Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [severe genital mycoplasma infection following cesarean section]. | authors report a serious case of post-caesarean delivery endometritis caused, probably exclusively, by genital mycoplasmas: ureaplasma urealyticum and mycoplasma hominis. the initial treatment of the patient with various penicillins, ceftriaxone, gentamicin, metronidazole and nystatine proved ineffective. subsequently, as microbiological tests turned out positive for genital mycoplasmas, a therapy of doxycyclin was introduced and a full recovery could be attained. authors' experience is consiste ... | 1998 | 9545798 |
| microbiology of acute arthropathies among children in argentina: mycoplasma pneumoniae and hominis and ureaplasma urealyticum. | to describe the isolation of mycoplasmas and ureaplasmas from synovial fluid in pediatric patients with joint disorders. | 1998 | 9576384 |
| [appearance of inflammatory cytokines interleukin-1 beta and interleukin-6 in amniotic fluid during labor and in intrauterine pathogen colonization]. | subclinical intrauterine infections have been proposed to be one of the leading causes of preterm labor. the determination of inflammatory cytokines il-1 beta and il-6 in the amniotic fluid may be useful to identify women who have infection-associated preterm labor. amniotic fluid was collected from 99 women during amniocentesis, during cesarean section or at the time of amniotomy using sterile technique. il-1 beta and il-6 were determined by a specific elisa. fluid of each sample was cultured f ... | 1998 | 9577917 |
| [detecting ureaplasma urealyticum, mycoplasma hominis and mycoplasma genitalium in the urogenital tract]. | to epidemiological study of ureaplasma urealyticum (uu), mycoplasma hominis (mh) and mycoplasma genitalium (mg) in the urogenital tract of different groups of people. | 1997 | 9596936 |
| successful treatment of infection due to mycoplasma hominis with streptogramins in a renal transplant patient: case report and review. | 1998 | 9597262 | |
| elevated interleukin-8 concentrations in amniotic fluid of mothers whose neonates subsequently develop bronchopulmonary dysplasia. | to determine if an intrauterine sub-clinical inflammatory process is a risk factor for the development of bronchopulmonary dysplasia. | 1998 | 9605441 |
| influence of urogenital infections on sperm functions. | many studies have examined the impact of genital tract infections on male fertility; however, the effect of bacteriospermia on sperm quality is still controversial. bacterial infections are more frequently found in semen samples from asymptomatic infertile patients than in those from fertile men. bacteriospermia is also a common problem of male partners from couples undergoing ivf. therefore, the effects of microorganisms on human sperm acrosome reaction of oocytes have been studied in vitro and ... | 1998 | 9629446 |
| inhibition of human peripheral blood mononuclear cell proliferation by streptococcus pyogenes cell extract is associated with arginine deiminase activity. | streptococcus pyogenes (group a streptococcus) cell extracts (ce) have a remarkably powerful and dose-dependent inhibitory effect on antigen, superantigen, or mitogen-stimulated human peripheral blood mononuclear cell (pbmc) proliferation in vitro. purification of the inhibitory component present in s. pyogenes type m5 (manfredo strain) ce by anion-exchange chromatography followed by gel filtration chromatography showed that the inhibitor had an approximate native molecular mass of 100 kda. sodi ... | 1998 | 9632565 |
| a simple technique to isolate dna and supernatant of genital mycoplasma hominis and ureaplasma urealyticum. | vaginal colonization with mycoplasma hominis and ureaplasma urealyticum has been implicated as a cause of prematurity. several mechanisms to induce preterm labor have been discussed. the investigation of expressed and secreted enzymes requires a feasible method for culturing and further processing of these bacteria. we describe a simple technique for culturing of mycoplasma hominis and ureaplasma urealyticum without contamination with other microorganisms and isolating dna and supernatant. pcr a ... | 1998 | 9650134 |
| [infective endocarditis caused by unusual microorganisms]. | all series of infective endocarditis had a variable proportion of cases without an etiologic agent because all cultures were negative. new microbiologic techniques have permitted the discovery of the role of many microorganisms in infective endocarditis. c. burnetii is an increasing causative agent of subacute infective endocarditis. in the diagnosis, to the detection of antiphase-i antibodies, immunohistochemical, molecular techniques and cellular cultures have been added. total cure is difficu ... | 1998 | 9658953 |
| [catheter infection by mycoplasma hominis in a patient with acute lymphoblastic leukemia]. | 1998 | 9666596 | |
| the possible role of anaerobic bacteria in chronic prostatitis. | prostatis, the most common urological disease in men, afflicts between 25 and 50% of all adult men. four clinical categories are recognized: acute and chronic bacterial prostatitis, non-bacterial prostatitis and prostatodynia. the role of gram-positive aerobic bacteria and the different anaerobes in chronic bacterial prostatitis is still a matter of debate. during this study, the urethral discharge and the prostatic fluid obtained after prostatic massage of 50 patients with chronic prostatitis, ... | 1998 | 9669200 |
| chlamydia trachomatis: probable cause of prostatitis. | seventy-eight men with symptoms of chronic or subacute prostatitis were enrolled. investigations for the presence of chlamydia trachomatis in urethral swabs were carried out. the expressed prostatic secretions were additionally examined for mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, other gram-negative and gram-positive bacteria, trichomonas vaginalis, yeast-like fungi and leucocyte count. furthermore, all patients were evaluated for the presence of serum anti-chlamydial ... | 1998 | 9671250 |
| the in vitro simulation of mycoplasma hominis isolation using supplemented culture media in the bactec 9000 series blood culture system. | 1998 | 9684411 | |
| cloning and nucleotide sequences of the topoisomerase iv parc and pare genes of mycoplasma hominis. | the topoisomerase iv parc and pare genes from the wall-less organism mycoplasma hominis pg21 were cloned and sequenced. the coupled genes are located far from the dna gyrase genes gyra and gyrb. they encode proteins of 639 and 866 amino acids, respectively. as expected, the encoded pare and parc proteins exhibit higher homologies with the topoisomerase iv subunits of the gram-positive bacteria staphylococcus aureus and streptococcus pneumoniae than with their escherichia coli counterparts. the c ... | 1998 | 9687401 |
| the mycoplasma hominis vaa gene displays a mosaic gene structure. | mycoplasma hominis contains a variable adherence-associated (vaa) gene. to classify variants of the vaa genes, we examined 42 m. hominis isolated by pcr, dna sequencing and immunoblotting. this uncovered the existence of five gene categories. comparison of the gene types revealed a modular composition of the vaa proteins. the proteins constituted a conserved n-terminal part followed by a varying number of interchangeable cassettes encoding approximately 110 amino acids with conserved sequences b ... | 1998 | 9701806 |
| comparison of polymerase chain reaction assay with culture for detection of genital mycoplasmas in perinatal infections. | the polymerase chain reaction (pcr) technique was compared with culture for the detection of ureaplasma urealyticum, mycoplasma hominis, and mycoplasma genitalium in clinical samples (vaginal secretions, throat and endotracheal secretions, and skin swabs) obtained from 47 high-risk pregnant women peripartum and eight newborn infants. detection using pcr with homologous primers was highly specific, as a product with the expected length was consistently amplified with homologous but not with heter ... | 1998 | 9707308 |
| medical significance of mycoplasmas. | 1998 | 9711635 | |
| detection of mycoplasmas in urethral swabs from hiv-1 infected patients and control individuals using culture techniques and polymerase chain reaction. | the objective of the present study was to determine the prevalence of certain mycoplasma species, i.e., mycoplasma hominis, ureaplasma urealyticum and mycoplasma penetrans, in urethral swabs from hiv-1 infected patients compared to swabs from a control group. mycoplasmas were detected by routine culture techniques and by the polymerase chain reaction (pcr) technique, using 16srrna generic primers of conserved region and mycoplasma penetrans specific primers. the positivity rates obtained with th ... | 1998 | 9713129 |
| conservation of the amino-terminal epitope of elongation factor tu in eubacteria and archaea. | an epitope of elongation factor tu (ef-tu), which is found in organisms in both the bacterial and archaeal domains, was recently defined by mab 900. to localize the conserved epitope within the ef-tu molecule and to determine its sequence, spotscan analysis of synthetic peptides, western blot analysis of purified ef-tu domains and site-directed mutagenesis studies were used. analysis of mab 900 binding to overlapping 15-mer peptides encompassing the complete sequence of ef-tu of escherichia coli ... | 1998 | 9720046 |
| preterm birth: associations with genital and possibly oral microflora. | opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ... | 1998 | 9722706 |
| mycoplasma contamination of chlamydia pneumoniae isolates. | we examined 6 c. pneumonia isolates from the american type culture collection (atcc) and 2 finnish isolates for mycoplasma contamination. three of the atcc isolates and both of the finnish isolates were mycoplasma-contaminated. the contaminants were characterized by means of growth in bea and beg media, immunoblotting, polymerase chain reaction and pulsed field gel electrophoresis. two of the 6 atcc isolates [atcc vr1355 (twar strain 2043) and atcc vr1356 (twar strain 2023)] were infected with m ... | 1998 | 9730308 |
| dna sequencing reveals limited heterogeneity in the 16s rrna gene from the rrnb operon among five mycoplasma hominis isolates. | to investigate the intraspecies heterogeneity within the 16s rrna gene of mycoplasma hominis, five isolates with diverse antigenic profiles, variable/identical p120 hypervariable domains, and different 16s rrna gene rflp patterns were analysed. the 16s rrna gene from the rrnb operon was amplified by pcr and the pcr products were sequenced. three isolates had identical 16s rrna sequences and two isolates had sequences that differed from the others by only one nucleotide. | 1998 | 9734066 |
| alterations in topoisomerase iv and dna gyrase in quinolone-resistant mutants of mycoplasma hominis obtained in vitro. | mycoplasma hominis mutants were selected stepwise for resistance to ofloxacin and sparfloxacin, and their gyra, gyrb, parc, and pare quinolone resistance-determining regions were characterized. for ofloxacin, four rounds of selection yielded six first-, six second-, five third-, and two fourth-step mutants. the first-step mutants harbored a single asp426-->asn substitution in pare. gyra changes (ser83-->leu or trp) were found only from the third round of selection. with sparfloxacin, three round ... | 1998 | 9736554 |
| [detection using molecular biology techniques of mycoplasma hominis and ureaplasma urealyticum in urogenital samples]. | mycoplasma hominis and ureaplasma urealyticum are species closely related to urogenital diseases such as pyelonephritis, nongonococcal urethritis, urinary calculi, epididymitis, pelvic inflammation, infertility, abortions and post-delivery fever. they can also cause pneumonia and meningitis in newborn infants. in this paper we used nucleic acid hybridization and polymerase chain reaction to analyze 22 samples from patients with different urogenital symptoms in order to detect mycoplasmas and ure ... | 1998 | 9744030 |
| ultrasonographic assessment of the uterine cervix and interleukin-8 concentrations in cervical secretions predict intrauterine infection in patients with preterm labor and intact membranes. | interleukin-8 concentrations in cervical secretions have been related to microbial invasion of the amniotic cavity and histological chorioamnionitis. since a short uterine cervix may be a risk factor for intrauterine infection, we set out to determine the interrelationship between cervical-secretion interleukin-8 concentration, cervical length measured by transvaginal sonography and intrauterine infection in women with preterm labor and intact membranes. | 1998 | 9744050 |
| elevated amniotic fluid interleukin-6 as a predictor of neonatal periventricular leukomalacia and intraventricular hemorrhage. | > objective: to investigate the relationship between amniotic fluid interleukin-6 levels and the development of periventricular leukomalacia and intraventricular hemorrhage in the preterm neonate and to compare the value of amniotic fluid interleukin-6 with amniotic fluid culture and histologic chorioamnionitis in the prediction of periventricular leukomalacia and intraventricular hemorrhage. methods: 119 women, between 20 and 34 weeks gestation, in preterm labor with intact membranes, underwent ... | 1998 | 9745094 |
| absence of phospholipase a2 activity in genital mycoplasma hominis. | the clinical relevance of vaginal colonization with mycoplasma hominis (m hominis) as a cause of prematurity is doubtful. one of the possible pathways which could explain the role of m hominis in the induction of preterm labour is an increased synthesis of prostaglandins by a phospholipase a2 activity. the aim of this study was to prove whether m hominis secrets proteins with a pla2 activity and to test whether there are strain differences in the enzyme activity between m hominis isolated from w ... | 1998 | 9773381 |
| multiplex pcr for the detection of mycoplasma fermentans, m. hominis and m. penetrans in cell cultures and blood samples of patients with chronic fatigue syndrome. | a multiplex polymerase chain reaction (pcr) was initially developed to detect the presence of mycoplasma genus dna sequences in cell cultures and to differentiate between three human pathogenic mycoplasma species simultaneously. the assay in turn, proved to be a useful tool for the detection of mycoplasma infection in human dna samples. one set of oligonucleotide primers which are specific for a highly conserved region among all members of the genus mycoplasma along with three other primer sets ... | 1998 | 9778455 |
| mycoplasma hominis parasitism of trichomonas vaginalis. | 1998 | 9788469 | |
| dna analysis for phospholipase a2 coding sequences of mycoplasma hominis isolated from women with a normal pregnancy and women with a pregnancy complicated by preterm labour. | a possible cause of preterm labour is an increased synthesis of prostaglandins by a phospholipase a2 (pla2) activity. pla2 activity has been detected in mycoplasma hominis. the aim of this study was to test whether chromosomal dna of m. hominis contains sequences coding for pla2. m. hominis was cultured in specimens from 5 women with normal pregnancy and 4 in preterm labour. using sequence alignment, primer pairs for the active part of pla2 of different species were designed for pcr analysis. no ... | 1998 | 9789649 |
| cloning and expression of p60, a conserved surface-localized protein of mycoplasma hominis, in escherichia coli. | the clp60 gene encoding p60, a conserved lipoprotein of mycoplasma hominis, was cloned and sequenced from both the type strain pg21 and the isolate fbg. both open reading frames were identical in length, comprising 1746 nucleotides. the deduced amino acid sequences differed in 16 out of 582 amino acids. as expected, none of these divergences mapped within the epitope that was recognized by mab cg4 in all of the 198 isolates of m. hominis analyzed so far. this conserved epitope was narrowed down ... | 1998 | 9792448 |
| genital mycoplasmas stimulate tumor necrosis factor-alpha and inducible nitric oxide synthase production from a murine macrophage cell line. | ureaplasma urealyticum and mycoplasma hominis, two genital mycoplasmas, are the most common organisms isolated in the perinatal period and both either cause or are associated with poor perinatal outcomes. we speculate that these microbes could increase inflammation by stimulating macrophages to produce tumor necrosis factor-alpha (tnf-alpha) and inducible nitric oxide synthase because of their propensity to interact with the host's immune system. to test this hypothesis, raw 264.7 cells, a murin ... | 1998 | 9803463 |
| exogenous bovine surfactant suppresses tumor necrosis factor-alpha release by murine macrophages stimulated by genital mycoplasmas. | tumor necrosis factor-alpha (tnf-alpha) is a proinflammatory cytokine that appears to play a significant role in the development of neonatal chronic lung disease (cld). inflammation and cld are also associated with respiratory tract colonization with genital mycoplasmas. the possible protective roles of surfactant in mitigating the inflammatory response to these microbes were investigated. murine raw 264.7 macrophages were preincubated with an exogenous surfactant and exposed overnight to steril ... | 1998 | 9806043 |
| [detection and analysis of mycoplasma in 400 cases of genitourinary infection in wuhan area]. | mycoplasmas were examined by cultured in the urogenital secreta and the midpieceurine from 400 cases (88 men and 312 women) of genitourinary infection and 119 cases (positive rate 29.75%) of genitourinary infection in wuhan area. the positive rates of ureaplasma urealyticum (uu) and mycoplasma hominis (mh) were 24.75% and 8.75% respectively. the authors also studied the infection rates of neisseria gonorrheae, fungus and trichomonad by cultures and microscopic findings. the positive rates of nei ... | 1997 | 9812505 |
| rubella virus and chronic joint disease: is there an association? | synovial fluid samples and/or biopsies from 79 patients with various chronic inflammatory joint diseases or traumatic joint injury were tested for rubella virus (rv) in order to confirm or refute results from other studies that suggested rv as a cause of chronic inflammatory joint disease. sixty-eight of the 72 patients tested had rv antibodies. rv rna was detected by reverse transcription-pcr in the synovial fluid cells from two patients. rv was also isolated by cell culture from the synovial f ... | 1998 | 9817866 |
| [formation of m. hominis and a. laidlawii resistance to fluoroquinolones]. | mycoplasma hominis and acholeplasma laidlawii cultures resistant to antibacterial fluoroquinolone drugs ciprofloxacin (cpf), ofloxacin (ofl), and lomefloxacin (lmf) were prepared by selection in liquid nutrient medium with ascending concentrations of cpf. resistant mycoplasma clones contained point mutations in the gyrase. a gene region determining quinolone resistance (qrdr gyra): m. hominis contained c-->t transition resulting in substitution of ser(83) for leu and a. laidlawii g-->a resulting ... | 1998 | 9819821 |
| complications of abortion performed under local anesthesia. | to assess the incidence of complications of abortion performed under local anesthesia. | 1998 | 9846716 |
| truncation as a novel form of variation of the p50 gene in mycoplasma hominis. | a characteristic feature of the mycoplasmas is the presence of variable surface proteins which may play an important role in the adaptation of the cell-wall-less organisms to their host environments. in addition, this antigen variation may be an important pathogenic property of the organism. the ubiquity of the gene encoding p50, an adhesin of mycoplasma hominis fbg, and its transcription were analysed in different isolates of m. hominis. the p50 gene was present in all isolates tested. based on ... | 1998 | 9846733 |
| association of genital mycoplasma colonization with low birth weight. | this study was conducted to document the prevalence of maternal genital tract colonization by ureaplasma urealyticum and mycoplasma hominis, and to assess its association with low birth weight (lbw) and prematurity. | 1998 | 9856315 |
| mycoplasma hominis parasitism of trichomonas vaginalis. | 1998 | 9872279 | |
| antigenic and genomic homogeneity of successive mycoplasma hominis isolates. | sixty mycoplasma hominis isolates were obtained from the cervices of pregnant women and from the ears or pharynges of their newborn babies. the isolates were examined by sds-page and pulsed-field gel electrophoresis. antigenic and genomic profiles were obtained for 16 series with two or more successive isolates. both analyses led to the conclusion that isolates from the same woman were identical or nearly identical, while isolates from different women exhibited a high degree of variation with re ... | 1998 | 9877186 |
| two patients with premature labour associated with mycoplasma hominis infection. | because several reports have suggested that bacterial vaginosis causes premature labour and early rupture of the fetal membranes, the presence of a bacterial flora that causes bacterial vaginosis is thought to be a risk factor for premature labour. the present study investigated two patients with premature delivery and intra-uterine mycoplasma hominis infection. in microbiological studies, gram's staining of amniotic fluids revealed numerous neutrophils and epithelial cells but no micro-organism ... | 1998 | 9879962 |
| [rokitamycin in the treatment of female genital chlamydia and mycoplasma infections. comparative study vs josamycin ]. | the macrolides are among the most effective antibiotics against infections due to chlamydia and mycoplasma. the drug in such cases must have marked antibacterial activity, good oral bioavailability, and high intracellular diffusion--indispensable for instance with chlamydia infection. rokitamycin, a macrolide with a 16-atom lactone ring, has the features for use in the treatment of genital infections caused by chlamydia or mycoplasma, penetrating the cell and reaching considerably higher concent ... | 1998 | 9882991 |
| ureaplasma urealyticum and mycoplasma hominis isolation from cervical secretions of pregnant women and nasopharyngeal secretions of their babies at delivery. | a prospective study was carried out at the university hospital, kuala lumpur to determine the cervical carriage rate of ureaplasma urealyticum and mycoplasma hominis among healthy pregnant women at delivery and the incidence of nasopharyngeal colonisation among their infants. | 1998 | 9885690 |
| [survey of sexually transmitted diseases in the region of rio cuarto]. | sexually transmitted diseases (std) are acquired mainly through sexual intercourse, being one of the most frequent groups of infectious diseases worldwide and consequently an important public health problem. the aim of this paper was to determine the current state of std and to compare different diagnostic methods in the population studied. a total of 1060 samples from vaginal flows, endocervical material and urethral discharge were studied during 3 years. of the total samples, 583 were positive ... | 1998 | 9922478 |
| isolation of ureaplasma urealyticum and mycoplasma hominis from public toilet bowls. | the presence of ureaplasma urealyticum, mycoplasma hominis, and chlamydia trachomatis was explored in 50 public restroom toilet bowls. we used culture, antigen detection, polymerase chain reaction, and survival assay. five bowls (10%) were contaminated with at least one organism. u. urealyticum was found in four bowls, m. hominis in three, and c. trachomatis in one. u. urealyticum survived on the toilet rim for up to 2 hours. | 1999 | 9927273 |
| [detection of tetracycline- and erythromycin-resistant urogenital mycoplasma strains using pcr]. | the use of pcr made it possible to obtain data on the occurrence of genital mycoplasms in subjects with different pathological states of their urogenital system. the detection rate of u.urealyticum was 10.7-25.1% in males and 23.0-61.9% in females. the detection rate of mycoplasma hominis was 6.2-11.4% in males and 3.8-22.9% in females. the detection rate of m.genitalium was 6.2-29.6% in males and 4.7-30.0% in females. the study carried out by means of amplification test systems for the detectio ... | 1998 | 9949490 |
| semen culture, leukocytospermia, and the presence of sperm antibodies in seminal hyperviscosity. | seminal hyperviscosity is generally thought to reveal genitourinary infection. the aim of the present work was to study this hypothesis. a total of 65 semen samples were obtained from males presenting for infertility screening. the samples were evaluated according to who criteria and microbiologically investigated, including culturing for mycoplasma hominis and ureaplasma urealyticum, and microscopic observation of chlamydia trachomatis by a direct fluorescence assay. determination of local anti ... | 1999 | 9973141 |
| simultaneous detection of hpv and other sexually transmitted agents in chronic urethritis. | many pathogens may be responsible of non gonococcal urethritis (ngu) with the possible occurrence of symptomatic and asymptomatic mixed viral and bacterial infections. in particular, genital papillomaviruses (hpvs) have been searched since they are linked to both benign and malignant lesions of the penis and urethra and the presence of a potential male carried state has received limited scrutiny while the screening of sexually active females has received substantial attention. | 1998 | 9973807 |
| [use of a method of single-stranded dna fragment conformation polymorphism (sscp) for detecting resistance of mycoplasm hominis to fluoroquinolones]. | single-strand conformation polymorphism (sscp) method was used for nucleotide sequence variation analysis of the gyrase a subunit quinolone resistance determining region (gyra qrdr) in a laboratory and clinical strains of m. hominis. the couple of primers selected for this region amplified specific product in clinical material. m. hominis cultures growing in the presence of different concentrations of ciprofloxacin were studied by the sscp method. ser(83) to leu mutation described previously was ... | 1998 | 9987752 |
| vaginal heat shock protein expression in symptom-free women with a history of recurrent vulvovaginitis. | the cause of recurrent vulvovaginitis remains unexplained in most cases. heat shock protein synthesis is induced under conditions of stress; its presence in vaginal samples from women who were between episodes of recurrent vulvovaginitis thus might reflect a persistent perturbation in the local milieu. study design: we undertook an evaluation by means of enzyme-linked immunosorbent assay of 60-kd heat shock protein and inducible 70-kd heat shock protein expressions in vaginal wash samples from 2 ... | 1999 | 10076122 |
| intravenous azithromycin. | to review the pharmacology, microbiology, chemistry, pharmacokinetics, efficacy, safety, tolerability, dosage, administration, and economic issues of intravenous azithromycin. | 1999 | 10084418 |
| [acute cystitis]. | 1999 | 10088462 | |
| mutations in the gyra, parc, and pare genes associated with fluoroquinolone resistance in clinical isolates of mycoplasma hominis. | five clinical isolates of mycoplasma hominis from three different patients were examined for resistance to fluoroquinolones; some of these isolates were probably identical. all five isolates harbored amino acid substitutions in the quinolone resistance-determining regions of both dna gyrase (gyra) and topoisomerase iv (parc or pare). furthermore, the novobiocin mic for three isolates showed a significant increase. this is the first characterization of fluoroquinolone-resistant clinical mycoplasm ... | 1999 | 10103208 |
| [mycoplasma hominis and ureaplasma urealyticum in different gynecologic diseases]. | the incidence of mycoplasma hominis (m. hominis) y ureaplasma urealyticum (u. urealyticum) was investigated in 113 endocervical samples obtained from women who were seen for different gynecological pathologies. forty-seven (42%) patients were positive to these microorganisms; 26 cases (23%) were positive for m. hominis and 21 (19%; p = ns) for u. urealyticum. average age was 32.1 +/- 7.7 years; the average number of sexual partners was 1.7 +/- 1.1. eleven of 17 patients with 3 o more sexual part ... | 1999 | 10198558 |
| mycoplasma penetrans and other mycoplasmas in urine of human immunodeficiency virus-positive children. | urine samples from children with human immunodeficiency virus (hiv) infection and healthy controls were examined for mycoplasmas by culture. standard biochemical assays, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and pcr (16s and 16s-23s spacer rrna region) were used for identification of isolates. mycoplasmas were identified from 13 (87%) of 15 hiv-positive patients and 3 (20%) of 15 hiv-negative control patients. the frequency and type of mycoplasma varied with the severity of ... | 1999 | 10203515 |
| the characterization of mycoplasma synoviae ef-tu protein and proteins involved in hemadherence and their n-terminal amino acid sequences. | an abundant cytoplasmic 43-kda protein from mycoplasma synoviae, a major pathogen from poultry, was identified as elongation factor tu. the n-terminal amino acid sequence (akldfdrskehvnvgtighv) has 90% identity with the sequence of the mycoplasma hominis elongation factor tu protein. monoclonal antibodies reacting with the m. synoviae elongation factor tu protein also reacted with 43-kda proteins from the avian mycoplasma species mycoplasma gallinarum, mycoplasma gallinaceum, mycoplasma pullorum ... | 1999 | 10220885 |
| [mycoplasma hominis and ureaplasma urealyticum of cervical canal and in the pouch of douglas in infertile women: a preliminary report]. | detection of mycoplasma hominis and ureaplasma urealyticum in the samples obtained from the cervical canal of the uterus and from the pouch of douglas in 71 women in whom underwent diagnostic and operative laparoscopy is presented. ureaplasma urealyticum in 33 cases diagnosed for infertility was present in 6 (18.2%) patients. in cervical canal and the pouch of douglas in 5 and 2 women respectively. in 1 (3.0%) woman ureaplasma urealyticum was present in both places. in the women from the control ... | 1998 | 10224793 |
| cyto-adherence studies of the adhesin p50 of mycoplasma hominis. | recombinant peptides of mycoplasma hominis adhesin p50 were expressed in escherichia coli to investigate which regions of the p50 molecule are responsible for cyto-adherence. the respective dna fragments were obtained by pcr amplification of the p50 gene with the use of mutating oligonucleotides to change the tga codons of mycoplasma to tgg codons, which are translated in e. coli as tryptophan. the resulting three clones (i, i+ii and i+iii) contained regions of p50 which closely represent the re ... | 1999 | 10229546 |
| [pathogen spectrum of urethritis in the man]. | 545 male patients with a tentative diagnosis "urethritis" were examined between november 1984 and december 1994 in the department of dermatology and venerology of the military hospital in ulm. the patients, aged from 18 to 58 years (mean age 24.1 years), were examined according to a standardized diagnostic procedure: smear preparations from the urethra with subsequent gram staining, bacterial cultures for aerobic bacteria, neisseria gonorrhoeae (cultures and phadebact gonococcus test), mycoplasm ... | 1999 | 10231688 |
| a comparative evaluation of vaginal, cervical and peritoneal flora in normal, healthy women: a preliminary report. | twelve healthy women undergoing laparoscopic tubal sterilization were studied. specimens were obtained for culture of aerobic and anaerobic bacteria, mycoplasma hominis, ureaplasma urealyticum, and chlamydia trachomatis. the sites cultured included cul-de-sac (through a laparoscope), cul-de-sac (by transvaginal culdocentesis), the vaginal wall, and the endocervical canal. anaerobic bacteria were isolated from the peritoneal cavity of three (25%) of the subjects. these isolates included b. melani ... | 1982 | 10328022 |
| infection of a traumatic pelvic hematoma with mycoplasma hominis. | fever developed in a previously healthy young man who had sustained extensive pelvic trauma. mycoplasma hominis was isolated in pure culture from six of seven specimens taken from a retroperitoneal hematoma over a one-week period, and mycoplasmacidal antibodies were present in high titer in the convalescent-phase serum. the fever abated after thorough surgical drainage of the infected hematoma. | 1978 | 10328034 |
| evaluation of the etest for susceptibility testing of mycoplasma hominis and ureaplasma urealyticum. | the etest was used for antibiotic susceptibility testing of mycoplasma hominis and ureaplasma urealyticum isolates and the results were compared with those obtained with the broth microdilution method. for 50 clinical isolates of m. hominis the mics of doxycycline, ofloxacin and ciprofloxacin agreed within +/- one dilution and +/- two dilutions in 82-98% and 98-100% of cases, respectively. the mics of erythromycin, azithromycin, doxycycline, ofloxacin and ciprofloxacin were evaluated for 50 clin ... | 1999 | 10350390 |
| isolation pattern and clinical outcome of genital mycoplasma in neonates from a tertiary care neonatal unit. | the role of genital mycoplasma in perinatal mortality and morbidity has been debated. this study was undertaken to determine the frequency of isolation of genital mycoplasma and evaluate its association with clinical outcome. sixty-six cerebrospinal fluid (csf) and 49 tracheal aspirates taken from 100 low birthweight infants who had suspected meningitis and/or respiratory distress respectively were cultured for genital mycoplasma. ureaplasma urealyticum was isolated from 9 per cent of csf and 14 ... | 1999 | 10401191 |
| comparative in-vitro activity of levofloxacin, other fluoroquinolones, doxycycline and erythromycin against ureaplasma urealyticum and mycoplasma hominis. | the susceptibility of 56 ureaplasma urealyticum and 57 mycoplasma hominis strains to levofloxacin, ofloxacin, ciprofloxacin, fleroxacin, doxycycline and erythromycin was determined by an agar dilution method. the reference strain used was m. hominis pg 21. agar plates containing serial dilutions of antibiotics (range 0.03-16 mg/l), and control plates (without antibiotics) were inoculated with bacteria suspended in modified shepard's broth using a multipoint inoculator. levofloxacin showed greate ... | 1999 | 10404335 |
| [genetic variability of mycoplasma during interaction with host: vertical transfer and rearrangement in genes of surface antigenic determinants]. | 1999 | 10420256 | |
| detection of viral and bacterial infections in women with normal and abnormal colposcopy. | signs and symptoms of sexually-transmitted diseases (std) do not allow any etiological diagnosis in women. colposcopic findings are seldom pathognomic. consequently, the microbiology laboratory with the recent availability of molecular diagnostic tools is required to detect the infectious bacterial and/or viral agents involved in std. in cervical samples of women submitted to gynaecological screening for past or present signs and symptoms of inflammation and with different colposcopic findings, ... | 1999 | 10422688 |
| relationship between ureaplasma urealyticum vaginal colonization and polymorphism in the interleukin-1 receptor antagonist gene. | the relationship between polymorphisms in the interleukin-1 receptor antagonist (il-1ra) gene and microbial vaginal colonization was examined in 88 asymptomatic women of reproductive age. alleles of the intron 2 region of the il-1ra gene were identified by polymerase chain reaction (pcr). pcr was also used to detect ureaplasma urealyticum, mycoplasma hominis, and candida albicans; bacterial vaginosis (bv) was identified by clinical criteria. among the 31 women with vaginal u. urealyticum, only 3 ... | 1999 | 10438393 |
| the adherence-associated lipoprotein p100, encoded by an opp operon structure, functions as the oligopeptide-binding domain oppa of a putative oligopeptide transport system in mycoplasma hominis. | mycoplasma hominis, a cell-wall-less prokaryote, was shown to be cytoadherent by the participation of a 100-kda membrane protein (p100). to identify the gene encoding p100, peptides of p100 were partially sequenced to enable the synthesis of p100-specific oligonucleotides suitable as probes for the detection of the p100 gene. with this strategy, we identified a genomic region of about 10. 4 kb in m. hominis fbg carrying the p100 gene. analysis of the complete deduced protein sequence suggests th ... | 1999 | 10438757 |
| scanning electron microscopy of endometrial biopsies of patients with bacterial vaginosis shows morphology resembling mycoplasma/ureaplasma organisms. | 1999 | 10448406 | |
| mycoplasma hominis bacteraemia not associated with genital infections. | we describe two cases of systemic infection with m. hominis without prior genital infection and review the previous literature on this condition. | 1999 | 10468136 |
| [clinicobacteriological studies on the nine cases with upper genital tract mycoplasma hominis infection]. | clinicobacteriological characteristics of nine cases isolated mycoplasma hominis from the genital tract were studied, and the following results were obtained: elevation of igg antibodies to m. hominis was measured by elisa in all cases, but in the mi method only one case showed an elevation of metabolic inhibitory antibody. convalescent sera from seven patients showed additional and high density bands which were not recognized by acute phase sera in immunoblotting. it was thought that in two pat ... | 1999 | 10481399 |
| amplified-fragment length polymorphism fingerprinting of mycoplasma species. | amplified-fragment length polymorphism (aflp) is a whole-genome fingerprinting method based on selective amplification of restriction fragments. the potential of the method for the characterization of mycoplasmas was investigated in a total of 50 strains of human and animal origin, including mycoplasma genitalium (n = 11), mycoplasma pneumoniae (n = 5), mycoplasma hominis (n = 5), mycoplasma hyopneumoniae (n = 9), myco plasma flocculare (n = 5), mycoplasma hyosynoviae (n = 10), and mycoplasma di ... | 1999 | 10488196 |
| sparfloxacin selects gyrase mutations in first-step mycoplasma hominis mutants, whereas ofloxacin selects topoisomerase iv mutations. | the role of mutations in the genes for gyra and parc in quinolone resistance in mycoplasma hominis was studied. selection with sparfloxacin gave mutations at gyra83 (ser-->leu; escherichia coli numbering) or gyra87 (glu-->lys), and mutants had increased levels of resistance to sparfloxacin (8- to 16-fold) but not to ofloxacin. selection with ofloxacin gave changes at parc80 (ser-->ile) or parc84 (glu-->lys), and mutants were four- to eightfold more resistant to ofloxacin but not to sparfloxacin. ... | 1999 | 10508030 |
| in vitro susceptibilities of mycoplasma hominis to six fluoroquinolones as determined by e test. | twenty isolates of mycoplasma hominis were tested for their susceptibility to six fluoroquinolones by the e test. the mics at which 90% of the isolates were inhibited (in micrograms per milliliter) were as follows: sparfloxacin, 0.031; clinafloxacin, moxifloxacin, and trovafloxacin, 0.063; levofloxacin, 0.25; and ciprofloxacin, 0.5. increasing the amount of inoculum or incubation in co(2) elevated mics by </=1 dilution. e tests produce fluoroquinolone mics comparable to those obtained by agar an ... | 1999 | 10508049 |
| association of indicators of bacterial vaginosis with a female genital tract factor that induces expression of hiv-1. | the aim of this study was to determine the relationship of bacterial vaginosis and bacterial vaginosis-associated microorganisms with an hiv-inducing factor (hif) found in cervicovaginal lavage. | 1999 | 10513649 |
| a double-blind, randomized, controlled multicentre study to compare the efficacy of ciprofloxacin with pivampicillin as oral therapy for epididymitis in men over 40 years of age. | to compare the efficacy and safety of ciprofloxacin 500 mg orally twice daily with pivampicillin 700 mg orally twice daily for 10 days in men with acute epididymitis and over 40 years of age. | 1999 | 10532980 |
| evaluation of igg, igm, and iga antibodies to mycoplasma penetrans detected by elisa and immunoblot in hiv-1-infected and std patients, in são paulo, prazil. | the aim of the present study was to determine the frequency of igg, iga, and igm antibodies to mycoplasma penetrans in hiv-1-infected patients and in patients with sexually transmitted diseases. we tested serum samples from 106 hiv-1-positive patients and 110 individuals with clinical symptoms of urethritis. elisa and the immunoblot test were performed using m. penetrans lipid associated membrane proteins as antigen. by elisa, we found a higher frequency (p < 0.05) of igg against m. penetrans in ... | 1999 | 10572313 |
| life-threatening mycoplasma hominis mediastinitis. | mycoplasma hominis infections are easily missed because conventional methods for bacterial detection may fail. here, 8 cases of septic mediastinitis due to m. hominis are reported and reviewed in the context of previously reported cases of mediastinitis, sternum wound infection, pleuritis, or pericarditis caused by m. hominis. all 8 patients had a predisposing initial condition related to poor cardiorespiratory function, aspiration, or complications related to coronary artery surgery or other th ... | 1999 | 10585808 |
| mycoplasmal infections alter gene expression in cultured human prostatic and cervical epithelial cells. | to better understand how infections by mycoplasmas affect gene expression in human cells, we quantitatively measured the transcripts of 38 cytokine genes in hpv e6- and e7-immortalized cervical and prostatic epithelial cells before and after infection by four human urogenital mycoplasmas, m. fermentans, m. genitalium, m. hominis and m. penetrans. using the multi-probe rnase protection assay (rpa), 22 and 23 cytokine gene transcripts were detected in the non-infected control prostatic and cervica ... | 2000 | 10617789 |
| [role of mutations in parc and gyra in forming resistance of mycoplasma hominis to fluoroquinolones]. | the set of the laboratory strain m. hominis h-34 mutants resistant to fluoroquinolones (ciprofloxacin-cfl, lomefloxacin-lfl, ofloxacin-ofl) was obtained by selection in broth medium. the mutation was found in the quinolone resistance-determining region (qrdr) of a subunit of topoisomerase iv gene (parc) and new mutations were found in qrdr of genes encoding the a subunit of dna gyrase (gyra) in m. hominis mutants resistant to various concentrations of the cfl, lfl and ofl. after multistep select ... | 1999 | 10621934 |
| [association of tap1/tap2 gene polymorphism with inflammatory urogenital diseases combined with joint diseases]. | to study association of tap1/tap2 gene polymorphism with urogenital infections combined with joint lesions. | 1999 | 10626334 |
| accurate detection of male subclinical genital tract infection via cervical culture and dna hybridization assay of the female partner. | the accuracy of the pace2 dna hybridization assay of the cervix and cervical culture in female partners for the diagnosis of male subclinical genital tract infection were assessed in a male infertility population. a total of 184 men were screened for the presence of chlamydia trachomatis, ureaplasma urealyticum and mycoplasma hominis. seventy-one men were identified with a positive test for one or more of the above mentioned micro-organisms. the overall prevalence of bacterial infection was 39%. ... | 2000 | 10632761 |
| cloning, sequencing and variability analysis of the gap gene from mycoplasma hominis. | the gap gene encodes the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (gapdh). the gene was cloned and sequenced from the mycoplasma hominis type strain pg21(t). the intraspecies variability was investigated by inspection of restriction fragment length polymorphism (rflp) patterns after polymerase chain reaction (pcr) amplification of the gap gene from 15 strains and furthermore by sequencing of part of the gene in eight strains. the m. hominis gap gene was found to vary more than ... | 2000 | 10650196 |
| effect of a mycoplasma hominis-like mycoplasma on the infection of hep-2 cells by the tw-183 strain of chlamydia pneumoniae. | we isolated a mycoplasma hominis-like mycoplasma from a stock culture of chlamydia pneumoniae tw-183 obtained from the american type culture collection and eradicated the contaminant by treating the stock suspension with a nonionic detergent, igepal ca-630. the m. hominis-like mycoplasma neither inhibits nor enhances the infectivity of c. pneumoniae for hep-2 cells. | 2000 | 10655399 |
| detection of microorganisms in the tracheal aspirates of preterm infants by polymerase chain reaction: association of adenovirus infection with bronchopulmonary dysplasia. | bronchopulmonary dysplasia (bpd) is recognized as an important cause of morbidity and mortality in preterm infants. because the role of congenital infections in bpd has been debated, the purpose of this study was to test the hypothesis that detection of infectious agents in tracheal aspirate samples was associated with the development of bpd. tracheal aspirate samples were obtained within the 1st week of life and screened by polymerase chain reaction for adenovirus, cytomegalovirus, parvovirus, ... | 2000 | 10674351 |
| [investigation of intrauterine microbes after intrauterine operation]. | to explore the postoperative changes in the cultures of ureaplasma urealyticum (uu), mycoplasma hominis (mh), l-form bacteria (l-form), anaerobic bacteria (ana) and chlamydia trochomatis (ct) after intrauterine operation. | 1998 | 10682488 |
| ureaplasma/mycoplasma-infected amniotic fluid: pregnancy outcome in treated and nontreated patients. | to determine if treatment of a positive amniotic fluid culture for mycoplasmal colonization obtained at genetic amniocentesis is associated with improved pregnancy outcome. | 1999 | 10685238 |
| multiple mycoplasmal infections detected in blood of patients with chronic fatigue syndrome and/or fibromyalgia syndrome. | the aim of this study was to investigate the presence of different mycoplasmal species in blood samples from patients with chronic fatigue syndrome and/or fibromyalgia syndrome. previously, more than 60% of patients with chronic fatigue syndrome/fibromyalgia syndrome were found to have mycoplasmal blood infections, such as mycoplasma fermentans infection. in this study, patients with chronic fatigue syndrome/fibromyalgia syndrome were examined for multiple mycoplasmal infections in their blood. ... | 1999 | 10691196 |
| retroperitoneal abscess and bacteremia due to mycoplasma hominis in a polytraumatized man. | we report a case of a retroperitoneal abscess due to mycoplasma hominis in a young polytraumatized man who developed septicemia under treatment with rifampin and flucloxacillin. m. hominis was recovered from blood cultures as well as from the abscess near the left iliac spine. after 10 days of therapy with clindamycin the patient improved, and intraoperatively taken swabs were culture negative but still positive by pcr. | 2000 | 10697792 |
| in vitro activity of gemifloxacin (sb 265805; lb20304a) against human mycoplasmas. | the in vitro activity of gemifloxacin, a new broad-spectrum fluoroquinolone, was compared with those of ciprofloxacin, erythromycin, azithromycin and doxycycline against 29 human respiratory or urogenital tract mycoplasmas. gemifloxacin was highly active against all of the mycoplasma and ureaplasma species tested (mic range 0.001-0.25 mg/l) and was 5- to 100-fold more active than ciprofloxacin. doxycycline was less active than gemifloxacin against the mycoplasmas (mic range 0.01-1 mg/l) but had ... | 2000 | 10702559 |
| molecular comparison of mycoplasma hominis strains isolated from colonized women and women with various urogenital infections. | twenty mycoplasma hominis strains isolated from colonized women and women with various urogenital infections were investigated for genetic and antigenic homogeneity by different methods. restriction fragment length polymorphism analysis demonstrated heterogeneity for all strains, with one exception. two strains sequentially isolated from one patient showed identical patterns. otherwise, no clonal clustering could be detected within the strains isolated from either of the diagnostic groups. in co ... | 2000 | 10705620 |
| molecular dissection of mycoplasma hominis. | m. hominis is commonly found as part of the normal flora in the female genital tract, but several studies have shown that it may be involved in a variety of urogenital infections. the basis for clinical manifestations in some patients has varyingly been attributed to host and m. hominis factors. the host factors involved in the infection process are largely unknown. m. hominis have no cell wall and outer membranes, and at present it seems plausible that m. hominis possesses genetic systems allow ... | 2000 | 10721331 |
| [the etiology of infectious cervicitis in women]. | infectious cervicitis is a common disease in women of reproductive age. a prospective study was carried out including 70 women with clinical manifestations of cervicitis during gynecological and colposcopic examination. in 19 women (27%), chlamydia trachomatis was demonstrated by direct immunofluorescence in cervical smears. neisseria gonorrhoeae was isolated in 2 women and 1 had a concomitant infection with neisseria gonorrhoeae and chlamydia trachomatis. mycoplasma hominis and ureaplasma ureal ... | 1999 | 10730379 |
| association of chronic urinary symptoms in women and ureaplasma urealyticum. | objectives. to determine the incidence of ureaplasma urealyticum in women experiencing chronic urinary symptoms and to determine whether antibiotic therapy targeting these organisms is effective. | 2000 | 10736488 |
| genetic basis of natural resistance to erythromycin in mycoplasma hominis. | 2000 | 10747837 | |
| [the use of in situ polymerase chain reaction for detection the intracellular localization of mycoplasma hominis in cultured hela cells]. | mycoplasma hominis was incubated with hela cells up to 72 h. infection of hela cells was estimated by in situ polymerase chain reaction with a subsequent use of computer digital image analysis for result processing. the localization of mycoplasma in the cell nucleus was demonstrated after a 48 h incubation. the quantity of infected hela cells was increased with time to make 58 and 75% on days 2 and 3, respectively. no m. hominis was found in the cytoplasma of hela cells. | 2000 | 10752127 |
| effect of some psychotropic drugs and a barbiturate on mycoplasmas. | the inhibitory effect of selected membrane stabilisers on mycoplasma pneumoniae, m. hominis and ureaplasma urealyticum was investigated in vitro. the phenothiazine chlorpromazine (cpz) and the barbiturate thiopental (leopental(r)) as well as the stereo-isomeric thioxanthene derivatives; cis(z)-clopenthixol (zu-clopenthixol(r))/ trans (e)-clopenthixol and cis (z)-chlorprothixen (truxal(r))/trans(e)-chlorprothixen, all have antimycoplasmal effect in the range 3.9-312 mg/l, measured as growth inhib ... | 2000 | 10773494 |
| [in vitro sensitivity to antibiotics in 178 strains of genital mycoplasma isolated from gynecology consultants in dakar]. | the susceptibility to antibiotics of 144 strains of ureaplasma urealyticum and 34 strains of mycoplasma hominis isolated in dakar, senegal, was determinated by mic determination in a medium. doxycyclin and minocyclin are active on more than 90% of the strains of u. urealyticum, and more than 80% of m. hominis strains. over 93% of u. urealyticum strains are susceptible to all the macrolids and apparented tested (erythromycin, pristinamycin, josamycin), but the activity of lincomycin, pristinamyci ... | 2000 | 10774483 |