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clinicoimmunopathologic findings in atlantic bottlenose dolphins tursiops truncatus with positive chlamydiaceae antibody titers.sera from free-ranging atlantic bottlenose dolphins tursiops truncatus inhabiting the indian river lagoon (irl), florida, and coastal waters of charleston (chs), south carolina, usa, were tested for antibodies to chlamydiaceae as part of a multidisciplinary study of individual and population health. a suite of clinicoimmunopathologic variables was evaluated in chlamydiaceae-seropositive dolphins (n = 43) and seronegative healthy dolphins (n = 83). fibrinogen, lactate dehydrogenase, amylase, and ...201424492056
erysipelothrix rhusiopathiae contamination in the poultry house environment during erysipelas outbreaks in organic laying hen flocks.this study investigated organic laying hen farms for the presence of erysipelothrix rhusiopathiae in the house environment and from potential carriers (i.e. insects and mice) during ongoing erysipelas outbreaks, and compared the obtained isolates with those from laying hens. the samples were investigated by selective culture followed by species-specific polymerase chain reaction on cultures. e. rhusiopathiae was isolated from the spleen, jejunal contents, manure, dust and swabs from water nipple ...201424661145
erysipelothrix rhusiopathiae bacteremia without endocarditis associated with psoas abscess: the first case report in thailand.the authors report a patient with a rare manifestation of invasive septic erysipelothrix rhusiopathiae infection without endocardial involvement. our patient presented with progressive paraparesis and subacute fever for ten days. he had underlying diabetes mellitus and alcoholic cirrhosis. magnetic resonance imaging (mri) of the lumbosacral spine showed a psoas abscess with vertebral osteomyelitis and discitis at level of l23 of the lumbar spine. his blood culture grew e. rhusiopathiae. transtho ...201424772603
phosphorylcholine and spaa, a choline-binding protein, are involved in the adherence of erysipelothrix rhusiopathiae to porcine endothelial cells, but this adherence is not mediated by the paf receptor.a crucial event in the initiation of many bacterial infections is the adherence of the bacteria to host cells, and bacterial surface structures and their interactions with host cell receptors play an important role in this process. erysipelothrix rhusiopathiae is the causative agent of swine erysipelas, which may cause acute septicemia or chronic endocarditis and polyarthritis. to study the pathogenic mechanism of the widespread vascular disease observed in the acute form of swine erysipelas, we ...201424856134
erysipelas in a free-range layer flock with conjunctival oedema as an unusual clinical sign.erysipelas was diagnosed in a free-range laying flock with a high mortality of up to 7% per day and a severe decrease in egg production to 45%. the disease had a short course and unusual clinical features for erysipelas, including swollen, lacrimating and encrusted eyes. bacteriologically, trapped poultry red mites and affected animals were culture-positive for erysipelothrix rhusiopathiae. isolates from layers and mites were both serotype 1b. histopathology revealed disseminated intravasal coag ...201424881267
complete genome assembly and characterization of an outbreak strain of the causative agent of swine erysipelas--erysipelothrix rhusiopathiae sy1027.erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and, to a fewer occurrences, human erysipeloid. it is ubiquitous in nature and commensal in diverse species of animals, wild or domestic, from mammals and birds to reptiles and fish. mechanisms of its virulence and pathogenicity are poorly understood.201424993343
development of an indirect immunofluorescence technique for the evaluation of generated antibody titers against erysipelothrix rhusiopathiae in captive bottlenose dolphins (tursiops truncatus).erysipelothrix rhusiopathiae is the causative agent of erysipelas, a disease of many mammalian and avian species, mainly swine and turkeys. in cetaceans, erysipelas is considered to be the most common infection in juvenile individuals, which have not been vaccinated. moreover, the disease manifest in both forms, the dermatologic and the acute septicemic forms, has been reported in various species of dolphins and whales. it is difficult to diagnose erysipelas by currently available approaches. mo ...201425064337
emerging causes of superficial and invasive infections following marine injuries and exposures.soft tissue bacterial infections following aquatic animal bites, stings, and minor injuries occur commonly and usually on the extremities in fishermen and beachgoers worldwide after freshwater and saltwater exposures. louisiana has more tidal, saltwater, and brackish water shorelines (more than 7,000 miles) than any other state, including alaska and hawaii. as a result, louisiana residents are often exposed to marine pathogens when fishing or working offshore or when enjoying louisiana's miles o ...201725075726
first report of erysipelothrix rhusiopathiae-associated septicaemia and histologic changes in cultured australian eels, anguilla reinhardtii (steindachner, 1867) and a. australis (richardson, 1841). 201525080974
antibacterial activity of the essential oils of myrtle leaves against erysipelothrix rhusiopathiae.to evaluate the antibacterial activity of the essential oil of myrtus communis (m. communis) l. against erysipelothrix rhusiopathiae (e. rhusiopathiae) in vitro.201425183140
role of surface protective antigen a in the pathogenesis of erysipelothrix rhusiopathiae strain c43065.to clarify the role of surface protective antigen a (spaa) in the pathogenesis of erysipelothrix rhusiopathiae c43065 (serotype 2), the spaa deletion mutant of e. rhusiopathiae δspaa was constructed by homologous recombination. the virulence of the δspaa mutant decreased more than 76-fold compared with that of the wild-type strain c43065 in mice. the mutant strain was sensitive to the bactericidal action of swine serum, whereas the wild-type strain was resistant. the adhesion of wild-type strain ...201525223326
acute and chronic erysipelothrix rhusiopathiae infection in lambs.polyarthritis caused by erysipelothrix rhusiopathiae is a relatively common infection in lambs characterized by low mortality and high morbidity. e. rhusiopathiae is a ubiquitous gram-positive bacterium that is both a commensal and a pathogen of vertebrates. the disease was studied during an outbreak in a norwegian spæl sheep flock. in the acute phase, 48 of 230 (20%) lambs developed clinical signs and 4 died (1.7%). one acute case was necropsied and e. rhusiopathiae was cultured from all major ...201525377692
first case of osteomyelitis due to erysipelothrix rhusiopathiae: pubic osteomyelitis in a gored farmer.we report the first proven case of osteomyelitis due to erysipelothrix rhusiopathiae. this infection occurred almost 20 years after traumatic inoculation of the bacterium, when the patient was gored by one of his cows. diagnosis was made by bone biopsies, and treatment included rifampicin and levofloxacin for 3 months.201525462176
bacterial zoonoses of fishes: a review and appraisal of evidence for linkages between fish and human infections.human contact with and consumption of fishes presents hazards from a range of bacterial zoonotic infections. whereas many bacterial pathogens have been presented as fish-borne zoonoses on the basis of epidemiological and phenotypic evidence, genetic identity between fish and human isolates is not frequently examined or does not provide support for transmission between these hosts. in order to accurately assess the zoonotic risk from exposure to fishes in the context of aquaculture, wild fisherie ...201525466575
erysipelothrix rhusiopathiae and mycoplasma hyopneumoniae: the sensitivities of enzyme-linked immunosorbent assays for detecting vaccinated sows of unknown disease status using serum and colostrum, and the correlation of the results for sow serum, colostrum, and piglet serum.due to relatively high concentrations of immunoglobulins, colostrum has the potential to improve the sensitivity of diagnostic tests for diseases in pigs when compared with serum. it is possible that colostrum could improve the sensitivity of the antibody enzyme-linked immunosorbent assay (elisa) compared with serum. colostrum is also essential for piglets, providing protection against infections in the first few weeks and months of life. the sensitivity of 2 commercially available elisas, one f ...201525613041
characterization of erysipelothrix rhusiopathiae strains isolated from acute swine erysipelas outbreaks in eastern china.recently, a series of acute swine erysipelas outbreaks occurred in eastern china. eight strains isolated from cases of septicemia were determined as serotype 1a, and 4 of the isolates were resistant to acriflavine. one isolate strain named hx130709 was attenuated on agar media containing acriflavine dye. the 432-bp hypervariable region in spaa gene of the field and attenuated strains were amplified and sequenced. it was further compared with the vaccine strain g4t10, and thus, the eight field st ...201525649849
molecular detection of avian pathogens in poultry red mite (dermanyssus gallinae) collected in chicken farms.poultry red mite (prm, dermanyssus gallinae) is a blood-sucking ectoparasite as well as a possible vector of several avian pathogens. in this study, to define the role of prm in the prevalence of avian infectious agents, we used polymerase chain reaction (pcr) to check for the presence of seven pathogens: avipox virus (apv), fowl adenovirus (fadv), marek's disease virus (mdv), erysipelothrix rhusiopathiae (er), salmonella enterica (se), mycoplasma synoviae (ms) and mycoplasma gallisepticum (mg). ...201425649939
first report of macrolide resistance gene erm(t) harbored by a novel small plasmid from erysipelothrix rhusiopathiae.the macrolide resistance gene erm(t) was identified for the first time in a porcine erysipelothrix rhusiopathiae isolate from swine in china. the novel 3,749-bp small plasmid per29, which carries erm(t), had a g+c content of 31% and four distinct open reading frames. the presence of per29 increased by at least 128-fold the mics of clindamycin and erythromycin for e. rhusiopathiae. the fitness cost of per29 could be responsible for the low frequency of erm(t) in e. rhusiopathiae.201525666150
presence and new genetic environment of pleuromutilin-lincosamide-streptogramin a resistance gene lsa(e) in erysipelothrix rhusiopathiae of swine origin.erysipelothrix rhusiopathiae is a gram-positive bacillus that causes erysipelas in swine. in recent years, erysipelas infection among swine in china has been increasing. a combined resistance phenotype to pleuromutilins, lincosamides, and streptogramin a (plsa phenotype) was found in some e. rhusiopathiae isolates. the aim of this study was to identify the resistance genes responsible for the plsa phenotype in e. rhusiopathiae strains and to map the genetic environment of the identified resistan ...201525759293
exposure of extensively farmed wild boars (sus scrofa scrofa) to selected pig pathogens in greece.increased density and distribution of wild boar populations are likely to promote interactions and transmission of certain pathogens, not only among wild boar but also from wild boar to livestock or humans and vice versa.201525774434
erysipelothrix rhusiopathiae-induced aortic valve endocarditis: case report and literature review.erysipelothrix rhusiopathiae is a pathogen of zoonosis often associated with occupational exposure. although erysipelothrix rhusiopathiae infection has high mortality, the heart valves in humans are rarely involved. the clinical data of a case of a 65-year-old male with erysipelothrix rhusiopathiae-induced aortic valve endocarditis was summarized retrospectively and analyzed with a literature review. based on a literature review and our experience, cases of e. rhusiopathiae-induced aortic valve ...201525785050
sepsis due to erysipelothrix rhusiopathiae in a patient with chronic lymphocytic leukemia associated with bronchopneumonia due to pseudomonas aeruginosa and escherichia coli: a case report.the present report describes a case of sepsis due to erysipelothrix rhusiopathiae in a patient with b-cell chronic lymphocytic leukemia with no animal exposure, associated with concomitant bronchopneumonia due to pseudomonas aeruginosa and escherichia coli.201726015797
erysipelothrix rhusiopathiae associated with recent widespread muskox mortalities in the canadian arctic. 201526028673
safety and efficacy of a new octavalent combined erysipelas, parvo and leptospira vaccine in gilts against leptospira interrogans serovar pomona associated disease and foetal death.the safety and protective efficacy of a new octavalent combination vaccine containing inactivated erysipelothrix rhusiopathiae, parvovirus, and leptospira interrogans (sensu lato) serogroups canicola, icterohaemorrhagiae, australis (bratislava), grippotyphosa, pomona and tarassovi - porcilis(®) ery+parvo+lepto - was evaluated in laboratory studies and under field conditions. the safety (2× overdose and repeated dose) was tested in 26 gilts. in this study, neither vaccine related temperature incr ...201526100922
development of an snp-based pcr assay for rapid differentiation of a japanese live vaccine strain from field isolates of erysipelothrix rhusiopathiae.the differentiation of vaccine from non-vaccine isolates is important for disease control. based on single nucleotide polymorphisms identified by comparison of the genomes of the koganei 65-0.15 vaccine strain and a reference strain of erysipelothrix rhusiopathiae, we developed a pcr assay that can differentiate the vaccine strain from field isolates.201526151408
a combinational approach of multilocus sequence typing and other molecular typing methods in unravelling the epidemiology of erysipelothrix rhusiopathiae strains from poultry and mammals.erysipelothrix rhusiopathiae infections re-emerged as a matter of great concern particularly in the poultry industry. in contrast to porcine isolates, molecular epidemiological traits of avian e. rhusiopathiae isolates are less well known. thus, we aimed to (i) develop a multilocus sequence typing (mlst) scheme for e. rhusiopathiae, (ii) study the congruence of strain grouping based on pulsed-field gel electrophoresis (pfge) and mlst, (iii) determine the diversity of the dominant immunogenic pro ...201526198736
fatal erysipelothrix rhusiopathiae septicemia in two atlantic dolphins (stenella frontalis and tursiops truncatus).we describe gross, histopathologic, ultrastructural, immunohistochemical, and microbiologic features of acute septicemia by erysipelothrix rhusiopathiae in an atlantic spotted dolphin stenella frontalis and an atlantic bottlenose dolphin tursiops truncatus. generalized lymphadenomegaly and widespread hemorrhages were the most consistent macroscopic findings. tricavitary effusion and icterus were noted in one individual. histologically, all organs examined showed numerous variably sized bacillary ...201526378410
osteochondrosis, but not lameness, is more frequent among free-range pigs than confined herd-mates.organic pig production is expanding and amongst the objectives of organic farming are enhancing animal health and welfare. however, some studies have reported a higher prevalence of lameness and joint condemnation at slaughter in free-range/organic pigs than in conventionally raised pigs. organic slaughter pigs have free-range housing in which indoor and outdoor access is compulsory, while in conventional farming the pigs are commonly confined to indoor pens. the present study evaluated the effe ...201526416598
erysipelas outbreaks in flocks of geese in poland--biochemical and genetic analyses of the isolates.erysipelothrix rhusiopathiae causes erysipelas in many vertebrate species. severe disease outbreaks have been noted in many poultry species--chickens, ducks, emus, pheasants, pigeons, and geese. this article describes the biochemical and genetic analyses of six e. rhusiopathiae strains isolated from geese for meat production. the isolates came from birds kept in different poultry houses on one farm, and were collected during two erysipelas outbreaks. we analyzed and compared the isolates by rand ...201526478164
tenosynovitis of a digit due to erysipelothrix rhusiopathiae: case report and review of the literature.erysipelothrix rhusiopathiae is a gram-positive bacterium that in humans causes skin infections, such as erysipeloid, as a result of direct contact with contaminated animals or their waste or products. we present the first reported case of tenosynovitis of a finger in a 30-year-old woman.201526693283
unusual substrate specificity of the peptidoglycan mure ligase from erysipelothrix rhusiopathiae.erysipelothrix rhusiopathiae is a gram-positive bacterium pathogenic to many species of birds and mammals, including humans. the main feature of its peptidoglycan is the presence of l-alanine at position 3 of the peptide stem. in the present work, we cloned the mure gene from e. rhusiopathiae and purified the corresponding protein as his6-tagged form. enzymatic assays showed that e. rhusiopathiae mure was indeed an l-alanine-adding enzyme. surprisingly, it was also able, although to a lesser ext ...201626700151
fluorescent microbead-based immunoassay for anti-erysipelothrix rhusiopathiae antibody detection in cetaceans.a fluorescent microbead-based immunoassay (fmia) for detection of anti-erysipelothrix rhusiopathiae antibodies in pigs was adapted for use in cetaceans. the fmia was validated and adjusted using serum samples from 10 vaccinated captive bottlenose dolphins tursiops truncatus collected between 1 and 13 mo after immunization. the technique was then used to analyze specimens from 15 free-ranging cetaceans stranded alive on the valencian mediterranean coast between 2006 and 2014: 11 striped dolphins ...201626758657
human erysipelothrix rhusiopathiae infection: unsolved issues and possible solutions.erysipelothrix rhusiopathiae infection in humans may not be as rare as previously thought. in most cases, the disease is acquired from animals through work-related exposure. human infection has been reported since the early 1900's up to the present. unsolved issues associated with this organism include inadequate disease control in animals, difficulty in identification and isolation of the bacteria, diagnostic delay due to unawareness of this uncommon disease or unfamiliarity with the increasing ...201526817228
virulence determinants, antimicrobial susceptibility, and molecular profiles of erysipelothrix rhusiopathiae strains isolated from china.the aim of this study was to understand the epidemiology, serotype, antibiotic sensitivity, and clonal structure of erysipelothrix rhusiopathiae strains in china. forty-eight strains were collected from seven provinces during the period from 2012 to 2013. pulse-field electrophoresis identified 32 different patterns which were classified into clonal groups a–d. most pulsed-field gel electrophoresis (pfge) patterns were observed in clonal complex a and b, suggesting high diversity of genetic chara ...201526975059
reproduction of post-weaning multi-systemic wasting syndrome in an animal disease model as a tool for vaccine testing under controlled conditions.snatch farrowed, colostrum deprived piglets were inoculated with different combinations of porcine circovirus 2, porcine parvovirus and erysipelothrix rhusiopathiae candidate vaccines. 10 piglets were mock-vaccinated. following virus challenge with a combined porcine circovirus 2/porcine parvovirus inoculum, all animals were monitored and samples taken for serology, immunohistochemistry and qpcr. at 24 dpc all non-vaccinated animals remaining were exhibiting signs of post-weaning multi-systemic ...201627033924
effects of amoxicillin, ceftiofur, doxycycline, tiamulin and tulathromycin on pig humoral immune responses induced by erysipelas vaccination.it addition to their antimicrobial properties, antibiotics can influence the host immune system (modulation of cytokine secretion, antibody production and t-cell proliferation). in the present study, the authors studied the effects of therapeutic doses of amoxicillin (amx), ceftiofur (cef), doxycycline (doxy), tiamulin (tiam) and tulathromycin (tul) on the postvaccinal immune response after pigs had been vaccinated against erysipelas. because humoral immunity is considered as the most important ...201627072375
erysipelothrix rhusiopathiae bacteremia without endocarditis: rapid identification from positive blood culture by maldi-tof mass spectrometry. a case report and literature review.erysipelothrix rhusiopathiae is a gram-positive bacillus that is infrequently responsible for infections in humans. three forms have been classified: a localized cutaneous form (erysipeloid) caused by traumatic penetration of e. rhusiopathiae, a generalized cutaneous form and a septicemic form. the latter type of disease has been previously associated with a high incidence of endocarditis. here we report a case of e. rhusiopathiae bacteremia in a 74-year-old man, probably started from an erysipe ...201627103974
systemic erysipelothrix rhusiopathiae infection not associated with endocarditis highlighting bacteriological diagnosis difficulties case report and literature review.erysipelothrix rhusiopathiae is mostly isolated in swine causing erysipelas. human invasive infections due to e. rhusiopathiae remain poorly described and interestingly bacteraemia associated with endocarditis are a source of ineffective empirical antibiotherapy. we report a case of sepsis without endocarditis due to e. rhusiopathiae and a review of the literature.201627112902
endocarditis associated with erysipelothrix rhusiopathiae in a fat-tailed ram.endocarditis is rarely reported in sheep and information presented for ovine endocarditis is based mostly on comparative findings in the cattle. infective vegetative endocarditis of the right heart was diagnosed in a 3-year-old fat-tailed ram. clinical findings included tachycardia, marked brisket edema, jugular veins distention and pulsation and pale mucous membranes. hematologic abnormality included neutrophilic leukocytosis. necropsy confirmed severe right atrioventricular and pulmonary valve ...201527175196
complete genome sequence of erysipelothrix rhusiopathiae strain gxby-1 isolated from acute swine erysipelas outbreaks in south china.erysipelothrix rhusiopathiae (e. rhusiopathiae) is an important pathogenic microorganism affecting swine industry. here, we report the finished annotated genome sequence of e. rhusiopathiae gxby-1, isolated from acute swine erysipelas in binyang county, guangxi, china. the gxby-1 strain, which exhibits high pathogenicity for swine, contains 1,876,490 bp with g + c content of 36.50%, and contains 1734 protein-coding genes, 57 trnas and 27 rrnas. the nucleotide sequence of this genome was deposite ...201627222802
genomic analysis of the multi-host pathogen erysipelothrix rhusiopathiae reveals extensive recombination as well as the existence of three generalist clades with wide geographic distribution.knowledge about how bacterial populations are structured is an important prerequisite for studying their ecology and evolutionary history and facilitates inquiry into host specificity, pathogenicity, geographic dispersal and molecular epidemiology. erysipelothrix rhusiopathiae is an opportunistic pathogen that is currently reemerging in both the swine and poultry industries globally. this bacterium sporadically causes mortalities in captive marine mammals, and has recently been implicated in lar ...201627301771
[identifying immunogenic proteins of erysipelothrix rhusiopathiae c43065 by maldi-tof mass spectrometry and cloning their encoding genes].to identify immunogenic proteins of erysipelothrix rhusiopathiae c43065.201627373076
proteomic and transcriptomic analyses of swine pathogen erysipelothrix rhusiopathiae reveal virulence repertoire.e. rhusiopathiae is the causative agent of erysipelas in animals and erysipeloid in humans, but its pathogenicity is poorly understood. to identify virulence factors associated with e. rhusiopathiae and screen engineered vaccine candidates, we used proteomics and transcriptomics to compare the highly virulent strain hx130709 with an isogenic avirulent derivative, hx130709a. 1,299 proteins and 1,673 transcribed genes were identified and 1,292 of the proteins could be associated with genes. in a c ...201627479071
genomic and proteomic characterization of se-i, a temperate bacteriophage infecting erysipelothrix rhusiopathiae.a bacteriophage infecting pathogenic erysipelothrix rhusiopathiae was isolated from a swine farm experiencing an outbreak of acute swine erysipelas; we designated this phage se-i. se-i has an icosahedral head, a long tail and a double-stranded dna genome. the 34,997-bp genome has a gc content of 34 % and contains 43 open reading frames (orfs) encoding packaging, structural, lysin-holin, and hypothetical proteins. components of purified se-i were separated using sds-page and analyzed using liquid ...201627541818
shaggy lame fox syndrome in pribilof island arctic foxes ( alopex lagopus pribilofensis), alaska.a previously unrecognized condition is described in wild free-ranging pribilof arctic foxes ( alopex lagopus pribilofensis) from the pribilof islands, alaska, usa. this condition is called shaggy lame fox syndrome (slfs) denoting the primary clinical signs first observed. criteria used to suspect slfs on gross examination included emaciation, failure to shed winter pelage and moderate to severe polyarthritis. criteria used to confirm slfs histologically included polyarthritis (characterized by l ...201727543449
erysipelothrix rhusiopathiae isolated from gull-inflicted wounds in southern right whale calves.southern right whales eubalaena australis from peninsula valdés, argentina, show wounds produced by kelp gulls larus dominicanus that feed on the whales' dorsal skin and blubber. during the 2013 whale season, several calves were reported showing kelp gull injuries with a swollen area surrounded by rhomboid-shaped raised edges. samples from 9 calves were taken in order to establish the etiology of these rhomboid-shaped wounds; 2 calves (one living, one dead) showed gull-inflicted injuries with rh ...201627596861
review of nonfoodborne zoonotic and potentially zoonotic poultry diseases.emerging and re-emerging diseases are continuously diagnosed in poultry species. a few of these diseases are known to cross the species barrier, thus posing a public health risk and an economic burden. we identified and synthesized global evidence for poultry nonfoodborne zoonoses to better understand these diseases in people who were exposed to different poultry-related characteristics (e.g., occupational or nonoccupational, operational types, poultry species, outbreak conditions, health status ...201627610715
evaluation and improvement of a single nucleotide polymorphism-based pcr assay for rapid differentiation of live attenuated vaccine strains from field isolates of erysipelothrix rhusiopathiae.a single nucleotide polymorphism-based pcr assay has been developed to differentiate the attenuated vaccine strain used in japan from field isolates of erysipelothrix rhusiopathiae found in pigs. however, this assay has been evaluated with only japanese strains and isolates; therefore, it is unknown whether it could be used in other countries with e. rhusiopathiae strains and isolates of different genetic backgrounds. in our study, the pcr assay was evaluated using chinese e. rhusiopathiae vacci ...201627701077
erysipelothrix rhusiopathiae bacteremia: a challenging diagnosis!erysipelothrix rhusiopathiae, a gram-positive bacillus, is reported to cause for cutaneous infections and endocarditis. we report a case of e. rhusiopathiae bacteremia without severe clinical illness. the patient, a 74-year-old man, is suffering from a chronic lymphoid leukemia (llc). following a trauma, the patient developed a bruise on the left inch. because the site of shock seemed clinically infected, oral amoxicilline-acid clavulanic (aac) treatment was started after withdrawn 1 set of bloo ...201627707676
pathogenic characterization of erysipelothrix rhusiopathiae met-203 type spaa strains from chronic and subacute swine erysipelas in japan.to characterize the erysipelothrix rhusiopathiae met-203 type surface protective antigen (spa) a strains causing swine erysipelas in japan, the nucleotide sequence of the hypervariable region of the spaa gene was determined in 80 e. rhusiopathiae (serotype 1a) isolates collected from pigs with chronic and subacute swine erysipelas in 14 prefectures in 2008-2014. in this study, 14 (17.5%) isolates were met-203 type spaa strains. we confirmed the pathogenicity of a met-203 type spaa strain in spec ...201727773881
evaluation of anti-erysipelothrix rhusiopathiae igg response in bottlenose dolphins tursiops truncatus to a commercial pig vaccine.erysipelothrix rhusiopathiae is the causative agent of erysipeloid in humans and of erysipelas in various animals, including bottlenose dolphins tursiops truncatus, in which an infection has the potential to cause peracute septicemia and death. the purpose of this study was to evaluate the efficacy of using an off-label porcine (er bac plus®, zoetis) e. rhusiopathiae bactrin in a bottlenose dolphin vaccination program by determining the anti-e. rhusiopathiae antibody levels in vaccinated dolphin ...201627786163
bacterial genomics reveal the complex epidemiology of an emerging pathogen in arctic and boreal ungulates.northern ecosystems are currently experiencing unprecedented ecological change, largely driven by a rapidly changing climate. pathogen range expansion, and emergence and altered patterns of infectious disease, are increasingly reported in wildlife at high latitudes. understanding the causes and consequences of shifting pathogen diversity and host-pathogen interactions in these ecosystems is important for wildlife conservation, and for indigenous populations that depend on wildlife. among the key ...201627872617
a dangerous hobby? erysipelothrix rhusiopathiae bacteremia most probably acquired from freshwater aquarium fish handling.erysipelothrix rhusiopathiae is a facultative anaerobic gram-positive rod that occurs widely in nature and is best known in veterinary medicine for causing swine erysipelas. in humans, infections are rare and mainly considered as occupationally acquired zoonosis. a case of e. rhusiopathiae bacteremia most likely associated with home freshwater aquarium handling is reported. the route of transmission was probably a cut with the dorsal fin of a dead pet fish. a short review of clinical presentatio ...201627873166
erysipelothrix rhusiopathiae suppurative arthritis in a 12-year-old boy after an unusual fresh water exposure.we report the case of a 12-year-old boy who experienced recurrent suppurative arthritis of the distal interphalangeal joint after near simultaneous exposure to a frog, a dog bite and lake water. anaerobic cultures of synovial fluid obtained during operative debridement grew small round gray colonies ultimately identified as erysipelothrix rhusiopathiae by routine laboratory tests and mass spectrometry.201727977547
infectious tenosynovitis with bloodstream infection caused by erysipelothrix rhusiopathiae, a case report on an occupational pathogen.erysipelothrix rhusiopathiae is an established animal pathogen, which may cause infections in humans. it is a gram-positive rod and found in the tonsils or the digestive tracts of animals. the bacterium is occupationally related, as usually only people with frequent animal contacts are infected. we report a case of a patient who was admitted with an infectious tenosynovitis with bloodstream infection due to e. rhusiopathiae, and to our knowledge, this is the first report of a tenosynovitis with ...201728056818
development of a duplex pcr for rapid detection and differentiation of erysipelothrix rhusiopathiae vaccine strains and wild type strains.the differentiation of vaccine strains from wild type strains is important for disease control. a duplex pcr for rapid detection and differentiation of erysipelothrix rhusiopathiae vaccine strains and wild type strains was developed based on the dna polymerase iv gene. this duplex pcr was sensitive and specific. the detection results were coincident with that of a single nucleotide polymorphisms based pcr but the detection process was more rapid. in conclusion, this duplex pcr was a useful tool ...201728110776
bacterial microbiota in harbor seals (phoca vitulina) from the north sea of schleswig-holstein, germany, around the time of morbillivirus and influenza epidemics.we present microbiologic findings in harbor seal (phoca; phoca vitulina ) carcasses collected from the north sea of schleswig-holstein, germany, 1996-2014, and interpret results in relation to potential variations caused by phocine distemper virus and influenza a virus mass mortalities. we conducted microbiologic investigations on 2,124 tissue samples from lung, liver, kidney, spleen, intestine, and mesenteric lymph nodes from 549 dead harbor seals of the german north sea. a large variety of bac ...201728139956
erysipelothrix rhusiopathiae recruit host plasminogen via the major protective antigen spaa. 201728201685
erysipelothrix rhusiopathiae bloodstream infection - a 22-year experience at mayo clinic, minnesota.erysipelothrix rhusiopathiae is a facultatively anaerobic gram-positive bacillus found mostly in swine, fish and sheep. e. rhusiopathiae classically causes cutaneous eruptions in butchers, fish handlers and veterinarians. based solely on case reports, 90% of e. rhusiopathiae bloodstream infections (bsi) have been associated with infective endocarditis (ie). to assess the true frequency of ie in e. rhusiopathiae bsi as well as other clinical associations, we performed a retrospective cohort analy ...201728206705
single nucleotide polymorphism genotyping of erysipelothrix rhusiopathiae isolates from pigs affected with chronic erysipelas in japan.over the past decades, erysipelothrix rhusiopathiae strains displaying similar phenotypic and genetic profiles of the attenuated, acriflavine-resistant e. rhusiopathiae koganei 65-0.15 strain (serovar 1a) have been frequently isolated from pigs affected with chronic erysipelas in japan. in this study, using the conventional pcr assay that was designed to detect strain-specific single nucleotide polymorphism (snp) sites found in the genome of the vaccine strain, we analyzed e. rhusiopathiae isola ...201728250289
itraq-based quantitative proteomic analysis reveals potential virulence factors of erysipelothrix rhusiopathiae.erysipelothrix rhusiopathiae is a ubiquitous pathogen that has caused considerable economic losses to pig farmers. however, the mechanisms of e. rhusiopathiae pathogenesis remain unclear. to identify new virulence-associated factors, the differentially abundant cell wall-associated proteins (cwps) between high- and low-virulence strains were investigated through isobaric tags for relative and absolute quantitation (itraq) combined with liquid chromatography-quadrupole mass spectrometry (lc-ms/ms ...201728284755
genome-wide single nucleotide polymorphism analysis identified clonal lineages of erysipelothrix rhusiopathiae responsible for the recent increased incidence of acute swine erysipelas in japan.erysipelothrix rhusiopathiae causes swine erysipelas, an important infectious disease in the swine industry. in japan, the incidence of acute swine erysipelas due to serovar 1a has recently increased markedly. to study the genetic relatedness of the strains from the recent cases, we analyzed 34 e. rhusiopathiae serovar 1a swine isolates collected between 1990 and 2011 and further investigated the possible association of the live koganei 65-0.15 vaccine strain (serovar 1a) with the increase in ca ...201728314730
glyceraldehyde-3-phosphate dehydrogenase acts as an adhesin in erysipelothrix rhusiopathiae adhesion to porcine endothelial cells and as a receptor in recruitment of host fibronectin and plasminogen.erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and human erysipeloid. previous studies suggested glyceraldehyde 3-phosphate dehydrogenase (gapdh) plays a role in the pathogenesis of e. rhusiopathiae infection. we studied e. rhusiopathiae gapdh interactions with pig vascular endothelial cells, fibronectin, and plasminogen. recombinant gapdh (rgapdh) was successfully obtained, and it was shown that it plays a role in e. rhusiopathiae adhesion to pig vascular endothelial c ...201728327178
a novel octavalent combined erysipelas, parvo and leptospira vaccine provides (cross) protection against infection following challenge of pigs with 9 different leptospira interrogans serovars.in the present study the efficacy and cross protection of a novel ready-to-use combination vaccine, porcilis® ery + parvo + lepto, against swine leptospirosis, was investigated. the octavalent vaccine contains inactivated antigens of erysipelothrix rhusiopathiae, parvovirus and 6 leptospira interrogans sensu lato strains of serogroups canicola, icterohaemorrhagiae, grippotyphosa, australis (bratislava), pomona and tarassovi. in this study ninety pigs were vaccinated twice with porcilis® ery + pa ...201528405422
multiplex pcr assay for the simultaneous detection and differentiation of clonal lineages of erysipelothrix rhusiopathiae serovar 1a strains currently circulating in japan.the species erysipelothrixrhusiopathiae displays genetic heterogeneity; however, e. rhusiopathiae serovar 1a strains currently circulating in japan exhibit remarkably low levels of genetic diversity and group into clonal sublineages of lineage ivb (ivb-1 and ivb-2). in the present study, based on whole genome sequencing data, we designed primers for a multiplex pcr assay to simultaneously detect and differentiate the sublineages of e. rhusiopathiae strains. among the one hundred and twenty-seven ...201728637945
serotypes and spa types of erysipelothrix rhusiopathiae isolates from british pigs (1987 to 2015).erysipelothrix spp. cause a range of clinical signs in pigs and at least 28 different erysipelothrix spp. serotypes have been identified. in this study, 128 isolates of erysipelothrix spp. from pigs in great britain from 1987 to 2015 were characterised by serotyping and multiplex real time pcr assays targeting the surface protective antigen (spa) and the main genotypes (erysipelothrix rhusiopathiae, erysipelothrix tonsillarum and erysipelothrix spp. strain 2). all 128 british isolates were chara ...201728720292
isolation and detection of erysipelothrix rhusiopathiae and its distribution in humans and animals by phenotypical and molecular methods in ahvaz-iran in 2015.erysipelothrix rhusiopathiae (e. rhusiopathiae) is generally transmitted into the gastrointestinal tract of animals by the intake of contaminated food or water and causes great economic loss in agriculture worldwide. some of the erysipelothrix spp. are the causative agents of erysipeloid, which is an occupational infection in humans. the aim of the present study was to isolate e. rhusiopathiae from animals as well as the hands of the butchers working in ahvaz, iran, and to determine their suscep ...201728761204
antimicrobial resistance in bacterial poultry pathogens: a review.antimicrobial resistance (amr) is a global health threat, and antimicrobial usage and amr in animal production is one of its contributing sources. poultry is one of the most widespread types of meat consumed worldwide. poultry flocks are often raised under intensive conditions using large amounts of antimicrobials to prevent and to treat disease, as well as for growth promotion. antimicrobial resistant poultry pathogens may result in treatment failure, leading to economic losses, but also be a s ...201728848739
microbiological and molecular identification of bacterial species isolated from nasal and oropharyngeal mucosa of fuel workers in riyadh, saudi arabia.this study aimed to determine the bacterial species colonizing the nasal and oropharyngeal mucosa of fuel workers in central riyadh, saudi arabia on a microbiological and molecular level. throat and nasal swab samples were obtained from 29 fuel station attendants in the period of time extending from march to may 2014 in riyadh, saudi arabia. microbiological identification techniques were utilized to identify the bacterial species isolated. antibiotic sensitivity was assessed for each of the bact ...201728855823
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