Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| fermentative production of ribonucleotides from whey by kluyveromyces marxianus: effect of temperature and ph. | ribonucleotides have shown many promising applications in food and pharmaceutical industries. the aim of the present study was to produce ribonucleotides (rna) by kluyveromyces marxianus atcc 8,554 utilizing cheese whey, a dairy industry waste, as a main substrate under batch fermentation conditions. the effects of temperature, ph, aeration rate, agitation and initial cellular concentration were studied simultaneously through factorial design for rna, biomass production and lactose consumption. ... | 2013 | 24426003 |
| diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid. | yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ... | 2014 | 24442819 |
| yeasts are essential for cocoa bean fermentation. | cocoa beans (theobroma cacao) are the major raw material for chocolate production and fermentation of the beans is essential for the development of chocolate flavor precursors. in this study, a novel approach was used to determine the role of yeasts in cocoa fermentation and their contribution to chocolate quality. cocoa bean fermentations were conducted with the addition of 200ppm natamycin to inhibit the growth of yeasts, and the resultant microbial ecology and metabolism, bean chemistry and c ... | 2014 | 24462702 |
| novel antihypertensive lactoferrin-derived peptides produced by kluyveromyces marxianus: gastrointestinal stability profile and in vivo angiotensin i-converting enzyme (ace) inhibition. | novel antihypertensive peptides released by kluyveromyces marxianus from bovine lactoferrin (lf) have been identified. k. marxianus lf permeate was fractionated by semipreparative high performance liquid chromatography and 35 peptides contained in the angiotensin i-converting enzyme (ace)-inhibitory fractions were identified by using an ion trap mass spectrometer. on the basis of peptide abundance and common structural features, six peptides were chemically synthesized. four of them (dpyklrp, py ... | 2014 | 24476136 |
| direct fermentation of raw starch using a kluyveromyces marxianus strain that expresses glucoamylase and alpha-amylase to produce ethanol. | raw starch and raw cassava tuber powder were directly and efficiently fermented at elevated temperatures to produce ethanol using the thermotolerant yeast kluyveromyces marxianus that expresses α-amylase from aspergillus oryzae as well as α-amylase and glucoamylase from debaryomyces occidentalis. among the constructed k. marxianus strains, yrl 009 had the highest efficiency in direct starch fermentation. raw starch from corn, potato, cassava, or wheat can be fermented at temperatures higher than ... | 2014 | 24478139 |
| permeabilization of kluyveromyces marxianus with mild detergent for whey lactose hydrolysis and augmentation of mixed culture. | cheese whey is a by-product of cheese-manufacturing industries, and the utilization of whey is a challenging problem either to use it or dispose it, because only few microorganisms can metabolize the whey lactose. enzymatic hydrolysis of whey lactose to glucose and galactose by β-galactosidase is the approach for biotechnological application. kluyveromyces marxianus cells were permeabilized with non-toxic, biodegradable, anionic detergent n-lauroyl sarcosine (n-ls) for the enzyme activity. the p ... | 2014 | 24500798 |
| a broad-range yeast expression system reveals arxula adeninivorans expressing a fungal self-sufficient cytochrome p450 monooxygenase as an excellent whole-cell biocatalyst. | the feasibility of using a single vector to clone a cytochrome p450 monooxygenase (p450) in different yeasts and then compare whole-cell hydroxylase activity was investigated. a broad-range yeast expression vector using the yltefp to drive expression of the cloned gene and the sctefp to drive the hygromycin resistance marker gene was used to clone the genes encoding two self-sufficient p450s, cyp102a1 and cyp505a1. both genes were cloned into saccharomyces cerevisiae, kluyveromyces marxianus, ya ... | 2014 | 24521080 |
| hydrolysis of agave fourcroydes lemaire (henequen) leaf juice and fermentation with kluyveromyces marxianus for ethanol production. | carbon sources for biofuel production are wide-ranging and their availability depends on the climate and soil conditions of the land where the production chain is located. henequen (agave fourcroydes lem.) is cultivated in yucatán, mexico to produce natural fibers from the leaves, and a juice containing fructans is produced during this process. fructans can be hydrolyzed to fructose and glucose and metabolized into ethanol by appropriate yeasts. in mexico, different agave species provide the car ... | 2014 | 24529165 |
| physiological characterization of thermotolerant yeast for cellulosic ethanol production. | the conversion of lignocellulose into fermentable sugars is considered a promising alternative for increasing ethanol production. higher fermentation yield has been achieved through the process of simultaneous saccharification and fermentation (ssf). in this study, a comparison was performed between the yeast species saccharomyces cerevisiae and kluyveromyces marxianus for their potential use in ssf process. three strains of s. cerevisiae were evaluated: two are widely used in the brazilian etha ... | 2014 | 24535257 |
| biosorption of lead by kluyveromyces marxianus immobilized in alginate beads. | the uptake and recovery of pb (ii) ions were investigated by using sodium alginate beads. biosorption experiments are carried out in batch mode. the experimental results showed that the beads were effective in removing pb (ii) ions from solution. biosorption equilibrium was approached within 16hrs. pseudo first order was applicable to all the sorption data over the entire time range. the sorption data conformed well for the langmuir isotherm model. the maximum adsorption capacity (qmax) onto alg ... | 2013 | 24558794 |
| modeling the microbial growth and temperature profile in a fixed-bed bioreactor. | aiming to scale up and apply control and optimization strategies, currently is required the development of accurate plant models to forecast the process nonlinear dynamics. in this work, a mathematical model to predict the growth of the kluyveromyces marxianus and temperature profile in a fixed-bed bioreactor for solid-state fermentation using sugarcane bagasse as substrate was built up. a parameter estimation technique was performed to fit the mathematical model to the experimental data. the es ... | 2014 | 24658796 |
| potential of some yeast strains in the stereoselective synthesis of (r)-(-)-phenylacetylcarbinol and (s)-(+)-phenylacetylcarbinol and their reduced 1,2-dialcohol derivatives. | whole cells of different yeast species have been widely used for a number of asymmetric transformations. in the present study, the screening of several yeast strains revealed the utility of debaryomyces etchellsii in acyloin condensation for (r)-(-)-phenylacetylcarbinol production. some conditions for the efficient biotransformation of benzaldehyde and minimization in the production of by-products were explored: ph of the reaction medium, use of additives (ethanol or acetonitrile), temperature, ... | 2014 | 24671162 |
| interactions between kluyveromyces marxianus and saccharomyces cerevisiae in tequila must type medium fermentation. | traditional tequila fermentation is a complex microbial process performed by different indigenous yeast species. usually, they are classified in two families: saccharomyces and non-saccharomyces species. using mixed starter cultures of several yeasts genera and species is nowadays considered to be beneficial to enhance the sensorial characteristics of the final products (taste, odor). however, microbial interactions occurring in such fermentations need to be better understood to improve the proc ... | 2014 | 24677041 |
| industrial robust yeast isolates with great potential for fermentation of lignocellulosic biomass. | the search of robust microorganisms is essential to design sustainable processes of second generation bioethanol. yeast strains isolated from industrial environments are generally recognised to present an increased stress tolerance but no specific information is available on their tolerance towards inhibitors that come from the pretreatment of lignocellulosic materials. in this work, a strategy for the selection of different yeasts using hydrothermal hydrolysate from eucalyptus globulus wood, co ... | 2014 | 24704884 |
| effect of oxygenation and temperature on glucose-xylose fermentation in kluyveromyces marxianus cbs712 strain. | the yeast kluyveromyces marxianus features specific traits that render it attractive for industrial applications. these include production of ethanol which, together with thermotolerance and the ability to grow with a high specific growth rate on a wide range of substrates, could make it an alternative to saccharomyces cerevisiae as an ethanol producer. however, its ability to co-ferment c5 and c6 sugars under oxygen-limited conditions is far from being fully characterized. | 2014 | 24712908 |
| the effect of some conditions on the secretion of extracellular beta-galactosidase synthesised by kluyveromyces fragilis 28. | to induce extracellular secretion of beta-galactosidase synthesised by kluyveromyces fragilis 28, it was used glycine, l-asparagine, l-leucine, dimethyl fluoride, dimethyl sulfoxide, cetyldimethylethylammonium bromide, penicillin g and glycolipids from candida antarctica. the highest increase in the secretion of beta-galactosidase was obtained in the yeast culture cultivated in the medium with polypeptone when glycine was used as the secretion inductor. the extracellular activity of beta-galacto ... | 2003 | 24757788 |
| a comparative study on galactoologosaccharide synthesis by selected beta-galactosidase preparations. | the transgalactosilating ability of beta-galactosidase preparations obtained from kluyveromyces fragilis, penicillium canescens, and escherichia coli was investigated. in samples of hydrolysates of 20% lactose solutions without or with a 2% addition of glucose, the galactose or fructose obtained with the use of the investigated preparations and the content of saccharides was determined with the hplc method. under experimental conditions, the highest quantity (56.0-64.1%) of galactooligosaccharid ... | 2003 | 24757791 |
| the characteristics of fermentation abilities of k. fragilis immobilized on siran. | the aim of this paper was to evaluate the suitability of siran, a highly porous mineral carrier, to immobilization of kluyveromyces fragilis--directly lactose-fermenting yeast. the ethanol concentration in the post-fermentation mash was 4.7-5.8% v/v and was less advantageous than in previous experiments with calcium alginate (on average 6.04% v/v in 9 sequential fermentations). the introduction of another yeast strain to the experiment--s. cerevisiae--enhanced the efficiency of the lactose mash ... | 2003 | 24757795 |
| a biosynthetic pathway for hexanoic acid production in kluyveromyces marxianus. | hexanoic acid can be used for diverse industrial applications and is a precursor for fine chemistry. although some natural microorganisms have been screened and evolved to produce hexanoic acid, the construction of an engineered biosynthetic pathway for producing hexanoic acid in yeast has not been reported. here we constructed hexanoic acid pathways in kluyveromyces marxianus by integrating 5 combinations of seven genes (atob, bktb, crt, hbd, mct1, ter, and tes1), by which random chromosomal si ... | 2014 | 24768798 |
| growth inhibition of thermotolerant yeast, kluyveromyces marxianus, in hydrolysates from cassava pulp. | in this study, we report the inhibition of kluyveromyces marxianus tistr5925 growth and ethanol fermentation in the presence of furan derivatives and weak acids (acetic acid and lactic acid) at high temperatures. cassava pulp, obtained as the waste from starch processing, was collected from 14 starch factories located in several provinces of thailand. at a high temperature (42 °c), the cassava pulp hydrolysate from some starch factories strongly inhibited growth and ethanol production of both k. ... | 2014 | 24781978 |
| development of a hybrid fermentation-enzymatic bioprocess for the production of ethyl lactate from dairy waste. | this work explores the potential for the development of a hybrid fermentation-enzymatic process for the production of ethyl lactate from dairy waste. cheese whey was used in kluyveromyces marxianus and lactobacillus bulgaricus batch cultures to produce ethanol and lactic acid respectively. subsequently, the fermentation products were transferred into an organic phase through liquid-liquid extraction and ethyl lactate was formed in an esterification reaction catalyzed by lipases. the production o ... | 2014 | 24785788 |
| perspectives for the biotechnological production of ethyl acetate by yeasts. | ethyl acetate is an environmentally friendly solvent with many industrial applications. the production of ethyl acetate currently proceeds by energy-intensive petrochemical processes which are based on natural gas and crude oil without exception. microbial synthesis of ethyl acetate could become an interesting alternative. the formation of esters as aroma compounds in food has been repeatedly reviewed, but a survey which deals with microbial synthesis of ethyl acetate as a bulk product is missin ... | 2014 | 24788328 |
| ethanol production from sunflower meal biomass by simultaneous saccharification and fermentation (ssf) with kluyveromyces marxianus atcc 36907. | the lignocellulosic materials are considered promising renewable resources for ethanol production, but improvements in the processes should be studied to reduce operating costs. thus, the appropriate enzyme loading for cellulose saccharification is critical for process economics. this study aimed at evaluating the concentration of cellulase and β-glucosidase in the production of bioethanol by simultaneous saccharification and fermentation (ssf) of sunflower meal biomass. the sunflower biomass wa ... | 2014 | 24794173 |
| whey fermentation by yeast strains kluyveromyces marxianus ucm y-2096 and ucm y-2388. | two kluyveromyces marxianus strains have been tested for their ability to ferment lactose in the medium containing cheese whey powder (cwp) and lactose at different concentrations. the ethanol production by the selected strains was inhibited by the increase in substrate concentration (100 g/l). the strain k. marxianus ucm y-2096 was found to yield the higher ethanol formation and to be more resistant to ethanol under microaerophilic conditions. | 2015 | 24800512 |
| mixed culture of kluyveromyces marxianus and candida krusei for single-cell protein production and organic load removal from whey. | the study was conducted to evaluate the potential of mixed culture of kluyveromyces marxianus and candida krusei to enhance cod removal efficiency, minimize contamination at extreme conditions (high temperature 40°c and low ph 3.5) during batch and continuous aerobic fermentation and to obtain improved quality single-cell protein (scp) using whey as substrate. the batch fermentation of mono-culture and mixed culture result showed that the mixed culture resulted in 8.8% higher cod removal efficac ... | 2014 | 24844166 |
| sulfuric acid hydrolysis and detoxification of red alga pterocladiella capillacea for bioethanol fermentation with thermotolerant yeast kluyveromyces marxianus. | one-step sulfuric acid saccharification of the red alga pterocladiella capillacea was optimized, and various detoxification methods (neutralization, overliming, and electrodialysis) of the acid hydrolysate were evaluated for fermentation with the thermotolerant yeast kluyveromyces marxianus. a proximate composition analysis indicated that p. capillacea was rich in carbohydrates. a significant galactose recovery of 81.1 ± 5% was also achieved under the conditions of a 12% (w/v) biomass load, 5% ( ... | 2014 | 24851812 |
| alcohol dehydrogenases from kluyveromyces marxianus: heterologous expression in escherichia coli and biochemical characterization. | kluyveromyces marxianus has recently become a species of interest for ethanol production since it can produce ethanol at high temperature and on a wide variety of substrates. however, the reason why this yeast can produce ethanol at high temperature is largely unknown. | 2014 | 24885162 |
| biosynthesis of 2-phenylethanol from glucose with genetically engineered kluyveromyces marxianus. | 2-phenylethanol (2-pe) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-pe is produced in a few yeast strains by ehrlich pathway. in this study, kluyveromyces marxianus was genetically engineered for overproduction of 2-pe from glucose. about 1.0g/l of 2-pe was produced by overexpressing phenylpyruvate decarboxylase (aro10) and alcohol dehydrogenase (adh2) genes of saccharomyces cerevisiae. a similar level of 2-pe was also produced from e ... | 2014 | 24910335 |
| production of folate in oat bran fermentation by yeasts isolated from barley and diverse foods. | the focus of the research was to identify yeasts from barley kernels in order to study their folate production capability while maintaining high viscosity caused by soluble fibres in oat bran fermentation. | 2014 | 24916316 |
| impact of microbial cultures on proteolysis and release of bioactive peptides in fermented milk. | this study aimed at evaluating co-cultures of selected microorganisms for their proteolytic activity and capability to produce fermented milk enriched with ace-inhibitory (acei) peptides. selected yeasts (torulaspora delbruekii kl66a, galactomyces geotrichum kl20b, pichia kudriavzevii kl84a and kluyveromyces marxianus kl26a) and lactic acid bacteria strains (lactobacillus plantarum lat03, lb. plantarum klat01 and the not virulent enterococcus faecalis ke06) were screened as single cultures for t ... | 2014 | 24929726 |
| deletion of a ku80 homolog enhances homologous recombination in the thermotolerant yeast kluyveromyces marxianus. | targeted gene replacement in the thermotolerant yeast kluyveromyces marxianus kctc 17555 has been hampered by its propensity to non-homologous end joining (nhej). to enhance homologous recombination (hr) by blocking nhej, we identified and disrupted the k. marxianus ku80 gene. the ku80 deletion mutant strain (kmku80∆) of k. marxianus kctc 17555 did not show apparent growth defects under several conditions with the exception of exposure to tunicamycin. the targeted disruption of the three model g ... | 2014 | 24930110 |
| uncommon opportunistic yeast bloodstream infections from qatar. | eleven uncommon yeast species that are associated with high mortality rates irrespective of antifungal therapy were isolated from 17/187 (201 episodes) pediatric and elderly patients with fungemia from qatar. the samples were taken over a 6-year period (january 2004-december 2010). isolated species included kluyveromyces marxianus, lodderomyces elongisporus, lindnera fabianii, candida dubliniensis, meyerozyma guilliermondii, candida intermedia, pichia kudriavzevii, yarrowia lipolytica, clavispor ... | 2014 | 24934803 |
| two missense mutations, e123q and k151e, identified in the erg11 allele of an azole-resistant isolate of candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia. | we report on the first cloning and nucleotide sequencing of an erg11 allele from a clinical isolate of candida kefyr cross-resistant to azole antifungals. it was recovered from a stem cell transplant patient, in an oncohematology unit exhibiting unexpected high prevalence of c. kefyr. two amino acid substitutions were identified: k151e, whose role in fluconazole resistance was already demonstrated in candida albicans, and e123q, a new substitution never described so far in azole-resistant candid ... | 2014 | 24936404 |
| fine structure of tibetan kefir grains and their yeast distribution, diversity, and shift. | tibetan kefir grains (tkgs), a kind of natural starter for fermented milk in tibet, china, host various microorganisms of lactic acid bacteria, yeasts, and occasionally acetic acid bacteria in a polysaccharide/protein matrix. in the present study, the fine structure of tkgs was studied to shed light on this unusual symbiosis with stereomicroscopy and thin sections. the results reveal that tkgs consist of numerous small grain units, which are characterized by a hollow globular structure with a di ... | 2014 | 24977409 |
| polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique. | accurate etiological diagnosis is the key to prevention of ocular morbidity in endophthalmitis cases. a 66 year old male was suffering from chronic endophthalmitis post-cataract surgery. polymerase chain reaction examination on anterior chamber fluid was positive for propionibacterium acnes but negative for the panfungal genome. he was advised vitrectomy with intravitreal injections. polymerase chain reaction of vitreous aspirate was positive for p.acnes as well as panfungal genome. the vitreous ... | 2014 | 25008833 |
| steam explosion treatment for ethanol production from branches pruned from pear trees by simultaneous saccharification and fermentation. | this study investigated the production of ethanol from unutilized branches pruned from pear trees by steam explosion pretreatment. steam pressures of 25, 35, and 45 atm were applied for 5 min, followed by enzymatic saccharification of the extracted residues with cellulase (cellic ctec2). high glucose recoveries, of 93.3, 99.7, and 87.1%, of the total sugar derived from the cellulose were obtained from water- and methanol-extracted residues after steam explosion at 25, 35, and 45 tm, respectively ... | 2014 | 25036499 |
| the predominance, biodiversity and biotechnological properties of kluyveromyces marxianus in the production of pecorino di farindola cheese. | pecorino di farindola is a handicraft cheese made by farmers on small scale using raw ewes' milk and pig rennet. in this study, yeast consortia were evaluated during pecorino di farindola making and ripening. molecular identification of 156 isolates was achieved by a combination of pcr-rflp of the 5.8s its rrna region and sequencing of the d1/d2 domain of the 26s rrna gene. kluyveromyces marxianus was the predominant species, while other species (pichia kudriavzevii, candida parapsilosis, candid ... | 2014 | 25038503 |
| draft genome sequence of kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate. | we determined the genome sequence of a thermotolerant yeast, kluyveromyces marxianus strain dmb1, isolated from sugarcane bagasse hydrolysate, and the sequence provides further insights into the genomic differences between this strain and other reported k. marxianus strains. the genome described here is composed of 11,165,408 bases and has 4,943 protein-coding genes. | 2014 | 25059876 |
| construction of recombinant kluyveromyces marxianus ufv-3 to express dengue virus type 1 nonstructural protein 1 (ns1). | the yeast kluyveromyces marxianus is a convenient host for industrial synthesis of biomolecules. however, despite its potential, there are few studies reporting the expression of heterologous proteins using this yeast. here, we report expression of a dengue virus protein in k. marxianus for the first time. the dengue virus type 1 nonstructural protein 1 (ns1) was integrated into the k. marxianus ufv-3 genome at the lac4 locus using an adapted integrative vector designed for high-level expression ... | 2015 | 25085614 |
| potential application of aqueous two-phase systems and three-phase partitioning for the recovery of superoxide dismutase from a clarified homogenate of kluyveromyces marxianus. | superoxide dismutase (sod; ec 1.15.1.1) is an antioxidant enzyme that represents the primary cellular defense against superoxide radicals and has interesting applications in the medical and cosmetic industries. in the present work, the partition behavior of sod in aqueous two-phase systems (atps) (using a standard solution and a complex extract from kluyveromyces marxianus as sample) was characterized on different types of atps (polymer-polymer, polymer-salt, alcohol-salt, and ionic liquid (il)- ... | 2016 | 25138773 |
| optimizing promoters and secretory signal sequences for producing ethanol from inulin by recombinant saccharomyces cerevisiae carrying kluyveromyces marxianus inulinase. | inulin is a polyfructan that is abundant in plants such as jerusalem artichoke, chicory and dahlia. inulinase can easily hydrolyze inulin to fructose, which is consumed by microorganisms. generally, saccharomyces cerevisiae, an industrial workhorse strain for bioethanol production, is known for not having inulinase activity. the inulinase gene from kluyveromyces marxianus (kminu), with the ability of converting inulin to fructose, was introduced into s. cerevisiae d452-2. the inulinase gene was ... | 2015 | 25142154 |
| analysis of the yeast short-term crabtree effect and its origin. | the short-term crabtree effect is defined as the immediate occurrence of aerobic alcoholic fermentation in response to provision of a pulse of excess sugar to sugar-limited yeast cultures. here we have characterized ten yeast species with a clearly defined phylogenetic relationship. yeast species were cultivated under glucose-limited conditions, and we studied their general carbon metabolism in response to a glucose pulse. we generated an extensive collection of data on glucose and oxygen consum ... | 2014 | 25161062 |
| screening of accurate clones for gene synthesis in yeast. | methods for error-less gene synthesis are desired because synthesized genes often contain mutations. by cloning pcr-assembled oligonucleotide fragments fused to a selection marker in yeast, we developed a novel method to screen accurate clones in gene synthesis. as a model case, the 555-bp luciferase gene from gaussia princeps (gluc) was synthesized to contain yeast-optimized codons (called ygluc hereafter). after standard pcr-mediated oligonucleotide assembly, many clones showed no luciferase a ... | 2015 | 25201012 |
| sustainable conversion of coffee and other crop wastes to biofuels and bioproducts using coupled biochemical and thermochemical processes in a multi-stage biorefinery concept. | the environmental impact of agricultural waste from the processing of food and feed crops is an increasing concern worldwide. concerted efforts are underway to develop sustainable practices for the disposal of residues from the processing of such crops as coffee, sugarcane, or corn. coffee is crucial to the economies of many countries because its cultivation, processing, trading, and marketing provide employment for millions of people. in coffee-producing countries, improved technology for treat ... | 2014 | 25204861 |
| opuntia ficus-indica cladodes as feedstock for ethanol production by kluyveromyces marxianus and saccharomyces cerevisiae. | the feasibility of ethanol production using an enzymatic hydrolysate of pretreated cladodes of opuntia ficus-indica (prickly pear cactus) as carbohydrate feedstock was investigated, including a comprehensive chemical analysis of the cladode biomass and the effects of limited aeration on the fermentation profiles and sugar utilization. the low xylose and negligible mannose content of the cladode biomass used in this study suggested that the hemicellulose structure of the o. ficus-indica cladode w ... | 2014 | 25248867 |
| [inulinases from various producers: the features of their permolecular organization]. | the structural organization of inulinases from yeasts, fungi, and plants are researched. for studying their sizes, molecular weight, and permolecular organization, an approach consisting of a combination of atomic force microscopy with methods of dynamic light scattering, gel chromatography, and electrophoresis was used. it is shown that inulinases from kluyveromyces marxianus and aspergillus niger form geterodimers and inulinases from tubers of helianthus tuberosus are present as both dimers an ... | 2014 | 25272747 |
| growth and by-product profiles of kluyveromyces marxianus cells immobilized in foamed alginate. | the aim of this research was to study how the yeast cell immobilization technique influences the growth and fermentation profiles of kluyveromyces marxianus cultivated on apple/chokeberry and apple/cranberry pomaces. encapsulation of the cells was performed by droplet formation from a foamed alginate solution. the growth and metabolic profiles were evaluated for both free and immobilized cells. culture media with fruit waste produced good growth of free as well as immobilized yeast cells. the fe ... | 2015 | 25277269 |
| influence of carbon and nitrogen source on production of volatile fragrance and flavour metabolites by the yeast kluyveromyces marxianus. | the yeast kluyveromyces marxianus produces a range of volatile molecules with applications as fragrances or flavours. the purpose of this study was to establish how nutritional conditions influence the production of these metabolites. four strains were grown on synthetic media, using a variety of carbon and nitrogen sources and volatile metabolites analysed using gas chromatography-mass spectrometry (gc-ms). the nitrogen source had pronounced effects on metabolite production: levels of the fusel ... | 2015 | 25308412 |
| protein enrichment of an opuntia ficus-indica cladode hydrolysate by cultivation of candida utilis and kluyveromyces marxianus. | the cladodes of opuntia ficus-indica (prickly pear cactus) have a low protein content; for use as a balanced feed, supplementation with other protein sources is therefore desirable. we investigated protein enrichment by cultivation of the yeasts candida utilis and kluyveromyces marxianus in an enzymatic hydrolysate of the cladode biomass. | 2015 | 25371280 |
| genomic sequence of the yeast kluyveromyces marxianus cct 7735 (ufv-3), a highly lactose-fermenting yeast isolated from the brazilian dairy industry. | here, we present the draft genome sequence of kluyveromyces marxianus cct 7735 (ufv-3), including the eight chromosomes and the mitochondrial genomic sequences. | 2014 | 25377712 |
| cell factory applications of the yeast kluyveromyces marxianus for the biotechnological production of natural flavour and fragrance molecules. | kluyveromyces marxianus is emerging as a new platform organism for the production of flavour and fragrance (f&f) compounds. this food-grade yeast has advantageous traits, such as thermotolerance and rapid growth, that make it attractive for cell factory applications. the major impediment to its development has been limited fundamental knowledge of its genetics and physiology, but this is rapidly changing. k. marxianus produces a wide array of volatile molecules and contributes to the flavour of ... | 2015 | 25393382 |
| cloning and characterization of an inulinase gene from the marine yeast candida membranifaciens subsp. flavinogenie w14-3 and its expression in saccharomyces sp. w0 for ethanol production. | the inu1 gene encoding an exo-inulinase from the marine-derived yeast candida membranifaciens subsp. flavinogenie w14-3 was cloned and characterized. it had an open reading frame of 1,536 bp long encoding an inulinase. the coding region of it was not interrupted by any intron. the cloned gene encoded 512 amino acid residues of a protein with a putative signal peptide of 23 amino acids and a calculated molecular mass of 57.8 kda. the protein sequence deduced from the inulinase gene contained the ... | 2015 | 25432793 |
| isotope labeling pattern study of central carbon metabolites using gc/ms. | determination of fluxes by (13)c tracer experiments depends on monitoring the (13)c labeling pattern of metabolites during isotope experiments. in metabolome-based (13)c metabolic flux analysis, liquid chromatography combined with mass spectrometry or tandem mass spectrometry (lc/ms or lc/ms/ms, respectively) has been mainly used as an analytical platform for isotope pattern studies of central carbon metabolites. however, gas chromatography with mass spectrometry (gc/ms) has several advantages o ... | 2015 | 25463204 |
| improving xylitol production at elevated temperature with engineered kluyveromyces marxianus through over-expressing transporters. | three transporter genes including kluyveromyces marxianus aquaglyceroporin gene (kmfps1), candida intermedia glucose/xylose facilitator gene (cigxf1) or glucose/xylose symporter gene (cigxs1) were over-expressed in k. marxianus yzj017 to improve xylitol production at elevated temperatures. the xylitol production of yzj074 that harbored cigxf1 was improved to 147.62g/l in erlenmeyer flask at 42°c. in fermenter, 99.29 and 149.60g/l xylitol were produced from 99.55 and 151.91g/l xylose with product ... | 2015 | 25465792 |
| whole cells in enantioselective reduction of benzyl acetoacetate. | the β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (s)-3-hydroxybutanoate by seven microorganism species. the best result using free cells was obtained with the yeast hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. after immobilization in calcium alginate spheres, k.marxianus showed to be more stable after 2 cycles of reaction. | 2014 | 25477927 |
| studies on the mechanism of synthesis of ethyl acetate in kluyveromyces marxianus dsm 5422. | kluyveromyces marxianus converts whey-borne sugar into ethyl acetate, an environmentally friendly solvent with many applications. k. marxianus dsm 5422 presumably synthesizes ethyl acetate from acetyl-scoa. iron limitation as a trigger for this synthesis is explained by a diminished aconitase and succinate dehydrogenase activity (both enzymes depend on iron) causing diversion of acetyl-scoa from the tricarboxic acid cycle to ester synthesis. copper limitation as another trigger for ester synthes ... | 2015 | 25487884 |
| enhanced enzymatic hydrolysis and ethanol production from cashew apple bagasse pretreated with alkaline hydrogen peroxide. | the effect of combinations and ratios between different enzymes has been investigated in order to assess the optimal conditions for hydrolysis of cashew apple bagasse pretreated with alkaline hydrogen peroxide (the solids named cab-ahp). the separate hydrolysis and fermentation (shf) and simultaneous saccharification and fermentation (ssf) processes were evaluated in the ethanol production. the enzymatic hydrolysis conducted with cellulase complex and β-glucosidase in a ratio of 0.61:0.39, enzym ... | 2015 | 25545094 |
| bioflavour production from tomato and pepper pomaces by kluyveromyces marxianus and debaryomyces hansenii. | bioflavours are called natural flavour and/or fragrance compounds which are produced using metabolic pathway of the microorganism and/or plant cells or their enzyme systems with bioengineering approaches. the aim of this study was to investigate bioflavour production from tomato and red pepper pomaces by kluyveromyces marxianus and debaryomyces hansenii. obtained specific growth rates of k. marxianus and d. hansenii in tomato pomace were 0.081/h and 0.177/h, respectively. the bioflavour profile ... | 2015 | 25614449 |
| [investigation of mechanisms of interaction between inulinase from kluyveromyces marxianus and the matrices of ion-exchange resins and fiber]. | it is established that ion exchange resins av-17-2p, ku-2, av-16-gs, am 21a, imac-hp, purolite and fiber vion kn-1 can be applied as carriers for inulinase immobilization. the analysis of ir spectra for an enzyme, carriers and heterogeneous enzyme preparations showed that inulinase binding to matrices of various carriers occurs in general through electrostatic interactions. it is assumed that the mechanisms of interaction between inulinase from kluyveromyces marxianus and the matrices of cation- ... | 2015 | 25702479 |
| comparison of a pectinolytic extract of kluyveromyces marxianus and a commercial enzyme preparation in the production of ives (vitis labrusca) grape juice. | this study analyses the effect of the crude enzymatic extract produced by kluyveromyces marxianus (eeb) in the maceration and clarification of juice produced from ives (vitis labrusca) grapes compared to the commercial enzyme preparation pectinex(®)ultra color (pec). treatments were conducted with a total pectinolytic activity of 1 u/ml of fruit juice, at 40 °c, for 60 min. after the enzymatic treatment, the juices were evaluated with respect to yield, viscosity, and degree of clarification, as ... | 2015 | 25721421 |
| short communication: evaluation of the microbiota of kefir samples using metagenetic analysis targeting the 16s and 26s ribosomal dna fragments. | milk kefir is produced by fermenting milk in the presence of kefir grains. this beverage has several benefits for human health. the aim of this experiment was to analyze 5 kefir grains (and their products) using a targeted metagenetic approach. of the 5 kefir grains analyzed, 1 was purchased in a supermarket, 2 were provided by the ministry of agriculture (namur, belgium), and 2 were provided by individuals. the metagenetic approach targeted the v1-v3 fragment of the 16s ribosomal (r)dna for the ... | 2015 | 25828663 |
| genetic basis of the highly efficient yeast kluyveromyces marxianus: complete genome sequence and transcriptome analyses. | high-temperature fermentation technology with thermotolerant microbes has been expected to reduce the cost of bioconversion of cellulosic biomass to fuels or chemicals. thermotolerant kluyveromyces marxianus possesses intrinsic abilities to ferment and assimilate a wide variety of substrates including xylose and to efficiently produce proteins. these capabilities have been found to exceed those of the traditional ethanol producer saccharomyces cerevisiae or lignocellulose-bioconvertible ethanolo ... | 2015 | 25834639 |
| characterization of the kluyveromyces marxianus strain dmb1 ygl157w gene product as a broad specificity nadph-dependent aldehyde reductase. | the open reading frame ygl157w in the genome of the yeast kluyveromyces marxianus strain dmb1 encodes a putative uncharacterized oxidoreductase. however, this protein shows 46% identity with the saccharomyces cerevisiae s288c nadph-dependent methylglyoxal reductase, which exhibits broad substrate specificity for aldehydes. in the present study, the ygl157w gene product (kmgre2) was purified to homogeneity from overexpressing escherichia coli cells and found to be a monomer. the enzyme was strict ... | 2015 | 25852994 |
| genetic diversity of medically important and emerging candida species causing invasive infection. | genetic variation in the ribosomal dna (rdna) internal transcribed spacer (its) region has been studied among fungi. however, the numbers of its sequence polymorphisms in the various candida species and their associations with sources of invasive fungal infections remain poorly investigated. here, we characterized the intraspecific and interspecific its diversity of candida spp. strains collected from patients with bloodstream or oroesophageal candidiasis. | 2015 | 25887032 |
| the effect of lactic acid bacteria on cocoa bean fermentation. | cocoa beans (theobroma cacao l.) are the raw material for chocolate production. fermentation of cocoa pulp by microorganisms is crucial for developing chocolate flavor precursors. yeasts conduct an alcoholic fermentation within the bean pulp that is essential for the production of good quality beans, giving typical chocolate characters. however, the roles of bacteria such as lactic acid bacteria and acetic acid bacteria in contributing to the quality of cocoa bean and chocolate are not fully und ... | 2015 | 25889523 |
| synthetic signal sequences that enable efficient secretory protein production in the yeast kluyveromyces marxianus. | targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the n-terminus of a secretory protein. these signal sequences usually contain an n-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signal sequence has been identified. in this study, simple modeling of signal sequences was attempted using gaussia princeps secretory luciferase (gluc) in the yeast kluyveromyces marxianus, which ... | 2015 | 25889890 |
| complete genome sequence of kluyveromyces marxianus nbrc1777, a nonconventional thermotolerant yeast. | we determined the genome sequence of the thermotolerant yeast kluyveromyces marxianus strain nbrc1777. the genome of strain nbrc1777 is composed of 4,912 open reading frames (orfs) on 8 chromosomes, with a total size of 10,895,581 bp, including mitochondrial dna. | 2015 | 25908152 |
| phytase-producing capacity of yeasts isolated from traditional african fermented food products and phypk gene expression of pichia kudriavzevii strains. | phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. ninety-three yeast isolates from traditional african fermented food products, belonging to nine species (pichia kudriavzevii, saccharomyces cerevisiae, clavispora lusitaniae, kluyveromyces marxianus, millerozyma farinosa, candida glabrata, wickerhamomyces anomalus, hanseniaspora guilliermondii and debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were a ... | 2015 | 25910031 |
| [expression of inulinase genes in the yeasts saccharomyces cerevisiae and kluyveromyces marxianus]. | expression of the genes encoding the enzymes involved in inulin, sucrose, and glucose metabolism in the yeasts saccharomyces cerevisiae and kluyveromyces marxianus was studied. the exon-intron structure of the relevant genes was identified and the primers for quantitative pcr were optimized. expression of the genes was found to depend on the carbon source. glucose was shown to exhibit a repressive effect on inulinase synthesis by k. marxianus, while in s. cerevisiae glucose and sucrose were inul ... | 2015 | 25916145 |
| feasibility of biohydrogen production from industrial wastes using defined microbial co-culture. | the development of clean or novel alternative energy has become a global trend that will shape the future of energy. in the present study, 3 microbial strains with different oxygen requirements, including clostridium acetobutylicum atcc 824, enterobacter cloacae atcc 13047 and kluyveromyces marxianus 15d, were used to construct a hydrogen production system that was composed of a mixed aerobic-facultative anaerobic-anaerobic consortium. the effects of metal ions, organic acids and carbohydrate su ... | 2015 | 25943991 |
| transgalactosylation of lactose for synthesis of galacto-oligosaccharides using kluyveromyces marxianus ncim 3551. | among a number of yeast strains screened for whole cell transgalactosylating activity, kluyveromyces marxianus ncim 3551 was found to be most suitable biocatalyst for production of galacto-oligosaccharides (gos). cell permeabilization lead to an efficient bioconversion by β-galactosidase resulting in synthesis of gos. a maximum gos yield of 36% (w/w) of total sugars was achieved and the products consisted of tri- and tetra-galacto-oligosaccharides. a lactose conversion rate of 80% and productivi ... | 2015 | 25976627 |
| ethanol production from jerusalem artichoke tubers at high temperature by newly isolated thermotolerant inulin-utilizing yeast kluyveromyces marxianus using consolidated bioprocessing. | thermotolerant inulin-utilizing yeast strains were successfully isolated in this study. among the isolated strains, kluyveromyces marxianus dbkku y-102 was found to be the most effective strain for direct ethanol fermentation at high temperature from fresh jerusalem artichoke (ja) tubers without inulin hydrolysis under consolidated bioprocessing (cbp). the maximum ethanol concentrations produced by this strain under the optimum culture conditions were 104.83 and 97.46 g l(-1) at 37 and 40 °c, re ... | 2015 | 25980834 |
| microbial biogeography of the transnational fermented milk matsoni. | the fermented milk matsoni is a traditional, national food product of both georgia and armenia. little is known about the effects of biogeography and milk type on the microbial biodiversity of matsoni or the fungal composition of matsoni fermentations. high-throughput marker-gene sequencing was used to survey the bacterial and fungal communities of matsoni from different milk types and regions throughout armenia and georgia. results demonstrate that both production region and milk type influence ... | 2015 | 25998810 |
| production of lactulose oligosaccharides by isomerisation of transgalactosylated cheese whey permeate obtained by β-galactosidases from dairy kluyveromyces. | β-galactosidases from kluyveromyces lactis and kluyveromyces marxianus isolated from artisanal ewes' milk cheeses, were used to transgalactosylate lactose from cheese whey permeate (wp). the content of galactooligosaccharides (gos) obtained by transgalactosylation was comparable with that formed using pure lactose as substrate. in order to obtain a mixture with higher prebiotic oligosaccharide content, isomerisation of the transgalactosylated wp was carried out using sodium aluminate as catalyst ... | 2015 | 26004434 |
| neuro-fuzzy based model of batch fermentation of kluyveromyces marxianus var. lactis mc5. | in this work a neuro-fuzzy based model of a whey batch fermentation process by a strain kluyveromyces marxianus var. lactis mc5 is presented. a three-layered neuro-fuzzy network is realized. the simulation results are compared with conventional models (based on mass balance and differential equations). the neuro-fuzzy model provides a better fitness and allows inclusion of linguistic variables (such as colour, smell, taste, morphophysiology, etc.). the accuracy is approximately equal to this ach ... | 2014 | 26019585 |
| lipid production from hemicellulose and holocellulose hydrolysate of palm empty fruit bunches by newly isolated oleaginous yeasts. | palm empty fruit bunches (efbs) are abundant lignocellulosic wastes from palm oil mills. they are potential sources of sugars which can be converted to microbial lipids by oleaginous yeasts. to produce sugars from efb, two-step and one-step hydrolysis reactions were performed. in the first step, the use of diluted sulfuric acid (0.5 % w/v) hydrolyzed hemicelluloses and released mainly pentoses, and in the second step of hydrolysis of residual pulp using 2.5 % (w/v), sulfuric acid released more h ... | 2015 | 26026262 |
| enhanced xylitol production by mutant kluyveromyces marxianus 36907-fmel1 due to improved xylose reductase activity. | a directed evolution and random mutagenesis were carried out with thermotolerant yeast kluyveromyces marxianus atcc 36907 for efficient xylitol production. the final selected strain, k. marxianus 36907-fmel1, exhibited 120 and 39 % improvements of xylitol concentration and xylitol yield, respectively, as compared to the parental strain, k. marxianus atcc 36907. according to enzymatic assays for xylose reductase (xr) activities, xr activity from k. marxianus 36907-fmel1 was around twofold higher ... | 2015 | 26043853 |
| cell lysis induced by membrane-damaging detergent saponins from quillaja saponaria. | this paper presents the results of a study to determine the effect of quillaja saponaria saponins on the lysis of industrial yeast strains. cell lysis induced by saponin from q. saponaria combined with the plasmolysing effect of 5% nacl for saccharomyces cerevisiae, kluyveromyces marxianus yeasts biomass was conducted at 50 °c for 24-48 h. membrane permeability and integrity of the yeast cells were monitored using fluorescent techniques and concentrations of proteins, free amino nitrogen (fan) a ... | 2015 | 26047915 |
| looking beyond saccharomyces: the potential of non-conventional yeast species for desirable traits in bioethanol fermentation. | saccharomyces cerevisiae has been used for millennia in the production of food and beverages and is by far the most studied yeast species. currently, it is also the most used microorganism in the production of first-generation bioethanol from sugar or starch crops. second-generation bioethanol, on the other hand, is produced from lignocellulosic feedstocks that are pretreated and hydrolyzed to obtain monomeric sugars, mainly d-glucose, d-xylose and l-arabinose. recently, s. cerevisiae recombinan ... | 2015 | 26126524 |
| novel transporters from kluyveromyces marxianus and pichia guilliermondii expressed in saccharomyces cerevisiae enable growth on l-arabinose and d-xylose. | genes encoding l-arabinose transporters in kluyveromyces marxianus and pichia guilliermondii were identified by functional complementation of saccharomyces cerevisiae whose growth on l-arabinose was dependent on a functioning l-arabinose transporter, or by screening a differential display library, respectively. these transporters also transport d-xylose and were designated kmaxt1 (arabinose-xylose transporter) and pgaxt1, respectively. transport assays using l-arabinose showed that kmaxt1p has k ... | 2015 | 26129747 |
| characterization of osmotolerant yeasts and yeast-like molds from apple orchards and apple juice processing plants in china and investigation of their spoilage potential. | yeasts and yeast-like fungal isolates were recovered from apple orchards and apple juice processing plants located in the shaanxi province of china. the strains were evaluated for osmotolerance by growing them in 50% (w/v) glucose. of the strains tested, 66 were positive for osmotolerance and were subsequently identified by 26s or 5.8s-its ribosomal rna (rrna) gene sequencing. physiological tests and rapd-pcr analysis were performed to reveal the polymorphism of isolates belonging to the same sp ... | 2015 | 26130165 |
| gene expression analysis using strains constructed by nhej-mediated one-step promoter cloning in the yeast kluyveromyces marxianus. | gene expression analysis provides valuable information to evaluate cellular state. unlike quantitative mrna analysis techniques like reverse-transcription pcr and microarray, expression analysis using a reporter gene has not been commonly used for multiple-gene analysis, probably due to the difficulty in preparing multiple reporter-gene constructs. to circumvent this problem, we developed a novel one-step reporter-gene construction system mediated by non-homologous end joining (nhej) in the yeas ... | 2015 | 26136515 |
| selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting. | the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ... | 2014 | 26139877 |
| characterizing yeast promoters used in kluyveromyces marxianus. | fermentation at higher temperatures can potentially reduce the cooling cost in large-scale fermentation and reduce the contamination risk. thus, the thermotolerant yeast, kluyveromyces marxianus, which can grow and ferment at elevated temperatures, is a promising biotechnological tool for future applications. however, the promoters used in k. marxianus are not well characterized, especially at elevated temperatures, which is important in efficient metabolic pathway construction. in this study, s ... | 2015 | 26164057 |
| molecular cloning and characterization of two ygl039w genes encoding broad specificity nadph-dependent aldehyde reductases from kluyveromyces marxianus strain dmb1. | two genes from kluyveromyces marxianus strain dmb1, ygl039w1 and ygl039w2, encode putative uncharacterized oxidoreductases that respectively share 42 and 44% identity with the saccharomyces cerevisiae s288c nadph-dependent methylglyoxal reductase (ec 1.1.1.283). to determine the enzymatic characteristics of their products, the two genes were expressed in recombinant escherichia coli cells, after which the ygl039w1 and ygl039w2 proteins were purified to homogeneity. in the presence of nadph, both ... | 2015 | 26223585 |
| the modeling of ethanol production by kluyveromyces marxianus using whey as substrate in continuous a-stat bioreactors. | we investigated the kinetics of whey bioconversion into ethanol by kluyveromyces marxianus in continuous bioreactors using the "accelerostat technique" (a-stat). cultivations using free and ca-alginate immobilized cells were evaluated using two different acceleration rates (a). the kinetic profiles of these systems were modeled using four different unstructured models, differing in the expressions for the specific growth (μ) and substrate consumption rates (r s), taking into account substrate li ... | 2015 | 26233317 |
| rapid ethanol production at elevated temperatures by engineered thermotolerant kluyveromyces marxianus via the nadp(h)-preferring xylose reductase-xylitol dehydrogenase pathway. | conversion of xylose to ethanol by yeasts is a challenge because of the redox imbalances under oxygen-limited conditions. the thermotolerant yeast kluyveromyces marxianus grows well with xylose as a carbon source at elevated temperatures, but its xylose fermentation ability is weak. in this study, a combination of the nadph-preferring xylose reductase (xr) from neurospora crassa and the nadp(+)-preferring xylitol dehydrogenase (xdh) mutant from scheffersomyces stipitis (pichia stipitis) was cons ... | 2015 | 26253204 |
| yeast kluyveromyces lactis as host for expression of the bacterial lipase: cloning and adaptation of the new lipase gene from serratia sp. | many microbial lipases have been successfully expressed in yeasts, but not in industrially attractive kluyveromyces lactis, which among other benefits can be cultivated on a medium supplemented with whey--cheap and easily available industrial waste. a new bacterial lipase from serratia sp. was isolated and for the first time expressed into the yeast kluyveromyces lactis by heterologous protein expression system based on a strong promoter of kluyveromyces marxianus triosephosphate isomerase gene ... | 2015 | 26254038 |
| hypocholesterolemic effects of kluyveromyces marxianus m3 isolated from tibetan mushrooms on diet-induced hypercholesterolemia in rat. | to investigate the effects of kluyveromyces marxianus m3 isolated from tibetan mushrooms on diet-induced hypercholesterolemia in rats, female wistar rats were fed a high-cholesterol diet (hcd) for 28 d to generate hyperlipidemic models. hyperlipidemic rats were assigned to four groups, which were individually treated with three different dosages of k. marxianus m3+hcd or physiological saline+hcd via oral gavage for 28 d. the total cholesterol (tc), triglycerides (tg), high-density lipoprotein ch ... | 2015 | 26273253 |
| transcriptional analysis of kluyveromyces marxianus for ethanol production from inulin using consolidated bioprocessing technology. | ethanol production from non-crop materials, such as jerusalem artichokes, would make a great contribution to the energy industry. the non-conventional yeast, kluyveromyces marxianus, is able to carry out ethanol fermentation of sugar molecules obtained from inulin-containing materials by consolidated bioprocessing. lower inulin concentrations and micro-aeration can lead to a relatively fast and ideal fermentation process; however, it is unclear what causes the inhibition of higher concentrations ... | 2015 | 26273319 |
| non-saccharomyces yeasts protect against epithelial cell barrier disruption induced by salmonella enterica subsp. enterica serovar typhimurium. | the human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. whereas invasive pathogens cause epithelial barrier disruption, probiotic micro-organisms modulate tight junction regulation and improve epithelial barrier function. in addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. the aim of this study was to explore non-saccharomyces yeast modulation of epithelial cell barrier func ... | 2015 | 26280244 |
| chromosome arrangement, differentiation of growth kinetics and volatile molecule profiles in kluyveromyces marxianus strains from italian cheeses. | thirty-nine strains of kluyveromyces marxianus from pecorino di farindola cheese in comparison with 3 strains from parmigiano reggiano cheese, 1 from fermented milk, 3 from cow whey and two type strains k. marxianus cbs 834(t) and kluyveromyces lactis cbs 683(t) were tested for genetic and metabolic characteristics. intraspecific diversity of chromosome arrangements was evaluated by pulsed field gel electrophoresis (pfge) analysis. among k. marxianus strains chromosome polymorphisms were evident ... | 2015 | 26310129 |
| yeast diversity in a traditional french cheese "tomme d'orchies" reveals infrequent and frequent species with associated benefits. | this study is aimed at unrevealing the yeast diversity of handmade cheese, tomme d'orchies, produced and marketed in the north of france. a total of 185 yeast colonies were isolated from the surface and core of this cheese. from these, 80 morphologically different colonies were selected and subjected to rep-pcr analysis. the isolates were clustered into six distinct groups based on their dna fingerprints. from each group, at least 30% of isolates were selected and identified to species level by ... | 2015 | 26338133 |
| coexistence of lactic acid bacteria and potential spoilage microbiota in a dairy processing environment. | microbial contamination in food processing plants can play a fundamental role in food quality and safety. in this study, the microbiota in a dairy plant was studied by both 16s rrna- and 26s rrna-based culture-independent high-throughput amplicon sequencing. environmental samples from surfaces and tools were studied along with the different types of cheese produced in the same plant. the microbiota of environmental swabs was very complex, including more than 200 operational taxonomic units with ... | 2015 | 26341209 |
| a new search for thermotolerant yeasts, its characterization and optimization using response surface methodology for ethanol production. | the progressive rise in energy crisis followed by green house gas (ghg) emissions is serving as the driving force for bioethanol production from renewable resources. current bioethanol research focuses on lignocellulosic feedstocks as these are abundantly available, renewable, sustainable and exhibit no competition between the crops for food and fuel. however, the technologies in use have some drawbacks including incapability of pentose fermentation, reduced tolerance to products formed, costly ... | 2015 | 26388844 |
| thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production. | nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at brazilian cerrado and identified up to species level on the basis of the gene sequencing of 5.8s-its and 26s ribosomal dna regions. four species were identified: kluyveromyces marxianus, aspergillus niger, aspergillus sydowii and aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. among them, th ... | 2017 | 26413077 |
| the thermotolerant yeast kluyveromyces marxianus is a useful organism for structural and biochemical studies of autophagy. | autophagy is a conserved degradation process in which autophagosomes are generated by cooperative actions of multiple autophagy-related (atg) proteins. previous studies using the model yeast saccharomyces cerevisiae have provided various insights into the molecular basis of autophagy; however, because of the modest stability of several atg proteins, structural and biochemical studies have been limited to a subset of atg proteins, preventing us from understanding how multiple atg proteins functio ... | 2015 | 26442587 |
| cloning, expression, characterization, and mutagenesis of a thermostable exoinulinase from kluyveromyces cicerisporus. | inulinase is an enzyme that belongs to glycoside hydrolase family 32. it converts inulin into high-fructose syrups and fructoligosaccharides, both of which are widely used in pharmaceutical and food industries. in this study, the kcinu1 gene (genbank accession number af178979) encoding an exoinulinase was cloned from kluyveromyces cicerisporus cbs4857 and expressed in pichia pastoris x-33, yielding a maximum of 45.2 ± 0.6 u ml(-1) of inulinase activity of culture supernatant. the expressed inuli ... | 2016 | 26446826 |
| kefir-isolated bacteria and yeasts inhibit shigella flexneri invasion and modulate pro-inflammatory response on intestinal epithelial cells. | the aim of this work was to evaluate the ability of a kefir-isolated microbial mixture containing three bacterial and two yeast strains (mm) to protect intestinal epithelial cells against shigella flexneri invasion, as well as to analyse the effect on pro-inflammatory response elicited by this pathogen. a significant decrease in s. flexneri strain 72 invasion was observed on both ht-29 and caco-2 cells pre-incubated with mm. pre-incubation with the individual strains saccharomyces cerevisiae cid ... | 2016 | 26503738 |
| dynamics of yeast immobilized-cell fluidized-bed bioreactors systems in ethanol fermentation from lactose-hydrolyzed whey and whey permeate. | we studied the dynamics of ethanol production on lactose-hydrolyzed whey (lhw) and lactose-hydrolyzed whey permeate (lhwp) in batch fluidized-bed bioreactors using single and co-cultures of immobilized cells of industrial strains of saccharomyces cerevisiae and non-industrial strains of kluyveromyces marxianus. although the co-culture of s. cerevisiae cat-1 and k. marxianus cct 4086 produced two- to fourfold the ethanol productivity of single cultures of s. cerevisiae, the single cultures of the ... | 2016 | 26527573 |