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mode of action of n-tosyl-l-phenylalanylchloromethane on the elongation protein-synthesizing s 3 factor from bacillus stearothermophilus. 19734711185
metabolism of phosphatidylglycerol, phosphatidylethanolamine, and cardiolipin of bacillus stearothermophilus.the total phospholipid content of bacillus stearothermophilus was constant during exponential growth, increased during the transition from the exponential to stationary phase of growth, and then slowly increased during the stationary phase. the first increase was a result of an increase in phosphatidylethanolamine; the second was a result of an increase in cardiolipin. cessation of aeration of an exponentially growing culture or suspension in a nongrowth medium resulted in an immediate reduction ...19734712568
preferred sites of digestion of a ribonuclease from enterobacter sp. in the sequence analysis of bacillus stearothermophilus 5s ribonucleic acid. 19734719125
metabolism of phospholipids in bacillus stearothermophilus. 19734756899
isolation and characterization of a thermophilic glucokinase from bacillus stearothermophilus. 19734763322
6-phosphogluconate dehydrogenase from bacillus stearothermophilus. 19734772689
[determination of reaction kinetic data on the vapor inactivation of bacillus stearothermophilus spores, endotrophically influenced by bivalent cations]. 19734774658
mode of action of the macrolide-type antibiotic, chlorothricin. kinetic study of the inhibition of pyruvate carboxylase from bacillus stearothermophilus. 19734775066
letter: crystallization and preliminary x-ray diffraction studies on tyrosyl-transfer rna synthetase from bacillus stearothermophilus. 19734784896
effect of copper on the growth of bacillus stearothermophilus. 19734801279
metabolic differences in bacillus stearothermophilus grown at 55 degrees c and 37 degrees c. 19744815911
amidation and cross-linking of the enzymatically synthesized peptidoglycan of bacillus stearothermophilus. 19744822500
purification and properties of esterase from bacillus stearothermophilus. 19744831626
tyrosyl transfer ribonucleic acid synthetase from bacillus stearothermophilus. preparation and properties of the crystallizable enzyme. 19744831635
thermoadaptation of enzymes in thermophilic and mesophilic cultures of bacillus stearothermophilus and bacillus caldotenax. 19744847049
reconstitution of 50 s ribosomal subunits from bacillus stearothermophilus. 19744854050
studies on the role of polyamines associated with the ribosomes from bacillus stearothermophilus.1. spermine and spermidine were the main polyamines detectable in bacillus stearothermophilus. 2. when grown at 65 degrees b. stearothermophilus contained lower concentrations of polyamines per mg. of rna than when grown at 45 degrees or at 55 degrees . 3. ribosomes isolated from b. stearothermophilus in 0.01m-tris-hydrochloric acid buffer (ph7.4)-0.01m-magnesium chloride contained sufficient polyamines to neutralize between 4% and 9% of their rna phosphorus. 4. removal of polyamines from the ri ...19684875413
renaturation of heat-inactivated leucyl-trna synthetases from bacillus stearothermophilus and escherichia coli. 19684875729
[the electron microscopic fine structure of the cell wall, cytoplasmic membrane and flagella of bacillus stearothermophilus demonstrated by means of freeze etching]. 19684881530
isolation and amino acid composition of ribosomal proteins from bacillus stearothermophilus.twelve of the proteins from the 30s ribosome of bacillus stearothermophilus were isolated by preparative disc electrophoresis. amino acid analyses of these proteins showed them to be different from each other. the gross amino acid composition of 30s ribosomal protein from b. stearothermophilus and escherichia coli are virtually identical. a number of the proteins of b. stearothermophilus had electrophoretic mobilities similar or identical to 30s ribosomal proteins of e. coli. however, there was ...19694891258
surface structure of bacillus stearothermophilus ribosomes.no significant differences were noted in either the size or in the arrangement of the surface filaments of the ribosomes of bacillus stearothermophilus and escherichia coli.19694891269
physiological role of 70s ribosomes in bacteria.evidence is presented indicating that free 70s ribosomes are real components of bacillus stearothermophilus and escherichia coli in exponential and stationary phases of growth. after pulses of radioactive leucine or uracil were given, protein synthesis was instantaneously stopped by chloramphenicol, or polypeptide growth was allowed to proceed to completion in slowly cooled cells. analysis of label distribution in sucrose gradients showed that 70s monomers, and not subunits, are final products o ...19694894332
purification and properties of dna-dependent rna-polymerase from bacillus stearothermophilus. 19694894537
dissociation of 70s ribosomes: some properties of the dissociating factor from bacillus stearothermophilus and escherichia coli.a protein factor which produces in vitro dissociation of 70s particles into 30s and 50s subunits has been obtained from bacillus stearothermophilus and escherichia coli. the factor could be extracted from ribosomes, polyribosomes, and s100 supernatant. the kinetics and temperature curve of the dissociation process in the b. stearothermophilus system have been studied and compared with the reaction in the e. coli system. no species specificity was observed when hybrid mixtures of ribosomes and di ...19694901704
characterization of the bacillus stearothermophilus phage phimu-4 and its dna. 19704910441
production of bacillus stearothermophilus nca 1503 for glyceraldehyde-3-phosphate dehydrogenase (gpdh). 19714933805
mode of action of the competence-inducing factor of bacillus stearothermophilus.development of competence in the noncompetent mutant of bacillus stearothermophilus 4s requires a soluble competence-inducing factor (cf). this study describes several observations that define the mechanism of action of this factor. (i) cf interacts with cells, protoplasts, or cytoplasmic membranes in a temperature-dependent reaction; incubation of cf with cells alters the sedimentation properties of the cells. (ii) association between cf and the cytoplasmic membrane appears to be the first and ...19714934067
factors affecting transfection in bacillus stearothermophilus.the conditions for the infection of bacillus stearothermophilus 4s with tp-1c phage deoxyribonucleic acid (dna) are described. cells from log-phase cultures are the most susceptible to phage dna infection (transfection). a cellular component (competence factor) which enhances transfection is released into the culture medium during the transition period between the log and stationary phase of growth. transfection is stimulated in the order of decreasing effectiveness, by fe(3+), mn(2+), and mg(2+ ...19714934070
temperature dependence of the aminoacylation of trna by bacillus stearothermophilus aminoacyl-trna synthetases. 19714940767
heat stabilities of ribosomal subunits and reassociated ribosomes from bacillus stearothermophilus.absorbance-temperature profiles reveal that both the 30s and 50s ribosomal subunits from bacillus stearothermophilus are more thermostable than the comparable escherichia coli particles. thermophile ribosomes formed by the reassociation of subunits do not display functional heat stability.19714941572
association factor of ribosomal subunits from bacillus stearothermophilus.the isolation of a new factor, which can cause the in vitro association of 30s and 50s ribosomal subunits at low mg(++) concentration, is described. the association factor is eluted together with the dissociation protein when ribosomes of bacillus stearothermophilus are washed with salt solutions of high concentration. the association activity is heat-stable, whereas dissociation factor is inactivated after 10 min at 80 degrees c. this treatment allows the separation of both factors. several pro ...19714941986
bacillus stearothermophilus (donk), a strongly heat-resistant thermophilic contaminant in prepared dehydrated nutrient agar. 19714942975
malate dehydrogenases. ii. purification and properties of bacillus subtilis, bacillus stearothermophilus, and escherichia coli malate dehydrogenases. 19674960671
milk component(s) inhibitory to bacillus stearothermophilus. 19664961201
comparative thermostability of enzymes from bacillus stearothermophilus and bacillus cereus. 19684970342
synthesis of cell-wall mucopeptide by particulate preparations from bacillus megaterium and bacillus stearothermophilus. 19684971163
purification and comparative properties of a pyrimidine nucleoside phosphorylase from bacillus stearothermophilus. 19694978445
thermostable aldolase from thermus aquaticus.data are presented on the purification and properties of the thermostable fructose-1,6-diphosphate aldolase of thermus aquaticus, a nonsporulating, extreme thermophile. the enzyme shows little activity at temperatures below 60 c and optimal activity at about 95 c. the enzyme was purified 43-fold by diethylaminoethyl cellulose column chromatography and sephadex g-200 gel filtration. the enzyme is activated by high concentrations of nh(4) (+) and low concentrations of fe(2+) and co(2+) and is stro ...19704984076
studies on thermophilic alpha-amylase from bacillus stearothermophilus. ii. thermal stability of thermophilic alpha-amylase. 19704984659
purification and properties of a thermophilic bacteriophage lytic enzyme.a phage lytic enzyme was isolated from lysates of bacillus stearothermophilus (nca 1503-4r). the enzyme was purified 1,998-fold with a 27% recovery of enzyme activity. by use of polyacrylamide gel electrophoresis and sucrose gradient centrifugation the enzyme was judged free from protein contaminants. the lytic enzyme was active over a ph range of 6.0 to 7.0, with a maximum at 6.3, and it was stable between ph 7.0 and 8.0 and at 5.0 and unstable between ph 5.5 and 6.5. the temperature coefficien ...19674990043
relative incorporation of phenylalanine at different temperatures by cell-free systems from bacillus licheniformis and bacillus stearothermophilus. 19714993725
thermophilic aminopeptidases from bacillus stearothermophilus. i. isolation, specificity, and general properties of the thermostable aminopeptidase i. 19695001155
intracellular proteases of bacillus stearothermophilus.cell-free extracts of bacillus stearothermophilus have been shown to exhibit proteolytic activity toward casein as well as specific activity to catalyze the hydrolysis of furylacryloylglycyl-l-leucine amide, furylacryloylglycine, and carbobenzoxyl-glycine-p-nitrophenyl ester, indicating the presence of a neutral proteinase, a carboxypeptidase-like enzyme, and an alkaline proteinase. the neutral proteinase and carboxypeptidase-like activities were separated by gel filtration over bio-gel p-60, an ...19715002894
study of two bacteriophages of bacillus stearothermophilus strain nca1518.studies of bacteriophages gh5 and gh8 of bacillus stearothermophilus strain nca1518 revealed that their properties were sufficiently different from known phages and from each other to indicate that each was a different entity, although no major deviation was demonstrated.19725014935
glucan biosynthesis in bacillus stearothermophilus. i. properties of the polysaccharide. 19725017254
elevated diaminopimelate-sensitive aspartokinase activity during sporulation of bacillus stearothermophilus. 19725021985
[l-leucyl-sarcosyl-l-phenylalanine, synthesis and effect on aminopeptidases i and ii from bacillus stearothermophilus]. 19725023591
the phospholipids of a faculatively thermophilic strain of bacillus stearothermophilus. 19725074272
a unified hypothesis on the causes of the cardinal temperatures of microorganisms; the temperature minimum of bacillus stearothermophilus. 19725074610
the location of spermine in bacterial ribosomes as indicated by 1,5-difluoro-2,4-dinitrobenzene and by ethidium bromide.1. when the binding of ethidium bromide to rrna is measured both in the presence and in the absence of spermine, by spectrophotometric titrations, by gel filtration, or by the changes in fluorescence intensity, spermine competes with ethidium bromide for sites on the rrna; under the conditions used in these experiments ethidium bromide is bound to the double-stranded regions of rrna. 2. when an excess of ethidium bromide is added to ribosomes from bacillus stearothermophilus approx. 80% of the e ...19725084791
purification and characterization of glucose-6-phosphate isomerase from bacillus stearothermophilus. 19715117529
biosynthetic l-threonine deaminase from bacillus stearothermophilus. i. catalytic and regulatory properties. 19715123148
synthesis of pyruvate carboxylase from its apoenzyme and (+)-biotin in bacillus stearothermophilus. purification and properties of the apoenzyme and the holoenzyme synthetase.1. methods are described for the assay and purification of pyruvate apocarboxylase and pyruvate holocarboxylase synthetase from biotin-deficient bacillus stearothermophilus. 2. pyruvate apocarboxylase was obtained 200-fold purified and in a nearly homogeneous state; it closely resembled the holoenzyme of the thermophile in fractionation properties, electrophoretic mobility and molecular weight (estimated to be 350000 by gel filtration). 3. pyruvate holocarboxylase synthetase, purified more than ...19715129257
synthesis of pyruvate carboxylase from its apoenzyme and (+)-biotin in bacillus stearothermophilus. mechanism and control of the reaction.1. acetyl-coa acts as a positive allosteric effector in the formation of active pyruvate carboxylase from its apoenzyme, atp and (+)-biotin which is catalysed by holoenzyme synthetase; this effect is counteracted by l-aspartate. 2. the hill coefficients (apparent n values) were approximately 2 for acetyl-coa and 4 for l-aspartate; the n value for each effector remained constant when the concentration of the other effector was varied. 3. active pyruvate carboxylase was formed also when the apoenz ...19715129260
triose phosphate isomerase from bacillus stearothermophilus. 19715131007
rapid quantitative microbiological assay of antibiotics and chemical preservatives of a nonantibiotic nature.a rapid quantitative assay of 14 antibiotics and 6 chemical preservatives by the cylinder-plate diffusion method is described, in which bacillus stearothermophilus atcc 12980 was used as the test organism. after 4 hr of incubation at 65 c, the zones of inhibition can be read off easily with the naked eye. the 4-hr assay was compared under identical conditions with the conventional 16- to 18-hr agar diffusion assay, i.e., by using the test organisms and media proposed by the code of federal regul ...19715132091
the lipid content of bacillus stearothermophilus at 37 degrees and at 55 degrees. 19715146842
catalytic and regulatory properties of meso-diaminopimelate-sensitive aspartokinase from bacillus stearothermophilus. 19715167534
on three complementary amino acid polymerization factors from bacillus stearothermophilus: separation of a complex containing two of the factors, guanosine-5'-triphosphate and aminoacyl-transfer rna. 19685248824
role of 5s rna in the functions of 50s ribosomal subunits.50s ribosomal subunits from bacillus stearothermophilus can be reconstituted from their dissociated components, namely a 5s rna-free protein fraction, a 5s rna-free 23s ribosomal rna fraction, and purified 5s rna. the biological activity of reconstituted particles in polypeptide synthesis is dependent on the presence of 5s rna. in the absence of 5s rna, particles are produced that have greatly reduced activity in (a) polypeptide synthesis directed by synthetic, as well as natural, messenger rna, ...19715289238
enumeration of bacillus stearothermophilus by use of membrane filter techniques to eliminate inhibitors present in milk. 19655325418
interaction between denatured dna and rna from bacillus stearothermophilus involving only one-half of total dna. 19665330748
spore production by bacillus stearothermophilus. 19665332956
protein synthesis in bacillus stearothermophilus. 19665338493
spore formation by bacillus stearothermophilus. 19695358098
[the effect of abrupt temperature shift on the multiplication of bacillus stearothermophilus]. 19695379820
[fine structure of the mesosomes in bacillus stearothermophilus and bacillus coagulans]. 19695398304
studies on thermophilic alpha-amylase from bacillus stearothermophilus. i. some general and physico-chemical properties of thermophilic alpha-amylase. 19705416895
studies on thermophilic alpha-amylase from bacillus stearothermophilus. 3. effect of temperature on the renaturation of denatured thermophilic alpha-amylase. 19705416896
chemical states of bacterial spores: heat resistance and its kinetics at intermediate water activity.bacterial spore heat resistance at intermediate water activity, like aqueous and strictly dry heat resistance, is a property manipulatable by chemical pretreatments of the dormant mature spore. heat resistances differ widely, and survival is prominently nonlogarithmic for both chemical forms of the spore. log survival varies approximately as the cube of time for the resistant state of bacillus stearothermophilus spores and as the square of time for the sensitive state. a method for measuring hea ...19705418938
concerted feedback inhibition of aspartokinase from bacillus stearothermophilus. i. catalytic and regulatory properties. 19705423383
fatty acids from vegetative cells and spores of bacillus stearothermophilus.in spores of bacillus stearothermophilus produced at 45 and 55 c, branched-chain fatty acids predominated in the former and straight-chain acids in the latter.19705429727
[flagellar apparatus in bacillus stearothermophilus and bacillus coagulans]. 19705469673
enzymological differences of alpha-amylase from bacillus stearothermophilus grown at 37 degrees c and 55 degrees c. 19705477223
studies on the subunit structure of thermostable glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. 19705497141
fracture faces in intact cells and protoplasts of bacillus stearothermophilus. a study by conventional freeze-etching and freeze-etching of corresponding fracture moieties. 19705507821
a digital computer program for the statistical analysis of heat resistance data applied to bacillus stearothermophilus spores. 19705516592
chemical and morphological changes in bacillus stearothermophilus induced by copper. 19705526726
turnover and thermostability of proteins and nucleic acids in bacillus stearothermophilus. 19715556683
competence-inducing factor of bacillus stearothermophilus.an incompetent mutant (4s com(-)) does not release competence-inducing factor (cf) into the culture medium and is not infected with tp-1c phage deoxyribonucleic acid (dna) unless cf is added to the transfection assay. a tp-1c phage-resistant mutant (4s ton-r) releases relatively large amounts of cf into the culture medium but is not infected with tp-1c phage dna, even in the presence of cf. the production of cf by the wild type or ton-r mutant and the ability of the wild type or com(-) mutant to ...19715557600
characteristics of tphi3, a bacteriophage for bacillus stearothermophilus.a bacteriophage (tphi3) which infects the thermophilic bacterium bacillus stearothermophilus atcc 8005 was isolated and characterized. infection of the bacterium by the bacteriophage was carried out at 60 c, the optimal growth temperature of the host. at 60 c, the phage had a latent period of 18 min and a burst size of about 200. the phage was comparatively thermostable in broth. the halflife of tphi3 was 400 min at 60 c, 120 min at 65 c, 40 min at 70 c, and 12 min at 75 c. the activation energy ...19675623976
spoilage bacteria in canned foods. i. flat sour spoilage bacteria in canned asparagus and the thermal death time.bacillus stearothermophilus was found to be a cause of spoilage of asparagus canned in taiwan. the f(250) and z values of the isolates were 14.2 min and 17.8 f (-7.9 c), respectively.19685636471
structure of bacillus stearothermophilus: an electron microscope study. 19685649607
amino acid content of rough and smooth variants of bacillus stearothermophilus spores. 19685664118
effect of temperature on growth characteristics of bacillus stearothermophilus. 19685664135
muramic acid phosphate and the linkage of teichoic acid to peptidoglycan in bacillus stearothermophilus cell walls. 19685672129
relief of casein inhibition of bacillus stearothermophilus by iron, calcium, and magnesium.growth of bacillus stearothermophilus strain nca 1518 smooth in dextrose tryptone agar (dta) was inhibited by sodium caseinate. binding studies indicated that sodium caseinate, when present in dta, had the capacity to effect an iron deficiency which could cause inhibition of growth. additions of essential cations, iron (1 mm), calcium (5 mm), magnesium (10 mm), or hydrogen ion (ph 5.7), relieved inhibition. responses to and interactions among these relief factors were analyzed statistically. equ ...19685694503
magnesium requirement in the transacylation reaction catalysed by the isoleucyl-rna synthetase of bacillus stearothermophilus. 19685698896
protein synthesis and ribosomal distribution at different growth stages in bacillus stearothermophilus. 19685722705
[ca++ in the growth and thermoresistence of bacillus stearothermophilus]. 19685734080
[effect of amino acids on spore germination in bacillus stearothermophilus, strain 214]. 19685735974
[effect of carbohydrates and their derivatives on spore germination in bacillus stearothermophilus strain 214]. 19685735993
[fine structure of the cytoplasmic membrane of bacillus stearothermophilus and bacillus coagulans]. 19685745587
phospholipids from bacillus stearothermophilus.the lipids of bacillus stearothermophilus strain 2184 were extracted with chloroform-methanol and separated into neutral lipid and three phospholipid fractions by chromatography on silicic acid columns. the phospholipids were identified by specific staining reactions on silicic acid-impregnated paper, by chromatography of alkaline and acid hydrolysis products, and by determination of acyl ester:glycerol:nitrogen:phosphorus molar ratios. the total extractable lipid was 8% of the dry weight of who ...19695764328
dipicolinate-induced germination of bacillus stearothermophilus spores. 19695764345
the extracellular alpha-amylase of bacillus stearothermophilus. 19695776281
isolation of spheroplast membranes and stability of spheroplasts of bacillus stearothermophilus.spheroplasts were prepared by lysozyme digestion of the cell wall and ruptured by suspension in 0.15 m nacl, followed by centrifugation at 30,900 x g for 35 min, and by a final suspension in 0.05 m nacl for 12 to 16 hr at 5 c. the membrane ghosts were washed four times in tris(hydroxylmethyl)aminomethane (tris) magnesium buffer and once in distilled water. the intact membranes resembled empty sacs with narrow slits in which the cytoplasm was extruded. a 92% recovery of cell membrane was obtained ...19695777801
characteristics of the deoxyribonucleic acid of t phi 3, a bacteriophage for bacillus stearothermophilus.it is shown that the individual strands of bacteriophage tphi3 dna are intact and that heat-denatured tphi3 dna forms a bimodal distribution in a neutral cscl density gradient.19695786184
influence of oxygen on development of nitrate respiration in bacillus stearothermophilus.a denitrifying mutant of bacillus stearothermophilus nca 2184, strain 2184-d, was used to explore the development of nitrate respiration in relation to oxygen respiration. aerobically grown wild-type cultures could acquire the ability to use nitrate as a result of selection of nitrate-respiring mutants by the presence of nitrate and a reduced oxygen tension. fluctuation analysis has revealed that the frequency of occurrence of the nitrate-respiring mutant is about 7.5 x 10(-8) per bacterium per ...19695788697
cell-free amino acid-incorporating systems from bacillus licheniformis and bacillus stearothermophilus 10.cell-free amino acid-incorporating systems were prepared from log-phase cells of bacillus licheniformis and b. stearothermophilus 10. no differences were observed between the two systems with respect to the types and relative amounts of the ribosomes, the time dependence of incorporation, and the optimal magnesium ion concentration (4 mm). the b. licheniformis system showed greater dependence on added transfer ribonucleic acid (trna) and was less stimulated by polyuridylic acid (poly u) than the ...19695788703
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