Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| human neutrophils endocytose multivalent ligands from the surface of schistosomula of schistosoma mansoni before membrane fusion. | human buffy coat cells adhering to schistosomula of schistosoma mansoni that were preincubated in fluorochrome-conjugated concanavalin a (con a), wheat germ agglutinin, lentil lectin, or purified igg from a hyperimmunized rabbit, were examined by fluorescence and transmission electron microscopy and by freeze-fracture. all four fluorochrome-conjugated multivalent ligands were homogeneously distributed on the parasite surface after preincubation. within 1-3 h after the addition of cells, large ar ... | 1982 | 7107703 |
| comparative analysis of procedures used to isolate variant antigen from trypanosoma brucei rhodesiense. | comparisons made among various procedures leading to the isolation of variant antigen from trypanosoma brucei rhodesiense bloodstream trypomastigotes. as a means of parasite disruption, freeze-thawing solubilized 36% more variant antigen than did sonication. protease inhibitors were important additions to the suspension prior to cellular disruption. if trypanosomal extracts were frozen for at least 1 wk prior to chromatographic isolation of variant antigen, recovery of the antigen was reduced by ... | 1982 | 7119984 |
| non-covalent interactions result in aggregation of surface antigens of the parasitic nematode trichinella spiralis. | surface antigens of three stages of the nematode worm trichinella spiralis has been labelled with iodine and examined by sodium dodecyl sulphate (sds)/polyacrylamide-gel electrophoresis under reducing and non-reducing conditions. a variety of interactions were defined: the infective larva surface antigens formed a spectrum of aggregates from 50kda to greater than 1000kda from subunits of 47kda and 90kda; in the adult worms of 60kda complex arose fron interaction between two dissimilar molecules ... | 1982 | 7126193 |
| tryptic peptide map analysis of the major human blood platelet membrane glycoproteins separated by two-dimensional polyacrylamide gel electrophoresis. | washed platelets were surface-labelled by lactoperoxidase catalyzed iodination and either the platelets or membranes were solubilized in detergent and applied to a wheat germ agglutinin-sepharose column and a lens culinaris lectin sepharose column coupled sequentially. the glycoproteins eluted from the lectin columns were separated by two-dimensional gel electrophoresis. alternatively, labelled whole platelets or membranes were solubilized and then directly separated by two-dimensional polyacry ... | 1982 | 7126562 |
| use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa. | paraffin-embedded human bronchial biopsies, obtained in macroscopically healthy areas, were examined using nine peroxidase-bound lectins. these were either isolated or purified by affinity column chromatography (ulex europeus, triticum vulgare, glycine max, and arachis hypogatea lectins) or commercial preparations (lotus tetragonolobus, canavalia ensiformis, helix pomatia, phaseolus vulgaris, and lens culinaris lectins) and conjugated to peroxidase (except for concanavalin a). these labeled lect ... | 1982 | 7130674 |
| similarity of acetylcholine receptors of denervated, innervated and embryonic chicken muscles. 1. molecular species and their purification. | acetylcholine receptors were purified to homogeneity from chicken embryonic, adult innervated and denervated muscles, by bio-specific chromatographies using immobilised alpha-neurotoxin and lentil lectin. a minimum specific activity for the pure receptor was estimated to be 6000 nmol alpha-toxin binding sites/g protein. for analysis, the receptors were radio-iodinated or tritiated to high specific radioactivity with succinimidyl-[2,3-3h]propionate. all of the iodinated protein present in the pur ... | 1982 | 7140738 |
| circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols. | the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ... | 1982 | 7150429 |
| cell kinetics and radiosensitivity of human lymphocytes stimulated by phytohemagglutinin, wistaria floribunda or lentil lectin. | the cell kinetics and the radiosensitivities of human lymphocytes (four donors) exposed to 200 rads of x-rays and stimulated with phytohemagglutinin (pha), wistaria floribunda (wfa) or lens culinaris (lch-a) extracts have been compared after cultivation times from 42 up to 54 h. all these mitogens are considered activating primarily t lymphocytes. pha is a tetrameric molecule, whereas wfa as well as lch-a are dimeric structures having only two reactive sites. pha displays a higher mitogenic acti ... | 1982 | 7182059 |
| volatile and gaseous metabolites released by germinating seeds of lentil and maize cultivars with different susceptibilities to fusariosis and smut. | the effect of volatile and gaseous metabolites released by germinating seeds of lentil cultivars more and less susceptible to fusariosis on the germination of spores of mucor racemosus, trichoderma viride, verticillium dahliae and botrytis cinerea was found to depend rather on the fungal genus than on the lentil cultivar. however, spores of fusarium oxysporum reacted more sensitively during germination to the presence of exudates of both cultivars, when the more susceptible lentil displayed a st ... | 1980 | 7189738 |
| dimethyl sulfoxide inhibits capping of surface receptors. | the microfilaments of cellular slime mold cells are known to be dislocated from their attachment to the plasma membrane by 10% dimethyl sulfoxide (dmso). we report here that 10% dmso inhibited capping of lentil lectin receptors on the surface of vegetative cells of the cellular slime mold, dictyostelium mucoroides. when the dmso was washed from the cells, capping took place. capping of surface immunoglobulin of mouse lymphocytes was also reversibly inhibited by 10% dmso. | 1981 | 7195781 |
| glycoproteins of platelet membranes from glanzmann's thrombasthenia. a comparison with normal using carbohydrate-specific or protein-specific labelling techniques and high-resolution two-dimensional gel electrophoresis. | platelets from glanzmann's thrombasthenia patients and from normal donor were surface labeled by techniques specific for sugars (terminal sialic acid, penultimate galactose/n-acetylgalactosamine) and proteins (tyrosine-histidine residues). these labelled platelets were solubilized in sodium dodecyl sulphate and separated on a two-dimensional electrophoretic system [o'farrell. p. h. (1975) j. biol. chem. 250, 4007--4021] first according to their isoelectric point (pi) and then according to their ... | 1981 | 7195813 |
| structural studies of purified pig lymph node plasma membrane: association of cytoskeletal compounds with the plasma membrane and the effect of detergent solubilization. | the reproducibility of preparation, stability at 4 degrees c, and detergent solubilization characteristics of plasma membrane vesicles purified from domestic pig mesenteric lymph node tissue have been examined. it was found that 2% (w/v) nonidet p-40 solubilized 50-60% and 2% (w/v) sodium deoxycholate solubilized 60-70% of the total membrane protein. as judged by 125i-labelled lentil lectin staining of the sodium dodecyl sulfate--polyacrylamide gel electrophoresis patterns, 2% (w/v) nonidet p-40 ... | 1982 | 7199963 |
| association of membrane and cytoplasmic proteins with the cytoskeleton in blood platelets. | the association of membrane and cytoplasmic proteins with the cytoskeleton of resting and activated platelets was studied. glycoproteins were identified by labeling with 125i-labeled lectins (concanavalin a, wheat germ agglutinin, and lens culinaris). polypeptides, which are embedded in the lipid bilayer, have been identified by their photolabeling with the lipid-soluble reagent 5-[125i]iodonaphthyl 1-azide (125ina). cytoplasmic proteins were identified by their photolabeling with the intracellu ... | 1982 | 7200800 |
| the amino-acid sequence of the alpha subunit of the mitogenic lectin from vicia sativa. | the complete amino acid sequence of the alpha chain of the mitogenic lectin from vicia sativa has been determined. the polypeptide was digested by trypsin and chymotrypsin. the fragments were isolated and most of them were sequenced by automatic solid-phase edman degradation. a total sequence of 52 amino acid residues was obtained which is homologous to the alpha subunits of the lectins from pisum sativum, lens culinaris and vicia faba. the central part (residues 13-32) in particular is complete ... | 1981 | 7202414 |
| induction and specificity of syngeneic antiserum against rauscher murine leukemia virus-induced leukemia, rbl-5. | plasma membranes purified from rbl-5 leukemia cells and solubilized with na deoxycholate were fractionated on an ultrogel aca34 column. fractions containing most of the tumor-rejection activity but low amounts of gp70 and p30 were consolidated and used to hyperimmunize semisyngeneic, cbf1, hybrid mice. using the complement-dependent cytotoxicity assay, we determined the antibody titers against rbl-5 and several other leukemic cells. only rbl-5 and el-4 tumor cells were lysed. similar results wer ... | 1980 | 7203714 |
| differentiation of human alkaline phosphatases by lectin binding affinity. | purified human alkaline phosphatases were separated by lectin binding affinity to wheat germ lectin-sepharose, concanavalin a-sepharose and lentil lectin-sepharose into three isoenzymes: the placental, the intestinal and the liver-bone-kidney-type isoenzyme. therefore, the carbohydrate chains of purified human alkaline phosphatases demonstrate the same isoenzyme classes than studies on structural, catalytical or immunological properties. the liver-bone-kidney-type isoenzyme shows a not yet descr ... | 1980 | 7206590 |
| affinity titration curves: determination of dissociation constants of lectin-sugar complexes and of their ph-dependence by isoelectric focusing electrophoresis. | by performing electrophoresis perpendicular to a stationary ph gradient (ph-mobility curves) in polyacrylamide gels containing a specific ligand either covalently fixed or entrapped in the gel matrix, it is possible to measure dissociation constants (kd) and their ph-dependence in the ph range 3.5-10. the present technique, called 'affinity titration curves', is an extension of 'affinity electrophoresis'. this system has been applied to the study of the interaction between lectins and sugars: le ... | 1980 | 7213653 |
| functional and biochemical characteristics of human "null" lymphoid cells. | human peripheral null cells (non-t and non-b lymphocytes) were isolated by removing b lymphocytes and monocytes on nylon-wool columns and by e rosette-forming cells depletion. the null cell population contained less than 1% of sig-bearing cells, cells with complement receptors (eac+), and e mouse rosette-forming cells, but was contaminated by about 5% of t lymphocytes and 10% of monocytes. high affinity fc receptors were present on 12% of the null cells. no intracytoplasmic immunoglobulins (cigs ... | 1981 | 7222212 |
| the structure of the lentil (lens culinaris) lectin. amino acid sequence determination and prediction of the secondary structure. | the subunit structure and complete amino acid sequence of the lectin extracted from lens culinaris (lcl) seeds was determined. in previous studies, the primary structure of the alpha-chain (mr = 5,710) was shown to be homologous to the alpha-chain of the lectin from pisum sativum, the vicia cracca glucose-specific lectin, and a region in the middle of the concanavalin a sequence (residues 70-121). the complete amino acid sequence of the beta-chain (mr = 17,572) has been determined from 11 trypti ... | 1981 | 7240155 |
| the carbohydrate-binding specificity of pea and lentil lectins. fucose is an important determinant. | the carbohydrate-binding specificities of pea lectin and lentil lectin have been determined by testing the ability of radioactively labeled glycopeptides to bind to columns of pea lectin-sepharose and lentil lectin-sepharose. the presence of a fucose residue attached to the asparagine-linked n-acetylglucosamine residue of the test glycopeptide was essential for high affinity binding to both pea and lentil lectin-sepharose but not to concanavalin a-sepharose. in addition to fucose, 2 alpha-mannos ... | 1981 | 7240233 |
| concanavalin-a binding to acetylcholine receptors: identification of two forms of receptor in denervated rat muscle. | the interaction of the nicotinic acetylcholine receptor from denervated rat muscle (achr, solubilized in triton x-100) and concanavalin-a (con-a) was studied by soluble con-a competing with the binding of achr to agarose immobilized con-a, by immuno-precipitation of con-a-achr complexes and by inhibition of alpha-bungarotoxin binding to achr. results showed that con-a bound to 85% of all achr molecules at multiple cooperative binding sites. con-a caused a partial inhibition of toxin binding to a ... | 1981 | 7241383 |
| specificity of twelve lectins towards oligosaccharides and glycopeptides related to n-glycosylproteins. | glycopeptides and oligosaccharides of either the n-acetyllactosaminic or the oligomannosidic type derived from glycoproteins containing the n-glycosylamine linkage were used to define the specificity of different lectins (concanavalin a, lens culinaris agglutinin, vicia faba agglutinin, pisum sativum agglutinin, ricinus communis agglutinins, soybean agglutinin, wheat germ agglutinin, solanum tuberosum agglutinin, datura stramonium agglutinin, lotus tetragonolobus agglutinin, ulex europeus agglut ... | 1981 | 7262089 |
| the carbohydrate moiety of band 3 glycoprotein of human erythrocyte membranes. structures of lower molecular weight oligosaccharides. | band 3 glycoprotein purified from human blood group a erythrocyte membranes was subjected to hydrazinolysis to release carbohydrate moieties. the neutral oligosaccharide fraction, which constituted more than 85% of the total carbohydrates released, was fractionated by gel chromatography on a column of sephadex g-50 into two fractions, i.e. higher molecular weight and lower molecular weight fractions. from the lower molecular weight fraction, three oligosaccharides were obtained by chromatographi ... | 1981 | 7287720 |
| purification of measles virus glycoproteins and their integration into artificial lipid membranes. | we report a simple method for the isolation of the measles virus glycoproteins, and their subsequent incorporation into artificial lipid bilayers. the two viral glycoproteins, ha and f, were isolated in preparative amounts from disrupted purified virus by lentil lectin affinity chromatography. the proteins were reconstituted into single bilayer lipid vesicles by: (i) exchanging the non-dialysable detergent nonidet p40 (np40) for a dialysable one, octylglucoside, while the proteins were immobiliz ... | 1981 | 7288404 |
| purification of measles virus h polypeptide and of f polypeptide. | measles virus was disrupted by tween 80 and ether and subjected to isoelectric focusing in granular gel. the two surface envelope polypeptides, the one showing haemagglutinating activity (h) and the one making up the structural basis of the haemolytic and fusion activity (f) banded together at ph 5.2. the two envelope polypeptides were also isolated together after adsorption to a lentil-lectin column. separation of the two polypeptides was performed by gel filtration on sephadex g-150 in the pre ... | 1981 | 7295039 |
| purification and characterization of measles virus haemagglutinin protein g. | the 79 000 mol. wt. measles virion membrane glycoprotein g has been isolated from purified measles virus. ultracentrifugation of 2% triton x-100-treated measles virus produced a soluble supernatant fraction containing both g and f, the other external viral membrane protein. lentil lectin-sepharose and sephacryl s-300 column chromatography of this fraction gave a pure preparation of g protein. sucrose density-gradient centrifugation and sds-polyacrylamide gel electrophoresis revealed that g was i ... | 1981 | 7299372 |
| lectin cytochemistry of carbohydrates on cell membranes of rat cerebellum. | wheat germ agglutinin (wga), ricinis communis agglutinin (rca) and lens culinaris (lc) lectins have been used to characterize carbohydrates of neuronal membrane systems in rat and chick cerebellum. wga and rca both label golgi membrane cisternae on the side of the membrane facing the cisternal space but they do not label other elements of the granular and agranular endoplasmic reticulum (er). wga labels the plasma membrane generally and wga binding sites are concentrated within the synaptic clef ... | 1981 | 7310443 |
| characterization of surface antigens of trichinella spiralis infective larvae. | infective larvae of trichinella spiralis were surface-labelled with radioactive iodine, and the products were characterized biochemically and immunochemically. the labelled material was restricted to two basic subunits: a lentil lectin-adherent glycoprotein (gp), mol. wt 47k, and a lentil lectin-nonadherent protein fraction (p), mol. wt 55k. both of these form homologous dimers through as yet unspecified covalent bonds to yield gp90 and p105. gp is further polymerized into higher molecular weigh ... | 1981 | 7322614 |
| characterization of glycophorin a and band 3 from tn polyagglutinable erythrocytes. | the 2 types of erythrocytes from a person with persistent mixed-field polyagglutinability (tn abnormality) were separated from each other by preparative cell electrophoresis. surface labelling using the galactose oxidase/nab3h4 technique followed by polyacrylamide gel electrophoresis showed a strong labelling in the glycophorin a region of tn positive erythrocytes indicating exposed galactosyl n-acetyl/galactosaminyl residues. tn positive cell membranes were labelled by the galactose oxidase/nab ... | 1981 | 7336143 |
| interaction of lentil lectin with human platelets. evidence against glycoprotein ii as aggregation mediator. | 1980 | 7356478 | |
| amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. | we have found a simple procedure to convert epoxy-activated agarose into amino derivatives using ammonia solution. the amino derivatives of agarose were succinylated with succinic anhydride and then activated with n-hydroxysuccinimide according to the method of cuatrecasas and parikh. formula: (see text). coupling of the ligand to the activated agarose (shown above) was performed under mild conditions. the adsorbent thus obtained has no charged group in the linkage region between the ligand and ... | 1980 | 7358652 |
| subcellular compartmentalization of saccharide moieties in cultured normal and malignant cells. | we studied subcellular localization of saccharide moieties in cultured normal and malignant cells fixed in paraformaldehyde and treated with a nonionic detergent, using lectins specific for various surgar residues as probes in fluorescence microscopy. in normal cells, concanavalin a and lens culinaris agglutinin, specific for mannose-rich carbohydrate cores in glycoproteins, labeled the endoplasmic reticulum as a wide perinuclear region. other lectins, on the other hand, stained the golgi appara ... | 1980 | 7372714 |
| lymphocyte recognition of lymph node high endothelium. ii. characterization of an in vitro inhibitory factor isolated by antibody affinity chromatography. | thoracic duct lymph contains a factor that inhibits in vitro adhesion between lymphocytes and high endothelial cells. crude inhibitory factor was isolated from lymph by (nh4)2so4 precipitation and partially purified by using an immunoabsorbant column of rabbit anti peak i ig coupled to sepharose 4b. antibody affinity chromatography separated the hev binding inhibitory factor from the bulk of protein in the crude preparation; activity was enriched 50-fold. the results show that the effect of the ... | 1980 | 7410853 |
| quantitative demonstration of cell surface involvement in a plant-animal symbiosis: lectin inhibition of reassociation. | the freshwater hydra, hydra viridis is normally associated with chlorella-like, algal symbionts which inhabit the host's digestive cells. under experimental conditions bleached hydra will reassociate with algae harvested from green hydra, but not from our cultures of wild type chlorella or strain nc64a which when originally isolated from paramecium bursaria was symbiotically competent. because of its demonstrated selectivity, the reassociation process is hypothesized to involve a recognition int ... | 1980 | 7419619 |
| monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (l-c) antigen of the rat. | the monoclonal antibody (f 10-89-4) described in this study recognizes an antigen which by quantitative absorption analysis is absent from human brain, kidney, liver, heart, erythrocytes, platelets and normal serum, but is present on spleen, lymph node, chronic lymphatic leukemia cells, bone marrow, thymus and granulocytes at a ratio of 1:1:0.8:0.3:0.1, respectively. analysis with the fluorescence-activated cell sorter showed that 100% of thymocytes, lymph node lymphocytes, blood mononuclear cel ... | 1980 | 7428806 |
| human alkaline phosphatases. evidence of three isoenzymes (placental, intestinal and liver-bone-kidney-type) by lectin-binding affinity and immunological specificity. | the structural relationship between human alkaline phosphatase isoenzymes from placenta, intestine, liver, bone and kidney was investigated by lectin-binding affinity chromatography. in addition, antibody-binding sites of the enzymes were studied using monospecific antisera against each of the isoenzymes. evidence is offered for the existence of three classes of alkaline phosphatases: the placental isoenzyme, the intestinal isoenzyme and the liver-bone-kidney-type-isoenzyme: 1. a high affinity t ... | 1980 | 7437449 |
| [applicability of concanavalin a and lectin from the lens culinaris. histochemical study (author's transl)]. | 1980 | 7437917 | |
| heymann nephritis induced by kidney brush border glycoproteins. | heymann nephritis developed in rats immunized with brush border membrane fractions isolated from rat kidney tubules. glomerular autoantibodies eluted from cryostat sections of nephritic kidneys reacted in immunoelectron microscopy with the outer surface of isolated brush border membrane vesicles. this indicates that the autoantigens are plasma membrane components. to characterize further the chemical nature of the nephritogenic autoantigens, we treated the brush border membranes with trypsin and ... | 1980 | 7442131 |
| detection of lectin-sugar interaction by ultraviolet difference spectroscopy. | it was found that the addition of specific sugars to solutions of several lectins induced ultraviolet difference spectra. the difference spectra of lectins from lens culinaris, sophora japonica, solanum tuberosum and wheat germ have two peaks at 292 nm and 284-287 nm which are characteristic of the tryptophanyl residue. the difference spectrum of arachis hypogaea agglutinin has two peaks at 285 nm and 279 nm which seem to be characteristic of the tyrosyl residue. in addition to the identificatio ... | 1980 | 7451408 |
| molecular profiles of human melanoma-associated antigens. | melanoma-associated antigens (maa) shed into spent culture medium of intrinsically radiolabeled melanoma cells react specifically with monoclonal and polyclonal anti-melanoma xenoantiserums and are represented by two glycoproteins with molecular weights of 240,000 (240k) and 94,000 (94k): 240k is present only on melanoma cells whereas 94k is also found on carcinoma cells and on fetal melanocytes. both 240k and 94k have been obtained radiochemically pure by utilizing cellulose ion-exchange and an ... | 1981 | 7461145 |
| galactose oxidase labeling of membrane proteins from human brain white matter. | the use of galactose oxidase (ec 1.1.3.9) and tritiated sodium borohydride for labeling of membrane glycoproteins, described by gahmberg and hakomori, has previously been applied to the study of myelin glycoproteins of experimental animals. rat brain myelin glycoproteins have been studied by sequential lectin affinity of chromatography and recently the lectin-binding capacity of rat central nervous system myelin glycoproteins has been characterized. complex heterogeneity of the glycoprotein patt ... | 1981 | 7470863 |
| prolonged effects of tumor necrosis factor-alpha on anterior pituitary hormone release. | we examined the chronic (72 h) effects of 30 ng/ml recombinant murine tumor necrosis factor (tnf)-alpha on release of immunoreactive growth hormone (gh), prolactin (prl), thyrotropin (tsh), and tsh glycosylation, as assessed by lectin binding, in cultured rat anterior pituitary cells. in cultured cells from adult female rats, tnf-alpha significantly suppressed basal and gh-releasing hormone (grh)-stimulated gh release. tnf-alpha also suppressed basal prl release and completely abolished the prl ... | 1995 | 7479297 |
| carbohydrate-based measurements on alpha-fetoprotein in the early diagnosis of hepatocellular carcinoma. | serum alpha-fetoprotein (afp) is a useful marker for the diagnosis of hepatocellular carcinoma (hcc), although this protein also increases moderately in benign liver diseases. the serum concentration of afp in hcc at the time of initial diagnosis is now lower than before because of advancements in techniques for imaging the liver. the afp concentration alone cannot distinguish between hcc and benign liver diseases, especially when it is less than 1000 ng ml-1. these circumstances lead to the nee ... | 1995 | 7496131 |
| alpha-fetoprotein glycosylation is abnormal in some hepatocellular carcinoma, including white patients with a normal alpha-fetoprotein concentration. | lectin-affinity analyses with lens culinaris agglutinin (lca) and other lectins have demonstrated that the glycosylation of alpha-fetoprotein (afp) secreted by hepatocellular carcinomas (hcc) is frequently altered when the serum afp concentration is increased. to determine if afp lca-binding properties are altered in patients with hcc whose serum afp concentration is normal, the percentage of lca-binding afp in serum from white newborns, white normal adults, white patients with chronic hepatitis ... | 1993 | 7506988 |
| [a case of nonseminomatous germ cell tumor: usefulness of alphafetoprotein subfraction]. | alfa-fetoprotein (afp) is an indispensable examination in the management of non-seminomatous germ cell tumor. however, many liver diseases also frequently show the elevation of afp. therefore, it is essential to discriminate between yolk sac-derived component and liver-derived one. a 32-year-old male who had suffered from chronic hepatitis, visited our clinic in december, 1991. he complained of left scrotal atrophy and dull pain. surgical specimen was histologically diagnosed as embryonal carcin ... | 1994 | 7514845 |
| tissue treatment for whole mount internal lectin staining in the nematodes caenorhabditis elegans, panagrolaimus superbus and acrobeloides maximus. | four different fixation schemes, using ten fluorescent-labelled lectins, were investigated for whole mount internal staining of three rhabditid nematodes: caenorhabditis elegans, panagrolaimus superbus and acrobeloides maximus. acetone-only fixation was found to give strong and reproducible staining, which could be prevented either by periodate treatment of the organisms or by specific inhibitory sugars of the lectins under investigation. whereas the use of either phosphate or tris buffers had n ... | 1994 | 7523338 |
| role of cd59 in experimental glomerulonephritis in rats. | cd59 is a molecule which is present on the host cell membranes and inhibits formation of membrane attack complex. a monoclonal antibody, 6d1, recognizes a rat analogue of human cd59. 6d1 inhibits function of rat cd59 and can enhance complement-mediated hemolysis in vitro. to assess the role of cd59 in complement-mediated glomerular injury, 6d1 was tested in a model of experimental glomerulonephritis induced by a lectin and its antibodies. the left kidney of a rat was perfused either with 200 mic ... | 1994 | 7523753 |
| clinical significance of serum alpha-fetoprotein subfractionation in pediatric diseases. | serum alpha-fetoprotein (afp) subfraction profile is a predictive indicator for the discrimination of hepatic malignancies, benign liver diseases and yolk sac tumor in adults. in the present study, afp subfractions were examined in afp-positive sera from 59 patients of less than 15 years of age. fractionation of afp was carried out by lectin affinity crossed-line immunoelectrophoresis. concanavalin a, lens culinaris hemagglutinin and phytohemagglutinin e were used as lectins. fifty-four of 59 (9 ... | 1994 | 7524830 |
| porcine mitogen-induced interferon. | induction of porcine interferon (poifn) with different lectins (phytohaemagglutinin, lens culinaris lectin, arachis hypogea lectin) in various concentrations (500, 50 and 5 micrograms/ml) was studied. kinetics of poifn appearance was analyzed by antiviral and antiproliferative tests in human transformed and nontransformed cells. the relation between antiviral and antiproliferative activity of poifn in terms of respective units was determined. poifn was characterized in comparison with human inte ... | 1994 | 7526670 |
| distribution of carbohydrates in the zona pellucida of human oocytes. | the purpose of the present study was to investigate the distribution pattern of carbohydrates in the zona pellucida of human oocytes using lectins and ruthenium red as histochemical probes. for lectin analyses, oocytes that failed to undergo fertilization following in vitro insemination were collected, washed, fixed with glutaraldehyde and embedded in araldite. for ruthenium red labelling, the oocytes were fixed with glutaraldehyde containing ruthenium red, post-fixed with oso4 and embedded in a ... | 1994 | 7528279 |
| analysis of alpha-fetoprotein by a procedure combining crossed immunoaffinoelectrophoresis and immunoblotting. | a newly developed procedure combining crossed immunoaffinoelectrophoresis and an immunoblotting assay (ciae-iba) has been used to discriminate between and precisely quantitate lectin-bound and lectin-free forms of alpha-fetoprotein (afp) derived from human serum at levels of afp as low as 5.6 ng/ml (adult reference range, 0 to 15). twenty-two serum specimens with elevated levels of afp were examined by a reference ciae method, using either of two lectins, concanavalin a (con a) or lens culinaris ... | 1995 | 7540383 |
| lectin and immunohistochemical comparison of glycoconjugates in the conjunctiva of patients with and without exfoliation syndrome. | aims--the study was carried out to search for labelling similar to that of intraocular exfoliation material in the conjunctiva by light microscopy using lectin and immunohistochemistry. methods--ten formalin fixed and paraffin embedded conjunctival biopsy specimens both from patients with and without exfoliation syndrome were studied with a panel of 11 lectins and with three monoclonal antibodies to the hnk-1 carbohydrate epitope, all of which react with intraocular exfoliation material. results ... | 1995 | 7542024 |
| biochemical characteristics and clinical applications of alpha-fetoprotein isoforms. | alphafetoprotein (afp) is an oncofetal glycoprotein that frequently reappears in sera of patients affected by hepatocellular carcinoma (hcc) and yolk-sac tumours (yst). in these cases afp determination represents a useful marker for monitoring the therapeutic response and the clinical evolution of the neoplastic disease. afp is a heterogeneous molecule with respect to the carbohydrate moiety and the different afp glycoforms can be separated and characterized by their affinity for lectins, such a ... | 2006 | 7544570 |
| a clinical study of lectin-reactive alpha-fetoprotein as an early indicator of hepatocellular carcinoma in the follow-up of cirrhotic patients. | levels of two types of lectin-reactive alpha-fetoprotein (afp), designated afp-l3 and afp-p4+p5, were analyzed with lens culinaris agglutinin a and afp-p4+p5 with erythroagglutinating phytohemagglutinin, respectively, in an attempt to determine the utility and significance of these macromolecules as early indicators of hepatocellular carcinoma during the periodic follow-up of cirrhotic patients. the subjects were 51 of 190 consecutive cirrhotic patients in whom hepatocellular carcinoma developed ... | 1995 | 7544756 |
| murine tumorlytic factor, immunologically distinct from tumor necrosis factor-alpha and -beta, induced in the serum of mice treated with a t-cell mitogen of corynebacterium kutscheri. | murine tumorlytic factor (tf), immunologically distinct from murine tumor necrosis factor (tnf)-alpha and -beta, was purified to a homogeneity from the serum of mice injected with a t-cell mitogen of corynebacterium kutscheri. the treated mouse serum was purified by lentil lectin-sepharose chromatography, deae-cellulose chromatography, preparative isoelectric focusing, and high-pressure liquid chromatography to the specific activity of 1.5 x 10(6) u/mg protein. tf was 42 kda in its oligomeric fo ... | 1995 | 7590903 |
| glycoconjugate expression of chondrocytes and perichondrium during hyaline cartilage development in the rat. | alterations in the expression of glycoconjugate structures during cartilage development in the chondrocranium, nasal skeleton, meckel's cartilage, limb buds, vertebral bodies and ribs were investigated comparatively in 13 to 21-d-old rat embryos. the binding patterns of 24 biotinylated lectins were analysed in serial sections and compared with results obtained using histochemical methods. proteoglycan distribution, assessed by conventional staining procedures, was not associated with lectin bind ... | 1995 | 7591987 |
| lectins inhibit the aujeszky's disease virus-induced interferon-alpha production of porcine peripheral blood mononuclear cells. | the interaction between virus and peripheral blood mononuclear cells (pbmc) required to elicit the production of interferon-alpha (ifn-alpha) by the so-called natural interferon-producing cell is unknown. however, results from inhibition experiments suggest that viral glycoproteins are essential in this ifn induction process. we demonstrate here that cellular glycoproteins also appear to be involved in the initiation of ifn-alpha production. lectins, that is, sugar binding glycoproteins, inhibit ... | 1995 | 7627804 |
| effect of retinoic acid on the structure of n-glycans on the surface of human hepatocarcinoma cells and its enzymatic mechanism. | in order to study the effect of retinoic acid on the structure of n-glycans on the cell surface, the n-glycans of glycoproteins on the surface of 7721 human hepatocarcinoma cells were labelled with [3h] mannose, added to the culture medium. the 3h-labelled n-glycans were prepared from cell-surface glycoproteins, desialylated, and subjected to sequential chromatography on concanavalin a and datura stranonium agglutinin affinity columns to separate the glycans into four fractions of different type ... | 1995 | 7635868 |
| carbohydrate structures of human alpha-fetoprotein of patients with hepatocellular carcinoma: presence of fucosylated and non-fucosylated triantennary glycans. | chemical structures of the sugar chains of various human alpha-fetoprotein (afp) species with different affinity for concanavalin a (con a) and lens culinaris agglutinin (lca) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion. using reversed-phase and size-fractionation high performance liquid chromatography systems we identified six pyridylamino-sugar chains. the con a-reactive and lca-nonreactive species of afp from patients with hepatocellular c ... | 1993 | 7679920 |
| establishment of assay kits for the determination of microheterogeneities of alpha-fetoprotein using lectin-affinity electrophoresis. | diagnostic kits for determination of alpha-fetoprotein (afp) carbohydrate chain microheterogeneity were developed using lectin affinity electrophoresis with lens culinaris agglutinin-a (lca-a) and erythro-agglutinating phytohemagglutinin-e4 (pha-e4). separated afp bands by electrophoresis were detected with high sensitivity by antibody-affinity blotting and immunoenzymatic amplification. densitometry was used to apportion lectin reactive afps. the within-run s.d. for proportions of afp bands was ... | 1993 | 7680970 |
| early recognition of hepatocellular carcinoma based on altered profiles of alpha-fetoprotein. | the sugar-chain structures of circulating alpha-fetoprotein in patients with hepatocellular carcinomas differ from those in patients with cirrhosis. we studied the reactivity of alpha-fetoprotein with two lectins, lens culinaris agglutinin a and erythroagglutinating phytohemagglutinin, to monitor the evolution of hepatocellular carcinoma in patients with cirrhosis. | 1993 | 7684823 |
| highly enhanced fucosylation of alpha-fetoprotein in patients with germ cell tumor. | we have recently reported that simultaneous determination of the fucosylation and glucosaminylation of alpha-fetoprotein (afp) was useful in the differential diagnosis of various liver diseases. here, we measured these indices in germ cell tumor and compared them with those in various diseases to reveal the characteristic feature of the carbohydrate moiety of afp in this tumor. | 1993 | 7686444 |
| serum alpha-fetoprotein and lens culinaris agglutinin-reactive fraction of alpha-fetoprotein in patients with hepatocellular carcinoma. | a fraction of serum alpha-fetoprotein (afp) reactive with lens culinaris agglutinin (lca) was measured by affinity chromatography in serum samples from 102 patients with hepatocellular carcinoma (hcc) and 48 patients with chronic liver diseases without hcc. its usefulness as a marker of hcc was evaluated. the mean +/- sd percentage of this fraction in total afp was 3.10 +/- 3.17% in 48 patients with chronic liver diseases without hcc. when the cut-off level was set at 12.6% (mean + 3 sd), the se ... | 1993 | 7690873 |
| a collaborative study for the evaluation of lectin-reactive alpha-fetoproteins in early detection of hepatocellular carcinoma. | lectin-affinity electrophoretic separation of serum alpha-fetoprotein (afp) was carried out using afp differentiation kits, which used lens culinaris agglutinin-a (kit l) and erythroagglutinating phytohemagglutinin (kit p). separated afp bands were detected with a sensitive antibody-affinity blotting technique and determined quantitatively by densitometry, and the results were expressed as percentages of the intensity of total afp bands. sera from 424 patients with acute hepatitis, chronic hepat ... | 1993 | 7693340 |
| characterization of glycoconjugate expression during development of meckel's cartilage in the rat. | the staining patterns of 24 biotinylated lectins were analyzed in serial sections of the mandible of 13- to 21-day-old rat embryos by means of the avidin-biotin-peroxidase method. a ubiquitous distribution of binding sites was demonstrated after incubation with con a (canavalia ensiformis), dsl (datura stramonium; except bone matrix), and wga (triticum vulgare). ecl (erythrina cristagalli), gsl i (griffonia simplicifolia), sja (saphora japonica), vvl (vicia villosa), dba (dolichus biflorus), uea ... | 1995 | 7717533 |
| the diagnostic importance of species specific and cross-reactive components of taenia solium, echinococcus granulosus, and hymenolepis nana. | sera from patients infected with taenia solium, hymenolepis nana and echinococcus granulosus were tested against homologous and heterologous parasite antigens using an elisa assay, and a high degree of cross-reactivity was verified. to identify polypeptides responsible for this cross reactivity, the enzyme linked immunoelectro transfer blot (eitb) was used. sera from infected patients with t.solium, h.nana, and e.granulosus were assessed against crude, ammonium sulphate precipitated (tsasp), and ... | 1994 | 7732263 |
| role of lipid peroxidation in electroporation-induced cell permeability. | erythroleukemia k562 cells and lentil (lens culinaris) root protoplasts have been subjected to pore-forming electric fields suitable for transfection experiments. evidence is presented that the amount of hydroperoxides formed in cell membranes of both cell-types is a function of field strength applied. on the other hand, the electroporation-induced lipid peroxidation paralleled the enhancement of membrane permeability and was associated with greater membrane fluidity. the membrane hydroperoxides ... | 1995 | 7733908 |
| aberrant pattern of lectin binding in low and high grade prostatic intraepithelial neoplasia. | high grade prostatic intraepithelial neoplasia (pin) is considered a precursor lesion for peripheral prostatic adenocarcinoma. the biologic significance of low grade pin is unknown; however, the likelihood of progression from low to high grade pin has been suggested. | 1995 | 7736399 |
| serum thyrotropin (tsh) heterogeneity in euthyroid subjects and patients with subclinical hypothyroidism: the core fucose content of tsh-releasing hormone-released tsh is altered, but not the net charge of tsh. | the aims of the present study were to determine the influence of brief subclinical hypothyroidism on the isoforms of serum thyrotropin (tsh) and to examine the net charge of tsh in different metabolic states. sera were obtained from euthyroid subjects (n = 7) and from patients with subclinical hypothyroidism (n = 8) before and 30 min after the intravenous administration of 200 micrograms thyrotropin-releasing hormone (trh). the tsh from human pituitary extracts (irp 68/38), basal and trh-stimula ... | 1995 | 7738480 |
| [lectin-binding proteins of plasma membranes of a human trophoblast]. | spectra of lectin-binding proteins of the human trophoblast were studied by the method affinity blotting using a set of loctins conjugated with peroxidase. the set included lectins lens culinaris (lcl), canavalia ensiformis (con a), ricinus communis (rca 1), arachis hypogaea (pna), triticum vulgaris (wga), laburnum anagyroides (lal). presence of n- and o-glycans in the composition of proteins of plasmatic membranes of a trophoblast was shown, partial characteristics of their oligosugar chains be ... | 1994 | 7747337 |
| duck lymphocytes. vii. selection of subpopulations using lectin-coated magnetic beads. | the optimum conditions (time, concentration of duck serum, ratio of cells:beads) for binding of duck lymphocytes to magnetic beads (dynabeads) coated with the lectins phytohaemagglutinin (pha), wheat germ agglutinin (wga), lentil lectin (ll) and concanavalin a (con a) were determined. in blood and lymphoid organs, the largest population was cells binding pha (55-80%) and the smallest was cells binding con a (0-15%) with cells binding wga and ll occurring at 15-58%. most blood lymphocytes had rec ... | 1995 | 7747400 |
| drosophila insulin receptor: lectin-binding properties and a role for oxidation-reduction of receptor thiols in activation. | the glycosylation of the drosophila insulin receptor (dir) has been compared to that of the rat insulin receptor by examining the binding of receptors to the lectins wheat germ agglutinin, concanavalin-a, and lentil lectin. although rat insulin receptors bound and were specifically eluted from all three lectins, only a small fraction of the dir (< 5%) was retained on wheat germ agglutinin. in contrast, the dir bound strongly to concanavalin-a and lentil lectin and was recovered from lentil lecti ... | 1995 | 7750456 |
| a rapid lectin receptor binding assay: comparative evaluation of sea urchin embryo cell surface lectin receptors. | lectin receptor binding assays, such as those that utilize fluorescence, radioactivity or electron microscopy are not designed for rapidly screening hundreds of cell types for the presence or absence of specific lectin receptors. an assay is described here that is designated for this purpose. it utilizes lectins derivatized to agarose beads and can be used to screen many cell types in min. this assay was used to examine lectin receptors on the surfaces of 1-8 cell stage strongylocentrotus purpur ... | 1995 | 7771187 |
| characterization of the atpase activity of p-glycoprotein from multidrug-resistant chinese hamster ovary cells. | p-glycoprotein (pgp) was isolated from chrc5 membranes by selective detergent extraction and further purified by lentil lectin affinity chromatography. the purified product displayed a very high basal atpase activity (1.65 mumol/min per mg protein in the absence of added drugs or lipids) with an apparent km for atp of 0.4 mm. there was no evidence of cooperativity, suggesting that the two atp sites operate independently of each other. pgp atpase activity was stimulated by verapamil, trifluoperaz ... | 1995 | 7772017 |
| biogenic amines and microbial quality of sprouts. | changes in the biogenic amine content relative to microbial activities in mung bean, lentil and radish sprouts were investigated in prepacked and "home-grown" products. biogenic amines were determined by ion-exchange chromatography. the major groups of micro-organisms were enumerated by aerobic plate count procedures, using universal and selective media. putrescine, cadaverine, spermidine, agmatine and spermine were detected in different concentrations, depending on the type of sprouts. in prepa ... | 1995 | 7785356 |
| [effect of concanavalin a on morphology and dna synthesis of resting chondrocyte cultures]. | rabbit chondrocytes were isolated from resting cartilage of ribs of 4-week-old new zealand rabbits. chondrocytes were seeded at low density and grown to confluency in medium (dme) with 10% fetal bovine serum, 50 micrograms/ml ascorbic acid, and antibiotics, at 37 degrees c under 5% co2 in air, and then serum concentration was reduced to 0.3%. at the low serum concentration, chondrocytes adopted fibroblastic morphology. addition of concanavalin a to the culture medium induced a morphologic altera ... | 1994 | 7801723 |
| lentil root protoplasts: a transient expression system suitable for coelectroporation of monoclonal antibodies and plasmid molecules. | protoplasts were isolated from lentil (lens culinaris) roots and their suitability as a transient expression system was investigated. after transfecting the protoplasts with the beta-glucuronidase (gus) gene by either electroporation or polyethylene glycol (peg), the specific activity of the reporter enzyme and the cell viability were determined. electroporation was more effective than peg treatment as transfection procedure and its efficiency was affected by the plasmid length. the feasibility ... | 1995 | 7827102 |
| effect of lectins on mouse peritoneal macrophage phagocytic activity. | we studied the in vitro ability of lectin-treated murine peritoneal macrophages to attach and phagocytize particulate antigens. glucose and mannose specific lectins such as con-a and lentil lectin, as well as complex lactosamine residues specific lectins, such as phaseolus vulgaris var. cacahuate and phaseolus coccineus var. alubia, increased the macrophage phagocytic activity towards heterologous erythrocytes, whereas peanut agglutinin, a galactose-specific lectin, diminished the macrophage pha ... | 1994 | 7851961 |
| biochemical characterization of sheep platelet acetylcholinesterase after detergent solubilization. | the biochemical characterization of detergent-solubilized acetylcholinesterase (ache) from subcellular particles of sheep platelets and the effects of different effectors on ache activity from solubilized platelet crude membranes have been undertaken and studied. solubilization of ache with detergent increased the thermal stability of the enzyme from all particulate fractions. solubilized ache from the mitochondria-granule fraction was the most thermostable at 55 degrees c. the km values against ... | 1995 | 7858952 |
| [nutritional use of lentil proteins in the young rat]. | the nutritional utilization of the lentil was compared to that of casein, used as the only protein food in young rats at two different ages: weanling and 10 days thereafter. cooking allowed a better intake and digestive utilization of lentil crude protein by the weanling rats. in this case, methionine showed to be the first limiting factor. when lentil diets were given to the older rats, their intake and growth rates reached the level of the control rats. in this case, the free amino acid pools ... | 1995 | 7872652 |
| [stimulation of maturing and terminal differentiation by concanavalin a in rabbit permanent chondrocyte cultures]. | the effect of concanavalin a (con a) on maturing and terminal differentiation in permanent chondrocyte cultures were examined. chondrocytes isolated from permanent cartilage were seeded at low density and grown in mem medium containing 10% fetal bovine serum, 50 micrograms/ml of ascorbic acid and antibiotics, at 37 degrees c under 50% co2 in air. at 0.3% of low serum concentration, addition of con a to the culture medium increased by 3- to 4-fold the incorporation of [35s] sulfate into large cho ... | 1994 | 7879571 |
| loss of biological activity of arginine vasopressin during its degradation by vasopressinase from pregnancy serum. | degradation of avp by placental vasopressinase may precipitate gestational diabetes insipidus, which in some cases is accompanied by pre-eclampsia. abnormally elevated vasopressinase has also been reported in pre-eclampsia without diabetes insipidus. this association between excessive vasopressinase production and pre-eclampsia might be explained if the products of avp degradation by vasopressinase retained pressor activity even after anti-diuretic activity had been destroyed. recent evidence in ... | 1995 | 7889632 |
| characterization of human arylsulfatase a glycans. | despite numerous studies on arylsulfatase a, the structure of the glycans present in each of its two subunits has not been determined. this is important because the carbohydrate component of human arylsulfatase a synthesized in tumor tissues and transformed cells has been shown to undergo apparent changes. this study elucidates some of their major features. glycan chain analysis of native and deglycosylated arylsulfatase a as well as its subunits was performed with the use of a glycan differenti ... | 1994 | 7890120 |
| exogenously added copper does not increase the production of copper amine oxidase in lentil seedlings. | the effect of copper ions, the inorganic cofactor of amine oxidase (ao; ec 1.4.3.6), on the production of this enzyme in lentil (lens culinaris) seedlings was studied. the addition of cuso4 to the imbibition water during the germination of lentils increased the ao activity. however, the amounts of specific mrna and protein were not affected by the presence of cu2+. furthermore, the addition of cu2+ to homogenates of lentil seedlings grown in the absence of the metal substantially increased the a ... | 1995 | 7890159 |
| determination of broad bean stain virus serotypes by enzyme-linked immunosorbent assay. | enzyme-linked immunosorbent assay (elisa) was used to determine the degree of serological specificity of two serotypes of broad bean stain virus (bbsv) and their relationship to red clover mottle virus (rcmv). optimal conditions for the differentiation by elisa of the two viruses and the two bbsv serotypes were established. bbsv isolates from vicia sativa belonged to serotype i, those from pea plants (f1, kow 60) to serotype ii, and isolates from lens culinaris differed from these two serotypes. ... | 1993 | 7905241 |
| reactivity in elisa and dot blot of purified gp24, an immunodominant antigen of taenia solium, for the diagnosis of human neurocysticercosis. | in this paper we report the purification of gp24, one of the seven specific and highly antigenic taenia solium glycoproteins previously identified by western blot (wb) with serum, cerebrospinal fluid (csf) and saliva samples from patients with neurocysticercosis (nc). gp24 was purified and evaluated in elisa and dot blot for diagnosis. a lentil-lectin-bound glycoprotein fraction (ll-gp) from t. solium cysticerci was submitted to polyacrylamide gel electrophoresis, and the band that corresponded ... | 1994 | 7928076 |
| magnetic resonance imaging of antibody-mediated demyelinating experimental allergic encephalomyelitis. | two models of demyelinating experimental allergic encephalomyelitis (eae) were studied on lewis rats in whom the disease was induced by injections of either (i) lentil-lectin binding myelin glycoproteins plus myelin basic protein (mbp)-specific t cells (36 rats), or (ii) myelin/oligodendrocyte glycoprotein-specific monoclonal antibody plus mbp-specific t cells (16 rats). in our 24 control rats, 20 received mbp-specific t cells only, and four received myelin glycoproteins plus purified protein de ... | 1994 | 7929802 |
| a method of purifying feline t lymphocytes from peripheral blood using the plant lectin from pisum sativum. | the binding properties of several plant lectins on feline peripheral blood lymphocytes (pbl) were examined by flow cytometry to reveal if any could serve as probes for identifying specific lymphocyte subclasses. most of the lectins bound > or = 90% of pbl in a uniform manner. the exceptions were the lectins from glycine max, phaseolus limensis, and dolichos biflorus, which bound only a proportion of the pbl. the differential binding of the lectins to lymphocyte subclasses was examined by compari ... | 1994 | 7930648 |
| molecular cloning and in situ localization of the human contactin gene (cntn1) on chromosome 12q11-q12. | chick contactin/f11 (also known as f3 in mouse) is a neuronal cell adhesion molecule of the immunoglobulin (ig) gene family that is implicated in playing a role in the formation of axon connections in the developing nervous system. in human brain, contactin was first identified by amino terminal and peptide sequencing of the lentil-lectin-binding glycoprotein gp135. we now report the isolation and characterization of cdna clones encoding human contactin. human contactin is composed of six c2 ig- ... | 1994 | 7959734 |
| purification of the synaptic vesicle-binding protein physophilin. identification as 39-kda subunit of the vacuolar h(+)-atpase. | physophilin is a 36-kda polypeptide originally identified in synaptic plasma membrane fractions, which binds to synaptic vesicles and has been implicated in vesicle docking and/or exocytosis during neurotransmitter release. here we report on the purification, amino acid sequence analysis, and subcellular localization of physophilin. physophilin was enriched from detergent extracts of crude synaptic plasma membranes by a combination of cation exchange and lentil-lectin chromatography. sequence an ... | 1994 | 7961771 |
| a protective "concealed" antigen from boophilus microplus. purification, localization, and possible function. | a membrane protein that can be used successfully to vaccinate cattle against the tick boophilus microplus has been purified and characterized. the mature protein, which is referred to as bm91, has an apparent m.w. of 86,000, an isoelectric point of 4.8 to 5.2, and is glycosylated, with an affinity for lentil lectin. bm91 is of relatively low abundance, with approximately 300 to 400 micrograms being recovered from 1 kg of semiengorged adult female ticks. the protein is located largely in the sali ... | 1994 | 7963573 |
| isolation and characterization of a diagnostic polysaccharide antigen from larval echinococcus multilocularis. | a diagnostic antigen, c-antigen, was evaluated in serodiagnosis of alveolar hydatid disease by western blotting. the c-antigen migrated diffusely to the region of 30-35 kda in 8% polyacrylamide-sds gel electrophoresis. we isolated this antigen from a crude metacestode extract by a simple method of phenol/chloroform extraction. the isolated antigen was resistant to boiling, proteolysis and acid hydrolysis, but destroyed with sodium periodate. such lectins as peanut agglutinin (pna), ricinus commu ... | 1994 | 7967161 |
| fibre constituents of some foods. | some plant foods viz. bottlegourd, carrot, cauliflower, cabbage, green bengalgram, pea, apple, plum, guava, karonda, blackgram husk and lentil husk were analysed for their dietary fibre components. the total dietary fibre contents of these foods varied from 14.68 to 78.21 percent on dry matter basis. as compared to fruits and vegetables, the husks had higher amount of total dietary fibre. cellulose represented as the major fibre constituent in most of the foods whereas, husks were observed to be ... | 1994 | 7971775 |
| insoluble dietary fiber of grain food legumes and protein digestibility. | the present work aimed to verify the digestibility of cooked whole food grain legumes. samples of beans (phaseolus vulgaris and vigna sinensis), chickpeas (cicer arietinum) and lentils (lens culinaris) were used in the experiment. the interrelationship between the insoluble dietary fiber presented in the food grain legumes and the low protein digestibility was studied. the insoluble dietary fiber and the proteic nitrogen presented in the neutral detergent fiber (ndf) were determined. "in vivo" d ... | 1993 | 8002707 |
| chemical and immunological analysis of the aspergillus fumigatus cell wall. | hyphal-wall preparations of aspergillus fumigatus have been analysed by sequential treatment with koh, nitrous acid and again with koh. by acidification of the alkali-soluble extract, a polyglucose was precipitated which showed an x-ray diffraction pattern similar to that of (1-->3)-alpha-glucan. the remainder of the alkali-soluble fraction was precipitated with ethanol; it contained all the mannose, galactose and protein of the wall and, in addition, 6.2% of the amino sugars. this wall-associat ... | 1994 | 8012598 |
| isolation and analysis of lectin-reactive sarcoma-associated membrane glycoproteins. | sarcoma and normal tissue plasma membrane lectin-reactive glycoproteins were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. two peanut agglutinin-reactive n-acetylgalactosamine-containing glycoproteins of 1.05 x 10(6) and 1.25 x 10(5) da and one lentil agglutinin-reactive mannose/n-acetylglucosamine(-fucose)/sialic acid-containing glycoprotein of 1.7 x 10(5) da (gp170) were detected in osteosarcoma and malignant fibrous histiocytoma (mfh), respectively. however, these gly ... | 1994 | 8017864 |
| characterization of a novel family of ciliary body glycoproteins. | to isolate and characterize ciliary body epithelial antigens reactive with a monoclonal antibody, 2b4.14.1. | 1994 | 8045720 |
| purification of factor viii/von willebrand factor from human plasma on immobilized lentil lectin. | human factor viii/von willebrand factor (fviii/vwf) was shown to bind to immobilized lectins from arachis, ulex, concanavalia, and lens species. the protein/lectin interaction displayed higher affinities for the lectins from the last two species. however, the lens culinaris lectin immobilized on sepharose 4b (lca-sepharose) provided a more selective and flexible affinity system for the purification of fviii/vwf than concanavalia lectin. chromatography on lca-sepharose of a purified fviii contain ... | 1994 | 8054845 |
| isolation of a 32 kda mycobacterium tuberculosis protein by lectin affinity chromatography. | a 32 kda antigen from delipidated m. tuberculosis h37rv culture filtrate protein extract (cfpe) was purified by affinity chromatography on immobilized lens culinaris lectin and electroelution. this antigen represents 0.4% of the total cfpe carbohydrate content and possesses galactose, xylose, mannose and glcnac (5:2:3:1 mol. ratio). a monoclonal antibody against the purified antigen reacted with the 32 kda as well as a 30 kda antigen in h37rv cfpe, thus suggesting that both antigens represent cl ... | 1994 | 8055192 |
| lectin histochemistry of infantile lysosomal storage disease associated with osteopetrosis. | in infantile lysosomal storage disease associated with osteopetrosis the nature of the enzyme deficiency as well as the type of material accumulated are both unknown. we used lectin histochemistry to characterize the storage material of previously reported cases. using paraffin sections neurons stained positively with luxol fast blue (lfb), periodic acid-schiff (pas), concanavalia ensiformis agglutinin, datura stramonium agglutinin, griffonia simplicifolia-i, lens culinaris agglutinin, ricinus c ... | 1994 | 8091952 |