapla in resistant and susceptible alfalfa under differing temperatures.studies were conducted to examine under differing temperatures (12, 16, 20, 24, 28, and 32 c) the penetration anti development of meloidogyne hapla in resistant lines '298' and 'nev. syn xx', and susceptible 'lahontan' and 'ranger' hardy-type alfalfas. the results indicated that resistance to m. hapla was similar to that previously described for m. incognita in nonhardy alfalfa. although initial penetration in resistant seedlings was similar to that of susceptible seedlings, nematode larvae fail ...197719305571
the nature and role of metabolic leakage from root-knot nematode galls and infection by rhizoctonia solani.a severe root rot of tomato caused by meloidogyne incognita and rhizoctonia solani was associated with nutrient mobilization into gall tissue and root exudation. root decay did not develop when root exudates were continuously removed by leaching. when leachates were collected from m. incognita-infected and control roots and applied to roots of tomatoes inoculated with r. solani alone, roots receiving leachates from m. incognita-infected roots developed a severe rot while roots receiving leachate ...197719305577
effects of management practices on nematode and fungi populations and okra yield.okra was grown in field plots of tifton loamy sand naturally infested with the nematodes meloidogyne incognita and criconemoides ornalus and the pathogenic fungi fusarium oxysporum, f. solani, f. roseum, and pythium spp. plots were treated with various soil pesticides and left exposed or covered with biodegradable paper film mulch under trickle irrigation. soil was assayed for nematodes and fungi, and plant roots were examined for root-rot and insect damage. fewer nematodes and fungi generally w ...197719305580
terpenoid aldehydes in cotton roots susceptible and resistant to the root-knot nematode, meloidogyne incognita.we investigated the role of terpenoid aldehydes in the resistance of cotton (gossypium hirsutum) to the root-knot nematode (meloidogyne incognita). three-day-old, root-knot-resistant ('auburn 623') and -susceptible ('deltapine 16') seedlings were inoculated with m. incognita. comparable portions of inoculated and noninoculated roots were harvested 2, 4, 7, and 10 days later. terpenoid aldehydes were extracted, separated by thin-layer chromatography, eluted as their phloroglucinol derivatives, an ...197719305600
effects of population densities of meloidogyne hapla on growth and yield of tomato.growth and yield of 'veebrite' tomato were studied in 20-cm (i.d.) clay-tile microplots containing initially 260, 1,840, 6,120, or 27,950 meloidogyne hapla larvae/kg of soil. low nematode numbers stimulated, and the highest nematode population suppressed, vegetative plant growth. more tomatoes, with a higher total weight, were harvested from plants infested with 260 and 1,840 nematode larvae at planting than from those with initial densities of 6,120 and 27,950 larvae. at the two highest densiti ...197719305611
peroxidase and 6-phosphogluconate dehydrogenase in resistant and susceptible cotton infected by meloidogyne incognita.assays of specific activities and electrophoretic separations of multiple forms of 6-phosphogluconate dehydrogenase and peroxidase in cotton resistant and susceptible to meloidogyne incognita were conducted 6 days after inoculation. specific activities were greater in infected than in uninfected roots and also were greater in the resistant cultivar, 'clevewilt 6-3-5,' than in the susceptible culti.var, 'm8.' in uninfected roots, peroxidase activity was greater in clevewilt roots than in m8 roots ...197819305810
anatomical response of grain sorghum roots to meloidogyne incognita acrita.the cotton root-knot nematode, meloidogyne incognita acrita, reproduced on the roots of grain sorghum, causing syncytia in the cortex or stele of lateral roots. giant cells developed either singly with few nuclei or in groups with many nuclei. giant cells that developed in groups appeared the same as those which developed singly. the pericycle and endodermis were interrupted at the site of nematode invasion. large areas of these tissues were absent for one-third of the circumference of the stele ...197819305813
early stages of nematode-induced giant-cell formation in roots of impatiens balsamina.giant cells induced in roots of impatiens balsamina by meloidogyne javanica and meloidogyne incognita have been examined by light and electron microscopy. the first sign of giant-cell formation was division of cells surrounding a larva. cell plate alignment appeared to proceed normally, but cytokinesis was unsuccessful and binucleate cells formed subsequently. no wall breakdown was evident then or later. the number of nuclei appeared to increase by repeated mitosis without separation by cytokine ...197819305816
root-knot nematodes and the process of ageing in plants.infection of plants by root-knot nematodes is often accompanied by physiological changes characteristic of ageing. ultra-low tissue luminescence of infected plants indicated oxidation of cell-membrane lipids. cells with membranes subjected to oxidation lose some of their capacity for water retention. treating tomato and radish with lidocaine hydrochloride, an inhibitor of lipid oxidation, retarded above-ground symptoms of root-knot nematode infection and of ageing.197819305822
influence of chilling and freezing temperatures on infectivity of meloidogyne incognita and m. hapla.egg masses and second-stage larvae of meloidogyne incognita and m. hapla in soil were exposed to temperatures ranging from 20 to -8 c. temperature was lowered in 2-day intervals to 16, 12, 8, 4, 0, -4, and -8 c, and the nematodes remained at 4, 0, -4, or -8 c for 18, 14, 10, or 6 days, respectively. unhatched larvae of both species were more resistant to low temperatures than were embryonic stages. within the eggs of m. incognita, 7.5% of embryos and 48% of larval stages survived 14 days at 0 c, ...197819305834
reaction of citrus rootstocks to meloidogyne javanica.the response of citrus spp. and related rootstocks to a population of meloidogyne javanica was evaluated in a screenhouse experiment. palestine and rangpur lime, rough lemon, sour orange, sexton and thentriton tangelo, and volkamer lemon were not infected by m. javanica. galls and tip swellings were observed on the roots of poncirus triloliata and troyer citrange. there was no evidence of nematode development. symptoms induced by the nematode were stelar division, syncytia formation in the vascu ...197819305835
ontogeny of daucus carota infected with meloidogyne hapla.the ontogeny of carrots (daucus carota cv. 'spartan premium') grown under greenhouse conditions in pots of organic soil infected with meloidogyne hapla was influenced detrimentally as early as 4 days after seeding, as determined through analysis of plant surface area, dry weight, fresh weight, net assimilation rate, relative growth rate, and leaf-area ratio. only 58% of the diseased carrots were suitable for fresh market, compared with 97% of those grown in nematode-free soil. growth and develop ...197819305837
the effect of diflubenzuron on egg formation by the root-knot nematode. 197819305842
influence of soil fumigation on the fusarium-root-knot nematode disease complex of cotton in california.for control of the root-knot nematode, meloidogyne incognita, and the pathogenic wilt fungus, fusarium oxysporum, on cotton, soil fumigants were applied in the field at conventional and higher rates. conventional rates suppressed fusarium wilt but higher rates gave quicker early growth, better stands, less stand loss over the season, a lower percentage of plants infected with wilt, fewer plants with vascular discoloration, and fewer nematodes. the best treatment about doubled the yields of untre ...197819305846
meloidogyne incognita wartellei n. subsp. (meloidogynidae), a root-knot nematode on resistant soybeans in louisiana.meloidogyne incognita wartellei n. subsp, is described and illustrated from roots of soybean (glycine max l.) near washington, louisiana. it is rather limited in distribution in that state, being known at five locations comprising about 60,000 acres. it not only attacks commonly susceptihle soybeans but is a destructive pest on other commercial soybean varieties that are resistant to other forms of the m. incognita group in the area. this new subspecies is related most closely to m. i. incognita ...197819305855
temperature-based prediction of egg-mass production by meloidogyne incognita.a maturation-rate relationship for meloidogyne incognita on lycopersicon esculentum 'rutgers' was derived and used to estimate harvest dates for maximum egg hatch from laboratory cultures at ambient temperatures. daily maturation increments were totaled (nematode maturation total, nmt) and correlated with hatch from isolated white, yellow, and amber egg masses. hatch per mass fluctuated periodically from ca. 1.0 nmt, when egg masses were first visible, to 2.5 nmt by which time plants showed stre ...197819305857
evaluation of the protective and therapeutic properties of dbcp for control of root-knot nematode on tomato.twelve soil drenches over a period of 30 days with dbcp concentrations of 40 microg/ml did not completely prevent infection of tomato plants by root-knot nematode juveniles. repeated dbcp drenches of 40 microg/ml halted gall development during the drenches, but 10 days after drenching was discontinued galls were apparent. dbcp drenches at 200 microg/ml prevented tomato root development, and 40 microg/ml slowed it. ten microg/ml increased the height of root-knot-infected plants, but not their top ...197819305858
influence of low temperature on development of meloidogyne incognita and m. hapla eggs in egg masses.egg masses of meloidogyne incognita and m. hapla were placed in soil at 10, 12, 16, and 20 c. at regular intervals, eggs from samples of egg masses were released from the gelatinous matrices and their developmental stages recorded. the number of days necessary to complete each stage from gastrulation to hatch is given for each temperature. the minimal temperature threshold for the development of eggs was computed by linear regression to be 8.26 c for m. incognita and 6.74 c for m. hapla.197819305859
meloidogyne grahami n. sp. (meloidogynidae), a root-knot nematode on resistant tobacco in south carolina.meloidogyne grahami n. sp. is described and illustrated from specimens on tobacco (nicotiana tabacum) originally from florence, south carolina. considered for several years to be only a race of m. ineognita, this new species readily attacks nc-95 tobacco, a variety with resistance to the m. incognita group that is common in the major u.s. tobacco-producing areas. m. grahami n. sp. is related most closely to the three subspecies of the m. incognita group but differs from all of them, especially i ...197819305866
effects of aldicarb on fusarium wilt-root-knot nematode disease of cotton. 197819305871
pathogenicity of pratylenchus coffeae, scutellonema bradys, meloidogyne incognita, and rotylenchus reniformis on dioscorea rotundata.low populations (200 specimens per plant) of pratylenchus cofl'eae, scutellonema bradys, meloidogyne incognita, and rotylenchulus reniformis stimulated the development of tops, roots, and tubers of dioscorea rotundata "guinea" yam. we demonstrated experimentally that p. coffeae was responsible for the deterioration in quality of the yam tuber in puerto rico, a condition known as a dry rot of yam. initial populations of 600 p. coffeae, s. bradys, or m. incognita, and populations of 1,000 p. coffe ...197519308127
free amino acids in roots of infected cotton seedlings resistant and susceptible to meloidogyne incognita.quantities of free amino acids in segments of cotton roots resistant and susceptible to meloidogyne incognita were compared. following infection, the root-knot susceptible cultivar, m8, had greater percentage increases of certain individual free amino acids than the resistant cultivar, clevewilt, but the sum total of free amino acids was greatest in the resistant cultivar. more free amino acids were present in infected than in noninfected plants of both cultivars. the overall concn of glycine de ...197519308129
interaction of four soybean cultivars with subsoiling and a nematicide.yields of four soybean, glycine max, cultivars were increased with subsoiling under the row and application of the nematicide, dbcp i 1,2-dibromo-3-chloropropane) in tiflon sandy loam heavily infested with the root-knot nematode meloidogyne incognita. these cultivars represent four maturity groups: very early (v), "essex', early (vi), "davis': medium (vii), 'ransom'; and late (viii), ' hutton '. the average increase for the four cullivars was about the same for subsoiling or dbcp. when the treat ...197519308134
autoradiography of developing syncytia in cotton roots infected with meloidogyne incognita.cotton (gossypium hirsutum) seedlings, uniformly infected with meloidogyne incognita, were exposed for periods of 1-15 days to a nutrient solution containing tritium-labelled thymidine. syncytium formation began with the amalgamation of cells near the nematode head, and was followed by synchronized mitoses of the nuclei which had been incorporated into a single cell. syncytial nuclei synthesized dna in roots harvested 3, 6, 9, 12, and 15 days after inoculation. seedlings transferred from unlabel ...197519308135
temperature-induced phase transitions in nematode lipids and their influence on respiration.temperature-induced phase transitions estimated by electron spin resonance (esr) technique were ohscrved in the lipids of several nematode species. in both meloidogyne javanica and caenorhahditis elegans, there was a phase transition in their phospholipids from a liquid-crystalline state to a solid gel state at about 10 c. aphelenchus avenae also had a phase transition, but at about 20 c. with this species, the spin-label motion parameters indicated the transition was from the liquid-crystalline ...197519308140
the incorporation of photosynthates by meloidogyne javanica.the root-knot nematode, meloidogyne javanica, incorporated (1)c from its host after exposure of the plant to (1)co. this uptake was relatively slow and was not detected in nematodes exposed to a labelled plant for periods of 2 and 4 h, but was after 24 h. nematodes were grown in plants previously infected at weekly intervals to provide animals at various stages of growth. plants were harvested 24 h after exposure to the label and the rate of incorporation per unit area of nematode was measured. ...197519308142
effect of meloidogyne incognita on selected forest tree species.four or five growth stages of 14 forest tree species were tested for susceptibility to meloidogyne incognita at five inoculum levels. responses ranged from the highly susceptible 'china fir' to immune 'taiwania'. even highly susceptible species became increasingly tolerant at later growth stages, thus root-knot appears to be a greater problem in nurseries than in established forests. heavily suberized cells which restricted nematode development was the predominant host response in norway spruce, ...197519308146
body wall fine structure of the anterior region of meloidogyne incognita and heterodera glycines males.the body wall fine structure including the cuticle, hypodermis, and somatic muscles is similar in males of meloidogyne incognita and heterodera glycines. the cuticle can be regarded as basically three-layered in both species, but is much thicker in m. incognita than in h. glycines, and differences occur in surface markings. the chordal and interchordal hypodermis is syncytial. hypodermal tissue pervades the lip region, and lines the stomatal cavity and stylet shaft. various organelles and struct ...197519308152
effects of aldicarb on the behavior of heterodera schachtii and meloidogyne javanica.the toxic effects of sublethal concentrations of aldicarb were studied on eggs and second-stage larvae and males of heterodera schachtii and second-stage larvae only of meloidogyne javanica in a quartz sand substrate. aldicarb was more toxic to eggs of h. schachtii than to those of m. javanica. complete suppression of hatching occurred between 0.48 and 4.8 microg/ml aldicarb for h. schachtii whereas 100% inhibition of hatch of m. javanica occurred between 4.8 and 48.0 microg/ml. m. javanica hatc ...197519308160
meloidogyne incognita-induced changes in cell permeability of galled roots.electrolyte leakage of meloidogyne incognita-infected and healthy tomato roots was compared by conductivity measurements, and by compartmental analysis using rb. conductivity measurements suggested difference in electrolyte loss from healthy and galled roots. on a percentage basis, a greater rate of efflux occurred for healthy plants, but galled roots contain more electrolytes and may show a larger net loss. compartmental analysis indicated that: (i) the longer half-time for rb loss from vacuole ...197519308167
influence of meloidogyne incognita on the content of amino acids and nicotine in tobacco grown under gnotobiotic conditions.seedlings of meloidogyne incognita-resistant (n.c. 95) and -susceptible (mcnair 30) tobacco cultivars were grown aseptically for 55 days inside isolator chambers in autoclaved soil infested with 0 or 3,000 axenized eggs of m. incognita per 500 cc of soil. healthy and infected plants were compared. dry root weights of infected plants of resistant and susceptible cultivars were 16% and 84%, respectively, less than the controls. sixteen amino acids, including those precursors for nicotine, and nico ...197519308177
comparative fine structure of the stomatal region of males of meloidogyne incognita and heterodera glycines.the fine structure of the stylet, "guiding apparatus," anti protractor muscles of males of meloidogyne incognita and heterodera glycines is ehtcittated and compared. in both nematodes, the stylet cone is of greater electron density than the shaft. the cone is heterogeneous; shaft material extends into it for most of its length, whereas the shaft proper and knob regions are relatively homogeneous. the stylet lumnen is round throughout its length in h. glycines, but in m. incognita changes from ro ...197619308188
influence of pratylenchus vulnus and meloidogyne hapla on the growth of rootstocks of rose.pratylenchus vulnus is involved in a desease of rosa noisettiana 'manetti' rose rootstock characterized by darkening of roots, death of feeder roots, and stunting of entire plants. the disease is more severe when plants are grown in silt loam soil than when they are grown in sandy loam soil. the nematodes reproduce best in silt loam soil at 20 c. meloidogyne hapla did not affect the growh of manetti. rosa sp. 'dr. huey', manetti, and r. odorata rose rootstocks were found to be goos hosts for p. ...197619308189
self-interactions of meloidogyne hapla and of heterodera schachtii on beta vulgaris.double inoculations of sugar beet with larvae of meloidogyne hapla resulted in a higher galling incidence in only one treatment than did a single inoculation using the same number of larvae. double inoculations with larvae of heterodera schachtii, however, resulted in three- to five-fold more cysts in most cases than did single inoculations using the same number of larvae. in general, plants died more quickly after double inoculations than after single inoculations of the same total number of ei ...197619308195
life cycle, pathogenicity, histopathology, and host range of race 5 of the barley root-knot nematode.the optimum temperature for development of race 5 of meloidogyne naasi was 26 c. a life cycle was completed in 34 days. growth of sorghum was suppressed when inoculated with m. naasi. observations of m. naasi-infected sorghum roots demonstrated that roots were penetrated just behind the root cap; giant cells were generally initiated either in the procambial region or in very young phloem. when giant cells developed in the cortex, corresponding areas of the vascular system did not have an endoder ...197619308227
relationships of initial population densities of meloidogyne incognita and m. hapla to yield of tomato.microplots 80 x 100 cm, infested with varying initial population densities (p(i)) of meloidogyne incognita or m. hapla, were planted to tomato at two locations. experiments were conducted in a sandy loam soil at fletcher, n. c. (mountains) where the mean temperature for may to september is ca 20.7 c, and in a loamy saml at clayton, n. c. (coastal plain) where the mean temperature for may to septemher is ca 24.8 c. in these experimentally infested plots, m. incognita and m. hapla caused maximum y ...197619308228
control of the root-knot nematode, meloidogyne incognita, on mimosa (albizzia julibrissin) by chemical dips. 197219319246
the relationship between tobacco yield and time of infection with meloidogyne javanica.yield of tobacco was related to the amount of infection by meloidogyne javanica during the first month after transplanting. six nematicidal treatments significantly reduced infection during this period and subsequently increased yield. however, during the second month after transplanting, infection in plots treated with o-ethyl s,s-dipropyl phosphorodithioate (v-c 9-104) and a mixture of 80% chlorinated c hydrocarbons + 20% methyl isothioeyanate (dd + mencs) was not significantly different from ...197219319250
serological relationship of meloidogyne incognita and m. arenaria.eight to ten precipitin bands were formed in a double immunodiffusion system comparing antigens of adult females of meloidogyne incognita and m. arenaria. most of the precipitin bands, based on band position and coalescence, were common to both species. antiserum specific for m. incognita was prepared by cross absorption. two populations of m. incognita were serologically identical, whereas two populations of m. arenaria differed slightly with respect to one weak precipitin band.197219319255
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