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degradation of oxo-biodegradable plastic by pleurotus ostreatus.growing concerns regarding the impact of the accumulation of plastic waste over several decades on the environmental have led to the development of biodegradable plastic. these plastics can be degraded by microorganisms and absorbed by the environment and are therefore gaining public support as a possible alternative to petroleum-derived plastics. among the developed biodegradable plastics, oxo-biodegradable polymers have been used to produce plastic bags. exposure of this waste plastic to ultra ...201323967057
comparative effects of oyster mushrooms on lipid profile, liver and kidney function in hypercholesterolemic rats.comparative effects of oyster mushrooms on plasma and fecal lipid profiles and on liver and kidney function were evaluated in hyper and normocholesterolemic rats. feeding of hypercholesterolemic rats a 5% powder of oyster mushrooms (pleurotus ostreatus, p. sajor-caju and p. florida) reduced the plasma total cholesterol level by 37%, 21% and 16%, respectively and reduced the triglyceride level by 45%, 24% and 14%, respectively. ldl/hdl ratio decreased by 64%, 45% and 41% for p. sajor-caju, p. ost ...200923983505
isolation of genes specifically expressed in different developmental stages of pleurotus ostreatus using macroarray analysis.the oyster mushroom (pleurotus ostreatus) is one of the most important edible mushrooms worldwide. the mechanism of p. ostreatus fruiting body development has been of interest both for the basic understanding of the phenotypic change of the mycelium-fruiting body and to improve breeding of the mushrooms. based on our previous publication of p. ostreatus expressed sequence tag database, 1,528 unigene clones were used in macroarray analysis of mycelium, fruiting body and basidiospore developmental ...200923983539
vegetative growth and phylogenetic relationship of commercially cultivated strains of pleurotus eryngii based on its sequence and rapd.pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. this study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. optimal mycelial growth was observed at 30℃ and minimum mycelial growth observed at 10℃. this mushroom tolerates a broad ph range for mycelial growth, with most favorable growth observed at ph 6. results also indicated that glucose peptone ...200923983545
cryopreservation at -20 and -70 °c of pleurotus ostreatus on grains.alternative substrates for cryopreservation at -20 °c have been little explored for basidiomycetes and could bring new possibilities of lower cost cryopreservation. nevertheless, freezing temperatures between -15 and -60 °c are very challenging because they frequently result in cryoinjuries. the objective of this study was to evaluate substrates associated to cryoprotective agents for pleurotus ostreatus cryopreservation at -20 or -70 °c in order to develop alternative techniques for basidiomyce ...201223997343
isolation of bacteria associated with the king oyster mushroom, pleurotus eryngii.eight distinct bacteria were isolated form diseased mycelia of the edible mushroom, pleurotus eryngii. 16s rdna sequence analysis showed that the isolates belonged to a variety of bacterial genera including bacillus (lbs5), enterobacter (lbs1), sphingomonas (lbs8 and lbs10), staphylococcus (lbs3, lbs4 and lbs9) and moraxella (lbs6). among them, 4 bacterial isolates including lbs1, lbs4, lbs5, and lbs9 evidenced growth inhibitory activity on the mushroom mycelia. the inhibitory activity on the gr ...200823997601
effect of casing layer on growth promotion of the edible mushroom pleurotus ostreatus.various bacteria were isolated from the casing layer soil of the culture bed of p. ostreatus and their role in fruiting body induction of the edible mushroom, p. ostreatus, was investigated. analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. bordetella was predominant in the bulk soil whereas flavobacterium was predominant after sterilization of the casing ...200823997606
fruitbody development of pleurotus ostreatus via bottle cultivation using recycled substrate.this study was carried out to determine the possibility of bottle cultivation utilizing recycled oyster mushroom culture waste as a cultivating substrate for p. ostreatus. total nitrogen percentage was 0.76%, 1.13%, 1.16%, 1.36%, and 1.38% in the 1-, 2-, 3-, 4-, and 5-time mixed substrate, respectively; 0.95%, 1.04%, 1.34%, 1.36%, and 1.25% in the 1-, 2-, 3-, 4-, and 5-time postharvest substrate, respectively; and 0.72% and 0.68% in the 2- and 3-time nonadditive substrate, respectively. weight o ...200823997617
retrotransposon microsatellite amplified polymorphism strain fingerprinting markers applicable to various mushroom species.the retrotransposon mary1 is a gypsy family retroelement, which is detected ubiquitously within the fungal taxonomic groups in which mushrooms are included. to utilize mary1 as a molecular marker for the dna fingerprinting of mushrooms, oligonucleotides mary1-ltr-l and mary1-ltr-r were designed on the basis of highly conserved regions from the multiple sequence alignment of 30 mary1 sequences retrieved from a nucleotide sequence database. in accordance with retrotransposon microsatellite amplifi ...200823997618
nutritional analysis of cultivated mushrooms in bangladesh - pleurotus ostreatus, pleurotus sajor-caju, pleurotus florida and calocybe indica.mushroom cultivation has been started recently in bangladesh. awareness of the nutritional and medicinal importance of mushrooms is not extensive. in this study, the nutritional values of dietary mushrooms- pleurotus ostreatus, pleurotus sajorcaju, pleurotus florida and calocybe indica that are very popular among the cultivated mushrooms in bangladesh have been determined. these mushrooms were rich in proteins (20~25%) and fibers (13~24% in dry samples) and contained a lower amount of lipid (4 t ...200823997631
in vitro antagonistic characteristics of bacilli isolates against trichoderma spp. and three species of mushrooms.twenty isolates of bacillus species obtained from livestock manure composts and cotton-waste composts were tested for their antagonistic effects in vitro against three green mold pathogens of mushrooms (trichoderma harzianum, t. koningii, and t. viridescens). however, there exists a possibility bacillus species may have antagonistic effects against mushrooms themselves, and thus the same 20 isolates were tested in vitro against three species of mushrooms (flammulina velutipes, lentinus edodes, a ...200823997638
pleurotus ostreatus biofilms exhibit higher tolerance to toxicants than free-floating counterparts.the mbec(tm)-high throughput assay based on the calgary biofilm device was used to produce and to characterize pleurotus ostreatus biofilms. hydroxyapatite coating of pegs was required to enable biofilm attachment; biofilm amounts and homogeneity of distribution were markedly improved upon removal of non-sessile biomass after 48 h from inoculation. scanning electron microscopy showed surface-associated and multi-layered growth stabilized by the presence of an extracellular matrix (ecm). biofilms ...201323998200
liposome-mediated mycelial transformation of filamentous fungi.liposome-mediated transformation is common for cells with no cell wall, but has very limited usage in cells with walls, such as bacteria, fungi, and plants. in this study, we developed a procedure to introduce dna into mycelium of filamentous fungi, rhizopus nigricans lh 21 and pleurotus ostreatus td 300, by liposome-mediation but with no protoplast preparation. the dna was transformed into r. nigricans via plasmid pegfp-c1 and into p. ostreatus via 7.2 kb linear dna. the mycelia were ground in ...201324012298
valorization of solid olive mill wastes by cultivation of a local strain of edible mushrooms.olive oil industry generates huge quantities of solid olive mill wastes (somw), causing environmental damage. cultivation of edible mushrooms, such as pleurotus ostreatus is a valuable approach for somw valorization. a local strain mycelium (tizi-ouzou, algeria) of p. ostreatus (lpo) was isolated from castor oil plants. oyster mushroom spawn, produced on barley grains, was used to inoculate wet somw, steamed in a traditional steamer during 45 min. the mycelium growth rate on somw was first estim ...201324018198
solid-state fermentation of rapeseed meal with the white-rot fungi trametes versicolor and pleurotus ostreatus.rapeseed meal is valuable high-protein forage, but its nutritional value is significantly reduced by the presence of a number of antinutrients, including phenolic compounds. solid-state fermentation with white-rot fungi was used to decrease the sinapic acid concentration of rapeseed meal. after 7 days of growth of trametes versicolor and pleurotus ostreatus, the sinapic acid content of rapeseed meal was reduced by 59.9 and 74.5 %, respectively. at the end of the experiment, sinapic acid concentr ...201324022781
screening for ligninolytic enzymes from autochthonous fungi and applications for decolorization of remazole marine blue.this study presents new and alternative fungal strains for the production of ligninolytic enzymes which have great potential to use in industrial and biotechnological processes. thirty autochthonous fungal strains were harvested from bornova-izmir in turkiye. in the fresh fruitbody extracts laccase, manganese peroxidase and lignin peroxidase activities, which are the principal enzymes responsible for ligninocellulose degradation by basidiomycetes, were screened. spores of some of the basidiomyce ...200924031371
applicability of the use of waste from different banana cultivars for the cultivation of the oyster mushroom.the objective of this research was to evaluate the oyster mushroom pleurotus ostreatus- (jacq.: fr.) kumm. cultivation in substrates based on different combinations of wastes (leaf, pseudo-stem and pseudo-stem + leaf) and banana cultivars - musa spp. (thap maeo, prata anãpelipita and caipira) during 49 days. organic matter loss in the substrate by action of the fungus was also evaluated during that period. it was verified that the pseudo-stem waste provided the best averages of biological effici ...201224031895
lignocellulolytic enzyme production of pleurotus ostreatus growth in agroindustrial wastes.the mushroom pleurotus ostreatus has nutritional and medicinal characteristics that depend on the growth substrate. in nature, this fungus grows on dead wood, but it can be artificially cultivated on agricultural wastes (coffee husks, eucalyptus sawdust, corncobs and sugar cane bagasse). the degradation of agricultural wastes involves some enzyme complexes made up of oxidative (laccase, manganese peroxidase and lignin peroxidase) and hydrolytic enzymes (cellulases, xylanases and tanases). unders ...201224031982
two new species of trichoderma associated with green mold of oyster mushroom cultivation in korea.this paper describes and illustrates two new species, trichoderma pleurotum and t. pleuroticola, associated with green mold disease of oyster mushroom in korea.200624039481
non-additive transcriptional profiles underlie dikaryotic superiority in pleurotus ostreatus laccase activity.the basidiomycete pleurotus ostreatus is an efficient producer of laccases, a group of enzymes appreciated for their use in multiple industrial processes. the aim of this study was to reveal the molecular basis of the superiority of laccase production by dikaryotic strains compared to their parental monokaryons.201324039902
the effect of oyster mushroom β-1.3/1.6-d-glucan and oxytetracycline antibiotic on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in common carp (cyprinus carpio l.).the aim of the study was to evaluate the effect of micronized β-1.3/1.6-d-glucan (bg) derived from the oyster mushroom pleurotus ostreatus hiratake and tetracycline antibiotic oxytetracycline (otc) on biometrical, haematological, biochemical, and immunological indices, and histopathological changes in tissues of one- to two-year-old common carp (cyprinus carpio l.). the fish tested were divided into five experimental groups and one control. carp in the control group were fed commercial carp feed ...201324041844
changes of enzyme activities and compositions of abnormal fruiting bodies grown under artificial environmental conditions in pleurotus ostreatus.this study investigated the biochemical changes of abnormal fruiting bodies grown under artificial environmental conditions in p. ostreatus. abnormal mushroom growth during cultivation damages the production of good quality mushroom. this study showed that different environmental conditions produced morphological changes in the fruiting bodies of p. ostreatus. the fruiting bodies with morphological changes were collected and examined for differences in biochemical properties, enzyme activities, ...200524049471
changes in chemical composition and digestibility of three maize stover components digested by white-rot fungi.maize stover (total stem and leaves) is not considered a ruminant feed of high nutritive value. therefore, an improvement in its digestibility may increase the viability of total forage maize production systems in marginal growth regions. the objective of this study was to describe the changes in chemical composition during the storage of contrasting components of maize stover (leaf, upper stem and lower stem) treated with either of two lignin degrading white-rot fungi (wrf; pleurotus ostreatus, ...201424112093
inactivation of a pleurotus ostreatus versatile peroxidase-encoding gene (mnp2) results in reduced lignin degradation.lignin biodegradation by white-rot fungi is pivotal to the earth's carbon cycle. manganese peroxidases (mnps), the most common extracellular ligninolytic peroxidases produced by white-rot fungi, are considered key in ligninolysis. pleurotus ostreatus, the oyster mushroom, is a preferential lignin degrader occupying niches rich in lignocellulose such as decaying trees. here, we provide direct, genetically based proof for the functional significance of mnp to p. ostreatus ligninolytic capacity und ...201424119015
decontamination of a municipal landfill leachate from endocrine disruptors using a combined sorption/bioremoval approach.sorption and biodegradation are the main mechanisms for the removal of endocrine disruptor compounds (eds) from both solid and liquid matrices. there are recent evidences about the capacity of white-rot fungi to decontaminate water systems from phenolic eds by means of their ligninolytic enzymes. most of the available studies report the removal of eds by biodegradation or adsorption separately. this study assessed the simultaneous removal of five eds—the xenoestrogens bisphenol a (bpa), ethynile ...201424122163
release of feruloylated oligosaccharides from wheat bran through submerged fermentation by edible mushrooms.wheat bran, a by-product of the flour industry, is believed to be a raw material for the production of feruloylated oligosaccharides (fos) because of its high content of conjiont ferulic acid (fa). studies were carried out to identify edible mushrooms that are able to release fos from wheat bran. all the six tested mushrooms (pleurotus ostreatus, hericium erinaceum, auricularia auricula, cordyceps militaris, agrocybe chaxingu, and ganoderma lucium) were found to release fos, and agrocybe chaxing ...201424123163
experimental evaluation of analgesic and anti-inflammatory potential of oyster mushroom pleurotus florida. 201524130402
comparative assessment of three ligninolytic fungi for removal of phenolic endocrine disruptors from freshwaters and sediments.bisphenol a (bpa) and 4-n-nonylphenol (np) are two endocrine disruptor compounds dangerous to animals, especially aquatics, and humans. they can be leached from urban and industrial wastes and contaminate the environment. white rot fungi produce ligninolytic enzymes capable of biodegrading aromatic contaminants, including some endocrine disruptors. this investigation has evaluated the potential of three fungal species, trametes versicolor, stereum hirsutum and pleurotus ostreatus, to remove bpa ...201324191495
the white-rot fungus pleurotus ostreatus transformant overproduced intracellular camp and laccase.transformation of pleurotus ostreatus pc9 with the mutated heterotrimeric g protein alpha subunit (gα) gene resulted in higher laccase (lac) activity and intracellular cyclic adenosine monophosphate (camp) concentrations as compared to those in wild-type pc9. the transformant also exhibited higher lac activity than the wild type when cultured in a medium containing known lac inducers cuso4 and ferulic acid.201324200784
storage temperature and uv-irradiation influence on the ergosterol content in edible mushrooms.ergosterol (5,7,22-ergostatrien-3β-ol) and ergosteryl derivatives from different genera of edible mushrooms were separated and quantified by an isocratic reversed-phase high performance liquid chromatography (hplc) method. the technique allowed a rapid separation of free ergosterol and two ergosteryl derivatives occurring in mushrooms. the ergosterol content varied considerably depending on the fungus. thus, the species agaricus bisporus and hygrophorus marzuolus presented high quantities of erg ...201424206714
permeability characteristics of cell-membrane pores induced by ostreolysin a/pleurotolysin b, binary pore-forming proteins from the oyster mushroom.proteins from the oyster mushroom, 15 kda ostreolysin a (olya), and 59 kda pleurotolysin b (plyb) with a membrane attack complex/perforin (macpf) domain, damage cell membranes as a binary cytolytic pore-forming complex. measurements of single-channel conductance and transmembrane macroscopic current reveal that olya/plyb form non-selective ion-conducting pores with broad, skewed conductance distributions in n18 neuroblastoma and cho-k1 cell membranes. polyethylene-glycol 8000 (hydrodynamic radiu ...201424211835
yield and size of oyster mushroom grown on rice/wheat straw basal substrate supplemented with cotton seed hull.oyster mushroom (pleurotus ostreatus) was cultivated on rice straw basal substrate, wheat straw basal substrate, cotton seed hull basal substrate, and wheat straw or rice straw supplemented with different proportions (15%, 30%, and 45% in rice straw substrate, 20%, 30%, and 40% in wheat straw substrate) of cotton seed hull to find a cost effective substrate. the effect of autoclaved sterilized and non-sterilized substrate on growth and yield of oyster mushroom was also examined. results indicate ...201324235869
purification and characterization of a novel fibrinolytic enzyme from culture supernatant of pleurotus ostreatus.a fibrinolytic enzyme was produced by an edible mushroom of pleurotus ostreatus using submerged culture fermentation. the enzyme was purified from the culture supernatant by applying a combination of freeze-thaw treatment, ammonium sulfate precipitation, hydrophobic interaction, and gel filtration chromatographies. the enzyme was purified by a 147-fold, with a yield of 7.54%. the molecular masses of the enzyme an determined by gel filtration and sdspage were 13.6 and 18.2 kda, respectively. the ...201424248089
biotransformation of the antibiotic agent flumequine by ligninolytic fungi and residual antibacterial activity of the transformation mixtures.flumequine, a fluoroquinolone antibiotic, is applied preferably in veterinary medicine, for stock breeding and treatment of aquacultures. formation of drug resistance is a matter of general concern when antibiotics such as flumquine occur in the environment. thus, biodegradation of flumequine in solution was investigated using five different ligninolytic fungi. irpex lacteus, dichomitus squalens, and trametes versicolor proved most efficient and transformed more than 90% of flumequine within 6 o ...201324261869
anti-inflammatory effects of five commercially available mushroom species determined in lipopolysaccharide and interferon-γ activated murine macrophages.inflammation is a well-known contributing factor to many age-related chronic diseases. one of the possible strategies to suppress inflammation is the employment of functional foods with anti-inflammatory properties. edible mushrooms are attracting more and more attention as functional foods since they are rich in bioactive compounds, but their anti-inflammatory properties and the effect of food processing steps on this activity has not been systematically investigated. in the present study, whit ...201424262531
potential enrichment of medicinal mushrooms with selenium to obtain new dietary supplements.the purpose of this study was to resolve the question of whether various selenium concentrations affect the ability of eight species, members of five genera, to produce mycelium biomass and absorb this trace element during submerged cultivation in na2seo3-enriched medium. the obtained results demonstrated the ability of mycelia of all of the tested species to absorb selenium at analyzed concentrations. thus, selenium content ranged from 1.4 µg g-1 (pleurotus eryngii) to 20.3 µg g-1 (pleurotus os ...201324266370
optimization of submerged fermentation conditions for lovastatin production by the culinary-medicinal oyster mushroom, pleurotus ostreatus (higher basidiomycetes).statistical experimental designs were used to optimize lovastatin production by culinary-medicinal oyster mushroom pleurotus ostreatus obcc 1031 under submerged fermentation. the plackett-burman design was used to determine effective culture parameters, glucose, lactose, maltose, glycerol, peptone, yeast extract, nh4so2, nacl, thiamine, and agitation speed. statistical analyses of data from the plackett-burman design show that glucose, yeast extract, and agitation speed are significant parameter ...201324266373
antibacterial activity of mediterranean oyster mushrooms, species of genus pleurotus (higher basidiomycetes).extracts of the mediterranean culinary-medicinal oyster mushrooms pleurotus eryngii var. eryngii, p. eryngii var. ferulae, p. eryngii var. elaeoselini, and p. nebrodensis were tested for their in vitro growth inhibitory activity against a group of bacterial reference strains of medical relevance: staphylococcus aureus atcc 25923, s. epidermidis rp62a, pseudomonas aeruginosa atcc 15442, and escherichia coli atcc10536. all of the pleurotus species analyzed inhibited the tested microorganisms in va ...201324266382
hydrophobin-coated plates as matrix-assisted laser desorption/ionization sample support for peptide/protein analysis.fungal hydrophobins are amphipathic self-assembling proteins. vmh2 hydrophobin, prepared from mycelial cultures of the basidiomycete fungus pleurotus ostreatus, spontaneously forms a stable and homogeneous layer on solid surfaces and is able to strongly absorb proteins even in their active forms. in this work, we have exploited the vmh2 self-assembled layer as a novel coating of a matrix-assisted laser desorption/ionization (maldi) steel sample-loading plate. mixtures of standard proteins, as we ...201424287080
dynamics of sterols and fatty acids during uv-b treatment of oyster mushroom.fruiting bodies of the oyster mushroom pleurotus ostreatus were illuminated with uv-b with a light intensity maximum at 310-320 nm and 11.5 w/m² for 60 min at 20 °c. changes of the sterol and fatty acid spectrum were quantified. the onset of ergocalciferol (vitamin d₂) formation was immediate in fruiting bodies illuminated from the lamella side, in sliced fruiting bodies, and in the stipes. saturation concentrations above 100 μg/g of dry matter were reached after 1h. at the same time, the concen ...201424295670
antifungal activity of cinnamic acid derivatives involves inhibition of benzoate 4-hydroxylase (cyp53).cyp53a15, from the sorghum pathogen cochliobolus lunatus, is involved in detoxification of benzoate, a key intermediate in aromatic compound metabolism in fungi. because this enzyme is unique to fungi, it is a promising drug target in fungal pathogens of other eukaryotes.201424314266
cloning, expression, and characterization of a milk-clotting aspartic protease gene (po-asp) from pleurotus ostreatus.an aspartic protease gene from pleurotus ostreatus (po-asp) had been cloned based on the 3' portion of cdna in our previous work. the po-asp cdna contained 1,324 nucleotides with an open reading frame (orf) of 1,212 bp encoding 403 amino acid residues. the putative amino acid sequence included a signal peptide, an activation peptide, two most possible n-glycosylation sites and two conserved catalytic active site. the mature polypeptide with 327 amino acid residues had a calculated molecular mass ...201424338250
comparison of the interleukin-1β-inducing potency of allergenic spores from higher fungi (basidiomycetes) in a cryopreserved human whole blood system.spores from basidiomycete fungi (basidiospores) are highly prevalent in the atmosphere of urban and rural settings. studies have confirmed their potential to affect human health as allergens. less is known about their potential to serve as stimuli of the innate immune system and induce proinflammatory reactions.201424356469
ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability.the genome of pleurotus ostreatus, an important edible mushroom and a model ligninolytic organism of interest in lignocellulose biorefineries due to its ability to delignify agricultural wastes, was sequenced with the purpose of identifying and characterizing the enzymes responsible for lignin degradation.201424387130
pleurotus ostreatus, an edible mushroom, enhances glucose 6-phosphate dehydrogenase, ascorbate peroxidase and reduces xanthine dehydrogenase in major organs of aged rats.aging is now considered to be associated with an elevation in oxidative damage to macromolecules and enhanced levels of inflammation. therefore, inhibition of age-related oxidative stress by natural supplement is an important study.201424392756
pressurized water extraction of β-glucan enriched fractions with bile acids-binding capacities obtained from edible mushrooms.a pressurized water extraction (pwe) method was developed in order to extract β-glucans with bile acids-binding capacities from cultivated mushrooms (agaricus bisporus, lentinula edodes, and pleurotus ostreatus) to be used as supplements to design novel foods with hypocholesterolemic properties. extraction yields were higher in individual than sequential extractions being the optimal extraction parameters: 200°c, 5 cycles of 5 min each at 10.3 mpa. the crude polysaccharide (psc) fractions, isola ...024399760
pleurotus ostreatus - breeding potential of a new cultivated mushroom.two isolates of pleurotus ostreatus from north america and one from germany are interbreedable. under identical conditions at low temperatures, their fruiting bodies are hard to distinguish. however, shape and colour and several other characters vary with culture conditions. the american stocks fruit well at temperatures from 4 to 24 °c, the german ones only below 15 °c. four types of hybrids between german and american pleupotus were obtained: i) the whole fruiting process is temperature sensit ...197624414617
intergeneric hybridization between pleurotus ostreatus and schizophyllum commune by peg-induced protoplast fusion.intergeneric hybridization between pleurotus ostreatus and schizophyllum commune was studied using peg-induced fusion. the fusion of protoplasts from auxotrophic mutant strains resulted in the formation of fusion hybrids in the frequencies of 3.6 to 7.3×10(-5). most of these fusion hybrids were monokaryotic and sterile and no heterokaryosis occurred. most fusants showed a significantly higher nuclear dna content when compared to parental strains and no diploids (parent 1 genome plus parent 2 gen ...199624415417
crystallization and preliminary x-ray crystallographic analysis of the small subunit of the heterodimeric laccase poxa3b from pleurotus ostreatus.laccases are multicopper oxidases of great biotechnological potential. while laccases are generally monomeric glycoproteins, the white-rot fungus pleurotus ostreatus produces two closely related heterodimeric isoenzymes composed of a large subunit, homologous to the other fungal laccases, and a small subunit. the sequence of the small subunit does not show significant homology to any other protein or domain of known function and consequently its function is unknown. the highest similarity to pro ...201324419623
effect of crude mansonia (mansonia altisima) timber extracts on biodeterioration of obeche (triplochiton scleroxylon) timber by three wood-rotting fungi.methanol and ethanol extracts of mansonia wood inhibited the growth of three wood-rotting fungi, pleurotus ostreatus, gloeophyllum sepiarium and gloeophyllum sp. an aqueous extract only inhibited the growth of p. ostreatus. extracted obeche wood impregnated with ethanol extracts of mansonia showed significant improvement in decay resistance. impregnation with methanol extracts only significantly retarded decay by p. ostreatus and the aqueous extracts did not increase obeche wood resistance to an ...199424421183
culture media statistical optimization for biomass production of a ligninolytic fungus for future rice straw degradation.the main objective of this study was to optimize a culture media for low scale biomass production of pleurotus spp. future applications of this optimization will be implemented for "in situ" rice straw degradation, increase soil nutrients availability, and lower residue and rice culture management costs. soil samples were taken from different points in six important rice production cities in colombia. for carbon and nitrogen source selection a factorial 4(2) design was carried out. the plackett- ...201324426109
thiabendazole uptake in shimeji, king oyster, and oyster mushrooms and its persistence in sterile and nonsterile substrates.thiabendazole in the substrates incurred from spraying and premixing was translocated to the pileus, stipe, and volva of selected mushrooms. the spraying on the substrates resulted in higher residues of thiabendazole in all three mushrooms than the premixing treatment. for premixing, in the five substrates, half-lives of thiabendazole were found to be 13.6 days for shimeji, 10.0 days for king oyster, 13.7 days for oyster, 19.1 days for sterilized substrate, and 8.4 days for nonsterilized substra ...201424432721
effective mutations in a high redox potential laccase from pleurotus ostreatus.since the first report on a laccase, there has been a notable development in the interest towards this class of enzymes, highlighted from the number of scientific papers and patents about them. at the same time, interest in exploiting laccases-mainly high redox potential-for various functions has been growing exponentially over the last 10 years. despite decades of work, the molecular determinants of the redox potential are far to be fully understood. for this reason, interest in tuning laccase ...201424463760
box-behnken methodology for cr (vi) and leather dyes removal by an eco-friendly biosorbent: f. vesiculosus.this study focused on leather industrial effluents treatment by biosorption using fucus vesiculosus as low-cost adsorbent. these effluents are yellowish-brown color and high concentration of cr (vi). therefore, biosorption process was optimized using response surface methodology based on box-behnken design operating with a simulated leather effluent obtained by mixture of cr (vi) solution and four leather dyes. the key variables selected were initial solution ph, biomass dosage and cacl2 concent ...201424484851
efficient recovery of lignocellulolytic enzymes of spent mushroom compost from oyster mushrooms, pleurotus spp., and potential use in dye decolorization.this study was conducted in order to perform efficient extraction of lignocellulolytic enzymes amylase (ec 3.2.1.1), cellulase (ec 3.2.1.4), laccase (ec 1.10.3.2), and xylanase (ec 3.2.1.8) from spent mushroom compost (smc) of pleurotus ostreatus, p. eryngii, and p. cornucopiae. optimal enzyme recovery was achieved when smcs were extracted with 50 mm sodium citrate (ph 4.5) buffer at 4℃ for 2 hr. amylase, cellulase, and xylanase activities showed high values in extracts from p. ostreatus smc, wi ...201324493942
isolation of fungal pathogens to an edible mushroom, pleurotus eryngii, and development of specific its primers.fungal pathogens have caused severe damage to the commercial production of pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. four strains of pathogenic fungi, including trichoderma koningiopsis dc3, phomopsis sp. mp4, mucor circinelloides mp5, and cladosporium bruhnei mp6, were isolated from the bottle culture of diseased p. eryngii. a species-specific primer set was designed for each fungus from the i ...201324493949
the perforin pore facilitates the delivery of cationic cargos.cytotoxic lymphocytes eliminate virally infected or neoplastic cells through the action of cytotoxic proteases (granzymes). the pore-forming protein perforin is essential for delivery of granzymes into the cytoplasm of target cells; however the mechanism of this delivery is incompletely understood. perforin contains a membrane attack complex/perforin (macpf) domain and oligomerizes to form an aqueous pore in the plasma membrane; therefore the simplest (and best supported) model suggests that gra ...201424558045
ligninolytic peroxidase gene expression by pleurotus ostreatus: differential regulation in lignocellulose medium and effect of temperature and ph.pleurotus ostreatus is an important edible mushroom and a model lignin degrading organism, whose genome contains nine genes of ligninolytic peroxidases, characteristic of white-rot fungi. these genes encode six manganese peroxidase (mnp) and three versatile peroxidase (vp) isoenzymes. using liquid chromatography coupled to tandem mass spectrometry, secretion of four of these peroxidase isoenzymes (vp1, vp2, mnp2 and mnp6) was confirmed when p. ostreatus grows in a lignocellulose medium at 25°c ( ...201424560615
development of an improved variant of gh51 α-l-arabinofuranosidase from pleurotus ostreatus by directed evolution.in this study, the α-l-arabinofuranosidase from pleurotus ostreatus was subjected to directed evolution by expressing a library of around 7000 randomly mutated variants by error prone polymerase chain reaction. high-throughput screening of the library for the most active variants was performed by assaying activity towards p-nitrophenyl α-l-arabinofuranoside, and a variant with higher activity than the wild type was selected, purified and characterised. it exhibited a kcat of 7.3 ×1 0¹ ± 0.3 min⁻ ...201424565609
toward an understanding of the effects of agitation and aeration on growth and laccases production by pleurotus ostreatus.mycelial growth and laccase production by pleurotus ostreatus cp50 cultured in a 10-l mechanically agitated bioreactor were assessed through a 2(3) factorial experimental design. the main effects and interactions of three factors (agitation, aeration and copper induction) over five responses (μ, αlacc, βlacc, maximal volumetric laccase activity and maximal biomass concentration) were analyzed. p. ostreatus growth was significantly improved when culturing was conducted with high agitation (5.9kw/ ...201424572371
microsatellites in the genome of the edible mushroom, volvariella volvacea.using bioinformatics software and database, we have characterized the microsatellite pattern in the v. volvacea genome and compared it with microsatellite patterns found in the genomes of four other edible fungi: coprinopsis cinerea, schizophyllum commune, agaricus bisporus, and pleurotus ostreatus. a total of 1346 microsatellites have been identified, with mono-nucleotides being the most frequent motif. the relative abundance of microsatellites was lower in coding regions with 21 no./mb. howeve ...201424575404
molecular divergence and species delimitation of the cultivated oyster mushrooms: integration of igs1 and its.identification of edible mushrooms particularly pleurotus genus has been restricted due to various obstacles. the present study attempted to use the combination of two variable regions of igs1 and its for classifying the economically cultivated pleurotus species. integration of the two regions proved a high ability that not only could clearly distinguish the species but also served sufficient intraspecies variation. phylogenetic tree (igs1+its) showed seven distinct clades, each clade belonging ...201424587752
determination of statins by gas chromatography - ei/mrm - tandem mass spectrometry: fermentation of pine samples with pleurotus ostreatus.statins were separated and quantified with gas chromatography-mass spectrometry (gc-ei-ms/ms) using total ion monitoring (tic) and multiple reactions monitoring (mrm). the mrm method in statins determination has a novelty value, since there are no previous studies on their simultaneous analysis in environmental or plant samples. the method development and optimization was challenging due to the physicochemical similarities of the silylated lovastatin, simvastatin, pravastatin, fluvastatin, and a ...201424607418
biosorption of malachite green from aqueous solutions by pleurotus ostreatus using taguchi method.dyes released into the environment have been posing a serious threat to natural ecosystems and aquatic life due to presence of heat, light, chemical and other exposures stable. in this study, the pleurotus ostreatus (a macro-fungus) was used as a new biosorbent to study the biosorption of hazardous malachite green (mg) from aqueous solutions. the effective disposal of p. ostreatus is a meaningful work for environmental protection and maximum utilization of agricultural residues.the operational p ...201424620852
effects of uv-b radiation levels on concentrations of phytosterols, ergothioneine, and polyphenolic compounds in mushroom powders used as dietary supplements.compositional changes of powder dietary supplements made from mushrooms exposed to different levels of uv-b irradiation were evaluated for the bioactive naturally occurring mushroom antioxidant, ergothioneine; other natural polyphenolic compounds, e.g., flavonoids, lignans, etc.; and selected phytosterols. four types of mushroom powder consisting of white, brown (agaricus bisporus), oyster (pleurotus ostreatus), and shiitake (lentinula edodes) mushrooms from three different treatment groups (con ...201424628700
evaluation of the cytotoxic effects of cam therapies: an in vitro study in normal kidney cell lines.the purpose of this current study was to justify the incorporation of complementary and alternate medicine (cam) in current cancer treatments. the major drawback of anticancer drugs is their nonselective killing, which ultimately leads to attrition of normal cells. keeping this as the foundation of our study, we made an effort to compare the cytotoxicity associated with a known chemotherapeutic drug 5-fluorouracil (5-fu), with certain cam therapies previously reported to have anticancer activity ...201424672319
statistical optimization of ultraviolet irradiate conditions for vitamin d₂ synthesis in oyster mushrooms (pleurotus ostreatus) using response surface methodology.response surface methodology (rsm) was used to determine the optimum vitamin d2 synthesis conditions in oyster mushrooms (pleurotus ostreatus). ultraviolet b (uv-b) was selected as the most efficient irradiation source for the preliminary experiment, in addition to the levels of three independent variables, which included ambient temperature (25-45°c), exposure time (40-120 min), and irradiation intensity (0.6-1.2 w/m2). the statistical analysis indicated that, for the range which was studied, i ...201424736742
mn²⁺-deficiency reveals a key role for the pleurotus ostreatus versatile peroxidase (vp4) in oxidation of aromatic compounds.the manganese peroxidase gene family (mnps) is a part of the ligninolytic system of pleurotus ostreatus. this gene family is comprised of nine members, mnp1-9, encoding short manganese peroxidases (short-mnps) or versatile peroxidases (vps). we show that unlike in mn(2+)-amended glucose-peptone (gp) medium, where redundancy among mnps was reported, in mn(2+)-deficient gp medium mnp4 [encoding versatile peroxidase isoenzyme 4 (vp4)] has a key and nonredundant function. the abundance of mnps trans ...201424737058
over-expression of psgpd, a mushroom glyceraldehyde-3-phosphate dehydrogenase gene, enhances salt tolerance in rice plants.transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (gpd), isolated from the oyster mushroom, pleurotus sajor-caju, had increased tolerance to salt stress (jeong et al. biochem biophys res commun 278:192-196, 2000). to examine the physiological mechanisms enhancing salt tolerance in gpd-transgenic rice plants, the salt tolerance of five gpd transgenic rice lines (t1-t5) derived from dongjin rice cultivar were evaluated in a fixed 150 mm saline environment in comparison to two ...201424737077
[protective activity of aqueous extracts from higher mushrooms against herpes simplex virus type-2 on albino mice model].toxicity and antiviral activity of aqueous extracts from higher mushrooms such as lentinula edodes (berk.) pegler (shiitake), pleurotus ostreatus (jacq.) p. kumm. (oyster), inonotus obliquus (ach. ex pers.) pilát (chaga), hydnellum compactum (pers.) p. karst. (compact tooth) were studied. in doses of 0.8 to 4.0 mg (dry weight) per mouse administered orally or intraperitoneally the extracts showed no acute toxicity. when the dose of the chaga extract was increased to 20 mg per mouse, a half of th ...201324738237
antitumor activity of pleurotus ostreatus polysaccharide fractions on ehrlich tumor and sarcoma 180.the medicinal properties of fungi of the genus pleurotus have attracted great interest due to their therapeutic properties. polysaccharides synthesized by pleurotus, including the β-glucans are considered the main responsible for its therapeutic properties. this study aimed to evaluate the efficacy of polysaccharidic fractions extracted from mycelial biomass of pleurotus ostreatus dsm 1833 in inhibiting the development of ehrlich tumor (et) and sarcoma 180 (s-180). fc, fi and fii fractions provi ...201424768967
biodegradation of aflatoxin b1 in contaminated rice straw by pleurotus ostreatus mtcc 142 and pleurotus ostreatus ghbbf10 in the presence of metal salts and surfactants.aflatoxin b1 (afb1) is a highly toxic fungal metabolite having carcinogenic, mutagenic and teratogenic effects on human and animal health. accidental feeding of aflatoxin-contaminated rice straw may be detrimental for ruminant livestock and can lead to transmission of this toxin or its metabolites into the milk of dairy cattle. white-rot basidiomycetous fungus pleurotus ostreatus produces ligninolytic enzymes like laccase and manganese peroxidase (mnp). these extracellular enzymes have been repo ...201424770873
isolation, culture optimization and physico-chemical characterization of laccase enzyme from pleurotus fossulatus.pleurotus fossulatus (cooke) sace is member of oyster mushroom can produced extracellular laccase (benzenediol: oxygen oxidoreductase; ec 1.10.3.2) in submerged fermentation. to analyze the optimum production for laccase p. fossulatus was cultured both in stationary and shaking condition in different media. partial purification of laccase was done after 0-80% ammonium sulphate precipitation, followed by deae (diethylaminoethyl) sephadex (a-50) anion exchange chromatography. potato-sucrose pepton ...201424783799
evaluation of biomass of some invasive weed species as substrate for oyster mushroom (pleurotus spp.) cultivation.this study assessed the bioconversion of agriculture wastes like invasive weeds species (lantana camara, prosopis juliflora, parthenium hysterophorus) as a substrate for oyster mushroom (pleurotus species) cultivation together with wheat straw as a control. the experiment was laid out in factorial combination of substrates and three edible oyster mushroom species in a completely randomized design (crd) with three replications. pleurotus ostreatus gave significantly (p < 0.01) total yield of 840 ...201424783804
fungal macpf-like proteins and aegerolysins: bi-component pore-forming proteins?proteins with membrane-attack complex/perforin (macpf) domains are found in almost all kingdoms of life, and they have a variety of biological roles, including defence and attack, organism development, and cell adhesion and signalling. the distribution of these proteins in fungi appears to be restricted to some pezizomycotina and basidiomycota species only, in correlation with another group of proteins with unknown biological function, known as aegerolysins. these two protein groups coincide in ...201424798017
bioconversion of lignocellulosic residues by agrocybe cylindracea and pleurotus ostreatus mushroom fungi--assessment of their effect on the final product and spent substrate properties.nine agro-industrial and forestry by-products were subjected to solid-state fermentation by agrocybe cylindracea and pleurotus ostreatus, and the process and end-products were comparatively evaluated. grape marc waste plus cotton gin trash was the best performing medium for both fungi, while substrate composition had a marked effect on most cultivation parameters. biological efficiency was positively correlated with nitrogen, lignin and ash, and negatively with hemicelluloses and carbohydrate co ...201424837930
biodegradation of heptachlor and heptachlor epoxide-contaminated soils by white-rot fungal inocula.the ability of certain white-rot fungi (wrf) inocula to transform heptachlor and heptachlor epoxide and its application in artificially contaminated soil were investigated. fungal inoculum of pleurotus ostreatus eliminated approximately 89 % of heptachlor after 28 days of incubation, and chlordene was detected as the primary metabolite. the fungal inoculum of pleurotus ostreatus had the highest ability to degrade heptachlor epoxide; approximately 32 % were degraded after 28 days of incubation, a ...201424840358
comparative evaluation of polysaccharides isolated from astragalus, oyster mushroom, and yacon as inhibitors of α-glucosidase.the incidence of diabetes has increased considerably, and become the third serious chronic disease following cancer and cardiovascular diseases. though acarbose, metformin, and 1-deoxynojirimycin have good efficacy for clinical application as hypoglycemic drugs, their expensive costs and some degree of side effects have limited their clinical application. recently, increasing attention has concentrated on the polysaccharides from natural plant and animal sources for diabetes. in order to illustr ...201424863354
x-ray crystallographic structure of rnase po1 that exhibits anti-tumor activity.rnase po1 is a guanylic acid-specific ribonuclease member of the rnase t1 family from pleurotus ostreatus. we previously reported that rnase po1 inhibits the proliferation of human tumor cells, yet rnase t1 and other t1 family rnases are non-toxic. we determined the three-dimensional x-ray structure of rnase po1 and compared it with that of rnase t1. the catalytic sites are conserved. however, there are three disulfide bonds, one more than in rnase t1. one of the additional disulfide bond is in ...201424882409
screening of indigenously isolated fungi for lovastatin production and its in vivo evaluation.seven indigenously isolated fungal strains (aspergillus niger, aspergillus flavus, aspergillus terreus, penicillium citrinum, penicillium notatum, pleurotus ostreatus and trichoderma viradae) were tested for their potential to produce cholesterol lowering drug lovastatin by using different agro-industrial wastes (corn cobs, corn stover, banana stalk, wheat straw, wheat bran, bagasse) in submerged as well as solid state fermentation. aspergillus terreus showed maximum production of 18.74 mg/100 m ...201424894549
immobilization of a pleurotus ostreatus laccase mixture on perlite and its application to dye decolourisation.in the present study, a crude laccase preparation from pleurotus ostreatus was successfully immobilized on perlite, a cheap porous silica material, and tested for remazol brilliant blue r (rbbr) decolourisation in a fluidized bed recycle reactor. results showed that rbbr decolourisation is mainly due to enzyme action despite the occurrence of dye adsorption-related enzyme inhibition. fine tuning of immobilization conditions allowed balancing the immobilization yield and the resulting rate of dec ...201424895564
characteristic odorants from bailingu oyster mushroom (pleurotus eryngii var. tuoliensis) and summer oyster mushroom (pleurotus cystidiosus).in this study, the characteristic odorants of the volatile oils from pleurotus species (p. eryngii var. tuoliensis and p. cystidiosus) were extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (gc-ms), gas chromatography-olfactometry (gc-o), and aroma extract dilution analysis (aeda). a total of 52 and 54 components (p. eryngii var. tuoliensis and p. cystidiosus, respectively) were identified, representing about 98.8% and 85.1% of the volatile oils, respectively. t ...201424919476
telomerase inhibitory effects of medicinal mushrooms and lichens, and their anticancer activity.telomerase has been widely accepted as a cancer marker and a promising therapeutic target for novel anticancer drugs. the aim of this study was to investigate the in vitro telomerase inhibitory effects of mushrooms and their anticancer properties. the inhibitory effects of mushrooms and lichens against telomerase activity of hl-60 cells were systematically assessed using polymerase chain reaction based on assay of telomeric repeat amplification protocol. telomerase inhibitory samples were furthe ...201424940901
the effects of the king oyster mushroom pleurotus eryngii (higher basidiomycetes) on glycemic control in alloxan-induced diabetic mice.the purpose of this study is to investigate the effects of pleurotus eryngii on glycemic metabolism. alloxan-induced hyperglycemic mice were used to study the effects of p. eryngii on blood glucose, glycohemoglobin, insulin secretion, damaged pancreatic β-cells, total antioxidant status (taos), and hepatic glycogen in hyperglycemic mice. sixty diabetic mice were divided equally into 5 groups: the alloxan (ax)-induced hyperglycemic group, the ax and glibenclamide (gli)-treated group, the ax and p ...201424941163
medicinal and antimicrobial role of the oyster culinary-medicinal mushroom pleurotus ostreatus (higher basidiomycetes) cultivated on banana agrowastes in india.oyster mushrooms, species of the genus pleurotus, are recognized for producing secondary metabolites with important medicinal properties. investigations were carried out to evaluate the antioxidative and antimicrobial properties of the edible mushroom pleurotus ostreatus (mtcc142) extracts cultivated on banana agrowastes. ethanolic extracts showed antimicrobial activities against gram-positive and gram-negative bacteria, and their in vitro antifungal activities against all fungi tested revealed ...201424941164
chemical composition and nutritional and medicinal value of fruit bodies and submerged cultured mycelia of culinary-medicinal higher basidiomycetes mushrooms.this research gives the results of a proximate analysis (moisture, ash, crude protein, fat, total carbohydrates, and total energy); a bioactive compounds analysis (γ-aminobutyric acid [gaba], ergothioneine, lovastatin, and cordycepin); fatty acid and amino acid analysis; and an analysis of macro- and microelement content of fruit bodies and mycelia of 15 higher basidiomycetes medicinal mushroom strains belonging to 12 species. the results obtained demonstrate that almost all investigated mushroo ...201424941169
anti-tumor and macrophage activation induced by alkali-extracted polysaccharide from pleurotus ostreatus.pleurotus ostreatus is popularly consumed as traditional medicine and health food for enhancing immune function in china. polysaccharides from mushroom have been demonstrated to possess a wide range of health beneficial properties. this study was carried out to elucidate the immunomodulating effects and molecular mechanism involved in the in vivo and in vitro anti-tumor activities of alkali-extracted polysaccharide (wpop-n1) from the fruiting bodies of p. ostreatus. the results showed that wpop- ...201424942990
aflatoxin detoxification by manganese peroxidase purified from pleurotus ostreatus.manganese peroxidase (mnp) was produced from white rot edible mushroom pleurotus ostreatus on the culture filtrate. the enzyme was purified to homogeneity using (nh4)2so4 precipitation, deae-sepharose and sephadex g-100 column chromatography. the final enzyme activity achieved 81 u ml(-1), specific activity 78 u mg(-1) with purification fold of 130 and recovery 1.2% of the crude enzyme. sds-page indicated that the pure enzyme have a molecular mass of approximately 42 kda. the optimum ph was betw ...201424948923
high removal of chemical and biochemical oxygen demand from tequila vinasses by using physicochemical and biological methods.the goal of this research is to find a more effective treatment for tequila vinasses (tvs) with potential industrial application in order to comply with the mexican environmental regulations. tvs are characterized by their high content of solids, high values of biochemical oxygen demand (bods), chemical oxygen demand (cod), low ph and intense colour; thus, disposal of untreated tvs severely impacts the environment. physicochemical and biological treatments, and a combination of both, were probed ...201424956770
potential of fungal co-culturing for accelerated biodegradation of petroleum hydrocarbons in soil.the potential of fungal co-culture of the filamentous pestalotiopsis sp. ng007 with four different basidiomycetes--trametes versicolor u97, pleurotus ostreatus pl1, cerena sp. f0607, and polyporus sp. s133--for accelerating biodegradation of petroleum hydrocarbons (phcs) was studied using three different physicochemical characteristic phcs in soil. all the combinations showed a mutual intermingling mycelial interaction on the agar plates. however, only ng007/s133 (50/50) exhibited an optimum gro ...201424997261
potential of pleurotus ostreatus mycelium for selenium absorption.the aim of this study was to evaluate the effect of high selenium (se) concentrations on morphophysiological and ultrastructural properties of pleurotus ostreatus. mycelium growth was good in media enriched with 5.0, 10.0, and 20.0 mg l(-1) of se, concentration of 500.0 mg l(-1) strongly inhibited growth, and 1000.0 mg l(-1) was the minimum inhibitory concentration. contrary to thin-walled, hyaline, branched, and anastomized hyphae with clamp-connections in the control, at se concentrations of 1 ...201425003145
water-soluble polysaccharides from pleurotus ostreatus var. florida mycelial biomass.pleurotus ostreatus var. florida known as hiratake has a high nutritional value, presents medicinal and nutraceutical properties and it is one of the consumed mushrooms in brazil. thus, the aim of this study was to characterize the chemical structure of polysaccharides found in mycelial biomass produced by submerged culture of p. ostreatus var. florida in order to compare with those found in p. ostreatus var. florida fruit bodies. aqueous and alkali extracts obtained from mycelial biomass were p ...201425008131
the effect of pleurotus ostreatus arabinofuranosidase and its evolved variant in lignocellulosic biomasses conversion.the fungal arabinofuranosidase from pleurotus ostreatus poabf recombinantly expressed in pichia pastoris rpoabf and its evolved variant rpoabf f435y/y446f were tested for their effectiveness to enhance the enzymatic saccharification of three lignocellulosic biomasses, namely arundo donax, corn cobs and brewer's spent grains (bsg), after chemical or chemical-physical pretreatment. all the raw materials were subjected to an alkaline pretreatment by soaking in aqueous ammonia solution whilst the bi ...201425046861
nutrient digestibility, ruminal fermentation activities, serum parameters and milk production and composition of lactating goats fed diets containing rice straw treated with pleurotus ostreatus.the study evaluated replacement of egyptian berseem clover (bc, trifolium alexandrinum) with spent rice straw (srs) of pleurotus ostreatus basidiomycete in diets of lactating baladi goats. nine lactating homo-parity baladi goats (average bw 23.8±0.4 kg) at 7 d postpartum were used in a triplicate 3×3 latin square design with 30 d experimental periods. goats were fed a basal diet containing 0 (control), 0.25 (srs25) and 0.45 (srs45) (w/w, dm basis) of srs. the control diet was berseem clover and ...201425049962
biosynthesis and characterization of silver nanoparticles produced by pleurotus ostreatus and their anticandidal and anticancer activities.the biosynthesis of nanoparticles has received increasing interest because of the growing need to develop safe, cost-effective and environmentally friendly technologies for the synthesis of nano-materials. in this study, silver nanoparticles (agnps) were synthesized using a reduction of aqueous ag(+) ions with culture supernatant from pleurotus ostreatus. the bioreduction of agnps was monitored by ultra violet-visible spectroscopy and the obtained agnps were characterized by transmission electro ...201425053172
differential response of oyster shell powder on enzyme profile and nutritional value of oyster mushroom pleurotus florida pf05.oyster mushroom pleurotus florida was cultivated on different combinations of wheat straw (ws) as basal substrate and oyster shell powder (osp) supplement. the osp supplementation considerably responded to different cultivation phases. the mycelium grew fast and showed rapid growth rate (8.91 mmd(-1)) in ws + osp (97 + 3) combination while ws + osp (92 + 8) showed maximum laccase (3.133 u/g) and mn peroxidase (mnp) activities (0.091 u/g). the climax level of laccase (5.433 u/g) and mnp (0.097 u/ ...201425054140
high-level expression of a manganese superoxide dismutase (pomn-sod) from pleurotus ostreatus in pichia pastoris.the full-length cdna of pleurotus ostreatus superoxide dismutase (pomn-sod) was cloned and successfully expressed by using the ppic9k vector under the control of alcohol oxidase 1 promoter with a secretion signal peptide (α-factor) in pichia pastoris. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and western blotting demonstrated that recombinant pomn-sod, a 21.8 kda protein, was secreted into the culture medium. nondenaturing page experiments confirmed that recombinant po ...201425059984
overproduction of laccase by the white-rot fungus pleurotus ostreatus using apple pomace as inducer.laccase activity of pleurotus ostreatus is significantly increased by the addition of apple pomace. among various conditions, the best concentration of apple pomace and cultivation time for the production of laccase by p. ostreatus was 2.5% and 9 days, respectively. reverse transcription polymerase chain reaction analyses of laccase isoenzyme genes, including pox1, pox3, pox4, poxc, poxa3, and poxa1b, revealed a clear effect of apple pomace on transcription induction. our findings reveal that th ...201425071391
profile of natural redox mediators production of laccase-producing fungus pleurotus ostreatus.polycyclic aromatic hydrocarbons (pahs) are highly toxic organic pollutants which are abundant and environmentally widespread. anthracene is a simple pah that can be oxidized by laccases, copper-containing oxidase enzymes, produced by some plants, fungi, and bacteria. in this work, the extracellular culture fluid (cf) of laccase-producing fungus pleurotus ostreatus was separated to crude laccase (cl) and aqueous ultrafiltrate (au) fractions. the rate of anthracene oxidation by cf was 68.7 % whil ...201425108623
antioxidant properties of mushroom mycelia obtained by batch cultivation and tocopherol content affected by extraction procedures.the determination of the antioxidant potential of lyophilized mushroom mycelia from 5 strains of the species pleurotus ostreatus and coprinus comatus (obtained by submerged cultivation in batch system) was analyzed as ethanolic extracts by evaluating abts and the hydroxyl scavenging activity, frap method, the chelating capacity, the inhibition of human erythrocyte hemolysis, and the inhibition of xanthine oxidase activity. the main compounds present in all extracts were determined by hplc chroma ...201425110715
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