Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| divergent signaling pathways regulate il-12 production induced by different species of lactobacilli in human dendritic cells. | recent studies have indicated that different strains of lactobacilli differ in their ability to regulate il-12 production by dendritic cells (dcs), as some strains are stronger inducer of il-12 while other are not and can even inhibit il-12 production stimulated by il-12-inducer lactobacilli. in this report we demonstrate that lactobacillus reuteri 5289, as previously described for other strains of l. reuteri, can inhibit dc production of il-12 induced by lactobacilllus acidophilus ncfm. remarka ... | 2015 | 25977118 |
| lactobacillus reuteri dsm 17938 shortens acute infectious diarrhea in a pediatric outpatient setting. | two randomized controlled clinical trials have shown that lactobacillus (l) reuteri dsm 17938 reduces the duration of diarrhea in children hospitalized due to acute infectious diarrhea. this was the first trial evaluating the efficacy of l. reuteri dsm 17938 in outpatient children with acute infectious diarrhea. | 2015 | 25986615 |
| hypolipidemic effects and safety of lactobacillus reuteri 263 in a hamster model of hyperlipidemia. | we aimed to verify the beneficial effects of probiotic strain lactobacillus reuteri 263 (lr263) on hypolipidemic action in hamsters with hyperlipidemia induced by a 0.2% cholesterol and 10% lard diet (i.e., high-cholesterol diet (hcd)). male golden syrian hamsters were randomly divided into two groups: normal (n = 8), standard diet (control), and experimental (n = 32), a hcd. after a two-week induction followed by a six-week supplementation with lr263, the 32 hyperlipidemic hamsters were divided ... | 2015 | 25988768 |
| biochemical analysis of respiratory metabolism in the heterofermentative lactobacillus spicheri and lactobacillus reuteri. | this study evaluated the aerobic and respiratory metabolism in lactobacillus reuteri and lactobacillus spicheri, two heterofermentative species used in sourdough fermentation. | 2015 | 25996113 |
| in vitro modulation of probiotic bacteria on the biofilm of candida glabrata. | a conspicuous new concept of pathogens living as the microbial societies in the human host rather than free planktonic cells has raised considerable concerns among scientists and clinicians. fungal biofilms are communities of cells that possess distinct characteristic such as increased resistance to the immune defence and antimycotic agents in comparison to their planktonic cells counterpart. therefore, inhibition of the biofilm may represent a new paradigm for antifungal development. in this st ... | 2015 | 26028405 |
| impact of a yogurt matrix and cell microencapsulation on the survival of lactobacillus reuteri in three in vitro gastric digestion procedures. | the goal of this study was to assess the interaction between microencapsulation and a yogurt food matrix on the survival of lactobacillus reuteri ncimb 30242 in four different in vitro systems that simulate a gastric environment. the four systems were: united states pharmacopeia (usp) solutions, a static two-step (sts) procedure which included simulated food ingredients, a constantly dynamic digestion procedure (ividis), as well a multi-step dynamic digestion scheme (s'ividis). the ph profiles o ... | 2015 | 26036146 |
| commentary: probiotic and technological properties of lactobacillus spp. strains from the human stomach in the search for potential candidates against gastric microbial dysbiosis. | 2015 | 26042097 | |
| characterization of the 4,6-α-glucanotransferase gtfb enzyme of lactobacillus reuteri 121 isolated from inclusion bodies. | the gtfb enzyme of the probiotic bacterium lactobacillus reuteri 121 is a 4,6-α-glucanotransferase of glycoside hydrolase family 70 (gh70; http://www.cazy.org ). contrary to the glucansucrases in gh70, gtfb is unable to use sucrose as substrate, but instead converts malto-oligosaccharides and starch into isomalto-/malto- polymers that may find application as prebiotics and dietary fibers. the gtfb enzyme expresses well in escherichia coli bl21 star (de3), but mostly accumulates in inclusion bodi ... | 2015 | 26050651 |
| lactobacillus reuteri dsm 17938 as a probiotic for preterm neonates: a strain-specific systematic review. | prevention of necrotizing enterocolitis (nec) while optimizing enteral nutrition (en) is a priority in preterm neonates. lactobacillus reuteri dsm 17938 (l reuteri) is known to improve gut motility. previous systematic reviews have not adequately assessed the effects of l reuteri in improving feed tolerance in preterm neonates. | 2016 | 26059900 |
| feed fermentation with reuteran- and levan-producing lactobacillus reuteri reduces colonization of weanling pigs by enterotoxigenic escherichia coli. | this study determined the effect of feed fermentation with lactobacillus reuteri on growth performance and the abundance of enterotoxigenic escherichia coli (etec) in weanling piglets. l. reuteri strains produce reuteran or levan, exopolysaccharides that inhibit etec adhesion to the mucosa, and feed fermentation was conducted under conditions supporting exopolysaccharide formation and under conditions not supporting exopolysaccharide formation. diets were chosen to assess the impact of organic a ... | 2015 | 26070673 |
| intake of lactobacillus reuteri improves incretin and insulin secretion in glucose-tolerant humans: a proof of concept. | ingestion of probiotics can modify gut microbiota and alter insulin resistance and diabetes development in rodents. we hypothesized that daily intake of lactobacillus reuteri increases insulin sensitivity by changing cytokine release and insulin secretion via modulation of the release of glucagon-like peptides (glp)-1 and -2. | 2015 | 26084343 |
| the trpv1 channel in rodents is a major target for antinociceptive effect of the probiotic lactobacillus reuteri dsm 17938. | certain probiotic bacteria have been shown to reduce distension-dependent gut pain, but the mechanisms involved remain obscure. live luminal lactobacillus reuteri (dsm 17938) and its conditioned medium dose dependently reduced jejunal spinal nerve firing evoked by distension or capsaicin, and 80% of this response was blocked by a specific trpv1 channel antagonist or in trpv1 knockout mice. the specificity of dsm action on trpv1 was further confirmed by its inhibition of capsaicin-induced intrace ... | 2015 | 26084409 |
| efficient mannitol production by wild-type lactobacillus reuteri crl 1101 is attained at constant ph using a simplified culture medium. | mannitol is a natural polyol with multiple industrial applications. in this work, mannitol production by lactobacillus reuteri crl 1101 was studied at free- and controlled-ph (6.0-4.8) fermentations using a simplified culture medium containing yeast and beef extracts and sugarcane molasses. the activity of mannitol 2-dehydrogenase (mdh), the enzyme responsible for mannitol synthesis, was determined. the effect of the initial biomass concentration was further studied. mannitol production (41.5 ± ... | 2015 | 26084891 |
| [efficacy of racecadotril vs. smectite, probiotics or zinc as an integral part of treatment of acute diarrhea in children under five years: a meta-analysis of multiple treatments]. | despite major advances in treatment, acute diarrhea continues to be a public health problem in children under five years. there is no systematic approach to treatment and most evidence is assembled comparing active treatment vs. placebo. | 2015 | 26089269 |
| modulation of the intestinal microbiota and morphology of tilapia, oreochromis niloticus, following the application of a multi-species probiotic. | the intestinal microbiota and morphology of tilapia (oreochromis niloticus) were investigated after the application of a multi-species probiotic containing lactobacillus reuteri, bacillus subtilis, enterococcus faecium and pediococcus acidilactici (aquastar(®) growout). tilapia (55.03 ± 0.44 g) were fed either a control diet or a probiotic diet (control diet supplemented with aquastar(®) growout at 5 g kg(-1)). after four and eight weeks, culture-dependent analysis showed higher levels of lactic ... | 2015 | 26115752 |
| use of anaerobic green fluorescent protein versus green fluorescent protein as reporter in lactic acid bacteria. | lactic acid bacteria (lab) are commonly used in the production of fermented and probiotic foods. development of molecular tools to discriminate the strains of interest from the endogenous microbiota in complex environments like food or gut is of high interest. green fluorescent protein (gfp)-like chromophores strictly requires molecular oxygen for maturation of fluorescence, which restrict the study of microorganisms in low-oxygen environments. in this work, we have developed a noninvasive cyan- ... | 2015 | 26129953 |
| loss of bone and wnt10b expression in male type 1 diabetic mice is blocked by the probiotic lactobacillus reuteri. | type 1 diabetes (t1d)-induced osteoporosis is characterized by a predominant suppression of osteoblast number and activity, as well as increased bone marrow adiposity but no change in osteoclast activity. the fundamental mechanisms and alternative anabolic treatments (with few side effects) for t1d bone loss remain undetermined. recent studies by our laboratory and others indicate that probiotics can benefit bone health. here, we demonstrate that lactobacillus reuteri, a probiotic with anti-infl ... | 2015 | 26135835 |
| immunobiotic lactobacillus administered post-exposure averts the lethal sequelae of respiratory virus infection. | we reported previously that priming of the respiratory tract with immunobiotic lactobacillus prior to virus challenge protects mice against subsequent lethal infection with pneumonia virus of mice (pvm). we present here the results of gene microarray which document differential expression of proinflammatory mediators in response to pvm infection alone and those suppressed in response to lactobacillus plantarum. we also demonstrate for the first time that intranasal inoculation with live or heat- ... | 2015 | 26145728 |
| probiotic lactobacillus rhamnosus gg therapy and microbiological programming in infantile colic: a randomized, controlled trial. | probiotic lactobacillus reuteri and reduced allergen load may lessen the daily crying of colic infants, but the role of lactobacillus rhamnosus gg (lgg) has remained obscure. | 2015 | 26151493 |
| early colonization of lactobacillus reuteri after exposure to probiotics. | the aim of the present in vivo animal study is to investigate the ability of l. reuteri to colonize the oral flora during infancy. | 2015 | 26161603 |
| effect of probiotic bacteria on oral candida in frail elderly. | the aim of this study was to investigate the effect of a daily intake of probiotic lactobacilli on the prevalence and counts of oral candida in frail elderly patients living in nursing homes. the study had a double-blind randomized placebo-controlled design with 2 parallel arms. the study group consisted of 215 older adults (range, 60 to 102 y) who were enrolled after informed consent. after baseline examination and randomization, the subjects were given 1 lozenge containing 2 strains of the pro ... | 2015 | 26202995 |
| bio-transformation of glycerol to 3-hydroxypropionic acid using resting cells of lactobacillus reuteri. | lactobacillus reuteri grown in mrs broth containing 20 mm glycerol exhibits 3.7-fold up-regulation of 3-hydroxypropionic acid (3-hp) pathway genes during the stationary phase. concomitantly, the resting cells prepared from stationary phase show enhancement in bio-conversion of glycerol, and the maximum specific productivity (q p) is found to be 0.17 g 3-hp per g cdw per hour. the regulatory elements such as catabolite repression site in the up-stream of 3-hp pathway genes are presumed for the au ... | 2015 | 26204968 |
| supplementary heat-killed lactobacillus reuteri gmnl-263 ameliorates hyperlipidaemic and cardiac apoptosis in high-fat diet-fed hamsters to maintain cardiovascular function. | obesity and hyperlipidaemia increase the risk of cvd. some strains of probiotics have been suggested to have potential applications in cardiovascular health by lowering serum ldl-cholesterol. in this work, high-fat diet-induced hyperlipidaemia in hamsters was treated with different doses (5×108 and 2·5×109 cells/kg per d) of heat-killed lactobacillus reuteri gmnl-263 (lr263) by oral gavage for 8 weeks. the serum lipid profile analysis showed that ldl-cholesterol and plasma malondialdehyde (p-mda ... | 2015 | 26234728 |
| biochemical characterization of the lactobacillus reuteri glycoside hydrolase family 70 gtfb type of 4,6-α-glucanotransferase enzymes that synthesize soluble dietary starch fibers. | 4,6-α-glucanotransferase (4,6-α-gtase) enzymes, such as gtfb and gtfw of lactobacillus reuteri strains, constitute a new reaction specificity in glycoside hydrolase family 70 (gh70) and are novel enzymes that convert starch or starch hydrolysates into isomalto/maltopolysaccharides (immps). these immps still have linear chains with some α1→4 linkages but mostly (relatively long) linear chains with α1→6 linkages and are soluble dietary starch fibers. 4,6-α-gtase enzymes and their products have sig ... | 2015 | 26253678 |
| physicochemical properties of a water soluble extracellular homopolysaccharide from lactobacillus reuteri sk24.003. | a water soluble exopolysaccharide was obtained from lactobacillus reuteri sk24.003 and its rheological and structural properties were investigated. the polysaccharide yield reached around 40.8g/l for 48h of fermentation. the purified exopolysaccharide was an α-d-glucan with the molecular weight of 2.50×10(7)g/mol. thermal analysis showed the exopolysaccharide broke down above 200°c, the weight dramatically lost (around 53.0%) from 230 to 340°c and the melting point was 147.7°c with the enthalpy ... | 2015 | 26256197 |
| mixed culture models for predicting intestinal microbial interactions between escherichia coli and lactobacillus in the presence of probiotic bacillus subtilis. | bacillus has been proposed as a probiotic due to its in vivo effectiveness in the gastrointestinal tract through antimicrobial activities. the present study investigates the effects of lactobacillus alone or in the presence of bacillus subtilis ma139 on the inhibition of pathogenic escherichia coli k88. mixed cultures were used to predict the possible interactions among these bacteria within the intestinal tract of animals. b. subtilis ma139 was first assayed for its inhibition against e. coli k ... | 2015 | 26259891 |
| synthesis of oligo- and polysaccharides by lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose. | gtfa, a glucansucrase enzyme of the probiotic bacterium lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1 → 4) and (1 → 6) linkages from sucrose. with respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. it appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharid ... | 2015 | 26281004 |
| changes in vaginal microbiota following antimicrobial and probiotic therapy. | the composition of the vaginal microbiota is known to be important for health. when infections occur, antimicrobial therapy is often poorly efficacious. | 2015 | 26282697 |
| reutericyclin producing lactobacillus reuteri modulates development of fecal microbiota in weanling pigs. | lactobacillus reuteri is used as probiotic culture in food and feed applications; however, strain specific properties of l. reuteri that mediate probiotic activity remain unknown. this study aimed to determine effects of feed fermentation with exopolysaccharide and reutericyclin producing l. reuteri on the transition of the gut microbiome of piglets after weaning. the reutericyclin and reuteran producing l. reuteri tmw1.656 was compared to the reutericyclin negative and levan producing l. reuter ... | 2015 | 26284047 |
| gastrointestinal localization of metronidazole by a lactobacilli-inspired tetramic acid motif improves treatment outcomes in the hamster model of clostridium difficile infection. | metronidazole, a mainstay treatment for clostridium difficile infection (cdi), is often ineffective for severe cdi. whilst this is thought to arise from suboptimal levels of metronidazole in the colon due to rapid absorption, empirical validation is lacking. in contrast, reutericyclin, an antibacterial tetramic acid from lactobacillus reuteri, concentrates in the gastrointestinal tract. in this study, we modified metronidazole with reutericyclin's tetramic acid motif to obtain non-absorbed compo ... | 2015 | 26286574 |
| encapsulation and subsequent freeze-drying of lactobacillus reuteri crl 1324 for its potential inclusion in vaginal probiotic formulations. | probiotic formulations must include a high number of viable and active microorganisms. in this work, the survival of human vaginal lactobacillus reuteri crl 1324 during encapsulation, lyophilization and storage, and the activity of encapsulated and/or freeze-dried bacterial cells were evaluated. extrusion-ionic gelation technique was applied to encapsulate l. reuteri crl 1324, using xanthan and gellan. encapsulated and free bacterial cells were freeze-dried with or without lactose and skim milk ... | 2015 | 26299342 |
| is there a role for probiotics in helicobacter pylori therapy? | the role of probiotics in helicobacter pylori therapy remains unclear. lactobacilli can be shown to inhibit h pylori in vitro. some strains of lactobacilli may exert specific antimicrobial effects. there is no strong evidence of a benefit on eradication rate when probiotics are added to a regimen. despite promising results obtained using compounds of l reuteri and s boulardii, high-quality trials are needed to define the role of probiotics as adjuvant therapy. variables that remain to be studied ... | 2015 | 26314668 |
| diversity of lactobacillus reuteri strains in converting glycerol into 3-hydroxypropionic acid. | the present study aims at comparing the performances of three lactobacillus reuteri strains (dsm 20016, dsm 17938, and atcc 53608) in producing 3-hydroxypropionic acid (3-hp) from glycerol and at exploring inhibition phenomena during this bioconversion. differences were highlighted between the three strains in terms of 3-hp production yield, kinetics of substrate consumption, and metabolite production. with a maximal productivity in non-optimal conditions (free ph) around 2 g.l(-1).h(-1) of 3-hp ... | 2015 | 26319567 |
| properties of lactobacillus reuteri chitosan-calcium-alginate encapsulation under simulated gastrointestinal conditions. | the protective effects of encapsulation on the survival of lactobacillus reuteri and the retention of the bacterium's probiotic properties under simulated gastrointestinal conditions were investigated. viable counts and the remaining probiotic properties of calcium (ca)-alginate encapsulated (a group), chitosan-ca-alginate encapsulated (ca group), and unencapsulated, free l. reuteri (f group) were determined. encapsulation improved the survival of l. reuteri subjected to simulated gastrointestin ... | 2015 | 26415668 |
| social stress-enhanced severity of citrobacter rodentium-induced colitis is ccl2-dependent and attenuated by probiotic lactobacillus reuteri. | psychological stressors are known to affect colonic diseases but the mechanisms by which this occurs, and whether probiotics can prevent stressor effects, are not understood. because inflammatory monocytes that traffic into the colon can exacerbate colitis, we tested whether ccl2, a chemokine involved in monocyte recruitment, was necessary for stressor-induced exacerbation of infectious colitis. mice were exposed to a social disruption stressor that entails repeated social defeat. during stresso ... | 2016 | 26422754 |
| relationships between the use of embden meyerhof pathway (emp) or phosphoketolase pathway (pkp) and lactate production capabilities of diverse lactobacillus reuteri strains. | the aims of this study is to compare the growth and glucose metabolism of three lactobacillus reuteri strains (i.e. dsm 20016, dsm 17938, and atcc 53608) which are lactic acid bacteria of interest used for diverse applications such as probiotics implying the production of biomass, or for the production of valuable chemicals (3-hydroxypropionaldehyde, 3-hydroxypropionic acid, 1,3-propanediol). however, the physiological diversity inside the species, even for basic metabolisms, like its capacity o ... | 2015 | 26428921 |
| prebiotic effect of diosgenin, an immunoactive steroidal sapogenin of the chinese yam. | this study investigated the effect of diosgenin, a yam-derived phytochemical, on the growth of enteric lactic acid bacteria (lab). the in vivo effect of diosgenin on the density of intestinal flora was examined in a murine model of food allergy. oral administration with diosgenin markedly restored the diminished density of faecal lab associated with allergic reactions. the direct effect of diosgenin and several structure-related steroidal compounds on the growth of faecal anaerobes isolated from ... | 2012 | 26434311 |
| pp-12 efficacy of a standardized extract of matricariae chamomilla l., melissa officinalis l. and tyndallized lactobacillus acidophilus (h122) compared with lactobacillus reuteri (dsm 17938) and with simethicone for the treatment of infantile colic. | infant colic is a prevalent physiological event of healthy children under 3 months of age, which can disrupt the child's home environment. despite its benign natural history, sometimes it requires a therapeutic approach. there is limited evidence supporting the use of complementary and alternative treatments. the aim of this study was to investigate the effectiveness of a mixture of standardized extract of matricariae chamomilla l., melissa officinalis l. and tyndallized lactobacillus acidophilu ... | 2015 | 26439577 |
| infantile colic: recognition and treatment. | infantile colic is a benign process in which an infant has paroxysms of inconsolable crying for more than three hours per day, more than three days per week, for longer than three weeks. it affects approximately 10% to 40% of infants worldwide and peaks at around six weeks of age, with symptoms resolving by three to six months of age. the incidence is equal between sexes, and there is no correlation with type of feeding (breast vs. bottle), gestational age, or socioeconomic status. the cause of ... | 2015 | 26447441 |
| expression and purification of the uropathogenic escherichia coli papg protein and its surface absorption on lactobacillus reuteri: implications for surface display system vaccines. | uropathogenic escherichia coli (upec) is one of the most common bacteria that can cause urinary tract infections (utis). unfortunately, no human vaccine against utis has been developed. therefore, it is necessary to develop an efficient and safe vaccine that is able to induce mucosal and systemic immune responses. the use of lactic acid bacteria as a delivery system is a promising method to induce the immune system. | 2015 | 26487922 |
| characterization of the functional roles of amino acid residues in acceptor-binding subsite +1 in the active site of the glucansucrase gtf180 from lactobacillus reuteri 180. | α-glucans produced by glucansucrase enzymes hold strong potential for industrial applications. the exact determinants of the linkage specificity of glucansucrase enzymes have remained largely unknown, even with the recent elucidation of glucansucrase crystal structures. guided by the crystal structure of glucansucrase gtf180-δn from lactobacillus reuteri 180 in complex with the acceptor substrate maltose, we identified several residues (asp-1028 and asn-1029 from domain a, as well as leu-938, al ... | 2015 | 26507662 |
| the efficacy and safety of the probiotic bacterium lactobacillus reuteri dsm 17938 for infantile colic: a meta-analysis of randomized controlled trials. | to evaluate the efficacy and safety of lactobacillus reuteri dsm 17938 for treating infantile colic. | 2015 | 26509502 |
| heat killed lactobacillus reuteri gmnl-263 reduces fibrosis effects on the liver and heart in high fat diet-hamsters via tgf-β suppression. | obesity is one of the major risk factors for nonalcoholic fatty liver disease (nafld), and nafld is highly associated with an increased risk of cardiovascular disease (cvd). scholars have suggested that certain probiotics may significantly impact cardiovascular health, particularly certain lactobacillus species, such as lactobacillus reuteri gmnl-263 (lr263) probiotics, which have been shown to reduce obesity and arteriosclerosis in vivo. in the present study, we examined the potential of heat-k ... | 2015 | 26516851 |
| characterization of the ecological role of genes mediating acid resistance in lactobacillus reuteri during colonization of the gastrointestinal tract. | rodent-derived strains of lactobacillus reuteri densely colonize the forestomach of mice and possess several genes whose predicted functions constitute adaptations towards an acidic environment. the objective of this study was to systematically determine which genes of l. reuteri 100-23 contribute to tolerance towards host gastric acid secretion. genes predicted to be involved in acid resistance were inactivated, and their contribution to survival under acidic conditions was confirmed in model g ... | 2016 | 26530032 |
| immunomodulation of lactobacillus reuteri crl1324 on group b streptococcus vaginal colonization in a murine experimental model. | maternal group b streptococcus (gbs) colonization is a risk factor for infectious disease in newborns. one promising strategy is the modulation of vaginal defense to increase the host's ability to combat infection. | 2016 | 26547516 |
| potential of lactic acid bacteria at regulating escherichia coli infection and inflammation of bovine endometrium. | about 40% of dairy cattle develop uterine disease during postpartum period, causing infertility. some studies indicate that uterine infection, predominantly by escherichia coli in the first week postpartum, is associated with metritis, an uterus inflammation in which the cow fails to completely clear bacterial contaminants. the aim of this study was to evaluate the potential of four lactic acid bacteria (lab) (lactobacillus rhamnosus, pediococcus acidilactici, lactobacillus reuteri, and lactobac ... | 2016 | 26549120 |
| effectiveness of lactobacillus reuteri dsm 17938 for the prevention of nosocomial diarrhea in children: a randomized, double-blind, placebo-controlled trial. | multiple studies of probiotics used to prevent nosocomial diarrhea have provided conflicting results. the effects likely depend on the probiotic strain and/or dosage. the aim of this study was to assess the effectiveness of lactobacillus reuteri dsm 17938 (l. reuteri; daily dose of 1 × 10 colony forming units) for preventing nosocomial diarrhea in children. | 2016 | 26551445 |
| colic in infants. | colic in infants leads one in six families (17%) with children to consult a health professional. one systematic review of 15 community-based studies found a wide variation in prevalence, which depended on study design and method of recording. | 2015 | 26581647 |
| synbiotic approach restores intestinal homeostasis and prolongs survival in leukaemic mice with cachexia. | cancer cachexia is a multifactorial syndrome that includes muscle wasting and inflammation. as gut microbes influence host immunity and metabolism, we investigated the role of the gut microbiota in the therapeutic management of cancer and associated cachexia. a community-wide analysis of the caecal microbiome in two mouse models of cancer cachexia (acute leukaemia or subcutaneous transplantation of colon cancer cells) identified common microbial signatures, including decreased lactobacillus spp. ... | 2016 | 26613342 |
| high-fat-diet-induced obesity is associated with decreased antiinflammatory lactobacillus reuteri sensitive to oxidative stress in mouse peyer's patches. | diet-induced inflammation in the small intestine may represent an early event that precedes and predisposes to obesity and insulin resistance. this is related to decrease of lactobacilli in peyer's patches (pp) revealed in our previous study. the present study aimed to clarify specific changes of pp lactobacillus on the strain level and related biological activity. | 2016 | 26620713 |
| the pan-genome of lactobacillus reuteri strains originating from the pig gastrointestinal tract. | lactobacillus reuteri is a gut symbiont of a wide variety of vertebrate species that has diversified into distinct phylogenetic clades which are to a large degree host-specific. previous work demonstrated host specificity in mice and begun to determine the mechanisms by which gut colonisation and host restriction is achieved. however, how l. reuteri strains colonise the gastrointestinal (gi) tract of pigs is unknown. | 2015 | 26626322 |
| a randomized double-blind placebo-controlled trial of lactobacillus reuteri for chronic functional abdominal pain in children. | functional abdominal pain (fap) is one of the most common diseases, and large percentages of children suffer from it. | 2015 | 26635937 |
| infant colic-what works: a systematic review of interventions for breast-fed infants. | to determine the strength of evidence for commonly used interventions for colic in breast-fed and mixed-fed infants younger than 6 months. | 2016 | 26655941 |
| comparative genomics lactobacillus reuteri from sourdough reveals adaptation of an intestinal symbiont to food fermentations. | lactobacillus reuteri is a dominant member of intestinal microbiota of vertebrates, and occurs in food fermentations. the stable presence of l. reuteri in sourdough provides the opportunity to study the adaptation of vertebrate symbionts to an extra-intestinal habitat. this study evaluated this adaptation by comparative genomics of 16 strains of l. reuteri. a core genome phylogenetic tree grouped l. reuteri into 5 clusters corresponding to the host-adapted lineages. the topology of a gene conten ... | 2015 | 26658825 |
| histamine h2 receptor-mediated suppression of intestinal inflammation by probiotic lactobacillus reuteri. | probiotics and commensal intestinal microbes suppress mammalian cytokine production and intestinal inflammation in various experimental model systems. limited information exists regarding potential mechanisms of probiotic-mediated immunomodulation in vivo. in this report, we demonstrate that specific probiotic strains of lactobacillus reuteri suppress intestinal inflammation in a trinitrobenzene sulfonic acid (tnbs)-induced mouse colitis model. only strains that possess the hdc gene cluster, inc ... | 2015 | 26670383 |
| lactobacillus and lactobacillus cell-free culture supernatants modulate chicken macrophage activities. | lactobacilli are commensal microbes that reside in the intestines of several species, including chickens. structural constituents of lactobacilli are able to stimulate the host immune system. macrophages are crucial players in both innate and adaptive immune systems. here, we investigated the effects of lactobacillus acidophilus, lactobacillus reuteri, lactobacillus salivarius and their cell-free culture supernatants on the pro-inflammatory gene expression profile, nitric oxide (no) production a ... | 2015 | 26679813 |
| synthesis of new hyperbranched α-glucans from sucrose by lactobacillus reuteri 180 glucansucrase mutants. | α-glucans produced by glucansucrase enzymes of lactic acid bacteria attract strong attention as novel ingredients and functional biopolymers in the food industry. in the present study, α-helix 4 amino acid residues d1085, r1088, and n1089 of glucansucrase gtf180 of lactobacillus reuteri 180 were targeted for mutagenesis both jointly and separately. analysis of the mutational effects on enzyme function revealed that all d1085 and r1088 mutants catalyzed the synthesis of hyperbranched α-glucans wi ... | 2016 | 26688101 |
| bio-based 3-hydroxypropionic- and acrylic acid production from biodiesel glycerol via integrated microbial and chemical catalysis. | 3-hydroxypropionic acid (3hp) and acrylic acid (aa) are industrially important platform- and secondary chemical, respectively. their production from renewable resources by environment-friendly processes is desirable. in the present study, both chemicals were almost quantitatively produced from biodiesel-derived glycerol by an integrated process involving microbial and chemical catalysis. | 2015 | 26690945 |
| characterization of intestinal lactobacillus reuteri strains as potential probiotics. | this study was conducted to evaluate the probiotic properties of lactobacillus reuteri isolated from human infant feces (less than 3 months). out of thirty-two representative l. reuteri strains isolated from the infant human feces, nine isolates (i.e. lr5, lr6, lr9, lr11, lr19, lr20, lr25, lr26 and lr34) showed survival in acid, bile and simulated stomach-duodenum passage conditions, indicating their high tolerance to gastric juice, duodenal juice and bile environments. the nine isolates did not ... | 2012 | 26781736 |
| characterization of a reuterin-producing lactobacillus reuteri bpl-36 strain isolated from human infant fecal sample. | a reuterin (3-hydroxypropinaldehyde, 3-hpa)-producing isolate from a human infant fecal sample was identified as lactobacillus reuteri bpl-36 strain. the organism displayed a broad-spectrum antimicrobial activity. the gene (gdh) encoding a glycerol dehydratase subunit was detected by pcr, thus confirming its reuterin-producing ability. reuterin concentration of 89.63 mm/ml was obtained in the mrs-glycerol medium after 16 h of incubation at 37 °c. the reuterin concentration required to inhibit th ... | 2012 | 26782041 |
| influence of prebiotics on lactobacillus reuteri death kinetics under sub-optimal temperatures and ph. | eaten foodstuffs are usually fortified with prebiotic ingredients, such as inulin and oligofructose (fos). the main goal of this study was to evaluate the combined effects of inulin and fos with either suboptimal ph or storage temperature on the viability of lactobacillus reuteri dsm 20016. data were modeled through weibull equation for the evaluation of the microbiological shelf life and the survival time. prebiotics enhanced the microbiological shelf life and enhanced the survival time of the ... | 2016 | 26804428 |
| a prebiotic matrix for encapsulation of probiotics: physicochemical and microbiological study. | this work aims to develop an encapsulated oral-synbiotic supplement by studying the effect of adding inulin in alginate beads and observing its ability to protect three probiotic strains: pediocucus acidilactici, lactobacillus reuteri and lactobacillus salivarius. beads of different inulin concentrations 0%, 5%, 10%, 15% and 20% (w/v) in 2% (w/v) alginate solution were prepared by the extrusion/ionotropic gelation method. polymer distribution within beads was characterised using confocal laser s ... | 2016 | 26805512 |
| antimicrobial properties of a potential probiotic lactobacillus from thai newborn feces. | probiotics are increasingly used to treat infectious diarrhea and antibiotic-associated diarrhea. many probiotic bacteria are classified in general such as lactobacillus and are able to colonize the gastrointestinal tracts of infants. | 2015 | 26817219 |
| safety and acceptability of lactobacillus reuteri dsm 17938 and bifidobacterium longum subspecies infantis 35624 in bangladeshi infants: a phase i randomized clinical trial. | probiotics have rarely been studied in young healthy infants from low-income countries. this phase i study investigated the safety and acceptability of two probiotics in bangladesh. | 2016 | 26832746 |
| structure modeling and functional analysis of recombinant dextransucrase from weissella confusa cab3 expressed in lactococcus lactis. | the dextransucrase gene from weissella confusa cab3, having an open reading frame of 4.2 kb coding for 1,402 amino acids, was amplified, cloned, and expressed in lactococcus lactis. the recombinant dextransucrase, wccab3-rdsr was expressed as extracellular enzyme in m17 medium with a specific activity of 1.5 u/mg which after purification by peg-400 fractionation gave 6.1 u/mg resulting in 4-fold purification. wccab3-rdsr was expressed as soluble and homogeneous protein of molecular mass, approxi ... | 2016 | 26861959 |
| the gram-negative bacterium azotobacter chroococcum ncimb 8003 employs a new glycoside hydrolase family 70 4,6-α-glucanotransferase enzyme (gtfd) to synthesize a reuteran like polymer from maltodextrins and starch. | originally the glycoside hydrolase (gh) family 70 only comprised glucansucrases of lactic acid bacteria which synthesize α-glucan polymers from sucrose. recently we have identified 2 novel subfamilies of gh70 enzymes represented by the lactobacillus reuteri 121 gtfb and the exiguobacterium sibiricum 255-15 gtfc enzymes. both enzymes catalyze the cleavage of (α1→4) linkages in maltodextrin/starch and the synthesis of consecutive (α1→6) linkages. here we describe a novel gh70 enzyme from the nitro ... | 2016 | 26868718 |
| bacterial succession in the broiler gastrointestinal tract. | a feeding trial was performed with broilers receiving a diet of wheat-based feed (wbf), maize-based feed (mbf), or maize-based concentrates supplemented with 15% or 30% crimped kernel maize silage (ckms-15 or ckms-30, respectively). the aim of the study was to investigate the bacterial community compositions of the crop, gizzard, ileum, and cecum contents in relation to the feeding strategy and age (8, 15, 22, 25, 29, or 36 days). among the four dietary treatments, bacterial diversity was analyz ... | 2016 | 26873323 |
| exopolysaccharides of lactobacillus reuteri: their influence on adherence of e. coli to epithelial cells and inflammatory response. | the aim of the study was to characterize exopolysaccharides (eps) originated from lactobacillus reuteri strain dsm 17938 (eps-dsm17938) and l. reuteri strain l26 biocenol™ (eps-l26) and evaluate their influence on adherence of enterotoxigenic escherichia coli (etec) to ipec-1 cells and proinflammatory gene expression. both eps were d-glucan polysaccharides with higher molecular weight (mw), but differing in spatial conformation and elicited variable cytokine profile. eps-dsm17938, relatively lin ... | 2016 | 26876991 |
| glucosylation of catechol with the gtfa glucansucrase enzyme from lactobacillus reuteri and sucrose as donor substrate. | lactic acid bacteria use glucansucrase enzymes for synthesis of gluco-oligosaccharides and polysaccharides (α-glucans) from sucrose. depending on the glucansucrase enzyme, specific α-glucosidic linkages are introduced. gtfa-δn (n-terminally truncated glucosyltransferase a) is a glucansucrase enzyme of lactobacillus reuteri 121 that synthesizes the reuteran polysaccharide with (α1 → 4) and (α1 → 6) glycosidic linkages. glucansucrases also catalyze glucosylation of various alternative acceptor sub ... | 2016 | 26898769 |
| in vitro evaluation of swine-derived lactobacillus reuteri: probiotic properties and effects on intestinal porcine epithelial cells challenged with enterotoxigenic escherichia coli k88. | probiotics are considered as the best effective alternatives to antibiotics. the aim of this study was to characterize the probiotic potential of lactobacilli for use in swine farming by using in vitro evaluation methods. a total of 106 lactic acid bacterial isolates, originating from porcine feces, were first screened for the capacity to survive stresses considered important for putative probiotic strains. sixteen isolates showed notable acid and bile resistance, antibacterial activity, and adh ... | 2016 | 26907754 |
| immortalized mh-s cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication. | the sv-40-transformed mh-s cell line maintains some, but not all, features of primary alveolar macrophages (ams) from balb/c mice. we show here that mh-s cells produce inflammatory cytokines il-6 and cxcl10 in response to challenge with gram-positive lactobacillus reuteri, and to tlr2 and nod2 ligands pam3csk4 and mdp, respectively. in contrast, although wild-type ams are infected in vivo by pneumonia virus of mice (pvm), no virus replication was detected in mh-s cells. interestingly, the surfac ... | 2016 | 26916143 |
| beneficial bacteria inhibit cachexia. | muscle wasting, known as cachexia, is a debilitating condition associated with chronic inflammation such as during cancer. beneficial microbes have been shown to optimize systemic inflammatory tone during good health; however, interactions between microbes and host immunity in the context of cachexia are incompletely understood. here we use mouse models to test roles for bacteria in muscle wasting syndromes. we find that feeding of a human commensal microbe, lactobacillus reuteri, to mice is suf ... | 2016 | 26933816 |
| probiotics for the treatment of infantile colic: a systematic review. | to evaluate whether clinical data support the safety and efficacy of probiotics for the management of infantile colic. | 2016 | 26940647 |
| lactobacillus reuteri inhibition of enteropathogenic escherichia coli adherence to human intestinal epithelium. | enteropathogenic escherichia coli (epec) is a major cause of diarrheal infant death in developing countries, and probiotic bacteria have been shown to provide health benefits in gastrointestinal infections. in this study, we have investigated the influence of the gut symbiont lactobacillus reuteri on epec adherence to the human intestinal epithelium. different host cell model systems including non-mucus-producing ht-29 and mucus-producing ls174t intestinal epithelial cell lines as well as human ... | 2016 | 26973622 |
| transferase activity of lactobacillal and bifidobacterial β-galactosidases with various sugars as galactosyl acceptors. | the β-galactosidases from lactobacillus reuteri l103 (lreuβgal), lactobacillus delbrueckii subsp. bulgaricus dsm 20081 (lbulβgal), and bifidobacterium breve dsm 20281 (bbreβgal-i and bbreβgal-ii) were investigated in detail with respect to their propensity to transfer galactosyl moieties onto lactose, its hydrolysis products d-glucose and d-galactose, and certain sugar acceptors such as n-acetyl-d-glucosamine (glcnac), n-acetyl-d-galactosamine (galnac), and l-fucose (fuc) under defined, initial ... | 2016 | 26975338 |
| systematic review with meta-analysis: lactobacillus reuteri dsm 17938 for diarrhoeal diseases in children. | not all probiotics are equal. | 2016 | 26991503 |
| lactobacillus reuteri strains convert starch and maltodextrins into homoexopolysaccharides using an extracellular and cell-associated 4,6-α-glucanotransferase. | exopolysaccharides (eps) of lactic acid bacteria (lab) are of interest for food applications. lab are well-known to produce α-glucan from sucrose by extracellular glucansucrases. various lactobacillus reuteri strains also possess 4,6-α-glucanotransferase (4,6-α-gtase) enzymes. purified 4,6-α-gtases (e.g., gtfb) were shown to act on starches (hydrolysates), cleaving α1→4 linkages and synthesizing α1→6 linkages, yielding isomalto-/maltopolysaccharides (immp). here we report that also l. reuteri ce ... | 2016 | 26996545 |
| in vivo and in vitro detection of luminescent and fluorescent lactobacillus reuteri and application of red fluorescent mcherry for assessing plasmid persistence. | lactobacillus reuteri is a symbiont that inhabits the gastrointestinal (gi) tract of mammals, and several strains are used as probiotics. after introduction of probiotic strains in a complex ecosystem like the gi tract, keeping track of them is a challenge. the main objectives of this study were to introduce reporter proteins that would enable in vivo and in vitro detection of l. reuteri and increase knowledge about its interactions with the host. we describe for the first time cloning of codon- ... | 2016 | 27002525 |
| eugenol in combination with lactic acid bacteria attenuates listeria monocytogenes virulence in vitro and in invertebrate model galleria mellonella. | listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. crossing the intestinal barrier is the first critical step for listeria monocytogenes infection. therefore, reducing l. monocytogenes colonization and invasion of intestinal epithelium and production of virulence factors could potentially control listeriosis in humans. this study investigated the efficacy of sub-inhibitory concentration (sic) of the plant-derived antimicrobial eugeno ... | 2016 | 27002648 |
| postbiotic modulation of retinoic acid imprinted mucosal-like dendritic cells by probiotic lactobacillus reuteri 17938 in vitro. | lactobacilli are widely used as probiotics with beneficial effects on infection-associated diarrhea, but also used in clinical trials of e.g., necrotizing enterocolitis and inflammatory bowel diseases. the possibility of using probiotic metabolic products, so-called postbiotics, is desirable as it could prevent possible side effects of live bacteria in individuals with a disturbed gut epithelial barrier. here, we studied how lactobacillus reuteri dsm 17938 cell-free supernatant (l. reuteri-cfs) ... | 2016 | 27014275 |
| isolation of lactobacillus reuteri from peyer's patches and their effects on siga production and gut microbiota diversity. | we previously reported that specific lactobacillus reuteri colonized within mouse peyer's patches (pp) effectively prevented high fat diet induced obesity and low-grade chronic inflammation. we further investigated the role of pp lactobacillus reuteri on siga production in rats in this study. | 2016 | 27030202 |
| harvesting the benefits of biofilms: a novel probiotic delivery system for the prevention of necrotizing enterocolitis. | probiotics reduce the incidence of necrotizing enterocolitis (nec) albeit only when administered at high frequency (at least daily). we have developed a novel probiotic delivery system in which probiotics are grown as a biofilm on microspheres, allowing enhanced efficacy with only a single treatment. | 2016 | 27032609 |
| effect of formula containing lactobacillus reuteri dsm 17938 on fecal microbiota of infants born by cesarean-section. | microbiota modulation by probiotics in infants born by cesarean (c)-section is poorly understood. we aimed at assessing the response of c-section-delivered infant microbiota to a formula containing lactobacillus reuteri deutsche sammlung von mikroorganismen und zellkulturen gmbh (dsm) 17938 and comparing it with that of vaginally delivered infants. | 2016 | 27035371 |
| impact of oral probiotics on neurodevelopmental outcomes in preterm infants. | the aim of the study was to evaluate the neurodevelopment outcomes of very low birth weight (vlbw) preterm infants supplemented with oral probiotics for the prevention of necrotizing enterocolitis (nec). | 2017 | 27045204 |
| intestinal removal of free fatty acids from hosts by lactobacilli for the treatment of obesity. | recent findings on the association of gut microbiota with various diseases, including obesity, prompted us to investigate the possibility of using a certain type of gut bacteria as a safe therapeutic for obesity. lactobacillus mutants with enhanced capacity in absorption of free fatty acids (ffas) were isolated to show reduced absorption of ffas by the administered host, attributing to inhibition of body weight gain and body fat accumulation as well as amelioration of blood profiles. consequentl ... | 2016 | 27047743 |
| glucansucrase gtf180-δn of lactobacillus reuteri 180: enzyme and reaction engineering for improved glycosylation of non-carbohydrate molecules. | glucansucrases have a broad acceptor substrate specificity and receive increased attention as biocatalysts for the glycosylation of small non-carbohydrate molecules using sucrose as donor substrate. however, the main glucansucrase-catalyzed reaction results in synthesis of α-glucan polysaccharides from sucrose, and this strongly impedes the efficient glycosylation of non-carbohydrate molecules and complicates downstream processing of glucosylated products. this paper reports that suppressing α-g ... | 2016 | 27052379 |
| evaluation of growth, metabolism and production of potentially bioactive components during fermentation of barley with lactobacillus reuteri. | eighteen bacterial isolates from millet, buckwheat and rye flour were identified as lactobacillus reuteri. genomic fingerprinting (rep-pcr) revealed that they represented five strains and phylogenetic analyses using multi locus sequence analysis (mlsa) showed that all clustered with strains of rodent origin. two strains (su12-3 and su18-3) from different phylogenetic clades were used in fermentations of six varieties of barley, both untreated and heat-treated (with inactivated indigenous enzymes ... | 2016 | 27052715 |
| normal growth of infants receiving an infant formula containing lactobacillus reuteri, galacto-oligosaccharides, and fructo-oligosaccharide: a randomized controlled trial. | the safety of an infant formula containing a new mixture of the prebiotics galacto-oligosaccharides (gos) and fructo-oligosaccharide (fos) and the probiotic lactobacillus reuteri needs to be evaluated. | 2015 | 27057326 |
| lactobacillus reuteri 6475 increases bone density in intact females only under an inflammatory setting. | we previously demonstrated that short-term oral administration of the probiotic lactobacillus reuteri 6475 enhanced bone density in male but not female mice. we also established that l. reuteri 6475 enhanced bone health and prevented bone loss in estrogen-deficient female mice. in this study, we tested whether a mild inflammatory state and/or a long-term treatment with the probiotic was required to promote a positive bone effect in estrogen-sufficient female mice. | 2016 | 27058036 |
| lactobacilli require physical contact to reduce staphylococcal tsst-1 secretion and vaginal epithelial inflammatory response. | italic! staphylococcus aureusbiofilms can be found on vaginal epithelia, secreting toxins and causing inflammation. the co-vaginal species italic! lactobacilluscan alter staphylococcal-induced epithelial secretion of inflammatory cytokines and quench staphylococcal toxic shock syndrome toxin-1 secretion. it is hypothesized that these effects of lactobacilli require direct physical contact between lactobacilli, staphylococci and the epithelium. indeed, lactobacilli only reduced italic! s. aureus- ... | 2016 | 27060097 |
| probiotics in critically ill children. | gut microflora contribute greatly to immune and nutritive functions and act as a physical barrier against pathogenic organisms across the gut mucosa. critical illness disrupts the balance between host and gut microflora, facilitating colonization, overgrowth, and translocation of pathogens and microbial products across intestinal mucosal barrier and causing systemic inflammatory response syndrome and sepsis. commonly used probiotics, which have been developed from organisms that form gut microbi ... | 2016 | 27081478 |
| inclusion of lactobacillus reuteri in the treatment of helicobacter pylori in sardinian patients: a case report series. | clinical studies have shown that bismuth-containing quadruple therapy given twice a day for 10 to 14 days is effective and safe in the treatment of helicobacter pylori infection in sardinia. however, bismuth is no longer available in italy. to report the effectiveness and tolerability of pantoprazole 20 mg, tetracycline 500 mg, and metronidazole 500 mg given b.i.d. (with the midday and evening meals) for 10 days supplemented with lactobacillus reuteri (dsm 17938) 10(8) cfu/tablet once a day for ... | 2016 | 27082621 |
| probiotics [lgg-bb12 or rc14-gr1] versus placebo as prophylaxis for urinary tract infection in persons with spinal cord injury [prosciuttu]: a study protocol for a randomised controlled trial. | urinary tract infections [utis] are very common in people with spinal cord injury [sci]. utis are increasingly difficult and expensive to treat as the organisms that cause them become more antibiotic resistant. among the sci population, there is a high rate of multi-resistant organism [mro] colonisation. non-antibiotic prevention strategies are needed to prevent uti without increasing resistance. probiotics have been reported to be beneficial in preventing utis in post-menopausal women in severa ... | 2016 | 27084704 |
| letter to the editor on 'enhancing vitamin b12 content in soy-yogurt by lactobacillus reuteri, ijfm. 206:56-59'. | 2016 | 27085811 | |
| non-antibiotic prophylaxis for urinary tract infections. | increasing antimicrobial resistance has stimulated interest in non-antibiotic prophylaxis of recurrent urinary tract infections (utis). well-known steps in the pathogenesis of utis are urogenital colonization and adherence of uropathogens to uroepithelial cell receptors. to prevent colonization in postmenopausal women, vaginal, but not oral, estrogens have been shown to restore the vagina lactobacilli flora, reduce vaginal colonization with enterobacteriaceae, and reduce the number of utis compa ... | 2016 | 27092529 |
| characterization of the anti-inflammatory lactobacillus reuteri bm36301 and its probiotic benefits on aged mice. | the gut microbiota is playing more important roles in host immune regulation than was initially expected. since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest. | 2016 | 27095067 |
| lactobacillus reuteri increases mucus thickness and ameliorates dextran sulphate sodium-induced colitis in mice. | the aim of this study was to investigate whether two lactobacillus reuteri strains (rat-derived r2lc and human-derived atcc pta 4659 (4659)) could protect mice against colitis, as well as delineate the mechanisms behind this protection. | 2016 | 27096537 |
| characterization of biosurfactants produced by lactobacillus spp. and their activity against oral streptococci biofilm. | lactic acid bacteria (lab) can interfere with pathogens through different mechanisms; one is the production of biosurfactants, a group of surface-active molecules, which inhibit the growth of potential pathogens. in the present study, biosurfactants produced by lactobacillus reuteri dsm 17938, lactobacillus acidophilus dds-1, lactobacillus rhamnosus atcc 53103, and lactobacillus paracasei b21060 were dialyzed (1 and 6 kda) and characterized in term of reduction of surface tension and emulsifying ... | 2016 | 27102127 |
| lactobacillus reuteri supplements do not affect salivary iga or cytokine levels in healthy subjects: a randomized, double-blind, placebo-controlled, cross-over trial. | to evaluate the effect of daily ingestion of probiotic lactobacilli on the levels of secretory iga (siga) and selected cytokines in whole saliva of healthy young adults. | 2016 | 27104984 |
| screening of lactobacillus isolates from gastrointestinal tract of guinea fowl for probiotic qualities using in vitro tests to select species-specific probiotic candidates. | a total of 32 lactobacillus isolates, 8 each from the crop (lgfcp1-lgfcp8), proventriculus (lgfp9-lgfp16), ileum (lgfi17-lgfi24) and caeca (lgfcm25-lgfcm32) were isolated from 25 adult guinea fowl (pearl variety), 22-28 weeks of age, and characterised morphologically, physiologically, biochemically and by molecular methods. isolates were screened for their probiotic quality using range of in vitro tests: aggregation test, cell surface hydrophobicity, resistance to bile salts and acidic condition ... | 2016 | 27113552 |