Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| ultrasonographic features of hepatobiliary pathology in opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma. | infection with the liver fluke, opisthorchis viverrini (ov), is an important public health problem in northeast thailand where people have a habit of eating raw or inadequately cooked fish. opisthorchiasis is associated with a number of asymptomatic hepatobiliary abnormalities and cholangiocarcinoma (cca) that can be observed conveniently by ultrasonography. based on our accumulated data, characteristic features of ultrasonographic findings of hepatobiliary pathology in ov infection are reviewed ... | 2016 | 27956092 |
| suspected cases of cholangiocarcinoma seen in reference hospitals in lao people's democratic republic. | cholangiocarcinoma (cca) poses a significant public health problem in southeast asia, with chronic opisthorchis viverrini infection being the main risk factor. epidemiological data on cca is not available for lao people democratic republic (lao pdr), though there is a high prevalence of o. viverrini infection in the country. a hospital record-based retrospective study was carried out in six referral hospitals (four in the capital city, one in savannakhet, one in pakse) to assess the number of su ... | 2016 | 27965165 |
| identification and characterization of protein 14-3-3 in carcinogenic liver fluke opisthorchis viverrini. | protein 14-3-3s are abundant phospho-serine/threonine binding proteins, which are highly conserved among eukaryotes. members of this protein family mediate metabolism and signal transduction networks through binding to hundreds of other protein partners. protein 14-3-3s have been studied in other species of parasitic helminthes, but little is known about this protein in the carcinogenic liver fluke opisthorchis viverrini. in this study, we identified and characterized protein 14-3-3s of o. viver ... | 2016 | 27989833 |
| nitrative and oxidative dna damage in infection-related carcinogenesis in relation to cancer stem cells. | infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells, and result in the formation of oxidative and nitrative dna lesions, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in inflammation-mediated carcinogenesis. it has bee ... | 2016 | 28050219 |
| [control of opisthorchis viverrini infection for cholangiocarcinoma prevention]. | the international agency for research on cancer (iarc) has classified two liver flukes as carcinogenic to humans (group 1): opisthorchis viverrini in 1994 and clonorchis sinensis in 2009. this review is focused on o. viverrini, the most studied of these two trematodes, which infects nearly 10 million people in southeast asia. the life cycle involves two intermediate hosts living in fresh water: a snail of the genus bithynia and a ciprinid fish. the definitive hosts (human, cat, dog) become infec ... | 2017 | 28105582 |
| application of environmental dna analysis for the detection of opisthorchis viverrini dna in water samples. | opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in southeast asian countries. the trematode, opisthorchis viverrini, is the causative agent of the disease. accurate and rapid monitoring of o. viverrini is crucial for disease prevention and containment. therefore, in this study we sought to develop a novel species-specific real-time pcr assay for detecting o. viverrini using environmental dna (edna). the diagnostic sensitivity ... | 2017 | 28108370 |
| targeting hexokinase ii as a possible therapy for cholangiocarcinoma. | overexpression of hexokinase 2 (hkii) has been demonstrated in various cancers. a number of in vitro and in vivo studies in several cancers show the significance of hkii in many cellular processes including proliferation, metastasis and apoptosis. however, the role of hkii in opisthorchis viverrini (ov) associated cholangiocarcinoma (cca) is still unknown. in the present study, the expression and roles of hkii were determined in ov associated cca. the expression of hkii was investigated in 82 pa ... | 2017 | 28131825 |
| coinfection with helicobacter pylori and opisthorchis viverrini enhances the severity of hepatobiliary abnormalities in hamsters. | persistent infection with opisthorchis viverrini causes hepatobiliary abnormalities, predisposing infected individuals to cholangiocarcinoma (cca). in addition, helicobacter pylori is highly prevalent in most countries and is a possible risk factor for cca; however, its role in enhancing hepatobiliary abnormality is unclear. here, we investigated the effects of coinfection with h. pylori and o. viverrini on hepatobiliary abnormality. hamsters were divided into four groups: (i) normal, (ii) h. py ... | 2017 | 28138021 |
| nitrative and oxidative dna damage in infection-related carcinogenesis in relation to cancer stem cells. | infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells, and result in the formation of oxidative and nitrative dna lesions, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in inflammation-mediated carcinogenesis. it has bee ... | 2017 | 28163802 |
| efficacy and safety of praziquantel against light infections of opisthorchis viverrini: a randomized parallel single-blind dose-ranging trial. | 2017 | 28174906 | |
| social network analysis of food sharing among households in opisthorchiasis endemic villages of lawa lake, thailand. | consumption of raw fish is a well-documented risk factor for opisthorchis viverrini infection. sharing of food, especially raw fish recipes may influence the spread of disease through a community. using social network analysis of an ego network, we investigated food sharing among households in an opisthorchis-endemic area. network centrality properties were used to explain the differences in o. viverrini transmission and control between villages with a low and high prevalence of infection. infor ... | 2017 | 28188767 |
| morphological and molecular identification of the liver fluke opisthorchis viverrini in the first intermediate host bithynia snails and its prevalence in kampong cham province, cambodia. | 2017 | 28189767 | |
| elevated prevalence of helicobacter species and virulence factors in opisthorchiasis and associated hepatobiliary disease. | recent reports suggest that opisthorchis viverrini serves as a reservoir of helicobacter and implicate helicobacter in pathogenesis of opisthorchiasis-associated cholangiocarcinoma (cca). here, 553 age-sex matched cases and controls, 293 and 260 positive and negative for liver fluke o. viverrini eggs, of residents in northeastern thailand were investigated for associations among infection with liver fluke, helicobacter and hepatobiliary fibrosis. the prevalence of h. pylori infection was higher ... | 2017 | 28198451 |
| role of socio-cultural and economic factors in cyprinid fish distribution networks and consumption in lawa lake region, northeast thailand: novel perspectives on opisthorchis viverrini transmission dynamics. | opisthorchis viverrini (ov) is a fish-borne parasite endemic in parts of lao pdr, cambodia, southern vietnam and northeast thailand (isaan) where an estimated 10 million people are infected. human ov infection, associated with hepatobiliary complications, including cholangiocarcinoma (cca), occurs when infected fish are consumed raw or undercooked, a longstanding cultural tradition in the region. this mixed- methods descriptive study was carried out in isaan villages around lawa lake, khon kaen ... | 2017 | 28216369 |
| differential protein expression marks the transition from infection with opisthorchis viverrini to cholangiocarcinoma. | parts of southeast asia have the highest incidence of intrahepatic cholangiocarcinoma (cca) in the world due to infection by the liver fluke opisthorchis viverrini (ov). ov-associated cca is the culmination of chronic ov-infection, with the persistent production of the growth factors and cytokines associated with persistent inflammation, which can endure for years in ov-infected individuals prior to transitioning to cca. isobaric labelling and tandem mass spectrometry of liver tissue from a hams ... | 2017 | 28232516 |
| effects of albendazole, artesunate, praziquantel and miltefosine, on opisthorchis viverrini cercariae and mature metacercariae. | to explore larvicidal effects of anthelmintic drugs on opisthorchis viverrini (o. viverrini) for alternative approach to interrupting its cycle for developing a field-based control program. | 2017 | 28237476 |
| experimental and modelling investigations of opisthorchis viverrini miracidia transmission over time and across temperatures: implications for control. | transmissibility is a significant factor in parasite fitness. the rate and magnitude of parasite transmission affect prevalence and infection intensity in individual hosts and are influenced by environmental factors. in this context, the objectives of this study were: (i) to experimentally assess opisthorchis viverrini miracidia survival and infectivity over time and across temperatures; and (ii) to combine these experimental results with environmental data to build a key component of a transmis ... | 2017 | 28237890 |
| preliminary genetic evidence of two different populations of opisthorchis viverrini in lao pdr. | opisthorchis viverrini is a major public health concern in southeast asia. various reports have suggested that this parasite may represent a species complex, with genetic structure in the region perhaps being dictated by geographical factors and different species of intermediate hosts. we used four microsatellite loci to analyze o. viverrini adult worms originating from six species of cyprinid fish in thailand and lao pdr. two distinct o. viverrini populations were observed. in ban phai, thailan ... | 2017 | 28238124 |
| opisthorchis viverrini infection in the snail and fish intermediate hosts in central vietnam. | opisthorchis viverrini, a carcinogenic fish borne fluke, requires freshwater snails and fish as intermediate hosts. opisthorchiasis is endemic in parts of southeast asia, including central and south vietnam. in this region the transmission by intermediate hosts has received little attention. therefore, freshwater snails and wild fish from bau my tho, an opisthorchiasis endemic area in binh dinh province were collected for examination of o. viverrini cercariae and metacercariae, respectively. a t ... | 2017 | 28242064 |
| clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin β4-fak/src signaling pathway. | cholangiocarcinoma (cca) is a slow-growing but highly metastatic cancer. its metastatic potential largely explains its high mortality rate. a recognized risk factor for cca development is infection with the liver flukes opisthorchis viverrini and clonorchis sinensis. we previously reported that the excretory-secretory products (esps) of c. sinensis promoted the three-dimensional aggregation and invasion of cca cells. in the present study, a quantitative real-time pcr array of extracellular matri ... | 2017 | 28286026 |
| epidemiological investigation of parasitic infection of schoolchildren from six elementary schools in sakon nakhon province, thailand. | we conducted an epidemiological study of intestinal parasitic infection in 572 schoolchildren aged 4 to 12 years old from six elementary schools in sakon nakhon province, thailand from june 2013 to august 2014. we collected fecal, blood, and urine samples to investigate parasitic infection and conducted a questionnaire survey. soil samples were examined for egg contamination. fecal examination, using the formalin-ether sedimentation method, revealed that 39% of schoolchildren were infected with ... | 2017 | 28289269 |
| recurrent emergence of structural variants of ltr retrotransposon csrn1 evolving novel expression strategy and their selective expansion in a carcinogenic liver fluke, clonorchis sinensis. | autonomous retrotransposons, in which replication and transcription are coupled, encode the essential gag and pol genes as a fusion or separate overlapping form(s) that are expressed in single transcripts regulated by a common upstream promoter. the element-specific expression strategies have driven development of relevant translational recoding mechanisms including ribosomal frameshifting to satisfy the protein stoichiometry critical for the assembly of infectious virus-like particles. retrotra ... | 2017 | 28322871 |
| delineating distinct heme-scavenging and binding functions of domains in mf6p/hdm proteins from parasitic flatworms. | mf6p/fhhdm-1 is a small protein secreted by the parasitic flatworm (trematode) fasciola hepatica and belonging to a broad family of heme-binding proteins (mf6p/hdms). mf6p/hdms are of interest for understanding heme homeostasis in trematodes and also as potential targets for the development of new flukicides. moreover, interest in these molecules has also increased due to their immunomodulatory properties. here we have extended our previous findings on the mechanism of mf6p/hdm-heme interactions ... | 2017 | 28348084 |
| efficacy of moxidectin versus ivermectin against strongyloides stercoralis infections: a randomized controlled non-inferiority trial. | infections with strongyloides stercoralis are of considerable public health relevance. moxidectin, a well-established drug in veterinary medicine under consideration for regulatory submission for the treatment of onchocerciasis, might serve as alternative to the most widely used ivermectin. | 2017 | 28369530 |
| fish sharing as a risk factor for opisthorchis viverrini infection: evidence from two villages in north-eastern thailand. | foodborne trematodiasis (fbt) is a significant global health problem, with the liver flukes opisthorchis viverrini, o. felineus, and clonorchis sinensis contributing to half of the global burden of fbt. north-eastern thailand where o. viverrini is endemic and un-cooked fish dishes remain an integral part of the food culture has the highest reported incidence of opisthorchiasis, including associated cholangiocarcinoma. both food sharing and eating practices are potentially important factors in ft ... | 2017 | 28372560 |
| development of a potent wound healing agent based on the liver fluke granulin structural fold. | granulins are a family of protein growth factors that are involved in cell proliferation. an orthologue of granulin from the human parasitic liver fluke opisthorchis viverrini, known as ov-grn-1, induces angiogenesis and accelerates wound repair. recombinant ov-grn-1 production is complex and poses an obstacle for clinical development. to identify the bioactive region(s) of ov-grn-1, four truncated n-terminal analogues were synthesized and characterized structurally using nmr spectroscopy. pepti ... | 2017 | 28425707 |
| the role of domestic dogs in the transmission of zoonotic helminthes in a rural area of mekong river basin. | dogs have been bred since ancient times for companionship, hunting, protection, shepherding and other human activities. some canine helminth parasites can cause significant clinical diseases in humans as opisthorchis viverrini causing cholangiocarcinoma in southeast asian countries. in this study, socio-cultural questionnaire, canine parasitological analysis, necropsy, parasite molecular confirmation and dog roaming data were evaluated in savannakhet, lao-pdr, a typical mekong basin area. dog ow ... | 2017 | 28426425 |
| first report and molecular identification of opisthorchis viverrini infection in human communities from lower myanmar. | opisthorchis viverrini is endemic in the south east asian region, especially in cambodia, lao people's democratic republic, vietnam and thailand, but there have been no previous records from myanmar. during stool surveys of rural populations in three regions of lower myanmar, opisthorchis-like eggs were found in 34 out of 364 (9.3%) participants by stool microscopy after using the modified formalin-ether concentration technique. dna was extracted from these positive stool samples and a portion o ... | 2017 | 28472153 |
| codon usage patterns of tyrosinase genes in clonorchis sinensis. | codon usage bias (cub) is a unique property of genomes and has contributed to the better understanding of the molecular features and the evolution processes of particular gene. in this study, genetic indices associated with cub, including relative synonymous codon usage and effective numbers of codons, as well as the nucleotide composition, were investigated in the clonorchis sinensis tyrosinase genes and their platyhelminth orthologs, which play an important role in the eggshell formation. the ... | 2017 | 28506040 |
| present situation of opisthorchiasis in vientiane capital, lao peoples' democratic republic. | opisthorchiasis is the commonest liver disease caused by opisthorchis viverrini in thailand, lao people's democratic republic (pdr) and cambodia, which will be a major cause of future human cholangiocarcinoma in these regions. in this short review, we describe the epidemic aspects of this parasite infection and the outline of results of our recent surveys of the fluke in humans and its intermediate hosts in vientiane capital, where information on the opisthorchiasis epidemic is insufficient. we ... | 2017 | 28552889 |
| lineage-specific expansion and loss of tyrosinase genes across platyhelminths and their induction profiles in the carcinogenic oriental liver fluke, clonorchis sinensis. | tyrosinase provides an essential activity during egg production in diverse platyhelminths by mediating sclerotization of eggshells. in this study, we investigated the genomic and evolutionary features of tyrosinases in parasitic platyhelminths whose genomic information is available. a pair of paralogous tyrosinases was detected in most trematodes, whereas they were lost in cyclophyllidean cestodes. a pseudophyllidean cestode displaying egg biology similar to that of trematodes possessed an ortho ... | 2017 | 28583212 |
| nimotuzumab inhibits cholangiocarcinoma cell metastasis via suppression of the epithelial-mesenchymal transition process. | changes in epidermal growth factor receptor (egfr) are commonly found in cancer progression, signaling a poor outcome in patients. in the present study, we aimed to investigate whether nimotuzumab could be of benefit for cholangiocarcinoma (cca) treatment. | 2017 | 28668850 |
| simplified techniques for killing the carcinogenic, opisthorchis viverrini metacercariae in cyprinid fish | consumption of fluke-free fish is the most important factor in controlling opisthorchis viverrini (ov) infection in endemic areas such as northeast thailand and thereby reducing the risk of cholangiocarcinoma. cooking fish is the best way to avoid infection; however, the cultural practice of eating raw or fermented fish is difficult to change. we investigated the food preparation process, using freezing, heating and fermentation to kill ov metacercariae in fish. uncooked cyprinid fish infected w ... | 2017 | 28669159 |
| protein kinases as targets for opisthorchis viverrini- associated cholangiocarcinoma therapy. | protein kinases are enzymes that catalyze the transfer of phosphate from atp to the serine/threonine or tyrosine residues of target molecules. these are key important mediators in a signaling cascade involved in several biological processes. dysregulation of their activity has been found in various tumors. from our increased understanding of kinase structure and activation mechanisms there emerged new strategies for targeting kinase in cancer treatment. nowadays, kinase specific inhibitors are d ... | 2017 | 28699535 |
| subsets of inflammatory cytokine gene polymorphisms are associated with risk of carcinogenic liver fluke opisthorchis viverrini-associated advanced periductal fibrosis and cholangiocarcinoma. | opisthorchis viverrini infection induces chronic inflammation, and a minor proportion of infected individuals develop advanced periductal fibrosis (apf) and cholangiocarcinoma (cca). inflammatory cytokines and/or their gene polymorphisms may link to these biliary pathologies. we therefore investigated associations among cytokine gene polymorphisms and cytokine production in 510 thai cases infected with o. viverrini who presented with apf+ or apf-, as established by abdominal ultrasonography as w ... | 2017 | 28719954 |
| ultrastructural characters of the spermatozoon of the liver fluke opisthorchis viverrini (poirier, 1886) (opisthorchiidae). | the present study records the ultrastructural organization of the mature spermatozoon of opisthorchis viverrini by means of transmission electron microscopy. the spermatozoon of o. viverrini is a filiform cell, tapered at both extremities. it exhibits the characteristics of type iv spermatozoon of digeneans, namely with two axonemes of the 9+'1' trepaxonematan pattern, external ornamentation of the plasma membrane associated with cortical microtubules that are in the posterior part of the anteri ... | 2017 | 28725936 |
| why does infection with some helminths cause cancer? | infections with opisthorchis viverrini, clonorchis sinensis, and schistosoma haematobium are classified as group 1 biological carcinogens: definitive causes of cancer. these worms are metazoan eukaryotes, unlike the other group 1 carcinogens including human papilloma virus, hepatitis c virus, and helicobacter pylori. by contrast, infections with phylogenetic relatives of these helminths, also trematodes of the phylum platyhelminthes and major human pathogens, are not carcinogenic. these inconsis ... | 2015 | 28741473 |
| prospects and challenges towards sustainable liver fluke control. | the liver fluke opisthorchis viverrini (ov) is endemic in southeast asia where more than 10 million people are estimated to be infected. the infection is associated with several hepatobiliary diseases, including cholangiocarcinoma (cca). northeast thailand is a hotspot for ov transmission, and, despite extensive public health prevention campaigns led by the government, the prevalence of ov infection is still high. high infection rates result from cultural and ecological complexities where wet-ri ... | 2017 | 28754415 |
| comparison of novel and standard diagnostic tools for the detection of schistosoma mekongi infection in lao people's democratic republic and cambodia. | given the restricted distribution of schistosoma mekongi in one province in lao people's democratic republic (lao pdr) and two provinces in cambodia, together with progress of the national control programmes aimed at reducing morbidity and infection prevalence, the elimination of schistosomiasis mekongi seems feasible. however, sensitive diagnostic tools will be required to determine whether elimination has been achieved. we compared several standard and novel diagnostic tools in s. mekongi-ende ... | 2017 | 28793922 |
| the battle of worldviews: a case study of liver fluke infection in khon kaen, thailand. | control efforts to reduce infection from the parasitic flatworm opisthorchis viverrini have progressed through understanding the epidemiology of opisthorchis viverrini, antiparasitic drug developments, technological innovations, health education promoting cooking of fish, and improved hygienic defecation. yet the problem persists. the case study method was used to examine the fundamental cause of the liver fluke infection problem. evidence shows that the liver fluke-infected population does not ... | 2017 | 28795594 |
| opisthorchiasis and cholangiocarcinoma in southeast asia: an unresolved problem. | the prevalence of cholangiocarcinoma (cca) in southeast asia is much higher than other areas of the world. eating raw, fermented, or undercooked cyprinid fish, infected with the liver fluke, opisthorchis viverrini sensu lato (sl), results in chronic biliary inflammation, periductal fibrosis, and increased cancer risk. there may be associated glomerulonephritis. the process of infection is difficult to disrupt because eating practices have proven extremely difficult to change, and the life cycle ... | 2017 | 28848361 |