Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| time to manage mycoplasma genitalium as an sti: but not with azithromycin 1 g! | mycoplasma genitalium is a sexually transmitted infection that causes significant morbidity in men and women and is a co-factor in hiv transmission. however, commercial diagnostic tests are not generally available for m. genitalium and sub-optimal treatment is often given. we review the literature on the burden of infection, how it may present in clinical practice and the effectiveness of current treatment regimens. | 2014 | 24322592 |
| [leading role of infection in formation of placental insufficiency]. | evaluate the role of endotoxinemia in pregnancy complicated with placental insufficiency. | 2013 | 24341208 |
| functional characterization of osmotically inducible protein c (mg_427) from mycoplasma genitalium. | mycoplasma genitalium is the smallest self-replicating bacterium and an important human pathogen responsible for a range of urogenital infections and pathologies. due to its limited genome size, many genes conserved in other bacteria are missing in m. genitalium. genes encoding catalase and superoxide dismutase are absent, and how this pathogen overcomes oxidative stress remains poorly understood. in this study, we characterized mg_427, a homolog of the conserved osmc, which encodes hydroperoxid ... | 2014 | 24363346 |
| evaluation of two commercial real-time pcr assays for detection of mycoplasma genitalium in urogenital specimens. | the performance of two commercial real-time pcr kits for the detection of mycoplasma genitalium was evaluated in comparison to an in-house real-time pcr assay. concordances of 96% and 93% were found for the tib molbiol and the diagenode assays, respectively, compared to the results of the in-house assay. | 2014 | 24371239 |
| extensive variation and rapid shift of the mg192 sequence in mycoplasma genitalium strains from patients with chronic infection. | mycoplasma genitalium causes persistent urogenital tract infection in humans. antigenic variation of the protein encoded by the mg192 gene has been proposed as one of the mechanisms for persistence. the aims of this study were to determine mg192 sequence variation in patients with chronic m. genitalium infection and to analyze the sequence structural features of the mg192 gene and its encoded protein. urogenital specimens were obtained from 13 patients who were followed for 10 days to 14 months. ... | 2014 | 24396043 |
| co-infection of mycoplasma genitalium and chlamydia trachomatis in an infertile female patient with genital tuberculosis. | genital tuberculosis is a common cause of female infertility in india. but, it is important to screen for other agents like chlamydia trachomatis and genital mycoplasmas as well to avoid persistence of infection and its long-term sequelae. timely diagnosis of these infections using nucleic acid amplification tests and institution of appropriate therapy will improve the conception rates in infertile women. we report a case of co-infection of mycoplasma genitalium and chlamydia trachomatis in an i ... | 2014 | 24441248 |
| an epidemiological study of mycoplasma genitalium infections among males attending a sexually transmitted disease clinic in guangxi, china. | the objective of this study was to determine the prevalence of urethral mycoplasma genitalium infections among male patients attending a sexually transmitted disease (std) clinic in china and identify risk factors associated with this disease. a total of 423 patients were recruited in hezhou city, guangxi province, china, and each was requested to complete a questionnaire regarding sociological and sexual behaviors. first-void urine samples were collected for m. genitalium analysis by polymerase ... | 2014 | 24451096 |
| prevalence of sexually transmitted infections among healthy korean women: implications of multiplex pcr pathogen detection on antibiotic therapy. | in this study, we investigated the prevalence of sexually transmitted infections (stis) using multiplex real-time pcr assay in healthy korean women. we also evaluated the risk factors of stis, and compared the various factors between the sti-positive and the sti-negative groups. a total of 799 endocervical swab samples from healthy korean women who visited our hospital for general medical check-ups during january 2012 to october 2012 were included. eight stis including human papillomavirus (hpv) ... | 2014 | 24462432 |
| mycoplasma genitalium: clinical significance and diagnosis. | mycoplasma genitalium is considered the smallest self-replicating cell. it was first isolated in 1981, from 2 of 13 men with urethritis. mycoplasma genitalium causes urethritis, cervicitis and pelvic inflammatory disease. because of difficulties in cultivation, the diagnosis is based exclusively on pcr methodology. the recommended therapy for mycoplasma genitalium infections is azithromycin or doxycycline. development of macrolide resistance was shown to correlate with treatment failure. | 2013 | 24476610 |
| prevalence of genital mycoplasmas in asymptomatic male partners of women diagnosed as having chlamydial infections. | we examined 209 asymptomatic male partners of women diagnosed as having chlamydial infections for the prevalence of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum, and ureaplasma parvum in their first-voided urine (fvu) by nucleic acid amplification tests. quantification of leukocytes in fvu was performed by automated urine particle analyzers. two (1.0%) men were positive for n. gonorrhoeae, and 92 (44.0%) were positive for c. trac ... | 2014 | 24486047 |
| a cross-sectional study of mycoplasma genitalium infection and correlates in women undergoing population-based screening or clinic-based testing for chlamydia infection in london. | to determine mycoplasma genitalium infection and correlates among young women undergoing population-based screening or clinic-based testing for chlamydia infection. | 2014 | 24503298 |
| prediction of the persistence of mycoplasma genitalium after antimicrobial chemotherapy by quantification of leukocytes in first-void urine from patients with non-gonococcal urethritis. | mycoplasma genitalium is regarded as another pathogen of male non-gonococcal urethritis (ngu). failure to eradicate this mycoplasma is associated with persistent or recurrent ngu, but this mycoplasma is not routinely examined in clinical practice. in cases of m. genitalium-positive ngu, therefore, some criteria are needed to assess the success or failure of antimicrobial chemotherapy other than microbiological outcomes. we enrolled 49 men with m. genitalium-positive non-chlamydial ngu. at succes ... | 2014 | 24508423 |
| mycoplasma genitalium: a review. | mycoplasma genitalium (m. genitalium)was first isolated from the urethral swabs of two symptomatic men with urethritis in 1980. published prevalence rates vary greatly between populations studied. a number of urogenital conditions have been ascribed tom. genitalium, which is recognised to cause a sexually transmitted infection. the association of m. genitalium with non-specific urethritis is now well established, but the evidence supporting its role in both male and female infertility remains in ... | 2014 | 24517928 |
| cervical cytopathological findings in korean women with chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum infections. | this is to investigate the cervical cytological abnormalities associated with chlamydia trachomatis, mycoplasma hominis, mycoplasma genitalium, and ureaplasma urealyticum infections on routine screen. a total of 714 subjects who had undergone cervical pap smears and concomitant analyses for cervical infections were included by a retrospective search. the frequencies of reactive cellular change (rcc) and squamous epithelial abnormalities were significantly higher in chlamydia positive subjects th ... | 2014 | 24526918 |
| characterization of the operon encoding the holliday junction helicase ruvab from mycoplasma genitalium and its role in mgpb and mgpc gene variation. | mycoplasma genitalium is an emerging sexually transmitted pathogen associated with reproductive tract disease in men and women, and it can persist for months to years despite the development of a robust antibody response. mechanisms that may contribute to persistence in vivo include phase and antigenic variation of the mgpb and mgpc adhesins. these processes occur by segmental recombination between discrete variable regions within mgpb and mgpc and multiple archived donor sequences termed mgpa r ... | 2014 | 24532771 |
| distribution and perinatal transmission of bacterial vaginal infections in pregnant women without vaginal symptoms. | we evaluated the distribution and vertical transmission of bacterial vaginal infections in asymptomatic pregnant women. | 2014 | 24552584 |
| the future of whole-cell modeling. | integrated whole-cell modeling is poised to make a dramatic impact on molecular and systems biology, bioengineering, and medicine--once certain obstacles are overcome. from our group's experience building a whole-cell model of mycoplasma genitalium, we identified several significant challenges to building models of more complex cells. here we review and discuss these challenges in seven areas: first, experimental interrogation; second, data curation; third, model building and integration; fourth ... | 2014 | 24556244 |
| the prevalence of urethral and rectal mycoplasma genitalium among men who have sex with men in china, a cross-sectional study. | although mycoplasma genitalium (mg) is a common sexually transmitted infection (sti), very little information regarding the prevalence of mg among msm (men who have sex with men) is available in china. the objective of this study was to determine the prevalence of mg among msm in the city of shenzhen, guangdong province, china, and to identify the potential risk factors associated with mg infection in this population. | 2014 | 24559387 |
| direct detection of macrolide resistance in mycoplasma genitalium isolates from clinical specimens from france by use of real-time pcr and melting curve analysis. | mycoplasma genitalium is a sexually transmitted organism commonly treated with azithromycin. however, macrolide resistance has been reported and is associated with point mutations in the 23s rrna gene. to evaluate the prevalence of macrolide resistance in m. genitalium isolates from clinical specimens from france, we first used a previously reported high-resolution melting assay. because susceptible and resistant m. genitalium isolates were hardly discriminated in m. genitalium-positive clinical ... | 2014 | 24574291 |
| performance and comparison of self-reported sti symptoms among high-risk populations - msm, sex workers, persons living with hiv/aids - in el salvador. | resource-limited countries have limited laboratory capability and rely on syndromic management to diagnose sexually transmitted infections (stis). we aimed to estimate the sensitivity, specificity and positive predictive value (ppv) of sti syndromic management when used as a screening method within a study setting. men who have sex with men (msm), female sex workers (fsws) and people living with hiv/aids (plwha) participated in a behavioural surveillance study. data were obtained on demographics ... | 2014 | 24616119 |
| in vitro activity of the new fluoroketolide solithromycin (cem-101) against macrolide-resistant and -susceptible mycoplasma genitalium strains. | mycoplasma genitalium has become well established as an etiological agent of sexually transmitted infections, but due to its fastidious growth requirements, only a few m. genitalium strains are available to determine the mics of currently used and new antimicrobial agents. recent clinical trials have suggested that treatment with azithromycin has decreasing efficacy due to an increasing prevalence of macrolide resistance, and alternative treatment with moxifloxacin is similarly under pressure fr ... | 2014 | 24637681 |
| mycoplasma genitalium promotes epithelial crossing and peripheral blood mononuclear cell infection by hiv-1. | mycoplasma genitalium co-infection in hiv-infected individuals has been reported to increase the shedding of hiv in the urogenital region of females. to better understand this relationship, we investigated the influence of m. genitalium on the transmission and replication of hiv using an in vitro model. | 2014 | 24661929 |
| epidemiological and clinical rationale for screening and diagnosis of mycoplasma genitalium infections. | 2014 | 24669447 | |
| association between mycoplasma genitalium infection and hiv acquisition among female sex workers in uganda: evidence from a nested case-control study. | cross-sectional studies have shown a strong association between mycoplasma genitalium and hiv infections. we previously reported that in a cohort of female sex workers in uganda, m genitalium infection at baseline was associated with hiv seroconversion. here we examine the temporal association between the m genitalium infection status shortly before hiv seroconversion and hiv acquisition. | 2014 | 24687129 |
| uncoupling of the apyrimidinic/apurinic endonucleolytic and 3'→5' exonucleolytic activities of the nfo protein of mycoplasma pneumoniae through mutation of specific amino acid residues. | the dna recombination and repair machineries of mycoplasma pneumoniae and mycoplasma genitalium were predicted to consist of a set of ~11 proteins. the function of one of these proteins was inferred from its homology with proteins belonging to the endo iv enzyme family. the members of this family function in the repair of apyrimidinic/apurinic (ap) sites in dna. as such activity may be crucial in the mycoplasmal life cycle, we set out to study the endo iv-like proteins encoded by m. pneumoniae a ... | 2014 | 24694374 |
| mycoplasma genitalium testing pattern and macrolide resistance: a danish nationwide retrospective survey. | mycoplasma genitalium is a common cause of nongonococcal urethritis (ngu) and cervicitis. the aim of the study was to analyze the m. genitalium testing pattern and distribution of positive results according to sex and age in a 5-year period where all diagnostic m. genitalium testing in denmark was centralized at the statens serum institut. a secondary aim was to estimate the occurrence of macrolide resistance in a 3-year period. | 2014 | 24729494 |
| diagnostic and resistance testing for mycoplasma genitalium: what will it take? | 2014 | 24729505 | |
| mycoplasma genitalium infection is associated with microscopic signs of cervical inflammation in liquid cytology specimens. | cervicitis is a common clinical finding often attributed to sexually transmitted infections (stis), but no etiologic agent is identified in the majority of cases. in this study, we comparatively assessed inflammation among the common infectious etiologies of cervicitis and assessed the potential value of liquid cytology specimens for predicting stis. among 473 louisiana women at low risk for acquiring stis, the prevalences of mycoplasma genitalium, chlamydia trachomatis, neisseria gonorrhoeae, a ... | 2014 | 24759719 |
| diagnosis and antimicrobial treatment of mycoplasma genitalium infection: sobering thoughts. | the discovery of mycoplasma genitalium in 1980-1981 eventually led to it becoming recognized as an important cause of non-gonococcal urethritis in men and also some genital tract diseases in women. subsequent to the original isolation, further attempts failed over the next decade and reliable detection only became possible with the use of nucleic acid amplification techniques. although tetracyclines, particularly doxycycline, were the first choice for treatment of non-gonococcal urethritis prior ... | 2014 | 24834454 |
| remarkable increase in fluoroquinolone-resistant mycoplasma genitalium in japan. | we determined the prevalence of macrolide and fluoroquinolone resistance-associated mutations in mycoplasma genitalium dna specimens from men with non-gonococcal urethritis (ngu) and analysed their effects on antibiotic treatments of m. genitalium infections. | 2014 | 24894419 |
| characteristics of acute nongonococcal urethritis in men differ by sexual preference. | nongonococcal urethritis (ngu) is a common clinical syndrome, but no etiological agent is identified in a significant proportion of cases. whether the spectrum of pathogens differs between heterosexual men (msw) and men who have sex with men (msm) is largely unstudied but of considerable clinical relevance. a retrospective review was done using the electronic medical record database of melbourne sexual health centre, australia. cases were first presentations of symptomatic acute ngu with ≥ 5 pol ... | 2014 | 24899041 |
| sensitive simultaneous detection of seven sexually transmitted agents in semen by multiplex-pcr and of hpv by single pcr. | sexually transmitted diseases (stds) may impair sperm parameters and functions thereby promoting male infertility. to date limited molecular studies were conducted to evaluate the frequency and type of such infections in semen thus, we aimed at conceiving and validating a multiplex pcr (m-pcr) assay for the simultaneous detection of the following std pathogens in semen: chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, trichomonas vaginalis, herpes virus simplex (hsv) -1 and - ... | 2014 | 24921247 |
| prevalence of trichomonas vaginalis, mycoplasma genitalium and ureaplasma urealyticum in men with urethritis attending an urban sexual health clinic. | we conducted a study to determine the prevalence of trichomonas vaginalis (tv), mycoplasma genitalium (mg) and ureaplasma urealyticum (uu) in men with urethritis, attending an urban sexual health clinic, in order to inform screening and treatment policies. men attending an urban sexual health clinic between june 2011 and january 2012 were evaluated. urine samples were collected from men with urethritis and tested for chlamydia trachomatis (ct), neisseria gonorrhoeae (gc) and tv using transcripti ... | 2015 | 24925897 |
| supersize me: cronobacter sakazakii phage gap32. | cronobacter sakazakii is a gram-negative pathogen found in milk-based formulae that causes infant meningitis. bacteriophages have been proposed to control bacterial pathogens; however, comprehensive knowledge about a phage is required to ensure its safety before clinical application. we have characterized c. sakazakii phage vb_csam_gap32 (gap32), which possesses the second largest sequenced phage genome (358,663bp). a total of 571 genes including 545 protein coding sequences and 26 trnas were id ... | 2014 | 25010279 |
| molecular detection of chlamydia trachomatis and other sexually transmitted bacteria in semen of male partners of infertile couples in tunisia: the effect on semen parameters and spermatozoa apoptosis markers. | this study was undertaken to determine the prevalence of chlamydia trachomatis, mycoplasmas, and ureaplasmas in semen samples of the male partners of infertile couples and to investigate whether chlamydia trachomatis could initiate apoptosis in human spermatozoa. a total of 85 males partners of infertile couples undergoing routine semen analysis according to world health organization guidelines were included. specimens were examined for the presence of chlamydia trachomatis, neisseria gonorrhoea ... | 2014 | 25019616 |
| prevalence and determinants of sexually transmitted infections in women at risk undergoing abortion in a swiss primary care setting. | specific prevalence data are needed to assess the burden of sexually transmitted infections (stis) in specific settings where data are scant, such as women seeking early termination of pregnancy (top). we aimed to investigate the prevalence of stis and its determinants in women at risk (e. g. pathological cervical swab) undergoing top in a primary care setting in switzerland. | 2014 | 25051930 |
| sexually transmitted infections in women. | sexually transmitted infections (stis) are highly prevalent and cause a wide spectrum of disease. however, the majority of these infections may be unrecognized due to lack of overt signs or symptoms of infection. asymptomatic infections remain significant as a result of the potential for long-term sequelae, predominately in women, and the risks of complications during pregnancy as well as mother-to-child transmission. laboratory diagnostics play an important role in identifying infection and in ... | 2014 | 25083897 |
| positivity at test of cure following first-line treatment for genital mycoplasma genitalium: follow-up of a clinical cohort. | to describe antibiotic use for treatment of mycoplasma genitalium (mg) at an urban sexual health centre in australia. to describe mg positivity rates in those returning for 1 month test of cure (toc) following first-line antibiotic treatment for mg. | 2015 | 25096921 |
| clinical characteristics associated with mycoplasma genitalium among female sex workers in nairobi, kenya. | the prevalence of mycoplasma genitalium is high in vulnerable populations of women in low-resource settings. however, the epidemiology of infection in these populations is not well established. to determine the prevalence of mycoplasma genitalium and its association with cervical cytology and other correlates, we recruited 350 female sex workers (fsw) who were 18 to 50 years old in nairobi, kenya, for a cross-sectional study. a questionnaire was administered at baseline to obtain information on ... | 2014 | 25100823 |
| detection of infectious organisms in archival prostate cancer tissues. | seroepidemiological studies have reported associations between exposure to sexually transmitted organisms and prostate cancer risk. this study sought dna evidence of candidate organisms in archival prostate cancer tissues with the aim of assessing if a subset of these cancers show any association with common genital infections. | 2014 | 25106851 |
| mg428 is a novel positive regulator of recombination that triggers mgpb and mgpc gene variation in mycoplasma genitalium. | the human pathogen mycoplasma genitalium employs homologous recombination to generate antigenic diversity in the immunodominant mgpb and mgpc proteins. only recently, some of the molecular factors involved in this process have been characterized, but nothing is known about its regulation. here, we show that m. genitalium expresses n-terminally truncated reca isoforms via alternative translation initiation, but only the full-length protein is essential for gene variation. we also demonstrate that ... | 2014 | 25138908 |
| usefulness of quantifying leukocytes in first-voided urine to predict positivity for chlamydia trachomatis in asymptomatic men at high risk for chlamydial infection. | chlamydia trachomatis causes acute non-gonococcal urethritis, but some infected men are asymptomatic. we examined leukocytes in uncentrifuged first-voided urine (fvu) from asymptomatic men at high risk for chlamydial infection by automated urine particle analyzers to assess whether the quantification of urinary leukocytes could predict chlamydial infection in these men. we enrolled 209 asymptomatic men, whose female sexual partners had been diagnosed as having a genital chlamydial infection. the ... | 2014 | 25156010 |
| unusually low prevalence of mycoplasma genitalium in urine samples from infertile men and healthy controls: a prevalence study. | to detect mycoplasma genitalium in urine samples of infertile men and men without any signs of infection in order to investigate whether m. genitalium and other genital mycoplasmas (mycoplasma hominis and ureaplasma spp) are found more often in urine samples of infertile men than in asymptomatic controls and to determine resistance to macrolides. | 2014 | 25157184 |
| epidemiology of sexually transmitted infections in rural southwestern haiti: the grand'anse women's health study. | the study attempts to define socioeconomic, clinical, and laboratory correlates in vaginitis and other sexually transmitted infections in rural southwestern haiti. a convenience sample of subjects recruited from a rural women's health clinic and attending an established clinic at the haitian health foundation (hhf) clinic was studied. a standardized history and physical examination, including speculum examination, and collection of blood, urine, and vaginal swabs were obtained from the women at ... | 2014 | 25200263 |
| sexual partnership characteristics of african american women who have sex with women; impact on sexually transmitted infection risk. | african american women who have sex with women (wsw) are emerging as a population at risk for sexually transmitted infections (stis). the objectives of this study were to explore partnership characteristics for a cohort of african american wsw and evaluate those characteristics as potential risk factors for stis. in addition, we aimed to determine sti diagnoses and identify predictors of sti infection. | 2014 | 25211257 |
| does cervical ureaplasma/mycoplasma colonization increase the lower uterine segment bleeding risk during cesarean section among patients with placenta previa? a cross-sectional study. | the underlying inflammation of endometrium may impede normal implantation of placenta during pregnancy. our objective is to show cervical colonization of ureaplasma and/or mycoplasma as a marker of endometritis in pregnancies complicated with placenta previa that can be a risk factor for placenta accreta and peripartum hemorrhage. | 2014 | 25219820 |
| epidemiology of sexually transmitted infections in rural haitian men. | the study attempts to determine the prevalence of organisms associated with urethritis in men in rural southwestern haiti and to determine the association with demographic, clinical and laboratory variables. a standardised verbal interview was conducted; genital examinations were done; urethral swabs were collected for nucleic acid amplification testing, and first void urine was obtained for urinalysis. the mean participant age was 54; 88.8% lived in a rural area. swabs were positive for trichom ... | 2015 | 25228665 |
| sitafloxacin: antimicrobial activity against ciprofloxacin-selected laboratory mutants of mycoplasma genitalium and inhibitory activity against its dna gyrase and topoisomerase iv. | a sitafloxacin regimen is highly effective on mycoplasma genitalium infections, including those caused by the mycoplasmas harboring mutant topoisomerase iv with a quinolone resistance-associated amino acid change in parc. in this study, we evaluated sitafloxacin antimicrobial activities against m. genitalium, including the mycoplasmas with decreased susceptibilities to quinolones, by determining minimum inhibitory concentrations (mics) for the strain atcc 33530 and its 3 ciprofloxacin-selected m ... | 2015 | 25245990 |
| screening for chlamydia trachomatis and mycoplasma genitalium; is first void urine or genital swab best? | 2015 | 25253762 | |
| prevalence of chlamydia trachomatis, neisseria gonorrhoeae and mycoplasma genitalium in asymptomatic patients under 30 years of age screened in a french sexually transmitted infections clinic. | an increasing prevalence of sexually transmitted infections (sti) has been noted in france over the past decade. asymptomatic carriage may be high in patients infected with chlamydia trachomatis attending free and anonymous screening centres (cdag) and information, diagnosis and screening centres for sti (ciddist). in these centres, systematic c. trachomatis detection is recommended in women ≤25 years and in men ≤30 years. | 2014 | 25322708 |
| prevalence of trichomonas vaginalis by pcr in men attending a primary care urology clinic in south korea. | trichomonas vaginalis, a causative agent of trichomoniasis, may trigger symptomatic or asymptomatic nongonococcal urethritis and chronic prostatitis in men. despite the availability of highly sensitive diagnostic tests, such as nucleic acid amplification tests, including pcr, few prospective studies present data on male t. vaginalis infection in south korea. in the present study, the prevalence of t. vaginalis and associated clinical conditions were evaluated in 201 male patients from a primary ... | 2014 | 25352707 |
| isolation of mycoplasma genitalium from patients with urogenital infections: first report from the latin-american region. | isolation of mycoplasma genitalium from clinical specimens remains difficult. we describe a modified culture system based on vero cells grown in medium 199 with 2% foetal bovine serum (fbs). the culture system was evaluated using early passage m. genitalium strains m6271 and m6311 with growth monitoring by quantitative taqman pcr. eleven endocervical swabs and one male urethral swab positive by 16s rrna and mgpa1-3 pcrs were quantified and inoculated into vero cell suspensions in medium 199 supp ... | 2013 | 25356322 |
| development and utilization of a custom pcr array workflow: analysis of gene expression in mycoplasma genitalium and guinea pig (cavia porcellus). | transcriptome analysis is a powerful tool for evaluating molecular pathways central to maturation of specific biological processes and disease states. recently, pcr-based arrays have supplemented microarray and rna-seq methodologies for studying changes in gene expression levels. pcr arrays are a more cost efficient alternative, however commercially available assemblies are generally limited to only a few more widely researched species (e.g., rat, human, and mouse). consequently, the investigati ... | 2015 | 25358686 |
| mycoplasma genitalium and its resistance to azithromycin in incarcerated men from far north queensland. | this study examined the prevalence of mycoplasma genitalium in incarcerated men from far north queensland as well as the prevalence of macrolide resistance in identified isolates. overall, eight out of 140 [5.71% (95% ci 1.82-9.60)] urine samples tested positive and two out of eight (25%) samples carried a mutation in the 23s rrna gene associated with macrolide resistance. | 2014 | 25402451 |
| mycoplasma genitalium is associated with increased genital hiv type 1 rna in zimbabwean women. | mycoplasma genitalium is a common sexually transmitted infection associated with human immunodeficiency virus (hiv) infection. some studies suggest that m. genitalium may increase the risk of hiv acquisition. however, results have been inconsistent, and this association has never been examined longitudinally. | 2015 | 25404521 |
| [non-gonococcal infectious urethritis : pathogen spectrum and management]. | for many years an increase in cases of urethritis has been observed in western europe. in order to be able to combat this continuous rise, the perception of sexually transmitted diseases must be promoted, the clarification and screening must be intensified and therapy must be rapidly and correctly carried out. in addition to the commonest pathogens causing urethritis, namely chlamydia and gonococci, many other pathogenic microbes must be taken into consideration in the diagnostics. with respect ... | 2015 | 25410827 |
| isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female. | mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. the infections influenced form genital mycoplasmas specifically mycoplasma hominis and mycoplasma genitalium potentially affect reproductive disorders, and infertility. | 2014 | 25469132 |
| a major determinant for gliding motility in mycoplasma genitalium: the interaction between the terminal organelle proteins mg200 and mg491. | several mycoplasmas, such as the emergent human pathogen mycoplasma genitalium, developed a complex polar structure, known as the terminal organelle (to), responsible for a new type of cellular motility, which is involved in a variety of cell functions: cell division, adherence to host cells, and virulence. the to cytoskeleton is organized as a multisubunit dynamic motor, including three main ultrastructures: the terminal button, the electrodense core, and the wheel complex. here, we describe th ... | 2015 | 25471372 |
| [mycoplasma genitalium lipid-associated membrane proteins induce endocervical epithelial cells to express β-defensin-2]. | to observe the expression of human β-defensin-2 (hbd-2) induced by mycoplasma genitalium-derived lipid-associated membrane proteins (lamps) and its potential mechanism. | 2014 | 25481183 |
| diagnosis of common bacterial causes of urethritis in men by gram stain, culture and multiplex pcr. | urethritis is one of the most important causes of morbidity and mortality in developing countries. the aim of this study was to detect common bacterial causes of urethritis in men by gram stain, culture and multiplex pcr.185 male patients who presented at the skin and venereal clinic of the dhaka medical college, bangladesh with clinical symptoms suggestive of urethritis were enrolled in this study. urethral discharges were tested for detection of neisseria gonorrhoeae by gram stain, culture and ... | 2014 | 25500516 |
| performance evaluation of a dna chip assay in the identification of major genitourinary pathogens. | to prevent the recurrence of genitourinary infections and to reduce the risks of their complications, accurate and rapid diagnosis are required. stdetect® chip is a dna chip which allows for the simultaneous detection of 13 major genitourinary pathogens in a single vaginal swab or urine specimen. we evaluated the analytical performance of the stdetect® chip for detecting target pathogens that commonly cause genitourinary infections. | 2015 | 25533217 |
| macrolide resistance and azithromycin failure in a mycoplasma genitalium-infected cohort and response of azithromycin failures to alternative antibiotic regimens. | our aim was to determine the efficacy of 1 g azithromycin and alternative antibiotic regimens in a prospective cohort of mycoplasma genitalium-infected participants, and factors associated with azithromycin failure. | 2015 | 25537875 |
| etiology and antimicrobial susceptibility of pathogens responsible for urethral discharge among men in harare, zimbabwe. | periodic etiological surveillance of sexually transmitted infection (sti) syndromes is required to validate treatment algorithms used to control stis. however, such surveys have not been performed in zimbabwe over the past decade. | 2014 | 25581806 |
| performance evaluation of automated urine microscopy as a rapid, non-invasive approach for the diagnosis of non-gonococcal urethritis. | gram-stained urethral smear (gsus), the standard point-of-care test for non-gonococcal urethritis (ngu) is operator dependent and poorly specific. the performance of rapid automated urine flow cytometry (aufc) of first void urine (fvu) white cell counts (uwcc) for predicting mycoplasma genitalium and chlamydia trachomatis urethral infections was assessed and its application to asymptomatic infection was evaluated. | 2015 | 25614466 |
| identification of macrolide-resistant mycoplasma genitalium using real-time pcr. | mycoplasma genitalium is a common cause of non-gonococcal urethritis (ngu) in western europe, but is not routinely tested for in all clinics. a high prevalence of macrolide-resistant m. genitalium has been reported. an easy to use test that can predict likely macrolide treatment failure is potentially very valuable. we report the development of a rapid and reliable real-time pcr-assay which detects all relevant resistance loci in the m. genitalium 23s rrna gene. | 2015 | 25622510 |
| spontaneous regression of untreatable mycoplasma genitalium urethritis. | 2015 | 25634824 | |
| treatment efficacy, treatment failures and selection of macrolide resistance in patients with high load of mycoplasma genitalium during treatment of male urethritis with josamycin. | azithromycin has been widely used for mycoplasma genitalium treatment internationally. however, the eradication efficacy has substantially declined recent decade. in russia, josamycin (another macrolide) is the recommended first-line treatment for m. genitalium infections, however, no data regarding treatment efficacy with josamycin and resistance in m. genitalium infections have been internationally published. we examined the m. genitalium prevalence in males attending an sti clinic in moscow, ... | 2015 | 25645440 |
| [mycoplasma genitalium-derived lipid-associated membrane proteins negatively regulate cytokine secretion by inducing ho-1 expression in placental trophoblast cells]. | to observe the expression of heme oxygenase-1 (ho-1) in regulation of cytokines response induced by mycoplasma genitalium-derived lipid-associated membrane proteins (lamps) in placental trophoblast cells. | 2015 | 25652861 |
| trichomonas vaginalis infection: how significant is it in men presenting with recurrent or persistent symptoms of urethritis? | persistent or recurrent non-gonococcal urethritis has been reported to affect up to 10-20% of men attending sexual health clinics. an audit was undertaken to review the management of persistent or recurrent non-gonococcal urethritis in men presenting at whittall street clinic, birmingham, uk. detection of trichomonas vaginalis infection was with the newly-introduced nucleic acid amplification test. a total of 43 (8%) of 533 men treated for urethritis re-attended within three months with persiste ... | 2016 | 25663246 |
| prevalence and macrolide resistance of mycoplasma genitalium in south african women. | remnant specimens from 601 women obtained in a cross-sectional study from rural south africa were tested for mycoplasma genitalium. overall, 10.8% of women were infected with m. genitalium either in the vagina or in the rectum. macrolide resistance, although of low prevalence, in m. genitalium is described for the first time in sub-saharan africa. | 2015 | 25668646 |
| vertical transmission of bacterial eye infections, angola, 2011-2012. | to determine transmission rates for neonatal conjunctivitis causative microorganisms in angola, we analyzed 312 endocervical and 255 conjunctival samples from mothers and newborns, respectively, during 2011-2012. transmission rates were 50% for chlamydia trachomatis and neisseria gonorrhoeae and 10.5% for mycoplasma genitalium. possible pathogenic effects of m. genitalium in children's eyes are unknown. | 2015 | 25695394 |
| opportunities and pitfalls of molecular testing for detecting sexually transmitted pathogens. | in the last 20 years, nucleic acid amplification tests (naats) have gradually replaced traditional methods for the detection of sexually transmitted infections. naat technology comes with some considerable benefits for diagnosis, including increased sensitivity, rapid result turnaround and suitability for high throughput screening of asymptomatic individuals using more-readily available specimens. however, the transition to naat has not come without its problems. false-negative and false-positiv ... | 2015 | 25714587 |
| screening for chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma genitalium should it be integrated into routine pregnancy care in french young pregnant women? | chlamydia trachomatis, neisseria gonorrhoeae, and mycoplasma genitalium screening during pregnancy is not performed routinely in france. we conducted the first prospective study in 1004 women attending for routine antenatal care to determine the prevalence and risk factors for these bacterial infections. the overall prevalence of c. trachomatis, n. gonorrhoeae, and m. genitalium infections was 2.5%, 0%, and 0.8%, respectively. in patients aged 18-24 years, the prevalence increased to 7.9% for c. ... | 2015 | 25753079 |
| complete atomistic model of a bacterial cytoplasm for integrating physics, biochemistry, and systems biology. | a model for the cytoplasm of mycoplasma genitalium is presented that integrates data from a variety of sources into a physically and biochemically consistent model. based on gene annotations, core genes expected to be present in the cytoplasm were determined and a metabolic reaction network was reconstructed. the set of cytoplasmic genes and metabolites from the predicted reactions were assembled into a comprehensive atomistic model consisting of proteins with predicted structures, rna, protein/ ... | 2015 | 25765281 |
| first attempt to implement ophthalmia neonatorum prophylaxis in angola: microorganisms, efficacy, and obstacles. | purpose. to determine the efficacy of povidone-iodine (p-i) prophylaxis for ophthalmia neonatorum (on) in angola and to document maternal prevalence and mother-to-child transmission rates. methods. endocervical samples from mothers (n = 317) and newborn conjunctival smears (n = 245) were analysed by multiplex polymerase chain reaction (pcr) for chlamydia trachomatis (ct), neisseria gonorrhoeae (ng), and mycoplasma genitalium (mg). newborns were randomized into a noninterventional group and an in ... | 2015 | 25785190 |
| five-day azithromycin treatment regimen for mycoplasma genitalium infection also effectively eradicates chlamydia trachomatis. | 2015 | 25823977 | |
| in vitro antibacterial activity of azd0914 against human mycoplasmas and ureaplasmas. | in this study, susceptibilities were determined for azd0914, a spiropyrimidinetrione dna gyrase inhibitor, azithromycin, doxycycline, and levofloxacin against mycoplasma and ureaplasma species. the activity of azd0914 was comparable to that of levofloxacin and doxycycline against mycoplasma genitalium and mycoplasma pneumoniae. the azd0914 mic90 against mycoplasma hominis was 8-fold greater than that for levofloxacin. the azd0914 mic90 against ureaplasma species was 4-fold less than that for azi ... | 2015 | 25824220 |
| prevalence of mycoplasma genitalium and mycoplasma hominis in urogenital tract of brazilian women. | the role of mycoplasma hominis and m. genitalium in urogenital tract infections remains unknown. furthermore these mollicutes present a complex relationship with the host immune response. the role of inflammatory cytokines in infections also makes them good candidates to investigate bacterial vaginosis and mycoplasma genital infections. therefore, the aim of this study was to detect the above-mentioned mollicutes by quantitative polymerase chain reaction (qpcr) methodologies in vaginal swabs and ... | 2015 | 25886914 |
| development and clinical application of an invaderplus® assay for the detection of genital mycoplasmas. | we developed a pcr-based assay involving invader® technology for detection of the genital mycoplasmas of mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum, and ureaplasma parvum. we compared its performance with that of a pcr-microtiter plate hybridization assay, which we developed previously, in detecting genital mycoplasmas in first-voided urine (fvu) specimens from men with non-gonococcal urethritis. the tests targeting each of the genital mycoplasmas were specific for the res ... | 2015 | 25892209 |
| mycoplasma genitalium infection and female reproductive tract disease: a meta-analysis. | to determine the association between mycoplasma genitalium infection and female reproductive tract syndromes through meta-analysis, english-language, peer-reviewed studies were identified via pubmed, embase, biosis, cochrane library, and reference review. two reviewers independently extracted data. random-effects models were employed to calculate summary estimates, between-study heterogeneity was evaluated using i(2) statistics, publication bias was assessed via funnel plots and the begg and egg ... | 2015 | 25900174 |
| mycoplasma genitalium serum antibodies in infertile couples and fertile women. | the association between mycoplasma genitalium (m. genitalium) serum antibodies and infertility in women and men, as well as infertility subtypes, was investigated. | 2015 | 25921018 |
| a novel sigma factor reveals a unique regulon controlling cell-specific recombination in mycoplasma genitalium. | the mycoplasma genitalium mg428 protein shows homology to members of the sigma-70 family of sigma factors. herein, we found that mg428 activates transcription of reca, ruva and ruvb as well as several genes with unknown function. deletion of mg_428 or some of the up-regulated unknown genes led to severe recombination defects. single cell analyses revealed that activation of the mg428-regulon is a rare event under laboratory growth conditions. a conserved sequence with sigma-70 promoter architect ... | 2015 | 25925568 |
| detection of genital mycoplasmal infections among infertile females by multiplex pcr. | women reproductive system is a suitable environment for growth of various pathogen and nonpathogenic microorganisms. mycoplasmataceae is a family of bacteria which cause oligosymptomatic genital infections. the complications caused by these bacteria may lead to infertility in women. the aim of this study was detection of genital mycoplasma hominis, ureaplasmaurealyticum and mycoplasma genitalium among infertile females who referred to the infertility clinics. | 2014 | 25926957 |
| detection of mycoplasma genitalium from male primary urine specimens: an epidemiologic dichotomy with trichomonas vaginalis. | a total of 2750 male urines subjected to a transcription-mediated amplification (tma)-based mycoplasma genitalium assay yielded 188 positive results (6.84%). this rate was similar to chlamydia trachomatis (6.87%; p = 0.96) and greater than neisseria gonorrhoeae (4.0%) and trichomonas vaginalis (2.3%; p < 0.0002). mean age of m. genitalium-infected males (30.8) was similar to n. gonorrhoeae (p = 0.78) but less than t. vaginalis (mean, 41.6; p < 0.0001). a total of 266 sti clinic encounters had at ... | 2015 | 25934156 |
| internet-based recruitment system for hiv and sti screening for men who have sex with men in estonia, 2013: analysis of preliminary outcomes. | the aim of the current project was to develop an internet-based recruitment system for hiv and sexually transmitted infection (sti) screening for men who have sex with men (msm) in estonia in order to collect biological samples during behavioural studies. in 2013, an internet-based hiv risk-behaviour survey was conducted among msm living in estonia. after completing the questionnaire, all participants were offered anonymous and free-of-charge sti testing. they could either order a urine sample k ... | 2015 | 25953131 |
| the role of chlamydia trachomatis in high-risk human papillomavirus persistence among female sex workers in nairobi, kenya. | little is known about risk factors for persistent high-risk human papillomavirus (hrhpv) infection in low-income settings, and prior research has not quantified the relative duration of hrhpv infections stratified by risk factors. we compared the duration of hrhpv infection among female sex workers (fsws) by exposure to sexually transmitted infections (stis), using a highly sensitive biomarker assay. | 2015 | 25970306 |
| drug resistance-associated mutations in mycoplasma genitalium in female sex workers, japan. | mycoplasma genitalium was detected in 21 (14.1%) of 149 vaginal swab samples and in 1 (0.7%) of 149 throat washing samples from female sex workers during 2013-2014 in japan. prevalences of m. genitalium with macrolide resistance-associated 23s rrna mutations and fluoroquinolone resistance-associated parc alterations were 47.1% and 36.8%, respectively. | 2015 | 25988775 |
| high levels of macrolide resistance-associated mutations in mycoplasma genitalium warrant antibiotic susceptibility-guided treatment. | mycoplasma genitalium is a sexually transmitted pathogen, and infection with it is usually treated with macrolides. unfortunately, emerging resistance to the macrolides has been associated with mutations in region v of the 23s rrna gene. the aim of this retrospective study was to describe the incidence of macrolide resistance-associated mutations in m. genitalium from patients in the netherlands. | 2015 | 25995292 |
| 2015 uk national guideline on the management of non-gonococcal urethritis. | we present the updated british association for sexual health and hiv guideline for the management of non-gonococcal urethritis in men. this document includes a review of the current literature on its aetiology, diagnosis and management. in particular it highlights the emerging evidence that azithromycin 1 g may result in the development of antimicrobial resistance in mycoplasma genitalium and that neither azithromycin 1 g nor doxycycline 100 mg twice daily for seven days achieves a cure rate of ... | 2016 | 26002319 |
| prevalence of sexually transmitted pathogens associated with hpv infection in cervical samples in a mexican population. | cervical cancer development has been mainly associated with persistent human papillomavirus (hpv) infections. however, hpv infection is unlikely to be sufficient to cause cervical cancer, and the contribution of other sexually transmitted infections (stis) could be the determining factor for cervical lesion-progression. the aim of this study was to estimate the prevalence of stis associated with hpv-positivity in 201 cervical samples from patients who underwent annual routine gynecological exams ... | 2015 | 26010580 |
| sexually transmitted diseases treatment guidelines, 2015. | these guidelines for the treatment of persons who have or are at risk for sexually transmitted diseases (stds) were updated by cdc after consultation with a group of professionals knowledgeable in the field of stds who met in atlanta on april 30-may 2, 2013. the information in this report updates the sexually transmitted diseases treatment guidelines, 2010 (mmwr recomm rep 2010;59 [no. rr-12]). these updated guidelines discuss 1) alternative treatment regimens for neisseria gonorrhoeae; 2) the u ... | 2015 | 26042815 |
| mycoplasma genitalium infection: current treatment options, therapeutic failure, and resistance-associated mutations. | mycoplasma genitalium is an important cause of non-gonococcal urethritis, cervicitis, and related upper genital tract infections. the efficacy of doxycycline, used extensively to treat non-gonococcal urethritis in the past, is relatively poor for m. genitalium infection; azithromycin has been the preferred treatment for several years. research on the efficacy of azithromycin has primarily focused on the 1 g single-dose regimen, but some studies have also evaluated higher doses and longer courses ... | 2015 | 26060411 |
| loop-mediated isothermal amplification (lamp) for the rapid detection of mycoplasma genitalium. | mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. due to the difficulty of the culture of m. genitalium from clinical samples, the laboratory diagnosis of m. genitalium infection is almost exclusively carried out using nucleic acid amplification tests. loop-mediated isothermal amplification (lamp) is a novel nucleic acid amplification technology, utilising a set of 4 primers specific to 6 distinct re ... | 2015 | 26072150 |
| sensitive detection of thirteen bacterial vaginosis-associated agents using multiplex polymerase chain reaction. | bacterial vaginosis (bv) is characterized by a polymicrobial proliferation of anaerobic bacteria and depletion of lactobacilli, which are components of natural vaginal microbiota. currently, there are limited conventional methods for bv diagnosis, and these methods are time-consuming, expensive, and rarely allow for the detection of more than one agent simultaneously. therefore, we conceived and validated a multiplex polymerase chain reaction (m-pcr) assay for the simultaneous screening of thirt ... | 2015 | 26078959 |
| which sexually active young female students are most at risk of pelvic inflammatory disease? a prospective study. | to identify risk factors for pelvic inflammatory disease (pid) in female students. | 2015 | 26082320 |
| male circumcision and sti acquisition in britain: evidence from a national probability sample survey. | it is well-established that male circumcision reduces acquisition of hiv, herpes simplex virus 2, chancroid, and syphilis. however, the effect on the acquisition of non-ulcerative sexually transmitted infections (stis) remains unclear. we examined the relationship between circumcision and biological measures of three stis: human papillomavirus (hpv), chlamydia trachomatis and mycoplasma genitalium. | 2015 | 26083250 |
| a phase 2 trial of oral solithromycin 1200 mg or 1000 mg as single-dose oral therapy for uncomplicated gonorrhea. | progressive resistance to antimicrobial agents has reduced options for gonorrhea therapy worldwide. solithromycin (cem-101) is a novel oral fluoroketolide antimicrobial with substantial in vitro activity against neisseria gonorrhoeae. | 2015 | 26089222 |
| large two-centre study into the prevalence of mycoplasma genitalium and trichomonas vaginalis in the netherlands. | mycoplasma genitalium and trichomonas vaginalis are common sexually transmitted infections (stis). in the netherlands, testing for m. genitalium and t. vaginalis is not recommended for first-line sti screening. recent reports about the increasing antimicrobial resistance in m. genitalium raise concern about the adequacy of current empirical treatment regimens. it is necessary to have insight in the prevalence of m. genitalium and t. vaginalis in order to evaluate current first-line sti screening ... | 2016 | 26199286 |
| dna microarray characterization of pathogens associated with sexually transmitted diseases. | this study established a multiplex pcr-based microarray to detect simultaneously a diverse panel of 17 sexually transmitted diseases (stds)-associated pathogens including neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma, herpes simplex virus (hsv) types 1 and 2, and human papillomavirus (hpv) types 6, 11, 16, 18, 31, 33, 35, 39, 54 and 58. the target genes are 16s rrna gene for n. gonorrhoeae, m. genitalium, m. hominism, and ureaplasma, the majo ... | 2015 | 26208181 |
| management of non-gonococcal urethritis. | non-gonococcal urethritis (ngu), or inflammation of the urethra, is the most common treatable sexually transmitted syndrome in men, with approximately 20-50 % of cases being due to infection with chlamydia trachomatis and 10-30 % mycoplasma genitalium. other causes are ureaplasma urealyticum, trichomonas vaginalis, anaerobes, herpes simplex virus (hsv) and adenovirus. up to half of the cases are non-specific. urethritis is characterized by discharge, dysuria and/or urethral discomfort but may be ... | 2015 | 26220178 |